LOCUS       DACCWV010000001        97356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCWV010000001 DACCWV010000000
VERSION     DACCWV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.001"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            50..1174
                     /locus_tag="IVW84_000001"
     CDS             50..1174
                     /locus_tag="IVW84_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7916237.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1401..2318
                     /locus_tag="IVW84_000002"
     CDS             1401..2318
                     /locus_tag="IVW84_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916238.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2311..3144
                     /locus_tag="IVW84_000003"
     CDS             2311..3144
                     /locus_tag="IVW84_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7916239.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3227..4345
                     /locus_tag="IVW84_000004"
     CDS             3227..4345
                     /locus_tag="IVW84_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7916240.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4339..6237
                     /locus_tag="IVW84_000005"
     CDS             4339..6237
                     /locus_tag="IVW84_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7916241.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            6231..7454
                     /locus_tag="IVW84_000006"
     CDS             6231..7454
                     /locus_tag="IVW84_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7916242.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7540..9474)
                     /locus_tag="IVW84_000007"
     CDS             complement(7540..9474)
                     /locus_tag="IVW84_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7916243.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9700..10356
                     /locus_tag="IVW84_000008"
     CDS             9700..10356
                     /locus_tag="IVW84_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7916244.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10430..10783)
                     /locus_tag="IVW84_000009"
     CDS             complement(10430..10783)
                     /locus_tag="IVW84_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7916245.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            10975..11904
                     /locus_tag="IVW84_000010"
     CDS             10975..11904
                     /locus_tag="IVW84_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7916246.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11915..12751
                     /locus_tag="IVW84_000011"
     CDS             11915..12751
                     /locus_tag="IVW84_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7916247.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            12999..13775
                     /locus_tag="IVW84_000012"
     CDS             12999..13775
                     /locus_tag="IVW84_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7916248.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            13951..14694
                     /locus_tag="IVW84_000013"
     CDS             13951..14694
                     /locus_tag="IVW84_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916249.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14687..16267
                     /locus_tag="IVW84_000014"
     CDS             14687..16267
                     /locus_tag="IVW84_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7916250.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16518..16997)
                     /locus_tag="IVW84_000015"
     CDS             complement(16518..16997)
                     /locus_tag="IVW84_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7916251.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17013..17765)
                     /locus_tag="IVW84_000016"
     CDS             complement(17013..17765)
                     /locus_tag="IVW84_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7916252.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17882..17952
                     /locus_tag="IVW84_000017"
     tRNA            17882..17952
                     /locus_tag="IVW84_000017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17914..17916,aa:Gly,seq:ccc)
     gene            18313..19038
                     /locus_tag="IVW84_000018"
     CDS             18313..19038
                     /locus_tag="IVW84_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7916253.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19293..19559
                     /locus_tag="IVW84_000019"
     CDS             19293..19559
                     /locus_tag="IVW84_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7916254.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19556..19987
                     /gene="ruvX"
                     /locus_tag="IVW84_000020"
     CDS             19556..19987
                     /gene="ruvX"
                     /locus_tag="IVW84_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7916255.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20012..20317
                     /locus_tag="IVW84_000021"
     CDS             20012..20317
                     /locus_tag="IVW84_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7916256.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20398..21543)
                     /locus_tag="IVW84_000022"
     CDS             complement(20398..21543)
                     /locus_tag="IVW84_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7916257.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21604..22251)
                     /locus_tag="IVW84_000023"
     CDS             complement(21604..22251)
                     /locus_tag="IVW84_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7916258.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22445..22738
                     /locus_tag="IVW84_000024"
     CDS             22445..22738
                     /locus_tag="IVW84_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916259.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22782..23093
                     /locus_tag="IVW84_000025"
     CDS             22782..23093
                     /locus_tag="IVW84_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916260.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23090..23714
                     /locus_tag="IVW84_000026"
                     /pseudo
     CDS             23090..23714
                     /locus_tag="IVW84_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24014..24376
                     /locus_tag="IVW84_000027"
     CDS             24014..24376
                     /locus_tag="IVW84_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916261.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24413..25261
                     /locus_tag="IVW84_000028"
     CDS             24413..25261
                     /locus_tag="IVW84_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP7916262.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25251..26726
                     /locus_tag="IVW84_000029"
     CDS             25251..26726
                     /locus_tag="IVW84_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP7916263.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            26992..27399
                     /locus_tag="IVW84_000030"
     CDS             26992..27399
                     /locus_tag="IVW84_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP7916264.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27402..27617
                     /locus_tag="IVW84_000031"
     CDS             27402..27617
                     /locus_tag="IVW84_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916265.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27621..28001
                     /locus_tag="IVW84_000032"
     CDS             27621..28001
                     /locus_tag="IVW84_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7916266.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28135..28290
                     /locus_tag="IVW84_000033"
     CDS             28135..28290
                     /locus_tag="IVW84_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP7916267.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28359..28832
                     /locus_tag="IVW84_000034"
     CDS             28359..28832
                     /locus_tag="IVW84_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7916268.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28829..30523
                     /locus_tag="IVW84_000035"
     CDS             28829..30523
                     /locus_tag="IVW84_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7916269.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30489..30674
                     /locus_tag="IVW84_000036"
     CDS             30489..30674
                     /locus_tag="IVW84_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916270.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30678..31853
                     /locus_tag="IVW84_000037"
     CDS             30678..31853
                     /locus_tag="IVW84_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7916271.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31846..33369
                     /locus_tag="IVW84_000038"
     CDS             31846..33369
                     /locus_tag="IVW84_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7916272.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33425..33709
                     /locus_tag="IVW84_000039"
     CDS             33425..33709
                     /locus_tag="IVW84_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7916273.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            33696..34031
                     /locus_tag="IVW84_000040"
     CDS             33696..34031
                     /locus_tag="IVW84_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7916274.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34326..34952
                     /locus_tag="IVW84_000041"
     CDS             34326..34952
                     /locus_tag="IVW84_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916275.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35694..36530
                     /locus_tag="IVW84_000042"
     CDS             35694..36530
                     /locus_tag="IVW84_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7916276.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            36594..37025
                     /locus_tag="IVW84_000043"
     CDS             36594..37025
                     /locus_tag="IVW84_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7916277.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(37087..38409)
                     /locus_tag="IVW84_000044"
     CDS             complement(37087..38409)
                     /locus_tag="IVW84_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7916278.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38563..40203)
                     /locus_tag="IVW84_000045"
     CDS             complement(38563..40203)
                     /locus_tag="IVW84_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7916279.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40536..41006
                     /locus_tag="IVW84_000046"
     CDS             40536..41006
                     /locus_tag="IVW84_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7916280.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            41228..42331
                     /locus_tag="IVW84_000047"
     CDS             41228..42331
                     /locus_tag="IVW84_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7916281.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            42615..43295
                     /locus_tag="IVW84_000048"
     CDS             42615..43295
                     /locus_tag="IVW84_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7916282.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43385..44419
                     /locus_tag="IVW84_000049"
     CDS             43385..44419
                     /locus_tag="IVW84_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7916283.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44433..44768
                     /locus_tag="IVW84_000050"
     CDS             44433..44768
                     /locus_tag="IVW84_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916284.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            44752..47232
                     /locus_tag="IVW84_000051"
     CDS             44752..47232
                     /locus_tag="IVW84_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916285.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            47288..48847
                     /locus_tag="IVW84_000052"
     CDS             47288..48847
                     /locus_tag="IVW84_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7916286.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            48844..50208
                     /locus_tag="IVW84_000053"
     CDS             48844..50208
                     /locus_tag="IVW84_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7916287.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50379..51449
                     /locus_tag="IVW84_000054"
     CDS             50379..51449
                     /locus_tag="IVW84_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916288.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51464..52132
                     /locus_tag="IVW84_000055"
     CDS             51464..52132
                     /locus_tag="IVW84_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916289.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52152..52730
                     /locus_tag="IVW84_000056"
     CDS             52152..52730
                     /locus_tag="IVW84_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7916290.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53131..54042
                     /locus_tag="IVW84_000057"
     CDS             53131..54042
                     /locus_tag="IVW84_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7916291.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54130..55047)
                     /locus_tag="IVW84_000058"
     CDS             complement(54130..55047)
                     /locus_tag="IVW84_000058"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7916292.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55249..55686
                     /gene="zapA"
                     /locus_tag="IVW84_000059"
     CDS             55249..55686
                     /gene="zapA"
                     /locus_tag="IVW84_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7916293.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            55745..56293
                     /locus_tag="IVW84_000060"
     CDS             55745..56293
                     /locus_tag="IVW84_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7916294.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            56408..58768
                     /locus_tag="IVW84_000061"
     CDS             56408..58768
                     /locus_tag="IVW84_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7916295.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            58855..59169
                     /gene="trxA"
                     /locus_tag="IVW84_000062"
     CDS             58855..59169
                     /gene="trxA"
                     /locus_tag="IVW84_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7916296.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59351..61129
                     /gene="uvrC"
                     /locus_tag="IVW84_000063"
     CDS             59351..61129
                     /gene="uvrC"
                     /locus_tag="IVW84_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7916297.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61274..62173
                     /locus_tag="IVW84_000064"
     CDS             61274..62173
                     /locus_tag="IVW84_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916298.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62166..63428
                     /locus_tag="IVW84_000065"
     CDS             62166..63428
                     /locus_tag="IVW84_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916299.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            63589..65487
                     /locus_tag="IVW84_000066"
     CDS             63589..65487
                     /locus_tag="IVW84_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7916300.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(65817..66536)
                     /locus_tag="IVW84_000067"
     CDS             complement(65817..66536)
                     /locus_tag="IVW84_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7916301.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            66873..67205
                     /locus_tag="IVW84_000068"
     CDS             66873..67205
                     /locus_tag="IVW84_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916302.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67192..69195
                     /locus_tag="IVW84_000069"
     CDS             67192..69195
                     /locus_tag="IVW84_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7916303.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69214..69684
                     /locus_tag="IVW84_000070"
     CDS             69214..69684
                     /locus_tag="IVW84_000070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7916304.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            69705..70289
                     /locus_tag="IVW84_000071"
     CDS             69705..70289
                     /locus_tag="IVW84_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7916305.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70304..71290
                     /locus_tag="IVW84_000072"
     CDS             70304..71290
                     /locus_tag="IVW84_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7916306.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            71280..71591
                     /locus_tag="IVW84_000073"
     CDS             71280..71591
                     /locus_tag="IVW84_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7916307.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            71613..73394
                     /locus_tag="IVW84_000074"
     CDS             71613..73394
                     /locus_tag="IVW84_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7916308.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            73387..74763
                     /locus_tag="IVW84_000075"
     CDS             73387..74763
                     /locus_tag="IVW84_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7916309.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            74770..75405
                     /locus_tag="IVW84_000076"
     CDS             74770..75405
                     /locus_tag="IVW84_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7916310.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            75435..76790
                     /locus_tag="IVW84_000077"
     CDS             75435..76790
                     /locus_tag="IVW84_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7916311.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(76982..78376)
                     /locus_tag="IVW84_000078"
     CDS             complement(76982..78376)
                     /locus_tag="IVW84_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7916312.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            78468..79439
                     /locus_tag="IVW84_000079"
     CDS             78468..79439
                     /locus_tag="IVW84_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7916313.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            79436..80218
                     /locus_tag="IVW84_000080"
     CDS             79436..80218
                     /locus_tag="IVW84_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7916314.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(80387..80758)
                     /locus_tag="IVW84_000081"
     CDS             complement(80387..80758)
                     /locus_tag="IVW84_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7916315.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            80892..81335
                     /locus_tag="IVW84_000082"
     CDS             80892..81335
                     /locus_tag="IVW84_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7916316.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81378..82187)
                     /gene="yidA"
                     /locus_tag="IVW84_000083"
     CDS             complement(81378..82187)
                     /gene="yidA"
                     /locus_tag="IVW84_000083"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7916317.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82206..83573)
                     /locus_tag="IVW84_000084"
     CDS             complement(82206..83573)
                     /locus_tag="IVW84_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7916318.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVIFDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(83557..84387)
                     /locus_tag="IVW84_000085"
     CDS             complement(83557..84387)
                     /locus_tag="IVW84_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7916319.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            84550..84987
                     /locus_tag="IVW84_000086"
     CDS             84550..84987
                     /locus_tag="IVW84_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7916320.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85063..85683
                     /locus_tag="IVW84_000087"
     CDS             85063..85683
                     /locus_tag="IVW84_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7916321.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            85872..86378
                     /locus_tag="IVW84_000088"
     CDS             85872..86378
                     /locus_tag="IVW84_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7916322.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            86395..87294
                     /locus_tag="IVW84_000089"
     CDS             86395..87294
                     /locus_tag="IVW84_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7916323.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            87381..88265
                     /locus_tag="IVW84_000090"
     CDS             87381..88265
                     /locus_tag="IVW84_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7916324.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(88457..88528)
                     /locus_tag="IVW84_000091"
     tRNA            complement(88457..88528)
                     /locus_tag="IVW84_000091"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88493..88495),aa:Arg,seq:ccg)
     gene            88588..89922
                     /gene="rpoN"
                     /locus_tag="IVW84_000092"
     CDS             88588..89922
                     /gene="rpoN"
                     /locus_tag="IVW84_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7916325.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            90125..91162
                     /locus_tag="IVW84_000093"
     CDS             90125..91162
                     /locus_tag="IVW84_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7916326.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            91203..92204
                     /gene="gap"
                     /locus_tag="IVW84_000094"
     CDS             91203..92204
                     /gene="gap"
                     /locus_tag="IVW84_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7916327.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            92314..93504
                     /locus_tag="IVW84_000095"
     CDS             92314..93504
                     /locus_tag="IVW84_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7916328.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            93608..94363
                     /locus_tag="IVW84_000096"
     CDS             93608..94363
                     /locus_tag="IVW84_000096"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7916329.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            94479..95777
                     /gene="eno"
                     /locus_tag="IVW84_000097"
     CDS             94479..95777
                     /gene="eno"
                     /locus_tag="IVW84_000097"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7916330.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            96176..97315
                     /locus_tag="IVW84_000098"
     CDS             96176..97315
                     /locus_tag="IVW84_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7916331.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        32313 a        16636 c        20203 g        28204 t
ORIGIN      
        1 taagtgaaag cgttttatat aagaacttga aaagaaagaa gggatagaca tgaaaaaaag
       61 atacaaattg gtgatagctt tgctaaacat taccctatta ttcagcttaa gtgcatgtgg
      121 aaaaactaac gaagaagtaa cagcaaacat cgcagatcca atgataaaaa aagccactac
      181 attagaagta tatgtcgcaa acggcaaact gacaaactat gttgaaaatg ctgcaaaaat
      241 ctataaccgc aaaactggag caaatattga cttaaaaatt gtgaatgtag cttcgggaac
      301 agcaacagta caaatgatca ccccaaaatt ggtaacacac gaagatatgc cggatattat
      361 ctccatggca gacacttcag cagcaggtgt gctggaaaaa tttcaagatg ctttttatag
      421 tgctgatgaa tatggatttt atgacaaata tggcgatcag ttttatgatc aaaaattgaa
      481 tattttgcgt caacaaacga gtaatgaaac gattattcct tgggctagtg attttacccc
      541 agcactttgt tattatcaac caaaaaaatt tgctgaaata ggtgtagatt ttgaagaaat
      601 cagttcttgg gaagaatata tccaggtggc aaaagcatta aaagaaaaaa ctggtagttt
      661 tggaattgcc ttgccagaat ctggggacca agaattgttt atcaacatgc tagcccagca
      721 gaatcaatct ttgcttgatg aagaaggaaa tatccgtctg aatactaaag aagcgaaaca
      781 tgctgcaaag ttaatcaaag aaatgatcga ctcagatatt gtacatttct acggtgcaca
      841 agatgctgag aaagcatttc aagaatctgc aatgtttgtt gctggcggct ggtatgctac
      901 gaatatgtct ttgaattttc ctgatgcatc tgacaaatgg cgcatggctc cactgattcc
      961 tttttcaaaa gaaaatccag gaaaaggtgc agtatcagga ggatcaagtt tttatgtccc
     1021 taaagatgca aaaaatccac aagtggcaca acaatttctt acatttatgc tgacagatga
     1081 agaatgctta gccaacgcac tggagctggg cgtggctaca tctaactcat tggcctatca
     1141 aacagaagca gctgagaaaa agtttgacta ctaaggagga caaaaatact atgaactatt
     1201 gaataaaatc aatgaaaata catcaaatac tgttttacca gccacttatt cagatgctac
     1261 gtcttatttg actaccgctg catataacta ttggcaaaca ggtgattttg aaaattcata
     1321 tctaaaagaa gcagaaaatt ttgctcataa gtacggcgta aaattaactg gtgattaaaa
     1381 aatattagga ggacttaacg atgatgcaat cttcgttacc aagcaagaca ataaagaaca
     1441 gcaagaaaaa agcaacagat acgtctcata aaaaaagtgc ttatggattg gtcacacctt
     1501 ttgttatttt ctttattgtg acgattttga ttcccatcat tatctcgttt gcttttagtt
     1561 ttatgaaaat tggctactca tttgaatttg ttggatttga caattatcta gctatcttcc
     1621 gagatccatt gtttattaaa tcttacacga atgttttgat tatcatggca ggatcaattc
     1681 caataacgat gctattagct atttactttg ctgttttatt gaattcagca cagttaaaag
     1741 gaaaaggatt ttttagaact gtctattata ttccaaccat tacatcaacg gtggctgtag
     1801 cttccgtatt catgactttt ttcaatccga ctgggttatt caatggattt ttgaatgcgc
     1861 taggaattaa cggtgtacag tggttaacaa atcctttttg gatacgaata tcaatgatca
     1921 tcacgatgat ttggcttagt gtaggatata acactatttt atttttagct ggtcttcaag
     1981 ggatttctaa agagatatac gaaagtgctg aaatagatgg agcatcgaaa tttcggcaat
     2041 tctttttatt aacgatcccc atgctaaagc caatcatttt aatggccaca gttttagcca
     2101 ctatcaatgg cttgggtaat ttcaatatcc caaatatatt ctttgggact tccaatggac
     2161 cagaaaattc agctttggtc gtaggagtca atttatataa aacaagtttt gaaatggttg
     2221 attttggaaa agcctctgct attgcctgga cgatggtgtt tgtatctgtt atattatcgg
     2281 ctattcaatt taaattcgga ggtgataaaa atgactaaac aaaagataca aagcagaatt
     2341 tttacgtacg tcattattgt gtttggtgcc ttaatatttt tgatgccata tttatatatg
     2401 attgtagctt caacgcaaag taatcaagaa attttaagtg gcggaatcaa tttcaagttt
     2461 ggggatatgc tattaaaaaa ctggcaagcc atcatggcga gatataatta tcccttgatt
     2521 atttggaatt cgattgttat tgctgtaatc agtacagttt tagctacagc cagttctaca
     2581 atggcgggtt atgcattagc aaagtatcgt tttatgggga ataaactgtt gttcaataca
     2641 gttatgctat ctaggatggt gccttttttt accacattga ttcctttatt ttacattatg
     2701 agtcgtttag gattatcgaa tacttatgca ggtgtgatca tcccttcgat tgccagtaca
     2761 acttcagtgt ttatgatgcg acaatatagc agtcaatttc ctacagcact gatggaggct
     2821 gccagaatcg atggtgccaa tgagtgggtg atttttacga aaatcgctgt accgatttta
     2881 tcacctacga ttattactag tggtttactt atctttatgg gaagttggaa tcaatatcta
     2941 tttccattag tcatgctttc agataaagat aaatatacgg ttcccttaat tatcaaaaat
     3001 atgacggttt ctagtacagg tgagccaatt aattatggcg caattatgct aattttagca
     3061 acttcagtga tcccaatggt cttgatttat gtgtgggcgc agtctaaatt taaggagaat
     3121 gacattggag ctgcaaataa gtaaaaaaaa taacgaatgt taaccatcta gttcgttcta
     3181 ggagcaactc cacattcata aaaattaaaa gaatggcggg atatccatga atgaacagac
     3241 gaaggaatgg tcaagaaaaa ttttgaatgt agcttctgaa aagatagatg cggaattaga
     3301 cagaatgggg aataaaattc catatatttc ttacaacgga aaatatacag acgtaataaa
     3361 ggacagagga atcgactggt ggaccaatgg tttttggagt ggtattttat ggcagctctt
     3421 tcacaagaca aaacgagaaa agttcaaaga ggcagcaata caacaagaac aattattaga
     3481 atctgccctg taccattttt cagacgttca tcatgatgta ggttttttat tcttaccatc
     3541 ggctattgca cattatcaac agtctcaaaa caaacatagt cgacaactag ggttacatgc
     3601 agctacggta cttgctggac gctataatat ttcagggaac tatttagtag catggaatga
     3661 ccataaacct ggttggatga ttattgatag tatgatgaat ttaccattac tgtattgggc
     3721 ttcgagggaa actggtgatc cgaggttctc taaaattgct cggcagcatg cagatacaac
     3781 agctaagtat ctagtgagga aagatggatc aaccgggcat atcgcagaat ttgatgcaga
     3841 atcagggaaa tatattcggt tacttggcgg gcaaggatac aatgagaatt cttgttggag
     3901 cagaggaaat gcatgggctt tatatggatt cgcgttaagt tatcgttaca caaaaaatcc
     3961 gtactatcta gctatagcag aaaagatagc agtaaatttt attcagaaaa tcgaaaaaac
     4021 tggttatatt cctctcattg attttgctgt aaaagattcg aatattgata ccagtgctgg
     4081 agtatgcgct gcatgcggtc ttctggaatt agcaggatat gtttcctctg tacgatcaaa
     4141 aatgtatcat gaagctgcta tcaatatact ccaaaagaca gaaaagcact atgcaaattg
     4201 gaatatggat gaagatggga tcattggagg cggagcagaa gcataccata gagaactgac
     4261 ttataatgtg ccactgatct attctgatta tttttttatt gaaggcttac ttcgattaga
     4321 acagtcggaa ttagctattt ggtagtaaaa agaggagaaa atacaatgaa acatccagta
     4381 ccactattaa ggaccagaca ggattacttt gacttattta aaaagataaa tcaaccactc
     4441 cagccgtttt atcgagcaag tggcgcacaa attgattata tacatcaagg ggtaggatat
     4501 ggaaataaaa ttgcaggaat ggaaggattc tctcgggttt tatggggagc ggggcccgca
     4561 attgaccaat tagatgaacg atggttgaaa ttaattctat caggaataaa agctggaaca
     4621 gatccttccc atcccgatta ttggggagaa atacatgaaa gagaccagcg tatggtagaa
     4681 atgcccgcaa tgttattagc gttatatcat catgaacaat tattatggaa aaaattagat
     4741 acaggagaaa aaagccagat tattcattgg ttctcgcaaa tttttgaata tgaatgtgca
     4801 gatggtaatt ggcaattttt taagataatt gtcggaacaa ttatccaaaa atttggttat
     4861 ccagttaaag aatcagcact gaaagaagca tttgataata tagaaaattg ttatcttcaa
     4921 aatggctggt atcgagacag ttcaagaggt cgccaagatt attataatcc ttttgcattt
     4981 cattattatg gtttgattta tagcgtacta gaaccagaaa agcccattag ccagatatat
     5041 aagaaccgag ctattgattt ttctagagac tatattcatt tttttgcgga agatggtgca
     5101 aatgtgcctt ttggtcgaag catgatttat cgttttgcag tttcaagctt ttggtcagct
     5161 atgttgtggg ccgatctttt gccttttaaa ttaggtgaaa tcaaggggtt agtcaatcgg
     5221 aatataagat ggtggatgca aaaagaaata tttgatgaaa aaggtatcct gaatattggt
     5281 tatacctacc ctcaattgac attgactgaa ccctataata gtacgttatc tccattatgg
     5341 ctgaataaga tttttttatt gttggcgcta cctgaagacc atgagtattg gacggctaaa
     5401 gaaatgccta tgcctattcg tgaaggaact aaacttcttt cagaagccaa tttgctcata
     5461 caacatgata atggacatac attttttttg aatgcgggac aattagggcc aaatttccat
     5521 acattgacca atgaaaaata tttaaaattt gcttattcta gtcaatttgg tttttcgata
     5581 ccgcgagcga atcaattgaa agcagaaatg gcaatggatt caatgctagg gattcagcga
     5641 gcggacacat atatcacgac cagttttaag aatgagagca tcaaaaccta tggacagttc
     5701 atcgttcgaa ataatgtacg agacattgta tgtaagaaag aggtacttgc ctctacatgg
     5761 gaacctactt tacaggcaaa aatcagaact tggcttattc cttttgaagg ctggcagatt
     5821 cgcgtacaca aagtagaatt agatacagaa tatgttctat atgagacagg gtttgctatt
     5881 gaatgttctc ctgagaaaga aggaataatc atagaagaag acaaggaaaa ctatagggtg
     5941 agtgaagctg gcttttcagg tattatctgt ttaagtgacg atactattaa acgaaaatcc
     6001 acagctatta tgggtttgcc caatacaaac ttgatgacat gggaaaacac ttttattcct
     6061 ggattagagg gtacatttgg aaaaggaacc cattggctcg gaacaggagt ggttgctcat
     6121 caagatcggg attatagttt agaagtatgg aacaatcgtc ccaaaattga ttttgatggg
     6181 acaaccgggc taactatcca aaataaaaac aataaaaaaa gactaaaatt atgctaaagg
     6241 ctggtgggga aatgcttgat aaagtgatgt ttgaagagat aatacatcca gaaaaatgga
     6301 taaatgaatg ttataaagaa ttaatggaaa aagaatggaa taatgagatc gagcgttgtc
     6361 gcagacacac ggagaggtta atcaaacgta tcagtaacag tcagtatcaa ttcccaagcg
     6421 agtgtcctat aaaaggacgt tatccattaa gagacaatat tggttggaca gaaggatttt
     6481 ggacaggaat taattggttg ttatttgaga agacgggtga tcctttattt caacaaacag
     6541 ctgaaaaagc agatatagat tttatccaca ggattgactg tcaaattatc actaatcatc
     6601 acgatcttgg ttttttgtat tctttatcaa ccgtagcaga ttataaaata acgggaaatc
     6661 gaaaaatgaa ggaagcttca attgatgctg cagaaacatt acttcagcgt ttttgggaga
     6721 aggcaggagt tatacaagct tggggagatc ctagtgatcc ttcacaacaa ggtagaatga
     6781 tcatggattg ttgcatgaat cttccattat tgtattgggc gagtgaagaa actggaaatc
     6841 aaaaatataa aaaagctgct caggctcata ttcaaaatgc agcagattat ttagtaagag
     6901 aagatggttc aacttatcat acattttata tggatgtcaa agatggaaaa ccaagatatg
     6961 gaagtacgca tcaaggttat agtgataatt cctgttgggc tagaggccaa gcatggggga
     7021 tttacggttt tgcattaagt tatcgttatt tgaaagaaca aaaatatttt gatttaagca
     7081 aaatattagc caattatttt atgaatcggt tgccagaaga taccatttgt tattgggatt
     7141 tgatctttga tgattctagc agagaagaaa aagatacatc tagtatggtg atcatggttt
     7201 gtggattatt agaattaata gaatcaggtc tattagacaa aacagaaaag aacttatacc
     7261 agtctgctgt ttggaaaatt atgcgacaat tgaaagaaag atatgctaca acggaagaag
     7321 aagatggctt tttaacacag tctgtttata ataagcccaa aaataatggg gtaaatacac
     7381 cttgtatttg gggtgattac tattatttag aagctttagt gagaatgact agtaattggt
     7441 cttcttattg gtgaaaaagg taaatatgga atttttgttt gataaaataa aaaaagaaaa
     7501 accaaaacag catgaatggt ttctgttttg gttttttcgt taattttcat attcttctaa
     7561 ttccaagctt agttcttccc attccgtcaa ttttgtttct tgatcttgtc tagcagcttc
     7621 taaatcttgg ttcagctcat tgagttttac gtgatcttcc aggttttctg gcgcgaccat
     7681 ttcttgttcc aactgatcga tccattcatc gatccgcgcc atttcttctt caacctgagt
     7741 aatttttcgt tgaaggctac ggataagctt ttgctgttct ttgctttggt aataatcaga
     7801 tttgttcgtg ggagcaggag cagattctgg ctcatcttgt gccaacaatt cggcgatttc
     7861 ttcttcttcc tgctttttct ctaaataata atcatagtct cctagataga ctttgctgcc
     7921 attttcagag agttcgacga cttttgtcgc gatccgattg atgaaatatc ggtcatggga
     7981 aacaaaaagc agtgtgccgt cataatcgat caatgcattt tctaatactt ctttgttgtc
     8041 aatatctaaa tggtttgttg gttcatccaa aatcaaaaaa ttttctttgt tcatcgctaa
     8101 cttggctaaa gcaacccgtg ctttttcacc gccacttagc agagggattg gtttttctac
     8161 atcttcgcca gaaaataaga aagcacctaa agcacttcgg atctcttttt ctggagtggt
     8221 aggatgctca tcccacaatt ctgctaggat cgtcttgttt gtatgcagat cagcttgacc
     8281 ttgatcgtaa tagccgatgg tgacatttgt ccctagcgtt tcttttccac ggataaaagg
     8341 gatctggtga atgattgatt tcagcaacgt cgatttcccg ataccattcg gacctactag
     8401 agcaatcgct tcttgacgac ggatatctag attgattggt tcagataagg tgacattatc
     8461 atatccgata gcggcatctt cgatttgcaa gactacattt ccagaaggct tttctatact
     8521 gaatagaaag tttgcttgtt tttcatcccc ttgaggacga tccagccggt ccattttttc
     8581 taatgtcttt ctgcggcttt gcgctcgttt tgtggtagag gcgcgaacaa ggttccgagc
     8641 aacgaagtct tctaatttat ggatttctgt ttgctgcttt tcataagcct tccattcact
     8701 tgctaactgt tctgctttca aatctagata tttcgaatag ttacctttgt aatggcgcat
     8761 ttttcttctg cttaattcat agacttcatt gactacttta tctaaaaaat aacggtcatg
     8821 ggaaacaatc aataatgcac cagtatagct ttgcagataa ttctcaagcc aggaaagtgt
     8881 ctcgatatct aaatggttcg ttggttcatc tagaatcaaa atgtcagggt gttgcaaaag
     8941 catgcgagct aaagctaaac gtgtgcgctg tccgcctgat aaagtatcga ttttctgatc
     9001 ataaaatgaa gaatcgaatt taaacccatg caatacggaa cgaatttcat tttcataacc
     9061 atagccgttt cgatctgaaa aatcgtgttg cagccggtcg tattctttta aataagaact
     9121 ataagcaggt gtgtctggcg taccttcgct aatgaaaatc tctagctcgc gcatccgctc
     9181 ttccatttgt ctaacttgtt caaaagcttt cagcatctcg ttccaaactg tttcattgga
     9241 atcaagtcct gtgtcctgag cgaggtatcc gagagtcgct gttttatttt tagcgatctt
     9301 tccttcatca ggtgcttcaa ttccagcaat gatctttaat aaagtcgatt ttccagcgcc
     9361 atttctgcca actaaagcga cacgagcgcc tgtactgatt tctaaatgta tgttttcaaa
     9421 taagacttct gcaccaaaat ggcgagcaat ctgattggct tgtaataata tcatggaatc
     9481 ctcttttcta ctttgtgaat tctcctcaaa taaaaaacta tttttcaatt agtagtctac
     9541 cataaattcc attatactgc gagatttgca gaaatgataa ataaatgaat aaagaataaa
     9601 atttcacaat atctgagcaa tggaattgat tttttcctat aaatactgct atgatggaga
     9661 aatcaaagat gtaattagac aatacgtgga ggtcatttcg tgaaagatca tattattcca
     9721 aaggcgacag ctaaaagact gcctttgtat tatcgctatt taaggatttt aaataatgct
     9781 ggaaagaaaa aagtgtcttc aactgaatta agtgaagccg ttcaagtaga tagtgcaacg
     9841 atccgtcgag atttttctta ttttggcgaa ttaggaaaac gtgggtatgg ttatgatgta
     9901 gaagatctta tgcacttttt tgcaaagaca ttaaacgacg atcaattgac aaatgttgcg
     9961 ctgatcggtg tagggaactt gggaagtgct ttactaaaat acaaattcca tcaaagtaat
    10021 agcatacgta tcagttgtgc ttttgatgtg aatgaggaaa tcgttgggcg catcgtagac
    10081 ggtatccccg tttatccaat ggaagatatg tcagaacaaa tcaagatcca acaaatcgaa
    10141 gtagcaattt taacgatacc cgctgaaaga gcacaagaag tagtcaatca gttaacggaa
    10201 gcaggaatca aaggaatctt gaactttacc gctgctcgat tgaatgctcc gtcagatgta
    10261 ttgatccaaa atgttgattt gacgaatgaa ttgcaaacct tgatctattt cttgcattct
    10321 gacgcaaatg cgcattccga aaatacagca gaataaaaat aaaaagcgat tgaacaaatg
    10381 aaggcaaaac tttcacttgt tcaatcgctt ttttctgttt ggacaacgat tattttgttc
    10441 ggaagatttc ttcataagaa agggaatcat cttctgctgt cagttcatct agtagataac
    10501 ggattttttt aaagtcgatg aaccgtgctt gccgaaagac ttcttttcct tgatgaaaaa
    10561 tcaggacaac tggcgcagtc agcacttcaa atttactagc tacttctggt gtttcaaatg
    10621 catccaaatg aagtgccgga atatcataat gtgtgagaag ttcctctagt cgtggacgaa
    10681 cagcgtgaca aacactgcaa ttaggcatcg aaagataaat gacggcaacg gatgactgtt
    10741 gcagtatgtc ttgaacttca gtcaagttag tggtttgttt catttttatt ctccctttta
    10801 tgatagaagt gatatgtatg agaccttaat ttaatgggaa taacagaaaa agtcaattga
    10861 agtgctttgg taaaaaataa gagttgtgta aaaatttaca gagcaacttg ttttttttga
    10921 ataaaagcgt atatttgcaa agcgaatgaa ctagaaataa aaaggaggaa actcatgttt
    10981 tcttttattc caaattcggt tttgcagatg agtacgattt tcttatcgat cgttattgaa
    11041 gcattacctt tcgtattatt aggctgtctt atctcaggcg ccttacaagt atttttgacc
    11101 ccagaacgtg tgaagagatg gcttcctgaa aatcgtatct tctctatttt tactggttcg
    11161 atcttaggat ttttctttcc ttcatgtgaa tgcggcatcg taccgatcgt tcatcaattt
    11221 gttaaaaaag gcgtaccagt tcacacagcg tttgctttta tgctgactgc tccgatcatc
    11281 aatccaatcg tgattttttc cacttttatc gcatttggca attcttggca gatggcaagc
    11341 tggcgggtga taggcagctt ttttgttgcc cttgtgattg gtgtctggct tgcctatttt
    11401 caaaaagacg ctgtattgaa agaaaaaatc acgtctaaag tagcagaaaa tcaccatcat
    11461 catgtccatg aagaagaaca aagtgagaag aatgaaccaa aaaatcagct gatccatcaa
    11521 actgggcatg tgttgacaca ttcaatcgac gaattttttg atacaggtcg ttatttgatc
    11581 attggcggat taatcgcagc tgcgatgcag acttatttac caactagaat tatgctaacg
    11641 ctcggctcaa caaagatttt agccattatc atcatgctgt tattggcctt tacgatgtct
    11701 ctttgctcag aagcagatgc gtttatagga tcttctttgt taagtctgtt cggcacagca
    11761 cctgtagctg cctttttggt ctttggccca atggtcgata tcaaaaatct actgatgatg
    11821 aaacgatatt ttacaggacg ctttatttta tcgctgatcg gactgattgc ccttagcgtt
    11881 attggattca cattatttat ttaggaggga aaatatgctg cggtttttga ttttatctgg
    11941 gtatttcgaa ttgatgatgt acttgcaagt ttctggcaaa ctcgatcaat atatcaatgt
    12001 ccattatcgc tacttagcta ttctttcaat gattttatct gctcttttgg ctttagttca
    12061 gctgtatttg tgggtcaaaa acggaaaagc agaagaaaag gcacacgagc acgatcatga
    12121 ccatgggttg tcaaaacctt atcaacgtgg aatcgcttat gtactgctag ctttgccagt
    12181 aatcgtgggg acgttgtttc caacagttag tctcgacact acgatcgtag aagccaaagg
    12241 atttaatttc ccagtaagta aagaatcagt aggggatccg gagatgcaga cacaatactt
    12301 aaagccggat accagtattt atttcaacaa atcagactac ttagaccaaa tgaatgagtt
    12361 gaaagaaaag tatggggata agcaaactat caagatcaca gatgaaaact atttagaagt
    12421 tatggagcta atttataatt accctagcga attcattggc aaaaaaatca gctatgaagg
    12481 atttgtttac caaacaccga acgatagcaa tacagatatc ttcttgttcc gattcggcat
    12541 tatccattgt gtggcagatt caggagtctt tggtttgttg acgcatatgc cggaaggagt
    12601 aacggttaaa aatgatgaat ggtataaaat agaaggaacc atccaatcag actattatca
    12661 accattcaag cgtgaaatcc catctgtagt tgttacaaac gctgagaaag tctctgctcc
    12721 gaaaaatcag tatgtgtatc gttcttttta aaagaacaaa ataaataaac atagctcaga
    12781 cagctgacac gcaaggacgt cggttgtctg gctttttttg catgatgtat tcatttgttt
    12841 gatatttatt ttagaagcag catcctgaaa gtatttgata tcggtttcaa aggtgttgga
    12901 aaagattgtg aagagaatta caatttattc ctaacatgta tgttgaaaag ttgtatgaaa
    12961 tgattttgat agtaaaatat cgagtggagg tactttttat ggtaacgcgt atatttattt
    13021 tggatgatag tttggttttt gaaaaaatgc tagtagaaat tttggaagaa tccagaattt
    13081 tattaagtcc ttggaaattt gagatcgttc agggattgaa cgttatacaa tttgttgaat
    13141 tcattaaaaa taatgaaatc tattcctcag atattttttt cctagacatc gatttgaacg
    13201 cctattatag cggtatcgaa ctaggcgaaa tgcttagaga aaagaatgaa aagtgtaaaa
    13261 ttgtttatct aacgtctcat ccgaataaag caataactgt tattaatcga aagattacgc
    13321 ctagtggtta tttgataaaa gaattcactt atgaaaaaaa taaaaaaagc atccaaagtt
    13381 acttattgaa agaaaaagaa aactatcctg ttcaacatgc tacgaaaaaa aggtggacaa
    13441 ctgtccgtta tggtaatagc gatcactata tccattacga tgaaatcctt tatctaatga
    13501 gcatgccagg ttataaaaat aaaataggcc tagtattgaa agatgaagag attctcgtaa
    13561 acggcacaat taaaaaatat aaaaaatcac tcgaatatga gtatttatgt aaagatttaa
    13621 aagcttatat cattaatact gaaaacatta catctattag cagagtaaac gaaatagtaa
    13681 catttgcaaa tggagaacaa ctagagatgg gaaagagaag tattgataaa ctaaagaatt
    13741 ttatgcaaga aaagatagaa aatgaatcat tttaaagaaa acaaagactc atatcttacc
    13801 tgttagaaga gtgttttcct gtagaagaga acaacccaag tagcagccta gtcgtttcta
    13861 tccttttttg ttatgcactc tttttgacat ttactccacc aataacgaag ggtacaataa
    13921 aaagtaataa caaacaaaag gagtacagaa atgattgaat tcatcaatgt aagtaaaagt
    13981 tacgggaaaa aacaagcatt aaaagacttg tctctctcga taagacaagg ggaaattttt
    14041 ggatttttag gacataatgg tgccggaaaa tcaactacga ttaaaagttt agttagtatt
    14101 attgaaccat catcgggaac gattttagta gatggaatga aattgacgga aaatcgattg
    14161 agtatcaaac aaaaaatcgg ttatgtgcct gattccccag atattttttt acaattgacg
    14221 gctggagaat attgggactt gatttcagct gcctatgaat tagacacaca aaaaaaagaa
    14281 aagcgtttag cagaattgac agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc
    14341 gcaagttttt cacatggaat gcgtcaaaaa acgattttga tcggcacgtt gctgccagat
    14401 ccagatattt gggtattgga tgaacctttg caaggacttg atcctcaagc agcatttgat
    14461 ttgaaagaaa tgatgaaggc tcatgccgca aaaggaaaaa cagtcatttt ttctacacat
    14521 gtattggata ccgcacaaca gttatgcgat aaactggcta ttttgaagaa aggtgaattg
    14581 atttatcagg gggcagttag tgaattattg aatggctcac ctgatgaaac gctagaaaag
    14641 atttatttga aaatggctgg aaggcaagcg acaggagatg gaccgtatgt ataagcgaca
    14701 attattagaa ttaattcgag ttaatctttt atatgttaat ccacagctca cgaataaagc
    14761 aagagagaaa ggaaaaaagg gaaagcgatt aactatctat cttttgtctc aaaatctttt
    14821 ttcagggctg atttttttac tgatttacgg atttgctatg tttgctatga actttgttaa
    14881 attgccaggt ttctttactt attacgtggc actttttggt atgttaggtt taactcaaag
    14941 tatatcagtc atttataata tcttttttga aggaaatgac ctgcaagtat ttttgcctct
    15001 gccttttgga caagaacagg ttttttcagc aaagattttg gtagttgctt tgaccatcat
    15061 tccttttgtt tttccgcttc tagtcatgtt tattttaacc ggatggcgag ctgatctagg
    15121 gattttgctt actatcatta tctcaatcct tttgttttct ctatttttga ttattctttt
    15181 ttgtctggct agtttgattg tttttggttt ggctcgaaca gcagtgttta aaaaacatag
    15241 aaaaacagtt tctagtatct tgcttggcag ctcgatgctg atcgcatttg ctggaatcat
    15301 gtggatgaat catcaaacgg ggactattga aaatgagtta acagatcggc atccgatttc
    15361 gatattattg ccattctatg atgctgccag tcagccactt tctcaacaag gtttattggg
    15421 ttggggaata ctagctatca cggctctggg atttttcctt gctgtaaaac agctgatagt
    15481 accaaagctt acagagcaac tgactgattc aaacagtgag ttcaaaataa aaagaaccca
    15541 taaaaagaac caaaatgtgc atcagctttt atttagttac aatgctcaat tagtaaaaga
    15601 acctaatttg attatgcaag ttctttcttc ttccttgtta actccaatca tttttattct
    15661 tgcttttgcc ctaggaggcg aaataaaatt aactgattta gataataagt taatcggtgt
    15721 tgttttcctg gtagggatcg cattggcttt cttaacagtt aatcagactt cttttatcag
    15781 caatcttatt tctttggatc aagaaaattt tctgtttatc cgttctttgc caatctctat
    15841 gaaccaatat ttaaaagaaa agttcagatt cggatggata ttgcagagtt ttttaactgg
    15901 tgggatcgca ttattaagcg gactgtcttt tcagctgcct ttgtttttta tccctagctt
    15961 gctatttggt tctttattcg gctgctactt attgtcacta cgttatttcg ctagagatta
    16021 tcggttgctg ctgctgaact ggaccaatat taaccaatta tttaacagag gttcaggaag
    16081 tttgggttta gtcgcaacta tgatgggttc attgatcatc agtattattc ttatcgtctt
    16141 atacggaatg ttggctttgt tttatccatt ttggttagtg aatattcctg tgatcggttt
    16201 agttctaata ctttccgctc tatggatcgt ttattatcaa cgaacgtttt ggaaaaaatt
    16261 tgaataaaga agaggggccg tgacaaaaat catgtcgcag cccctttttt acagatagag
    16321 ggtgttcaaa agtcagtttc tcagtattaa gtcaatttta tgtaaaaatg tgagaagcta
    16381 tttttacaaa acttttttta atactcataa ttacttctta ttgctcctgc ggttacttgt
    16441 cgcaatgttt gtgagatatt ttgtcatcgc tcaaggctga acgtctcttt cacaaccttt
    16501 tctttgttac acttcgttta tttaaaaagc cgtttttttc gtttcatcat gattgcgtca
    16561 cgctttactt tgaaattacg gatgttatgg ttaaagactt ctgataatac aagacctaaa
    16621 gcgattgctc cagcaatcat aattgcttgg accgcataat gagccgcttc tcgataggat
    16681 tcagtaacga gatttcgaac cgcttgataa gctaaaccac cagggactag aggaaccatc
    16741 ccgggaatat tgaaaatcgt tacgggcatt ttcaaacgtt tagagaaaag atcgctgatg
    16801 aatgctactc ctaaagaacc aagcaatgaa ccaaaagcag cagatccgcc tatttgaaca
    16861 cagatccagt agatcatcca gccaaaagca ccagaaagac cacaacaaat caatgaacgc
    16921 ctaggtacat ttgtaataac ggcaaatgcc gcagtcgcta aaaaactaaa agaaaactga
    16981 ataaatatcg tccccatact tgtgttcctt tcctaataaa aaagctggaa aataaaagca
    17041 atcgcaaaac cgatcatcgt agcagtcatc atcgcttctg ttccgcgaga aactcctgag
    17101 acataatgtc cagctagcaa atcacggata gcgttcgtaa tttgaactcc aggaactaaa
    17161 ggcatcacac atccgataat gattagatcc tgattgcttc ctaagccaaa acgtacgctg
    17221 tagatagcgg ctatgccaat caataaagaa gataaaaatt ccgataaaaa tttgattcta
    17281 aaaaatttca aactatatag ataagctgcg tatcccaatc ctccaatgac taaagtcaca
    17341 ggtaaatcgg tcatctttcc gccaaataac accataattg ttccactgat cactgccgcg
    17401 ctaacgattt gcaaccagac aggaaagaaa gaacggtctc ggtcgattgc ttgcagttcg
    17461 cagtaaagtt cttctaatgt tagctctgaa gcaacatatt tacgtgaaag atcatttact
    17521 tttactactt tttctaaatt aatcgaacgg ttggtaattt gttccattcg gatggtcgag
    17581 gtacgatcaa gaccaataaa gagaccggtt tgagtgacat agctgaccaa acggaaattt
    17641 ccagaagcta aagcaattcg actcatcgta tcttctaccc gatacatctc cgcattgctt
    17701 tccatcatga ttttcccagc gagtaaacaa gtgtccagca acagatctaa ttcttttccc
    17761 gtcatagcat cttctccaat aattgattga ttagttttat tttactacaa aacataccag
    17821 aagcgtaaag aataaaaaaa cttgaggaag gaataaaaga ggcgtatact gtttttgtat
    17881 tgcgggtgta gtttagtggt aaaatcccag cttcccaagc tgatgtcgcg ggttcgattc
    17941 ccgtcacccg cttaaaatag gttgtgaaaa agctgctctg caacaagtaa taagaacagc
    18001 caagcaaaaa caacttctta tgtttttaac ttggatggac ttattaccgc agatgcaagc
    18061 ttttgcgcac cgtttaccaa gcggttgtga aatcagtgta ttgtcttgag tatgaagaaa
    18121 atttttgaat aataataaat accttacaga tagagtggtg actgattaga gacgaggaat
    18181 ccgtctcttt tttgtatttt taagccatct taaaacaggt tttatttttt gctgtttcta
    18241 tttctttcta gtatttattt ataggggaac atatgttaag atttagggag ctgttagtaa
    18301 aggagtaaag aaatgagcaa aaaaacaacc aaggcgatcg tggggatcgt cgctgctgtg
    18361 attgcagggg cattcggtgt ttccaccaca caaaaagatt cagatatctt tcagcagatt
    18421 acaactattt ttgattccac accacaagct tctcaagtag cggatgtgcc agaatatgat
    18481 ggaaaacatc aagaaataga aatcaatggg aatcagccgg aatttacaaa agaggatctt
    18541 agcctttcca aaggaacatg ggaaagctat tcaaatattg ataaattgaa cagagtagga
    18601 caagccaatg ctatgcttgg aaaagatttg ttccctactg aaaagagaga gcctttgtat
    18661 attgacccga caggttggaa acaaaagaaa ttaagtgacg ggcaatggtt atataatcgc
    18721 agccatttaa ttggtttcca gctgactggt caaaataaca acattaaaaa cttgatgaca
    18781 gggacacgtt cgttgaatgc tccatacatg cttgctcacg aaaatgatat tgcggcatat
    18841 atcaaagaaa caaatcatca tgtacgctac cgtgtcactc ctcattttga ggggaatgaa
    18901 ctggtagcca ggggagtgca attggaagca gaaagcattg aagacaaaaa aattgaattc
    18961 aatgtcttta tctacaatgt tcaagatggc tacacgatca attatgagac aggacaagca
    19021 acaaaaacaa aaaattagta gcagctattc gaatttttta tgaatattat gaaaaaattg
    19081 tgtaaaaaca tacattcaaa gaaatatagc gtatacttta tgaaaagagt acttttgata
    19141 aagctacaga aaaaaggaaa aaaacataca aatagtgttt cttaatttat tcggaagttt
    19201 ggaatatttg tttgtagttt tccctttctt cgagtaaaat aaggttgtag aaggtaagct
    19261 ggaattacct tgcgggaaag aggtgtagaa tcatgggttt tacagatgaa accgtacgtt
    19321 ttggttttga cgatagtcgc aaaaaagaag tgagcgaaac attagcgatt gtttatcggg
    19381 ctttggaaga aaaaggctac aatccaatca atcagattgt cggctacttg ctttctggag
    19441 acccgtccta cattcctcgt tatcgtgatg ctcgaaattt gattcgccgt cacgaacgcg
    19501 atgagatcat ggaagtaatt gtcaaggact acctctcaaa tcatggagtg aatttatgag
    19561 agtaatgggc ttggatgtcg gttctaagac agtaggtgtt gctatcagtg acccctttgg
    19621 ctggacagct caaggagtag agatcattcg gatcaatgaa gcagaagaag agtttggctt
    19681 tgatcggttg aaggaattag tagcacaata tgaagtagac cggtttgtag ttggattacc
    19741 taaaaatatg aacaattcca tcggaccaag ggcagaagct tccatgtcat acggagaaaa
    19801 gatcaaagaa ctattctcct tgccagttga ttatcaagat gaacgattaa ccactgttca
    19861 ggcagaacgt atgctagtag agcaggcaaa tacttcaaga gccaaacgaa aaaaagtcat
    19921 cgataaatta gcagctgtga tgatcttaca aaattattta gacggctcag gtaagcaatt
    19981 attttaataa taaaagaaaa gaggtaatct tatgacagaa cataaccatt cccacgacca
    20041 tgaaggacac gaacacatca cattggtgga tgaccaagga aatgaaacat tatatgaaat
    20101 cctactaaca gttgacggac aagaagaatt tggacgtaac tatgttcttt tatatcctgc
    20161 tggtgtttca gaagatgaag atgtagaatt acaagcttat gcatacatcg aaaacgaaga
    20221 tggaacagaa ggcgagttag aacaaatcga aacagacgca gaatgggaca tgattgaaga
    20281 agtattcaat acattcatgg cggaagaaga agaatagact ataaagctag gagaaacctt
    20341 gatatgatag ggtttctcct ttttttattt tttactgtat gccatattgt atgccatcta
    20401 gtcaatattt aagtgttgta ctaatttttc aacagtcttt tctttcgttt cttttgccaa
    20461 gtgaccgtaa caatcgatcg tctgactaat ttttgtatgc cgtaatctag tttgaacttc
    20521 taccatagta gctccagcgt ccaagagcat tgaagcatga gtgtgtcttg ctacatgcac
    20581 accgagaaat ggaaccatag ctttttcaca aatagtaatc aatcttgcat aaatagcgtg
    20641 gtgtgtgatt agttctttat agataccaca aaaaatcata gtcggatcag ttacaccaag
    20701 tgtcagcata tatttccgct gattaatctg ccattttttt aatatagaaa ttgttctttc
    20761 atctagtgga agaaccccag cactacaatc agttttagga tcactgattt tatagccatt
    20821 tttggattgg cttagtgttt tattgatggt gaccgtattt tcttgaaaat caatgtctga
    20881 ccaattcaat gcaagggctt cactgatcct aaccccgcta taaaagagaa agcgtagtaa
    20941 cgttttatca aagtcgtttc gataagtttc aggtttgctg tttaggtaat caaacaaggt
    21001 gatcagttgt tctttactgt aaactttgac cttttcttta tgcttagtgg tggttctttt
    21061 aggcattaat acgttgtcta gtggattagt ctcaataatc ccaagattga tagcgtagct
    21121 aaaaatacgc ttagaagttg aatgaagtac cttgtattgg tcagtagttt cagcccagct
    21181 atttacaatt tgttgggctt tttttaccgt gacttcattc acacgaagat ttccatattt
    21241 tggaagaatc cactttctaa acttgatttc agttttcatg aaggtggctt ctcggacagt
    21301 ggttttatat gtttctagcc acaagtcata aacttcttta aacgtttcgt tgttcgcttt
    21361 tttaataccg tttttatcgt agtccacttg caagcgtgac aaggcaagct gggcttcttt
    21421 ttttgttttg taacctcgtc tacgaacctt atcaggtttg ccagtcattt tgttcattcc
    21481 aagataggct tcatagagcc aaaatttacc ttttttagtt tcatattgct tgaatgttgc
    21541 cataatagtt ttccctccat agtgtagcaa gccgtggggg cggttggagg aggcagttta
    21601 ttattatttt gaatagtgtt tatttagctc agtaacaaga gctgtgaact cttttagaag
    21661 atcattttca taaaattctt gatactcttt tttctcatct gtattttttt ctgaatcatt
    21721 aattaatgag tcatatcgta aaagcgtatt tagaagagct gcgagataag caattggggt
    21781 actcccgtca ggaaattcaa ttcgagatcc tgttttaaga tattttattg cgtttgttaa
    21841 ataaatcttc tgtgagacag tcaggttatc ttcttcgatg ttattgatag cattagaaag
    21901 attagaatca attatagagt tatttttttc aatagttttt ttcaattgct tttcaagcac
    21961 atttcgctta atttcgggca tcatatgcat atctcttaat gtataagatc cgtttaatag
    22021 ataggtagtg gatacatcta atacctttga aagttgttta agcctggtgt tattgggaag
    22081 attatagcca ttttcccaat tactaacggc acctttactg gccggtgggg ataatttacc
    22141 tccaagttgt tccattgaga gtcccttttc aattctagcc agtctaattc tttgtcctgt
    22201 ctccactgct tcggggtatt tttttctagc cacattttca ccaccattca tatattttct
    22261 ctactatatc acgatttaaa tatgtgtgaa acataaaagt tcaaaaaagt atgttaaaat
    22321 tcttgcaaat gaaaaggtat gatggtatat tgagtttata agaggtatag aaatgtattc
    22381 aagagcctct tgcatttttt taaaatcttt gtatagaaat gtatgctaac gaggggggat
    22441 aaatatggaa ttaaaaattg acaaactcac gcttgatgat ccagctttta aaattattga
    22501 cgatcgagtt gttcaacaag tagagcaaac tcaaggaacg ttactttcat tattagaaaa
    22561 aaagtaccag tttccaaaat atatgaacaa ggcacaagct gctaagtata tgaacgtttc
    22621 atacaatact atgatgcaga aatatgttcc taacggttta aagttgatta tagttgatgg
    22681 agtcgtaaga atttcacaag atgaatgtga tcgctttatg gaagaatacc aaaaataaac
    22741 gcaagccgtg ggggcaaagg gattaatagg aggaaataat aatggaatcg acacaaaata
    22801 aagcaatcga aaaagtatta tcaacaattc ttacagaaga cacagcggca gaattagcaa
    22861 acttggaagg caaagcgcta gaagaaacgt ttgaatgttt gtacgaacaa atggactatc
    22921 aaaaattgct accacaagaa ccaacggtaa gcggagtatt gcgtggcttg aacgatttag
    22981 tacaagcaga gtttaaagaa cgactttcta ttgaagagta tcaagaaatt ttgtatcagc
    23041 aagttgagtt gttagcgagc ttgctagggc ttgaactgga ggacgttgaa tgaaaactga
    23101 aatttggaac ggacacatta taagatttgt cgatattaat gatgaatggt gggcagtggc
    23161 gaaagatgtt gcggaagcgt taggtcttaa acaggttact agagccattc actctttgcc
    23221 taaagatggg gttactacta gtaaggtcat cgattcattg ggcagaacac aagatgtaaa
    23281 catcatcaac gaaaaaaaat atttaccgca tggcattcaa aagccgtaaa aaagaagccg
    23341 aggcatttca agactggatt ttcgacatca tcaaagaact acgccagtcc acaggacttg
    23401 aaggctttca agtattccgc atgctagaca aagaacacca aaaagaagca atgactaaat
    23461 taagccacgc tattactgaa ccaaaaccag tagactacat taaagcgaat gtgatagcca
    23521 ataaagctgt atcaactatt tatggtcatt ccaaaatggt aaaaaagaaa gatatgactc
    23581 ctgaaatgtt ggttgatcgt gaacctattt tagatgagac agtagaatta atgactgtta
    23641 aagaaaaata tgggttgcag ttttcagtga gtgagaaaat ttataatcgt tctgctgaat
    23701 tgcagacaac ttaggagcat taacatgatc gagaacaaag aaaaagcctt atccataaca
    23761 gtcttggcgg acaggataaa ggcaacttaa ttgggataaa acccgatatt ctttgttccg
    23821 attataccat attaaaaaat cggagggaac cattatgaaa cgaaacttaa ttataggaag
    23881 cattttaatc ggcggaattt tcggcttatt accattaatt gcaaaaatag ctatctttgc
    23941 aggattagcg ttatatctct tcactgctta tgacgagtac gactatcaat taagaactgg
    24001 agggcataag tagatgtaca aaacaaaact attaaaccaa ctcgatagcc ttgaattaga
    24061 ggaaattaat caagggatcg ctgaactaga aaataatatc gggaaaacct actttggaaa
    24121 ttcattcaat gaaaaactaa cagttttgta tgtactcaaa aaacatgcag aacacaaaat
    24181 aatttgtcga gaaatcaacg agctaaaaaa tcagatttta accgcatggt taaacattac
    24241 tgatatgcaa gaagcaaggg tgaaaacatt taatacatgg gtaaagtatc agaatcaatt
    24301 aaaaggtgct gaatttgttc gggatgggtt gaaatacgaa cttgagcagt tgaagctcat
    24361 ggaggtgtct gaatgattta taccagtatt ttgaaaaagg aggagaggtt tattggcaaa
    24421 taattatcaa gcagcaacgg aattacaaaa gtcgggatgg tcaatttatc ccttatctcc
    24481 tggaactaaa attccaataa aaggctctag tggttggaat gatgcaacta acgattctaa
    24541 tcaaattaat gaatggtgga cggaagatgc aactagaaat attgggttaa tgcttgaaga
    24601 tcatcaaatg gtggtagttg acctagatca acattctgaa acacgaaacg gcgttgagaa
    24661 ttataaaaaa cttgcttcaa tgtatgatcc atttccttct acttatacgg aaatcacacc
    24721 aggtaaagga atccatttct tctttaaaat tcctgatggt gtaattatcc aacaagaaac
    24781 aggtgcattt gctgatatct tcgggcgtga tgataaaggt aaaagtttaa gcgggattga
    24841 tattatcact aaaggagttc caatagcgcc aactatgatt atttcaagaa gtgttgggag
    24901 agcatatgaa cctattgaaa acgtatctct aaataatatt gcaccagctc ccgattggct
    24961 agtgaaccta ctgctaaaaa aaccgcaaga aataaagact caataccgac ccgtagcaaa
    25021 aacagggaca gcaaaaaaat tagatatgat tgttcaaggc gctggcgaag gtagtagaaa
    25081 cgatcattta actaagttat gcggttggtt actatggcac ggtgtagata atcaaacttt
    25141 agcagaattg atttatacag caaatgcgta caatgaacca ccgctaaaag ataaagaagt
    25201 gaatcaaatt attcgatcga tgattaaaaa agatttgagg gggaaagctc atggctcata
    25261 agcataaatt agatagtatt ttagactttc ccgaagccag cgagcgtgaa gacaatatca
    25321 ttgaattgaa aacctggatg tcacgattaa gatgcaataa agacgatcag attaaaagta
    25381 acagtgttgt gaatgcagaa ttgattttaa ccaatgatag caatttagct ggaacaattg
    25441 cttataacga atttagcgga tacattcacc tattaaaaga ttctccttgg atcaatcgta
    25501 ccgctggaga atgggaagac agttttgagg atgccttaac ggcatatatt gaagaaaact
    25561 acaatgtagt ttttgatgac aacaaaatac acaaagcagt agtaaatgtg gctagaaaga
    25621 atgttttcaa cccagtaaaa gaacgtatcg aaaaagttaa atgggatcaa cagcctagac
    25681 tggaaacact gtttattgac cttttagggg tagacgacaa cctttacaca cgagaagtaa
    25741 caaaacgttg gatagtcgga tcagtcgcac gtatctataa accaggaata aagtttgaga
    25801 tcgttccagt tttggatgga ccgcaaggga ttggtaagag taccgttcca gcattacttt
    25861 acacggatga ttttttcacc gattcattag attcgctagg tgaaaagaaa gatgattata
    25921 tgcagcttca aggcaatgta attattgaac ttggagaatt atcttcaatg aacaaaacta
    25981 aaattgaaca gataaaaaac ttcatcagtg ccaagatcga taaaattaga cctccttatg
    26041 gtcggaatgt catagcttgg gcaaggcaat gtgtatttat tggaacgtcg aatgatgggc
    26101 agtatttgaa agatgataca ggaaaccgcc gtttttatcc tttgccttgt aagaataagc
    26161 caaagatgaa tccgtttaaa acaaaggatg aatattttct tcaagtgtta gctgaagcaa
    26221 aggtgctttt tgataaagga caacgaatct attttagtcc cgatgaagat aaagaagtct
    26281 tagaaattgc aaaagactat caagaggatg ccaagatgga gaatccaatt aaagaagcaa
    26341 tcactaaata tttggaaatg gaaatcccgt ataaatggga agaagctcca gcgtgggcga
    26401 gacgttctta ttatcagaac tatcctgaca gtaaatgtaa tgagaaaaat ttacagcatt
    26461 tctataatgg agctactatg caacaagagt tttatttagt tgatagcgta ctcaccgctg
    26521 atattttgga agctgttttt gataaacaag caaaggattt attgaatggt cgatcagatg
    26581 cagaaactaa aaagattgcc ttgattatta gcagtattcc tggttgggaa cggaagcaac
    26641 tgccaaaaag aaataaacga cgaggatttt acaataagtc aaatgcctat gaaaataaaa
    26701 atcgagctgg aaagtacaaa aagtaacggg tagtaacggg tcagtaacac ctagcccgtt
    26761 actagtttac ccttactgcc ccaagggata cagcaatttg gtaacggggt aacaccttat
    26821 ttccttaaaa cttttgaaat agttagctaa tatgctgggg aataatgtta ctggatttta
    26881 cccgttacct gttaccaaaa cgctagaacc cttgtccccg ttgggattga gtggtaacgg
    26941 gtacctgtta cgtaggtgtt accacgtgtt acgaatcaag gaggaaagta aatgctcaat
    27001 attatttcaa caaacaaagc accgaacttt caatatacag atgaaatgga tcggtttcta
    27061 atgaacacgc tggcatttag tgtgggattg gtaacagaag attattccac atttgatcca
    27121 gaagtattaa agataatgga agaagaacct gattggctgc aggagtcagt agcgtggtgt
    27181 caatcgctag tagtgggatc attggtagat agtggcaact atgatgatac aggtgagcta
    27241 atggatgaat ttaattgtct gcttaaccta tatgatcgag cgagacaacg agagcttaca
    27301 tcgaatgagg ataatttgtt tttaaacatc catgataagt tcttggcttt gctactaaca
    27361 gatgatgaat tgataactaa tttattggag gtagaataat tatgcatatg gaaagtatta
    27421 gatacttcga tggtgagaaa cacattgagt attcaaacac tcaagaagat gtagacttta
    27481 ttagttttga ggaagataaa atcactaaag ttaattttaa agacggaacg tgcttgaaga
    27541 tagtttcgcc ttatattgaa tacaaatcaa aatgggaaca agatgattct gacgaacgag
    27601 atcgctttgg ttggtaatct atggcagtta aaaaacaatg taatcatgct ggatgtaaaa
    27661 cattgattga ttataggcag aagtattgtg ggaagcataa agcaaagcaa acggcaatta
    27721 agcgtgaaga aagaaagcaa agcgaaggta aatattttca attctatcaa agtagaacat
    27781 ggcgaaaggc ttcatacttg tatcggttaa atcatccagt atgtgaggat tgcctagaag
    27841 aaggcttgat aaggaaagct gatgttgtag atcacaaaat tgaactaaaa gatgattgga
    27901 atagacggtt agatgaaact aattttcgtt ctttatgcca tgcacaccat aattccaaga
    27961 cagccaatga aagacaaaga cgagaaaaga gtaccctttg agttaagggg gcatagtgtg
    28021 aaacttactg acaatcgatg cctactcatt ttggtacaaa taaccgttgt gaaaaggcat
    28081 aagggtaatt acaaataacg attacacttg taatcacaag tgaaaaagta tataatgaaa
    28141 gtaggagatt tattgaaaaa tacaattgtt attactgata aaacagggaa acagcgcacc
    28201 attgagttac ctcattttgg tcaagtgacc attcaaatgc agaacggaaa aatcatttat
    28261 atagataaat tagataaaga aaaattctga tcgaaaaacg aaggaatgtg agcttaattg
    28321 cttacgttcc tttttctttt gtctgaaagg aggacaacat gggacaccct aaaatattac
    28381 acgatacaaa aggcaatata tcaagtgaag aaaaagcaat ccgtgtggac gctagagaag
    28441 aattgttcaa gcagcaacca ttaataaata tcacgccccc tgactggatg gcagcgagcg
    28501 ctagaagtga atggaatcgt atcgtaccaa cattaaaaaa agattatcca ttgagtgaag
    28561 cagattatgg ttcattggta gcctattgtt tagcctttgc tcgaatgaaa acagccgaag
    28621 ccgagataag aaaatcaggt acgtttatca catgcgaaaa cggagtaaag aaagccaatc
    28681 cagcggttcg ggttcaatca caagctatga gtgatttgaa aaaacaagcc acctcactag
    28741 gtatgacctt agaatcacga tcaaaactag ctttgaacaa ggttaaaaat gatgaacccg
    28801 aagacccgtt caaagagttg atgaattcat gaatgattac attgagaaag tcctatcagg
    28861 taagctgatt gcacccaaga aaattatcca agcgtgtgag cgtcatataa gcgatttgga
    28921 gcgttctaag tcagatagct tcccttatgt gttcgatgaa gaacaagcca ccaaagcgat
    28981 taaattcatt gagttgctac catctacgga cggtaaagca atcaagatgt taggatttca
    29041 aaaattcatt ctaggcagtc tttatggctg gcgtactaaa gaagggaatt acaggcgatt
    29101 caatcgagcg tttaccagta tgagccgtaa gaatgggaaa acgtatatcg caagtggcat
    29161 ggctgccaat gcgttgatta tggaacaaga accagcagaa gcaaggcaag tattgtttgt
    29221 aagtaacgct ttgaaacaag ctaaattggg ctatgatatg ctgtctaatt cacttagaaa
    29281 cgtggtcaag tctagtaagt ttttaagacc acaactgaaa attatgaact ctaagattca
    29341 gcacttgccc tctaattcgt tcgctatggc actggctagt gaaaccagca cgctagatgg
    29401 gtttgcacca acaaccgcaa ttcttgacga gtggcacgaa gcaaaaactc gtaaaacgta
    29461 caacgtcatt aagtcaggaa tgacccaaca aaagaacggc ttattgtgtg ttattagtac
    29521 cgctgggctt gatttaaacg ttcctatgta cgaggaatac ttattgttag atcgtgtgct
    29581 aaaaggcgaa gaacaagccg acaggtactt tatagcgata tgggaattgg acgatcccga
    29641 agaaattcac gatcaagaga aatggatcaa agccaatccg atttttgaaa gtgaagaaat
    29701 caaaaaagta atgattccaa ccattcaaga tgatgtgaac cttgctttga aacaaaataa
    29761 ccttaattct gtattggtga aaaacttcaa tttatggaga caagcgagtg aggacagcta
    29821 tatgattgct gaagactggc aggcaaccga agtagaacca caagatatta caggcaagcc
    29881 cgtttatatc ggagtggatt tatctaaaac agatgattta actagcgttt catggatcgt
    29941 accgctagat aacggcgaac tttattgtga ttcacatagc tttgtagcca ccaaatatgg
    30001 gcttcaagac aaagaaaagc gcgacggttt gccttatcga gaactagaaa aagctggtga
    30061 gtgttccatt actcaattag aaagtggaat cgtggactat gaccaagtat ttcagtttat
    30121 tcaagatttg attcaagaaa atgatttgga atgtatgggg atttgttatg acccgtataa
    30181 cgctaattcg cttatcagta aagctgaaaa agccaactac ccaatgttag aagtaagaca
    30241 aggaacgatt actctaaacg ttccgacccg aacttttaga gaacaagttt atgaaggcaa
    30301 cgttattcac aataaaaata cgattctcac ccatgcagtg aacaacgcta ttttaaaaac
    30361 ggataacaac ggcattcaga ttaataaatc aaagaacagt aacaaaattg atccaatagc
    30421 tgcattaatc aatgcctatg tgtttgcaat ggattacttc accacaacgg aaggagcgaa
    30481 agcagacaat gaattttata caagtgaaga attttctttc taattacatt cataccgttc
    30541 ttttacttct tggattggtg tgtgtgttgg ttgctatcac cttactaaca aatgtctact
    30601 atggcttgtt agcgctgggc atagtgctta ttggggtagc ggtcatgcta aatacagaac
    30661 agaagggagg ttaaaagatg gcatttttta aagcgagaca aaatacaacg ggagatcctt
    30721 tcctacaaca tgtggtatca atccaatcgg atgattacac caccagcttt acaagcgttc
    30781 gtgcattaag aaatagtgat gtgtttgcag ccgttcgaat tattgctagt gatattgctt
    30841 caagtccgat tcaattggtc aaaaacaata tgccacaagc tgataatgaa ctggtgaagt
    30901 tactaaacga gaaacctaat tcagaaatgg atggttggca tttaaagttt gctttggcgg
    30961 tcaatatgtt gctaaacggt aatagctttg cagagattaa acgcaacggt gaaaaggtag
    31021 aagaaattca cttattacct aactcaagtg taacggttac tcaattagat aatggtacgt
    31081 tgtcttatca gattggtgat aaaaaaagac gtgtgaagtc tagcgatatt ttgcacttca
    31141 aatatttcac ccaagatggt ttaacaggat taccaccact ttatgcgtta cgtgatgaac
    31201 tgaaaattca acaagctggc aaccgcacat tgcacaattt ctttactcgt ggtgtcagtg
    31261 ggtcaggtat tttgaaggtt cataagtcag atttagacgg atcagcaaag aacgcaattc
    31321 gtgaaaagtt tgaagaagcc aacggttcaa gtagtggaga taatgctctt agaacgatca
    31381 ttcttgatga aacaatggac tacaaaacat tagaaataaa caccgatgtt ttgaagctta
    31441 tcaattccaa tgattggaac acaaaacaaa ttgccaaggc gtttggtgta ccgattgagc
    31501 gtttaggcgt tgaaaatgaa cattctagca ccgttcaaag taatctccag tatatccaaa
    31561 gtacgctgat tcattacttt aatgtgtttg tgagtgaatt agatacgaaa cttgaaacca
    31621 atatccgctt taattccgat cagttactag agactgatcc tgaaacaaaa gtaaaaaatg
    31681 tattggatca ggtcaaaggg tcacttctca cgattaatga ggggcggtcg aaaatggggc
    31741 taccccccat ggatggtggc gatcgtttac tagcaagttt gaactttacg tatttagata
    31801 cgttggagaa atatcaatta aaagaacagg aaggagttac accagttgaa taatgaagaa
    31861 gaaaaggaaa aacggctgac agaagaagct gagctaacag ccgattctcc caaagtagag
    31921 aaagaaaatg aaaaacaacc aacagacggc aaaactattt caggctatgc gttgaaattc
    31981 gggcaaccgt caaaagattt aggcggcttt gtagaagtga ttacacccga agcattaaaa
    32041 gaggtggatt tatcaaatgt gttcttatta tatggacacg attattcaaa gccactagct
    32101 agtgtcaaag caggtacgtt gaaattgaat gtcgatgata caggattaca ttttgaagca
    32161 gagctaaccg acacgaccta ttcaaatgat gtgtatgaga atatctcaaa aggggttgtt
    32221 gattctatgt cctttgggtt cgtgcttgga ttagattcat tcgacaaaaa agaagatggc
    32281 acaattgaac gatcaataga taaaatcaaa gcacttaatg aaattagtgt cgtgaccgtt
    32341 cccgcttatg attcatcaaa tgtccaagta aataagcgtt cttacgaatc gtttatgagt
    32401 aacaaccaag caaaacaaac aaacaatagc ttagaatcca cttctaaagc acaaaaggag
    32461 agtaataaca tggaaaaaac tttaatcgat aacgaaaaaa cagaattgcg tgggtatgaa
    32521 gaatatatcc gttcacaagg cgaagtgcgt gatggagtca ctactgtaaa tgcagcggca
    32581 gttgttcccg aagaagtaat cggtgaagtc tttgatttga aacgttcaaa ttataactta
    32641 gctcaatatg caacagtaaa aacagtatca aatggacaag gtaaatatcc agtagcaact
    32701 aaccaacaag cagtgttagc aacaaaagct gaacttgctg aaattggtga tattgacgct
    32761 gaaatgttta cttcagttga ttataaagta gaaactcgtg ctggtaagat tgccttatca
    32821 aatgaggttg tggaagattc agcagtgaat attgtacaag aggtcaaaga tcagttagcg
    32881 aaattggtag aaaacactga caataagcat atcatggatt tattaaaaac attcactaag
    32941 aaaacggctg ctacgttgga cgatttgaaa caactataca atgtggcatt agacccagca
    33001 ttaaataaaa tggtaattct aaaccaaagc ggatattacc acctagatac attgaaagat
    33061 tcagatggac gttacatttt acaacccgat gtgacagcac ctagtggtaa atcattattc
    33121 ggtatgccag tagtattgat tgcagataca ttgtttgcca atcctaaagc tggaacattc
    33181 cctatgatta tgggggatat tgcacaatct atctttgttg ctcgtagaaa tcaagtaacg
    33241 actcaatggg aaaaattcga ttactactca caaggacttg cagtgatcgt tcgcaacgat
    33301 tataagaaaa ttgatcaaaa tgcttcagtg tatattgagt ttacgccagt tgtaacacca
    33361 aaagtataga aaaatattgg gcggcggttt atcccactgc ctttttttca ttaggagaga
    33421 taaaatggta attttggata gcataaaaaa aagtatgcgg atcgatcaca ctattgatga
    33481 tgactttatt caacaattga ttgatacagc aggcgaatat ataaaaagtg ctattgatag
    33541 tagcgcaacg gataaagata tggataatta tcagcaattt gatttggcgg tgtcattact
    33601 tactcaacat tggtatttga accgtcaaga agccagcagc gaacggatac cagtaacggt
    33661 acaagcatta gtacaacaaa tgagaggtgc ttattatgcc aatcattaag aatgtaaatg
    33721 aattgactga gagagtcaag tttaaaaaga caaaacgggt aaaagatgaa gatgggcaga
    33781 tggtggatag tgaagaaact gtatttgaat gttgggctaa tgtgcgttca caaatgctaa
    33841 aagacgtgct tgctagtgta ggtaccattc ttgaaggaac gttgacgttt attattcgat
    33901 acgatcaaga ttatgaacta acaaacgata tgaaagtagc ttggaaaaac aagagttata
    33961 aaattatttc aatcaatgaa ggaacggcgt ttaaagatta tacaacgatc atagctaaac
    34021 agatttctta gaatgattac acttgtaaac gttgtgggta tttagtataa ttaaggtagt
    34081 aaatgaaggg ttagctgcct gaattttgca agacttagct agtcgaaatc tattgttgga
    34141 ctgaaaattg taggtgtggt tgcaaacatg ctggactaag aaagatgata gatgatgaac
    34201 gttcaatttt aatacaggca tgctaggact gattggatgg ttttggcaag ctgattgtgt
    34261 agaaatcaaa gaacacgtct gttgtggacg tgtttttttt gttataattc aaaagaggtg
    34321 atttaatgga caaagcagag tttacaaatt tttattggaa gtattatcta catttagaaa
    34381 atgagtttat taataccaca gattttgtga tgctcgatga gaataatttc aaaacattct
    34441 ctattgaata tcaaaaacta ctactagcga ttggttcgga gtgtgagatt attttcaaag
    34501 aactttgcgg atttgatagc agtagcaata aaagcataac tgattttaaa gctgttatta
    34561 aatctagcga attaatgtgt ttggataatg gtgttagggt tatgaattct ttaacattga
    34621 cttcactaaa accttttgga gatgggtggc ctgaagatac accagattgg tggaaaatat
    34681 ataacagagt aaaacatggt cgttcttcaa attataagga agcaaatttg gaaaatgtgt
    34741 tgtatgcttt agcaagtcta tatttattag aggaatattt gcataagaaa gtaatactag
    34801 aaggtgaagt agactttata agtccagagt caaatctatt tacgttatct tgggaaagaa
    34861 aacattcgtc gatgcaaaaa gtgacgtttg aaaaagtgga ttcaaattat gtagctccac
    34921 tcattgattt taaagaacaa gaagaaattt aaaaaccccc acggcttatt gaagtttttg
    34981 tatgccatat tgtatgccat ttggtttaaa tcgcttgaaa aatagtgata gataactcta
    35041 attcatttag tcataaacgc tgttttgata acctttatag ttctttgata tgggtttctc
    35101 attcatggcg gaagaagaag aatagaacaa aagaatgaat taataagata taaaaaaagg
    35161 gattgtgaca aacgtcttgt cacaatccct ttttccaaat aaacgatgtt caatcagctg
    35221 cccctcggca ttcgctcgaa aaacggcgaa atatgaagaa ctattttgcc atttgtttct
    35281 ctaataatca ggtcaaaacg ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc
    35341 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt
    35401 tccttgtgac tcggagctga acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
    35461 gcttgcatct gcggtaataa gttcaaccaa acgaaaatta tgaggagcta tttttgtttg
    35521 gctgttctta ttacttgatg caggacagct ttttcacaac ctcttttcgt ttagcactaa
    35581 atttccttaa aaagtagaaa agctttcgaa gcctcgtttg tttcttttac tgtataataa
    35641 aagaaaagag taacaatcgc taaaagatat aaacaagaga aaggaaatac aagatgattg
    35701 gtctgattta ttttgtggtg atcgttctgg cgaacacagt tggcgctatt tccgggatgg
    35761 gtggaggcgt gttgatcaaa ccgattcttg atcttatcgg cgcccattct gttgcaggga
    35821 tttcttttta ctcaacagtt gcggtattca cgatgtccat tgtttcgact gtgcgacaag
    35881 tatcaagcgg gaaatcactg aattggcaga ttgtcggttg ggtatctagt ggagctgttg
    35941 ttggaggagt agtcggcaac attgtattcg aagttttttt acagttattt gaaaacgaga
    36001 agcatgtgca gctgatccaa atttttttga cggtattgac cttggttttt gcgttcttct
    36061 acaccaagca tcatcagcca aaatttcatt tgacgtcatg gacttggtac cttatatgtg
    36121 ggggggttct gggcttttta gctagttttt taggtattgg tggtggacca gtcaatgttt
    36181 ctttgttgat gctgatgttt gcattgccaa taaaagaagc aacactatat tctctatcta
    36241 cgattttctt ttctcaactg gcaaaattgg taacgattgc tttaacaagt agctttatgc
    36301 gctttgattt atctatgttg ttttatgtta tccccgctgc tgttattgga ggtttgtggg
    36361 gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac ctttattttt caagtaattg
    36421 tcctcgtggt tcttttggtc aattgttaca atgcctttgt catcttacaa acattaggag
    36481 aatctagact aacgcaagtc aaaatcactg aaacatattg ttctaaatga aaaaaggagt
    36541 atactgtgat aaaaaaattc atttaaaaaa ataaaaattg gctggtgatt tggatgttgc
    36601 gggaaatgcg tttaggagat gcagaagcac tacgagaaat caatgcggaa caattaggat
    36661 atgatgtacc attaaccgtt acggtgagac aaatgaagaa actgttgaag gagccagaaa
    36721 aaaatttttt cttggtctat gaagatccta cttctaaaaa agcagctggc tatgtacaag
    36781 cggagtccta tgaatcgatt ttcagcgatc cgatgttcaa tattatggca cttgctgtgt
    36841 ctaaacatgc tgaaaaaaga ggaatcggaa aggccttgat gactggcgta gaagaagcga
    36901 aaagacgagg aatatcagca ataagactta attctgcaga atacagagta gaagcacacc
    36961 gattttatga acgcatcggt tatcattccg ataaaatgca aaaaagattt ttgaaaatcc
    37021 tgtaaatttt tatcaaaaaa tacctggtca tcaagccgat acttgatgat caggtatttt
    37081 ttttgactaa gaaagtaatt gcatcaagaa acctgagatg atcaaaacga tggcaccacc
    37141 aagccgattg cccatttgag caaaagcaat aagttccatc cgattagctg cagataagac
    37201 agctacattt cctgtaccac ccatcgagtt gttgcataag ccagcagtaa tagttgattc
    37261 gattggatat aaaccaaaca aacgaccgac aaatcctgaa actactgaaa tcgcgataac
    37321 acttgtcaaa cataagacaa caaattgcca tgtcaaagca gaagccaata catttagatt
    37381 caataacgca ataccgatac cggcaagaac agctgctgtc aagtttttga caatcaagtt
    37441 gttgaacatg atagctgcat cttcgtaata ttctggtaat aggtttgcga ttttacagat
    37501 aacaacgata atgatcatga atgcgtaagc atggacttta ggaacaaaat agttacagat
    37561 ttgtcctaag atgaagaaag tcaatgatac catcaaaccg acacctaatt gtggaatgga
    37621 aggttttaca tttctaggtt ttccatcgcc aaggtcgcct ttttccatta atttaccatt
    37681 accgttgaat tttggcattg attgtcccgc acgagcaact aacgctgcac caatgatagc
    37741 taggacattt gtcattgctg tcgcaggaat caggcgggaa atgatagaag aagcgtccgt
    37801 acctaaaaca tttgcataaa tcgttgataa aggtacagct ccggctccaa caccaccagc
    37861 cattgcgggc aatgagatat ataaaacgga atttgcaaaa ccatttccga taagcatacc
    37921 tacgagtcct acggcaaaaa aggagacgac cattgaaata agtgcaacgg gaataaaacg
    37981 aacagaagct ttcatcaata gattacggtt cataccaagg atgctaccag taatcaaggc
    38041 agcaatataa aagtctaaaa agcccatatt gctgacaaag ttttctgtgg attttactac
    38101 atactcaggt aagatcccaa atgtagcaat cgcggcagag gcaaagatcg caaaaacgga
    38161 gccaccacct aaataacttt tgacgatagg gatttttgtt ccgataaaat gaaataaatt
    38221 accgaaaatg accaaaacag cgattgggcc aatcatattc gtcggtaatt ttcctaaagc
    38281 cattgcaatg atcaggacaa ggaccatgat caaataaagg ggcaaactta cgccggtaat
    38341 ttttgttgac caaaaccctt cttttttctt ttcctcaact gcagttgcgg ttgttgtttt
    38401 ttgtgccatt tttacagatc ctttcttatt ctgtatcgta tgaaaaaagc caaagagaag
    38461 cggcaaaaga gcagtagttg gctggctgct gttttgccgc agtcgctttt ttctttttag
    38521 acgtcataca aagtttctgt atgttctttt actataacaa tcttaatctt ctaaagattt
    38581 atattcagga acccatttag cagattcaac agcttctttg atatccgtga tttcttcacg
    38641 attcaatttt tgtgcaacaa ctgattttgc gacagtttca gcaatcgttt gagagaattc
    38701 agtgattttt gctactggag gcaagatagc tgctcctggt tttgttacat caacgattcc
    38761 gcctaatgct cgactagctt gagaaatgat ttcagcattt actcgtgtag ctgttgaagc
    38821 aatcaaacca agccctaaac ctgggtacat caacgcatta tttgcttgac cgatttggta
    38881 agttacaccg ttatattcca catcagcagc agggatacct gttccaacta aagcttttcc
    38941 gtccgtccat tcaatcaaat cttttgcttt tgcttctgcc aattttgttg gattagacaa
    39001 aggcatgatg attggacgtg gggtatgagc cgccatttct ttcacgattt cttcagtgaa
    39061 tgctccaggt tgggtagaag taccgatcat gatcgttgga tggattgctt ttacaactgc
    39121 ttctaagttt gtcaattcat cagcattagt gaattctgag cgtttacgag cgaatgggat
    39181 ttgaccagga gtcaatcctt ttgtatcttc aaacaataaa ccctgtttat ctacttgata
    39241 gaaatgttgg cgtgcttctt cttcaggtac accttgacgg atcatttcat ctaaaaggat
    39301 gtttgcgata cctacaccag cagtaccagc accaaatgtc aagatcgttt gatctttcaa
    39361 ttgctcacct gatatattca atccacctaa tacaccagct agtacaacga ttcctgtgcc
    39421 ttggatatca tcattgaaag tagtgatttt atcttcgtat ttttctaaga tcgttgcagc
    39481 atttcctcgt ccaaaatctt cccaatgtaa taacaattct gggaataatt cagtagctga
    39541 ttgaacgaat tgatcaatga attcgtagta agtttcgcct tctacacgag catgacgatt
    39601 ccctaagtat aatggattat tcaataattc ttcgttatta gtaccagcgt cgattgatac
    39661 aggcaagact tgtgcaggat tgataccagc cgcagcagtg tagaccatca atttaccgat
    39721 agcgatgtca acaccgttca caccccagtc acccatacct aagattcctt ctgcatctgt
    39781 cacaacgatc aaacgaatgt cgcgtccatc tgctgcgttt ttcaaacttg ctttgatatt
    39841 ttctggttcg tcgattgaaa gaaatgcagc atcttgtggt ttcaagaaaa tttcgttgta
    39901 ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt caactaaatg
    39961 ttcgcccatc aatttataaa acaatgtccg gttcgtattg aatagattca ttaagaagat
    40021 acgtttttcc aaatcggtct gtttgcttaa gtattgaccg taagcttgga cagattgttg
    40081 ttccaatgtt tggacagtac ttggaagcat accagtgatt ccgtattttg cccgttcttc
    40141 ttttgtaaaa gctgttcctt tatttagaaa aggattattt aataagttta gccctgtaag
    40201 catgaatgat tcctcctaag ttttaataca aacgaagtat aggagtcaga tgaggatata
    40261 gtcaaaaaga gcaggtataa taaattttat ttatatgagt aatagagtca aaagaaatca
    40321 aaatagcggt atttgttctt tttatatcaa caatccttgc ttgattacta tgcgtatctc
    40381 ttatatgcta aagaaagaaa agtagaagaa taaaggatat gttagataga taaatagaat
    40441 ctctgaagaa taaatcacgt gatcgtgacg ataaaataca cttacgtcag gtgataaaaa
    40501 atacataagt agaaaactaa gaggaggaac agaaaatgaa agaagttcaa ttgatcagaa
    40561 aaagcgaact atcagaaggt ggttgcaatg catgcggcgt agtagaagca acaagctata
    40621 cattaaagct tggtgccaac aaagcgataa tctctgagtt gacagtaggc ggtcttgtgg
    40681 acagtctggc gcttgctgaa ggttttattg gagaagatat ttatgagatg ttttcagaaa
    40741 tccgtcagtt aaaaaaaggg gaaaactgca tagaggttca tcatgagagc ccgaatgtga
    40801 gattcaaacg tggtgataat gagatgatct tcaataatca tgtgtccgat cacaccgagc
    40861 tatatggaat cgtgaatcaa atactaactg agctttttgg attgggacct tatgcgttca
    40921 aggaagaaaa cggtaatcca aaattaaacg aagaatggca agaaacgatt gaaatacaaa
    40981 gaaacaaccc gcacttattt caataggcat ccacagtatt tcgtaaaagg gactttgaca
    41041 cgacttttgt caaagtccca tcgtttttta tctatttgtg ttctaaacat ttctttcaga
    41101 gtagtaacaa ctggaatgac tgaaatgtaa acagattatt tgcctgtatc aatacggaag
    41161 agtaatataa agtgtgtaaa cgctatcata ttattcattc aattttttat agagggaagg
    41221 cattgaaatg gaaaaaacgt atcaagcagg tacaaatgaa ggaatagtgg attttatcaa
    41281 tatggaagat ttagaaatag ccgcttcaca agtgattcca gctggtggtt atggttacat
    41341 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatca
    41401 gctgattatt cctcatgtat taaaagatgt agaattaccc gatacaacta cacattttga
    41461 caaagaaacg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gcttagctca
    41521 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
    41581 aagttcttac gcttcttgta cattagaaga gataagagaa gcaggcgggg agaaagcacc
    41641 gcaatggttt caattttata tgagcaaaga tgatgggatc aatttggata ttcttgaagt
    41701 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
    41761 ccgtgaaaca gaccgcagaa atgggtttac cttcccactg ccaatgccga tcgttcaggc
    41821 ttatcaatca ggagtaggcc aaacgatgga tgctgtgtac aaatcttcca aacagaagct
    41881 tagtccaaaa gatgtagaat ttattgctgc tcattctgat ttgccagtct atgtgaaagg
    41941 tgtccagtca gaagaagatg tctatcgttc attagaatct ggtgctggcg gaatctgggt
    42001 atctaatcat ggtggccgtc aactagatgg tggtccagca gcgtttgatt cgttgcaata
    42061 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgcgg
    42121 acagcatgtt ttcaaagcaa tcgcatcagg tgcagatctt gttgctattg gcagacccgt
    42181 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
    42241 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
    42301 aataaaattg cgtgagaatc gttttattta agaaacaaat gacgaaaaaa ataggagtgc
    42361 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
    42421 aaacgaaaaa ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat
    42481 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
    42541 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
    42601 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
    42661 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
    42721 cctttactga accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag
    42781 tagagccagc agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg
    42841 tagttcccga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
    42901 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
    42961 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
    43021 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
    43081 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
    43141 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
    43201 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
    43261 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
    43321 cggtgaaaat actataggtg aaggattttt taccgcagtt tttgttgaaa ggatgtggtg
    43381 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
    43441 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
    43501 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
    43561 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
    43621 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
    43681 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
    43741 ggaaatcaat gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc
    43801 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
    43861 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
    43921 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
    43981 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
    44041 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
    44101 tacgtctaat gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
    44161 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
    44221 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
    44281 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
    44341 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
    44401 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
    44461 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
    44521 caggtacgat tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg
    44581 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
    44641 tcccggagac gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat
    44701 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
    44761 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
    44821 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
    44881 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
    44941 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
    45001 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
    45061 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
    45121 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
    45181 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca
    45241 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca
    45301 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
    45361 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
    45421 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
    45481 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
    45541 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
    45601 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca
    45661 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
    45721 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
    45781 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
    45841 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
    45901 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
    45961 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
    46021 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
    46081 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
    46141 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
    46201 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
    46261 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
    46321 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
    46381 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
    46441 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
    46501 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
    46561 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
    46621 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
    46681 tctttacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
    46741 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
    46801 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
    46861 cccaaaccgt tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg
    46921 gatactcggc ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt
    46981 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
    47041 aagcaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
    47101 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
    47161 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catggaaact gacagctggg
    47221 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
    47281 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt
    47341 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
    47401 attagaggaa aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg
    47461 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
    47521 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
    47581 cgatgtttat tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct
    47641 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
    47701 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
    47761 aaaaagcggg cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt
    47821 ttggtttgga cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
    47881 ttttttagat ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa
    47941 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
    48001 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
    48061 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
    48121 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
    48181 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
    48241 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
    48301 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
    48361 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
    48421 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
    48481 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat
    48541 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
    48601 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
    48661 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
    48721 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
    48781 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
    48841 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
    48901 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
    48961 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
    49021 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
    49081 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
    49141 gagaccataa cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat
    49201 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
    49261 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg
    49321 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
    49381 aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
    49441 actaagcaaa taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
    49501 aaagtagtat ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct
    49561 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
    49621 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
    49681 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
    49741 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
    49801 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
    49861 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
    49921 ctatttccac aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
    49981 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
    50041 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
    50101 tttggatttc tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa
    50161 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
    50221 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
    50281 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
    50341 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
    50401 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
    50461 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
    50521 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
    50581 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
    50641 aaacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
    50701 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
    50761 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag
    50821 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
    50881 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc
    50941 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
    51001 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
    51061 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
    51121 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
    51181 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
    51241 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
    51301 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
    51361 gcttgttgat gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
    51421 aaatcgatcc gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt
    51481 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
    51541 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
    51601 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
    51661 caatgatcaa gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga
    51721 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
    51781 attattagta gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga
    51841 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
    51901 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
    51961 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
    52021 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
    52081 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
    52141 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
    52201 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa
    52261 aagttgggat cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata
    52321 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
    52381 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
    52441 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    52501 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    52561 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    52621 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    52681 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    52741 tagggagaaa atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc
    52801 tttatatagg tagtttttca taaagacaaa tttgttattc tctcatgatt atcagggtat
    52861 atttttattt attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa
    52921 ttgtttgtag aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa
    52981 ggagatttca ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta
    53041 cggttatatc ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct
    53101 tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt
    53161 gcaatcatta tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt
    53221 ggacaaacgg tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat
    53281 acagaacaag agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac
    53341 tcgctaacta ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa
    53401 acaaaaaatg cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa
    53461 gatggaacca aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg
    53521 attgtattag atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa
    53581 acagaagaaa aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat
    53641 gctggagcaa cggtttattt gacccatact acagatgaat tagtacagct tggagatata
    53701 tgcgactaca gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg
    53761 gaatatgcga atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca
    53821 ttagcacaga caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc
    53881 actgggaact atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt
    53941 tatatgaata atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt
    54001 gctgcaagtt tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa
    54061 caaaaatagc tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt
    54121 gaacaccgtt tattttttta gtagcttcaa agctttttct gtatttgcaa agtgaagttt
    54181 ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc
    54241 agatttcgtt ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa
    54301 ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg
    54361 ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata
    54421 ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg
    54481 ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac
    54541 ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata
    54601 ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt
    54661 tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc
    54721 acatactgtt agcggaccga aatagctgcc atttccgacc tcatcactac ctaaaacgga
    54781 taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc
    54841 ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt
    54901 tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg
    54961 aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt
    55021 tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata
    55081 ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt
    55141 tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt
    55201 catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa
    55261 aaaacacgct acaaagcggt gattgccaac caaacatata ctattatcgg acgggaaacc
    55321 aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa
    55381 ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg
    55441 gatcaattga agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag
    55501 aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag
    55561 aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat
    55621 gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct
    55681 aattaagatc cttttatcaa aataagaaaa atttaaaaaa agttagaaga gaaaggaacg
    55741 tagcatgctg agtttgctga ttttattttt attgctaatt gcgttttttt ctggagcacg
    55801 tcgcgggttt gctttgcaaa tcatattcgc gattggatat gtattgtcat ttattgctgc
    55861 ccagcatttt tacaaaccat tagcaagtca tttggaatta tacattccat acccagctgt
    55921 cacgccaaca tcaaagttag cattttttga tcaggtattt gcctttcacc tagatgaagc
    55981 tttttatgca ggaactgctt ttctgattat tttgctaatt ggctggctgc ttacccgatt
    56041 tgtgggggtt tttgtccatg gattgacgta tgtgccgatc ttgagacagg tagactggat
    56101 tgcaggaggc atcctgtcac taatcatggc ttacgttact atatttttga ttttgcagtt
    56161 acttgccttt gttccattag atgtggtcca aaatcaattc ggcgagcata gtttcgctcg
    56221 ctttatcgtt gagagaacgc catttcttac caacaagatg tatgagcttt gggtaacgaa
    56281 tattttgaat taaggaaaat agtgctggaa aatatttcgt tttttgtaca gagtagatta
    56341 tttaagaagg ctgcgtagcg tatgtccggc acagctcttt tttcatagtg aaagataagg
    56401 tgataaaatg aataaccgaa tccttgaaac attggaattt gaaaaagtca aacagatggt
    56461 cagacaattt gtagtgactg cacaaggaaa agaagaactg gcagaattgg taccagtgag
    56521 cgaggaacaa acgatcacaa actggcttca agaaactgag gatggattga aagtgcaacg
    56581 tctgcgaggc gggatcccta tacctaaact tgaaaatatc cgtccccata tgaaacggat
    56641 tgagatcgga gcagacttga atggagtgga gcttgcacaa gtttccagag tattgtctac
    56701 gacaagcgaa ttgaatcgtt tcattgatga tctttatgac agtgaaatcg aatttgcacg
    56761 tttgtatatg tgggcagatc agttagtcac gatccctgtt ttgagccgcc gtttaaagga
    56821 agcaatcgat gaagacggac gcgtgactga tgacgcttct cctgagttaa agagcataag
    56881 acagaacatt cggcgaagtg aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg
    56941 gaaaaatgca aagtatttaa gtgatgcgat catcaccatg cgaaatgacc gctacgttat
    57001 cccagtcaaa caagaatacc gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc
    57061 aggacagact ttatttattg aaccaaaaca agtggtggat ttgaataatc gtttgcgtca
    57121 ataccaaatc gctgaacgaa atgagattca acgtatttta agtgaacttt ctgctgaact
    57181 tgttccccat cgtcaagaaa tcatccacaa tgcttatgtg attggtaaaa tggatctgat
    57241 gaatgcaaaa gcacgatttg gtaaagaagt aaaagctatc gtgccgggaa tcagtgaaga
    57301 caatcatgtc gtcttgaaac aagcaagaca tccactgatc gatcaggaaa aagtagtttc
    57361 taatgacatc acaatcggaa aagactatca agccatcgtc attacaggac ctaacacagg
    57421 tgggaaaacg atcacgttga agactttagg tctgttacaa ctgatggggc aagcaggatt
    57481 accgattcct gcaggtgaag aaagtcaaat cggtattttt gaggaagtat ttgcggatat
    57541 aggagacgaa cagtcgattg agcaaagtct atcgaccttt tcttctcata tgacgaacac
    57601 tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta ttatttgacg agctcggtgc
    57661 aggaacagat ccgcaagaag gtgctgctct agcaatcgca atcttagatg atcttggtaa
    57721 aaagtctgct tatgtgatgg caacaacgca ttatcctgaa ttgaaagttt atggatataa
    57781 tcgggcaaat acgattaatg ccagcatgga gtttgatgtg gatacattaa gtccaacgta
    57841 tcgtttgctg attggcgttc ctggacgaag caatgcgttt gaaatctcta gccgattagg
    57901 tcttgataca gaagttattg atgaagcgaa gcagttgatg aatgatgaga gccaagattt
    57961 aaacgaaatg atcacagatt tagagaatcg ccggaaaatg gcagagaccg aatatctgga
    58021 aatgcgtcat tttgtttctg aggcacaaga acttcacgat gatttgaaag aagcttacag
    58081 ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt
    58141 cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct gatattcgta aaatgcaaca
    58201 gcagattggg caaggaaatg tgaaagagca tcagttgatc gatgcaaaga cccaattagc
    58261 aaatctacat caagaagaaa ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca
    58321 aaaaacgttg aaaccgggag acgaagtgtt ggttacgact tacggccaaa gagggacgct
    58381 tttacgtaaa aacgggaatc agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc
    58441 cgaagacgag ctgacgcctg ttgctcctca aaaagagccg acacaacgag ttatccatgc
    58501 tgtacgatct gaatctagta gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata
    58561 cgaagaagcg ctaagtgagg tagatcaata tttggattct gccattctag ctggttatcc
    58621 tcaagtgact attgtccacg gaaaaggaac aggcgcactt cgcaagggaa ttactgatta
    58681 tcttaaaaac catcggagcg tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa
    58741 tggtgcgaca attgttaaat ttaagtaaag cttactaaag gtaagcagat ggttcgctat
    58801 ccaagtatag aaatgctata ctagtcatag attttaaaaa tggaggtctt tgatatgtca
    58861 caagtaatta cagataagac atttaatgaa gaaacagata aaggacttgt attgattgat
    58921 ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac cgattttgga tcagttggaa
    58981 caagaatatg atgaagaaga attcagaatt gctaaaatgg atgtggatga aaatccagaa
    59041 actcctcaac aattcggtat catgagcatt ccaacattaa tgttgaaaaa agatggtcaa
    59101 gtagtagaaa aagctgttgg tgttcattca aaagaacaac tacgtcaaat gatcgatcaa
    59161 tatttataaa aacttacagc aaaaataagc tgagggtggg actgaatcca gttccgtcct
    59221 cggttttttt gcggttgagt taaggttatt caaattaata ttttttgagt ataattacgt
    59281 tgtcaggctg gattgttttt ttgacagaca tgctataata actttaattg aatatgaagg
    59341 ggagtaccag atgaacgaac gaatcaaaaa caaattagct ttacttcctg atcagcccgg
    59401 ctgttacttg atgaaagata aaaatggaac gatcatttat gtcggcaaag ccaaaatatt
    59461 aaaaaatcgt gtccgctcat attttactgg tagccataat acgaaaacgg aacggcttgt
    59521 tagtgaaatc gaagactttg aatacatcgt tacagaatcg aatatcgaag cattgctctt
    59581 agagatcaat ctgataaaga aaaatgatcc taaatacaac attatgttaa aggatgataa
    59641 aacctatcct ttccttaaga taacgaatga aaaatatccc cgtttggtga ttactcgtaa
    59701 ggtactaaaa gacaaagcac tgtattttgg accatatccc gatgtcggtg cggcgaatga
    59761 gacaaagaag atcctcgatc gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa
    59821 ggagccttgt ctttactatc atttgggaca atgtctgtgt ccttattatt tcgacgtaga
    59881 tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaac tttttaaacg gaggacacga
    59941 aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct gctgaaaaca tggagtttga
    60001 aaaagctgca gaataccgag accagatccg tgccattgag acaatcatga ctcgccaaaa
    60061 aatgacaaat actgatttac tggatcgaga tgtatttggt tatgcgattg ataaaggatg
    60121 gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta attgaacgag atgtttcaat
    60181 gtttcctttc tataacgaag cagaagaaga tttcttgact tacctcggtc agttttatca
    60241 ggaaaatgag catttcattc ctaaagaagt cttgattcct gatacgatcg acaagcccag
    60301 tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt
    60361 agttgaattg gcgagtaaga atgcctcagt tgctttgaat gaacggtttg atctgattgc
    60421 cagaaaacag gaacggacga tcggagcagt tgaacgttta ggaaacgcga tgaatatccc
    60481 gaccccaata cgtatcgagt cttttgataa ctctaatatc atggggacga atcctgtggc
    60541 tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa gaatatcgga aatataaaat
    60601 aaaaacagtc aacggaccgg atgattatgc atcaatgaga gaagtcatct atcgcagata
    60661 ttcgcgagtg attcgcgaag ggttgccttt acctgatttg atcttgatcg atggaggaaa
    60721 aggacaagta gatgtggcaa aagatgtctt agaaaatcaa ttaggaatcg atattcctat
    60781 tgctggcatg gcaaaaaacg ataaacataa aacaaatgag ttgctattcg gtacagattt
    60841 ggctgtagta ccgcttgagc gaaattcacc agagtttttc ttactccagc gaatccaaga
    60901 tgaagtccat cgattcgcaa tcactttcca tcggcagcta cgcagtaaaa acagttttgc
    60961 ttcccgatta gatgaaattg aagggttagg accaaaacga aaaaaaatgc tgcttaaaga
    61021 gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa gaattacaag aaatcggact
    61081 gccgaaaaat gtcgcacaaa acgtatttga taaattacat caggaatgag gaaagggcac
    61141 tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa caaaaagagc gtttacgttt
    61201 tgttggtaaa taataaatcg cttacgtctg gtgtctaagg atatatgtga tttcttgaag
    61261 aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa acttttggga actacacagc
    61321 tgtcgatcat gtctctttcc aaatccccga tggaaaaatc atggggctaa ttggccaaaa
    61381 cggggctggg aagacaacga cttttcgttt gatcttggat tttttgacac aagaccaagg
    61441 agaagtgtta tggaatggcc atcatcttgg ggagaaagat tatgatgtca tcggttatct
    61501 tccggaggaa cggggacttt atccgaaagt aacgattcaa gaccaactgc tttattttgc
    61561 agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa
    61621 atttcaggta aaagggaaaa agacggataa agtaaaatct ttgtctaaag gaaatcagca
    61681 aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa ttgatcattt tggatgagcc
    61741 attcagcggg ttagatccgg taaatgctga attgctaaaa gatggaatat tggaattgaa
    61801 aaaacatggt tcatgtgtca ttttttctag ccacaatatg gataatgtag aaaaaatctg
    61861 cgatcatttg atcatgttgc gaaacggaaa aatggttttg aatggaaaag tacatgagat
    61921 tcgtgaatca tttggccgta cgaagttatt cttggaatct ggtttaagcc aacaggaagt
    61981 agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa gacaatacgt tagagattac
    62041 cttagaagat cctgaagctg ggaaagaaat ctttactcgt gcgactcagt tcggctacat
    62101 tcctatgttt aaccagcaac cgccaacttt ggaagagatt ttcaaattga aagcaggtga
    62161 aatcgatgcg taagttttgg attattacca aagatgtcta tttaaaaaat gttaagtcga
    62221 tttctttctt gatcatgata ttagtccctt ttatcatgct gggtatcatt tatgtagcag
    62281 gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg cgtattggct gataatgagc
    62341 aaatcagtca gcaaatgagc caagtcaaaa cagatgattt tgagttcaaa gcagttgatt
    62401 ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct
    62461 caactgagaa tgataagata aatgggatgc tttatagcga aaattcatta ggacaaagta
    62521 cacaactact gatacaacaa caactgactg gcttgcaatc gatgcttcga gcgagtcaat
    62581 taggcatttc accagaagaa gtagctagct tgagccaaca agcaagtttg actcgacaaa
    62641 aagtcaattt tgattcaaaa ggaaaaatga cagtcggaga agataactcg acagtccaat
    62701 acgtcgtaag ttatgtagca acgattgtct tgttcattat tattttgacg tatgcgcaga
    62761 tcattgcaca agagatcgct tctgaaaaag gcacgcggat catggaagtg atcctgtcca
    62821 gtacgactgc acaaaagcat ttttacggaa aattaacagg tgttttactt gttgcattaa
    62881 cgcaaatggc attatacggt attatttttg gtataggata cagtcaattc aaagatatgg
    62941 aaatggtcaa aaatatatta gacggtatct cattggataa cttgattgga ccattcttgt
    63001 ggtattctct tctattcatg atctttggca ttttgatttt ttctgtttta gcagctttat
    63061 gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc aattttgcca gtgacttatc
    63121 tatctctagg cggatacatg cttggattga tactaggagc ttcagatcca aacaatatcg
    63181 ttattcggat cacttcgtat attccattct tatcttcata tattatgccg gttcgcttag
    63241 caaatgagac agtcggtatg ggtggagcat tgatttcctt gttggtactt gtagccgtaa
    63301 cattcttatt gatgatcttg tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg
    63361 aaggcggatt atggagttca ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa
    63421 aagcataaaa caatcctaaa atacagaagg aagctggaca tctattgttc ccagcttctt
    63481 tttgtataga cctattttat ttattttact atattggata tatattcatt gaaaatagtg
    63541 tataacaact ttataatatt gagtatacca atttttggag gcgttttaat ggaccgttat
    63601 ttagatttac tttcagaaaa attcccctca acggaggatg tcatcacgga aatcatcaat
    63661 ctcgaagcaa ttttgcagct tccaaaagga acagagttat ttttgagtga tatacatggc
    63721 gaatttcctg cttttgatca tattctgcgt atcggttcag gaaatcttaa agaaaagatc
    63781 aaagaccttt tttctgaaca aatgacggaa acggatatca gccaattcac tatttttacg
    63841 gcttatccag aatatgcatt aacgactgat tggtacaaag aacaagataa atcgcagctg
    63901 atccatcatt tgatcgatct tttacggttt acgacagtca aatatacccg gtcaaaagtt
    63961 cgaaaaggat tgccgaaaga atatagctat atcattgagg aactcttgta tatcgatgac
    64021 cgtatcaatg gaaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag
    64081 gaagaagaaa aatttttaac gaaacttgca cagacgatcc aaaaatctgt catcgatcat
    64141 ttgcatatcg ttggagatat ttttgaccga ggaactcaag cagccaaagt gatggatcga
    64201 atcatggatt tttcttctgt agatatccaa tggggaaatc atgatatttt gtggattggt
    64261 gcgtattgcg gctctgaggc atgtttactg acacttttac ggattgcagc acgctataat
    64321 tatttatttg aattagagaa agaatatggg ttgaatttac gtccattgtt ttcttttgct
    64381 catcaaactt atcagaagaa tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa
    64441 agagagcaag aagaattaga aaaaatccat caggcacttt ctatccttca attcaaatca
    64501 gaacatcagt tattggatcg tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa
    64561 aaaatcgact atgaagcatc aacgattgat ttagaaggtc agctctatga gttgaaagga
    64621 acttgttttc aaacgatcca gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa
    64681 gtgattgata gtttgatgta ttcattccag cactctttga gaatgcaaaa acatatgacg
    64741 tttttgatcg aaaaaggggg aatgtatctc acgaacaatc agcatgtgct gtttcatggc
    64801 tgtatacctg tagacgagaa gggacagctc ttagcatttg aattagacca atcttatcgc
    64861 ggaaaagaac tgctgggctt ttttgaaaaa cagataactg aaagcgcaaa agatctgact
    64921 gcaaaagaag atttagcaac cgatttgatt tggtatgcgt ggagtgggcc attttcgcct
    64981 ttattcggca agagtaaaat ggcgactttt gaacgttatt ttctcacaga gcctcatacg
    65041 cacgtcgaga aaagcaatcc ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa
    65101 attttagcag aattcgacac aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca
    65161 gtaaaagtaa aaaaagggga gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac
    65221 ggaggtttat ccaaagctta tcaaaaaacg acaggaattg ccggttattc actattatgc
    65281 aattcttatg gtttccaaat cgtgacacat tatccgtttt tgcctattga acaacaattt
    65341 gcaaaaaaat ccgaccaaac aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa
    65401 ctcaatcgtg atacaacaaa aggtatggaa ctgcagcagc agattaccca gctcaaaagg
    65461 ctgttggact atcgaaagga cgattgagaa gaattccagt gaacattgtt ccatagactg
    65521 gagactgaga caaaagtcgt gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc
    65581 aactcctcgg tcacaagtca cactattcaa caaacatgaa aagctgtttt ttgaaattgc
    65641 tccttgtgac tcagagttga acggcaggct cacgacctct tggtataaac ggtgttcaaa
    65701 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa
    65761 atttttctta atactcgaaa ctaaacagct ttttcacaac cccctcagaa agtctattaa
    65821 aattcattca caaagaaatc gaataagcgc tgttcagatg gatgtacacg gtgggtaagt
    65881 tccggatgcc attgaacacc gaggatttta tgcatatcat ctttgctttg aaccgcttca
    65941 atgagtccat cgttagaaaa agcgatggct tttaataaag gtgaaagatc tttgactgcc
    66001 tgatgatggt aagaattcac aaaatatgga tcggggagaa ggttgcttaa caaactgtct
    66061 gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt
    66121 actgaccact ttggatataa tgaaagatct tgataaagtg taccttccaa aacgacattc
    66181 aatagttgga gtccccggca gactgcaaaa attggttttc tttgtttcaa tgcttccaag
    66241 atcagtgcct gttcaaattg gtctcgttga atatttgttt ccgttagctt tggatgagga
    66301 tcttccatgt aaagttgtgg actgacatct tgaccacctg caagaataag tttgtcgatt
    66361 tgtccaatca gttgaggagc tgaatctgga gacatgactg gcagaataag aggcaatcct
    66421 tgagcatcta aaactgcatc cacgaatcct tgcggtgtat agctcacttg atttccttgg
    66481 aaagtatctg ttgctcgaat cagttgattt ccggcaatgc cgatgattgg tttcatttgt
    66541 attcccccta aagtagactt attgtaacaa ggatttaaaa aaaattaaac tatgtaagac
    66601 ttgcccctat acttatctgt tcttgctgtg ttactatctg taaagagaaa tttcgtgtga
    66661 aattcatgac gatagttgaa aatattgctt aatttgttat aataaaaagt aagcagaaat
    66721 aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa ggacatttta
    66781 ttgaaggggc ctgcctatac taagttatct ttagaggtac atactttcaa tgaaaaaaga
    66841 aagcatgtac ctctttttta aatcgaaagg aagtgaagca agtgttagaa gctttggaac
    66901 aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca aacactgcgg cagcagctga
    66961 agatctatgc aacagaaaaa gagcaggaat tggaaacgat ccaaacaaac tatcgacaac
    67021 aactaaaaca gttggtggaa gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa
    67081 aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac tttgaaagat caatatcaaa
    67141 agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc
    67201 actaagatgg aaaaagtgac cctcatttca gataaaagaa atcaggagag gattttacaa
    67261 gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt atcaaaatag aacaaacaac
    67321 cagtgggtgg agaagtattt tccagatacc acgattattg ataaagaagc aaaattaagc
    67381 gctttaggta atcggttgaa agacatccaa gaagctatcc aattcataca acaccacggc
    67441 gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac tttccttaca agatttggaa
    67501 gccaattatt ccgaagaaaa tttcataaat aaattgaaag aagtccttga tttaaaagaa
    67561 aagtgggaac aactaactga agaacgtgat cgtttagaag aagaggaaga atggcttctt
    67621 cattggcaac agctggatat tgctccaagt atgtatgcaa gtaagacgac catttttgag
    67681 atggggactg tgagtgcagc aaattttgaa agtttcaagg aagaactgag taagcttgat
    67741 gacgtttata tggaagaaat agattatacg ccaaaacaag tttatctttc gtatgttgct
    67801 ttaaaaaaat cagttgctgt tatagaagaa attgctggga aatacgggtt caataaagaa
    67861 acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag agaccaagca gcaattatcc
    67921 attgttcacg agcagcaaaa gaagataacg acagcgttag gtcagtgcag tggctatatc
    67981 caagaatttg aatgggtaga agaagtcaca cttgccgttg cagaacgaga aaaaataaaa
    68041 gaacggttta tccatgcgac ttatctgatc gttttgcaag gttgggtaga tgcagaagaa
    68101 aaacagaatt tgctgcatgt gttaaacgaa accgtatcag aagaagatat atacgtcagt
    68161 ttcgaaacac caaactcaga agagattcag caggaagtcc ctactaaatt gaaaaaccat
    68221 ccgcttgtcg aaccttttga attgctgact gaaatgtaca gtctgccaaa atatgaagaa
    68281 gtagatccaa caccttggat gacaccattt tatcttgtct tcttcgggat gatggtcgca
    68341 gatgttggtt atggtttgct gatgctcatt gggacaatac tagctcaaaa actacttgtt
    68401 ctaccacgcg ggatgaaacg tttcgtaaaa ttctttgaga ttctctcgat accatcgatc
    68461 atctggggat tgatctacag ttcatttttc ggacagtcat taccaaaaga actgtttggc
    68521 atccgcttgc ctttcccaat cctatctacg acggatgacg tcaatacgat tttgatttta
    68581 tccgtgattt tcggtctgat acagattttg gttggattat ttgtggcagc aaaggaaaat
    68641 atcaggagaa aagattactt gagtgctgtc agtgatggat ttgcctggca agggatccta
    68701 attgggatcg ttattgcgtt ggttggtgcc atgttgataa aaaatacagc atttgtttac
    68761 cttggcgctg ggctatcgat cattgctgcc ttatgtatct tgatcgttcc gattatccaa
    68821 acgccgtcta aagtcaaagg tgctgccaaa ggtgcgtata atctttacgg actgaccggc
    68881 tatatcggtg atttagtcag ttatacacgt ttgatggcat tgggtatttc aggcggaagt
    68941 attggagcgg cattcaatat gctagttgct ttcatgccgc cagctgctcg attcagtgta
    69001 gggatccttt tgatcatcgc tttacacgca ctgaatatgt ttttgacact gctaagtgcc
    69061 tatgtccatg gcgcacgatt gcagtatgtc gaattttttg ggaagtttta tacaggaggc
    69121 ggacgtgcct ttgatccgct gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa
    69181 aaaaacaata aataattgga ggatttttat ataatgatgg attacttaat tagtcaaaat
    69241 ggtggaatgg tatttgcggt attagcgatg gcaacagcga cgattttttc agggatcggt
    69301 tcagctaaag gtgttggaat gacaggtgag gcagcagcag cattgacaac aagtcaaccc
    69361 gaaaaattcg ggcaggctct gattctgcaa ttgcttccag gtacgcaagg tttgtatgga
    69421 ttcgttattg cctttttgat ttttatcaac ttaggaaatg acatgtcagt tgtacaagga
    69481 ctgaactttc taggtgcttc tctgccgatt gcttttacag gcttgttttc aggtattgcc
    69541 caaggaaaag tcgcagcggc aggtattcaa attttagcga aaaaacctga acatgctaca
    69601 aaaggaatca tttttgctgc gatggttgaa acatacgcga ttttaggttt cgttatttcc
    69661 ttcctattag tgttgaatgc ctaagcataa aggaggaaaa gcagatgaac gccattgaaa
    69721 aaatcatctc tcagatgaac gaagcagcag agcaggaaag agcggcgtta gaacaagaag
    69781 agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac gcaagttgaa acagaacatc
    69841 aaaaacaaaa agaaaaacaa atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc
    69901 gccagcaagt agaagtacga caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat
    69961 tgtttgcaga tgcggtaact gaaatggaaa attgggatga atctgaacaa atccagttta
    70021 tcaaaaatgc attgtattca ttacctttga caggaaaagt agcattcatt gctggagaaa
    70081 aatcagcagc ctatctttct cagacgttgt tggatgaatg gaacaatgaa cttcccttta
    70141 tgatggtttt aagtgatgaa actgttgctg atcaagcggg ttttctgata aacgatcaag
    70201 gagttcagta taattttctt ttcagcagcc ttgtacaaga tatccaagga acgatgagtt
    70261 ttgagattgc caatcaatta ttcgaataag gagggatcag tgtatggaat accacgaact
    70321 aaatcccttg attcgcggaa gagagcttga acttttgtcc aatgagacat ttgaacggat
    70381 gattcaaaca aattctgtcg aagcacttgg tgaaatattg aaatccacga tttacagtcc
    70441 atatatttat gacggatatg aatatgactt cgaacagaat cttttaaagg agcagagccg
    70501 tctatttcgc tggttaaagg aatcagcacc ggagcctgaa atcgtttgga tctatacgat
    70561 gcgttatact ttccataatc tgaaggtttt gacaaaagct gagatgacaa atcaaaatct
    70621 ggaccatttg tatatcgacg atggatttta ttcattagaa accttgaagg atgccattca
    70681 tacgcaagtt tcaaatgaac ttcctgaaag cattatggac agtatacgag aagtacgtga
    70741 ttattttgaa gagtctacca ttcttcaggg aattgacgtg atttatgatc gacagttttt
    70801 gacagagcag cgacggcttg ccgataagct aggatatcaa gaattgctga cagaagtcat
    70861 tgcttttatt gacttgacca atatcacaac gatggctcga ggaatattgc agaaaagaac
    70921 atatgggttc atgacgacgg tactttccag ctcgggaagt atcgaaaaag aagtattttt
    70981 gaactttgta gataaagata tggaagatta cacgaatttt ctattagaga cagattatgc
    71041 tgattttctg cgccctgtca tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt
    71101 gaaagatgat tatctaagtt cattgtatca agaagcacaa acccaggctt tcggaccact
    71161 gccactacta gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt
    71221 tatcggcaaa agaagccatt tcgaatcaga aacaatcaga aagcgggtga gacaagtcta
    71281 tgacgcataa gatcggtgta gttggcgaca aagattctgt tttgccattc aaattgtttg
    71341 gctttgatgt tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg
    71401 caaagaatga gtatggcgtg atttatgtca cagagcaatg tgcagcattg agcccagaaa
    71461 cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt attgattccc aatcatcaag
    71521 gatcattagg gatagggctg gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa
    71581 atatcttata gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct
    71641 ccggacctct tgtaatggct gagaacatgt ctgatgccag tatccaagac atgtgtctag
    71701 tgggagatct aggagttatc ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc
    71761 aagtatatga agagacttct ggaattggtc caggcgaacc ggttcgctct actggtgaag
    71821 ctttatctgt tgaattagga ccagggatca tttcacaaat gtttgacgga atccaacggc
    71881 cattagatac ctttatggaa atcacacaaa gtaatttctt agggcgcgga gttcaattgc
    71941 ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg
    72001 tgagtgctgg agatgttatc gggatcgtag aagaaacaaa agttataaaa cataaaatca
    72061 tggtgccaaa tgggataaaa ggaacgatca aaacaatcga aaaaggttca ttcactattg
    72121 atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa
    72181 aatggccagt aagacgtagt cggccaatca aagaaaaatt aaacccggat gcacccatga
    72241 tcactggaca gcgagtgatt gatacattct tcccagtgac aaaaggagga gcagcagctg
    72301 tacctggtcc ttttggtgca gggaaaacag tcgttcagca tcaaattgca aaatgggccg
    72361 atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt
    72421 taaatgaatt ccctgaattg attgacccta atacaggaga atcgttgatg gaacgtacgg
    72481 tattgattgc taatacatca aacatgccag tagcagctcg agaagcttct atctatacgg
    72541 gaatcacaat tgcggaatat ttccgtgaca tgggttatga cgttgctatc atggcagact
    72601 ccacttcacg atgggcagaa gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg
    72661 gtgatgaagg ttatccagct tatcttggtt cacggcttgc ggaatactat gaacgatctg
    72721 gaagagtcat tgcattagga tcagaaggtc gcgaaggaag tataaccgca atcagtgctg
    72781 tatccccgtc tggaggggat atttcggaac cagtcacaca aaatacattg cgggtcgtaa
    72841 aagtattctg gggtcttgat tccagtttag cccaaaaaag acattttcct tcgatcgatt
    72901 ggatacaaag ctattctctt tattcaacag aagtagggaa gtatatggat catatccttc
    72961 aagctgactg ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc
    73021 tgaatgaaat cgtgcgtttg gtagggattg attccctttc agataatgac cgtctgacat
    73081 tagaagtggc aaaatccatt cgtgaagatt atttgcagca aaatgctttt gatgatgtcg
    73141 atacattcac ttcaagagag aaacaattca aaatgttgaa gatgatcttg acctttggtg
    73201 aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg gacggtaccg
    73261 tatcgattcg tgaaaggatc ggacgaagca aatatatccc ggaaacagaa ttagaaaaac
    73321 tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca
    73381 tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt
    73441 gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca aaatggcgag
    73501 attcgtcgtg gacaagtatt ggaagtccaa gaagataaag caatggtcca aatattcgaa
    73561 ggaacaagtg gtatcaattt acgtgactct tctgtacgat ttttaggcca tcctttagaa
    73621 ctaggtgtat cggaagacat gatcggtcga gtattcgatg gtttgggacg accgaaagat
    73681 aacggaccag agatcctgcc tgaaaagcat ttggatatta acggagaagt aatcaatcca
    73741 gtagctcggg attatccaga tgagtttatc caaacaggta tctctgcaat cgatcatttg
    73801 aatacattag ttcgtgggca aaaattacca gtattttctg gctctggtct tcctcataaa
    73861 gaattagctg ctcagatcgc gcgacaagca acggtactcg attcatcaga tgatttcgcc
    73921 gtagtctttg ctgctattgg aatcactttt gaagaagctg aatttttcat ggaagatttt
    73981 cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga atttagcaaa cgaccctgca
    74041 atcgaacgta tcgctactcc tcgtatggct ttgactgctg cagaatatct tgcttatgaa
    74101 aagggaatgc atgttttggt tatcatgaca gacatgacca actatgcaga ggcattgcga
    74161 gagatttctg cagcacggcg agaagttcca ggaagaagag gatatcctgg gtatctttac
    74221 acgaacttag ctactttatt cgaacgagca ggaagaatcc gcggactaaa aggatcggtt
    74281 actcaaattc cgattctaac aatgccagaa gacgataaaa ctcatccaat ccctgacttg
    74341 actggttata ttacagaagg acagatcatc ttgactagag agctttacaa gagtgggatt
    74401 cagccaccaa ttgatgtact cccgtctctt tcgcggttaa aagataaggg aactggaccc
    74461 ggaaagacaa gagaagacca tgcagctacg atgaatcagc tttttgcagc ctatgcacaa
    74521 ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat ctgctttatc tgatgtagac
    74581 aagatctatg cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca aggattctac
    74641 acgaaccgtt cgattactga aacacttgat ctagggtggg aactgctatc gatgttgcca
    74701 agaaccgaat taaaacgaat caaagacgac atgctggaca aatatctgcc agaagggaag
    74761 tgagttctga tggcacgatt gaatgtgaat ccgacccgca tggaattgac tcgattgaaa
    74821 aaacagttga caactgccac aagagggcat aaattattaa aagataagca agatgaactg
    74881 atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga aatggaagag
    74941 tctatgacag aagcaatgtc tgattttgta ttggcaaatg cttcgatcac ggaacctttc
    75001 atcgaagaat tatttgtttt gccggcggaa aacgttgatt tgtctatcgt agacaaaaat
    75061 atcatgagtg taaaagtacc tgtgatgaat tttgaatatg atgaacagct gacagacaca
    75121 cctttggaat acgggtattt gaattccagt gccgaactgg atcggtcaat cgatcggttt
    75181 actaccctgc ttccaaaact tttggaactg acagaaatcg aaaaaacttg tcaactgatg
    75241 gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat tagaatatat gacgatccct
    75301 caattagaag aaacgatcta ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa
    75361 gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg actaagtggt atcttttttt
    75421 aaaagaggaa aacgatgatt aaacaaagca aacaaataaa gaaacgtttg ccggctgttc
    75481 agcttctcgc attaggattt tttctcctga ttttagttgg cggcggccta cttaccctac
    75541 cattttttag taatagtggg catgggacaa attttatcga tgcgcttttc actgcaactt
    75601 ctgctgtctg tgtaacggga ttgacaacat tgaatacagc agagcattgg aacagtgctg
    75661 gtcaattttt gatcatgctt ttgattgaga tcggcggtct tggttttatg atggttccga
    75721 ttctatgctt tgcattgata aagaagaaag tcagtctcag cacacgtatt atattaaaag
    75781 aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct catgctttac atcctaaaat
    75841 tggcctttgt gatccaattg attggtacgg ttgctttatc ttttgtattt gtgcctgaat
    75901 ttggctggtt gaaaggtatc tggtacagtt tgttccatgc aatctctagt ttttgtaatg
    75961 cagggtttga tttgttagga gacagtttag ccaatcacca gcaaaatgtt tatttgatca
    76021 tggtggtatc tgctttgatc atttctggtg ggttaggctt catcgtatgg cgtgatttac
    76081 tggaatatca tcagaaaaag aagatgacga ttcattcaaa aatcgcatta accgttacag
    76141 gtaccctttt attaggcgga ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc
    76201 tagtggaagg gaatatgttt caacgattgg caaacacctt ttttatgagt gtgacaccta
    76261 gaacggctgg atattattca gttgattacc tccatatgag ccatgcaggt ctgattttaa
    76321 cgatgttttt gatgtacatc ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa
    76381 ctacgttagg tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt acgagagcgg
    76441 aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact ttattttttg
    76501 ttacgttgac gttgtgtgta gtcgccatca tgattttatc ggtgactgaa acgataccta
    76561 aaacttctgg catcgaatat attgcttttg aggtcttctc tgcgtttggt acagtaggtc
    76621 ttacgatggg attgacgcca gacttgactg caatagggaa attgatcatt atagcgctta
    76681 tgtacattgg acgagttggt gtattgactg tggtcttctc tctgatgaca aaagcacaaa
    76741 gaacacaatc aaaatacaaa tatccagaag aaagtgtcat gattggataa tttcatcttc
    76801 tgacagaaac ctagtgattt agtaaaataa gaaaataaaa taatttttct tgcttcttgg
    76861 atactttaag tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt tgaacctcgg
    76921 gtagtgcctc ttttttattg aaaggaatca ataagatttg attcctttat ttatcaatag
    76981 gttataattc atattttttt attttttctt ttacgatact tagtccagaa gctaaaattt
    77041 caaggttatt agaggatagg gaaattcgaa cgaatgcatc tggttgcgta cgtgtagtaa
    77101 agcgtctgga atgataaagt cggataccgt tagcaagaaa gtcattctca attatcttag
    77161 ggtcaatctc aggatcaact ggaagaattc gatagtaagg gacacgattc gttggttttt
    77221 ctactttgaa aagagaatca aaaagctgat tggctctttt tgtaagactt aatttagttt
    77281 ctaagatcct atttgcttca tttgaatgga ggatttgagt gatgacttcc acatctaatc
    77341 cagaagtttt cacatttata ttaaaaattg cttgttctat cttgctttgg acatgtactg
    77401 gaactacaag ataagcaact cgcaatccag aacaaataaa ttttgtcatt ccagctaagt
    77461 agattgtttg gttaggtaat aaattttgga aaggaggaat gatcgtatgt tgatagtaag
    77521 ttgttaaaaa tgaatggata tcatcttcaa taacccatag accttctttt tcaatcactt
    77581 cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc tataggatta ttacaagaag
    77641 gcataagaaa gacaccatga atcttctttt ttctacattc ctgaatcaat atatcaggtc
    77701 gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa atgatataac tgagccaatt
    77761 caatgaaatt agcgtatgta tatcggtctg ttgctattct atcacctgga gagaaaagtg
    77821 cagaaagtgt gatagccaat ccattttgaa caccggaaac aatagataga gattctaagg
    77881 acggaatgac tccttgagtt tttaaccaat ttgctgcagt agtcaattga aaatttgtgc
    77941 ctttgggatc ttggtaatta agcaaaccaa caagatttgg gttattacta gttgattgaa
    78001 taaacgggac aacgaattta ttacattctt caaaagaact aaccagacca aaatcaatga
    78061 tatgttcggt tacttcattg gtagagatgg tgatggactc aaagacattg ggagaaataa
    78121 atgtgccact tccaatattc gtatacagca aacctttctc aattccatat ttataagcct
    78181 tgccaacggt agtaaaattt aaatctaaat aatcagctag ctctctttgg gagggtaact
    78241 gagtgttttt ctgtaattta ttctttaaaa tatcttgttc taattgttga atcaacgatc
    78301 gataataagg acgcttcaat ttttgtttgt ctggtcgcca agttaacggg taattgtcat
    78361 aagaattaat aggcattttc tccactcctt aaaattgtat accatacaat tttaccattg
    78421 attgtattga ttttagaagt taatcttttg tgaggaggga attgtctatg aaaattatta
    78481 attttgagga agtagtagct tcttttagtt ttgaagaagc gattcaagta atgaaaaaat
    78541 gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg gaaattttac
    78601 ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg gagaacagat
    78661 attttggcac aaaagtcatt accgcttttc cggataatca tcagtatcat ttaccttctc
    78721 atttaggaga gattttcttg tttgatgctc aaaacggact acccaaagca ttgataaacg
    78781 caaatgcagt cacctggatt cgaacagctg ctgtatctgc gctggcaaca gattacttag
    78841 caaatccgca tgcagaaaca cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt
    78901 tgactgcgat ttctaccatt cgtcccatca ctacagtgtt tgtttacgat attttagaag
    78961 aaagacgaaa tacttttatc gctaaagcta aaaaacagta tcctaaaata acgtttataa
    79021 atgcaatatc agtagaagat gcagtacgtc cagcggaaat catttgtacg ttgacctcta
    79081 gtaaagaggc atttcttcaa aaagagtggg taagggaaga cgcacatatc aatgctgtgg
    79141 gcacatttac tcctgataca agagagataa gtacatcgtt gatggaaact tctcaaatct
    79201 atgtagatga ttactcagct gccttaacag aaagcgggga cctgcttata gccatggcag
    79261 aaggagattt agaggaatct tgtattgttg gtacattagg cgaattagtt tctgagaaaa
    79321 aaacatttta taaaggagaa tcgaggccga cgattttcga cgcagttggt ctagctattg
    79381 aagatctttg ttgtgcagaa tatatttata acaaattaga gaaaggggct ttaaaatgaa
    79441 cttagattat tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa atccagcagc
    79501 ggtttatgtg ctagaagaat ggctgccaga aggtacgatg caaaaaattg ccattgaaaa
    79561 caatttatct gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt
    79621 tacgccagat cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag catttgtttt
    79681 atttaactat tacaaaatac ctgatgaaac gattaaattt tctactcaaa gcggtaatct
    79741 atttgtgaca agaattaaag attattatta catggatttt cctagtatta tgccgaaaaa
    79801 agtgccgata ttgtctgaat atgaagaagc gattggggct aagattaaag aagcctactt
    79861 agcacgtgat ttatttttcg tattagaaga tgaaaaaacg gtggctaaat tgcagccaga
    79921 ttttacagct atcaaaaatt ttgaattagg aataggagtc atcgtgacag cagaaagtgt
    79981 gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt
    80041 atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa
    80101 attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga
    80161 tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt
    80221 tctttcaaat gaattaaaaa taaattggtg gagagtggca accttatagc tacaatggtt
    80281 gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca ttttaattat
    80341 tggtgtttgt tttttattca atcaagagta aaatgcccca tactaactat cctcggatga
    80401 cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgca gtgccaggta
    80461 atccttttaa gtcagtaatc tcaagaccag cagctttatg ttgctcgtgt aatttttcta
    80521 gatcatcagc tgctatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat
    80581 catagttgta agtcaactct aattcatagt catccccagg aagtgtcaaa taaacaagtg
    80641 taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat
    80701 aaaattcgat tgatgcttca agatctttca ctcgaacaca tgtgtgagcc attttcatag
    80761 attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagga aatagttgat
    80821 ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataaaa ctgttaaggt
    80881 ggaaactatt catggataaa ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa
    80941 cccgttatgc agcatatatt gtcatagaaa gagatcagca gattgcagta atcgaggctc
    81001 caaatggtgc tttttttctt cctggcgggg aaattgaagg agaagagacg aaagaagaag
    81061 cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg
    81121 gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat
    81181 atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa
    81241 tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg
    81301 cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct
    81361 ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg
    81421 tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt
    81481 gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc
    81541 atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta
    81601 cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta
    81661 tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc
    81721 atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc
    81781 ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg
    81841 tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc catcacttcg
    81901 atctctagga aatcttcatg tgtcagtccg tgacgggcga tgattttacc tgatttcgtt
    81961 tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac
    82021 tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca
    82081 ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca
    82141 tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat
    82201 atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa
    82261 gtttcgtcaa ataaatcata atgatcacgc aaggacggat caaccatttc ttttggaaag
    82321 aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt
    82381 gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct
    82441 tgttctgggc gataaaaatc ataaggtaga tcgtagctag aattcacagc agtgtaataa
    82501 acaggattat atccgacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc
    82561 cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa
    82621 tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca
    82681 tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat
    82741 tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc
    82801 agatgctcca aaattacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac
    82861 atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg
    82921 atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat
    82981 tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga
    83041 ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc
    83101 ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa
    83161 gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga
    83221 gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc
    83281 tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg
    83341 cagattcgtc ttgaaatctc ataaacgcct aatgaatgag taaaacggct atgttctgct
    83401 ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg ttggacttct
    83461 ttcgaattga tcaaatcgaa gattacttga tgctggacat ggatatagtt atggactgga
    83521 tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag
    83581 ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg
    83641 gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc
    83701 aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc
    83761 ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc
    83821 ttttaaagca gattgataaa atcgcttcat gacttgcccg cgtttttcct gattaccgtt
    83881 gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc
    83941 aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc
    84001 gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta
    84061 agcgtcatct atggttattt tttttgtgtc actttggggg aaaatcaaag aaatattcaa
    84121 aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc
    84181 tgcatctttc agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc
    84241 taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga
    84301 atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg
    84361 ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc
    84421 tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg
    84481 gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga
    84541 gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta
    84601 aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt
    84661 ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct
    84721 gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg
    84781 atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt
    84841 atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca
    84901 gcagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat
    84961 tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga
    85021 ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt
    85081 gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa
    85141 cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgggaaa
    85201 tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc
    85261 agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg
    85321 atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt
    85381 aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac
    85441 gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat
    85501 gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat
    85561 gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata
    85621 gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa
    85681 taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag
    85741 gtgtgacacg atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat
    85801 aaaaaataaa aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa
    85861 aggagtggag aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc
    85921 acctgctgaa tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt
    85981 acgtacatga taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccctga
    86041 actattttga aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga
    86101 tccgcacggc attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa
    86161 ttcaagatat cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt
    86221 tagataaaat agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc
    86281 gtttgaaaga aattgcgact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt
    86341 accgctcatt gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat
    86401 tcccaaaaag aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa
    86461 gggcttaaag aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca
    86521 aagcggagaa agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca
    86581 acaagtagcc ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta
    86641 ggatcagtca ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat
    86701 attcaggtac cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta
    86761 aatcaaaaat actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga
    86821 aattttgaaa cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac
    86881 cggtttctcg tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa
    86941 agatacgaca caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa
    87001 gatgacacct atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc
    87061 aaggaagatc catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa
    87121 aaaccaacac ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt
    87181 ttgacttttt ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt
    87241 cctaagtccg tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga
    87301 agtagttgcg actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa
    87361 aaagagaaag aagagatata gtgaaaagaa attatttagt tatcagtatt attgctttat
    87421 ccgtgctttt gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac
    87481 aaacacactc ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta
    87541 aaagccaatc aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg
    87601 tacaaagtca gtcatcacaa tcaacacaag acagtacaaa aacgagccga gatccggctg
    87661 tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc gaccacaaca ccgcagcttc
    87721 tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga gaaaatacgg
    87781 cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca
    87841 catacgatca tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga
    87901 aagcagacac tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt cgtccgccct
    87961 atggttctgt cacgaatgta ggagcaacga tcatccagcg accgatcatc gaatggtctg
    88021 ttgattcaga agattggaag acaagaaatc ctgatttgat tttgcagaag atccaagcaa
    88081 cagtatacga tggggcaatt attttattcc atgatatcta tcctgaaacg attcgtgccg
    88141 ttcctcaagt gatcgattat ctgaaagagc aagggtatcg catcacgaca gttggggatt
    88201 tattagggca tccaacggct gtcgagaact attatggcag aaacgatcat cggccagttc
    88261 agtaagtgag acaagtatct ttaataaata acacatttta ataagtagtt gaacacataa
    88321 ctaccttact tgcaaaggct taacaatatc tatacaggta caacaaaaaa ctgtccacca
    88381 attttctttg gcggacagtt ttcacttaaa aataaagaag caactattcg taaattatgc
    88441 taaaatagca gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga accggaatct
    88501 tacgtgatat ccactacact aaggacgcaa gcactgggat aattgtaact aaacagataa
    88561 aaaattacaa ggggattgga caaaaaaatg aaatttgagc aaaatttttc acaaaaacag
    88621 cagcagacac agaaactagc catgacgcaa cagttgcaac agtccatcca agttttacaa
    88681 tataacagtg aggagctgtt ggcattcgtt gagaatcaag caatggaaaa tccattggtg
    88741 gaagttgttg agccggaatg gcagccagat tacataaaag cttcttcatc ttcttatgaa
    88801 ggagaagaga caaattacct aaatcagatt cctgatacaa aagggtcttt atttgattca
    88861 ttgatcgaac aagtccattt gaattaccga gatacttttt tgaggaaaat cgttctttat
    88921 cttgtagaat acatcgattt gaacggattt ttgacgatta gtttagaaga agccggaaaa
    88981 gagattggag ctacgcctat ccaaatgctc gatgcgttga ctctgattca gcaattagaa
    89041 ccagctggag taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac agaacgagat
    89101 gattatgcgc cggaattagc ttatatcgtg ttggaagaat gttttgagga actggtggag
    89161 cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc tacatgctgt tcagcagata
    89221 ttcgactatt tacaaacgtt gagtccaagc cctggccgga ttttcgaccg tagcagcgaa
    89281 ttgtacatta ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt acaagttatc
    89341 tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga gttacttcaa acaaatgtca
    89401 caacaagcag ataaagaaac tgaaaattat ttaaaagaac ggaaacagga attcgaatgg
    89461 ttgaagaaaa cgatcctaca acgtggggat acaattttgc gggttgctca agtcattgtt
    89521 tcccggcaaa aagctttctt tatcgacaag gaacgtccaa tcaagcctct tacgttaaag
    89581 gaagttgcaa ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa
    89641 tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac acgaattcct
    89701 acgaatagta cagaacagac agaagacctt tcagcagata cagcgaagaa aaagctgcag
    89761 gaattagtcg atcaagaaga caaaaacaag ccgttgtctg accagaaatt ggttgaattg
    89821 ctgaaaaaag atgagatagc gatctcaaga cgaacagtag caaaatatcg tgatttatta
    89881 ggaatcccga gttcatcaaa aagaaaacgt tatgataact aatcagtcaa aagactagaa
    89941 aaagtatttc actttttcta gtctttttag ttgaaaaacg agagcaaaac tgttagactt
    90001 acagtcgtga ggttgagatt tccctttatt gatgtgaaat tcgaatagtt aatttctttt
    90061 tttatcgtat atgggtcgtt ttaagtctac attggacgta aagtgtccaa ctaaggagat
    90121 cgctatgctg gatgaaataa aaatgattga atctgttgca ccagatatta tcgaagtgtt
    90181 gcaagaacgc tataaaattt tacgaaatat ctattggatg cagccgattg gacgtcgaag
    90241 tttatcggaa agtatgggga tcacagaacg agtattgcga acagaaacag atcttttgaa
    90301 aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta agggcgaaga
    90361 agtttatcaa agccttgaaa acttcatgga tcagctttta ggtacgcatc aaaccgaaca
    90421 acagttggca gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga
    90481 gcagaccaaa gtggctgatg cattcggcga agcgctgagc gaagccctag accgactact
    90541 tccagaaggc gagaatatca tcgctgtgat gggaggaact actatggctg tggtagccga
    90601 acaattgtct aatctggaaa ataagaaacg gcataacctg tttgtccctg caagaggcgg
    90661 gattggtgaa gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc
    90721 aaacggtaac caccgtgcac tttatgttcc ggaacaatta agtctggcaa cttacaattc
    90781 tttactcaat gagccatcta tccaagaagt cttaaacttg attagtgaag cgaattgtgt
    90841 tattcatagt attgggcgtg cattacacat ggcagcacgt cgtaagatga ctgaaaaaga
    90901 attagtcatg ctgaaacaag caaatgctgt tgcggaatct tttggctact ttttcaatga
    90961 aaaaggtgaa gtcgtttata aggtccctag aatcggcata gaattgcaag atttggaaaa
    91021 agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta
    91081 tatgaaaaat gcaccaaaac aaacgtggct catcactgat gaagctgctg caaatgagat
    91141 tttaaaaggg gtaacccttt aaaataaata tttttttgat tttcataagg aggaaatctt
    91201 aaatgacagt taaagtaggt attaatggtt ttggacgtat tggacgtcta gcattccgtc
    91261 gtatccaaga tgtagatgga atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa
    91321 tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga acagttgaag
    91381 ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa agtattagct aaccgcaacc
    91441 cagaagaatt accatggggc gaactaggcg tagatatcgt tctagaatgt actggtttct
    91501 tcacttctaa atcagcagct gaaaaacatt taacagctgg tgctaaacgc gttgttatct
    91561 ctgctcctgg cggaaacgat gttccaacaa tcgtttataa cacaaaccac gaaacattaa
    91621 ctggtaaaga aacagttatc tcaggtgctt catgtactac taactgttta gctcctatgg
    91681 ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt aatgacaact atccacgctt
    91741 acacaggtga ccaaatgact ctagacggac ctcatcctaa aggtgacttc cgccgtgcac
    91801 gcgctgctgc tgcaaacatc gttcctaact caacaggtgc tgctaaagct atcggtttag
    91861 taattcctga attgaacggt aaattagacg gagctgctca acgtgttcct gtaccaacag
    91921 gttcattaac agaattagta acagttcttg aaaaagaagt aactgttgac gaaatcaatg
    91981 cagcaatgaa agaagcttca aacgaatctt acggatacaa cacagacgaa atcgtttctt
    92041 cagatatcgt tggtatgact tacggttcat tatttgatgc tacacaaact aaagtaatga
    92101 cagttggcga caaacaatta gttaaaactg ttgcttggta cgacaacgaa atgtcataca
    92161 ctgcacaatt agtacgtact ttagaatact tcgctaactt ataagataga caaacgtaca
    92221 agtgtaaatt atagacatgt acatttaaaa gcggggaagc aatcgcgctt cttccgcttt
    92281 ttttgataat aagtttattc aggaggaaat aaaatggcta agaaaacagt gaaagatatc
    92341 gatttaaaag acaaaaaagt tcttgtccgt gttgacttca acgttccttt gaaagacggc
    92401 gtgatcactg acgacactcg tatcaaagca gcattgccaa caatcaacta cgttctagaa
    92461 caaggcggaa aagcaatcct tttctctcac cttggacgtg taaaaacaga agaagataaa
    92521 gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg aattattagg taaagaagta
    92581 acattcgtac ctgaaactcg cggagaacaa ttagaagaag caatccgcaa catgaatgac
    92641 ggcgacgttg tcgtattcga aaatacacga tttgaagata ttgacggtaa aaaagaaagc
    92701 aaaaatgata cagaactagg taaatactgg gcttcattag gcgatgtatt tgttaacgat
    92761 gctttcggta cagctcaccg tgcacatgct tctaacgtag gtatcgcttc aactggtatc
    92821 ccaacagttg ctggattctt gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa
    92881 gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc
    92941 gtaatcgaaa acttgattcc taaagcagat aaaatcttga tcggtggcgg aatgacttac
    93001 acattctacg aagcaaaagg aatcaagatc ggtaactctc tagtagaagc tgataaagtg
    93061 gaactagcaa aagaattaat cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat
    93121 gtctgtgcac cagaattttc aaatgatgtt gaaacacaag taatcgaagg agacattcct
    93181 gatggtttga tggctttaga catcggccca gcatcagtaa aattatttgc tgatacatta
    93241 caaggtgcaa aaacagttgt ttggaacgga cctatgggtg tatttgaaat gagtaacttt
    93301 gctaaaggta caatcggtgt ttgtgaagca atcgctaact tagaaggcgc aacaacaatc
    93361 atcggtggcg gagactcagc cgcagcagca gaacaattag gatttgctga caaattcaca
    93421 cacatctcaa caggtggcgg cgcaagcttg gaattgcttg aaggtaaaga attaccagga
    93481 ctagcagcaa tcaacgataa ataaacgacc aagtttgctc aattctgacg agtaatcgaa
    93541 caaaaggtga agggagtttt catgactccc tagccatttt tcaataaatt aaggagtgtg
    93601 ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga
    93661 agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct tcaaatgaca aagttgattc
    93721 agtcatcgga tctcctgcat tattcttaca agaattaatg acatcagctg aaggtacaga
    93781 actaaaaatc tcagcacaaa actgctactg ggaaaactca ggtgcgttca ctggtgaaac
    93841 ttcaccagca gctcttgctg atctaggtgt tcattatgtg atcatcggac attcagaacg
    93901 tcgtgaatat ttccatgaaa cagacgaaga catcaataaa aaagcgaaag ctatctttgc
    93961 aaatggtatg acaccaatcc tatgctgtgg cgaatcttta gaaacctatg aagcaggcaa
    94021 aactgctgaa tggatcgaag gacaaattac agctggttta gctgatcttt cagctgaaca
    94081 ggtgtcaaac atggttattg cttatgaacc aatctgggca atcggtactg gtaaatcagc
    94141 tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt caaacagttg aaaaacttta
    94201 tggaaaagaa gtatcagaag cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa
    94261 tattgctgaa tacatggcaa aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt
    94321 gcaagcagat tcattcttgt cattgttaga cgctgtaaaa taattgttga aaatttgcga
    94381 aaaatgcaag aaatgtttgc actaatgagc aaaattaact aaaatcgtat atgagggaaa
    94441 tcccttagga aaaaacaaac tcaaaggaga gacaaaacat gtcaattatt actgatgttt
    94501 acgctcgcga gatcttagac tcacgtggta acccaacaat cgaagtagaa gtatacacag
    94561 aaagcggagc ttttggccgc ggaatggttc cttcaggtgc ttcaactggt gaatacgaag
    94621 cagttgaatt acgtgacgga gacaaagctc gttatggcgg taaaggtgta actaaagctg
    94681 tcgacaacgt aaacaacatc attgctgaag caatcattgg ctacgatgtt cgtgaccaaa
    94741 tggctatcga caaagctatg atcgctttag acggaactcc taacaaaggt aaattaggtg
    94801 cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc agctgctgac tacctagaag
    94861 tacctttata ccactaccta ggcggattca acacaaaagt attgccaaca ccaatgatga
    94921 acatcatcaa cggcggatca catgctgata actcaatcga cttccaagaa ttcatgatca
    94981 tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat gggtgctgaa gtattccacg
    95041 cattagcttc aatcttgaaa gctcgcggat tagcaacttc tgttggtgac gaaggtggat
    95101 ttgctccaaa ccttggttca aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa
    95161 aagctggcta tgtacctggt aaagacgttg ttcttgctat ggatgctgct tcttcagaat
    95221 tctacgacaa agaaaaaggt gtttacgtac tagctgattc aggcgaaggc gaaaaaacaa
    95281 ctgacgaaat gatcaaattc tacgaagaat tagtttctaa atatccaatc atctcaatcg
    95341 aagacggatt agacgaaaac gactgggacg gattcaagaa attaactgac gtattaggcg
    95401 acaaagttca attagttggt gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag
    95461 gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa
    95521 cagaaacttt cgaagctatc gaaatggcta aagaagctgg ctacacagca gttgtatctc
    95581 accgttctgg tgaaacagaa gattcaacaa tctctgatat cgctgttgca acaaacgctg
    95641 gccaaatcaa aactggttct ctatcacgta ctgaccgtat tgctaaatac aaccaattac
    95701 tacgtatcga agaccaactt ggtgaagttg cagaatacaa aggtttgaaa tctttctaca
    95761 acttaaaaaa caaataattc ttttctaatg aagatacatg atcgactatt gaaagatagt
    95821 cgagtatgat cacttagtta agtaatcgaa ctctctactt gctgaaatat cagcaggtag
    95881 agagtttttt tgcaactagc ttctgtatat gatgtttaca aagaatggta caagaaaaga
    95941 tcatccttga aaaaggaaag ctaacaataa ctagcatcat ataagagctt ttaaggatag
    96001 aacagtagaa taggaatcta tgagcaagtc ctctcgtata aataaaatag gagggaagta
    96061 gcagcatatt tttcataaag tggctctctt tcttcatttt tactttggtt gctcctattt
    96121 gaaaattcga gcacactcaa atacaataaa aagaaatggc aagggagagg attagatgaa
    96181 aaaagaagaa aaaatcgcga ttttacaaga gatcatccga attaaatcag tgaatggaaa
    96241 cgaaggtgaa gtagcagcct atttgaataa gcttttggca aagcatgaca taacaggaga
    96301 gattgtttct tacagagatg gtagagataa tttgattgct cgttaccaaa agggccaatc
    96361 aggaaaagtg ttaggcctat ctgggcatat ggatgtcgtg gcggctggtg atgaatcgtc
    96421 ttggacctat gcaccctttg cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc
    96481 tacagatatg aagagtggct tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc
    96541 aggaaaacca tttaacggaa cagtcaaatt attagccact gtcggagaag aagtgggtga
    96601 actaggtggg gaacagctca cgaaagcagg ctatgtagat gacttagatg ccttgatcat
    96661 cggagagcca accaattaca gtttgatgta tacgcacatg ggatcaatca actatacagt
    96721 cacctcgcac ggaaaagaag cgcatagctc tatgccagac caaggttata atgcaatcaa
    96781 tcacctaaat gaattcatta cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat
    96841 cgagaatcct gtcctaggaa aaacaatcca taatgttaca ttgatcagtg gcgggaatca
    96901 agtcaacagc atcccaagcc atgcgcagct ccaagggaac attcgatcga ttccagaata
    96961 cccaaatgat aaaatcattg cattgctgca atcaatcgtc aatgaattga atcaagagac
    97021 agactatcat ttggaattga tgatcgatta caacaaaata cctgtcaaag ctgatccaga
    97081 ttctcctttg attcattgca ttcagcaaca attcagccag ccattacctt tagttggagc
    97141 ggcagcaaca acggatgcag ccgaatttac gaaagctaac cattccttcg actttgtcgt
    97201 ctttgggcct ggtgtcgtta ctttgcctca tcaagtagat gagtatgttg aaatcgataa
    97261 ttatttggat atgatcgaaa aatatcaagg aattatttta tcctacttag cgtaagattt
    97321 ttttaataaa tgaaaaaaca gagccagatt agcatt
//
LOCUS       DACCWV010000002        77976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCWV010000002 DACCWV010000000
VERSION     DACCWV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.002"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            20..697
                     /locus_tag="IVW84_000099"
     CDS             20..697
                     /locus_tag="IVW84_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916332.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            700..1827
                     /locus_tag="IVW84_000100"
     CDS             700..1827
                     /locus_tag="IVW84_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916333.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2027..2467)
                     /locus_tag="IVW84_000101"
     CDS             complement(2027..2467)
                     /locus_tag="IVW84_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7916334.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2478..4511)
                     /locus_tag="IVW84_000102"
     CDS             complement(2478..4511)
                     /locus_tag="IVW84_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7916335.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4513..6216)
                     /locus_tag="IVW84_000103"
     CDS             complement(4513..6216)
                     /locus_tag="IVW84_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7916336.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6310..7260)
                     /gene="manA"
                     /locus_tag="IVW84_000104"
     CDS             complement(6310..7260)
                     /gene="manA"
                     /locus_tag="IVW84_000104"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7916337.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7299..8324)
                     /locus_tag="IVW84_000105"
     CDS             complement(7299..8324)
                     /locus_tag="IVW84_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7916338.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8533..9402)
                     /locus_tag="IVW84_000106"
     CDS             complement(8533..9402)
                     /locus_tag="IVW84_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7916339.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9861..11753)
                     /locus_tag="IVW84_000107"
     CDS             complement(9861..11753)
                     /locus_tag="IVW84_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916340.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11750..13477)
                     /locus_tag="IVW84_000108"
     CDS             complement(11750..13477)
                     /locus_tag="IVW84_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916341.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13493..14143)
                     /locus_tag="IVW84_000109"
     CDS             complement(13493..14143)
                     /locus_tag="IVW84_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7916342.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14347..14643
                     /locus_tag="IVW84_000110"
     CDS             14347..14643
                     /locus_tag="IVW84_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7916343.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14815..15435
                     /locus_tag="IVW84_000111"
     CDS             14815..15435
                     /locus_tag="IVW84_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7916344.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15527..16693)
                     /locus_tag="IVW84_000112"
     CDS             complement(15527..16693)
                     /locus_tag="IVW84_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7916345.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16705..17058)
                     /locus_tag="IVW84_000113"
     CDS             complement(16705..17058)
                     /locus_tag="IVW84_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7916346.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17186..18208
                     /locus_tag="IVW84_000114"
     CDS             17186..18208
                     /locus_tag="IVW84_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7916347.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(18647..19081)
                     /locus_tag="IVW84_000115"
     CDS             complement(18647..19081)
                     /locus_tag="IVW84_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916348.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19191..21050)
                     /locus_tag="IVW84_000116"
     CDS             complement(19191..21050)
                     /locus_tag="IVW84_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7916349.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21168..22328)
                     /locus_tag="IVW84_000117"
     CDS             complement(21168..22328)
                     /locus_tag="IVW84_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7916350.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22635..23546)
                     /locus_tag="IVW84_000118"
                     /pseudo
     CDS             complement(22635..23546)
                     /locus_tag="IVW84_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(23712..24905)
                     /locus_tag="IVW84_000119"
     CDS             complement(23712..24905)
                     /locus_tag="IVW84_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7916351.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(24989..25192)
                     /locus_tag="IVW84_000120"
     CDS             complement(24989..25192)
                     /locus_tag="IVW84_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7916352.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            25449..25691
                     /locus_tag="IVW84_000121"
     CDS             25449..25691
                     /locus_tag="IVW84_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916353.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(25613..25846)
                     /locus_tag="IVW84_000122"
     CDS             complement(25613..25846)
                     /locus_tag="IVW84_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7916354.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(25853..26284)
                     /locus_tag="IVW84_000123"
     CDS             complement(25853..26284)
                     /locus_tag="IVW84_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7916355.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            complement(26872..27480)
                     /locus_tag="IVW84_000124"
     CDS             complement(26872..27480)
                     /locus_tag="IVW84_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7916356.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(27486..28514)
                     /gene="vanB"
                     /locus_tag="IVW84_000125"
     CDS             complement(27486..28514)
                     /gene="vanB"
                     /locus_tag="IVW84_000125"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7916357.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(28507..29478)
                     /locus_tag="IVW84_000126"
     CDS             complement(28507..29478)
                     /locus_tag="IVW84_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7916358.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(29475..30302)
                     /gene="vanW"
                     /locus_tag="IVW84_000127"
     CDS             complement(29475..30302)
                     /gene="vanW"
                     /locus_tag="IVW84_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7916359.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(30320..31126)
                     /gene="vanY"
                     /locus_tag="IVW84_000128"
     CDS             complement(30320..31126)
                     /gene="vanY"
                     /locus_tag="IVW84_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7916360.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(31297..32640)
                     /gene="vanS"
                     /locus_tag="IVW84_000129"
     CDS             complement(31297..32640)
                     /gene="vanS"
                     /locus_tag="IVW84_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7916361.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(32643..33302)
                     /locus_tag="IVW84_000130"
     CDS             complement(32643..33302)
                     /locus_tag="IVW84_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7916362.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(34245..34430)
                     /locus_tag="IVW84_000131"
     CDS             complement(34245..34430)
                     /locus_tag="IVW84_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7916363.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            34575..34946
                     /locus_tag="IVW84_000132"
     CDS             34575..34946
                     /locus_tag="IVW84_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7916364.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            35204..35533
                     /locus_tag="IVW84_000133"
     CDS             35204..35533
                     /locus_tag="IVW84_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7916365.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            35494..36822
                     /locus_tag="IVW84_000134"
     CDS             35494..36822
                     /locus_tag="IVW84_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7916366.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            36819..37190
                     /locus_tag="IVW84_000135"
     CDS             36819..37190
                     /locus_tag="IVW84_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916367.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            37373..37609
                     /locus_tag="IVW84_000136"
     CDS             37373..37609
                     /locus_tag="IVW84_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7916368.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(37909..38112)
                     /locus_tag="IVW84_000137"
     CDS             complement(37909..38112)
                     /locus_tag="IVW84_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916369.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(38116..39060)
                     /locus_tag="IVW84_000138"
     CDS             complement(38116..39060)
                     /locus_tag="IVW84_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916370.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(39061..42981)
                     /locus_tag="IVW84_000139"
     CDS             complement(39061..42981)
                     /locus_tag="IVW84_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7916371.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(43130..45211)
                     /gene="topB"
                     /locus_tag="IVW84_000140"
     CDS             complement(43130..45211)
                     /gene="topB"
                     /locus_tag="IVW84_000140"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7916372.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(45208..46437)
                     /locus_tag="IVW84_000141"
     CDS             complement(45208..46437)
                     /locus_tag="IVW84_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7916373.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(46427..46678)
                     /locus_tag="IVW84_000142"
     CDS             complement(46427..46678)
                     /locus_tag="IVW84_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7916374.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(46701..48692)
                     /locus_tag="IVW84_000143"
     CDS             complement(46701..48692)
                     /locus_tag="IVW84_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7916375.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(48724..51123)
                     /locus_tag="IVW84_000144"
     CDS             complement(48724..51123)
                     /locus_tag="IVW84_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7916376.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(51053..51475)
                     /locus_tag="IVW84_000145"
     CDS             complement(51053..51475)
                     /locus_tag="IVW84_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7916377.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(51489..52031)
                     /locus_tag="IVW84_000146"
     CDS             complement(51489..52031)
                     /locus_tag="IVW84_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7916378.1"
                     /translation="MLISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            complement(52062..52925)
                     /locus_tag="IVW84_000147"
     CDS             complement(52062..52925)
                     /locus_tag="IVW84_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916379.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(53017..53232)
                     /locus_tag="IVW84_000148"
     CDS             complement(53017..53232)
                     /locus_tag="IVW84_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7916380.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(53309..55117)
                     /locus_tag="IVW84_000149"
     CDS             complement(53309..55117)
                     /locus_tag="IVW84_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7916381.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(55114..55590)
                     /locus_tag="IVW84_000150"
     CDS             complement(55114..55590)
                     /locus_tag="IVW84_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7916382.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(55665..55877)
                     /locus_tag="IVW84_000151"
     CDS             complement(55665..55877)
                     /locus_tag="IVW84_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7916383.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(55899..57098)
                     /locus_tag="IVW84_000152"
     CDS             complement(55899..57098)
                     /locus_tag="IVW84_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7916384.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            complement(57355..57876)
                     /locus_tag="IVW84_000153"
                     /pseudo
     CDS             complement(57355..57876)
                     /locus_tag="IVW84_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(57892..58590)
                     /locus_tag="IVW84_000154"
     CDS             complement(57892..58590)
                     /locus_tag="IVW84_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7916385.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(58547..60208)
                     /locus_tag="IVW84_000155"
     CDS             complement(58547..60208)
                     /locus_tag="IVW84_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7916386.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            60426..61826
                     /locus_tag="IVW84_000156"
     CDS             60426..61826
                     /locus_tag="IVW84_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7916387.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(62078..63157)
                     /locus_tag="IVW84_000157"
     CDS             complement(62078..63157)
                     /locus_tag="IVW84_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7916388.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(63243..64202)
                     /locus_tag="IVW84_000158"
     CDS             complement(63243..64202)
                     /locus_tag="IVW84_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7916389.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(64214..65737)
                     /locus_tag="IVW84_000159"
     CDS             complement(64214..65737)
                     /locus_tag="IVW84_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7916390.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(65747..66310)
                     /locus_tag="IVW84_000160"
     CDS             complement(65747..66310)
                     /locus_tag="IVW84_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7916391.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(66316..67179)
                     /locus_tag="IVW84_000161"
     CDS             complement(66316..67179)
                     /locus_tag="IVW84_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7916392.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(67193..68089)
                     /locus_tag="IVW84_000162"
     CDS             complement(67193..68089)
                     /locus_tag="IVW84_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916393.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(68264..69535)
                     /locus_tag="IVW84_000163"
     CDS             complement(68264..69535)
                     /locus_tag="IVW84_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7916394.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(69525..71042)
                     /locus_tag="IVW84_000164"
     CDS             complement(69525..71042)
                     /locus_tag="IVW84_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7916395.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            71240..72130
                     /locus_tag="IVW84_000165"
     CDS             71240..72130
                     /locus_tag="IVW84_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7916396.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(72203..73027)
                     /gene="nadE"
                     /locus_tag="IVW84_000166"
     CDS             complement(72203..73027)
                     /gene="nadE"
                     /locus_tag="IVW84_000166"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7916397.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(73039..74511)
                     /locus_tag="IVW84_000167"
     CDS             complement(73039..74511)
                     /locus_tag="IVW84_000167"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7916398.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(75148..75510)
                     /locus_tag="IVW84_000168"
     CDS             complement(75148..75510)
                     /locus_tag="IVW84_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7916399.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            75733..76485
                     /locus_tag="IVW84_000169"
     CDS             75733..76485
                     /locus_tag="IVW84_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7916400.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(76625..77548)
                     /locus_tag="IVW84_000170"
     CDS             complement(76625..77548)
                     /locus_tag="IVW84_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7916401.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(77563..77835)
                     /locus_tag="IVW84_000171"
     CDS             complement(77563..77835)
                     /locus_tag="IVW84_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916402.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        20272 a        18370 c        16274 g        23060 t
ORIGIN      
        1 ttatattgga ggcctatcta tgacattact gatcaaaaac atgaacatga aacgtggtaa
       61 aaaacgtgtg attcgacaat tgaatctttt agtagaatca ggagaagtag ctgctttgat
      121 tgccccgaac ggatttggca aaacaacttt gctgaatggc attgcccaac tgctgccaac
      181 aacttatgat caattgtccc ttcacaacat ctctcctact gcttatacag attatcgccg
      241 gtctttcttt tttctagaat caacgaatca gttatccgac tatctatcgg ctgttgatta
      301 tttgactttg atcaaatact attggcagag caaagtagaa tgccaaactg ttttccaacg
      361 attgaagatg actgagtacc ttactttgcc gattggaaaa atgtctctag gaatgaaaca
      421 acatgtcttg cttgctgctt acttgatcag tgattcatct gtcatgttat tcgatgaacc
      481 gttgaacgga ttagacccag gaagtattga tatcttgaat agcctcatca gacaatggca
      541 gaaagagggg aagacgattc ttctttcttc ccacgctatc gcgaatatcc aatcgatttg
      601 ttctaaagca tgttttctaa aaaatggaga aatcgtcaca gaatcctcaa atatggaaga
      661 gattttagct atttatcgta aattatatga ggtgtaacta tgtctttact tacatttgaa
      721 cgcaatctct tactaaaaaa caaagtaaac tttttatttc ctctattgtt ggtcgtgctc
      781 tttgcattcc cacttttttt tgatcataaa cttgcttata cagaattcga tgaactgaat
      841 cataactatg aagagatgca acgtttgatt gaaacgttga aagaagatga gaatgagaaa
      901 gaatttgtgg aaagtttaga gaaaagcaac aaactgatcg aagcaatctt acatgcaaaa
      961 aacacaggga atgtccagca aatggtcgaa gcaacttatc attatgaaaa agatatactg
     1021 gatcgactga tttctggtca aagacaagga attccaatca ttgaacaaca aaaacgtgtc
     1081 gagttgctcc gttatatgaa agaacatcag atccaaagat attctatttt tgacttgcca
     1141 gcgcatctcc ctttagcaaa ttactacgaa aatatttttt caggaatgat ttctagtttt
     1201 ttgattttat gtataaccgc cttgtttctt tcttccatca tctcttatga aaaaagaaag
     1261 caggtaatct ctctagtcaa tctattaccg gatagtatgg taaagaaaca tagcatccgt
     1321 tttaccattt attatggtgc agctatgcta agtctcgtga tgccattttt gatcgtctct
     1381 atactagtaa taatcaaaaa tggtttggga gacttccgtt atcctgtagg aacgatcatt
     1441 ggtcaggaga tacgaatcct tccgatgtat gagtatcttt tccagagctt tctgttcctg
     1501 ttgttgtggg tgctcttttt aagtacaatc agttttcttc tatctgcttt atttgaacat
     1561 tctctagtca atcttttagg tacacttctt tgtcttttct tagcagagta tcgcttgttt
     1621 tcttcgattg gatggataga atcaatcagt cattacttgc caacttctta tgttgatttt
     1681 caaaatgtga tcataggagg agatattttc tcgccactag cttcagaaca ggttactttc
     1741 atgaatggta tcttgacact tggtatatgg agtatcgttt tattattcat aggtatgggc
     1801 actatctata taaaaaagag ctattaaaac acaaaaataa taggggcggt gacaaaaacc
     1861 ttgtcaccgt cccctatttc aaataaacgg ttttctagaa taaatagttt tcaaaagctt
     1921 gcatccgcag taataagaac ctcccaacaa aaatagctcc tcttattttt atcttggttg
     1981 aagttattac ttgatgcaag gcagcttttt ttactgtgaa cattggttat atatgtccat
     2041 gttcaaaaat catttggact tcttcaatgg ttttcgcatc actgagcgcc attacttggt
     2101 cgatgtctga acagaaaaag gctaaatctt ggagcaccgc taactgatgt tcttttttta
     2161 aagccactac aaataagagt gtcgcgattt ttggttcctc ttttgttcca aagtccacac
     2221 catcaggcac ttggactaaa tagattcctt cttctactac ttcttcatct ttgtcagtcc
     2281 catgtggcaa ggctacgaaa ttcccgatat agacactcgc tttttcttgt ctttcgatca
     2341 ttgattggat atatgccggt ttgattcccc cttgttgggt catccactgg ccgatctcgg
     2401 tcaacgcttc tttccaattt tgataggatt gatcgagcaa tattttttct gctggtagct
     2461 gtatcatctt ttcaccttca tttctttatt tcttcaatca ggattttcgc taacaactga
     2521 taaacgattt ctttatttcc tgagcggaag atctcggtgt tcaagtcatt catgatgatt
     2581 gctccactga tctttcctaa aagaatcccg tctttttctt ggattggcga aggcgccagc
     2641 ataactaaca tacgggacaa atcgactggc tgtctatcca tcccttcaat ggtcaacgga
     2701 tgtttcaaat tgaaaataca aaagatcggt ttagaaatca atgagctgga tgtatggaat
     2761 aaccccatat ttgtatgagg aataccgata ggtgcttgtt catatcgctt aagcagccgg
     2821 cgatgaacca cctcttcatc cgttactagt ccttcaggaa aataaggaaa taagctgcga
     2881 atcgtttcgg aaatcgtctc aggattatcc agttcttgga taaaaaaacg accgagtaat
     2941 tgattgattt gatccatgaa atgcattgtt tcctcataag tatcttctat catccactcg
     3001 ttattgctgt tatacgtaat tttttgcctt tttttcttct caatatgcga aaaagcttct
     3061 tttagctgct tgacttcatc ttctagcagt agcggggaga ctaaaatata ttttccaccg
     3121 tatcctggta aaatagatgt agaaataaca acatcatatt cttcttgtaa atcgattttt
     3181 tttaaatcag caatccgata gaaatgaatt tgttggatga agggaaaata ccttttcaat
     3241 cgtagttcca acatgctagt agaagctaat ccacttggcg aaaatcctgc aatactgata
     3301 ttgctttctt tcggacttcg ttccaatgaa ttcgcaaaat gaaggaccat ataagcgatc
     3361 tcttcttcgg ataatttttt ttcttgaaaa atccgctcta ctgctttacg gattgcattc
     3421 gcaacttcct cgtattgaac catgattttt tctaaaatcg gattggtcag tgtctcttct
     3481 tgtaaaacgg ctcttgaaaa cacaccagat aaatgagtca acagcatttt gtacaaagtt
     3541 tcatcttcat aaaaatggac attcgtctct tcacttactt cttgaatcag ttgttttact
     3601 aaataagcaa gctgtgtatc gaacgtctct tcaaagaaat tctgatcaaa tgagttcgtg
     3661 aagtcattta ataatcctgc aaaaaagacg atctcattta ctggataaag ttgttttgtc
     3721 tcagaagcga tccgtgaaaa caatcgttta gctgtctgta gtaattcttt gtttgccaga
     3781 ctattatatg tctcatttgt cagtaaatga tttttagtca cacgctccat cgtcaatgtc
     3841 agcatcaaaa ccaaatgttc aagctttcta tcacttagat tggtcttttt ttggatcaat
     3901 tcttccccga ttactttgcg tgcaagagat agatgttgcg gatcaacgaa tcgaaaaaaa
     3961 taattcagtg atgcatcgaa agaaatacta tgaaaaagtt catattcatt gatttctaat
     4021 tctaatacat tcgctgtcag aagccggcgg tatttttccg gtccttcgat ctcgtatccc
     4081 tggttccgaa tgatttccaa aggcagttgg cgaatacttt cttccaactg tttgatatct
     4141 gcataaaaag tggtattact gatgcgatat gattccatga aatgagcaag actcaacggc
     4201 tctgtcgtca gtaacaactc ggctaaaatc gcgtgttgtc gtttagcagt ggataagtca
     4261 ttacttttcc catctgtatt ttcttttaat tgctctaata cttcttctga tgcctcaatg
     4321 acaaatgagc ctctagctgc ttttttcaaa gaagcattta ccgatttcaa tgagacttca
     4381 agattatcta attcgcggta aatagtccgt ttactgacct tcaataaatt catcattttt
     4441 tggatagata caggtgacgg gctactgaga aattctgcta ataattgttt ttctctgggc
     4501 gataaataca tcttatattt ctcccttcac gagtttttcg tattcattcg tagctaccca
     4561 atgttcaact agaactagcc gagggatctt ttcttctagc tgttcagtta cagctagttc
     4621 tctttgtgcg atataaatcg tttcttcttg gattttgatt ttttctattg attcttttga
     4681 gataacaaac tgttttttcg ctttttttgc taattgactt aaaatcgtca tgcccatagt
     4741 ttgtgagccg cgtctgttgt ttttatacaa aaatacaata ttagcaggcg actttttttg
     4801 ttctgtgata tctgctatct cttttttttc ttcccgcttt ggaaattgga tcgcttcttt
     4861 tattcgtgac agcaagatat ctgtttccaa aaagtgatcc agtacataga tttgactttg
     4921 aggtactttt tctcttgcaa gacccgctag ttcatgttgc acgataacca gctgatttac
     4981 ttggttattc aattcataga tagagctgta tccgacagaa agatcgattt tttctgctgc
     5041 taatttcttt ttcagcaggg cggctcccat agcacttgat cccatacctg agtcgcaagc
     5101 aaaaatgacg gaatgaatga tagatggttc caagatttct cccctttctt tgaccgtttt
     5161 gttctctttt tctttatttg gtaatttcaa tagtatcgct gcacaaatga aggtcacaaa
     5221 tgtactgatt gctatcccta tacttacccc agctagttgt ggataaggcg cattggcaaa
     5281 aatcacgagt agtgacccag gcgatacagg tgcagctaat cccgtatcta accattgaaa
     5341 tatcaacgtg ccgcttgccc caccagccat tacagctaaa aatagatacg gtcgcagcaa
     5401 gacaaatgga aaatagattt cgtgaatccc gccaatcgca tggatcatca atgctccact
     5461 cgcatttact ttttcctgtt tttctgcaaa acaaaggata gaaagtaaga tccctaaacc
     5521 aggtccagga tttgcctcca tcaaaaataa ggaagagtac ccgactgttt gtgagaattc
     5581 gattcctaaa ggtgtcaata caccatgatt gattgcatta ttgaaaaata gtacttttag
     5641 aggttctaag atcagactga caagtggtaa cagattcatt gtgactagcc aagacacaac
     5701 ggaggcgagc aacaagtgaa cactatttaa aactggttca ataaaaacga ttccaattat
     5761 acaaataacg ctaccagtca gtcctactag taggttgctg acaagcattt catatccagc
     5821 cttgaatttc acgtggaatc tagaaacaaa ttgttcatat acgatgccgg caagcggccc
     5881 gataatcatc gctcctacga tttgtggagc attggaacga gcgatcaacc ctaaagaagc
     5941 aattgcaccg gcgactgccc ctttttgtcc ccctaccgat ttcccgcctg aataactgat
     6001 caagatgggt aaaagcagct ggatcatcca atggcatacc tcttctaatg tggtgtgcaa
     6061 atcgccagac acaccttttg ttgctaatga caagattccc caagcgataa acagacttag
     6121 atttggcatg atcatttcat ttaagtgatg tccagctttt ctcagttgtt tcatagacac
     6181 gcgactgctg ttgtttgctt tccatttgta ttccatcttt ttcaccatac ttctatttat
     6241 tgatggacgg attttatgta gaaaaagcag caaacgaaaa accgtctgct gctttcagct
     6301 atctgggaac tatttgcctg gttcagtcgc aatgattgct agttcacctt ggaccgccca
     6361 ctcagaaacg ccatttggaa taatacagct tgttcctttt tctaatggat atgtttttcc
     6421 gtcaacgaca aggtctcctg ctccttcgat taccgtcatc aatgtataag gggcttgttt
     6481 tttgaaagat gtgattcctt taatatccca ctcatataca ttgaaaaact cagtttctaa
     6541 ataagtgacg atcgatgaat tcccttttcg gacttctttg atttgtaatt ctggtgtttt
     6601 cgcagggaca gttgttacat cgatcgattg ttggatatgc agctctcttg tttgtccttg
     6661 cgcatctttt ctgtcgtagt catacacgcg gtaagtcgtg tcagaacttt gctgtgtttc
     6721 taaaatcatg attcctttac caatagcatg gatcgttccg cttggtacat aaaagaaatc
     6781 acctttttta actggtactt tcttcaaaag gtcatcccag cgcccatctt tgatcatctc
     6841 agctagttct tctcttgtct ttgcatgatg accatagatg atttctgcac caggttcagc
     6901 atcgatgata taccagcatt cagtttttcc taattcacct tcatgtttca tcccataggc
     6961 atcatctgga tgtacttgta cagatagatc atcttctgcg tctaagatct tgatcaataa
     7021 tggaaacact ggttctgaag gatggccaaa caattccttg tgttctgacc atagttcatc
     7081 taatcttttc cctttgaatt cgccattttc tactacactg acaccatgag gatgcgcact
     7141 gatcgcccaa tcttctccga ttttatcgtt agggatatca aaaccaaaga ctgaacgtaa
     7201 acgacttcct ccccaaattt tttcttgaaa aactggtttt aaaaacatag gttcctgcat
     7261 aattcaaact cctctcattt ttcatagcta ttatagcatt atttcggtaa taccgctttc
     7321 tctttcaaaa agttttcaac aatttcatct cgacatatga gatactccgc acgatgatcg
     7381 acaggatgta cgcgatttga caaaaatatc aacgcttctt gtgtttttat atcaatcaag
     7441 aaaaacgtac ctgtataacc agtatgaaaa agaatcgcat gaccgtcttc tacatccttt
     7501 tttaagtccc atcctaaaga acgatagcca ttttttttcg gtgtttgatc ttgcagtaaa
     7561 gaatcaatcg tctcttcttc taaaaaacga ccattacgga tttttccctt gttcaaatac
     7621 atttggacaa aagtaatgct gtctttcaaa tcaataaaga gacccgcgtt acctgcatgt
     7681 tcagccaaaa ctcttgcttt cggatcatga gtttgtccac gcaaaacctc acccgaaggc
     7741 agttgttctg tgggaacgat ttttttggaa ggaaaaggaa gaaataagct gttcatcatt
     7801 cctaatggtt ccaatacttg ttcttgaaag atttccactg cagtttcttg gtaaatttcc
     7861 tctagcatca acccaagaag aatcgtaccg gcatctgtat attttacttc ttttcctagt
     7921 tgctcacctg gttctagctt caaataagcg gcgatcaatt cttcctgaga tagcttatct
     7981 ctgtttggta tccatgtttt gatatcagaa gtatgtgtca gtaaatgacg caaagtgatt
     8041 gtctgatttt caaattcagg aagatattta tgaagcggtt gatcgagttc tatctttcca
     8101 tcttctaata gacgtaaaac taatgaagtg gttgcaacga ctttcgtcag tgaggctgca
     8161 tcaaacaaca gatcttctgt catcagctgc ttttcaggaa cgacttgggc aaagccggct
     8221 gtaaatgttt ctacttgatt ttcttttatg aaggcgactg ctacacctgg ataaaatcct
     8281 tcttccatct ttttcaaaat aagctgtttc gtgtgtggat acatcttttt ctcctgcttt
     8341 tctatcagat tcttacagtc caagcttaaa gcttaatcta tgaggaatca acctttttta
     8401 cacaaaaaaa ctgcgcgaaa aaccgcgcag tttcccccgg tatactattt tctctttcta
     8461 tctctagaat aagcgatagt atgaccgttc ataaaatgac gagacatgcg acaatcgtta
     8521 agaaagaaaa agttaagcgt cgcatcgaat gtcagtttta ttcttttctt ttggctcact
     8581 gacgaaccac cattcgattc ctatagcatc atagattgtg agtactgtgt gctttttgtc
     8641 taagaagaaa tcaactgata gttgctgcaa gtgagcaatc agtccttcca tgctttcagt
     8701 atcgatcatg aatttcagaa aatcaagtcc aatcacttca tctgtaggca aatcaattgc
     8761 gcctccgcta gcttctgtca aaccaacatg gaaatctcct tcattcaata catggatgcc
     8821 ctcttcttca ttatgtacct tcaaaccaag tacatgatta agaaattggc gttcttttgt
     8881 taagttagta acatttaaat ggattttttc aaaataagaa cctgttgata gtttactgaa
     8941 tttttcttca gattttctta ataatgcttc ttgatccatt gtttctggaa tttctctttg
     9001 gtcgttctta ttctcttcat ggtaaatttc caattcattc tcttctgggt ctacgagcaa
     9061 aatccccata cgtccatgat cttctaaagc ttctttgatt ggataattca ttttacggat
     9121 acgacaaagg atatctgcca tttcttctgt gctagggata atcaatgaaa aacggttcag
     9181 ctttttgatc acccccatgt gatcattcgc tctaggactt tcttccagca gcaacatttc
     9241 acttccagct tcttctgttc ctaaaatggc taattcgttt tcttctcttt tcaaatcaaa
     9301 gccaacaacg ttcttgtaaa aatcaatcat tttatctcgg tctttcaccc gaatggctac
     9361 actttttaaa taggtggcag cccccaattg aaaatctttc atatttcttg ctcccctcta
     9421 cgtcaagctt tattttaacg atggattctt cttctttcaa gcaataactt tgattttgtt
     9481 aaaaaaaatg tgagaaaatt cgcattttgc ttgttttttg taaattaatg agtaaaaact
     9541 cttataaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa
     9601 aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
     9661 caccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa
     9721 cggaaaaata gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt
     9781 gaacaccgtt tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa
     9841 aataataaat ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga
     9901 tctgcataga atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa
     9961 ccatgattca tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca
    10021 acgaaacttg tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt
    10081 cgtgtatcta cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat
    10141 gcccgcgcaa ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt
    10201 aatacagtgt tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta
    10261 gcagcctcaa tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag
    10321 atacttcctg taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca
    10381 tcacgtgaaa gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg
    10441 tagaaccgtt caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata
    10501 gcgaccatct caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt
    10561 gtgtagccga attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga
    10621 aggccagatt tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct
    10681 gaagcaactg ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag
    10741 aactgtgttg tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca
    10801 acttttactc cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc
    10861 aacggcatga tgattgccga aataaattga gctttccaac cagcggcata cagattctga
    10921 ttttcttttt caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta
    10981 tgaccaccat atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct
    11041 gcgaaaaaca cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc
    11101 atcaataacg cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca
    11161 atcaatgtga ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg
    11221 ttatccatat cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag
    11281 gaaatcggta cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt
    11341 tgggaaacac gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac
    11401 atcacgatta ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt
    11461 acttgttggc cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg
    11521 gttgctttcc ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt
    11581 acaaatacag cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc
    11641 gttccccaga aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca
    11701 tgtcctggac cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt
    11761 cctcctctct tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt
    11821 cttcatgtgt tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat
    11881 ccattactgt acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt
    11941 tcaatgcttg gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa
    12001 acacatagat atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc
    12061 caccagagaa gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg
    12121 aaacaaaatc tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat
    12181 ccggcttacc atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag
    12241 gaacaaagcc cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat
    12301 ttatttggat cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg
    12361 ttttacctga accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat
    12421 caatatcttc caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg
    12481 ccaatgttgc gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac
    12541 cgattttttc atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca
    12601 tgataaaaat agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa
    12661 atgcaatcag attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag
    12721 tgattgctac gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga
    12781 tcgtattaac tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt
    12841 cttcaaagcg tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa
    12901 ataccaggtt taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt
    12961 acatgattcc gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca
    13021 actgatgatc tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga
    13081 tcatcatacg taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc
    13141 ttgtgatcaa agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt
    13201 ttttatagac atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa
    13261 atgtatttgc gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg
    13321 tatgccaaat ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa
    13381 gtgtcggcaa atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg
    13441 ctgaagtcaa ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat
    13501 atactccgtt tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg
    13561 agtaatcttc agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat
    13621 tcatcactaa ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc
    13681 gatcgatcaa ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag
    13741 gcgttttttg gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca
    13801 taaacaagtt acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag
    13861 aaaaatagga ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca
    13921 tatctttaga gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat
    13981 cttcaaaata ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa
    14041 tcgaagcttc ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt
    14101 tttgtttttc tttaggcaaa tggaaaaatg tctgtgtcgg catatggatc ctcctttctt
    14161 tcaagatgac acattgtcat atgacgctgt gtcattataa cacctcctac ctgttttgca
    14221 agacatttgc atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat
    14281 ccttccattt gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg
    14341 agataaatgg cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac
    14401 agcatcgttt tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg
    14461 ctttctatcc ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt
    14521 tattatattc taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct
    14581 aatggttttc gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct
    14641 tgaatattgc ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt
    14701 tataccctgt ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct
    14761 tttctttttc tcatagttat aacaatcata gcaataaaag gagaaggtaa gcatatggca
    14821 tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac agttaaggca
    14881 ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt gactgttttg
    14941 gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg ttgggaagca
    15001 ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa aattgctcgt
    15061 ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc aaacgcctta
    15121 tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa tgtagctgga
    15181 aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa aaaagcactg
    15241 cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca atatgtaaaa
    15301 ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga aggaatcgat
    15361 tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc tgctgcttta
    15421 ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga aaaaatcttc
    15481 aaagcagcat ttgtaccttg gatattttcc catctgtctc tttttgttat tctttctgct
    15541 ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca aaaacatgag
    15601 taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg atgattccga
    15661 acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt gctaactgat
    15721 tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact gcaaaaacga
    15781 tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata gccggaatca
    15841 tcgctaaaaa tgatcctaag taagggatca ggttcaaaaa acccgcaata atccccaacg
    15901 tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt gccacagcaa
    15961 aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt tcactcagca
    16021 cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa ggtgctaatt
    16081 gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga gccactaaaa
    16141 tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta gatatatctt
    16201 ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac tgttcgcgaa
    16261 attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc gtatctacat
    16321 attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg atgatcacta
    16381 cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata ctgtaaagcc
    16441 ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa tacaagattc
    16501 ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt gcaaacaaat
    16561 atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca agtaaggctg
    16621 tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg ttcttcttat
    16681 tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca aaccaatagc
    16741 aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt ctggacaagc
    16801 atcaaaaatc aatgatacgt gagctactgt ctctaaattc ccattggcca tcggaccgac
    16861 ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt tcaaagaaaa
    16921 aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag ctgttgctgt
    16981 tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc caatttctgt
    17041 aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca gtataacaca
    17101 ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa aactatttca
    17161 ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa cctatacgcg
    17221 tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag aattagccaa
    17281 cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta aagctggcaa
    17341 tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg ataaggattt
    17401 tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag cggttgatga
    17461 agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg tctaccatat
    17521 attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag cgacaggtcc
    17581 tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg accaacgatt
    17641 agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg aaaacggcaa
    17701 attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc atcttgtctt
    17761 tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag tccgcgtgct
    17821 ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga ccttgccaga
    17881 agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg gcaagttcct
    17941 ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg aagatggttc
    18001 ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg atttcgcact
    18061 agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc tgacattata
    18121 tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct atgtaccaga
    18181 atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga atcagcaaga
    18241 cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga aaatcgaaga
    18301 taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga actactttga
    18361 acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa aagaacatca
    18421 ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac cgccgagatg
    18481 gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag gttgttgttg
    18541 gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag gtaatattac
    18601 atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat tttttcaaca
    18661 acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg tcttgttttg
    18721 cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata ctttggatca
    18781 tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta tgaatgccca
    18841 ttgcttttgc tggtggacgc aaagtactga ctttcttaag cattgcccga tatttttttg
    18901 ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt tcacttgttt
    18961 ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct tctccgattg
    19021 cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata ttcggtaaca
    19081 tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt tttatcctca
    19141 aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc ttattcgtgc
    19201 tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata ttggatcaat
    19261 tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt ataccactgt
    19321 ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa tgataaaagc
    19381 agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc tgcaaaataa
    19441 aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt actgtcgaac
    19501 ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat cgtatctgaa
    19561 tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga aagattcttc
    19621 aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg cagataattc
    19681 tctaagtcaa gtgtcccact tccatctgaa gtttttgcag gatagtctag attatcatac
    19741 tttccatcgt agggcatatg tgtctgcatc gtgactaaat gaatgaactg aggtgttttt
    19801 tcttccttga gaagtgtcat tacttctttg taagctgact catcagaaat atatggatta
    19861 ttctctatcg tatccgtata ggtcatcgta cgctcgctga tgaactggtc aaatcctaat
    19921 gtctgataaa catcctctct tttatacatc gaagtattgt atggatggat cgctgtcgtc
    19981 tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat ttttggtaca
    20041 agcatcgtgt aaggggtggt gagttgcgca ttaaataacg ccatagaaaa accagtcaat
    20101 gcttcaaatt cgatatttgc tgttccaccg ccatagtttt gagaaagcat attgccacta
    20161 tatgtttgat cagctacttc ataataatct gccaatggct caccagatac ttctacgccg
    20221 tttaatcgac taggatctga gaaactttca ctcatcacga acacgatgtt cggcgactct
    20281 tcctctacag ttttattttt ttcatcggct agcttctggt atttttcagt gatttctttg
    20341 atcttttctt tcgaatatcc ttcaggttca tccattggtt ctaccttcag attgtacaaa
    20401 aacccaccga taaaaccagt gttatagtag ttcattttct ggctgtaagg tatccatagt
    20461 gccgttttat tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt actgatatac
    20521 cctagcagtc caattgccac aagcagtgtc gttaatcgga tcaattggaa tcgccttcct
    20581 tttttcaggc ttcgaaacat gtaccagcaa aacaatccta tcacggttcc tgcggcagct
    20641 aagatcactg taaacagcat agtgccggtc atatctttca gcaatccgat ttcagtgatc
    20701 atcttcaaat cgtctggata aatcggttct tgccgataac tcattttcat ataatttgca
    20761 aaacctaata caccgatggc tacgacatac gtaagggaac ctaaagggac agatcctgct
    20821 agtgcaatca gaaaaataag aataatcagc aagacgagac aggctaagaa gaatttttcc
    20881 gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg atagatcatt ttgacaccac
    20941 tgcaaaaata aattactaaa aacaacaaga aaaaagatac tgatgattgc tgctgttcct
    21001 tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa atgcttgctc
    21061 cttcatttct atcaataaaa gaatgttgct tctcttattt tagttgtacg aaattcgaaa
    21121 gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc tctttcttta tggcctttct
    21181 ggtttcttta ataagcggac ctttcgtact ccagaaattt tttctagctg ttgtttcact
    21241 tgattgagct gttcaaaatc agtactgtct atgtcaatca atatgtagac atgctcattc
    21301 ttatggttac gcgatatatc tgcgatcgct agctcgttct cattcagcag agaaaatatc
    21361 tctgctaata tattggtttt cttttgataa aatatagtga atcgaaacgg cgcttggaaa
    21421 aacattcgtg cggatggata attgaccgct tctctaaccg taccgaacaa aagataatct
    21481 tttaaggctt caactgccaa acgtccgctg tctgccaaag cttcctgagt cgatccccct
    21541 agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc
    21601 aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt
    21661 tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga
    21721 tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta
    21781 gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa
    21841 taacccaaga catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct
    21901 ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt
    21961 ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct
    22021 tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta
    22081 gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg
    22141 atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca
    22201 tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg
    22261 aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg
    22321 tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact
    22381 gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat
    22441 aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag
    22501 ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc
    22561 aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt
    22621 ttcacaacct ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt
    22681 tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga
    22741 atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca
    22801 ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta
    22861 taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat
    22921 acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt
    22981 ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg
    23041 ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg
    23101 attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat
    23161 ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct
    23221 tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt
    23281 tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag
    23341 tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg
    23401 cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga
    23461 atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa
    23521 cgagccactt ttattccaaa attatatggg attttagatt atctttttag aagttcttga
    23581 atgtcggtat agcccgtgtt tccgggcttt tccggcattt ttgatattgt tagatactta
    23641 caaatgttct tattacctct caccatttcc ccctcaaaag tagtaaatgg agtagtaaaa
    23701 ccggccttgt gtcaggctgc cagccgttcc atttctgccc ttgcggaatc gaatgtggcg
    23761 tgtgcataat agttcagcgt catggtgata ttggaatgtc ccataatgta ctgcaaggct
    23821 ttggggttca ttcccgcatt ggctaaccgg gtgcagaatg tatgacgcat agcatggggc
    23881 gtcgtcgttt tcggcaacgc ttcttcatgg ctcttgttgt acttcttcac aagcctgcca
    23941 aacatcccgc cataatctac cgccgtcatg ggatagccgt tctgtttgag aaacaggaaa
    24001 ttggcatacc cgtcaataat gaacggcttt gcacctttgc ggttttttat cactcgctgg
    24061 aacgcctgat acacttcctc attcatgggg atttttctga cgccgctttg ggtcttgggt
    24121 tcgtcaatat agtaaccgac gccggtattt ctcaaaagct ggtggctcac gatgattacc
    24181 cggttttcaa aatccaaatc tttgtccgtc agtccgcaca gttccgaaat gcgaagccct
    24241 gtccccagca gaataacgat tgcgtcataa tgcttttggt aaaccttatc accttgaata
    24301 aaggacagaa aactttcttc ctgtgctggt gtaagaggca cctttggttc cgtatcgtcg
    24361 tccagaacgt cactaagctg gaagtcaaac gggttttttc ggatgcaatc gtcctgtatg
    24421 gcggtgtaaa aagccgcttt cagggaacgc ttgtcgttat tgatggtctt ataagaaagg
    24481 cccttttctt tcatccgaag cgcccattcc tttgcgtcgg acagcttcac actgtcaatc
    24541 gggcagctcc ccagccgatc ttgttccaga atacgcatca gccgttcccg gcccagtttg
    24601 gtgttgtgct tcacattgcc ccggtgcctg atctgcttgg cgtagagctg gcagacggtc
    24661 attttcttgc cgatggtgtc tatcccgtcg tcaaggtctt tctggatttc tttctccttt
    24721 tcccggagtg aaatatcctc ccgtttacct gccggggtct tatccgtagg cactaacttc
    24781 catgagtaaa caaactgcgg ttttccaaag gtatcaacat atttgtaagc atatcgtccg
    24841 tcttttctct ggctctctcc agaccgcaaa acgcgatttt tgctgtctcg tcttttttct
    24901 gacatctcgt catgctcctt tccatgacgg aaagagccgc atatatgctt ataaattatt
    24961 ataacatatc tgcggccctt tttcactatt agattgtgtc caaagtgtca attattttct
    25021 caaactgctt gcgtttgatc tggatgcggt tgccattcat aatgacccag ccagaggccg
    25081 gattttcctc ggccagctta cgcaatttgt tttctccgat acggaaatat ttagacgctt
    25141 cctcaatcgt cagcgtgtat ttttcccaaa taggcacatc atggttcata atgcagcccc
    25201 ctttcacaaa ttcaaaggtt tgtgtgattt aggggcatag acggacggct gctggcacag
    25261 ctccacggga ctttcacccc cgcgtggttc tcacgcggcc ctacccattg cctgcgacgc
    25321 ttttaacgct cggactgtgg ctataaggga gtatcatagt cctgcctgta cgtcgtcgcc
    25381 cgcaagccgc cacgctcaca ttgcggcgtg gtgttcctcg ctccgctttt cccgtaggga
    25441 aaagtatcat ggcgcaccag ccgtccggct cggcacagac agaatgtgtc cgtatgccct
    25501 atgatgcgcc gccgttatct tgcgggcata tttatcaagg tgcgatcagg ccaccgggaa
    25561 aacaccgaaa cggggaaagg gtaagccgtt tcatgctgcc ggggccggtt cgtcagttcg
    25621 tgacgatagc gtggatgagc ttgatttcca gacggcgctt catggcctcg tccagacaca
    25681 catgcggata gccgttttcg tcatagagcg tccgggtaca cagcttgttc atgtaaccgc
    25741 tgtaatactg caaaacgcgc tctacggctc cggcatcccc agcgcgggcg gcctcgatca
    25801 ccgaaagggg caaaagctca atgcccttgt agttcgggtt cttcatccgc gtctaacctc
    25861 ccttcggcat caacgccttt agctgtttcc gcagctccga taaggtattt gtcctgtgcc
    25921 gctgaacggc actccgggac attcccacaa ggccgccgat ctcaccatct gccatatcca
    25981 gaacacagtg caggattaaa atactctgct ccatgctggg cagcgcggca aaggcttcgg
    26041 caacaagctc attgttgata tgtaggtcat agccatgcga cgaaaacgta aagctgtcgc
    26101 tggggtagtg atctactgta cagagcttgt ccaaatctgc ctgtgacaaa gagcttagtg
    26161 acacttcacg gtcacggcgg cgcttcattt cagccaagta gcttttggcc tcgttacgca
    26221 gtaccgtctt gcaaaaagca tcgaacatgc accgcgcttc atagtcgcgg gggatagcat
    26281 ccatcttctc accccctttc tgtgggggac ttaggtggtt ctctcccttt ccgctaacaa
    26341 gactttgaca aggccgccag ctacccgcta aatacctcct ttttgaaaat ttctcaaaaa
    26401 atttttttgg ggacagccgg gccgcaaaag acgacaaaaa tcgcccggca ggtcgcaaac
    26461 ctaccgggcg agaaaatgct ttgctatgct gctgatttac atggtatagt acatactcat
    26521 aaccggaata gccctgtgga ggggcaaggc tttttttgag tggtggaagt ggattagagc
    26581 atggcaatag caaaacagca tagcggcatc ctcctatggg ccgccacacc ttttgctgcc
    26641 agaaaagaac ggcggccttg atttctcaaa accgccgcca gcaaaataca aaatgggcgt
    26701 gggaaaggtc tgcccaacag cggttttttt cttttctgcc gctgggcaga ttgaatacta
    26761 taatttgcaa tgtattacaa aagatgcttt aaccactttc cagtaaaaga ttgtccatga
    26821 ccagcataga ttttatcaaa tccaaaatct gatacaaaac caatactttc attatgaaac
    26881 ggcaaaatta aaataggtat cggggtatgg ctcatcaatc aacttatagt gccaccattc
    26941 aaagcgataa gactgaaatc cgctgctttc catgatttta cgcaagcatc ttcgattttg
    27001 tgcctctgca tcccctatcc cttttgctgt atgatgcgac cgttcgtcca taaaatcgaa
    27061 gttacttccc attgaaacaa gttcccctgt atccaggtgg tagagcgtaa gatcaattgc
    27121 gcttccacgg ctatggctgg attgtgaggc cacataaccc tttgtaatca actcggctcg
    27181 ctctatattg gggtaaaatt tttcttttgt gagattgtcc tccggttgcg ctgcccaacg
    27241 caggaagcag tccaccgcag ttcttggccg atatccatcc cataaaagca agccatagcc
    27301 aagcgcagcc gcatggattt gtgcctcgcg cagagcaaac gccacggcct ttgtgccgat
    27361 gatgcgattc acctcatacc catccaccgg ttttcctgtg aagttatccc atgtggcgta
    27421 cttggcatcc caacggacgc catgcaacat ttcatctaaa aacaaaaaac cattttccat
    27481 acttatcacc tctttaacgc caatgtaatc aggctgtcaa tcagtgcagg aagcgtgatt
    27541 cctgcggcgg ccatcatacg tgggtagcgg ctgtacgatg taaaaccggg cagggtattg
    27601 acctcattta gaacgatgcc gccatcctcc tgcaaaaaaa gatcaacacg ggcaagccct
    27661 ctgcatccaa gcacccgata tactttcttt gccgtttcct gcacccgatt tcgttcctcg
    27721 accggaatgt ctgcgggaac tgtaatcatc gcattttctg agcctttttc cggctcgttt
    27781 tcctgatgga tgcggaaggt accgtggctc agccggattt gatccacttc gccgacaatc
    27841 aaatcatcct cgttccccat gaccgcacac ccgacctcac agcccgaaat cgcttgctca
    27901 attaagattt ttccatcata ttgtcctgcc gcttctatcg cagcgttaag ttcttccgta
    27961 ccgtttactt tggttacgcc aaaggacgaa cctgaccgtg ccggcttcac aaagacaggg
    28021 taggtaagcg cacccgcctc cggcttgtca cctttatcaa tcatttgaaa ttcgggaacg
    28081 gcgatgcccg cattttttgt aagaatgtag gccagtgatt tgtccatgca agctgcggag
    28141 ctttgaatat cacagcccac ataggggata ccagacaata caaacagccc ctgtatcgca
    28201 ccatcctccc cgcatttgcc atgcaaaacc gggaaagcca catcaatacg ccgtgtttcg
    28261 tattcgcttt ctttcatgac aagcagccca tgcgttttcc tatccgggga gagtatggcg
    28321 gggagactgt cggcttccca ttccgtacat ggcttcttgc atagcttcca tacaccgttt
    28381 tttgtaattc cgatgtagtg cggatcgaat ttttccgtat caatgttcgc agcaatttct
    28441 attgcggatt ttaccgacac atcatgttcc tccgagcaac cgccgaagat gattgcgact
    28501 tttattctat tcatgctgta aactcctttc aaaattgaga caattcctga ttgttttttc
    28561 tgtagtatcc tgcagcacac gctcagtgta gtacgccgta tggggtgtga tgatcacatt
    28621 cggcatcctt aaaagctgcg acaaaaaggg atggtcaagc actttctgcg agcaatcggt
    28681 ataaacaaac tgatcctcgc cctccaacac atccagtgcc gcaccaccca gctttccgct
    28741 ttccaacgcc tccaccagcg ccccggtatc gacaagcgct ccgcgcccgg tgttgattaa
    28801 aaatgcgcct tgcttcatct ctccaatttg cttctgaccg atcagatggc gggtatccgc
    28861 acaaagcggc acatggagcg taacaatatc gctgtttttt agaagctcat caagctggac
    28921 ataatctgca tccatttttc ggctgttatc ataggccagc acatggcatc caaatccccg
    28981 cagccttttg acgaccgctt gcccgatatg gccggttcca ataactccca cagtcatatc
    29041 ccccagttct ttcccccgga tacgatccaa tctgaaatct tgttgcgcca cggcgtgcat
    29101 ggtgggtttt gcaccccgta tggccatcag catcagcatc aaagcataat ccgcaacgct
    29161 gtccggcgaa tacgcaaccg tgccaaccga gattcccatt ctctcggcgg cagtcgtatc
    29221 aatgtgatcg cagccgatgc tgcgggtaga aatgtatttt accccgacct ttctcagcgc
    29281 aagaattgtc gcctcggaaa cctcggactt atggcctacg ctaacgcatt gattgccagc
    29341 ggccaatttt gcgttgtctg ccgatatcgc atcgctaatc agcgtaggga taatatgaaa
    29401 atccggcgat aaagcgcgga aagcatttgc ctcatcctgt tcgcatccaa aaacagtaat
    29461 gcccacactt tttctcattg gttctcctcc tgaatatcca cactttccgg gaggggataa
    29521 cagattttgc atttgtttgt ataaagcagc ttgcaatcta ttatttcccc ggtacctaag
    29581 gcaacttgca tccgtttaac ctgggcatat tcataaatcc cgccactttc acggacatac
    29641 tggatactgc catttgtaat tttgtataat gcttggggct ctttgtcggc ggacacctga
    29701 ccgatgattt tctcatcatc taaggtcacc catatttggt aggtgcagtc ggtatcgttt
    29761 cgcactttta aatcaatcca gccctctgaa atggttgcat ccaccccttt aatctcgtca
    29821 ctgtttggct ctggaaactc ctttacttcg tgacctctgc gctggataat tgtcaatggc
    29881 gtatgcagga acatccaaaa tagtaaattg ctcatctggc acataccgcc gcccgacatg
    29941 gtggtgagtt taccattggt caccgtaagg ccgtctttat agggggtatc tttgtccgca
    30001 tggcgtacca gccaccagaa agaaaagggt tcccccggtc ttatcaataa gccgtttaag
    30061 gtctttgctg ccagctttaa gttgaacacc ttgttttcct ggtatatcat gtcaaatccg
    30121 gtattgtggt tgtataatgc gcaatccgtt tcaaagagca aatgggaaag cgatttttcc
    30181 cctatcgtct gcgcatagcg acagccgtca aatctcattc ccgcataaaa gcacattttt
    30241 ctctgcgccc gtctcattgg aagcaggaac gggaaacgct gtgtcaatct ttttctgtcc
    30301 ataacataac atccttttct catttttctg gttttaaata ttcctcaagg caaacccctt
    30361 ggttgtacat ctctgtggca gcttcgatgc caacatatcg gtaatgccac ggctcgttgc
    30421 tcacaccggt tatctctgtc ttgtctggtg ggtagcgacg aataaaacca aaacgatagc
    30481 tgttttcatc cagccatcta taaacctcgt tgcctgttga atgaattccg tccgcattga
    30541 tatccacagc aagaccaagc tgatgctcgc tcgttcccgg cacggccacc caagtttctg
    30601 cttccgcttt agcctgtgca gaggtgtagc ctttcgcctt atattcggca attttttcat
    30661 ccataatttc ttgctgtttt tctgttgttc ggtatccgga tgcgacaatc gggtaaactc
    30721 catcagttct tgcggcatca aacaaatctt gaagataggg agaaatccga atatctatcc
    30781 gctcaccatt tgatagttgc tcaagctcca catcgtactg tgcggggatg gggttctgcc
    30841 tgttcactaa aatcaggctc cattcctgtt catctccctg ttctttggcg gtggcaagag
    30901 cgttttcctc caaattctcc actacgtcag tgctgctgcc tgtatgagtt gcagggatgg
    30961 gcggtatctg tgttgcctcc aatacggaag ccagtctccg ccccaaaaac agcgtgcaga
    31021 ctgtgaacga aataatgaac gcacatagaa aagcccgctt ttccacggat tgtttcatat
    31081 gccgtttgtg atgatccgca ttggaatgat agttgctttt ttccatttga tcgaatgcct
    31141 cctttgcaat aaaactacga tttgtggctg gtacgattgt tattatggca agcgcatctg
    31201 tactttccct gtagccagtc tgtagttttc ctgtagcagc aaaaaaagac cggcagggtt
    31261 atacctgtcg gtcaaaatca tttaagtttt aaatatttac aatgttgatg tgagcggtaa
    31321 atccagccag aacaaaacgc catccgaggt gttttccagc gcatactgga ggctcatggc
    31381 atccagcgtt ttttgtacga tggcaagtcc taaaccgctt cgcccacttt ttctgctttg
    31441 cgcctgatca atgcgataga atggggtgaa cagccttgga agcgcagtat catcaatgtg
    31501 aacgcccatg ttcaaaacaa aaaggcggca tttttcagca ccaggctcac tccatatccg
    31561 tacctcgcct ccctgcggcg tgttctgaac tgcattcaat atgacattgg atagtgcctt
    31621 ttggagcagc ctcggatcgg acaggacaat ttgcccggct ggaatatctg tgacgaaccg
    31681 ctggttgttt gcctctgcca aggtttgaaa atcgggcagc aactcggcaa ccgtgcgccc
    31741 tatgtccaac ggttcagcta tgggtacgat tctcccatca ttcaggctga caagctccag
    31801 tatttcggaa atgattttgc cctgcctatc catcattttg atgcattcgc gcagatactt
    31861 agaatgatct ttgtagtcac cgatattttc aagcattccc tccaacagaa cgcttgtagc
    31921 cgcgatgggc gtttttagct catgagaagc tgccgcaaag aaatatcgct gtgtttcctc
    31981 caactcatgt tcccttgcga tttcatcctc cagccttgcg atggtttctt tcagcctgac
    32041 atacatggaa tgcatatcat gagccagtgc gccaagctca tcctttcgct ccagcggcgg
    32101 cggtacatct ttcaggtttg ccattttatt cgcactgtcc gctaaggctt tgatcggcgt
    32161 tgtcatttgc cgcgcaaaga tatacgcgca taaaaggctg aatacaacca ttatcgcaat
    32221 cataacaatt ccccgaattg tcagcccttg ataaagcaat cccacacctg ccttgctttg
    32281 agcaacaatc gaaatattat catctctatg taccacataa agaaagtcgg gtctaaaact
    32341 atttgatgta ttggcatttg gtgtggcata gagtacgctt ccctctttat cttcaatata
    32401 aaactcaaag gattggttat tgtagtgaaa cagccctgcc acctcttgaa tatcaagcct
    32461 atcgctattc tgaatcagtt ccaccaatgg ctgataggat tttactgttt gctgtaattc
    32521 catcactctg aaataagaca caaattgctg tgcaaacagt gttgctgtta caccgacaag
    32581 cagtagcagg gcaatgatcg tataggaaaa aaccttaatg aaaatccctt ttctttccat
    32641 tattatgatt cctccaatcg gtatcctaca ccgcgaatgg ttttgatgat attttccggc
    32701 agcttggccc gcaaattttt gatatgagtg tggactgtgc tgccatcacc ctcaaagtca
    32761 tatccccata tacgtgacaa aatgatttca tgcgttaagg ttctgccttt gttctgcacc
    32821 agcagcaaaa ggatttcaaa ttcttttcgt gtcaggggca gctctgtacc gtcacaaagt
    32881 accgtgaaat cctccggcag aagtgtcagc ctgccgacac ggatttcctt tgccagcgca
    32941 ccgctgcgcc gcagcagggc ttccacccgc tttagtaaaa tctgcatctt gaatggcttt
    33001 gttacatagt cgtctgcctc tgcatcaaac gcccggattt ggttttcgtc atccgacagg
    33061 gctgtcatca tcagaatggg agtatcattt ttcgcacgaa attcacgcaa aagttcatgc
    33121 ccgttcatac cgggcagcat aatatcaaga ataaccagtt gataagtgtt ttcgtaaaac
    33181 ttggtgtatg cctcatttcc atctgtgcag gcatccacct gatatcctgc ctcagccaga
    33241 aacgccctta ctgtattaca gatatgatca tcatcctcga caagtagaat tcgtatcgac
    33301 ataaaaatcc ctccatttta agaagataac ataacagtct gtagtttccc tgtagtttca
    33361 aagcgatttc ttttcggatt ttaatcatcc gtcattggga tactcgcttc ttttcatggt
    33421 gtatgtatct atattattgg tttaaacgaa ttaaatcaag ataataataa ttaacacctt
    33481 cttaatgttt gggatgtttc gattacagcg gttgtcaaaa catcccgata atactggcaa
    33541 gcaatggatc agaatatcct ttacggatat tctgatccac cacttgcccg cctgcggcgt
    33601 tctcgttttg cttgttgggg acaccccaag cctcagcaaa atgcgcggcc tcttttttgc
    33661 gaatgcacct tgcggtatgg cttttcacac aggcagggcc tgtgtgtact ccctccggtc
    33721 gctcttgccg cactccgcgc agcagattca gtcctaatac cgttcaagac agtagaaaat
    33781 cagcaagtct atattcctca tatcttcatc atccgtgtcc ttgcagttac aggaaatgta
    33841 ataatcgcca aatgtaaatc ccagaatatt ttcctcaaaa agccgtacct ccccggtatt
    33901 aaaatcaatc accccatact caaaggcgca ttagaccagc agttatccaa tgtctggaga
    33961 cttcaccata aagaatccag atgccggcct tttcaattct accatatacg gctgatacag
    34021 cagcagcttc ctcgtctttg tttcgggcat tttccatttc tgtcatgata aagtttcgct
    34081 tcttcataca tccatgtgta aatcttttct ttctgtttca gatgagaata ctttttatcg
    34141 gtctggagca gcctgccatc tatcatctta tagtttttcc gtgacaaaat accatgcttt
    34201 ctctctcttc aaaaccatat ccaaaatgcg ccggtttcat tatactattc agtaaccgcc
    34261 tttcctttct ttgtacgcct gtcaatcggc ttttccgcag ttttgggaat gagccagatc
    34321 gtcgccatct ttacagcgcc ggggatgcgt cccgcagcac aatagtagtt tatccatcgt
    34381 gggggcacac cccatttctc gcctgcctct ttcaacgtca tgtagtccat agtacacctc
    34441 cacagggtac attataattc ttttgctcga agaataaaag aaacaaaatg tgaaatataa
    34501 actttaagaa aggtgcatat accaacattc cacgggcaga gtatgaaata tacaatgtaa
    34561 gtgggaggtg cgatatggcg aatattgaag attgtcccgg ctttgaaact ttcggcgcgg
    34621 atgtgaaagc ggcccggcaa gcaaagcgcg tttcgcgcaa agcaatggca gagaaaataa
    34681 atattgactg gcgctatctt gccaacatcg aaaacgacgg ggccattccc agtctgccgg
    34741 ttgtcataca gcttattaaa gcctgcgggc ttcctgtgga acgctacttt aacccggaga
    34801 tcatgcggga ggaaagcgaa ctgcggcaaa gggtcagcca caagctgaaa ctctgccccg
    34861 aagaatacct gccgatcatc gagggcgcga ttgacagcgc catactaatt gaaaagccga
    34921 agaagaatga aacggaggat gtgtaactgg catcctctgt tttactttta gcgggagctg
    34981 cacagcgtag cttctatttt tttgcacttt tttctttcca gagggctatt ttgcgggtag
    35041 tttggggctt tggaaacgtc ttgttagtgg agaaacagta acgtagcaag catagggagt
    35101 gtagccactc ccggaaaaac ggcctgcccc gtccctgccg ggccgcgtcc gttttgcttg
    35161 ttggggaaat ccccaaaccc ctgaatggaa aggacggtga agcatggcaa acagaaagcg
    35221 aaaaatcgtg ctgcgcgtcc ctgtgacgcc ggaggaacgg gctatgattg aacagaaaat
    35281 ggcgctgctg catacgaaga acttttcagc ctacgcccgg aagatgctca ttgacggcta
    35341 tatcgtcaac atggacacca gcgacatccg ggcgcagacc gccgagatac agaagatcgg
    35401 cgtcaacgtc aatcagattg caaagaggct gaacggtatg gggccggtgt acgcacagga
    35461 catcgaggat ataaaggggg cgctggcgca gatatggcag ttacaaagat acatcctatc
    35521 aagtcaacgc tgaaaaaagc ccttgactac atcgagaacc cggacaaaac tgacggcaag
    35581 ctgctggtgg cctcctttgc ctgctcttat gaaacagcgg acattgaatt tgaaatgctg
    35641 ctggcgcagg cataccaaaa gggcaataat ctgggccacc acctgataca atcctttgcc
    35701 cccggcgaag ccacgccgga acaggcccat gagatcggga agcagcttgc cgaggaagtg
    35761 ctgcaaggga aatatcccta tgtgctgact acccatattg ataagggcca cgtccacaat
    35821 cacattattt tctgtgccgt ggatatggta aatcagcgca agtacatttc caacaaacag
    35881 agctatgcct atatccggcg caccagcgac aggctgtgca aggaaaacgg attgtccgtg
    35941 gtgaagcccg gccagagtaa gggcaagagc tatgccgagt gggacgccca gcggaaaggg
    36001 acgagctgga aagcgaagct caaagcagcc attgacgccg ctatcccgca ggcaaaagat
    36061 tttgatgatt tcctgcggct catgcaggcg caaggctatg agatcaagcc gggaaagttt
    36121 atttctttcc gcgctcccgg acaggaacgc tttacccgct gcaagacatt gggggaagcc
    36181 tacaccgagg aagccatcaa agagcgtatc aaaggccgcg ttatcaccag agcgcccaaa
    36241 gagcgcaagg ggatttcttt gcggattgat ctggaaaaca acattaaggc ccagcagtcg
    36301 gcaggctatg agaggtgggc gaagctccac aatttgaagc aggccgcaaa gactttgaat
    36361 ttcctgactg aacatgggat agacacctac ccggatttgg aaagcaaagt ggccgagatc
    36421 accgccgcca gcgacgaggc cgccgctacc ctcaaagcta tggaacaccg gcttgcggat
    36481 atggcggtgc tgataaagaa tatttctacc tacaagcagc tctggcccgt ggctatggaa
    36541 taccgcaatg cagcggataa ggcaaaattc cggcgtgagc atgaaagcac gttgattttg
    36601 tatgaagctg cggcaaaggc cctaaaggga cagggtgtga aaaagctccc cgatctgtac
    36661 gccctgaaag ccgagtacaa gcgattggcc gaggaaaagg aacggctgta tgagaagtac
    36721 ggcgaagcga aaaagcagat gcaggaatac ggcatcatca agcagaatgt ggacggcatt
    36781 ttgcgtatga cgccgggaaa ggaatggacg caagagctat gagtattcca cgaatgaatt
    36841 accctcaata tcgcaaggcc cggaagctga cgcatgagtg ctgcaactat tgtgacggca
    36901 actgcctgtt gctggacgac ggcgaagaat gtatttgcgt acagagcatt tcctattcgc
    36961 ttctgtgccg ctggtttaag gccgctgtcc tcccgctgga tgcggccctg tatgccgaga
    37021 tcaccaaaga ccgggacgaa gtgaaacgct gctgtgagtg cggggccacg ttcacgccga
    37081 agtctaaccg ggccaaatac tgcgcggcct gctccaaacg gatgcgccgc cgcaaagagg
    37141 ccgagcgcca gcggaaaaga tactggaaac ctacgcactt agagctgtaa aagcccttat
    37201 atttcaaggc ttttccggca tggtcagcgg ggtatgtggg tgttttatcc tatccccgcc
    37261 gaagtgctgt tctaaatgcg tagaatttac cagcagaaaa gcgccgtttt ctctggttcc
    37321 atgcttccga atatggtatc atagaaaaaa tcagtctgac gggggtatca ggatggacgg
    37381 acaagaaaag ctgctggact atgaaacgat caaagccgcc gttgccggtg agaggtgggc
    37441 cacggaaaaa gtgttggccc attatgctga ctacatcaac gagctttcca cggtggagat
    37501 cagacaggca aacggcaaaa agaagaaagt cattgacgaa gatatacggc agcgcatttc
    37561 cctgaaactg ctggaagcat tgccgagctt cccgctggaa cagagataga cggcaagagg
    37621 gctgcaccct cttacgccgc tttgcggcta tcacctgcac acccctgaaa cagatacacc
    37681 cgctgcgcgg ctatatctgt ttcaggggtc taattatagc tgcaacctac gcatttaagg
    37741 gattgaaaac cgcccgctgc aaggcttctc ccgcatgttt gcaggggcaa gcgaatactt
    37801 catcctatcc ccgacaatac cgggttctaa atgcgtagga ataggcaaaa agaaaagacg
    37861 ccggacgcaa gcgaccacat ggccgccgcg cccggcgtct gctgttcgtt attcgtctgt
    37921 gaagtggaac ttctggccgt catagtcggc atccgtcatg cgttccagct tggaaagggt
    37981 ctgctgggcc tgcgcccgca tatccccatc cataaccagc atgacggcgg tgagcctgtt
    38041 tatcgttctg cgccggtctg cattgtggta catacagatc aggttttctt cctccacggt
    38101 aaagtgcatc attccttata cctccaattc attctttttg gttctggccg ccgccttttt
    38161 tgggctggcg gcctgtttgc tgtcggatag ctgcttgcgg atagagggcc ggggcctgcg
    38221 aatactcaca tcccggcgct cgtcccggtt aatcacggca tacacgccat gcctgccttt
    38281 catcccggta aaacacaggg atttataggg cagcatggca aagagccggt cattatcctt
    38341 ggtggaagca atcgccgcaa agtgcgggga gatctccacc atgaaatgcg ttttattggg
    38401 gctgttaggc gcggacagat ctttcaggct gtccacaata cgccgggcct cggtttcaat
    38461 ccggctgtca tgcaaagcgg tgacgtggac tttgaaatcg ccggggacgg cccgcccccg
    38521 gctgtgctgc tccgtccgta gcgcctcctg cagcacagcc gggtcatggg gctgggcctc
    38581 atagtacaaa aagtttccca atacccggtc agggcttccg tcaaaacgct ggctctccgg
    38641 gatgtcacgg gtgccatgct cataaaagag ccggttcaca ctcaccggct gggcggtttc
    38701 aatgacgtgc cggaaatgct gcggatagtc cagctcatag agattgcccc ggatagtgcc
    38761 gccctgtgtg ccggtcagct ccaccgcgta ggccagtatc ttgtcacggg tctgctcccc
    38821 gtaaaatttc catgtgttgt actgtcgggt gtctttcagg aaaacatcac gttcccggaa
    38881 acagtacgtc ccggagggcc gggacatcca gagcagggtc ttgtctgcgg cctgatcgct
    38941 ggcggcatcc cgcctgataa tgtctttgtc aatgccaaag tcactttgat aaaacgcggt
    39001 gttctgccgc atgatctgtt ccagcgcggc cagaacgtcc acattttcaa acttctgcat
    39061 ttactctacc tccaatccct ttgatttttc ccgcgccggt ggctgcttgg cctgttcggt
    39121 cttggccgcg ttgagctgcg cccggataga gggcttttct ttctccctgc gggccgcaag
    39181 ctgtaaggcg ctgggctttt cgccggaaag gggctggatg cacttgatac ggtcagcgac
    39241 gcggattgtg gtgtcgttta actgccgctg ccatgccccg gccttgggcg accagcggaa
    39301 accgttgctt ttcagctcgt cgcgtacagc agcatccggc ttcccctcaa agaagatttg
    39361 caggcggttg gccacccggt tgatctccac agtgccgccg tcaaactccc atcccacata
    39421 ggccgcctcc ttttggcggg tcaattcctc aatgcggccc tttaaccggc gtatctcggc
    39481 gttattgttg gagagctgcc aggactggaa cggttttttc tcatagtgcc agcccgacgc
    39541 catatccgct ttcaggtttt caatgcctct ttcggtcaga tgggggcatc cgtccagcgt
    39601 cccatgcttg cggtagtagg cattgacggc cttcatcaac tcctgcgacg cttccagctt
    39661 ttccagtttc ttttccagct tggaaacggc ctgcggatcg tcggcgctga taccacccat
    39721 gccggtgctg cggattttgt caagcaagcc ctgaatgtac tgaaattcct ccatgttgcg
    39781 gtcactggcg gcgttctgct tttctttcgc ccgtaccggg aaattggacg gcccggcaat
    39841 caggacagag gggacgcggg cggtaatctc atagccctta ttcatgttct cggccagctt
    39901 gcgggcgtag gtgtcaagga ggctgtctat cttttcgtga tactctggct ccacacgctt
    39961 tttctggcgc tccgcaattt ccacggcctg atcgacatag tgccggtatt ccgccgtggc
    40021 gctgccgggc cggtagtcag aaaagctcat ggcttccttg gcgcggcggg cggctccctc
    40081 attgatggga taatagcgga cagccgtttg cgggggcgca gcctccgggg gttcctcctg
    40141 tgtctggccg gaagcaactg ccacatcctc aaaagatagc tgttcgggtt cctccggctg
    40201 cggttcgggc ggcgtattgt tgataatgcc gtcaatcatg ttttcgttct gctccgtaga
    40261 cagttcggcg gtacgcaggg gattttcccg caaaaactcc ggcacttcca tatagccgaa
    40321 gctgtctaca tagtgggcgg tgtcctgtcc attctggtgc agcaccacca cgtcggaaac
    40381 ggaaaggctg tgtcccctaa aatctgccgg gtggtcgatg ttgaatgtgc ggtaaatatc
    40441 ctccaaagac gtgcttttat ccagcggtgc cgtataaaca aggtcatagt tggcactgtc
    40501 cacggccagc cctgcggcct gtaaacgctc gtatggctca aaccgatagt cgcgggtttc
    40561 ctccccgcct cgaagctggt agatggaaaa ggtgtcctgt tggggtgctt catagccagc
    40621 cgccgcgtat tcctccacgg tcataccggc tgcggcggcc tcctgcgcga tctcggcctg
    40681 tacctgtgct ttgtagtcct gcaagctctg gtcgaagtca gccagtttcg gcggctccgg
    40741 caactgatac tggccctgat tgagcatggg gcggcactcc tgcacataag agagaagcgc
    40801 ccccaaatag gccgtttcct ccggcgtcag ggtttccccg gactgtaagc ggccctgcgc
    40861 cgtctgttcc atttgggcaa ggctgcaatg cagcttcata taggggacaa actcgcctaa
    40921 cagcatatcc cgatgggcct tgtcctgctc ccagccctct atgccggttg tagcgattac
    40981 atgctttttc cagttttcgt cctgcgcgta aaattggtgg tagctctgga tatgttcgat
    41041 cagggaaccg tccctatctc caaaatcctg ccgcccctcg tagctgtccg gctcgccctg
    41101 aaagatacag tcaatgcgga acgcggtttt gtcatagcct gcctctgccg gatatgctgc
    41161 atccagacgg gcaaacagcg cgtcggcctc atgcaacggc atttgctggc cgtcctttag
    41221 ctgggggctt tcgctccata gcacagtcac aagcggctcg gctgccggat caagggccgg
    41281 ggcctgttct tcctcggcct gcgccggggc cgctgcctcc ggggtaacgt caaccgtgac
    41341 ggcctcctgt tcctgccggg cctcgcgctc ctgctgaagc tgggcaaaat ggccgtcgat
    41401 ctcggttatc aattcgctgg ccgtggcccg gatggtttca agggaggctt tcagctcgga
    41461 taactccctg ccgctgctcc aatcggccac atagccaaaa gaatagtccg acgtgtccag
    41521 cccgtaatac tggcagacgg tataggccac gctttcggcc tgtacctcgc gggtgctgcg
    41581 gtctggccgg gccgcctgtt cctcggcggg ggcctcccgg tcaatggcgt ggagcttggc
    41641 atgggcaatc tcatggatgg cggtctttag ggtctgcaat tcgctcatgc cctcgttgat
    41701 ggcaatgcgc tgttttgcgt agaagcagcg gcccttggcc ccgtctgaaa tcggctcaat
    41761 gcttatggga tagggtgatg tccgttccag cgcggcaaag aaatcctgat actgctgcac
    41821 gtcgccggta agttcactga tatagggcat gggaaaatcc ttgccctcgg tctggctgat
    41881 gtcaaagact gtcaccacct taaaggccgg aaccgtcact tcctgttctt cggtcacggg
    41941 cttgccgtcc gcgccgatca cgggctgctg ggtggccggg tcgattttct ccatctgctg
    42001 cttaaccttg aacggggccg gggccagtat cttaatgcct ttctcgcccc gcttcacatg
    42061 gcggtcaaat tcatcctgcc acctgttata gcccgccaca atcgaaccgc cctgcatggc
    42121 gatcagcagc gtattgttca gactgtaatc gtgcatcttg gacatagcgg ataagtaagc
    42181 catatagcgg ctactttcaa aaagctcctg tacgccggtt tccagacggt cagtaatcac
    42241 tttcatgcgt tctttgctgt tcatgccgtc cagcatgatc gggataaccg ggggcggcgc
    42301 tttgtcgggg ctttcggatg cctccggctg ctgggcctgc ggttcctccc ggtcagcttc
    42361 cggctggggg tagtgcatca cccggtattc ctccggcacc ggctggccct catggacgcg
    42421 ctgccattcg tcgccgcttc tcaccagata gccgtactgg gtaaaaacgc cttgttcctg
    42481 ctgggcaatg tggcgtccaa actgcgccgt atcaatcccg gctttccatt cctccggcat
    42541 atccaccatg ccggaacggt tcaggtagta gtcgcccaac tgtccatccc ccctaacgtc
    42601 gggaagatgc acatagtaat ccacattttc ggtgtagtcg atgatctggc cgatattctc
    42661 aaagccgcca cggggatttt gcagggcggc gttcagctct gcgatctcgg cggcgtcaag
    42721 ctggcccagc cgggcggcaa ggaaattcag ctcgtccacg tcggcggcca gcaccatgtc
    42781 atagggaatg gcaaggtgtc tgtcctccgg gcaggaatac ccgttgataa aaaaatcctg
    42841 cggattgtcg gcagtaatgc caacatcccg cagggcggct tgcagcgttt cggttgtggt
    42901 gggcaaatcc agccagacgc cttgtttgcc ctgttccaaa aactgccggt tatggacttg
    42961 gatagaaaat acttcgttca cgcggtcact cctttctctg ttctgtttca aaaaataggg
    43021 ggttggggac ttccccaaca agcaaaaatc cccggcccgg cagggacgcg gccgggggat
    43081 tttaccggga gtgtagccac actccctatg cttgctgtgt tactgtcctt tagctcctgt
    43141 gttcaatgcg gtagttgacg tacttgcccc cggtatcagc caaaagcacc gtcccgtcgt
    43201 aggttttgcc ggtctttacg gaatagaggc cctttacctt gactttgccg gttttgagca
    43261 gcgcagcggc gatcttcggg gtgaacgctt tcttgcgttc ctcaaaaaat cggtcattct
    43321 tccacatgac gaactggcag gcccggtttc cgcagtagta gtttttcttg ccctcataga
    43381 cagcctcacc acagcgggga cacgtcccga taggaacacg ctcggcctga aacaatttct
    43441 gcttttcctc actgatacag gaataggtct gcaccagctc ccgcgcctgc gtctcgatgc
    43501 cgtccataaa ttcgtcagga tcggcctgcc ccttggcgat ctcggtcagc cggttttccc
    43561 attcggcagt aagctgggga gaagtaagcg cctccggcag caccacagcc agattgatgc
    43621 cgtccttggt gggaatgagc tgcttgcctt ttctctggac aaagcccatc tgcaccagct
    43681 tttccagaat ggcggcgcgg gtggccggtg tgcctaatcc cttgcgctcg gcatcctccg
    43741 gcatatcctc ggccccggcc cgttccatag cggacaaaag cgtatcttcg gtgtagggct
    43801 tgggcggcgt ggtgaaatgc tcggtgacag aggcggccac gtctgcaaag gtctggccct
    43861 cgcaaagctc cggcagctcc cgcaccgcct cggcgtcctc gtcggcgtcg gtctttaagg
    43921 aagaacggaa ccggcgttca atctctttcc agccggggga aaggacggtc ttgcccttgg
    43981 cggtaaactc ctgcccggca caggtaaatg tggcggtgac ggcctcatag atatgcggcc
    44041 cggccacggc acataagagc ttgcagcaga tcagggacag cagctttttt tcactctcgg
    44101 ccagagtgcc aaagccctgc ttcacaaatt cagcggtggg gataatggcg tggtggtcgg
    44161 acaccttttt gctgttcagg atgcggtcaa actgcgggga aaggttcagg cccttggcaa
    44221 acggcaccag ctcccacagg ccggacacaa ggctttccac ggcgggcagc atatcgtcgg
    44281 taagatattg actatccgtt ctcggatagg tcagcagctt ctttagatac agtgcctggg
    44341 catagtcaag ggtctgcttg gcggtatagc cgaaaagccg gttagcctcc cgctgcaagg
    44401 tggtaaggtc atagagcttg ggcggctgtt ccgtcttttt ctcccgcgtc acggaaacgc
    44461 aaacggcctg cgcttttgca caagccgcct tgacggtgtt tgcctcggca gcgtcggaaa
    44521 agcggctgct taccgcgtcc gcgccgcccg ccgtgatatg gacgatgtga tatttctctt
    44581 tcttgaaatc ggtaatcttg gcgtccctgt ccaccagcat ttttaaggtc ggcgtctgga
    44641 cgcggcccac agtcaggttc ttgtggtaca ggacggaaaa aagccgggtg gcgttaatgc
    44701 cgatcagcca gtcggccttg gcccggcaca gggccgattg atacagcgga tcatagtcgc
    44761 cgcccggttt caggtgggca aagccgtcct taatagcact gtcctccata gaggaaatcc
    44821 aaaggcgctc aaagggcttt tggcatcccg ccatctggta gacgaaacgg aaaatcaatt
    44881 ccccctcgcg cccggcgtcg gtggcgcaca caaggccggt cacgtcgggg cgctgcatca
    44941 gcgaacagag aacgtcaaac tgctgtttct tgtcgtcggg gacgatgtac tgccatgttt
    45001 ccgggagaat gggcaaatcc tcatagcgcc attttttgaa acgctcgtca tagcgtccgg
    45061 cgtcggcaaa ggacaccaga tgtccgatgc accagctcac cagatagccg ttcccctcca
    45121 aatagccgtc gtgtttctcc gtggccccga tcacgcccgc aatgctgcgg gccacgctgg
    45181 gtttttccgc gattacaagt ttatatgtca ttcctcgtcc tcctgttcac tcacaaaatc
    45241 agcttccggg tcaaactcgg tttcgggttc ctcgtcctcc aaatcctcgt cctccccaaa
    45301 atcatactcg cccaaatcgt cgctgccggt ggtttcagcc ttgggctttt tgaacttgaa
    45361 atagtaaagg gccgcaccgc cgcccgccag cgccaccagc aggaacacga tcaggccgcc
    45421 cgcgccgccc ttgggttctt cctccggggc ttcgggttcc tccggctggg gttcctgcgg
    45481 ctccgggccg ctgcactcgg tcaggttggt tttacatacc gggcaattcg tgttcacagc
    45541 cccggccttg catttcgtgg tacaggagca gacagcagcg ggggtgacgg tttccccgtc
    45601 ctccatcagc gtcagcagat cggcttcatc cacctgattt aagaaatgga cagtttcttt
    45661 tccctcgtcg tcccggtcaa taatcagata aaacacattg ccgtttttgg tttccacggt
    45721 gataaactgc ttgccatttt tcgcggcgct gccgatgtcg tcaatcagtg acaggttccc
    45781 gtccggggac agggcggcgc tggccccgcc gccattgaac agttccagca gagaagcaat
    45841 ttcctccggg tcaatggaga gggaaaaacc gccctcgccg gtagccgcta cttccttttc
    45901 gctgacggtc atatagtcgg aatggacata gccgatgcgc tccggcaaga gaattttaat
    45961 ccagtcgccg tctgtgccga ctacctcaac cgtcgtccca ttgggaagct gggtgaacgc
    46021 ggtcttgtca agccccgcgc cggtgcggac attcagcctg ccgccgttgg tggtgacggt
    46081 gccggtctgt tcaggttctt ttttgtcggt gccggtggtg atctggatgc cgtcgtccgt
    46141 ggcggcaatg ctcaccttgg aaccgaacag cgcggaaaac aggtcggtga tgtccttgcc
    46201 ggtcagaggg ccggtatctt tttcggcctc ggtttctgtc ccgtccgtgg cggtgccggt
    46261 ttcgtctgtt tcaggctcca aaccgcccgt agcgggttcg gttgtagcct ccggcagcgg
    46321 ggccggtgcc gggtcattgc cgcctgcaag ggcggtaacg ggaaaggccg tgacgcagag
    46381 catcacggcc agaagtgcgg ggagaatacg ggaaatccgt ctggtcttat tcttcatggg
    46441 cattgtcctc ctgttcatgg taaggggttg cggcaggggc ggcctgcatt gcgccgctgc
    46501 gtaagaacgc ggcaaattcc tccggcttca tgttcatgcc gcgcacaagc tggatgatct
    46561 gcaaattctc ctgttcggtt ttctgcgttt ccagctcccg aagctgtttc tgaaactccg
    46621 cgatcttaga cttggttttc tggatgtcct tttcgatgcg gtcaagtttg gttgccataa
    46681 ttgaaagctc ctttctggcg ttaaggtaaa cgcccgtaac tgtaaaaatg ggattgccag
    46741 tagtttgaag taaggttcgt gtaggaaatc gggttcccgc aatgcagcat cacattgttg
    46801 cccacataca ggccgacgtg ggacacgccg ggggtgtcgt aggttccctt gaaaaataca
    46861 aggtcgccgg gcttggcctg cgaactggaa acaggggtgc agatgttata taggccctgc
    46921 gcccccagcc gtcccacgtt ccatccacta tgattgatta cccacgaaat gaagccgctg
    46981 caatcaaagc tggtgctggg agagctgccg ccccacacat agggataacc gatatatttt
    47041 tccgcctctt tgagcatggc ggcaaaggtg gcatcctcca aagcctccgg gggtacgtcg
    47101 tagtaggtcg gcggcgtcag ggtggaggcg ttggggtact ggctgccgga aaacagatca
    47161 ggccggtttc ctagcgtgga catatacagg gaatagaaac ttagcgtttc ctcgtccatg
    47221 atatagactg gcacatggga caggtcgaag ttttccagcg tgacgtagca gatgtaatag
    47281 tcatacggca cttggtaggt gtctgtatgg gtgttgccct ccgcgtccgt ccatgtatcg
    47341 gtttcggtgc ggtaacgggt ttctaccacg acgttttctg tcaggatata ttgcttgtca
    47401 aagagcatgg acaaatcacc ctgtacctcc ccgatggtaa atatcccgtc atggagcgcc
    47461 gacagaatgg aaatcagcac ataggggtca tggctgatct cgtcaaggtc aaaatggtat
    47521 tcgtcatagt catgggtggc ctcgtagttg tccagatagt cttgcagctc ggcttccagc
    47581 tccgcgtagg cgtcctcggc ggccaacata tcgctatcct cggaaaagta agaggacgcc
    47641 gtgatgccgc ttccggcgct gccgaacatg gcggtgcagg actgtaagcc gccgatgata
    47701 agcagcatca aaagccccaa cacaaggacg gccagcgccc ctttccagtg gcgggccaca
    47761 aaggaggccg cccgttcact ttctttggct gcggccttgg ccgctttcgt ggaggccccg
    47821 gctgtcttgg ccgcgccctg tgccgccgcg cctgcggtct ggcccgctgt ccgggcggcc
    47881 ttggcgtact ggcgcttgat ctgctgtttc tgccagaacc gggaaatggg gttgctggcc
    47941 gcctgtgcga tctccgggtt atcccgcaaa gatttctggt agaaatactc cgcgttggca
    48001 gacaatagcc tgcgctcggc cttttccgcg tcccagtagg gtttcagcct gtggtgccgg
    48061 atggtgctgc ggatggcttt tccggcctgc cgctcggcaa gttcctcccc cttatggccg
    48121 ccctccacac ccacgttttc atgctcgact tcatggattt tcccgtggcc gaatagaaaa
    48181 acctcggaaa caggacggcc tgccggattg ggtttcaggg agggaggccc cttgtcctgt
    48241 ttgtcaaagt gcaggacggt ctttgctttg ccgcttgcct cgtcaaagac ggtttccttg
    48301 accgggacgc gcttcttggg gatggcggcg cgggccttat ccagcttatc ggcccgtttg
    48361 tccgattttc ggatataggg ctgtaaatcc gggtcggccc gttcttcctc cgacagttgg
    48421 agccgggcgg atggccggtg cagggcagcg tccccctcgg cggccacagc cccggcctgc
    48481 ttcgcggctt tcttggcctt atggccgtca tggatggcgt cggcccgttc cagcagctta
    48541 tcggcggtgc cgcccggccc cggcgcggac aaatcctgtt cggcctcccg gctggaaatg
    48601 ttctccacct cgccggtggc ctgattttcc agcgtcagcc cgtcgcgggt catggcctgc
    48661 gtcactttcg ccttggcttt cagctctttc atggtggttc acctcctttg tggcgtcgcc
    48721 gggtcatact cccgccacct cgtttaagcg ggtcgtcata atcttgtaaa gctgggtgtt
    48781 gtgcgggatg gggttggtaa agggcagcac cacgttttca aagatcaaaa ggccctcgcc
    48841 gggttcggaa ttgtcgatgt acttctgctg ttcggtggac aaattcaagc gttccgcaag
    48901 gatttttcta tccccggacg cctgattgag cagcgtaatg aagtcgctgt tttcaaggat
    48961 attttcaatt tccggggagg aaagcaggtc tttgacgttc tgggtggcac ctgttggaat
    49021 gccgccccat ttcctaaacc gcttccaaat ctccgcagaa taggccgccg tctgttcctc
    49081 tttcaaaagc aaatggaact cgtcggcaaa ataccatgtg gcctttcctt ggcttcggtt
    49141 ggccgtgacg cgcccccata tctggtcttg gacaatgagc atccccagct ttttgagctg
    49201 cttgcccagc tccttaatgt caaaggacac aagccggttg ccgatgtcca cgttggtacg
    49261 gtggttaaat acgttcaggg agccggacac atacaggtca agggcctgcg ccacccggtc
    49321 agcctccggg acatgctgat ctaacaacgc tttgtggagg tcggacagga tcggcatatt
    49381 ctccgggcgg gggtctgcca gatagggccg gtagatcacg cgcacagccc ggtctatcac
    49441 ggtcttttca atggcttcca gcccgttctt gccgcccatg acaagctcac agaacgacag
    49501 cacaaagtcg gatttcaggg ccagcgggtt atcgtcctcc gaataattca ggttgatgtc
    49561 cagcgggttc acaaagtcct tgctggtagg cgacagccgg attacctgtc cgtggagccg
    49621 tttgacaagg gggtaatatt cggcctccgg gtctgaaata aaaatatcgt caggcgtcgt
    49681 taagaacaca ctgacgatct cggatttaca gctcatggat ttgccgctgc cgggggtgcc
    49741 gagcttcaag gcattggggc aacgggcctg cttcctgtcc agcataatca tgttgcggga
    49801 cttggcgttg atgccgtaat acatggccgc gccgccctga aaaagctcct gcgtcacaaa
    49861 ggggacgaac acggccacgc tggaagtcgt aaggctgcgc tgtatcttga tctgattgac
    49921 gcccagcggc aggctgcttg caaggccctg ttcctgctgg tagtccagcc ggacaagggg
    49981 acagttatgc ttttgggcca cgcctgcggc ttggaacacg tcgttattca gcttttgctt
    50041 ggtgtcggcc acgttgagga caaggaacgt caactggaac agccgttcat tgcgggtctg
    50101 caactttgtc agcagcttct tggcgtcctc gccgtaggtg gcaaggtcgc tgggaagaat
    50161 gtccatatcg taaccgctgc ggacggcctt tttctgctcc gcaatcttca tagcgtcaag
    50221 gtcggtaatt ttgcgcttga tggtcttgat cgccttggtc tgttcaatgg cctgcacatg
    50281 gagattgact aaaacaccgc tttccgtatt gaggaagtcg gccagcatat cgtccgacag
    50341 ctccggggac aggatttgca ggaaacttac cgcgccgtat ttgccgccca tgccgaatgt
    50401 ctttgcattg ccgaagcaca gggaggacgg ggcaataaaa tccttggtgg agaggccgga
    50461 cggggccagc cacttccagt caaaattgaa tatctcgccg tccgggtggt acacgccatg
    50521 cagcaggttc agccgttccc agccgtccat cacatgggct acggccccca tgctcttgaa
    50581 atagcctaac aggtcaaagc cgatgcgctt caatctggct cgcgccgctt tcacgctttc
    50641 ggcctcgatg gtgaatgtca ggtatttggt ctttaccatg ccgttgttgc cgtttgcaag
    50701 ctgcttttgc agaatgccgg tgtactcggc ccggatgtcg tcgaagtcgt ccccctgcgg
    50761 cgcgatctca aagctcttgg cgtactgcac cgggtcaact ttccgattga ggaaagaaaa
    50821 ctggatatga atggaggcgt ccacatagtt atacaggtcg cacagttttt caaagatagc
    50881 cgtctgcgtg tccggctggg caagctgata gctgatgtcc gcaaactcaa tgcacttgga
    50941 aaagacgccg ggcgataccc ggcacacgcc gtccgggtac atgacctcgt aggggatgct
    51001 ctgctgcgcc gtgtggggcc tgccgtcgcc cttatattta cggattaccg cgtcaatttc
    51061 ttttcgctgg gcgcgggtca gctttgtttt ttcggtttct cttgggattt cctttttgga
    51121 caatggcctt tacctcctgt tccatctggt attgccgcac aagggcggtg taggtgttgt
    51181 cggtctgata gggacgctcc tttggctgga tgaagcagca ctggatgatc tgcataatca
    51241 ccacttcaag gggctggccg tgacgctcgt acatggcaag caaaaagccc ggcagcatgg
    51301 caaataccat gagcagggcc gccgcgctgt tggtaagata gtcacgggtg agaaaatata
    51361 aggggacgcc gatcaccagc gccccggaaa aacagatcag ttgccgtttg gtaagcccaa
    51421 acacaacctt gcttttcaca tgggttaagt ccttggggac ggttacataa gccatttggc
    51481 acctcctttc acgtcaggat aaggtcacat tccacctcgt tcccccacac gtcccagccg
    51541 ggggttttct gtctggcaaa cagctccacg cggggcaaat cgcccgccag ctctacgatt
    51601 ttttcacggg tcacatccgg ctttttgctg tgttgttcaa tcgggctgat aataaactga
    51661 tggacagagg cagaacgacg cttcggcttg ccgtgtgttg ccagcaggca gatttccgcg
    51721 tttccccttg tccagcggcc cagcccgtaa aaccatgtaa ggcttttacg gttctgtttc
    51781 agccatacaa aagccacggt cttgaatgaa aagccccacg ctttgattaa ccgcagagct
    51841 tccggcagca tggggaatgt ggcccacaaa aacagcaggc agtctttgga cgccagccgt
    51901 tccaccggca gggcgcacag ctcgtcaatg ctcatggtgg aatagtgatt ttccgctgcg
    51961 ccctgtacgt ttttgcagtc ataccgccac ggcgggtcag cgtaaatgat gttgtacgtt
    52021 gaaataagca agttcctcct gtgtatgctt ggattttggg atcaatgcgc cccgaatacg
    52081 gcctttgata tactgccggt cttgaacagg cagaagcaca gcagcaccgt atagcccata
    52141 cagccccaga ttgcgccgga tatgtcggaa gcggaaccga tattctgtat cagcaccgcg
    52201 tagataccca ccacaaccat gattaaaaag gcttggaagc ccagcgccag cagggatttc
    52261 agataccctt ggcccatgcc gcgccattcc gcgttgccca ttgtggcaag ggggataggc
    52321 cccagcgacg tgacaacata gatttccacc atgcggccat aggtcacaag gaaaatgcag
    52381 atggaaagga tattcatggt gaggcccaca acaagggttt ggaaccacag ccccaaaaga
    52441 gcgcccacgc tcatagcctc caattggctt tcaaggtctg gtatcagggt gttaaaatca
    52501 atggatgtct gcccgataat cacaccggcg ctttgattga cgacgctttg ggtggcgtcg
    52561 aatacgccca tgacgatgtt ccatgtgttc gttacgatca ggattgcaaa cgcggatttg
    52621 aatatccagc ggaagaacat ggagctgtca aagtcgtgca tattgttctt ttccgttatc
    52681 atctggataa gctcatagca catgacaagg gcgagtatgg ccccggctat ggggacgatc
    52741 acggtttccg acaggctgtg cagcatattg aaaataccgc tgttccatgc ctgcggggtt
    52801 cgtcccacgt cggcggcgat ttctccgact ttggcattta ccgtatcgaa cagcccggac
    52861 aggttgcccg tgataccgtc catgaggatg ccttttatcc attcttcaat gatttcacta
    52921 atcacgggta gcacctcctt tcccgcgccc tcccggtctg gccgggagga cgcggaggcg
    52981 attagttaag ggtttggaca gggacaaagg ccaggatcag cccagcagcc cggacagcag
    53041 gggaaccaga gtgacaccga tcagggccac gccgccgcca gcgacaagct gcttgacacc
    53101 ctgcgatttt gcccccggat tgtcctgccc gtatccctcc aacagattga tgccgcccca
    53161 tacgcacaga gcgccgccca gtccgatcac gatagtctgc aaagtagtaa ctgcggaatt
    53221 aaaaaatgcc ataaatttac ctctttctgc cggtttccgg ctcataatga ttttttgatt
    53281 ttgttgttgg aatgaaaaaa gccgctgttt attcagcggc ggcgtcctcg tccgacaggt
    53341 cgatttcata caggtcgaat acttcatcct gcgttaccac ttgctgccgt ttccggcgcg
    53401 tgatagtcaa aaatctgtcc acgtcaaagg tattcttctt gtcggcgtcg gcaaggtatt
    53461 tgtaccgggg atgcttggta atatcgaatt tctccgataa aaacgggcgc acaccccgta
    53521 gctgcaagat acacttgccg ccgtccatca cggcaatttc gtcttgggac atcaactcct
    53581 ttcctaattt ttgatagttc aatccgtggg acgtttggct tccccggttc tcgctggtgt
    53641 tgaaactgtc aatcgtttct ttccccagcg catcggaaat ctccttggca tttttgccgc
    53701 gcccactaag gaaaagcaca ctatcgcagt tatccgaaat gatctcggcg gcgtccttgt
    53761 aaatcgcttt tagctgcgac tggctctgca aaataataga ggccgatatt tcacggcttc
    53821 gaatggtggc aatgagttta tcgaaattgg gtatttggcc aatgttggca aattcatcca
    53881 aaatcaggcg tacatggaca ggcagccgcc cgccgtacac atcatcagcc cggtcacaaa
    53941 gcaggttgat tagctggctt tggagcatag ccagaatgaa attaaaggtc gtgtccgtat
    54001 cgctcataat gagatacagc accgtctttt cgtccccgat ggtgtccagc tccatttcgt
    54061 cgtcctccat cagctcgcgt acctcccgga tgtcaaaggg cgcaagtctg gcaccgcatg
    54121 aaatcaggat ggaagaacgg gtcttgcctg cggacagcag gaatttcttg tactggcgca
    54181 ccgcaaaatg gtcggggtct ttctgctcca aacgctcaaa catgagatcg acggggcttt
    54241 gaaactccgg gtcgtcctcg cgggcttcac tggcgtttat catttccagc agcgttgaga
    54301 agttcatttc ctccgctggg gcctcgtacc agatatagcc gatcagggcc gaataaaaca
    54361 accgttccga cttcacccaa aaatcctccg ctgacttttc cccctcgccc ttggtgttgg
    54421 aaatcagggt atttaccagc tttagcacgt ccttttccga atggatgtag acaaaggggt
    54481 tgtacttcat ggacttggaa aagttaatgg tattcaggat tttaatgcgg tagcccgcct
    54541 gctggaacat cctgccggtt tccagaacca aagtgccttt cggatctgtc accacaaacg
    54601 aagtcggata gtccgtggtc ggaaagcatt gcatcaggtt gggtttcacg aaaaatctcg
    54661 ttttccctga accgctgcca ccgatcacca gcacgttttt attccgcgct gtcttggggt
    54721 ctttgggcct gccggtcatg gtaagccgtt ccgtttgggt gagcaggata ttattttcaa
    54781 acttggggtc gatgtaaggc gctatgtctt tggccgtccc ccagcgggcg ctgccatact
    54841 ccatgccttt gcggtatttc ttcacgttct tgctcttgca gtacaccacc agccgcacga
    54901 tcacggcacc ggcgataccg atacacaggt caaacaggtg caggcttggc agcgggttgg
    54961 caaaagccgc cgcaaaacca tccgcaaaat gcaggagctt ccccgacagg tcagcaccag
    55021 cggccagccg gtacgcctcc ccgcatttcg caaacaggta cacaaaaatc agatacggga
    55081 ggttcaggat cagcaatttc tttacgtcag gcttcacagt tccagccccc tttccttgat
    55141 tttctccttg gcccgctgct tggtctgggc cgccgccttt tgcttcacag cggacaaaac
    55201 cttgcggata gagggccgct gctccttgga aagcgtcttg gcggtaaact cccgaaacgc
    55261 cagatcaagg ttatctgcat ccttggcctt gaaaatcaca acgtagtggg gcggctgggt
    55321 ggtcttatct tttcgtaagg taaaatccac gccgtatttc ttggcgcatg gcttgaacgc
    55381 tccaatgttg gcctcggtga tctcaatgtt ggagagatca gccccatgct ttttaagctg
    55441 ctttaggctc tgctggccgt ggtgcagcgt gtttttgccc ttggcccggt gttgcaggta
    55501 ctttttcagc gccgcttgca gcacttgagc cgtgagcttg cccgttttca tggacagggc
    55561 aatggtcttt tgagtaactt cttcctgcaa tcgcatccct ccttgtcaga aagtcaaaag
    55621 cggcgtctaa ggcggaacca ccagacgccg caggtagata taggtcaaat ccgcacccgc
    55681 aggccggaaa ggtcgtcggc ccggatgccg acaagatacc agttttcggc catttcaatg
    55741 cgggtgggcc tccatttgcc gccgatcatc acgtcgaaac actccccgca atgcaggccg
    55801 ccgtaatagt cggccagatc aaagcggatg tcgtaacggt ctgttcgctc gtcaaaaatc
    55861 aatgcgccct gcttcatagg gtcgtcctcc ttttctcgtt acaggctttc gccctcgccg
    55921 caggaatagt ccggcaggcc ggatttaggc tttccccaat cgggctgtgc catgtcgtgc
    55981 gcgacaaggg ccgtgtagta gttggaaatg gtgctggggg cgttgaacag catagcccgc
    56041 aggtattgtt tgatattgcg gactttcgtt gtgttctccc gcagacagtc aaggacaaag
    56101 cggatatgtt cgccggtcag cttcatgtac ttggatttca ccagctcggc agggtagtcg
    56161 tccccggcga cacggattgt ctttcgggcg gtgcagacgg tttccagcat caggtctacg
    56221 atttcgtcaa gctgttcacg gtcaatgctc ctgtcctgaa tgagataggc atactcgatg
    56281 ttctccaaaa tgatttcccg ataaatccgt acagcctcat cctttcctgt ccttttccgt
    56341 tccggcggcg tagccgctgc ctcgccctcc ataggggcag gggtcgggga tgggatagga
    56401 atggaatggg tactttgtcc atctgtaatt attttttctt tttttgataa gtcagttctt
    56461 tgtacttctt tatttaattg cgttggattt tccgacgccg gattatccga tgtcggattt
    56521 tccaacaccg ggttatccga tgttggattt tccaataccg ggttttccaa taccggctgt
    56581 tcgtagatgg tatagaccat atcggccatt ttgccttttg cgtcacggcc ctgctcacgc
    56641 cggatatatc cagctttttc aagctcccat accgctgtcc tgatcgcgtc gatgctttcc
    56701 cggttgatat aggacagtcc ttttagggta tagtcccaat cctccggcaa ggacaacatc
    56761 tgcgacagca ggcccttggc ctttaaggtc aggttcttat tccgcaggtg gtgatttgac
    56821 atcaccgtat agcctttgtt cttttccaca cggaaaactg ccatagcgtt gttcgctcct
    56881 ttcactgtga atttgaccgc tgcatagtcg gtcagcttgc ccggctgata gggacactct
    56941 gcaaactggc agaattgata tttccaatcc gggcggtaaa atcggcaggt gccgcaatcc
    57001 tccggcgcgt ccgccccgcc gccgtcgtag tgatcgaaac cgggcttttc ctgcatcagc
    57061 cgttcaaacg cccggtcagg gccggaagta aatagcatgg cgtcgcctcc tttctctatt
    57121 tgggcataaa aaagcggcgt cctgttctcc catgacggga aagaggaacg ccgcgtttac
    57181 ggtgtcgtat tcaatttatg atgtgccgta tttggggtgg cgggtattag cagtatggtt
    57241 cagcatatat gcggctcaat ccgaaagtag taaattggta gtaaattgag ttctcatatc
    57301 ctgcgaaatg cccgtatttc cggggctttc agcgataacc taaatttttg gaataaaact
    57361 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    57421 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    57481 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    57541 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    57601 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    57661 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    57721 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    57781 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    57841 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    57901 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    57961 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    58021 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    58081 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    58141 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    58201 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    58261 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    58321 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    58381 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    58441 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    58501 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    58561 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    58621 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    58681 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    58741 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    58801 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    58861 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    58921 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    58981 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    59041 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    59101 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    59161 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    59221 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    59281 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    59341 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    59401 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    59461 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    59521 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    59581 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    59641 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    59701 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    59761 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    59821 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    59881 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    59941 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    60001 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    60061 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    60121 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    60181 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    60241 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    60301 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    60361 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    60421 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    60481 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    60541 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    60601 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    60661 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    60721 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    60781 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    60841 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    60901 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    60961 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    61021 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    61081 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    61141 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    61201 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    61261 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    61321 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    61381 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    61441 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    61501 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    61561 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    61621 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    61681 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    61741 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    61801 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    61861 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    61921 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    61981 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    62041 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    62101 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    62161 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    62221 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    62281 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    62341 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    62401 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    62461 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    62521 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    62581 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    62641 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    62701 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    62761 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    62821 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    62881 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    62941 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    63001 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    63061 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    63121 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    63181 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    63241 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    63301 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    63361 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    63421 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    63481 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    63541 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    63601 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    63661 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    63721 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    63781 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    63841 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    63901 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    63961 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    64021 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    64081 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    64141 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    64201 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    64261 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    64321 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    64381 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    64441 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    64501 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    64561 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    64621 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    64681 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    64741 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    64801 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    64861 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    64921 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    64981 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    65041 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    65101 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    65161 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    65221 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    65281 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    65341 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    65401 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    65461 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    65521 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    65581 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    65641 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    65701 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    65761 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    65821 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    65881 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    65941 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    66001 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    66061 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    66121 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    66181 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    66241 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    66301 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    66361 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    66421 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    66481 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    66541 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    66601 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    66661 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    66721 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    66781 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    66841 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    66901 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    66961 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    67021 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    67081 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    67141 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    67201 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    67261 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    67321 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    67381 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    67441 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    67501 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    67561 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    67621 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    67681 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    67741 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    67801 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    67861 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    67921 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    67981 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    68041 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    68101 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    68161 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    68221 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    68281 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    68341 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    68401 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    68461 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    68521 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    68581 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    68641 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    68701 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    68761 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    68821 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    68881 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    68941 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    69001 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    69061 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    69121 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    69181 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    69241 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    69301 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    69361 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    69421 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    69481 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    69541 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    69601 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    69661 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    69721 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    69781 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    69841 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    69901 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    69961 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    70021 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    70081 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    70141 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    70201 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    70261 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    70321 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    70381 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    70441 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    70501 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    70561 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    70621 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    70681 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    70741 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    70801 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    70861 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    70921 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    70981 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    71041 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    71101 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    71161 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    71221 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    71281 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    71341 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    71401 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    71461 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    71521 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    71581 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    71641 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    71701 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    71761 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    71821 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    71881 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    71941 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    72001 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    72061 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    72121 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    72181 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    72241 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    72301 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    72361 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    72421 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    72481 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    72541 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    72601 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    72661 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    72721 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    72781 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    72841 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    72901 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    72961 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    73021 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    73081 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    73141 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    73201 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    73261 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    73321 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    73381 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    73441 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    73501 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    73561 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    73621 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    73681 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    73741 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    73801 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    73861 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    73921 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    73981 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    74041 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    74101 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    74161 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    74221 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    74281 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    74341 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    74401 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    74461 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    74521 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    74581 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    74641 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    74701 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    74761 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    74821 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    74881 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    74941 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    75001 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    75061 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    75121 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    75181 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    75241 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    75301 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    75361 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    75421 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    75481 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    75541 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    75601 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    75661 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    75721 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    75781 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    75841 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    75901 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    75961 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    76021 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    76081 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    76141 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    76201 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    76261 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    76321 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    76381 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    76441 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    76501 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    76561 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    76621 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    76681 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    76741 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    76801 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    76861 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    76921 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    76981 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    77041 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    77101 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    77161 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    77221 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    77281 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    77341 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    77401 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    77461 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    77521 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    77581 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    77641 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    77701 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    77761 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    77821 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    77881 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    77941 aagtctctta ttttaaattg aatacattat aatgat
//
LOCUS       DACCWV010000003        64730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCWV010000003 DACCWV010000000
VERSION     DACCWV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.003"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(65..730)
                     /locus_tag="IVW84_000172"
     CDS             complement(65..730)
                     /locus_tag="IVW84_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916403.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(877..1482)
                     /gene="dhaL"
                     /locus_tag="IVW84_000173"
     CDS             complement(877..1482)
                     /gene="dhaL"
                     /locus_tag="IVW84_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7916404.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1510..2493)
                     /gene="dhaK"
                     /locus_tag="IVW84_000174"
     CDS             complement(1510..2493)
                     /gene="dhaK"
                     /locus_tag="IVW84_000174"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7916405.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(2495..2890)
                     /gene="dhaM"
                     /locus_tag="IVW84_000175"
     CDS             complement(2495..2890)
                     /gene="dhaM"
                     /locus_tag="IVW84_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7916406.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3044..3364
                     /locus_tag="IVW84_000176"
     CDS             3044..3364
                     /locus_tag="IVW84_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7916407.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3476..4345)
                     /locus_tag="IVW84_000177"
     CDS             complement(3476..4345)
                     /locus_tag="IVW84_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7916408.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(4441..5745)
                     /locus_tag="IVW84_000178"
     CDS             complement(4441..5745)
                     /locus_tag="IVW84_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7916409.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5742..6251)
                     /locus_tag="IVW84_000179"
     CDS             complement(5742..6251)
                     /locus_tag="IVW84_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916410.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6426..8561)
                     /locus_tag="IVW84_000180"
     CDS             complement(6426..8561)
                     /locus_tag="IVW84_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7916411.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8644..10299)
                     /locus_tag="IVW84_000181"
     CDS             complement(8644..10299)
                     /locus_tag="IVW84_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7916412.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10301..10615)
                     /locus_tag="IVW84_000182"
     CDS             complement(10301..10615)
                     /locus_tag="IVW84_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7916413.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10785..11045
                     /locus_tag="IVW84_000183"
     CDS             10785..11045
                     /locus_tag="IVW84_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7916414.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11059..11373
                     /locus_tag="IVW84_000184"
     CDS             11059..11373
                     /locus_tag="IVW84_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7916415.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12103..12657)
                     /locus_tag="IVW84_000185"
     CDS             complement(12103..12657)
                     /locus_tag="IVW84_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7916416.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12783..14712)
                     /locus_tag="IVW84_000186"
                     /pseudo
     CDS             complement(12783..14712)
                     /locus_tag="IVW84_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15018..15734)
                     /locus_tag="IVW84_000187"
     CDS             complement(15018..15734)
                     /locus_tag="IVW84_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7916417.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15735..17558)
                     /gene="glpO"
                     /locus_tag="IVW84_000188"
     CDS             complement(15735..17558)
                     /gene="glpO"
                     /locus_tag="IVW84_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7916418.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17572..19068)
                     /gene="glpK"
                     /locus_tag="IVW84_000189"
     CDS             complement(17572..19068)
                     /gene="glpK"
                     /locus_tag="IVW84_000189"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7916419.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19204..20664)
                     /locus_tag="IVW84_000190"
     CDS             complement(19204..20664)
                     /locus_tag="IVW84_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7916420.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20654..21949)
                     /locus_tag="IVW84_000191"
     CDS             complement(20654..21949)
                     /locus_tag="IVW84_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7916421.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22281..22958)
                     /locus_tag="IVW84_000192"
     CDS             complement(22281..22958)
                     /locus_tag="IVW84_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7916422.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22988..24079)
                     /locus_tag="IVW84_000193"
     CDS             complement(22988..24079)
                     /locus_tag="IVW84_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7916423.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24180..24614)
                     /locus_tag="IVW84_000194"
     CDS             complement(24180..24614)
                     /locus_tag="IVW84_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7916424.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25198..26655)
                     /gene="ascB"
                     /locus_tag="IVW84_000195"
     CDS             complement(25198..26655)
                     /gene="ascB"
                     /locus_tag="IVW84_000195"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7916425.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26652..28547)
                     /locus_tag="IVW84_000196"
     CDS             complement(26652..28547)
                     /locus_tag="IVW84_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7916426.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28605..30380)
                     /locus_tag="IVW84_000197"
     CDS             complement(28605..30380)
                     /locus_tag="IVW84_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7916427.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30430..31926)
                     /locus_tag="IVW84_000198"
     CDS             complement(30430..31926)
                     /locus_tag="IVW84_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7916428.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32120..33742)
                     /locus_tag="IVW84_000199"
     CDS             complement(32120..33742)
                     /locus_tag="IVW84_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7916429.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34070..35383)
                     /locus_tag="IVW84_000200"
     CDS             complement(34070..35383)
                     /locus_tag="IVW84_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7916430.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35628..35852)
                     /locus_tag="IVW84_000201"
     CDS             complement(35628..35852)
                     /locus_tag="IVW84_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916431.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35872..36240)
                     /locus_tag="IVW84_000202"
     CDS             complement(35872..36240)
                     /locus_tag="IVW84_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7916432.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(36436..37146)
                     /locus_tag="IVW84_000203"
     CDS             complement(36436..37146)
                     /locus_tag="IVW84_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916433.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37164..37661)
                     /locus_tag="IVW84_000204"
     CDS             complement(37164..37661)
                     /locus_tag="IVW84_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7916434.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37698..38150)
                     /locus_tag="IVW84_000205"
     CDS             complement(37698..38150)
                     /locus_tag="IVW84_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7916435.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38285..38890)
                     /locus_tag="IVW84_000206"
     CDS             complement(38285..38890)
                     /locus_tag="IVW84_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7916436.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39340..39960)
                     /locus_tag="IVW84_000207"
     CDS             complement(39340..39960)
                     /locus_tag="IVW84_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7916437.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39962..40831)
                     /locus_tag="IVW84_000208"
     CDS             complement(39962..40831)
                     /locus_tag="IVW84_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7916438.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40854..41261)
                     /locus_tag="IVW84_000209"
     CDS             complement(40854..41261)
                     /locus_tag="IVW84_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7916439.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41280..42569)
                     /locus_tag="IVW84_000210"
     CDS             complement(41280..42569)
                     /locus_tag="IVW84_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7916440.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42596..42898)
                     /locus_tag="IVW84_000211"
     CDS             complement(42596..42898)
                     /locus_tag="IVW84_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7916441.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42923..45016)
                     /locus_tag="IVW84_000212"
     CDS             complement(42923..45016)
                     /locus_tag="IVW84_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7916442.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45505..46269)
                     /locus_tag="IVW84_000213"
     CDS             complement(45505..46269)
                     /locus_tag="IVW84_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7916443.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46380..47249)
                     /gene="mdcB"
                     /locus_tag="IVW84_000214"
     CDS             complement(46380..47249)
                     /gene="mdcB"
                     /locus_tag="IVW84_000214"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7916444.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(47274..48350)
                     /locus_tag="IVW84_000215"
     CDS             complement(47274..48350)
                     /locus_tag="IVW84_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7916445.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(48374..48967)
                     /locus_tag="IVW84_000216"
     CDS             complement(48374..48967)
                     /locus_tag="IVW84_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7916446.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48971..50647)
                     /gene="mdcD"
                     /locus_tag="IVW84_000217"
     CDS             complement(48971..50647)
                     /gene="mdcD"
                     /locus_tag="IVW84_000217"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7916447.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(50637..50936)
                     /locus_tag="IVW84_000218"
     CDS             complement(50637..50936)
                     /locus_tag="IVW84_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7916448.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(51038..52684)
                     /gene="mdcA"
                     /locus_tag="IVW84_000219"
     CDS             complement(51038..52684)
                     /gene="mdcA"
                     /locus_tag="IVW84_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP7916449.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(52691..53602)
                     /locus_tag="IVW84_000220"
     CDS             complement(52691..53602)
                     /locus_tag="IVW84_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7916450.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            53722..54579
                     /locus_tag="IVW84_000221"
     CDS             53722..54579
                     /locus_tag="IVW84_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7916451.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(54766..55128)
                     /locus_tag="IVW84_000222"
     CDS             complement(54766..55128)
                     /locus_tag="IVW84_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916452.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            55474..56913
                     /locus_tag="IVW84_000223"
     CDS             55474..56913
                     /locus_tag="IVW84_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7916453.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(56920..59472)
                     /locus_tag="IVW84_000224"
     CDS             complement(56920..59472)
                     /locus_tag="IVW84_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7916454.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(59586..60917)
                     /locus_tag="IVW84_000225"
     CDS             complement(59586..60917)
                     /locus_tag="IVW84_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7916455.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60883..62361)
                     /locus_tag="IVW84_000226"
     CDS             complement(60883..62361)
                     /locus_tag="IVW84_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7916456.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(62666..64501)
                     /gene="tdc"
                     /locus_tag="IVW84_000227"
     CDS             complement(62666..64501)
                     /gene="tdc"
                     /locus_tag="IVW84_000227"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7916457.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        18443 a        13881 c        10976 g        21430 t
ORIGIN      
        1 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
       61 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
      121 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
      181 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
      241 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
      301 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
      361 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
      421 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
      481 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
      541 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
      601 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
      661 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
      721 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
      781 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
      841 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
      901 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
      961 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
     1021 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
     1081 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
     1141 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
     1201 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
     1261 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
     1321 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
     1381 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
     1441 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
     1501 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
     1561 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
     1621 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
     1681 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
     1741 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
     1801 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
     1861 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
     1921 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
     1981 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
     2041 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
     2101 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
     2161 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
     2221 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
     2281 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
     2341 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
     2401 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
     2461 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
     2521 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
     2581 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
     2641 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
     2701 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
     2761 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
     2821 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
     2881 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
     2941 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
     3001 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
     3061 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
     3121 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
     3181 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
     3241 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
     3301 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
     3361 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
     3421 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
     3481 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
     3541 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
     3601 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
     3661 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
     3721 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
     3781 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
     3841 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
     3901 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
     3961 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
     4021 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
     4081 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
     4141 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
     4201 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
     4261 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
     4321 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
     4381 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
     4441 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
     4501 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
     4561 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
     4621 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
     4681 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
     4741 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
     4801 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
     4861 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
     4921 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
     4981 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
     5041 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
     5101 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
     5161 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
     5221 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
     5281 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
     5341 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
     5401 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
     5461 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
     5521 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
     5581 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
     5641 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
     5701 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
     5761 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
     5821 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
     5881 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
     5941 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
     6001 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
     6061 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
     6121 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
     6181 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
     6241 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
     6301 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
     6361 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
     6421 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
     6481 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
     6541 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
     6601 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
     6661 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
     6721 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
     6781 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
     6841 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
     6901 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
     6961 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
     7021 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
     7081 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
     7141 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
     7201 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
     7261 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
     7321 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
     7381 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
     7441 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
     7501 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
     7561 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
     7621 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
     7681 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
     7741 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
     7801 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
     7861 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
     7921 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
     7981 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
     8041 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
     8101 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
     8161 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
     8221 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
     8281 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
     8341 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
     8401 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
     8461 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
     8521 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
     8581 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
     8641 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
     8701 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
     8761 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
     8821 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
     8881 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
     8941 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
     9001 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
     9061 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
     9121 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
     9181 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
     9241 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
     9301 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
     9361 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
     9421 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
     9481 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
     9541 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
     9601 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
     9661 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
     9721 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
     9781 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
     9841 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
     9901 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
     9961 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    10021 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    10081 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    10141 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    10201 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    10261 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    10321 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    10381 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    10441 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    10501 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    10561 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    10621 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    10681 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    10741 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    10801 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    10861 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    10921 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    10981 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    11041 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    11101 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    11161 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    11221 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    11281 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    11341 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    11401 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    11461 aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    11521 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
    11581 gaagaggttg tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc
    11641 tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    11701 aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
    11761 gatttctaca tttttgcata aatttggctt aataccgaga aactggcttt tgaataccgt
    11821 ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa
    11881 atagctgctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    11941 gtttaccaag aggttgtgag cctaccattt aactccgaat cacaagaaat aatttcaaaa
    12001 acaacttctc ctgtttttgg ttcggttggc ttattatcga gaagtagcta ctgggagtct
    12061 atttatcaaa aaaaggaatg cgacataggt cacacccctt ttttattttc tttgaaacat
    12121 ctttcggtac tcgacagcat acaagacaaa agattgtaaa agaccgtaag tgatgacggc
    12181 aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc
    12241 aacatagatg acagcagtcg ctactttgcc ataccacttc gctccactta atttgatatt
    12301 tttagatagc ccgattaatc cagctacaaa cataaagcct tcttttatca agaatagacc
    12361 aaacaacagc caaagccacg gtcgatacca tgccatagac aaaatcagaa aaagttgtgt
    12421 caatttatct gcaaacggat caagcacctt ccctaagtct gttacctgat tatatctccg
    12481 agcaatgacc ccgtctagat aatcagacaa acctgagaca acaatgaccg ctcctgctat
    12541 ataaaatgac tgtctcagca caaaataaag ataaatcggc agcaaaaaaa tacggaatat
    12601 cgataaaaga ttcggaatcg tttgtatttc tttacgccag tctgtgctta atttcatttt
    12661 atcgccctac ctcctcagca ctcttttctt tttattttag cttacacggg gaataaaacc
    12721 aatgatttca ctgtttttat ttttctttac agacactgat attatgagct aatttgagat
    12781 aatcacttgc tgttttcatc gtttactctt ctttcttcct gtcgtttgcg catttctttt
    12841 actagtactt tatgagcttt ttttgatcct cgctctaggt cacgctgccg tttacgtgtt
    12901 ttataatcga taaacagact gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa
    12961 cgcaccagac gaacacgctg acgaggcacg tggattattt ttttgtctaa atatacatca
    13021 atcgtatctt cacctatatc ttcataaact aacgcttctt tttggtattc attgacaaat
    13081 atccgatcac cttttgcaaa gagctgacgt tcttttttat tctccttcct atttgtaact
    13141 gctgtcttag gaagaaattc ttttttctgt gtccgatagg ctttttccgc caagtaacgc
    13201 tctgcttgat ggatcaattt catcgacata tccattttat gagcaatcca caaagcttgg
    13261 ctttctcctg tttcttccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
    13321 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
    13381 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
    13441 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
    13501 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
    13561 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
    13621 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
    13681 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
    13741 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
    13801 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
    13861 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
    13921 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
    13981 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
    14041 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
    14101 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
    14161 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
    14221 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
    14281 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
    14341 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
    14401 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
    14461 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
    14521 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
    14581 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
    14641 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
    14701 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
    14761 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
    14821 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
    14881 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
    14941 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
    15001 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
    15061 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
    15121 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
    15181 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
    15241 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
    15301 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
    15361 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
    15421 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
    15481 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
    15541 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
    15601 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
    15661 acaccatctc caagcaaaac taggatcagt gtcccaataa actcccccat gatttgagtc
    15721 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
    15781 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
    15841 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
    15901 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
    15961 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
    16021 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
    16081 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
    16141 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
    16201 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
    16261 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
    16321 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
    16381 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
    16441 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
    16501 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
    16561 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
    16621 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
    16681 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
    16741 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
    16801 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
    16861 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
    16921 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
    16981 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
    17041 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
    17101 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
    17161 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
    17221 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
    17281 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
    17341 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
    17401 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
    17461 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
    17521 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
    17581 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
    17641 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
    17701 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
    17761 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
    17821 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
    17881 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
    17941 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
    18001 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
    18061 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
    18121 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
    18181 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
    18241 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
    18301 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
    18361 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
    18421 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
    18481 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
    18541 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
    18601 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
    18661 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
    18721 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
    18781 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
    18841 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
    18901 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
    18961 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
    19021 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
    19081 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
    19141 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
    19201 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
    19261 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
    19321 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
    19381 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
    19441 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
    19501 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
    19561 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
    19621 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
    19681 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
    19741 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
    19801 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
    19861 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
    19921 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
    19981 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
    20041 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
    20101 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
    20161 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
    20221 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
    20281 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
    20341 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
    20401 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
    20461 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
    20521 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
    20581 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
    20641 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
    20701 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
    20761 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
    20821 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
    20881 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
    20941 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
    21001 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
    21061 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
    21121 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
    21181 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
    21241 cgggacgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
    21301 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
    21361 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
    21421 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
    21481 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
    21541 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    21601 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    21661 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    21721 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    21781 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    21841 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    21901 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    21961 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    22021 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    22081 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    22141 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    22201 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    22261 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    22321 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    22381 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    22441 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    22501 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    22561 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    22621 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    22681 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    22741 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    22801 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    22861 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    22921 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    22981 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    23041 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    23101 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    23161 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    23221 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    23281 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    23341 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    23401 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    23461 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    23521 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    23581 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    23641 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    23701 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    23761 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    23821 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    23881 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    23941 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    24001 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    24061 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    24121 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    24181 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    24241 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    24301 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    24361 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    24421 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    24481 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    24541 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    24601 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    24661 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    24721 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    24781 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    24841 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    24901 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    24961 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    25021 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    25081 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    25141 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    25201 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    25261 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    25321 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    25381 taaatctact ccatctttga tagcttcctt catctgctca atatgctgct tcaaatagtc
    25441 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    25501 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    25561 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    25621 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    25681 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    25741 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    25801 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    25861 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    25921 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    25981 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    26041 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    26101 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    26161 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    26221 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    26281 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    26341 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    26401 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    26461 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    26521 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    26581 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    26641 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    26701 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    26761 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    26821 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    26881 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    26941 ttcgttggaa aaactgttga aacttccccg tttacagaac tgtagatttt tccgtccgtt
    27001 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    27061 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    27121 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    27181 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    27241 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    27301 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    27361 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    27421 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    27481 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    27541 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    27601 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    27661 tgacttgcat aatctgcaat tggacctaca actaataaaa taattgggac catcacgatg
    27721 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    27781 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    27841 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    27901 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    27961 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    28021 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    28081 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    28141 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    28201 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    28261 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    28321 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    28381 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    28441 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    28501 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    28561 cttctaatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    28621 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    28681 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    28741 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    28801 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    28861 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    28921 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    28981 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    29041 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    29101 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    29161 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    29221 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    29281 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    29341 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    29401 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    29461 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    29521 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    29581 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    29641 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    29701 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    29761 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    29821 ggaaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    29881 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    29941 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    30001 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    30061 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    30121 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    30181 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    30241 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    30301 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    30361 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    30421 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    30481 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    30541 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    30601 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    30661 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    30721 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    30781 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    30841 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    30901 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    30961 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    31021 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    31081 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    31141 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    31201 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    31261 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    31321 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    31381 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    31441 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    31501 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    31561 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    31621 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    31681 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    31741 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    31801 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    31861 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    31921 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    31981 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    32041 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    32101 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    32161 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    32221 cacgtggaat agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    32281 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    32341 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    32401 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    32461 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    32521 taggcgtcct agcattgtct cttcctttcg ccttgattga ttccaagatt tcctcttttg
    32581 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    32641 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    32701 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    32761 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    32821 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    32881 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    32941 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    33001 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    33061 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    33121 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    33181 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    33241 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    33301 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    33361 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    33421 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    33481 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    33541 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    33601 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    33661 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    33721 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    33781 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    33841 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    33901 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    33961 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatataaga aactattttt
    34021 tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt tatttcacat
    34081 tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt ggagatactt
    34141 cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc
    34201 catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa
    34261 tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa
    34321 tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc
    34381 ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga
    34441 taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag gaacccgcaa
    34501 gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca
    34561 tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa
    34621 gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta
    34681 atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg
    34741 gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg aagatgccac
    34801 ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag
    34861 tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc
    34921 caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa atcgcatatt
    34981 tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa
    35041 taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc
    35101 ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg
    35161 gataacgggc taataccccc attaaaacac agccgatcgc actggctaat gcagtcgcag
    35221 taaacactgc tccttcatcc attccagaag cacctaatac agttggattt acaaataaga
    35281 tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt
    35341 tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat
    35401 ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta
    35461 tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca aaatccgaac
    35521 gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt ggattacaag
    35581 aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca atagtgtgat
    35641 ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc gacaggattc
    35701 agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt tgtcttcttt
    35761 tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg ccgatacttc
    35821 aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta ttcaatcaga
    35881 cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa
    35941 gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa
    36001 ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg aaaacttctg
    36061 cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga gtacaatact
    36121 cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt tttctacaaa
    36181 gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt tttgtatcat
    36241 catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt ccattatatc
    36301 acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca atagtttcta
    36361 ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt
    36421 cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg tgtgtctaac
    36481 actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc
    36541 acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt gtgtttcttc
    36601 cgtcagcatc gttctttggg cttcacgcca gttgagacag cgacagatcg ctccttgttc
    36661 atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag
    36721 gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct tttcgatatc
    36781 ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat ctacaagcgg
    36841 attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt caatagaaga
    36901 acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag caatgacttc
    36961 gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg cctcattcaa
    37021 taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac catttacgga
    37081 taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca ctattacttt
    37141 tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata gtctgtttgg
    37201 ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt tttacgataa
    37261 aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt tttttgcttt
    37321 tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg atgtttaaat
    37381 ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc ctcagattga
    37441 ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact cactaggaaa
    37501 aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata agcttgtgtc
    37561 aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt aaatgtttcg
    37621 atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat ttcgtattta
    37681 ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa attccaatct
    37741 tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc ttcttcgtca
    37801 gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat gaaaggatag
    37861 atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc tttatcttcc
    37921 aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt cgttgtacga
    37981 tctactttga tcatttctgc tagtttttct tggatgattc caggattttc gcaaatacgt
    38041 actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat attgctaata
    38101 gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat ttcatcccac
    38161 ctttattttg tttgtaagac tagttcacct ctttttgttg caaatgcaac tattttcaat
    38221 aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc ttttacgaaa
    38281 aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt ttcttcgttg
    38341 ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg ttggaagttt
    38401 cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa acaagtttat
    38461 aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact gctcgcaact
    38521 gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca ttttcttcag
    38581 gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc cgtttatctt
    38641 cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact tttggtgttg
    38701 gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct tttcctattt
    38761 cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta tcaaatacat
    38821 cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat tgagccaact
    38881 tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat caagtataga
    38941 agactggttt aattacggca aataaatata atgaaatctg caataaataa aagaatgtta
    39001 aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat ggcgttcaaa
    39061 agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct attcttgtct
    39121 ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact aaacggtgtt
    39181 ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa gctgtttttt
    39241 tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc ggtaaacagc
    39301 gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt cattgatttc
    39361 tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg ttacatcaaa
    39421 ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga tctttttgaa
    39481 tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt caccatagtg
    39541 ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt tgtaccgacg
    39601 ttgccataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat atccgtatct
    39661 atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact cggacacaat
    39721 cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag gataaacttt
    39781 cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt ctgcatagta
    39841 ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc gtagtttatt
    39901 cataagatat gtttttcctg taccaggact accagccaga agaatgaaat actttttcat
    39961 ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca atcttatgct
    40021 tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga gacttgttga
    40081 tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa agctccgttc
    40141 caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct tctttcacac
    40201 ctgttcctcc atggataact agggggacat caactactgc atcgatttct ttcagcaagg
    40261 gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata gcaatagcta
    40321 atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt gtatatacct
    40381 tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca agttctcctt
    40441 ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc aggatatttt
    40501 ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg tcgattgctt
    40561 ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca accatttctt
    40621 cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat ttagcggtat
    40681 cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct agcatagcag
    40741 gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac tcttctgctg
    40801 tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc tttttatttg
    40861 tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt gcccatgtag
    40921 taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg gttatagagg
    40981 ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact cttttctaaa
    41041 tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac ttgttcataa
    41101 agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc
    41161 tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt tgccagaact
    41221 tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc tactttcaat
    41281 taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc ggctactgta
    41341 tctacatcgg ttgctgtagc attgttgaat cccatcgaat taaagatcgt tactagcaaa
    41401 gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc tcctcggcgt
    41461 ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt ggttagcata
    41521 ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat aatcgtacca
    41581 atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa gaaaactgga
    41641 caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg aaatgccggc
    41701 acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc cactacgaat
    41761 tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa ttcagaagcg
    41821 aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac gaccatgaca
    41881 gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac
    41941 tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa taagtacccg
    42001 atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg aatgatcggt
    42061 tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc gaccaagatc
    42121 aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat
    42181 aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc caaaataata
    42241 ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc caaatcattc
    42301 attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg aaatccttca
    42361 gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc ggcactcaac
    42421 acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc tgatttcttc
    42481 tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg agtcaaaata
    42541 ttttcgatga taaaattgat aacgcccatg tttttaccct cttttctttt tgtatttaga
    42601 taactccctt atcttgcaga actggggtga ttttttcttc aatttcattt tttgaaacaa
    42661 tatttttcaa atagatcatc gccgttttag gatcaactgc gaacttttcg aactgcgaac
    42721 gaaaattttc tgctgtgatg atcacatctg gtttcatgga aactgcggaa gaaatatcac
    42781 aatggtccag tttggcttct actcctaatt tgttcaaaac atcctcagca gacatctgtg
    42841 ctgcaaaact acttcctaaa cctgcaccgc atacgaataa aatgttcaat ttgttcatct
    42901 ttgttctccc ccgtatcatt tattatttct caactactga aaaaagcagt tgtttgaaat
    42961 cttcgatatt tgtgcattca atcaatcgat ccaacttctc ttcgttgttg atcaattcaa
    43021 ttagttcttt catgatgttc agatgcgagt aggagtctac tgctgccaaa caaaaaataa
    43081 tctttactgg atccatttca ggattaccga aatcgattgg ttgctcaatc gtagctacgc
    43141 tgactcctaa tttattgacc ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc
    43201 caatcacgat atacggtccg taatcattca ctgcacgaat catcgcttcg acatagcgct
    43261 cttcgacaat gttttcttta accaatggtc tagcagctag ttcaatcgat tcctcccaag
    43321 tagtggcttt ttctttgatc atgatattgc tgtccgtcaa aatatctttt aacataggct
    43381 ggatctccct tttgttgatt tctaaattgt ttgtttcgaa taatttttct aacttgccat
    43441 agatgattcg atctactcgg ccaccacttt ctttcatgac atctattatt tgagtgaata
    43501 actccgtcga ttcatgactg ttaggctcta accgctgtag ttcttcattt cttttcaaaa
    43561 agtcttctat aaccaagata ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct
    43621 tatttcgata attcaattga aaggtgctga aaaccagatt gacatccaat ttatctacta
    43681 attcgatttc ttttgaactt aaaactgcag taatctcgat ctgaggaaac ttttctttca
    43741 gattctctgc taacagattt ccagtagcca tcccatggtt acagacaacg actgatttat
    43801 aaacataatt caagtctcgc ttgattacac tggcgatcgt cgaaaagtgg atgacaagaa
    43861 aagctatctc gtcctccagt atcttgccac caattacttc ttcgatcgta ggaacaaaat
    43921 gagtgactgc gcggtagatt gggaaataat ttgttcggat gttttctttc aatggattca
    43981 tgacttggat atgattcttg attcttacaa tcagtgcagc catgtgctta tagagatttt
    44041 cgaataaagc ttcttccttt agatggaaag gtacgtgtgt ctcgttctct acaaattgga
    44101 tcaattggat cgttagtaat tgagcttgta cccactcgat tgaattggag atatctctag
    44161 cattgaacgt ctcaatagtg aaacgaagat aatttctttc tacttgcggg acattgtcca
    44221 attgaaattc ctcgatcatg tggtcaataa atgcagaaaa gctactgtct tttatttctg
    44281 tatttttcca attaatggca gagataaatt catgtttcct gattcgctgg atccagatca
    44341 atgtaaaaag caggatttgt tttttataaa tatcatcttc ttgtttcaac aacttattgg
    44401 aatagatctc ctgtattttt ttgatttcca ccaaaggaaa ataatggaga aagatttttt
    44461 gagccggtgt taattgtgca tcttgattca taaagaagtc ggtcattccc agattctgat
    44521 tgataaaatc atagatcatc gtacggatag atcgttctga acctttataa accagtcctt
    44581 gctttccata actgacgatc tctatcccat aactgaccag tgttgctcgt aaccgtctca
    44641 tatcttcatc taatgtactt ttagagattt gaaattcttc ttcttttttg tttagatgga
    44701 tcgggattcg gctgtaagca acggctaaaa ctagatcaaa aatacgttcg cgccgattca
    44761 aaaagccgtc tttcacatct tttatttcag tttctaatcg agcttgcttt tccttttcca
    44821 gcttcaacaa aaaacctttg ttgcgaattg tacggatctc cggaagcttt ttactgctca
    44881 accagatatt cgcttcaaga acttcctgac ggatcgtccg ctgactgaca ttgaactcac
    44941 ttgctagatg ttttgctttt actggatatt gggctgtcat gattctgtga agtatttcaa
    45001 ttgttcttgt tttcatagaa atgatctcct tctgatttca agtatatggt tccgcttaca
    45061 taataaaaag tagatatttc tgccacttga agtggaaaga aagtaagcaa agcaaacaaa
    45121 aaataagcca tatgcttttt catctcctca acgaaaaaga catacgccta ttattacaaa
    45181 aattttgccc atctatacaa aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    45241 gaacaactaa actaaaatag tgcttcctat ttttcgtttg gttgttctta ttatcgcaga
    45301 tgcaagcttt taaacaccat ttagtggaga ggttgtgaca gagatgttca gccttgagcg
    45361 ataagaagcg attttgaaaa atcgcttccc atattttaca aaatcgtgcc ttattgccga
    45421 taggctatct ctggcacacc gtttattcag aaaaaggagc tgcgacaagg cttttgtcac
    45481 aactccgttc ataaatattt cctgtcaatc cagcataatc ggctgaataa aatcggcttg
    45541 ccatcttttt tctttctcag aaaaatcaaa ataccatatc tgagaaaaat actgtcgttg
    45601 tgtcttactt cctgagatca atcgcccatc ttttcgatcg actgaataat cacagcatct
    45661 gaaatcaatt cggatggcaa aactattttc gtggatctgt cgataatttc ctacttcctg
    45721 aatagttatt tttttagtat gatttcttac accgtctgct ttgttttttt gcagaacttt
    45781 ctgatgttcg gcaaacagct tgtctgtata acagcttctg gctttttcag gagtttcttc
    45841 ttcccaagcg gattggatct tcatgaatat cttttggatt tccagaaact gtgctttctt
    45901 ttctttagcc gttcctgaca tgcgggacaa tatctccgct ttcagccttt tcttttgtct
    45961 tttattcttg acgatagaaa aagcagaaac ccctcctact gacaaaacag ctaatgcaag
    46021 aaatcccgtt tgctcataac tagaaggacc tgtacctgga ccataatacc cataagaatc
    46081 tctgccgtaa gaacgactgc caaaataact gcccccatat gaacgaccac caaatgaacc
    46141 tctgcccgaa cttcgaccgc tagatgtacc tccaccatga ccacccgcga cagcgaatcc
    46201 ttgggtcggc agagcaaaca aacataggcc cactaacaaa atgattgcta aataattgat
    46261 ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa ctatttttat gatagccttg
    46321 ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt tttagcaatt ttgtcttatt
    46381 tagtcattga gtaacatcgg acaaatgtat gtgtcggcag tccataattc ctctatcttc
    46441 agcatgaaca agcttgcaga caagagatct gcgcttcctc caggagagat ttggttttcc
    46501 tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta aagcactttg actagcagca
    46561 tgagcaagtt tttgcatttc cactaacgtt ttctgatcac tgcgatgcaa tacgcatgtg
    46621 tcatctagcg aactcatcaa aactaacaac atatgcattt gtttctgtac atatgaatca
    46681 ttatctagct ccttagctgc ttgaattgca agcctgatat gcggataccc taatatcgct
    46741 tcttcatatg caccatttac gccatatttt tgcttcaccc tatgaccatg agtggttttt
    46801 ttatgacgat attttgtatc aggaaaagca gcaagcttgc tgacatcatc aagtattttc
    46861 gataaagaaa aaggctgttc catactgatt ttttgtgcaa tgacactgat caatagcccc
    46921 attgcccaaa tcgctccttt atgggtattg atgccatttg tagcaatcag cattgccttt
    46981 tccgcttttc gcccaatctc agcaattctt tctcttaact cctgatcaat agcatgatgc
    47041 catgctattt gagccatttc ttcaaaaaaa ggagttagcg tcttagcaga cttaagaaat
    47101 aaagcatagt ccatatcgga atgtgcaccc gcatccagtg catccactaa tccaggtttt
    47161 ggacttaatg ttacttctgc gatcaatgct tcctctacca acgcagcgag ttgctgagtc
    47221 actgcttttt ttcgttcgtc atttatcatg acactcactc cttcttccta ctattacttg
    47281 ctgaaacctt ttttgatagc tacccaaatg atatgaagta accagcttaa agcaaatgca
    47341 caacttgccg tcattagata agctacaaga atcatgactc ctgaaggtaa ctgcagccaa
    47401 ctatctttaa acaataataa cagtacttgg aaaacaaata cattggctaa atacgtacga
    47461 taagcataaa cagacaacca tttgaacaat ggcaattttg gcgaatcgtt tacaatcatt
    47521 ttggaagcgc ctatgaagac agcaaagatg atgactaaac tgtataaact ttggatcgta
    47581 ttcaagtaag gtgcattcgc aaagctcaag tctcctggat gtgcgagcaa tgaacggaca
    47641 gaaaagaacg cgataactaa ggcaacaggt aagaaaagat aacgtactct gtccaaatat
    47701 tgatagactt tttcgtgata aacaaatgcg gctaccccat aaatcatgaa actagcaaca
    47761 aaccgatcta ataagtacca gctgtcatga taaggcccag tcaatacttg tgtttgatag
    47821 aacagatacc atgcgacata taaaatcgtt gaaccaatca atactggcca tacatatttt
    47881 ttattagcaa atactttgta accgagccaa acaaaaaacg gcatgaacaa ttggatctgc
    47941 agcatcataa cggtatacca taaatgaggc gctccatcac ctgtgacata tttcagtaaa
    48001 aagcttgcag tatccgtata cggagtttgt tgttggactg ctggaaatag ccaaagataa
    48061 gccgtcgtcc atagaagata cggaacgact agttcaaaaa acttttcttt caaaaattcc
    48121 caataagaca aatgctgact tgtttttacc atattataaa caattgcaaa aataaacatt
    48181 ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa aagctgtatt tgttgcgctg
    48241 tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca taacagccgt tacagctgat
    48301 actttctgga agtcacttac gttgattttg atttttttgt tttcaaacat ctctttctct
    48361 ctctttttct ttattaccaa agttctttgg ttaatttagg tccttcgact gttttcaata
    48421 gaattttttt ctctggctct ctcaaatact cttttagtga aaaaccgcct tgccgagtca
    48481 ctacttgggt atcgaattgc tcaaaaaaag caggatgcga ttgaattacc tgaattggca
    48541 attcaatcgg tgaatttgcg tacagcagca aatcaaaatc actggtttct tttactgccg
    48601 gaatcccagt cgataattca aatcctaagc tgccgccgat tccccattta catcctgata
    48661 atatgtttct agcagcatga aattgctgat aaacaggata atccatgatt ttctcttgtc
    48721 tatacgattc ttgttcgatg atgtcccacg gatgaatgac ttgtttgacg gcatgttttg
    48781 agatttctgt tgaccatctc tgattgcggg caggtccacg taaaccaatc ggtagtattt
    48841 ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat cttttcagtt agaacgaccc
    48901 attcaggcaa ggtgaaccct tctatttcat gtttatcaaa aaggacaata tcatgtgctt
    48961 gaatcatttt ttatgcatcc cattcttcgt ccattttggc acggacttta tttgtttcga
    49021 cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt tgtcagcatc gtgtcatttg
    49081 tattgcggac atcttgaatg gcttcattga ttgcttcgat gactttgctg acattttgtt
    49141 ctgaagcagc ttggtcggtg atatcttcta ggaaatgata gatcgctcct agttttgtat
    49201 aattattgat atcgtaagcg atagctggaa ctttttcagc agctgcttcg atttcagcaa
    49261 ccgaacgttt tgtcacacgt gcagcacttt ctttagacat cgcttggacg gtaatttctt
    49321 tattgtttaa agcaatcaag cggcttgatt gtaatccatg tgctaaaaat gcacctgaaa
    49381 tagcatttcc tgcaatcaat ccaataactg gatgtccagc ttgacggagt tttgcataag
    49441 cagcagcact attagccaaa gccacatgga taccgatcaa ttcttcgtta tagccgtatg
    49501 cttggcttgg tacatcgatc acaacgatga ttggccgttt cacttttttg tctttatctt
    49561 cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc ttcaactaaa ccaacttcac
    49621 cgtttcgtac acggtacatc gggtttttag gatcaggtac gattgatgtc acgatggctt
    49681 cttctatgaa tttacttgat ttaccataat acacagtact aatagaagaa gttggatttt
    49741 tcatgccagt tagtgccgca aaccaatcgt aaccaacact tgctgcagct ggttcatttc
    49801 cttctgctac ttctggtaag tcaattgatt ttgcttgatg gttagcaatt gcttcattgt
    49861 aatcgtcaat ggccattggt tttgaaagat ctaaagaatc aagtaatgat aaataaaatg
    49921 ctgagctttc actgcgatgc gtgtcttttc gttcgtccat tgctgcgatg acagcagctt
    49981 tgattgcttc tacactatca gtcactaatt catcaatgat ttttgattct acccgttgac
    50041 gagatcctaa tgtattccaa atcaagtctt tgtcactaga atcaaattct cgaacgcctg
    50101 cttcttgttc aataacttct ggtccattca aaccgactct cgcttttttt gttgcgatta
    50161 aataactcat taatgctgaa gtaatcgaca taccgccaaa tgatcctaca cgtcctggaa
    50221 tcaaaccgat gactggtaca tattgtttta gagcaatgat tacatttccg atttctgaaa
    50281 tagataacag tccgtagttt gcttcttgca aacgaacacc acctgtatct agtacaatga
    50341 ctggaaagat ctcatttcct tttttatttt cttctaacac atgttcaagt gcagcaacga
    50401 tttttgcacc ggaaacttca ccgataccgc caccttggaa ttttccttcc atagcgatta
    50461 ctactgcttg tttgccattg atcgtgccac ggctgacaac gatcccgtca tcactttcag
    50521 ggacgattcc ttgtgctggt aaacttggcg cgatgatttg atcgaatgga tcaatcaatt
    50581 cacgtccatt gtcaagtaga gcgttcactc gttctctggc gtttaattct acaaaactat
    50641 ttttcatctg aaagttcctc cattgcttgc gtcaatcgta ggtacacaac tcctggtgta
    50701 gcaccgaagt cattgatcac gatatctgct gaaattggat aacgatcaaa aaatctcgtt
    50761 aatacattgt tccatacttc gccgaagcca tcgcttcctg tacaaatttt tacagtagat
    50821 tctgtttttt ctgttggaaa catcaagatt tccaaatctc ctgaaccgac cactcctaca
    50881 tggattttct tattgatcgg ctgtttcgtt ggatatttat aagctaattg ttccatggat
    50941 ctctcccctt tttgtaaaac agagcataca cgttctgctt attcgtcttt cttcttccat
    51001 ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta tgaccaagtt ctgaatttca
    51061 ttggtggttc atacaggcca tctgaccatt cgatcaaatc atcgatggtt tgtgcagcta
    51121 ataatgaacg tttcgcttca ctacgtttga tgcctagatc ttctggtaat ttcacgatgc
    51181 cggctgcacg caagctatct agtgattctt tcgtgctagt cattccaaga ggtgtaacac
    51241 cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt ttttgctttg tacaaataag
    51301 caattccttc ttctgtcacg atatgtgtag tatcttcgct ataaatcatc actggtgcat
    51361 tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa ttcttcgaca aagactggtt
    51421 ttttatttga accatatgtt tcaaccattt gaacaactaa tttttggcct ttccctaatg
    51481 gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga atgacgacga ccgccaggat
    51541 tatgtcccat gttaggcgca ccgccaaaac cagataaacg tccttttgtt acggtagaag
    51601 agtttccttc gtaatctaat tgcaaggttg aaccgataaa catatcgatg gcatattgtc
    51661 ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc gtctttaccg acaaaaaaga
    51721 catctgggcg agcagcaata tatttttcca taccaacttc gccaccaaaa ctatggatgc
    51781 tttcaaccca acctgattcg atggctggaa tcatcgttgg atgtgggttc aatacccagt
    51841 tcttcgcgat tttccctttt aaccctagtg attcaccata agttggcagt aacaattcga
    51901 tagctgctgt attgaatcca ataccgtgat tcaaagattg tacgccgtgt tctgcgtaga
    51961 ttcctttgat tgccatcatt gccattagaa tttgcaattc tgtgatgttt tgaggatctc
    52021 gtgtgaataa agcttctaat tgataaggtt tatctgcttt cacgacaacg tctacccagt
    52081 caccagggat atctacacgt ggtaaagaat cgacaacttc gttaacttga acgatcacga
    52141 taccatcttt gaaagctgca gcttcgatga ttgttggtgt ttcttccgta ttgaaacctg
    52201 tgtacaagtt gccgtcttta tctgctttat ctgcagctac taaaacaaca tttggaatca
    52261 aatcgatgaa taagcgtcca taaagttcca aataagtatg gatatcacct aatttcattg
    52321 tgccgtcttc aatcatttgg gcgacacgca agctttgtgc accagcataa gagaaatcga
    52381 tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc tggtcgtgag atactagaca
    52441 tgatcaaatg cagatcattc actttttctg catctacttt ttcaagactt tctgaaagaa
    52501 aacttgcttg tttttgattg tcgccttcta atacaacttt gtcgccggga gcaatcagtt
    52561 tttccattac ttctacgatt ttttcagttt ctacatactt gccattgagc aagttgctga
    52621 ttttttccaa tcgctgcgct tttttttcgc gtttagaagc ccagttgcgg tttggttttt
    52681 ccataattag tcatctccat ttttgatata gaaacgtggc gtcatcaata ccatgttggt
    52741 ctagattgat cgtacggatc cctttatcta caccaaattt tgtatgaatc agctttgtca
    52801 aagtattgcc ggggggaaat tcaatcgtca gatccattcc taattctttt gccaagtcca
    52861 tcatttggtt ccactgcaca gggaaagcta ggttttccaa caaatcttta cgaatctctt
    52921 ctgcgtcctg agtggctctt ccatccgtat tttttatata caaacatcta gcatcagata
    52981 gatggacaga ctgcatatac ggcttgaatt tcttgaccgt atcattcatc aagagacagt
    53041 gagatggcac agggactttc agcagcttcg ctgtttgcgc ttggtgccgt ttggcttctg
    53101 ccaacgtaac ggataaacct tcgatactcc cagataacgt atgttgcatc ggacaatttt
    53161 catttgacag ataaactgga tggtcaggat caaatgtttt tgctacaatt tcctcagctt
    53221 cgcttctcgt cacacctact actacgccca ttgcataacc gcttggataa gcttctttca
    53281 ttaagactgc gcggtgatag actagttccg ctgcattctc aaatctcaaa gatcgacaag
    53341 caacagctgc acaaaaagca ccaatcgaat gtcctgctgc aatatcaggc tgaataccgt
    53401 ttttttccat ttcatctaaa taaaatactg cttttgtcag taatgctagc tggataaaaa
    53461 ccgtatcttg atagttttct tctttatcaa ctaaccgata ccccgtcaca ttttctaaac
    53521 gttccatgta catttcaggg atatccgcta acatccctaa cttctgactg ccttgtccag
    53581 gaaataagaa acctgtactc atgagcctcg cctcctcagc acttactttt tccactgcta
    53641 tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa gaaaaaacca taacaaaaag
    53701 gtatggatgg gatggataat tatgaaattg cttcatctgc agtattttaa aaaagtattc
    53761 gaaacaaaaa atgttaccca agcagccaaa gaattattta tttctcaacc tgccttgagc
    53821 cgtgcgatca aacatttgga aaacgaatta ggtgtccctt tgttttacca tagcggcaga
    53881 aacattgaga cgaccgttta tgcagaagaa ttttatccat atgcagtcaa gacattagac
    53941 acattagaag aaggaatcaa attattgtct tcggtcaatg agaagatcgt tacttccgtc
    54001 atattgtatt tagaggtagc ctctgtttct attccgaact tggtccgaat ttttatggaa
    54061 aagcatcctg atatccaatt aacgatcatg caacatcatt taccagatga tattaccgat
    54121 cctgtactgt acatcacttc ggagatgaag cctggcatga ccaatattcc tatcatgaaa
    54181 gaacctgttt atgtcgccat tcctaagaaa catcctcttg ctaaaaaaga acatctgcaa
    54241 ttagaagata tccaacgcac ccccatcttg atgctgtcaa agagcaacgc cttccgtcat
    54301 acactggatg cagcacttga agcgaaaagt atcgaattga cgatcagttc gacgacagat
    54361 gatcctgcga cattacgttc tattgtcaag caaggattag ctgttagctt tttcccaaaa
    54421 gtttcttggt cttacgataa aaatgatcct ttcgtcttgc gggaaatcaa agatttgccc
    54481 ttgacacgaa caatttattt atcttctagc ttcacagaag atcatccttt gatcaaaatg
    54541 attgcaaaaa cgatgcggga attttattta ggaaaataaa aaaaaaagag ctgggacaaa
    54601 attcttgtcg cagctctttt ttcgaacaaa cggtgttcca gaagtaattt cttagaaaat
    54661 aagccgaaat ttcccaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    54721 agctaaacat ttctgtcaca acctcttctc tattgtccta gacattcatt gcgttgattt
    54781 aaaacttcca tcaatcaaat ctgctagttc atctagcttt tctattttac gtagatcaat
    54841 agagatccaa tgttctttat tcatgtgata ggctggaaaa tacccctctt ttcctcgcaa
    54901 aattccgcca agttcaggat cgaccttcaa atcaagaata ttgatgatcc tatggtcact
    54961 tgcatcatca tagatttttt cttctggaac agacatcagg accccaaacc attttttgct
    55021 tttttgatgt ctgaatacgc aataatcagg aaatttttta aaaatatatt ctggagacaa
    55081 attgtatgtt tcattgatgt gatcgattag ttcttttttc gttagcatct gcttccatct
    55141 ctttcttatt gattcgcttt attcaaagat accacgtaag aatagaaaaa tgaatacaag
    55201 tagttgctaa tcaaatactt cgatattttt cttttttatc aactcttaaa tgttcaatcc
    55261 gcctgtgttg gtcttgctac catctactat taaaatgttt attattgttt gcccacctat
    55321 tttttgattt tagtggtatc atttttcaag ttcatgatga aattcttatc atcagtaaat
    55381 gctccagaaa aataaacaat gattgaaaaa tcagttgaaa cttgaccacc gtttattttc
    55441 tccgtattct tagaatagaa gggagtctga ttgatgtatt cactaatgaa aaaaatcatt
    55501 accgaaaaag atattcgacg tcatgtttct ttagtagaac aattgctgaa tcatacaaag
    55561 ataaccgtaa atgaattagc tgaaatcata gggacaaccg aacgaacgat tttttctgac
    55621 cttcaatcta ttcgctctca gctgcccgaa ggctgggaca tcatcagcga tcaagcagga
    55681 atcagtttgc aaaaccagca aaatttatta acaaatgacc tttgggagat ttttttcaaa
    55741 caatcagtca gtgtcgaact gctgaaaaat ctgcttttta caaaaaaagt tgctgtccct
    55801 gattttctag ccgaccatgg gctttcttat gggacattga aaagacatgt gactaaaatc
    55861 aatcagcgtt tagcaagtta cgacttgcag atcgatttga cgaaatatac agcttgtatt
    55921 cttggaaaag agcgtgcgat tcgtactttt tatcatcgat tgctgattcc tttcactcat
    55981 aacaactatt ttttcgagga ttattcgatc catgaatccc attattttca atttttaaga
    56041 aacctcagtc aaactgaact agcggttgaa acagaagaga ttttcggaac atgttggttc
    56101 tttatcaata cgatccgtat aaaagcgaat tgccgattag attcttctat ccataccaac
    56161 agcacactct cctccctgta tgactctgca ttgaaaaaat tgtatttaac ggaaggtatt
    56221 tatcttaaag acactgagct ttcttttgct tctttctgct ttctggaaag ttggaattac
    56281 aacaataact atggacaaga aaccgctcgc tgcctgcatc attctccatt tttagaggtc
    56341 ctcgaaacgt ttgtagagga actagcttca gagctgtctc ttgatcaact aaaaaaaaca
    56401 tctctgactg ataacttggc tctcctcata ttaaaatatc atgaatcgcc gatattgatt
    56461 gaagcattgg accgtcaata ccatcgtttt ttagaaacat atgatcagca atatgcacac
    56521 ttgtatcaac agaaagagaa tttactgaac gatctaaaaa aagaaataga aatcaatgaa
    56581 ccaaattact ttctccaact cttgtctcta ctgatccaac aaacgatttt ttctatcaaa
    56641 ccgaatttgt tcaatgtcta ttttattttc caaggagaac cttcatggaa ggcgttcttg
    56701 cagcaggaat taaaagatta cttaggaaaa cgtgtctgct tcttacctat cgaactaacc
    56761 gatctttcca ctatttcttt tgaaaaaaca gatatcttga tttccaattt ccctctagat
    56821 tctctagata tccctattgt atacatttct tctattccaa cgaaaaatga gctcaatcgt
    56881 ttgactgaac tcactttcaa aactttttta tagtaacggc tagcgactgt attctctagc
    56941 tgttttttct tttttgcgaa acagtttgtt catgatccct ttttctattt ctacaactaa
    57001 gaatagaaga atcacatttg ctccaatcaa taactgttgt gtcatcgtta aacttactgt
    57061 accgatcagc tgctggccga ctggtagaaa aataaccatc ccttgcaata aagcaaggat
    57121 acctaatgaa agaaaaagcg ctttgttttg caaaagacct tggttcaatg acggcttgga
    57181 caactctctg caattgatca tgtaaacagc ttgagctaag acgatatttt gcagaagaat
    57241 ggtttgctgt aagctttgcc cttcaaactg catagccaat aaataggcag gaaccatgat
    57301 cagtagggag acatagatga tccgaaacaa atgataacca gataggattc cttgctttgg
    57361 atctcttggt gggcgcttca tgatttctgg atctggcgac tcgaacccta gtgcataaga
    57421 taaagtgatt gtcgttacca tattgaccca taaaatctgt actggggaca aaggtagcgg
    57481 gcgattcagc ataagtgccc aaatcaagat caatccttga gctaatgcag ttggcaagaa
    57541 gaaattgatg gttttttgca aattatccaa aatccgccgg ccctctttta cggcttttgc
    57601 aatcgtatgg aaattatcat ctcctaaaac catatcagct gcctgtttgc ttacttcact
    57661 acctttgatt cccatagcaa ttcctacatc ggatcttttc aaagcaggtg catcatttac
    57721 gccgtctccg gtcattccga cgatttctcc atttttctgc aaagctttaa caattcgcag
    57781 tttatgttct ggtgtcgttc gagcaaagac atcgactatt ggcacttgct gaatcaactc
    57841 ttcatcagac ataagatcca aatcgatccc ttctaaaact ttggcagtat gttttaagcc
    57901 aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc cctgtaatca ttttcactga
    57961 gatccctgct tgttggcatt cctttacagc tttgactgca ctttcttttg gcggatcgat
    58021 caatccggcg atccctgcaa aagttaattg ggttaaagtc tcatgcgtga gttcctgttt
    58081 cgaatctact tttttataag caaatcctaa aacacgctga ccttttctcg caaattctgc
    58141 agctgtctga ctccatgctt cttgttctgc agaagaaaga gttgatttag caaataatac
    58201 ttctggtgca cctttgacgt aaatcaccgc tcctttttcc tccgcatgca tcgttgccat
    58261 atatttgtat tcagaactga acggaatctt tgcttctatt tttttcaaag gtagattggc
    58321 tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg ccacttaaat tttctgtttt
    58381 ctgttcttct tttgttttta attcctgaca gtttttcata atggacaaca cgcaggcttc
    58441 ttcttttgca gctacatcca caactgtcat ttcatttttt gtcaatgttc ctgtcttatc
    58501 tgaacagatc actgtcatag aacctaatgt ttctaccgaa ggaagtcctt tgatgatcgc
    58561 cttttctttt gccatttcat gtactccaac ggatagaatc atggtcaaaa cagctggcaa
    58621 tccttcgggt accattgaca ccaccaaagc aatcgtagcc gaaagaagaa tatgccaatc
    58681 cataccatat cgaaatgtcg taaaaataac taagaatagg ctcaaacaaa gaatgccttg
    58741 aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga ggcgtcgttt gttgttcgac
    58801 cgattgcaat gcttgattga ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac
    58861 accgactgca gaaccagatt gtacaagcgt tcctgaaaaa cccatatttt tctggtcgcc
    58921 gatcgattct tcttctgtca aaacggcatg acttttttca gatgcttctg attctcccgt
    58981 caaaatcgat tcttccacta ataaatcatg tgcttcgatt aatcggatat ctgctggaac
    59041 gacatctccg gcacgtaaga caatgacatc tccttttacc agttctttcg cagggatttt
    59101 ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc atcagctgct tcaaaccatt
    59161 caatgattct tccgcttttc tttcttgcca gtaactaacg agtccattga cgatcactac
    59221 gaataaaata atacttcctt caatatattc actagaagca aatttcaaaa aagaggcaat
    59281 gatcaacacg atcatcagca gatcagtaaa atgctttgct atttttttcc atcgttttgt
    59341 ctgctgcctt gcttttattt cattatatcc ttgatcagtc aggcgtttct gtcgttcttc
    59401 ttgcgacaag ccttctgttg atgtttgata gtttttcaat acatcttcta ccgtcaatct
    59461 gtaccagttc atcctatccc ctcctgttta attgatactg ttattatacg cagggaagtt
    59521 ttttcaggcg tatcgattat ttcagggggg tctgaaataa ttcgttttgc aaaatgtatc
    59581 tgggcctaac tgatatttct attgcttatt cttttatata caaaatttaa gaatacaaac
    59641 cagataacca ataaaaaagg aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca
    59701 attgaagtaa aaaacaccaa caaagcaaga tctttatatc tttgtgtagt gttccgtttc
    59761 ttttctttaa taaattgaac atgtgcacac aagatcgaag accacacaaa tagaaaacaa
    59821 ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt
    59881 agacaaagac cggcaaaaaa taacattcct gtaattaaaa taccgtgtaa aggtacgcct
    59941 ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt cttttgctgt ttgatataaa
    60001 atcctgctag tactgtagac tgcactattt gctgaggaca aagaagcaac caaaatcatt
    60061 agattgacaa ttgctgctcc gccttggcaa tggatcactt gcagcatagt cactaaagga
    60121 ctttctttta aatctaaaca atgccatgga acaacaaata aaatcgacaa aatcgttcct
    60181 atatagaaaa agagaatacg ccaaggcaga cagttgattg cttttttcaa attcttttgc
    60241 ggatcacttg tctcgccaac ggttaagccg atcatttcaa ttccagcaaa actgaataaa
    60301 accataggaa atgagactac cattccagca aagccaaacg gtagtaattc atcgatctgt
    60361 ccaaaatttt ctttgattcc gatagactgg gtatgaaaat ctgagacact caatcctata
    60421 cccaataaga taaaaaggaa aatccccaag atcttgatga ttgagaaaaa agattccaat
    60481 tcaccaaacc acttgactgc aacaaggttg actgccatta agatcattag tgcgctacct
    60541 ccgcttatca aagctggtac atgtacaaac cagaaatgga tatacaatcc tattgctgtt
    60601 aattccgcaa gtccagctcc aagccagcaa agccaataag tccaattgac aaaaaatgcc
    60661 cactttttgc ctaaataata gtcaacgaaa tcactgatag agttaaatgt actatttgct
    60721 aaaaataaat cacccatggc ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa
    60781 acgagaataa cagctggccc tgctgatgca attgcctttg ctgaacctaa aaataatcct
    60841 gttccaattg cgccacctaa tgcgattaac tggatatttc gattagttag agtccgtttc
    60901 attgccggtt tttccatcaa gttcaccttt ttctttttca agcagcatgg cgacttcctg
    60961 ttcccaagaa ggtttcttta ttttttcaat acagagtggt actacaaaca aaatgacact
    61021 gatggctgtg actgcaatta gattgatcca tgttccagct ttcatcgtca gtccgacaga
    61081 aaatccgatt gccgcaaata aaacaataac gacagtccaa actccaagat tgctcttttt
    61141 ctcttttttt ccaattctaa atggtcgatt caactttgca tgtgtataac gcaatctcaa
    61201 aactgcgatc cccatgatga catagacgat acagtagata acagtagttg aatttacgag
    61261 catcaaaaat gcttgattga cgccaggaat cagcaaataa aaaagtgcaa agattgagat
    61321 aatgacagac tgagtcaaaa taacggattt ggatacgtca aattgattcg tcttccaaaa
    61381 attgaatttt ggtggatacg caccttttct agcactttct gtcactgtct ttgctggtcc
    61441 tgaggcccaa gcagataatt ggactgctac accaatgaag acaagtaaac taaacagatt
    61501 cacaataaga tgtggcaaac caagaatttg gcaataaatc gaaatagatt gcgcgatatt
    61561 attcaattcc atttgtcctt ttggtacgac atttgctaca actaaagcat tcaaaatatt
    61621 gacgaaaaat ataaaaatta acgcggcaaa tactccgcga atatacgttt taactggatt
    61681 ttccaatcga gtgatataca cagaggacat ttcgatacca gtaaaaataa acatgatcgg
    61741 cgcaaaataa acaaatgagg atgcagtcgt agtatctggt attaatttgt cccatgaaaa
    61801 actgcctaat gttccggttg gtacgatccc aactttgatc cacgcagcaa accctaataa
    61861 gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca ccgacttttc cgccgatttt
    61921 tgccatatca aatttcaaat tcaaaaaagt gatgatccaa taaacgacta aaatgacaac
    61981 taatgtaaat ttgctgttca aaccaagcgg cacattatcg ataatattac caaacaacgg
    62041 agcgagtgct gaagcgacca tcaccatccc aggaaacatt tggacccata acagccatga
    62101 gacaacgaac ccccattttt ttcctagagc attcgatacc caaagttctg gaccaccttt
    62161 ttggggaaac attccagcaa attcaccaga gatcaatgag attggaaaag cgtacaataa
    62221 tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt gctgctacat ctggaatatt
    62281 tcgtacactt gctacaaggg cgcaggtcat tccgataaag gtcatggtcg tgatttgctt
    62341 tgatgaacca gtatctttca tctttattcc ttctttcaat agcgacatat gtttttgttt
    62401 tcttttattt ttgttcaaga acagaaatga caaggtttca tcgattcagt acaaaaccca
    62461 gtatcaccga aacatccatt gaacgatctt agtgaactgg gtttgataca tgaaacgaac
    62521 gatccaactt atagaacgtt ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac
    62581 agggggaaat acaattgttt tgatgaagca aataattgtc tgttctccat atgtctcttg
    62641 ttattctttc acacaaaaat cagttctatt ggatgatttc gtttagtttc ttttccattg
    62701 ctttggcgat tttttttgta taaaaattga aaattcgatc atcattcaaa taaggagtca
    62761 tgatagccgc acgtagcaga gtgattttct gttctttttt ccattcttct ttcgttagac
    62821 ccattctttc aacaaaacga atcggagaat caccgtaatc ttcttgtgta aatgtagtat
    62881 gagaagtaat aaaccgttca ccataaacat cgccatccat ataagaagac cgatcgaaca
    62941 ttttttcatt taattcattg atagcgttca aatctgtaca gccttgttct ttgaataccc
    63001 aattgaccat attaaaatca ggatgattca atggatagac ttcgattgtt tttcctttaa
    63061 cagtaaaagt cagatgatcg agaaattctc tgaatctttg agctgcctcg attgaagccc
    63121 caatcaattg accatatccg gtcacattca atggcaatac acgatgtgct gtccagactg
    63181 cggcggcggt tgctccagct tttgatcctt ccaaaatata agctcctaac atatctggag
    63241 cctttactga tttttcaaac acatatggcg cgaaataaga aatgatattt ctcatatttt
    63301 tatgtttgat tacgattcct ccagcagcat aagggacata gcccatctta tgaggatcta
    63361 tcgtgacaga atccgcttct gaaatagctc tgaacccttc atatacctct ttgtcaatcg
    63421 ttacgcagtg gtggaaaaca tgatgttctt caaagaattc agctaaggaa gcatacggca
    63481 caaattctcc tgcttcgttt agaaacagtg aacgcgcata gccgccatat gcggcatcaa
    63541 catgtaaata gaaatagatc ccttcatctt ttaacctttc tcttaattgg acgattttat
    63601 ctacactgtc tacttgacct tcttctgtcg ttcctacgac agcaaccaca cctagaattg
    63661 ggattttttg tccagctaat tcacgaattg ttttttccaa agcattaata tccattcgat
    63721 aatcctcttg aactgggata gcaaccatct gatctaagcc gactccgcaa atatctaacg
    63781 ccttcatcca agaatagtgt tttgtttgag ggaccagcca tttccctaat ctttggatat
    63841 tttttccgct acgggaagaa gctgctttga cctcatccat ttcttcgtcc gtaaagcttt
    63901 cggtcatttc caaaatttct tcaacagata aattcaaaag ttcccactca gacatttttt
    63961 tcactttttc aggcagcact tccttgacag ctaaaggaat cgatttgatg caacgggcgt
    64021 accataatcc ttcaagatta gcaatagaac catctgctgc aatgtgtccc catccgtctg
    64081 tcatattgaa taatgaagca aaatcctgtc ctacttctgc ttccatttgc gaagttgcca
    64141 tagaagattc caatgcaaca ttatttgggt tccacagcat cgcatagttg taagctaata
    64201 aagcaggcat caatgtttca gcattcattt gtccccagta acggcctgct gaatgccatg
    64261 ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa gacctctatc atatggtcgc
    64321 gggtggctag ataatctgga gtgagcttat ctccttcgct aattgcaggc atatctgaag
    64381 gaatataatt ttctctccat ccaagatgtt catctaccat cttgttcaac aacattttat
    64441 aaactggtcc attttcagct ttgtcgccta taaagacggc cttgatatcc atatctttca
    64501 tttttcttcc ctccatattt tgttggtcaa tgtcttgcag atacatgtgt ttatctgtaa
    64561 cacctcttca ctatagcgcc aaaaatgaga attttttttt tataacatca aatccacgta
    64621 gatttttaca aacaaaaata aataaagtcg gatttttctg ataattcaac gcaaaaaagc
    64681 gaacaatttt tcaataaatc agtcttctaa aatagataac ctaaaaaaag
//
LOCUS       DACCWV010000004        50883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCWV010000004 DACCWV010000000
VERSION     DACCWV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.004"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            147..518
                     /locus_tag="IVW84_000228"
     CDS             147..518
                     /locus_tag="IVW84_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7916458.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(792..1484)
                     /locus_tag="IVW84_000229"
     CDS             complement(792..1484)
                     /locus_tag="IVW84_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7916459.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1487..2272)
                     /locus_tag="IVW84_000230"
     CDS             complement(1487..2272)
                     /locus_tag="IVW84_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7916460.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2509..3909
                     /gene="lacG"
                     /locus_tag="IVW84_000231"
     CDS             2509..3909
                     /gene="lacG"
                     /locus_tag="IVW84_000231"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7916461.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3934..4251
                     /locus_tag="IVW84_000232"
     CDS             3934..4251
                     /locus_tag="IVW84_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7916462.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4264..5937
                     /locus_tag="IVW84_000233"
     CDS             4264..5937
                     /locus_tag="IVW84_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7916463.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5951..7582
                     /locus_tag="IVW84_000234"
     CDS             5951..7582
                     /locus_tag="IVW84_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7916464.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8073..9638)
                     /gene="guaA"
                     /locus_tag="IVW84_000235"
     CDS             complement(8073..9638)
                     /gene="guaA"
                     /locus_tag="IVW84_000235"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7916465.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9697..10620)
                     /locus_tag="IVW84_000236"
     CDS             complement(9697..10620)
                     /locus_tag="IVW84_000236"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7916466.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10816..11421
                     /locus_tag="IVW84_000237"
     CDS             10816..11421
                     /locus_tag="IVW84_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7916467.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11729..12745
                     /locus_tag="IVW84_000238"
     CDS             11729..12745
                     /locus_tag="IVW84_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7916468.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12886..14187
                     /locus_tag="IVW84_000239"
     CDS             12886..14187
                     /locus_tag="IVW84_000239"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7916469.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14233..14895
                     /gene="deoC"
                     /locus_tag="IVW84_000240"
     CDS             14233..14895
                     /gene="deoC"
                     /locus_tag="IVW84_000240"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7916470.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14916..15314
                     /locus_tag="IVW84_000241"
     CDS             14916..15314
                     /locus_tag="IVW84_000241"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7916471.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15380..16462
                     /locus_tag="IVW84_000242"
     CDS             15380..16462
                     /locus_tag="IVW84_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7916472.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16674..18236
                     /locus_tag="IVW84_000243"
     CDS             16674..18236
                     /locus_tag="IVW84_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916473.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18229..19362
                     /locus_tag="IVW84_000244"
     CDS             18229..19362
                     /locus_tag="IVW84_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916474.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19362..20318
                     /locus_tag="IVW84_000245"
     CDS             19362..20318
                     /locus_tag="IVW84_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916475.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20417..21583
                     /gene="deoB"
                     /locus_tag="IVW84_000246"
     CDS             20417..21583
                     /gene="deoB"
                     /locus_tag="IVW84_000246"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7916476.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21607..22425
                     /locus_tag="IVW84_000247"
     CDS             21607..22425
                     /locus_tag="IVW84_000247"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7916477.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22442..23149
                     /gene="deoD"
                     /locus_tag="IVW84_000248"
     CDS             22442..23149
                     /gene="deoD"
                     /locus_tag="IVW84_000248"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7916478.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23447..24133)
                     /gene="gpmA"
                     /locus_tag="IVW84_000249"
     CDS             complement(23447..24133)
                     /gene="gpmA"
                     /locus_tag="IVW84_000249"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7916479.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24305..24886)
                     /locus_tag="IVW84_000250"
     CDS             complement(24305..24886)
                     /locus_tag="IVW84_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7916480.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25019..25702)
                     /gene="rpiA"
                     /locus_tag="IVW84_000251"
     CDS             complement(25019..25702)
                     /gene="rpiA"
                     /locus_tag="IVW84_000251"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7916481.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26021..26434
                     /gene="rpsL"
                     /locus_tag="IVW84_000252"
     CDS             26021..26434
                     /gene="rpsL"
                     /locus_tag="IVW84_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7916482.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26518..26988
                     /gene="rpsG"
                     /locus_tag="IVW84_000253"
     CDS             26518..26988
                     /gene="rpsG"
                     /locus_tag="IVW84_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7916483.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27082..29169
                     /gene="fusA"
                     /locus_tag="IVW84_000254"
     CDS             27082..29169
                     /gene="fusA"
                     /locus_tag="IVW84_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7916484.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29341..30528
                     /gene="tuf"
                     /locus_tag="IVW84_000255"
     CDS             29341..30528
                     /gene="tuf"
                     /locus_tag="IVW84_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7916485.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30935..31243
                     /gene="rpsJ"
                     /locus_tag="IVW84_000256"
     CDS             30935..31243
                     /gene="rpsJ"
                     /locus_tag="IVW84_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7916486.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31274..31903
                     /gene="rplC"
                     /locus_tag="IVW84_000257"
     CDS             31274..31903
                     /gene="rplC"
                     /locus_tag="IVW84_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7916487.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31931..32554
                     /gene="rplD"
                     /locus_tag="IVW84_000258"
     CDS             31931..32554
                     /gene="rplD"
                     /locus_tag="IVW84_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7916488.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32554..32844
                     /gene="rplW"
                     /locus_tag="IVW84_000259"
     CDS             32554..32844
                     /gene="rplW"
                     /locus_tag="IVW84_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7916489.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32883..33716
                     /gene="rplB"
                     /locus_tag="IVW84_000260"
     CDS             32883..33716
                     /gene="rplB"
                     /locus_tag="IVW84_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7916490.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33759..34037
                     /gene="rpsS"
                     /locus_tag="IVW84_000261"
     CDS             33759..34037
                     /gene="rpsS"
                     /locus_tag="IVW84_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7916491.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34060..34407
                     /gene="rplV"
                     /locus_tag="IVW84_000262"
     CDS             34060..34407
                     /gene="rplV"
                     /locus_tag="IVW84_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7916492.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34421..35077
                     /gene="rpsC"
                     /locus_tag="IVW84_000263"
     CDS             34421..35077
                     /gene="rpsC"
                     /locus_tag="IVW84_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7916493.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35080..35514
                     /gene="rplP"
                     /locus_tag="IVW84_000264"
     CDS             35080..35514
                     /gene="rplP"
                     /locus_tag="IVW84_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7916494.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35504..35692
                     /gene="rpmC"
                     /locus_tag="IVW84_000265"
     CDS             35504..35692
                     /gene="rpmC"
                     /locus_tag="IVW84_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7916495.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35717..35983
                     /gene="rpsQ"
                     /locus_tag="IVW84_000266"
     CDS             35717..35983
                     /gene="rpsQ"
                     /locus_tag="IVW84_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7916496.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36041..36409
                     /gene="rplN"
                     /locus_tag="IVW84_000267"
     CDS             36041..36409
                     /gene="rplN"
                     /locus_tag="IVW84_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7916497.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36446..36754
                     /gene="rplX"
                     /locus_tag="IVW84_000268"
     CDS             36446..36754
                     /gene="rplX"
                     /locus_tag="IVW84_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7916498.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36782..37321
                     /gene="rplE"
                     /locus_tag="IVW84_000269"
     CDS             36782..37321
                     /gene="rplE"
                     /locus_tag="IVW84_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7916499.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37340..37525
                     /locus_tag="IVW84_000270"
     CDS             37340..37525
                     /locus_tag="IVW84_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7916500.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37561..37959
                     /gene="rpsH"
                     /locus_tag="IVW84_000271"
     CDS             37561..37959
                     /gene="rpsH"
                     /locus_tag="IVW84_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7916501.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37991..38527
                     /gene="rplF"
                     /locus_tag="IVW84_000272"
     CDS             37991..38527
                     /gene="rplF"
                     /locus_tag="IVW84_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7916502.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38688..39044
                     /gene="rplR"
                     /locus_tag="IVW84_000273"
     CDS             38688..39044
                     /gene="rplR"
                     /locus_tag="IVW84_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7916503.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39065..39565
                     /gene="rpsE"
                     /locus_tag="IVW84_000274"
     CDS             39065..39565
                     /gene="rpsE"
                     /locus_tag="IVW84_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7916504.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39580..39759
                     /gene="rpmD"
                     /locus_tag="IVW84_000275"
     CDS             39580..39759
                     /gene="rpmD"
                     /locus_tag="IVW84_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7916505.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39803..40243
                     /gene="rplO"
                     /locus_tag="IVW84_000276"
     CDS             39803..40243
                     /gene="rplO"
                     /locus_tag="IVW84_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7916506.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40243..41538
                     /gene="secY"
                     /locus_tag="IVW84_000277"
     CDS             40243..41538
                     /gene="secY"
                     /locus_tag="IVW84_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7916507.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41600..42247
                     /locus_tag="IVW84_000278"
     CDS             41600..42247
                     /locus_tag="IVW84_000278"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7916508.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42438..42656
                     /gene="infA"
                     /locus_tag="IVW84_000279"
     CDS             42438..42656
                     /gene="infA"
                     /locus_tag="IVW84_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7916509.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42689..42805
                     /gene="rpmJ"
                     /locus_tag="IVW84_000280"
     CDS             42689..42805
                     /gene="rpmJ"
                     /locus_tag="IVW84_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7916510.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42823..43188
                     /gene="rpsM"
                     /locus_tag="IVW84_000281"
     CDS             42823..43188
                     /gene="rpsM"
                     /locus_tag="IVW84_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7916511.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43216..43605
                     /gene="rpsK"
                     /locus_tag="IVW84_000282"
     CDS             43216..43605
                     /gene="rpsK"
                     /locus_tag="IVW84_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7916512.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43685..44623
                     /locus_tag="IVW84_000283"
     CDS             43685..44623
                     /locus_tag="IVW84_000283"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7916513.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44651..45031
                     /gene="rplQ"
                     /locus_tag="IVW84_000284"
     CDS             44651..45031
                     /gene="rplQ"
                     /locus_tag="IVW84_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7916514.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45303..46142
                     /locus_tag="IVW84_000285"
     CDS             45303..46142
                     /locus_tag="IVW84_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7916515.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46118..46987
                     /locus_tag="IVW84_000286"
     CDS             46118..46987
                     /locus_tag="IVW84_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7916516.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46977..47774
                     /locus_tag="IVW84_000287"
     CDS             46977..47774
                     /locus_tag="IVW84_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7916517.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47977..48723
                     /gene="truA"
                     /locus_tag="IVW84_000288"
     CDS             47977..48723
                     /gene="truA"
                     /locus_tag="IVW84_000288"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7916518.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49388..49625
                     /locus_tag="IVW84_000289"
                     /pseudo
     CDS             49388..49625
                     /locus_tag="IVW84_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49769..50545)
                     /locus_tag="IVW84_000290"
     CDS             complement(49769..50545)
                     /locus_tag="IVW84_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7916519.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50668..>50883
                     /locus_tag="IVW84_000291"
     CDS             50668..>50883
                     /locus_tag="IVW84_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7916520.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIIT"
BASE COUNT        16135 a         9266 c        11052 g        14430 t
ORIGIN      
        1 atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt actgataatg
       61 aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt
      121 actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag
      181 tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg
      241 ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg
      301 ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag
      361 atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga
      421 aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa
      481 ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa
      541 aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa
      601 acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga
      661 aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg
      721 tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt
      781 agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta
      841 aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta
      901 ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct
      961 gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct
     1021 ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg
     1081 tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg
     1141 acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct
     1201 tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag
     1261 ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa
     1321 tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat
     1381 aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag
     1441 tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag
     1501 tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa
     1561 cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca
     1621 cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat
     1681 tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt
     1741 tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt
     1801 tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa
     1861 tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc
     1921 gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga
     1981 aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt
     2041 gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac
     2101 tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc
     2161 tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc
     2221 cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca
     2281 cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa
     2341 agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt
     2401 ttttatgatt gtttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa
     2461 tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta
     2521 ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag
     2581 gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt
     2641 cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt
     2701 tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct
     2761 gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa
     2821 agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac
     2881 catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt
     2941 tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca
     3001 accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc
     3061 gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa
     3121 aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca
     3181 gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta
     3241 ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct tgatgagata
     3301 tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa
     3361 gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct
     3421 tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat
     3481 aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac
     3541 tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca
     3601 cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat
     3661 ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc
     3721 aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt
     3781 ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc
     3841 cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg
     3901 attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt
     3961 ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa
     4021 gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat
     4081 ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag
     4141 atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg
     4201 agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac
     4261 gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca
     4321 cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta
     4381 ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct
     4441 gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta
     4501 ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa
     4561 aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta
     4621 agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa gggactactg
     4681 gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat
     4741 attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt
     4801 tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc
     4861 ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct
     4921 gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt
     4981 gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa
     5041 accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta
     5101 gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc
     5161 ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta
     5221 agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt
     5281 tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat
     5341 gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt
     5401 ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc
     5461 gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa
     5521 gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag
     5581 gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg
     5641 gtactttgtg ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca
     5701 tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg
     5761 ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc
     5821 aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc
     5881 ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg
     5941 aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt
     6001 aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg
     6061 agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga
     6121 tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg
     6181 tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct
     6241 ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga
     6301 ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca
     6361 aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc
     6421 ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag
     6481 cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt
     6541 ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc
     6601 agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga
     6661 aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca
     6721 cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga
     6781 agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa
     6841 tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc
     6901 agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg
     6961 cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg
     7021 tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt
     7081 tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat
     7141 aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg
     7201 gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt
     7261 ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca
     7321 aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg
     7381 agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc
     7441 ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg
     7501 tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt
     7561 tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt
     7621 cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat
     7681 aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac
     7741 actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa
     7801 aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg
     7861 aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg
     7921 agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta
     7981 gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta
     8041 tagggattcc ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt
     8101 ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca
     8161 gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt
     8221 gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg
     8281 acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca
     8341 gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca
     8401 gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg
     8461 attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg
     8521 tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga
     8581 gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct
     8641 tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca
     8701 tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac
     8761 ttgtctaaga agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct
     8821 agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata
     8881 gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg
     8941 ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact
     9001 tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca
     9061 aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata
     9121 ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg
     9181 actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt
     9241 tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct
     9301 ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat
     9361 tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc
     9421 ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga
     9481 ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca
     9541 agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata
     9601 atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta
     9661 aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga
     9721 atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa
     9781 gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa
     9841 ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc
     9901 cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc
     9961 atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa
    10021 ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt
    10081 gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc
    10141 agattttaca cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc
    10201 tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt
    10261 cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct
    10321 ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac
    10381 ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat
    10441 ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat
    10501 gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt
    10561 acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat
    10621 attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc
    10681 caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag
    10741 tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg
    10801 agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat
    10861 tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt
    10921 gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg
    10981 gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg
    11041 ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg
    11101 gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa
    11161 tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg
    11221 atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag
    11281 aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag
    11341 aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc
    11401 atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg
    11461 ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag
    11521 aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat
    11581 aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat
    11641 caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt acactgatag
    11701 ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat
    11761 ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag
    11821 tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta
    11881 tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt
    11941 cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg
    12001 ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca
    12061 agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga
    12121 gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg
    12181 attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc
    12241 tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg
    12301 atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct
    12361 gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc
    12421 atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat
    12481 ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc
    12541 agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt
    12601 gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga
    12661 atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag
    12721 cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa
    12781 ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc
    12841 taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat
    12901 ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc
    12961 acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt
    13021 ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct
    13081 ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt
    13141 ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca
    13201 gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa
    13261 gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa
    13321 agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat
    13381 gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg
    13441 agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt
    13501 gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg
    13561 aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa
    13621 gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca
    13681 gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa
    13741 gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg
    13801 ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat
    13861 ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt
    13921 gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt
    13981 aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt
    14041 gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact
    14101 tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg
    14161 ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa
    14221 aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca
    14281 tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc
    14341 tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc
    14401 gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa
    14461 acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct
    14521 ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca
    14581 aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc
    14641 atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt
    14701 ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca
    14761 gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc
    14821 gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact
    14881 ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga
    14941 tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag
    15001 gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat
    15061 catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag
    15121 aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg
    15181 gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag
    15241 gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg
    15301 aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat
    15361 tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact
    15421 agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg
    15481 caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga
    15541 ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact
    15601 ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc
    15661 aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg gttataaact
    15721 aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga
    15781 tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc
    15841 ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg
    15901 cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc
    15961 aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc
    16021 tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat
    16081 cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga
    16141 atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga
    16201 atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg
    16261 aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca
    16321 cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga
    16381 agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc
    16441 agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa
    16501 ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg
    16561 gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt
    16621 gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc
    16681 aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa
    16741 atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg
    16801 gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga
    16861 aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag
    16921 ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca
    16981 tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag
    17041 agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg
    17101 atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag
    17161 atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa
    17221 tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt
    17281 tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg
    17341 gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa
    17401 cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg
    17461 aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg
    17521 ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga
    17581 tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag
    17641 acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag
    17701 accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa
    17761 cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac
    17821 atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa
    17881 aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc
    17941 ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta
    18001 ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg
    18061 aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg
    18121 tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct
    18181 ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc
    18241 agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta
    18301 ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta
    18361 gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg
    18421 atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt
    18481 tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat
    18541 ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg
    18601 gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca
    18661 atcatgatga actatatctt attatacgta agcacatttg ctttacatga agtgatgcct
    18721 gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg
    18781 ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca
    18841 atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc
    18901 cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg
    18961 atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga
    19021 ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga
    19081 atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg
    19141 ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag
    19201 atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt
    19261 ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa
    19321 tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa
    19381 ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta acgtcactag
    19441 gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca
    19501 tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga
    19561 caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg
    19621 cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga
    19681 tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata
    19741 acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg
    19801 gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc
    19861 tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa
    19921 atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac
    19981 tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta
    20041 acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg
    20101 gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct
    20161 tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa
    20221 ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc
    20281 caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt
    20341 gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa
    20401 gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat
    20461 tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat
    20521 tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg
    20581 tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga
    20641 agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa
    20701 aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga
    20761 attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga
    20821 ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt
    20881 ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca
    20941 attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc
    21001 gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa
    21061 tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt
    21121 tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag
    21181 caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag
    21241 ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta
    21301 tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga
    21361 agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga
    21421 tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc
    21481 attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc
    21541 ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga
    21601 aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa
    21661 ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat
    21721 gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca
    21781 gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc
    21841 atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta
    21901 cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc
    21961 aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt
    22021 gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt
    22081 caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg
    22141 gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag
    22201 atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc
    22261 atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca
    22321 gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa
    22381 caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa
    22441 aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg
    22501 agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa
    22561 tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca
    22621 aggaacagga atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta
    22681 tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt
    22741 tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt
    22801 ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat
    22861 agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta
    22921 caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat
    22981 ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat
    23041 gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac
    23101 gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga
    23161 aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt
    23221 gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg
    23281 tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt
    23341 tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa
    23401 agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt
    23461 ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt
    23521 caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg
    23581 agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat
    23641 cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat
    23701 cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt
    23761 ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt
    23821 atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta
    23881 agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac
    23941 atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt
    24001 ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat
    24061 cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa
    24121 ctaattttgg catgtttagc tctcctttta agataggttt atatttctga aattttcagt
    24181 tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta
    24241 tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta
    24301 ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg
    24361 ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat
    24421 agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc
    24481 cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca
    24541 tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc
    24601 cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta
    24661 attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt
    24721 gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat
    24781 atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta
    24841 aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct
    24901 agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga
    24961 tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt
    25021 atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag
    25081 aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag
    25141 ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat
    25201 ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt
    25261 caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact
    25321 tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg
    25381 tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag
    25441 cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct
    25501 ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg
    25561 tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg
    25621 ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc
    25681 cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt
    25741 tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa
    25801 gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac
    25861 aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat
    25921 ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt
    25981 gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt
    26041 agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg
    26101 atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc
    26161 aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg
    26221 tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt
    26281 acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg
    26341 ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg
    26401 ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt
    26461 gcttctacta tatagaagta attactatga acaatatttc ggaaggagga gttacggatg
    26521 ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa
    26581 ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac
    26641 attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg
    26701 tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg
    26761 ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt
    26821 cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca
    26881 aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact
    26941 cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt
    27001 ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta
    27061 aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc
    27121 ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat
    27181 actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg
    27241 gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag
    27301 ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa
    27361 cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa
    27421 cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt
    27481 gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac
    27541 cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca
    27601 ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag
    27661 atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa
    27721 ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa
    27781 gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc
    27841 tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct
    27901 gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa
    27961 actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt
    28021 aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt
    28081 ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt
    28141 atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc
    28201 gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt
    28261 ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct
    28321 aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat
    28381 cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt
    28441 gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac
    28501 gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt
    28561 aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca
    28621 ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct
    28681 cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta
    28741 gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc
    28801 gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag
    28861 aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat
    28921 tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa
    28981 gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt
    29041 tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac
    29101 cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa
    29161 gctgaataat ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt
    29221 tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt
    29281 acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa
    29341 atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac
    29401 gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac
    29461 ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga
    29521 atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt
    29581 gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac
    29641 ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc
    29701 ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg
    29761 gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa
    29821 tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa
    29881 ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc
    29941 ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca
    30001 attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt
    30061 gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt
    30121 gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta
    30181 cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc
    30241 acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt
    30301 catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
    30361 ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg
    30421 gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa
    30481 ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt
    30541 ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt
    30601 gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga
    30661 agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt
    30721 ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt
    30781 tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt
    30841 tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg
    30901 tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg
    30961 tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc
    31021 aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta
    31081 cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac
    31141 acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa
    31201 gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa
    31261 tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt
    31321 cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact
    31381 acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat
    31441 gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc
    31501 taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga
    31561 aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg
    31621 taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg
    31681 ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa
    31741 aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt
    31801 tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa
    31861 aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag
    31921 gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat
    31981 cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat
    32041 cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt
    32101 tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc
    32161 tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta
    32221 cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa
    32281 agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa
    32341 agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga
    32401 aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac
    32461 ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac
    32521 tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc
    32581 ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg
    32641 gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa
    32701 gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca
    32761 ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc
    32821 caattattcg aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa
    32881 acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg
    32941 attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc
    33001 atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac
    33061 gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa
    33121 cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag
    33181 tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc
    33241 cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg
    33301 ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta
    33361 caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg
    33421 aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac
    33481 acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg
    33541 tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc
    33601 ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc
    33661 gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac
    33721 tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag
    33781 gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga
    33841 aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata
    33901 ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag
    33961 gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata
    34021 agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca
    34081 gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac
    34141 cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa
    34201 tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt
    34261 gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg
    34321 aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat
    34381 atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc
    34441 aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga
    34501 gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc
    34561 tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat
    34621 tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag
    34681 aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa
    34741 accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt
    34801 tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg
    34861 gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata
    34921 ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga
    34981 agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct
    35041 tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta
    35101 aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta
    35161 gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc
    35221 gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa
    35281 attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa
    35341 ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca
    35401 ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg
    35461 aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat
    35521 cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt
    35581 taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt
    35641 acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa
    35701 ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt
    35761 ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc
    35821 tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac
    35881 agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg
    35941 tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct
    36001 atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg
    36061 tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg
    36121 atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac
    36181 gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc
    36241 aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat
    36301 tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg
    36361 tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac
    36421 ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat
    36481 cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa
    36541 agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc
    36601 tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga
    36661 ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt
    36721 ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg
    36781 aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt
    36841 taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt
    36901 tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat
    36961 cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg
    37021 tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt
    37081 agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa
    37141 agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga
    37201 agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc
    37261 aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata
    37321 attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc
    37381 aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta
    37441 tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc
    37501 cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca
    37561 atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt
    37621 aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg
    37681 aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc
    37741 cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc
    37801 tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt
    37861 ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa
    37921 aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag
    37981 gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt
    38041 tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt
    38101 ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga
    38161 cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt
    38221 aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca
    38281 attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc
    38341 accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa
    38401 caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta
    38461 taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg
    38521 taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga
    38581 agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta
    38641 tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac
    38701 cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta
    38761 ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta
    38821 ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag
    38881 gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg
    38941 aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc
    39001 aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc
    39061 attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa
    39121 ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt
    39181 cggtgacaaa aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc
    39241 aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc
    39301 aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc
    39361 tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg
    39421 ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc
    39481 aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa
    39541 atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact
    39601 ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt
    39661 cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc
    39721 aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga
    39781 acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg
    39841 ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg
    39901 cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg
    39961 acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga
    40021 ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc
    40081 tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc
    40141 taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca
    40201 agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa
    40261 gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt
    40321 gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac
    40381 ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac
    40441 ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt
    40501 cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt
    40561 aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc
    40621 ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta
    40681 gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta
    40741 ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt
    40801 atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt
    40861 gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg
    40921 ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat
    40981 acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct
    41041 ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa
    41101 gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac
    41161 tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc
    41221 tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt
    41281 tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg
    41341 cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct
    41401 caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt
    41461 atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat
    41521 gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca
    41581 tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg
    41641 aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat
    41701 gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga
    41761 taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga
    41821 accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa
    41881 agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca
    41941 tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg
    42001 agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg
    42061 tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc
    42121 tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa
    42181 cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga
    42241 aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat
    42301 aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga
    42361 gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt
    42421 acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg
    42481 aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta
    42541 ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg
    42601 ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt
    42661 actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg
    42721 cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc
    42781 aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga
    42841 gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt
    42901 aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt
    42961 gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa
    43021 ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac
    43081 cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca
    43141 cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa
    43201 ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat
    43261 atagaagcag gtattgcaca tatccattct acattcaata acactatcat catgatcact
    43321 gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc
    43381 aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg
    43441 gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca
    43501 gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca
    43561 gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt
    43621 ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat
    43681 aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg
    43741 caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg
    43801 tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt
    43861 actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa
    43921 cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat
    43981 taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct
    44041 taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac
    44101 agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat
    44161 tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga
    44221 gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga
    44281 tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt
    44341 ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga
    44401 agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg
    44461 ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc
    44521 tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc
    44581 aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag
    44641 ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt
    44701 gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc
    44761 gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca
    44821 tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa
    44881 tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta
    44941 cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg
    45001 tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat
    45061 gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct
    45121 cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc
    45181 aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa
    45241 ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa
    45301 tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc
    45361 gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg
    45421 gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag
    45481 aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc
    45541 gacagatggt tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag
    45601 aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc
    45661 gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc
    45721 gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg
    45781 atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga
    45841 tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg
    45901 atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc
    45961 gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag
    46021 atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca
    46081 gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt
    46141 agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat
    46201 gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac
    46261 cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga
    46321 acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg
    46381 catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc
    46441 tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac
    46501 attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg
    46561 agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt
    46621 attggatgaa ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt
    46681 ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga
    46741 tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg
    46801 ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc
    46861 tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc
    46921 tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga
    46981 tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc
    47041 cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt
    47101 gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag
    47161 atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag
    47221 ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata
    47281 tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt
    47341 ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt
    47401 acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt
    47461 cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc
    47521 agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca
    47581 ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg
    47641 ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt
    47701 ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct
    47761 ttttgagagg ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc
    47821 aagaaaggat gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca
    47881 caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat
    47941 tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt
    48001 gcatacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa
    48061 gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt
    48121 tcgggaagga cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca
    48181 gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt
    48241 gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa
    48301 aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat
    48361 gcaagttatt ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta
    48421 ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta
    48481 cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca
    48541 tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa
    48601 attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt
    48661 aatgcagcag gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa
    48721 taagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac
    48781 ttctcaaaat tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg
    48841 aatcagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata
    48901 gctcgtcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    48961 tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa
    49021 aatagttcct catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac
    49081 cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa
    49141 aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa
    49201 tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg
    49261 cccttaatga ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt
    49321 tctaattatt tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact
    49381 acttaagatg aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa
    49441 aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc
    49501 aagtatcgag acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat
    49561 ctgaaaaagt tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa
    49621 aatagcaaaa gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat
    49681 tcttaaagta cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct
    49741 atccctttat taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca
    49801 ctaaaacttt ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca
    49861 gtccccaatg gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt
    49921 tatgcaattc atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat
    49981 tatctccagc atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag
    50041 cttcatacca aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa
    50101 caataccgca aacattacct acataattca agatttctcc tctgccatgt ttgccttttg
    50161 ttttaaccaa ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa
    50221 aaccatatag tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt
    50281 taggtaggac ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa
    50341 cagcatcaat accgttgata atatcttcga ctggcattgg caaatcaact agaggattca
    50401 tttgatcttt tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt
    50461 tttccgctaa cattggatca attaaaaatt ttttacctcc atagtctaag atagctgttg
    50521 cattacgaat gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt
    50581 ttagtataat tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt
    50641 tacttaattt atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag
    50701 gaactatcaa aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact
    50761 ggacccgctg tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat
    50821 acagtaaaat taaatatgga aaagctaggg ttttcgattc acgctttttt aattataatt
    50881 acg
//
LOCUS       DACCWV010000005        49966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCWV010000005 DACCWV010000000
VERSION     DACCWV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.005"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..161)
                     /locus_tag="IVW84_000292"
     CDS             complement(<1..161)
                     /locus_tag="IVW84_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7916521.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINR"
     gene            complement(163..360)
                     /locus_tag="IVW84_000293"
     CDS             complement(163..360)
                     /locus_tag="IVW84_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7916522.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(754..2481)
                     /locus_tag="IVW84_000294"
     CDS             complement(754..2481)
                     /locus_tag="IVW84_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7916523.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2474..3667)
                     /locus_tag="IVW84_000295"
     CDS             complement(2474..3667)
                     /locus_tag="IVW84_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7916524.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3854..4498)
                     /locus_tag="IVW84_000296"
     CDS             complement(3854..4498)
                     /locus_tag="IVW84_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7916525.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4592..4726)
                     /locus_tag="IVW84_000297"
     CDS             complement(4592..4726)
                     /locus_tag="IVW84_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7916526.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="IVW84_000298"
     CDS             complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="IVW84_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7916527.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6857..7330)
                     /locus_tag="IVW84_000299"
     CDS             complement(6857..7330)
                     /locus_tag="IVW84_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7916528.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7731..7955
                     /gene="nrdH"
                     /locus_tag="IVW84_000300"
     CDS             7731..7955
                     /gene="nrdH"
                     /locus_tag="IVW84_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7916529.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8114..10273
                     /gene="nrdE"
                     /locus_tag="IVW84_000301"
     CDS             8114..10273
                     /gene="nrdE"
                     /locus_tag="IVW84_000301"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7916530.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10323..11288
                     /gene="nrdF"
                     /locus_tag="IVW84_000302"
     CDS             10323..11288
                     /gene="nrdF"
                     /locus_tag="IVW84_000302"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7916531.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11377..12516)
                     /locus_tag="IVW84_000303"
     CDS             complement(11377..12516)
                     /locus_tag="IVW84_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7916532.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12825..13538)
                     /locus_tag="IVW84_000304"
     CDS             complement(12825..13538)
                     /locus_tag="IVW84_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7916533.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="IVW84_000305"
     CDS             complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="IVW84_000305"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7916534.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14727..16259
                     /locus_tag="IVW84_000306"
     CDS             14727..16259
                     /locus_tag="IVW84_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7916535.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16450..17652)
                     /locus_tag="IVW84_000307"
     CDS             complement(16450..17652)
                     /locus_tag="IVW84_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7916536.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17734..18423)
                     /locus_tag="IVW84_000308"
     CDS             complement(17734..18423)
                     /locus_tag="IVW84_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7916537.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18529..18897)
                     /gene="rplL"
                     /locus_tag="IVW84_000309"
     CDS             complement(18529..18897)
                     /gene="rplL"
                     /locus_tag="IVW84_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7916538.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18950..19453)
                     /locus_tag="IVW84_000310"
     CDS             complement(18950..19453)
                     /locus_tag="IVW84_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7916539.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19863..20552)
                     /gene="rplA"
                     /locus_tag="IVW84_000311"
     CDS             complement(19863..20552)
                     /gene="rplA"
                     /locus_tag="IVW84_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7916540.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20656..21078)
                     /gene="rplK"
                     /locus_tag="IVW84_000312"
     CDS             complement(20656..21078)
                     /gene="rplK"
                     /locus_tag="IVW84_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7916541.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21421..22089
                     /gene="sdaAB"
                     /locus_tag="IVW84_000313"
     CDS             21421..22089
                     /gene="sdaAB"
                     /locus_tag="IVW84_000313"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7916542.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22102..22974
                     /gene="sdaAA"
                     /locus_tag="IVW84_000314"
     CDS             22102..22974
                     /gene="sdaAA"
                     /locus_tag="IVW84_000314"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7916543.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23065..24084)
                     /locus_tag="IVW84_000315"
     CDS             complement(23065..24084)
                     /locus_tag="IVW84_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7916544.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24257..25144)
                     /locus_tag="IVW84_000316"
     CDS             complement(24257..25144)
                     /locus_tag="IVW84_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7916545.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25221..25766)
                     /gene="nusG"
                     /locus_tag="IVW84_000317"
     CDS             complement(25221..25766)
                     /gene="nusG"
                     /locus_tag="IVW84_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7916546.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25867..26037)
                     /gene="secE"
                     /locus_tag="IVW84_000318"
     CDS             complement(25867..26037)
                     /gene="secE"
                     /locus_tag="IVW84_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7916547.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26061..26213)
                     /gene="rpmG"
                     /locus_tag="IVW84_000319"
     CDS             complement(26061..26213)
                     /gene="rpmG"
                     /locus_tag="IVW84_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7916548.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26375..27019
                     /locus_tag="IVW84_000320"
     CDS             26375..27019
                     /locus_tag="IVW84_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7916549.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27075..27578)
                     /locus_tag="IVW84_000321"
     CDS             complement(27075..27578)
                     /locus_tag="IVW84_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7916550.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27591..27637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28175..29140
                     /locus_tag="IVW84_000322"
     CDS             28175..29140
                     /locus_tag="IVW84_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7916551.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29239..30411)
                     /locus_tag="IVW84_000323"
     CDS             complement(29239..30411)
                     /locus_tag="IVW84_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916552.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30398..31543)
                     /locus_tag="IVW84_000324"
     CDS             complement(30398..31543)
                     /locus_tag="IVW84_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916553.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31557..32481)
                     /locus_tag="IVW84_000325"
                     /pseudo
     CDS             complement(31557..32481)
                     /locus_tag="IVW84_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32598..33677
                     /locus_tag="IVW84_000326"
     CDS             32598..33677
                     /locus_tag="IVW84_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7916554.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33709..34302
                     /locus_tag="IVW84_000327"
     CDS             33709..34302
                     /locus_tag="IVW84_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7916555.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34412..34639)
                     /locus_tag="IVW84_000328"
     CDS             complement(34412..34639)
                     /locus_tag="IVW84_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7916556.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34742..37330)
                     /locus_tag="IVW84_000329"
     CDS             complement(34742..37330)
                     /locus_tag="IVW84_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7916557.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37350..38009)
                     /locus_tag="IVW84_000330"
     CDS             complement(37350..38009)
                     /locus_tag="IVW84_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7916558.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38058..38561)
                     /gene="trmL"
                     /locus_tag="IVW84_000331"
     CDS             complement(38058..38561)
                     /gene="trmL"
                     /locus_tag="IVW84_000331"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7916559.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38782..39648)
                     /locus_tag="IVW84_000332"
     CDS             complement(38782..39648)
                     /locus_tag="IVW84_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7916560.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39757..40398
                     /locus_tag="IVW84_000333"
     CDS             39757..40398
                     /locus_tag="IVW84_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7916561.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40460..41821)
                     /gene="mgtE"
                     /locus_tag="IVW84_000334"
     CDS             complement(40460..41821)
                     /gene="mgtE"
                     /locus_tag="IVW84_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7916562.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41839..42738)
                     /locus_tag="IVW84_000335"
     CDS             complement(41839..42738)
                     /locus_tag="IVW84_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7916563.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42740..43537)
                     /locus_tag="IVW84_000336"
     CDS             complement(42740..43537)
                     /locus_tag="IVW84_000336"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7916564.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43515..44219)
                     /locus_tag="IVW84_000337"
     CDS             complement(43515..44219)
                     /locus_tag="IVW84_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7916565.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44350..44925
                     /locus_tag="IVW84_000338"
     CDS             44350..44925
                     /locus_tag="IVW84_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7916566.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            45043..45720
                     /locus_tag="IVW84_000339"
     CDS             45043..45720
                     /locus_tag="IVW84_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7916567.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45799..47607)
                     /gene="pepF"
                     /locus_tag="IVW84_000340"
     CDS             complement(45799..47607)
                     /gene="pepF"
                     /locus_tag="IVW84_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7916568.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47783..48781)
                     /locus_tag="IVW84_000341"
     CDS             complement(47783..48781)
                     /locus_tag="IVW84_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7916569.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49007..49660)
                     /locus_tag="IVW84_000342"
     CDS             complement(49007..49660)
                     /locus_tag="IVW84_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7916570.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14957 a        10482 c         8770 g        15757 t
ORIGIN      
        1 aatctattta ttaaaggacc gggatctaat tcatattttt gtttttctaa ttttaaatat
       61 accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca
      121 ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat
      181 tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca
      241 atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt ttccactatg
      301 agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat
      361 aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca
      421 aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt
      481 taagtatata aactatgtat attctttaat attttttgag aatgcacttc gtacataata
      541 aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa
      601 ttcataatag tttgtcggcg tctttggacg tttcgaagat gagctacgca ttctcttttt
      661 ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat
      721 tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc
      781 ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc
      841 caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc
      901 ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc
      961 cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg
     1021 tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt
     1081 cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat
     1141 cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc
     1201 tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc
     1261 aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata
     1321 ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga
     1381 aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg
     1441 tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga
     1501 taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa
     1561 gatcactact actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt
     1621 tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat
     1681 gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg
     1741 caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac
     1801 cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa
     1861 aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc
     1921 tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag
     1981 agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc
     2041 tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat
     2101 actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga
     2161 gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg
     2221 gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga
     2281 aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag
     2341 taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc
     2401 agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg
     2461 atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca
     2521 cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg
     2581 gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca
     2641 cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat
     2701 ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt
     2761 cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca
     2821 atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa
     2881 attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca
     2941 tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat
     3001 catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt
     3061 gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct
     3121 caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact
     3181 gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt
     3241 tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca
     3301 agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac
     3361 ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac
     3421 tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc
     3481 cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca
     3541 caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca
     3601 tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt
     3661 ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca
     3721 cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac
     3781 tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta
     3841 atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct
     3901 gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg
     3961 ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg
     4021 cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag
     4081 ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct
     4141 gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga
     4201 tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg
     4261 ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat
     4321 ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa
     4381 gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg
     4441 ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt
     4501 tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa
     4561 aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg
     4621 ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg
     4681 cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct
     4741 tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa
     4801 acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc
     4861 aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag
     4921 cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc
     4981 gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta
     5041 tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca
     5101 aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg
     5161 actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg
     5221 aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag
     5281 ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac
     5341 ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc
     5401 aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt
     5461 ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga
     5521 tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg
     5581 aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc
     5641 atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa
     5701 agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag
     5761 tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact
     5821 gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc
     5881 cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg
     5941 atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc
     6001 acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca
     6061 ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata
     6121 aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt
     6181 aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt
     6241 accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct
     6301 cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga
     6361 acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt
     6421 ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt
     6481 tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc
     6541 ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta
     6601 gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc
     6661 acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg
     6721 ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct
     6781 acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt
     6841 ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg
     6901 actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg
     6961 ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct
     7021 tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat
     7081 caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta
     7141 tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata
     7201 aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg
     7261 ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta
     7321 aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata
     7381 tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc
     7441 tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata
     7501 gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt
     7561 tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta
     7621 ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt
     7681 ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa
     7741 aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa
     7801 atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga
     7861 aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat
     7921 tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga
     7981 tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt
     8041 tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag
     8101 gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa
     8161 tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt
     8221 tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttctatcta ttatggaaaa
     8281 aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc
     8341 atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt
     8401 tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta
     8461 tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga
     8521 agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc
     8581 ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca
     8641 agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg
     8701 tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa
     8761 aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt
     8821 cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt
     8881 ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg
     8941 cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa
     9001 aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt
     9061 ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa
     9121 acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg
     9181 ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat
     9241 catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg
     9301 caaacaggaa tatgaagtat taggaacgga tatctcatgt aacttaggct cgacaaatat
     9361 cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt
     9421 gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa
     9481 gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca
     9541 aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa
     9601 ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa
     9661 ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt
     9721 ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga
     9781 agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt
     9841 ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc
     9901 tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc
     9961 gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg
    10021 taaagtaatc gatgtttatg caatggcaca acaacacgtc gatcaaggca tgagtttgac
    10081 tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca
    10141 aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat
    10201 ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag
    10261 ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa aggagcattc
    10321 tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat atcattgata
    10381 aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa
    10441 atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat
    10501 ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta
    10561 aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg
    10621 ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag
    10681 aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg
    10741 aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat
    10801 tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta
    10861 acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg
    10921 gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag
    10981 actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat
    11041 tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca
    11101 aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc
    11161 caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg
    11221 gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat
    11281 tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac
    11341 agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac
    11401 ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg
    11461 gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa
    11521 tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt
    11581 cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa
    11641 aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc
    11701 tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac
    11761 cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc
    11821 aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc
    11881 ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc
    11941 aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa
    12001 aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa
    12061 tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt
    12121 gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc
    12181 atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt
    12241 catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac
    12301 tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa
    12361 accagcaaca agcaatggta aaaaaactgc cgaaagcatt ttcaatattg gactaaaaat
    12421 attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca
    12481 gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat
    12541 acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa
    12601 acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg
    12661 agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg
    12721 catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg
    12781 cttattactt gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg
    12841 actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa
    12901 gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg
    12961 aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta
    13021 agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt
    13081 acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc
    13141 gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa
    13201 gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag
    13261 aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca
    13321 gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc
    13381 aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga
    13441 tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt
    13501 aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata
    13561 tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga
    13621 ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc
    13681 ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa
    13741 tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt
    13801 acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc
    13861 tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc
    13921 gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac
    13981 agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga
    14041 ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc
    14101 gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat
    14161 ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt
    14221 ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc
    14281 atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc
    14341 caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc
    14401 taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc
    14461 ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat
    14521 agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt
    14581 tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc
    14641 aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc
    14701 agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc
    14761 aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc
    14821 ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg
    14881 agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg
    14941 atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg
    15001 caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct
    15061 tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt
    15121 tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag
    15181 gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa
    15241 aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg
    15301 atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga
    15361 tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg
    15421 gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc
    15481 gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt
    15541 atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat
    15601 tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc
    15661 cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg
    15721 atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc
    15781 gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa
    15841 aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc
    15901 tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag
    15961 cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa
    16021 gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga
    16081 caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa
    16141 cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc
    16201 atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa
    16261 cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct
    16321 tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata
    16381 tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc
    16441 tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa
    16501 gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg
    16561 ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat
    16621 aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccttgtactc gtgtttgatt
    16681 ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa gcaaaatggc
    16741 caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa caagtcccat
    16801 atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag gtgcagcaac
    16861 tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc aattgtaggc
    16921 gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc caaacatgaa
    16981 aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat gcggcaaact
    17041 tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac gcggcatttt
    17101 ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat agatcatccg
    17161 ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa tccccacgag
    17221 taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg ctagtaccgc
    17281 accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa aagaagcgca
    17341 tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg cagataacgc
    17401 tgccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa ctaaaaacag
    17461 taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg cacttgtgct
    17521 aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca tattggcaac
    17581 aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag gttttttttc
    17641 attactcttc attgatttcc cccatttcct atcttagttt acaacttatg gaaaagattg
    17701 gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt ttccttgttt
    17761 tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa cacttgcaac
    17821 caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac acgcatacaa
    17881 cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta gaggcaattt
    17941 catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca tatagcctaa
    18001 cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat gattcgtttc
    18061 ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga tgttaccaag
    18121 agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca aaccagcaat
    18181 gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg tcaatacgat
    18241 caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct ctgccttact
    18301 cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca accgatagcc
    18361 cactaccagt atcagaatga ttcccaacga gatcaatatc aatcggatta ttttcttttt
    18421 catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat aaaaacaaaa
    18481 aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt atttaacagt
    18541 tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt ctttagatac
    18601 gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt tcaagcctaa
    18661 accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag ctgcagttaa
    18721 ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag cagctacagc
    18781 tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta ggtcgttaag
    18841 ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca atgccattat
    18901 aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat taagctgcat
    18961 ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg actggcgctt
    19021 gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt gccaatgctg
    19081 tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt tctaatgctt
    19141 ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct tcgttactga
    19201 atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca gcttttttag
    19261 ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca tcacgaagtt
    19321 gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg actactactg
    19381 atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt tttgcgataa
    19441 ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt ttttgcactg
    19501 aaaaggcaag tcacttttat agttactttg ctcaaccagt attattcatc acctattcac
    19561 ttcgttcata gtgattctac tcgctttgct cgaaaggaaa ctcctttcgc tttgctcaac
    19621 cagtattgtt catcatctct tcacttcgtt catagtgatt cacaataaaa aactctatgc
    19681 caccgcagac atagagggac tattgaatat attcaataaa cgtcctcggt aggaaattaa
    19741 ggcttgatgc cacctactgt cttcggtaca agttaattat taacctcaac tagcttagca
    19801 tgctaaacta gttgagtcaa gaattatttt tcagttggat caatcaacat tctaatattt
    19861 aattagaaag aagcttggtc aacatggata ccaggtccaa atgttgttgt gatcgttaca
    19921 tttttcatgt attgaccttt agctgctgat ggtttagctt ttagcaatac atcatggatt
    19981 gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa cttttccgat tggtacatgg
    20041 atatttccag ctttgtccac acggtaagtt actttaccag ctttcacttc gtttactgct
    20101 tttgttacgt ccattgtaac tgtaccagtt ttagggtttg gcattaagcc ttttggtcct
    20161 aatactcgtc ctagacgacc tacagtagcc atcatatcag gtgttgcaac aactacatca
    20221 aagtcaaacc atccgttttg gattttttga accatgtcgt cgtcacctac gaaatctgca
    20281 ccagctgctt cagcttcttt tgctttgtct cctttagcaa aaactaaaac agtttgtgtt
    20341 ttaccagttc cgtttggtaa tacaacagca ccacggattt gttggtctgc ttttttaggg
    20401 tctacgttca aacggtatgc aacttcaact gttgcatcaa attttgcaat gtttgtttct
    20461 tttgctaaag ctactgcttc ggcaacagga taaactttac ttgaatcaac tttttttaat
    20521 gcatcttgca tttttttgct ctttttagcc attttggttc ctccttgatt gtggttataa
    20581 cggttgtacc tcccacgtgt tctatatctt tcaatataga gccaactgag aagctgctgt
    20641 cttagggtgt atagattatt ctacagtgat ccccatgctt cgtgcagttc cttcaaccat
    20701 gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc atttttaatt cagcgatttc
    20761 tcttacttga tcgctagaaa ctttcgcaac tttatttttg tttggttcgc ctgaaccttt
    20821 ttcgatttta gctgcttttt tcaatagaac agcagctggc ggtgtttttg taacgaatgt
    20881 gaatgaacgg tcttcgtata cagaaataac aaccggaatg atcaaacctg cttgatcagc
    20941 tgtacgagcg ttgaattctt ttgtgaatcc catgatattg atacccgctt gacctagtgc
    21001 aggacctact ggtggagctg gagttgcttt acctgcagga atttgcaatt taacgatttt
    21061 ttctactttc tttgccacga gacatacctc cttaagtccg tgatgtggtt aattggagat
    21121 gcatatttct cctcccacgt tttatctgat ctgaaaaaat ccagacaaat aatgtgcaaa
    21181 acaatgcaca ctggtacagt atagcaaatt tatttgtttt ttcaagattt ttttctgcta
    21241 tttttacatt ttgcttgaat aaacgctaaa tttattctaa aaaaacaaca atttttcgtt
    21301 ctcatttctt cttttatgtt acacttgcgc tagcatggtt caacagatga aagcaaacaa
    21361 taacacggtt ttttagaccc ttgtacaaag attcctacat aacaaaatga ggtggagaaa
    21421 atgaaagatt taaggtatag aagtgtcttt gatattattg gacctgtgat gattggtcca
    21481 agtagctctc acactgccgg agctgctaga atcgggaaaa tcatacgtag tatatttggc
    21541 gaacagcctg attctgtaga tatttatcta tatgaatcat ttgcaaaaac ctatcggggg
    21601 cacggaacag atattgcatt agttggcggc ctgttaggga tggaacccga tgatgaacgt
    21661 ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg aagtcttgtt tgttcctaag
    21721 agtgaaaaag ccgaacatcc aaactctgta aagatgcttc taaaaaaagg aacacgaaag
    21781 ctttcagtca ctggcatttc gatcggcggt ggcaatatcc agatttctga attgaatgga
    21841 ttcaaaattt ctttaagcat gggtactcct accttgatcg ttgtccacca agatgttcca
    21901 ggtatgattg ctaaagtgac tagtatactt tctgagacca atacgaatat cggcactatg
    21961 acagtgacac gggaatcaaa aggtgaaaat gccattatga taattgaagt agatgatcct
    22021 caagtagaag aaacggtaaa aagactcaaa cagctgccaa atatcgacag cgtaaattat
    22081 ttcgattaag gagctaaaaa aatgttttta tcgattgaag aattagtaga tcaagcaaaa
    22141 gcttatccaa gtgtagcaga attgatgatt gcagttgaaa tggaaatgag tggccgttcc
    22201 cgtagtcaca tcatcgagat catggaaaaa aacctctcag taatggaaca atcaatcaac
    22261 gagggaaaag aaggtgttac ctccgtcaca ggaattaccg gtggtgatgc aaaaaaatta
    22321 gatgattatt taaatgaggc agacttttta agtggagaaa caatcttaaa agctgtacgc
    22381 aatgctgtag ctgttaatga agtcaatgca aaaatggggt tgatttgtgc tacaccgact
    22441 gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg tccgagatcg gcttgatttg
    22501 acacatgaac agcaattgga attcttattt accgctggcg ctttcgggtt agtcatcgca
    22561 aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag cagaagttgg ttctgcaagt
    22621 gcaatggcag cagcagcttt ggtttgtgca aaaaatggga ctcctcatca agcagcacaa
    22681 gcagtagcca ttacattgaa aaatatgatg ggactgattt gcgatcccgt tgcaggctta
    22741 gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt ctcaagcttt tatctctgct
    22801 gatatggcat tagcgggtat cgaaagtgtc attccgccgg atgaagtcgt tgctgcaatg
    22861 taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa cagcagaagg tggcttagct
    22921 gttacgccaa ctgctaaacg acttgcggaa gagattttaa ataagagggc gtgataaagc
    22981 tccttaattt cgagtattaa gagattagtt atcttcataa caaaaaacga agagaggact
    23041 aattgccctt ctcttcgttt tttattaatc catcaatttc gtatttttta cttcatcgat
    23101 cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt gttcaaaaac
    23161 agatgttact ccttctgccc cacctagatt caacccataa atcactggtc ggccaatcgc
    23221 aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac ggacaccaga
    23281 gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca atacttcaaa
    23341 ggacgcagga cctccatcta gttgacgtcc accatgattg gatacccaga taccatctgc
    23401 ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta cgataactgg
    23461 aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt gttttgcagc
    23521 tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt gttcgctata
    23581 cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt cttcttcacg
    23641 gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg cttctactgc
    23701 tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt ggaaaaactg
    23761 cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat aagtgctgat
    23821 ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc ctttttcatg
    23881 tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt caattccgaa
    23941 aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca tgattttctt
    24001 tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac caccgcgaat
    24061 atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt aggcaatgtt
    24121 tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag atccttcttt
    24181 catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa acatttactc
    24241 tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga tctacttctt
    24301 ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt tcctctattt
    24361 catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac ggtactgttc
    24421 catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga gtggttatag
    24481 gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca gcaggcccct
    24541 ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta aaaggcgcgc
    24601 caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac gttcctaaat
    24661 agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta tctatgccgt
    24721 agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc cactcgttat
    24781 ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca gacaaatctc
    24841 ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt actgcttgct
    24901 gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg ctgtagccgt
    24961 ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct gccgtttttt
    25021 ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt aacgcaaaca
    25081 aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg attggttctg
    25141 tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca atcatatgat
    25201 cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc aagttctgta
    25261 ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc tccgtcaact
    25321 tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac gacttcgcca
    25381 acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa gatatgattg
    25441 acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc aacgaatcct
    25501 gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat catttcaaca
    25561 agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt tttgacttca
    25621 gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt catactttgc
    25681 gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt atgcaagaca
    25741 taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa aataaaaaaa
    25801 cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta cgttgtggct
    25861 agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca ttacgtaaaa
    25921 catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac ggagttgttt
    25981 tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa atttcatgag
    26041 acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg attacaatat
    26101 ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac tgattttgtg
    26161 tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc catttacttc
    26221 tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac tttttacttg
    26281 tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga ttaatttgaa
    26341 attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac ttgtatataa
    26401 gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat taaaaatttt
    26461 agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata ttttccgtgg
    26521 aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata tgacgcttcc
    26581 tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt ctttcttcaa
    26641 agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg aaaatcattt
    26701 ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt ggagcgtaga
    26761 acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt tggctgctat
    26821 tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg atatcgaaaa
    26881 agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag cgaaagtatg
    26941 tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa ttgaaaattt
    27001 gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct gctttctttt
    27061 ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa ataatacatc
    27121 aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc ataggttgtc
    27181 caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat gctgatggta
    27241 aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt ttgcaataaa
    27301 gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc catgatacca
    27361 aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag caatttacgg
    27421 ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg ccctgaggat
    27481 acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc atttacaacg
    27541 attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct tttctcctag
    27601 tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa taattataac
    27661 agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa gcttaaacaa
    27721 tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg ggtcataaga
    27781 attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg aaacttaaat
    27841 aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc tagcaagcag
    27901 ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag taatggatca
    27961 tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt ttcctagact
    28021 tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa caagtttctt
    28081 tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca ggagtattaa
    28141 aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc atatattgat
    28201 caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt taccggtatt
    28261 aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac acgatgctga
    28321 gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag aacagaaaac
    28381 aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac atcaggtact
    28441 tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag caggttctgg
    28501 agatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac ttcagctgat
    28561 tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa aaataagtga
    28621 aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta tcgtccatgc
    28681 gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat cattttcgta
    28741 tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc tggtagatgc
    28801 aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga agcatccttt
    28861 ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag tcattgattt
    28921 tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt tacttataca
    28981 gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat tacgtatcgt
    29041 ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac gaccttttga
    29101 tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt tttgtcagaa
    29161 gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt ttgtcacagt
    29221 ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat agtttctttc
    29281 tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta agacacaacc
    29341 attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac tgatcaagtt
    29401 attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa ccatttctgg
    29461 caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga taaaaatatc
    29521 tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag ccgaaaagct
    29581 aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg ttatatccca
    29641 cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca acagcagcga
    29701 gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa tgggcgcact
    29761 ctggtttcgt ttactgatct ctttgcgatt aaatgctaga ttgatgacgc tgaaaccgct
    29821 aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg tcaacagctt
    29881 tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg agagtgccat
    29941 ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca gaatcagtgt
    30001 ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat ctgggcgact
    30061 ctgtatcttg atcgtcggca tctttcctac ttcgacttgt tcggtaaagt ctgcaggcag
    30121 atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt tttgtgaggt
    30181 tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc catcgactac
    30241 aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg tattttcaac
    30301 atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac ctatcagtaa
    30361 attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca taacgcaccc
    30421 ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc aaccatagaa
    30481 gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa ctttcgctga
    30541 tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc caatacttca
    30601 cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact gaaatcaaga
    30661 tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat gtacctaaca
    30721 aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa cgttcaccaa
    30781 agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct gtcaaaacaa
    30841 tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
    30901 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
    30961 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
    31021 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
    31081 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
    31141 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
    31201 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
    31261 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
    31321 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
    31381 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
    31441 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
    31501 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
    31561 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
    31621 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
    31681 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
    31741 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
    31801 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
    31861 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
    31921 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
    31981 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
    32041 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
    32101 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
    32161 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
    32221 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
    32281 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
    32341 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
    32401 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
    32461 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
    32521 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
    32581 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
    32641 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
    32701 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
    32761 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
    32821 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
    32881 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
    32941 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
    33001 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
    33061 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
    33121 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
    33181 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
    33241 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
    33301 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
    33361 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
    33421 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
    33481 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
    33541 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
    33601 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
    33661 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
    33721 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
    33781 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
    33841 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
    33901 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
    33961 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
    34021 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
    34081 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
    34141 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
    34201 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
    34261 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
    34321 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
    34381 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
    34441 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
    34501 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
    34561 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
    34621 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
    34681 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
    34741 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
    34801 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
    34861 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
    34921 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
    34981 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
    35041 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
    35101 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
    35161 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
    35221 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
    35281 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
    35341 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
    35401 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
    35461 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
    35521 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
    35581 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
    35641 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
    35701 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
    35761 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
    35821 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
    35881 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
    35941 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
    36001 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
    36061 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
    36121 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
    36181 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
    36241 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
    36301 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
    36361 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
    36421 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
    36481 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
    36541 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
    36601 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
    36661 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
    36721 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
    36781 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
    36841 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
    36901 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
    36961 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
    37021 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
    37081 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
    37141 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
    37201 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
    37261 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
    37321 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
    37381 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
    37441 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
    37501 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
    37561 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
    37621 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
    37681 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
    37741 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
    37801 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
    37861 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
    37921 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
    37981 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
    38041 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
    38101 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
    38161 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
    38221 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
    38281 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
    38341 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
    38401 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
    38461 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
    38521 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
    38581 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
    38641 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
    38701 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
    38761 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
    38821 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
    38881 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
    38941 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
    39001 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
    39061 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
    39121 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
    39181 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
    39241 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
    39301 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
    39361 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
    39421 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
    39481 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
    39541 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
    39601 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
    39661 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
    39721 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
    39781 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
    39841 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
    39901 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
    39961 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
    40021 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
    40081 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
    40141 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
    40201 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
    40261 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
    40321 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
    40381 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
    40441 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
    40501 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
    40561 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
    40621 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
    40681 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
    40741 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
    40801 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    40861 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    40921 cggtcgacat acctaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    40981 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    41041 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    41101 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    41161 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    41221 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    41281 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    41341 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    41401 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    41461 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    41521 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    41581 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    41641 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    41701 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    41761 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    41821 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    41881 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    41941 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    42001 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    42061 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    42121 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    42181 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    42241 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    42301 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    42361 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    42421 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    42481 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    42541 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    42601 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    42661 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    42721 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    42781 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    42841 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    42901 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    42961 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    43021 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    43081 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    43141 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    43201 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    43261 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    43321 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    43381 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    43441 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    43501 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    43561 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    43621 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    43681 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    43741 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    43801 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    43861 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    43921 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    43981 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    44041 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    44101 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    44161 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    44221 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    44281 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    44341 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    44401 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    44461 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    44521 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    44581 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    44641 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    44701 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    44761 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    44821 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    44881 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    44941 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    45001 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    45061 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    45121 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    45181 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    45241 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    45301 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    45361 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    45421 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    45481 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    45541 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    45601 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    45661 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    45721 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    45781 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    45841 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    45901 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    45961 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    46021 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    46081 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    46141 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    46201 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    46261 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    46321 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    46381 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    46441 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    46501 atagcttccc gaagaataag catcgcttcg tttcccttta ttttcgacaa catcgatcca
    46561 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    46621 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    46681 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    46741 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    46801 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    46861 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    46921 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    46981 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    47041 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    47101 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    47161 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    47221 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    47281 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    47341 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    47401 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    47461 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    47521 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    47581 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    47641 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    47701 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    47761 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    47821 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    47881 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    47941 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    48001 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    48061 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    48121 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    48181 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    48241 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    48301 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    48361 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    48421 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    48481 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    48541 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    48601 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    48661 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    48721 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    48781 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    48841 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    48901 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    48961 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    49021 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    49081 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    49141 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    49201 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    49261 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    49321 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    49381 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    49441 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    49501 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    49561 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    49621 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    49681 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    49741 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    49801 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    49861 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    49921 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DACCWV010000006        43644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCWV010000006 DACCWV010000000
VERSION     DACCWV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.006"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(4..348)
                     /locus_tag="IVW84_000343"
     CDS             complement(4..348)
                     /locus_tag="IVW84_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7916571.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(341..775)
                     /locus_tag="IVW84_000344"
     CDS             complement(341..775)
                     /locus_tag="IVW84_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7916572.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            913..1650
                     /locus_tag="IVW84_000345"
     CDS             913..1650
                     /locus_tag="IVW84_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916573.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1643..2854
                     /locus_tag="IVW84_000346"
     CDS             1643..2854
                     /locus_tag="IVW84_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7916574.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3061..3840
                     /locus_tag="IVW84_000347"
     CDS             3061..3840
                     /locus_tag="IVW84_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7916575.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            3910..4572
                     /gene="trmB"
                     /locus_tag="IVW84_000348"
     CDS             3910..4572
                     /gene="trmB"
                     /locus_tag="IVW84_000348"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7916576.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4670..6424)
                     /gene="ade"
                     /locus_tag="IVW84_000349"
     CDS             complement(4670..6424)
                     /gene="ade"
                     /locus_tag="IVW84_000349"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7916577.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6556..7485
                     /gene="cpaF"
                     /locus_tag="IVW84_000350"
     CDS             6556..7485
                     /gene="cpaF"
                     /locus_tag="IVW84_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7916578.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7442..8491
                     /locus_tag="IVW84_000351"
     CDS             7442..8491
                     /locus_tag="IVW84_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7916579.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8488..8766
                     /locus_tag="IVW84_000352"
     CDS             8488..8766
                     /locus_tag="IVW84_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7916580.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8741..9211
                     /locus_tag="IVW84_000353"
     CDS             8741..9211
                     /locus_tag="IVW84_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7916581.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9228..9530
                     /locus_tag="IVW84_000354"
     CDS             9228..9530
                     /locus_tag="IVW84_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916582.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9547..9918
                     /locus_tag="IVW84_000355"
     CDS             9547..9918
                     /locus_tag="IVW84_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7916583.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9890..10249
                     /locus_tag="IVW84_000356"
     CDS             9890..10249
                     /locus_tag="IVW84_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916584.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10421..11428
                     /locus_tag="IVW84_000357"
     CDS             10421..11428
                     /locus_tag="IVW84_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7916585.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11453..12637
                     /locus_tag="IVW84_000358"
     CDS             11453..12637
                     /locus_tag="IVW84_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7916586.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13074..14657)
                     /locus_tag="IVW84_000359"
     CDS             complement(13074..14657)
                     /locus_tag="IVW84_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7916587.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15235..15378)
                     /locus_tag="IVW84_000360"
     CDS             complement(15235..15378)
                     /locus_tag="IVW84_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7916588.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15601..16080)
                     /locus_tag="IVW84_000361"
     CDS             complement(15601..16080)
                     /locus_tag="IVW84_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7916589.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16153..16437)
                     /locus_tag="IVW84_000362"
     CDS             complement(16153..16437)
                     /locus_tag="IVW84_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916590.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16515..16709)
                     /gene="ssrS"
                     /locus_tag="IVW84_000363"
     ncRNA           complement(16515..16709)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVW84_000363"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16781..18067)
                     /locus_tag="IVW84_000364"
     CDS             complement(16781..18067)
                     /locus_tag="IVW84_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7916591.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18205..18876
                     /locus_tag="IVW84_000365"
     CDS             18205..18876
                     /locus_tag="IVW84_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7916592.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18842..19330
                     /locus_tag="IVW84_000366"
     CDS             18842..19330
                     /locus_tag="IVW84_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7916593.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19343..20290
                     /gene="prmA"
                     /locus_tag="IVW84_000367"
     CDS             19343..20290
                     /gene="prmA"
                     /locus_tag="IVW84_000367"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7916594.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20291..21055
                     /locus_tag="IVW84_000368"
     CDS             20291..21055
                     /locus_tag="IVW84_000368"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7916595.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21288..23501
                     /locus_tag="IVW84_000369"
     CDS             21288..23501
                     /locus_tag="IVW84_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7916596.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23512..23958
                     /locus_tag="IVW84_000370"
     CDS             23512..23958
                     /locus_tag="IVW84_000370"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7916597.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24129..25388
                     /locus_tag="IVW84_000371"
     CDS             24129..25388
                     /locus_tag="IVW84_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7916598.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25477..26424)
                     /locus_tag="IVW84_000372"
     CDS             complement(25477..26424)
                     /locus_tag="IVW84_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7916599.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26818..28110
                     /locus_tag="IVW84_000373"
     CDS             26818..28110
                     /locus_tag="IVW84_000373"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7916600.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28125..29894
                     /gene="aspS"
                     /locus_tag="IVW84_000374"
     CDS             28125..29894
                     /gene="aspS"
                     /locus_tag="IVW84_000374"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7916601.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30003..30878
                     /locus_tag="IVW84_000375"
     CDS             30003..30878
                     /locus_tag="IVW84_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7916602.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31144..33027
                     /locus_tag="IVW84_000376"
     CDS             31144..33027
                     /locus_tag="IVW84_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7916603.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33102..33386)
                     /locus_tag="IVW84_000377"
     CDS             complement(33102..33386)
                     /locus_tag="IVW84_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7916604.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33376..34110)
                     /locus_tag="IVW84_000378"
     CDS             complement(33376..34110)
                     /locus_tag="IVW84_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7916605.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34239..34862
                     /locus_tag="IVW84_000379"
     CDS             34239..34862
                     /locus_tag="IVW84_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7916606.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35054..36175
                     /locus_tag="IVW84_000380"
     CDS             35054..36175
                     /locus_tag="IVW84_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7916607.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36182..39310
                     /locus_tag="IVW84_000381"
     CDS             36182..39310
                     /locus_tag="IVW84_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7916608.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39329..42523
                     /locus_tag="IVW84_000382"
     CDS             39329..42523
                     /locus_tag="IVW84_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7916609.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42669..43376)
                     /locus_tag="IVW84_000383"
     CDS             complement(42669..43376)
                     /locus_tag="IVW84_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7916610.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14071 a         7750 c         9225 g        12598 t
ORIGIN      
        1 ttattatatt tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt
       61 gatttgtgtg atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc
      121 ttcttggaaa gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt
      181 aaaatgattc aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc
      241 gatcagtttc tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt
      301 gccagcagca gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct
      361 gctttacgat tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat
      421 tgacaaagtg aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg
      481 aatgaaaaac agattgatag gctagttcac ggttgttatt caagatatta agacctttca
      541 tttctggaaa agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc
      601 ctgcaagttc gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg
      661 tgtgtccttt cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata
      721 ttttatagct tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa
      781 acatcctttc tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg
      841 taaggaaaaa aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc
      901 ggaggaaaaa gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct
      961 gtgttaaaag atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg
     1021 aacggagcag ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa
     1081 ggaacgatag aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa
     1141 attgggtata ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc
     1201 gaagtcactg cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag
     1261 ttgttggaga cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag
     1321 ggaatgaaac agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc
     1381 atcgacgaac catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg
     1441 gtagaaatga aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca
     1501 gaaaaatatt gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct
     1561 cttgagatgt tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt
     1621 gctttaacaa aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca
     1681 aaagaaaatg atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct
     1741 tttcttgatt ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc
     1801 gtttgcaatt ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa
     1861 gatggctact tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat
     1921 gcaaacctat ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat
     1981 attggcatgc ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat
     2041 gtttttcgtc tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag
     2101 agcagaattg tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt
     2161 ttcttctctg atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg
     2221 tgcattagta caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt
     2281 ggattgggag aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat
     2341 caatttattc acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga
     2401 tcctatattg actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag
     2461 aagattggca agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg
     2521 gttattgatt gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt
     2581 atatttgatc ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt
     2641 tcagctttat ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg
     2701 ggtattaatt atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca
     2761 gcagcgatgg gttccgctag cggcttattt gattgttact atgctctttg ttaaactata
     2821 tgtaccaact cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg
     2881 agaatgaaaa atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa
     2941 gctggttttt tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt
     3001 tcaggcgact agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg
     3061 atggagtttc agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa
     3121 acatacatag gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg
     3181 ttggctgctc tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg
     3241 aatggcgata ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag
     3301 attggtcaaa gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt
     3361 aaaaccatgc tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa
     3421 tatgaaaaag aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt
     3481 tttcttcgtg agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat
     3541 gtaaaccatc gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca
     3601 atcatgtttg cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg
     3661 tctagctgga atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa
     3721 aaatttcatt ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt
     3781 gaagatcatc gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga
     3841 ggtttggccc aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg
     3901 agataaataa tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg
     3961 aattttgtaa ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac
     4021 gatcatccaa tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca
     4081 aaagcccatc ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt
     4141 gcactagaca agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga
     4201 tcagcgttga cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc
     4261 gatccatggc caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct
     4321 gtagatgaac agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga
     4381 ttgtttgagt attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg
     4441 ttagatttac atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt
     4501 tcttcaaaag gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag
     4561 aaaagtctgt gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatcc
     4621 cagaaaatga acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat
     4681 ttatttcatc ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc
     4741 ctttgtcggt gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa
     4801 aaggatcaaa ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact
     4861 gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac
     4921 aagctatttc ctgctgattg ttgacaacgg tcatcccgcc acctttttga atcaaaagat
     4981 tagcagcata cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat
     5041 gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc
     5101 caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga
     5161 ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta
     5221 gactattcgg gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg
     5281 aatgtggatt taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat
     5341 gatcagcaaa taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga
     5401 aaacttcatg aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc
     5461 caggggcaat cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca
     5521 tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga
     5581 ttgatccttc ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc
     5641 ttgaattttc aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt
     5701 ctcgaaccat taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag
     5761 cttcatgatc cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac
     5821 cagctgcatg cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga
     5881 tgtctgtttc attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt
     5941 cgggtttttg taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa
     6001 aaggggttac aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg
     6061 catcttctat cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat
     6121 ctgtcactac agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata
     6181 aaagtgagct ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat
     6241 tagcattatg tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga
     6301 ttgctacatc tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga
     6361 tcactaggtc agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt
     6421 ccaatggagt gcctcccaag ataaattttt ataagtataa cataaattcg gtttttgcca
     6481 atattttttt gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt
     6541 gtggagggga gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt
     6601 gccaaacatg cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt
     6661 cgctatggaa aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg
     6721 atctcaagat ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta
     6781 ggtgcattta cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga
     6841 gattaccttc aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc
     6901 catcgttgct ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga
     6961 ttataccttt tcagcggacc agtcggatca ggcaagactt ctctaatgta tcggttggct
     7021 ttggaagaaa aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt
     7081 tttctgcagc tacagatcaa tccgaaaata gcacagactt atgatacact tttgagactc
     7141 gcattacggc atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca
     7201 catgctgcgg taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc
     7261 aatcttgaag gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct
     7321 gaatgtttag ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct
     7381 gttttatgga gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt
     7441 tatgaagaag ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag
     7501 ccagcaaaac cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca
     7561 agaatccctt tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat
     7621 ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg
     7681 agcagagcaa ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt
     7741 gttggaaatt gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct
     7801 gcttgcatat ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt
     7861 gttcgttttg cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca
     7921 gctgatacaa gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc
     7981 tttactagta aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag
     8041 cagacaatta ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga
     8101 gattggaaag ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga
     8161 aacgaaacaa gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc
     8221 aggtatgtca ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa
     8281 aattgtttta cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga
     8341 atggacaaga aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat
     8401 catttttttg ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt
     8461 ttacggaagc atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt
     8521 tttacattga tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc
     8581 gtccctaatt tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc
     8641 cagttggtag acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact
     8701 gtggaggaat tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg
     8761 ccttgaaggt aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt
     8821 attacttaca acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt
     8881 ggcagtctat caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc
     8941 tattgtgaat cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt
     9001 tcatgttccg aatccagata aaataaattg ggaagtatta gatattcctg atgagattac
     9061 cttgaaacgc catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa
     9121 agcttatcaa ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg
     9181 gagtggtcgc tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta
     9241 cagcgcttat gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc
     9301 ttcaggcaga aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata
     9361 tcaagacgca gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg
     9421 atatccaaat tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag
     9481 aagcgatcag tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt
     9541 ggactgatga tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc
     9601 caagtaagtg aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa
     9661 ttagaagaag aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac
     9721 acgatcgagg ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga
     9781 acttccggcg gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa
     9841 gaacacacaa tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg
     9901 acaaaaaacg atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt
     9961 atttctttta tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga
    10021 tcttaatata cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc
    10081 ggaacagtct acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt
    10141 aacatatcag tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt
    10201 tcggttcaca gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg
    10261 tgaaaacgcc ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca
    10321 caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg
    10381 tgaaaaaatt gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga
    10441 acaagggttt aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc
    10501 ctttttagaa gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt
    10561 ggtggatgga gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt
    10621 gaagaaactt tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa
    10681 gggcagccag acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt
    10741 tttatttgtt tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct
    10801 ggatattgcg gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc
    10861 ggggtaccaa acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc
    10921 ttctacaagt gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga
    10981 attattaata gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa
    11041 tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca
    11101 tttattgcta gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat
    11161 gccgagtgga tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga
    11221 aggttatctt caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca
    11281 aaaaagtatt ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt
    11341 gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt
    11401 taaaaactgg aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa
    11461 aacaattgca atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga
    11521 agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac
    11581 catcaagtac ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt
    11641 agcagtaaaa atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga
    11701 aatcaccgga gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt
    11761 gatcgatgag aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa
    11821 tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt
    11881 ggctgtattc gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt
    11941 accggtgtct tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca
    12001 tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat
    12061 catcacttgc cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat
    12121 ggatacttct atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga
    12181 tatcgatcca gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat
    12241 gatcaatgtt ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat
    12301 gcgtgatctt gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt
    12361 ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat
    12421 tgtctttact gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg
    12481 tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt
    12541 agatatctct acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact
    12601 aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa
    12661 agatgaaacg catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa
    12721 aatggtaatt taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct
    12781 tggaaaagtg gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc
    12841 caatatttta ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat
    12901 ttttagatca gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac
    12961 cgatccgaaa cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct
    13021 gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca
    13081 atcagttggc cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg
    13141 aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct
    13201 tgcagcattt cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc
    13261 aaaatcagga cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca
    13321 ccaccggata aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct
    13381 aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg
    13441 ctatcgggat cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct
    13501 aatttgatgg tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat
    13561 tttcctgcac cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg
    13621 ctgtctttga atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact
    13681 tctttgccag attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc
    13741 ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag
    13801 gactcattgt ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc
    13861 tggacttctt tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc
    13921 aagactcgaa aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc
    13981 catgtttttg tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat
    14041 ttttttgtat tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg
    14101 gtcggttcat cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact
    14161 ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt cctgacggtg tggaatccct
    14221 aagccttgca agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag
    14281 cgatgctgca gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc
    14341 atcaatgctt cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg
    14401 agcaagtagt ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc
    14461 tttaagcctt ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt
    14521 tgtaaaaagg ttgatttgcc agttccgttg ataccgacta aggcaatttt ttcaccagct
    14581 tcaatttgac aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa
    14641 attgcttgta tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatcca
    14701 tggcctgtgt tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat
    14761 tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa
    14821 ttgcatggct aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca
    14881 tgcgcatcat agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg
    14941 tcagaaagtt caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag
    15001 agagcttttt ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc
    15061 taaaccagcg ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt
    15121 ccttttgcca tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc
    15181 aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt
    15241 cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa
    15301 ttttagtttg gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga
    15361 ttcatcggaa acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa
    15421 accacaccag ggcgactaat cgccctggtg ttcacttttt tttattttac ttgaactcat
    15481 tgactgtttt ccctttattt taaacattat aaagaccgat gagtgcttgc cgcgtctttt
    15541 gtttttatca cggttttttg tgcaaaaaaa cctgccgata gttggcaggt cgtctacgtg
    15601 ttaagctttg gtgtggtctt tagctggcaa ttggttttct aaatcagttc ggacaacaag
    15661 cacatcgcaa gctgcgtttc taataacata ttcagaaaca gaaccaataa ataaacgttc
    15721 tacagcattc aatcctgtgg ctcctaacat aatcaaatct acttgattat cttctgggat
    15781 ttctctggca ataatttgtt ttggtgagcc atattctacg acagaggtta cattattcaa
    15841 gccagctttc ttcgcattgc tttcataatc tgccaatgtt tgctttgcca tttcagtagc
    15901 ttgttctgca agcattccat caaaggatga aacggtttgg aaagaacgtg tgtcaatgac
    15961 atgtgctaat agaagttctc cgttatttcg gatagctaca ttgaccgcct ttttaaaagc
    16021 taattcagct tcatcagaac catctacggc aaccatgatt tttttgtatt gttgtaacat
    16081 aagcattcac tcctcaagtc aatcgtattg tactctttta ttgtagaaca ctttagggaa
    16141 aaagagaatt atttactgct ttggttcagc aatttttttt gcaaatcgga tactgaaggt
    16201 ggcagaaaca ataattacga tcagtaaaaa gaccaaggca ataaagcgtt gattgaaaaa
    16261 agctgttaaa atacccatta cgcctaaaac agcatatgca gctccgtaaa attgtaggaa
    16321 tccttgattt ttatcatttt tgttgatcaa tacaaaaaag atatcctgtt tattaaataa
    16381 ataatagcta acgaataata atactaaagt ggcgataatc attaaaatcc taatcatgaa
    16441 caaatcctcc aaaataagca acaaatagaa aaagcaattt gccaagagca aattgcttta
    16501 ttctttaaaa gatggtaatc tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc
    16561 gaattcaagc aggtgggtcc catggtcgta gcttcgaact accaaatgat aaggcatgtt
    16621 accagcagtg acacaagacg ggtcccaaga tatcaataaa aatgttcggt caacacacaa
    16681 agaggacaat tcgaacatca cagatttccc acctctatag tagcatatga atttaaaaag
    16741 agcaatgaga gagctcgcag aaacagaacc gttttcttgt ctagtttttt ggaggatgct
    16801 ttttccactc ttttatacgg taatactgtt gacccagagc ctgttcatat ttccctgtat
    16861 cttttggctg ataatactga gcattcttca gtttatctgg taaatattgc tgattgaccc
    16921 aagcctgatc aaaatgatga ggatactgat aacccacgcc acggttgagt gatttcgcac
    16981 ctttgtaatg gctatctctc aaatgatcag gaacatctcc agctttgccc tctcgaatat
    17041 cagcaagtgc tgcatctaat gccatgtagg cagaattgga ttttggcgac aagcaaagat
    17101 cgacgacgac gtctgctagt gggatacgtg cctcaggcaa tcccaatttt tcagcagcta
    17161 atacagcgtt cactgtacga gccgcagcag caggattacc taaacctata tcttcataac
    17221 cgataaccat cagacgccgg caaatacttg ccagatcacc tgcctcaact aaacgggcga
    17281 gataatgaag tgcagcatcc acatcacttc cacgaatcga tttttgaaaa gccgaaataa
    17341 cgtcataatg agcatctcca tttttgtcat gggtaagagc ttttctttgg acacattctt
    17401 caatgatcga cagtgtaaga tgaatccttc cttctttatc tgaaggagtc gatagtgtag
    17461 ccagttccag cccattcaat gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa
    17521 gaagagcatc ttcgtctaac tggattgctt gttgtccaag gccgcgttct ttatccctta
    17581 aagcatgttc gactgccaac tggatgtctt gttctgttaa tggtttgact tcaaagatct
    17641 gtgtacggct gcggatcgct ggattgatgg taatataagg attctcagta gtcgctccga
    17701 tcaagatgat tcggccactt tctaaatgag gaagcagaaa atcctgtttt gttttatcta
    17761 atcggtgtac ttcatccaat aagaggatca ctgtaccact catttttgct tcttccgcta
    17821 caacttgtag atcctttttt gaatctgtag cggcgtttag catgcgaaat gcataattag
    17881 tagagccggc gatcgcactt gcgatactcg tttttccagt accaggaggt ccatatagaa
    17941 tcatggaaga gagcatgcga gcatcgacca tgcggcgtat gatttttcct tccccgacta
    18001 gatgctgctg accaacgact tcatcgatcg ttcgaggtct catgcgataa gccaatggct
    18061 gttgcatagg attttcctcc ttcttgtttt tacgtccctt aattatatca tatttagcaa
    18121 gatgcctaga aagaacagcc ttctgttgtt ttgttttcat agcttatcca actaatattg
    18181 tgtatgataa agtaggtgag aaaaatgacg gataaacaaa agatagatga atttttttgg
    18241 aataaaacga cgccagaagt ggcacgggat ttattgggga tgtatttgga gcatgacaca
    18301 ccagaaggaa gattagctgg atatattgta gacgcggaag catacctagg tccagaagac
    18361 gaggccgccc atagctatgg tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa
    18421 ccaggtacga tctaccttta cacgatgcat actcatcgga ttttgaacat cattactcag
    18481 ccagaaggga ttcctcaagg agtaatgata cgtgcaatcg aacccgccgc aatgattgat
    18541 caaatgagta aaaatcgtgg cggaaaaact ggtccagata tcagcaacgg tccgggaaaa
    18601 ttagtggaag cattagcaat tcctcaagaa ctttatggtc agtctatagc agatagttcg
    18661 ttacgcttag tttttgagaa aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc
    18721 ggtatcccaa acaaaggtgt atggacagaa aagccgttgc gttttgttgt gtcgggaaat
    18781 ccttatatct ctttacaaag aaaaaaccaa atcgagaaag attggggttg gaggaaagaa
    18841 aatgaaaaag aaggatctat taacatattt agatgaaata attgaaaaac aagtgacaga
    18901 ctatgatgta gctttagatt gggatacaaa aaatcatacg atcgagatcg ttgttcgctt
    18961 gtttgcagaa aataaagcac aatttgagat tgatgatgca gaagggatcg tttcgcaaga
    19021 accaattatc gaatttgaag acggcgtact tctttttaat cctcagaaat ctgtttttga
    19081 tgaacgcaat tatctagcag taatccctta tgaaggaaaa aaagggttgg caaaatcagt
    19141 agctgattct ttagtggaat atctaaatga agtattagct caagggcaaa gcgatttgct
    19201 tgattttttg gatgaagacg atgacgaagc tgtattcgag cttcactggg ataatcagca
    19261 actagcagaa ttagtagaag taaaacagca aaatgaagca gatacttatt taccgtaccc
    19321 aagttattaa ggagtgacag atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga
    19381 agcagtcgaa gcagttgcta atattttgat ggaggcaggg gcgagtggtg tcgcgattga
    19441 agattcttta gatgttgaac attttcagtc ggatcaatat ggtgagattt tagataaaaa
    19501 tgattttaca tcgctgaaag aaggcgcact agtcatggct tattttccgg aaacgacttt
    19561 cttaccggaa atcttgcctt ttatcaaaga tcgtatcaca caattacctg agtttggtct
    19621 agctattgga aaaaataaag taacagttag tgaagtagag gaaagcaact gggcaacagc
    19681 ctggaagaaa tactatcatc cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg
    19741 ggaaaactac cagacagaag atcctctaga aaaaattatc acactagatc ctggtatggc
    19801 atttggcaca ggcactcatc ccacgacttc cttgacattg acagcactag aaacagtctt
    19861 gcgtggtggt gaaacattgc tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc
    19921 aaaatatctt ggtgcaaaag atgtctatgc ctttgattta gacgaagtag ccgttcgttc
    19981 ggcgaaagaa aatatggata tgaatgaaat agccaaagat gtccatgtat cagctaacga
    20041 tcttttgaca ggaatcgaga ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat
    20101 catcttattg atgatcccag atgcttggcg tttactgaag caaacaggca cgctgatcgt
    20161 ttcaggaatc attgaagaga agaagcaaat ggttctggat gccatgttag aacaagggtt
    20221 tatcgtggat caaattttcc agcaaaaaga ctggtatgca attattttga aaaaaccaga
    20281 ggtggaatag atgcagcggt attttttaaa agaatcatat gaatcaaaag actattttgt
    20341 tttagaaggg gaaaactatc accacattgt acgggttatg aggatggaac ccgaggatga
    20401 agtttttttg gcattcaatg atcaaatagc cattgttgca gaaatcacgg agattactga
    20461 agaggctgtt tatctgaaag aaattaaaaa agaagaaaat caaaaggaac tgccaagttc
    20521 gattaccata gcgagtggct atcctaaagg ggaaaagctg gattggatcg ttcagaaatc
    20581 cactgaatta ggagcgcaag cttttcttgg tttcccggca tcagcctctg tcgttaaatg
    20641 ggatgcaaaa aaacgtatga aacgacaaca acggcttgaa aagattgctc aagaggcagc
    20701 ggaacaatcc catcggcaga tcgtgccatc agtagagtta ttggaaacga aacaagattt
    20761 atttgagcgg ctagatgatt atgacgtggt cttggttgct tatgaggaat cagcaaagca
    20821 aggcgaaaaa tcaaatgcag ccaaaattct tagtgaaaca aaaccagata gtcgaatctt
    20881 agctgtcttt ggaccagaag gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca
    20941 caaagggata ctttgtggat taggaccaag aatccttcgt acagaaacag cacctcttta
    21001 tttattaagt gctgctagct atcaatggga attaatgaat ccttctttca gctaacgttc
    21061 ttcattaata aaagagtatc gacagaaaga attgaaaaaa tagtcaacaa tttgtataat
    21121 agaaacaatt atttatacaa tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga
    21181 acatttccgt aaaaaaacgt ataactgcac ggattttggg acaattatca aacagatatt
    21241 tgtcccgatt cccgttttac cgaaaaaaga aagggggatc gattcctgtg acaaaagaag
    21301 aaattttaac gggacccggt gtcataaagc tcgttagtca atacatgggt cccgaacatg
    21361 tagcatttgt tgagaaagca tgcgaatacg cgactgctgc acatgacgga cagtttagaa
    21421 aatcaggcga accctacatt attcatccta tccaagtcgc aggtatatta gcagatttaa
    21481 aaatggatcc ccatacagtg gctacaggct tcttacatga tgttgttgaa gatacagaaa
    21541 tcactttaga agatctgaga gaagaatttg gcgatgacgt tgctatgtta gtggacggcg
    21601 taaccaaatt agggaaaata aaatataaat cccacgaaga gcagctagca gaaaaccacc
    21661 gaaagatgct gctagcaatg gctcaagatt tacgagttat catggtcaaa ttagctgata
    21721 gactacataa catgcgtacg ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc
    21781 aggaaacttt agaaatctat gcgcctcttg cacatcgtct agggatcagc cggatcaaat
    21841 gggaattaga agatacagca cttcgttatc taaatccaaa acagtattac cgtatcgtcc
    21901 acttgatgca gacgaagaga gaagaacgtg aaaaatacgt aagtggtact gttgaagata
    21961 ttcgaatagc gacagaagag ctggggattt ttgcagaaat ctatggacgg ccaaaacaca
    22021 tttattcaat ctatcgtaaa atgaaagatc agaaaaagca gttcaacgaa atttatgact
    22081 tgctagctat tcgagtgatc gtagattcaa tcaaggattg ttatgctgta ttaggagcaa
    22141 tccacacaaa atggaaacca atgcccggca gatttaagga ttatattgcc atgccaaaag
    22201 ctaatatgta ccaatctttg catactaccg tgatcggtcc ggcaggaaat ccggtagaaa
    22261 ttcaaatccg aacacaggaa atgcatgaaa tcgctgaatt cggggttgct gcacactggg
    22321 cctataaaga aggaaaaaat gaaaaagtag aaccagatgg tatgacgaaa caattaagct
    22381 ggttccatga gatactcgaa cttcaagacg aaagctatga tgcttctgaa tttatggaag
    22441 gcgtaaaagg agatatcttt agtgataaag tctacgtctt cacaccaaaa ggagacgtta
    22501 ctgagttacc aaaaggatcc ggaccattgg actttgcata cagtatccat acagatatcg
    22561 gtaacaaaac cactggtgca aaagtaaatg gcaaaatggt gcagcttgat tacaaattga
    22621 aaaacggaga tatcattgag atcatgactt ctccaaattc atttggccca agtcgcgact
    22681 ggttgaaatt agttgctact agcaaagcaa gaaataagat caaacgtttc ttcaaagccc
    22741 aagatcgaga agaaaatgtg atcaaaggcc acgaatccgt ggtcaaatgt attacagatc
    22801 taggatttac gcctaaagat attttgacga agaacaaact gcaagaagca ctcgatcgtt
    22861 ttaattatca aacagaagat gatctctatg cagctgtagg gtatggagaa gttagcccct
    22921 tgacgatggc caatcgtctg actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa
    22981 agcaagaagc agaagaaatc atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac
    23041 gtcatgaagg tggcgttgtc attcaagggg tagaaaactt actaattcgt atcagtcgct
    23101 gctgtaatcc gattcctggt gatgatatcg ttggttatat cactaaaggc agagggatat
    23161 ccattcatcg tcgagattgt ccgaatgttc agcctgacaa accaaatgta gcagaacgtt
    23221 tgattgaagt cgaatgggaa gatacatcga atacacgaaa agagtatgat gcagatttgg
    23281 aaatttacgg ctataatcgt tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc
    23341 taacgaaaaa tctcaacagc gttgaagcac ggacgaataa agataaaatg gcgacgatcc
    23401 atttgacggt tggtatccag aatttatccc acctaaagag tatcgtggat aaaatcaaag
    23461 cagtacctga tgtctacagt gtacgccgga cgaatggata ggaggacaga tatgagagca
    23521 gttgttcaac gggtaagtaa agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt
    23581 ggctacggcc tacttgtttt attaggcatt catgaaaccg atacgcaaaa agatgtggac
    23641 tatctggtaa aaaaaattgc acaaatgcgt atttttgaag atgaacaagg aaaaatgaat
    23701 attagtgtag aagatgtggg aggacaaatt ttaagtatct ctcaattcac actgtttgcg
    23761 gatacgaaaa aaggaaatcg accaagcttt gtggaagccg cacgaccaga aacagctatt
    23821 cctttatacg aatcattcaa tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga
    23881 caattcggag cagatatgtc ggtttctctc gtcaatgatg gacctgtaac gatcattatt
    23941 gattctaaaa acaaataaag taatctggtt ttcactagta aaaactatga aacacgtcgc
    24001 ttaagttaga ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt
    24061 ttgtcagcag tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt
    24121 tgctgaagat gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt
    24181 atcaaaaagg atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca
    24241 aagcaaagat tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat
    24301 tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac
    24361 tttttggtca aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata
    24421 tttttatgga tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga
    24481 atctgacaca agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga
    24541 tggttttctt tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag
    24601 atgaggtcga aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc
    24661 cgattgatca cataaaaggt gtcgagaatc gcaaaaaatc agaaaaatgg gcgcaaggct
    24721 tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt
    24781 ttgccaatgc cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat
    24841 tattgaacat catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg
    24901 cacttttcag tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag
    24961 atccgtttat tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt
    25021 cgctgaaaga agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag
    25081 atttatatgg aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca
    25141 aacggtatgt agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac
    25201 aaggcggggg aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata
    25261 gtctacgtgt gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag
    25321 attggacgat tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca
    25381 agaattagat tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct
    25441 tttttcatat tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc
    25501 aatggtttga taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac
    25561 ttgctctgga cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca
    25621 agctaaaaca agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc
    25681 gacagcggtt aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg
    25741 atctccttca ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga
    25801 agcaagagga aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc
    25861 tttttcagcc tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc
    25921 tgcattcgct tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt
    25981 tcctgcttct tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc
    26041 cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc
    26101 caccgattgt ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg
    26161 agcagctttt gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc
    26221 gccaatcagt tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat
    26281 cccgctattc aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa
    26341 aagattatgt cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga
    26401 atgtccgatt cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa
    26461 taccaaaaag ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt
    26521 tgagactggt ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt
    26581 ttttgaaact ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc
    26641 taaagacact ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa
    26701 gaggaaagag aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt
    26761 tcatgatgaa caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg
    26821 agtttccaaa gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag
    26881 tttgtcgaag aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca
    26941 ccgatttttg aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt
    27001 tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa
    27061 ggtacagcac caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg
    27121 aaaccgttca aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga
    27181 cggttacgtc aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact
    27241 gatgtggaga gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc
    27301 cggctagtca tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg
    27361 atcgactatc ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat
    27421 gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa
    27481 gctccatcta tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt
    27541 tccatgctgg atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta
    27601 gattattaca cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa
    27661 gcaacgattt gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca
    27721 acacctggct tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag
    27781 gaagtagtta tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa
    27841 acaaatatcg aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac
    27901 cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc
    27961 aaattagtat tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca
    28021 atggcaagcc gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa
    28081 gtctatgatg aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga
    28141 tctattgcgg attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg
    28201 tacaaaaaag acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa
    28261 tcgttcaaat cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat
    28321 gtcgcagtga atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag
    28381 taaatcctaa aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga
    28441 atcaagcaaa aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac
    28501 gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa
    28561 gaaatgcgac aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg
    28621 aaacacctta cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac
    28681 gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc
    28741 tgatgaatgc tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt
    28801 tacgtggaga ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag
    28861 cagaagaaat ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg
    28921 gtatcgaagt cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg
    28981 gcagcgacaa accggataca cgttttgata tggaattgat cgatctaagc gatacagtca
    29041 aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat
    29101 tgaatgctaa aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat
    29161 atgtaggaca attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga
    29221 aaggtccaat tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg
    29281 caaaaccagg tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat
    29341 taggtgctat ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca
    29401 atttcttatg ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg
    29461 tctctgctca ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag
    29521 accctgcaaa agtctatgca gaagcttatg atatcgtctt gaacggttat gaattaggcg
    29581 gaggttctat ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct
    29641 ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat
    29701 tccctccaca tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag
    29761 aagataatat tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga
    29821 gtgatgcacc aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga
    29881 atatagaaga atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca
    29941 accttgattt aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt
    30001 ccatgaaaaa aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat
    30061 ctatcgttta tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata
    30121 tttattcaag tggtattact ggtctagctc agattttgac gactttgtca aaatcactga
    30181 taggattcga agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat
    30241 tggcgtggtt aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga
    30301 cctccctttg tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg
    30361 cgttgtttgg gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat
    30421 catcgggcgg tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg
    30481 gttctatttc gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct
    30541 ggcaatatat gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg
    30601 tttatactaa acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag
    30661 taaatgaaat ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg
    30721 cttataagca tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc
    30781 tacttagaga agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg
    30841 tccagatact tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat
    30901 gaaatactgc tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc
    30961 agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta
    31021 tttttttaaa tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg
    31081 ctattttcac ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat
    31141 tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc
    31201 cgttcattag ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc
    31261 ggctatttgg gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg
    31321 cggcaggaat gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg
    31381 aagagactct atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg
    31441 tttcagcgag ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc
    31501 aacaactggt ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca
    31561 ccgtttacgc atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc
    31621 ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta
    31681 ggtacatcct tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca
    31741 acccgtgttt attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt
    31801 gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt
    31861 ctgaatatcg ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat
    31921 gatcattaca atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg
    31981 ctagttgcag tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta
    32041 tttacttgga ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt
    32101 ttttatgaag caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa
    32161 atcacattga ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca
    32221 cgttttgatt atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc
    32281 agtaagagca aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt
    32341 ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc
    32401 gctgaaagta aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg
    32461 gttgaccaag cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa
    32521 aaattagaac taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa
    32581 gaactttatc caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta
    32641 ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag acactgttct gattggacta
    32701 ggaacgaatg gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat
    32761 cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat
    32821 gcgatgcttg aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc
    32881 tacagtaatg accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg
    32941 atgacacatt acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac
    33001 agcagtgaga caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt
    33061 ttttgcctga agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc
    33121 aaaacggttg agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat
    33181 gcagcttctg cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta
    33241 acaggtcttc ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct
    33301 aatcgtctag aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat
    33361 acgtaaaaat aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc
    33421 atccatattg taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt
    33481 gattccttca actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat
    33541 ccttttaggt gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg
    33601 acgatggacc atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac
    33661 ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt
    33721 tttatttgtt ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt
    33781 atgtcgaacg acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt
    33841 taaatggtta agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat
    33901 agcgtcgata gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc
    33961 acaaaggtca acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc
    34021 atctaaagaa aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa
    34081 ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg
    34141 tctgtattat actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt
    34201 atgcatacta tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg
    34261 gggtagttcg agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag
    34321 ttttaccgca agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat
    34381 tatatctggc ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt
    34441 tcaaaaatcc agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag
    34501 aaaatccagg accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga
    34561 gtttgatcat gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat
    34621 tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac
    34681 ttggggatct gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat
    34741 cagatatcaa gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag
    34801 cttttgacaa attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat
    34861 aaaatgataa gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact
    34921 ttttcaatga aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta
    34981 aaatagaaga caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt
    35041 ttgaggtgat tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca
    35101 aggttatgac ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa
    35161 agaagaaaaa gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc
    35221 tgttgatgcc gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat
    35281 cccgattttt gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc
    35341 atggttcagt tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt
    35401 cgagtttaaa aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt
    35461 atattttgaa gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa
    35521 aatgcaggag ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat
    35581 gggaagtgta aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc
    35641 tggtgaacta cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc
    35701 tttgagacag gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat
    35761 caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag
    35821 ggagatcgaa cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga
    35881 tcctgccttt tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag
    35941 aacgatcatc ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat
    36001 tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa
    36061 agatcctcaa aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc
    36121 aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag
    36181 aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga
    36241 tttttcacgt ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa
    36301 aacgacgatt tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg
    36361 ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt
    36421 ttcttttgaa catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc
    36481 aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga
    36541 tgggaaagga aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag
    36601 agaactgatg aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga
    36661 attccgtaat tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt
    36721 cggtactcaa tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca
    36781 acaaaaattg gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt
    36841 caaagaagag gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca
    36901 ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa
    36961 acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga
    37021 aaaaacgata ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga
    37081 aaagaaacga tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg
    37141 cctacaagaa ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa
    37201 gaaactagcg atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt
    37261 acgccgacaa acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt
    37321 gccaattgtc gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt
    37381 ggataaagaa gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag
    37441 aactcgaatt cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc
    37501 ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa
    37561 agacactggg tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac
    37621 aaaaaatagt cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg
    37681 ggcaaagcag caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt
    37741 ttgtggatca acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga
    37801 tatcttaaaa gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca
    37861 aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga
    37921 acaaaaaatc atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc
    37981 tttgaaaaag gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat
    38041 cgaacaatct ttagatagac gaaaaagcaa gatggccaat gcaagaatag agctggaaga
    38101 aataagacaa caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac
    38161 agaaaaaaat aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt
    38221 gcaagatacg gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga
    38281 agaagactcg aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct
    38341 tcatacgctt cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa
    38401 aatagaaaag gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac
    38461 aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac
    38521 agttacttct tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa
    38581 tatccagcta actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat
    38641 aagacgtatg atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag
    38701 taatcaaaaa atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga
    38761 tgagcttacg caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac
    38821 tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga
    38881 tcatctccaa cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag
    38941 ttatcgaggg aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg
    39001 aagtgcgcat accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc
    39061 tcttgccgat gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga
    39121 tgaaggattt gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac
    39181 gatcgaaagt gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg
    39241 agtattacaa caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca
    39301 acttggataa aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc
    39361 acgtggacgt caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga
    39421 aaacgtgtgg catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac
    39481 agcaaaagag atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca
    39541 tacagttgct gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga
    39601 ccagccaatg ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt
    39661 ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca
    39721 agtagaaacg attgagacta atcagtatca cccagagctt tctattttgg cactttactt
    39781 gcgtatcggt tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt
    39841 tttacaagct tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct
    39901 agataaacgg gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc
    39961 tcagacgcat caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag
    40021 atatctgctt ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac
    40081 acgtttgcat ctgcagataa atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa
    40141 caaaccttta tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa
    40201 tgattttgat tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc
    40261 tctgacgaaa tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga
    40321 gaaaccagaa gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc
    40381 tctgctgtca gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga
    40441 tatccccgtc gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt
    40501 cgatttcgtc tatggtgaag tcatctattc gactgacccg aagcatgagg tctcgacaga
    40561 acaggctttt caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag
    40621 aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga
    40681 actatatgcc tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact
    40741 tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt
    40801 aaccgagacc ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga
    40861 aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg
    40921 tcaggttctt tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt
    40981 acgggaatcg atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat
    41041 cattcaaaat caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt
    41101 acaagatctc attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac
    41161 gatgagagat tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt
    41221 tggcggtatt ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt
    41281 actttcggaa aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt
    41341 aatttacaac tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat
    41401 caatgggaat aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac
    41461 ttcttatgcc agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt
    41521 gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag
    41581 agaattagct gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga
    41641 agaattgtgg tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt
    41701 cagggaactt gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga
    41761 taaacaaact gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact
    41821 tacagaagaa caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac
    41881 atcaatggat tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag
    41941 actgcgacaa atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg
    42001 caagctcgaa caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt
    42061 gctgttttct caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg
    42121 gatctcgaca ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga
    42181 cgcatttaat gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg
    42241 attgaatctc acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat
    42301 tgaagaacaa gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg
    42361 gcgtatgatc tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg
    42421 cgaactgttc caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga
    42481 agacatccgc atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt
    42541 tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag
    42601 caaggaggct gggattccat tttatcccag cttccttgct atcatttttt tatttctatt
    42661 tttaaaggtt atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc
    42721 tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct
    42781 tgccagttag cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag
    42841 gtctcttctg gaagcatatg aaattctttc tcagccagct gaaaaatctc tttttctggt
    42901 ttttggaaac ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat
    42961 tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat
    43021 gggacattct tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata
    43081 ttatcaagtt cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga
    43141 gggtagtcta aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc
    43201 caatctcctg ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat
    43261 agtggatgca tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat
    43321 ggctgctgct gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt
    43381 ttacacgcct ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa
    43441 aattgtctaa aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca
    43501 gaataaggtt tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt
    43561 tgatgaacaa attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt
    43621 ggaattttcg aaagatttat tttt
//
LOCUS       DACCWV010000007        42772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCWV010000007 DACCWV010000000
VERSION     DACCWV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.007"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(84..461)
                     /locus_tag="IVW84_000384"
     CDS             complement(84..461)
                     /locus_tag="IVW84_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916611.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            717..1445
                     /locus_tag="IVW84_000385"
     CDS             717..1445
                     /locus_tag="IVW84_000385"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7916612.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1445..1699
                     /gene="purS"
                     /locus_tag="IVW84_000386"
     CDS             1445..1699
                     /gene="purS"
                     /locus_tag="IVW84_000386"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7916613.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1701..2372
                     /gene="purQ"
                     /locus_tag="IVW84_000387"
     CDS             1701..2372
                     /gene="purQ"
                     /locus_tag="IVW84_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7916614.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2373..4595
                     /gene="purL"
                     /locus_tag="IVW84_000388"
     CDS             2373..4595
                     /gene="purL"
                     /locus_tag="IVW84_000388"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7916615.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4580..6019
                     /locus_tag="IVW84_000389"
     CDS             4580..6019
                     /locus_tag="IVW84_000389"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7916616.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6051..7094
                     /locus_tag="IVW84_000390"
     CDS             6051..7094
                     /locus_tag="IVW84_000390"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7916617.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7091..7669
                     /gene="purN"
                     /locus_tag="IVW84_000391"
     CDS             7091..7669
                     /gene="purN"
                     /locus_tag="IVW84_000391"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7916618.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7666..9201
                     /gene="purH"
                     /locus_tag="IVW84_000392"
     CDS             7666..9201
                     /gene="purH"
                     /locus_tag="IVW84_000392"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7916619.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9217..10491
                     /gene="purD"
                     /locus_tag="IVW84_000393"
     CDS             9217..10491
                     /gene="purD"
                     /locus_tag="IVW84_000393"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7916620.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10613..11527
                     /locus_tag="IVW84_000394"
     CDS             10613..11527
                     /locus_tag="IVW84_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7916621.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11660..13225)
                     /locus_tag="IVW84_000395"
     CDS             complement(11660..13225)
                     /locus_tag="IVW84_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7916622.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13218..14669)
                     /locus_tag="IVW84_000396"
     CDS             complement(13218..14669)
                     /locus_tag="IVW84_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7916623.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14683..15699)
                     /locus_tag="IVW84_000397"
     CDS             complement(14683..15699)
                     /locus_tag="IVW84_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7916624.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15919..17007
                     /locus_tag="IVW84_000398"
     CDS             15919..17007
                     /locus_tag="IVW84_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7916625.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17061..18587
                     /locus_tag="IVW84_000399"
     CDS             17061..18587
                     /locus_tag="IVW84_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7916626.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18589..19794
                     /locus_tag="IVW84_000400"
     CDS             18589..19794
                     /locus_tag="IVW84_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916627.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20051..21028
                     /gene="guaC"
                     /locus_tag="IVW84_000401"
     CDS             20051..21028
                     /gene="guaC"
                     /locus_tag="IVW84_000401"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7916628.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21292..22716
                     /locus_tag="IVW84_000402"
     CDS             21292..22716
                     /locus_tag="IVW84_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7916629.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23177..24286
                     /gene="pdhA"
                     /locus_tag="IVW84_000403"
     CDS             23177..24286
                     /gene="pdhA"
                     /locus_tag="IVW84_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7916630.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24289..25266
                     /locus_tag="IVW84_000404"
     CDS             24289..25266
                     /locus_tag="IVW84_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7916631.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25291..26934
                     /locus_tag="IVW84_000405"
     CDS             25291..26934
                     /locus_tag="IVW84_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP7916632.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26942..28348
                     /gene="lpdA"
                     /locus_tag="IVW84_000406"
     CDS             26942..28348
                     /gene="lpdA"
                     /locus_tag="IVW84_000406"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7916633.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28510..28785
                     /locus_tag="IVW84_000407"
     CDS             28510..28785
                     /locus_tag="IVW84_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7916634.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28791..29558
                     /locus_tag="IVW84_000408"
     CDS             28791..29558
                     /locus_tag="IVW84_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7916635.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29726..31555
                     /gene="typA"
                     /locus_tag="IVW84_000409"
     CDS             29726..31555
                     /gene="typA"
                     /locus_tag="IVW84_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7916636.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31816..33588
                     /gene="recQ"
                     /locus_tag="IVW84_000410"
     CDS             31816..33588
                     /gene="recQ"
                     /locus_tag="IVW84_000410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7916637.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33702..34004
                     /locus_tag="IVW84_000411"
     CDS             33702..34004
                     /locus_tag="IVW84_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7916638.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34007..35170
                     /locus_tag="IVW84_000412"
     CDS             34007..35170
                     /locus_tag="IVW84_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7916639.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35196..38624
                     /locus_tag="IVW84_000413"
     CDS             35196..38624
                     /locus_tag="IVW84_000413"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7916640.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38645..39769
                     /locus_tag="IVW84_000414"
     CDS             38645..39769
                     /locus_tag="IVW84_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916641.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39759..40196
                     /locus_tag="IVW84_000415"
     CDS             39759..40196
                     /locus_tag="IVW84_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7916642.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40180..40518
                     /locus_tag="IVW84_000416"
     CDS             40180..40518
                     /locus_tag="IVW84_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7916643.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40637..41197
                     /gene="rsmD"
                     /locus_tag="IVW84_000417"
     CDS             40637..41197
                     /gene="rsmD"
                     /locus_tag="IVW84_000417"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7916644.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41194..41685
                     /gene="coaD"
                     /locus_tag="IVW84_000418"
     CDS             41194..41685
                     /gene="coaD"
                     /locus_tag="IVW84_000418"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7916645.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41675..42718
                     /locus_tag="IVW84_000419"
     CDS             41675..42718
                     /locus_tag="IVW84_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7916646.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        14109 a         7335 c         9554 g        11774 t
ORIGIN      
        1 tattttctta tcaataatag gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc
       61 tttacataca aaaaatagag gaattaagcg taaatggctt ggctattata actaaaatgc
      121 tggttatgga gatgttgcag caagttttcg agaaaagcgt attccatctc ttgtccattg
      181 aataagatct ggatacgata atgatttttg aaacgcttct gttcatgaat ctgactgatt
      241 gaaagttcgc ctttgtgttc tgtctgataa agaaaagtcc ttttacggca ttcagcacag
      301 cgcttggttg ctcggcggat ttttcggaat gtataaaaac tagtatcttt attgcataaa
      361 taaacgtctt tgatcgacag caaatcatta tatagacagg aacgttcttc agctgttaat
      421 tctgataaaa aaagctgacc ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa
      481 cccattatcc aaatttttag ttaattctaa tataccatca ttaagtataa atgtaaacgc
      541 ttgttgatgg tataagattt tcgtttctga taaaacacga acattaaaag aatctaatgc
      601 cttaaatgta catatttttg ttgatttata aaaagatgat tgctaaaatt taaagtgaaa
      661 aaacgaacaa aaaacgatgt atttgctttt aatgtaaata aaaaggagag acgtacatgg
      721 agaagaaaga acttttgtat gaaggaaaag ccaaaaaaat atatcttact tctgatgaga
      781 acttgatttg gatagaatat ttagatcaag caactgcgtt gaatggcgcg aggaaagacc
      841 agataagtgg aaaaggagaa ttgaataatc aaatcacaag tgcaattttc cgttatctga
      901 ctcaaagagg aataaaaaat cattttattg aacaattgtc taaaaccgaa caattagcaa
      961 aaaaagtaca aattatccct ctggaagtgg ttatccgaaa tattgcagca ggaagtttct
     1021 caaaacgtct aggtgttccc gaaggtaaag aattcatcaa accaatcatt gaattctact
     1081 ataaggatga ccagttggat gatccattta tcaatgaaga gcatatctat ttcttacaac
     1141 ttgcaaagcc ggaagaaatc agtgagctga aagcccaagc gttggctatt aatgaaattt
     1201 tgaccgaatt atttgctaag gtcggtttgg ttttagtaga tttcaagcta gaatttggcc
     1261 ggtcgaatgg acaaattttg cttgcggatg aagtaacacc ggatacttgc cgattatggg
     1321 atgaacgtac gaatgaacat atggacaaag acgtttaccg cagagatcta ggtgatttga
     1381 tcccagtcta tcaagaagta tataaccgtc tatcggaact aactgaagaa aaggagcgag
     1441 tatgatgtat ttcgtaaaag tatatgtaac ctataaagat tcagtgttag atcctcaagg
     1501 ggaggcagta aaaaaagcag tccatcgtat gggatatgaa acaatcgaag atatccgaat
     1561 aggaaaatac tttgagatac aacttgctga tgcagaagaa ccaatcgata cagtgattga
     1621 aacgatttgt gacaagctgc ttgcaaatgt aaatatggaa acataccgat atgaaatcaa
     1681 aaaaatggag gaagtataac atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg
     1741 acgcagatat gttatgggca gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc
     1801 atgatgagtc ctctttagaa ggatttgatg gggttttact gccgggagga ttctcctatg
     1861 gtgactattt gagatgcggt gcgattgctc ggttttctac aatcatggaa gaagtgattc
     1921 gttttgctga agaagggaaa ccagtattcg gtacatgcaa tggattccag atattgacag
     1981 aagcaggact tttgccaggt gtactgagac gaaatgagtc tttgcatttt atttgcaaaa
     2041 ctgtaccatt gaaagtcaac cccaagacgg cctttacttc agagtatgaa gccgacgaaa
     2101 taatctatct tccagttgct catggggaag gaaattattt ctgtgacgaa gaaacacttc
     2161 aagaactaaa aaaaaataat cagattgcct ttacttataa tgacaacatc aacggcagcg
     2221 tggaaaacat tgcagggatt acgaacaaag cagggaatgt gttaggaatg atgcctcatc
     2281 cagaacgtgc agtagaagac ttgctaggat cagaagatgg gaaacggttc tttgcttcca
     2341 ttttaaaaaa cttcggaaag gtgaccgtat agatgaaaac attgaaagaa ccgacagctg
     2401 aagagatcaa gaaaaaaaga atctatgccg aatggggatt gactgatgaa gaatatcgaa
     2461 tgatcgaaga agatatctta ggaagaatgc ctaattatac ggaaactgga ctgttttctg
     2521 ttatgtggag cgaacattgt tcctataaaa attctaaacc ggttctacgc aaatttccta
     2581 ctagtggacc tcatgtgttg caaggccctg gagaaggcgc aggcatcgtc gacatcggtg
     2641 atggactagc agttgtcttc aaagcagaaa gtcacaatca tccttcagcc gtggagcctt
     2701 atgaaggagc tgcgacagga gttggcggaa tcatcagaga tattttcagc atgggggctc
     2761 gtccaattgc gttgctagat tcattgaggt tcggcgaatt agataatcca agaacgaaat
     2821 atttactaga agaagtagtt gcaggtatcg gcggatacgg caattgtatc ggcataccga
     2881 ctgtaggcgg cgaaattgca tttgatcctt gttatgaagg aaacccatta gtcaatgcaa
     2941 tgtgcgtagg tttgattgaa cacaaacata tccagaaagg gcaagccgct ggcgtcggta
     3001 attcgatcat gtatgtaggt gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg
     3061 cctcagaaga atttaatcaa gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc
     3121 cttttatgga aaagctgctg cttgaagcgt gtttggaatt aatcttgcat cattcggatt
     3181 ctctaatagg tatacaggat atgggagcag caggacttgt ttcatctagt gcggaaatgg
     3241 cttcaaaagc tggctcagga cttatattga cattagatga agtcccacaa agagaaactg
     3301 ggatgacacc ttatgaaatg atgttatcgg aatcacaaga acggatgttg atttgtgtta
     3361 aatcaggaga ggaagaaaaa atccaagaac tatttcagaa gtatgatcta gatgcagtca
     3421 ctatcggtaa agtaacagac gacgggcaat atcggctata tcatcaagga gaagaagtag
     3481 caaatctgcc agtcgatgca ttagctgaag atgcaccagt ttatcataaa gaaatgaaag
     3541 aaccagctcg aatcgtagaa tttcagcagt tagctcctta tcagccagtt atcgaagaac
     3601 ctggacaagt attgcttgat ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct
     3661 acgaaactta tgattcacaa gtacaaacca atacagttgt ccgtccagga agcgatgctg
     3721 ctgtgatgag ggttagaggg acgaataaag cgttagctat gaccacagac tgcaacgcgc
     3781 gctatctgta tttggatcca aaaatcggag gacaaatagc agtggcagaa gctgctagaa
     3841 atattattgc tagcggtggt aagcccttgg cgatcaccga ctgcctgaac tatggttctc
     3901 ctgataaacc agaagttttt tgggaattat cgacttcagc tgacggaatt gctgctgcgt
     3961 gtgaaacgtt aggaactcct gttatttcag gaaatgtctc tttatataat gaaacggatg
     4021 gtcaagcaat ctatccaaca ccaatgattg gtatggtcgg attgattgag gatcataaac
     4081 atatcactac tcaggaattc aaaaaatcag gggatctaat ctatatatta ggtaagacgt
     4141 ttgcagattt tgatggcagt gaattacaaa agatgcagct tggaagaatc gaaggtgtga
     4201 tccgaaactt tgatctatcc attgaaaaga gaaaccaaga gttagtattg acagccatcc
     4261 aaaacggatt gattgaaagt gctcatgact gttcagaagg tggcttggca attgcactgg
     4321 cagaatcagc tttcaaacat cagctaggga tatctgtaca gtttgaattg tcctctgctc
     4381 aattatttgc agaaactcaa tcaagatttg tgttgacagt cgcaccggaa aataagacac
     4441 gattcgaaga aatgatggga gatgctgctg ttttagcagg taaggtgacg gatgaagcga
     4501 taatcgaaat ttcagcaact gacggtcaga tcaaaataga aactgcagta gcaagaaagt
     4561 gctgggagga tgcaatcgta tgtcttatga agtgaagagt ttaaatgaag aatgcgggat
     4621 cttcggtgtt tggggtcatc cagacgccgc tagtgtggca tacttcgggc tccatagtct
     4681 tcagcatcga ggacaagaag gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg
     4741 gcatcgagat ttggggctgc tttctgaggt attcaaagat caaagaaaat tgcagcagtt
     4801 aacaggagaa gctgcaatcg gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga
     4861 caatattcag ccttttctat tcaaatttca tgatcagtcg atcggcttag cgcataatgg
     4921 taacttgatc aatgctcgtt cccttagaaa agagttagag gaaaatgggg cgatcttcca
     4981 ttctaactcc gatacggaga tattgatgca tttgatccga cgaagtgaag aaacagattt
     5041 tttaaataaa ttaaaaagtg ctttgaatga agtcaaaggt ggctttgcct atttattaat
     5101 gacagaaacg gcaatgattg ccgctttgga cccaaatggt tttcgtccat tagcaatcgg
     5161 acaaatgaaa aacggagcat acgtgattgc ctcggaaact tgtgcattag aagttattgg
     5221 ggcacgtttt ataagagatg tccagcctgg tgagatagtg atcatcaatg acgaaggaat
     5281 ccagattgat tgctttacga aggacacgca gctttctatt tgttcgatgg aatatatcta
     5341 ttttgccaga ccagattcaa acatcgcagg gatcaatgtg catactgcac ggaaaaatat
     5401 gggacggaat ttagcgaaag aatcgccagt aaaagcagat atggtcgttg gcgtacctaa
     5461 ttcttctttg tcagccgcaa gcggttatgc ggaagctagt ggaatccctt atgaaatcgg
     5521 attagtaaaa aatcaataca ttgcccgaac atttatccag ccaacgcctg agctaagaga
     5581 gcaaggtgtt cgaatgaaat tatctgctgt acgaggagta gtggaaggta aaaaggtgat
     5641 tatggtcgat gattcaattg ttcggggtac gacaagcaga cggatcgtcc atcttttgaa
     5701 agaagcaggg gcaaaagaag tccatgtgag gattgcttct cctcctttga aatatccatg
     5761 tttttacggg attgatattc aaacgcgtaa agaactcatt gcggcaaatc acacgattga
     5821 tgaaatcaaa gaatgtatcg gtgcagattc tttaggtttt ttaagtgaag agggtctgat
     5881 cgaatcgatc ggtctggaaa aagatgctcc gtattcagga ttgtgtatgg cttatttcaa
     5941 cggagattat ccgacgccgt tatatgacta tgaagaaaaa tatcaggaat cactaaaaga
     6001 aaaagtatcg ttcttttaaa agcagaccaa gaagggagta tatgaattca atggcaaatg
     6061 catacgcaaa agctggcgta gatgtcgaag ctggatatga agtagtagaa cggatcaaga
     6121 agcatgtcaa aaaaacggaa cgtttaggta tgatgggcgc tataggagga ttcggcggtt
     6181 gttttgattt atcggtcttt caattaaaag agccagtcct tgtctcgggg accgatggcg
     6241 tggggacgaa attagcagtg gcaatcgaag cagacaaaca cgatacaatc gggatcgact
     6301 gcgtagcgat gtgtgtcaat gatatcttag cacaaggcgc agagccgtta tatttcttag
     6361 actacttagc aacggggaaa aatgatccgt tacgtttgga aaatgtagta aaaggtgtag
     6421 cagatggatg tgttcaagga aatatggcgc tgattggcgg agaaacagct gaaatgcctg
     6481 gtatgtactc aggtaatgat tatgatctag ccggatttgc tgtagggata gctgaaaaat
     6541 ctcaattatt gacaggagaa acgatcaacc caggagatgt gttgcttgga atagcatcta
     6601 gcgggattca ttcaaatggg tactctcttg ttcgcaaaat tttctttgag cagcagcagt
     6661 tttctttatc atcaaaactg ccagaattga aaaaaacact tggcgaagaa ttgcttgagc
     6721 caacgaaaat ctatgtttct tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag
     6781 ctgcacatat cactggtgga gggtttgtag aaaatatccc gcgaatgtta ccagaatcta
     6841 tggctgcaca aatcaaactt ggctcatggc cgattcttcc aatattcggc tgtttacaaa
     6901 aatatggtga aattcctgag atggagatgt atgaaatctt caatatgggc atcggtatgg
     6961 tcctggcagt cgaagaatcg aaagtaaaag aaatagtaca cgaaattgag caagctggcg
     7021 aaaaagttta tgaaatcggg aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag
     7081 gagaagaacg atgagaatcg ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct
     7141 agccgattat ctatcgaaaa aaggattgga atcttcgatt gactggttgt tttgtgatca
     7201 gcctgaagca tatgttttga aacgtgcaac tgcgttgagt gtaccggcag attgtttttc
     7261 acctaaagaa tttgattcaa aaaaagagta tgaagaagca attttgcata aattgaaaga
     7321 aaaaaagatc gatttgatcg tattagctgg atatatgcga atcattggac cggtattgct
     7381 gaaaaattat gataaaagaa tcatcaatat ccatccgtct cttttacctg ccttccctgg
     7441 tttgcatggc atacgtgatg cctttgaagc tggagtaaaa gaaacgggtg tcacaattca
     7501 ttatatcgac caaggtgtag atactggacc aatcatccgg caggaaaaag tgagaattga
     7561 acaggaggac acgttcgatt ctttagaaga aaaaatccat agagtagagc atcgaatcta
     7621 tccagaagtg attagtgaaa ttattgaaaa tggaggctat ctgaaatgac aagagcattg
     7681 atcagtgtat cagataaaac tgggattgta gcattcgccc aaggtttgat tgaacaagga
     7741 gtagaaatca tttcaacagg aggaacaaag catgcgttgg ataaagctgg gatcaaaacg
     7801 ctttcgatcg aagaagtgac agggtttcct gaaatgatgg atggccgtgt caaaacactt
     7861 catcctaaaa tccatggtgg tcttttagga agacgcgatc tccctagcca tatggaggca
     7921 atggaaacgg aaaatatcca accaatcgac tttgtttgtg tcaatttata tcctttcaaa
     7981 gaaacgatta tgaaaccaga tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt
     8041 ggtcctagta tgctccgtag tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat
     8101 ccatctgact atgaagaagt actttcagaa ttacgtcagt atgggcatac ttcactttct
     8161 ttcagacaaa aactagcggc aaaagtcttt cgtcatactg cggcatatga tgcactgatc
     8221 gctcaatact taacggattg ggtggaagaa aaagaaccgg aaaaattgac acttacgtat
     8281 gaacgaaaac aaacgcttcg ttacggtgaa aatagccatc aagccgctgc cttttatcag
     8341 tccgctcttc tggaaaaata ttcgatcgca gcagcaaaac aactccatgg gaaagaactt
     8401 tcttataata atatccgaga tgcagatgct gcgctacgga tcatgaagga atatgacgag
     8461 ccaacggtag tcgccttgaa acacatgaat ccttgtggta ttgggacagc atctacgatt
     8521 ttagaagcgt gggaagcagc gtatgaagca gatcctgttt caatctttgg tgggatcatc
     8581 gttttgaatc gagaagtaga tctgccaaca gcagaacaga tgcataagct gtttctagaa
     8641 atcatcattg ccccttctta tacacaagaa gctttggaat tactgcaaaa aaagaaaaac
     8701 atccgattac tgacagtaga ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc
     8761 tcagtcttag gtggacttct tgtacaagat caagatctgg caatagaaaa tgaagaagag
     8821 tggaaagtcg tgacaaaacg taagccgact ccagcagaag caaaagcatt agtgtttgct
     8881 tggagagctg tcaaacatgt gaaatctaat gcgatcgtac tggcaaacga acgtcagaca
     8941 gtaggaatcg gagccgggca gatgaatcga gtcggttcag taaaaattgc gattgaacaa
     9001 gcgcaagaga aaatggaagg tgcagtttta gctagcgatg cttattttcc aatggatgat
     9061 tcggttgaat atgcagccaa acatggtata aaagcaatca ttcagccagg aggaagcatc
     9121 aaagaccaag attctattga tatggcgaat aaatacgatc tagcaatggt ctttactgat
     9181 gttcggcatt ttagacatta gagaggagtc ggataaatga acattttggt gatcggtgca
     9241 ggtggtcgtg aacatgcaat aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg
     9301 tattgtgcgc caggaaatcc ggggatgaca aaagatggta tcaattgtgt cgctatcagt
     9361 gagcgagatc attcagcgtt aatccatttt gttaagaaag aggctattga ttggagtatc
     9421 gttggtcctg aagttccttt gttaaatggg atcgtcgatg attttatggc ggaaggattg
     9481 aaaatcttcg gtccgacaaa agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa
     9541 caattaatgg atacttatca aatcccgaca gctaaatcca gaagttttga atgttacgaa
     9601 aaagcagcag cgtatatcaa agaaaatggt gcgccaatcg tagtgaaagc ggacggctta
     9661 gcagctggga aaggtgtagt cgtggctacc acagtgaagg aagcgctgga tgcggcagaa
     9721 caaatgttga accagcatcg atttggggat agtagcaaaa aaatcgtcgt agaagagttt
     9781 ttggcaggag aagaattctc ccttttggcg tttgtccgta ataccaaggt gtatccgatg
     9841 gtcatttcgc aagatcataa gcgggcattt gataacgata aagggccaaa tacaggcgga
     9901 atgggtgctt attcaccagt accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa
     9961 gaagttttaa aaccagcggc tttgggaatg aaagaactag gacgctcttt cacgggtgtt
    10021 ttatatgcgg gtttgattgc tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt
    10081 ttcggtgatc ctgaaacgca agtcgttctt tctcggttga agagtgactt tgcgcaagta
    10141 atcgatgatt tattagagaa tcgtacagta gagttaaagt ggcagcaaga aggatacaat
    10201 gtaggcgttg tggttgcagc tgccggttac cccgaagaat atgagacagg aatggttcta
    10261 ccagattttc tggaagaaga gctttctgtt tactatgctg gagtttcctc aaataacggc
    10321 aaactagttg gagcaggagg acgtctttat ttagtagaag catacggtga gacgatcaaa
    10381 gaagcacaga aaaaaattta caccgcgtta atggataaaa cgacagatgg aacattttat
    10441 cgtacagata ttggagaaaa agctgccaat agcgaactga tcgtgaagta aatcagctgc
    10501 ttccatttat aaaaaataca acaaacataa tcggaagcgc cgattttagt gaagcctttt
    10561 cataaagagt gtggtacact tttgttgtgt atataaataa ggaggagttc agatgaaaaa
    10621 aattcaatta ggtacttctg atcagaaagc agctgcagtg atcttaggct gtatgcgtat
    10681 caacaatgcg gaagatccag taaaagtgat tgagacagct tatgaaaacg gcattgattt
    10741 ttttgaccat gcagatattt atggaaacgg tgaatgtgaa gaaatctttg cagatgctct
    10801 agcaaaaaca tcaatcaaac gagaagatct ttttattcaa acaaaatgtg gaatcgttcc
    10861 aggtgtgatg tttgattttt ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac
    10921 ccgtctgaaa atggattatg tggatgcttt acttcttcat cgtccagata cattggtaga
    10981 accagaggaa gtagctgaag cgtttgatga attggaacgg tcaggaaaag ttcgttattt
    11041 tggcgtcagc aatcaaaaac caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc
    11101 attagtagct aatcagctgc aatttggctt gaaacatact ggaatgatcg atcaagggat
    11161 tcatgtcaat atgacagatg aagcaagtat cgatcatgac ggtagtgtac ttgattattc
    11221 ccgattgcat gatatgacga tccaagcatg gtcaccatac cagtatggat tttttgaagg
    11281 tgtatttatc gggaacgaaa aattccctga attaaaccaa acattagaag aactagcagc
    11341 taaatatcaa acgacaccaa ctggcttggc ttctgcttgg attttacgcc atccagccaa
    11401 tatgcaagtg atcgccggta cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc
    11461 agaaattaat atgtctcggg aagattggta cgaattgtac cgtgctgctg gaaacatttt
    11521 accttaaaga ttgtcataag caggtcaaaa gggctataaa ttaaaaagac agaataactt
    11581 gaaagctgtt ttcagcttcg agatattctg tcttttttta ttgcaaatgg aaagagtatc
    11641 attgtacgga tttcgtttat tagttatttt tgtttttaag gaaaagctgt gtccgatatt
    11701 cttttggaga aatcccttgt tttttcttaa ataaataact gaaatagtta gggtcattat
    11761 aaccaatttc aaaagcaata tctgaaatac gatagtctga ttgctgcaat aatttttttg
    11821 ctaattcgat tctttgttct gtcagataat cgatgaatgt ttggttaaac gattgagaaa
    11881 aaatcgtact gaaatgagag ggacttaatg caacttgttg agcgacccaa gttaaggaca
    11941 tatctggatc ggtaaaattt tctttgataa aatccaatgc atggttcaaa attggctgat
    12001 atttgttcct tgaaggctgt gagggcagag cagttaagaa agaaatgaac atataaactg
    12061 tcgaacggta aagcgagtcg tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat
    12121 gaaccatttc ttgcgggatt ttcttttctt tcaggcagac atgctggagt agcttagtca
    12181 agacaaataa acgatataat cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt
    12241 cgatgtactt agatacttcg tttttcgata atgaagacaa atcaagtgag aggtcaaagt
    12301 aaaagacaga atcaactgga agtttcgctt cttgaatcaa atcctcatag ctgataattt
    12361 gttctttaag ggaactggcg gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta
    12421 attcgctgat tctttctgcg acaggaccaa tggcgacaga aaaattattt ttttgtccct
    12481 tttctaattc atggaccaac gtttgggcta cctgataact ggtctcgatc acattttctt
    12541 caagagaatc aactattaaa aatttgatga atcgagagga aacagcagag aagatcactt
    12601 gttgattctc cctaaataaa aacttcaaat aatcactgag atgagcgtaa tcttcaaagt
    12661 ctgtactgac ttggtttgct gctaataata cggtcatttt tttccctgct aaagagcgat
    12721 tgaaagattc agcaagtttc aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt
    12781 tcaagaaatg attttttttc aattcgaaaa caagatcaga agaagtgtgc gtttcttttt
    12841 gctgcaaatc taaagcagtt actgcttttt ctattgcttg tttcaattcg ctacttttaa
    12901 tcggtttaag cagataatca tctgcctgta cctggattgc tcctttcata taatcgaaat
    12961 cgtcgaatcc actaataaat atgagacgag tccaaggaag tagtttcctt gcttcttttg
    13021 aaaaagtcaa cccgtccatg aaaggcattt ggatatcagt cagaatgatg tcgggctgta
    13081 agtcgataat tgaagacagt gccatctcac catctccagc ttcgccgaca taagtgattg
    13141 gataataaga accaaggaca gttatttgtt cccgcaagtt attgcgaatg aggtattcgt
    13201 cttcgatgat aaaaacttta tacatgtttg tttcctccaa ttgttttggg agatagaggg
    13261 atcatcagtg tgacagttgt tccttctcgg tatttgctgt caatcgtgat aggtttttga
    13321 tcatttccgt aataaagaag gagacgcttg tttacgttgt aaagtccgta gccgatactg
    13381 aaatctgaat cgatcccttt ggctaactct ttacgtattt ctgccagttt ttctgcaggg
    13441 atgccaatcc cgttgtctat aactttgaat tggatcatct catcaatttt gcatgtggta
    13501 atacgaataa atcctttgtg acggacgtac ttcgttccat ggtatacagc attttcaatc
    13561 aatggctgca agatcatttt taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc
    13621 ttatagtcaa gcatatggcc gtatcttatt tttaagatga ctagataatc attgatatgg
    13681 ctaatttctt tttcaagagg aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag
    13741 ccggaaagtg catgggtcat agtcagagct tcttcaaagc agtcttgttc gatcaaagaa
    13801 ataatggcat ccaaactgtt ataaataaaa tggggagtaa tctgagcttg caatacacga
    13861 acttcacttt gtgctaaatg gtattgtttc aaagcgttct cccgtaaaga ctggtttaga
    13921 tcttccgcca ttttattcat gctagcggta agtgtttgta attccagtgt ttccggcagg
    13981 gttaaacgaa agccaaggtt ccctgaagaa agttgttctg acatctcgat cagctgattg
    14041 atcggttctt ggacctttct ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg
    14101 gtactaagaa ctccaagttc cactattgtt aatagaatga gcgagcggac aagtgtcgta
    14161 ttttttttgc ttgctaagtc gatctccaca cgaacaaaat cttgtaagat atctgacagc
    14221 agttgagtga cagaatctac ctgattgatg atctcttcat tggtatccat aaaatctttt
    14281 gccaagatat tattgatcag ctcgttttga taatcttcca gtgtatcgat cgtacgtagc
    14341 gatacatcta acgtactaat ttctttaagt gtatttgtat tatttttgct ctctcgaata
    14401 tcattcctaa gttcttctag gatattttcc tcattgaact catctacggt tacttggcca
    14461 gaaatcaagt cccacatact ttccaaaact ttttgatcaa ccttcaaagc aattgcatct
    14521 gctttttgga tatttgcgat cgtctgttga taaatcaaaa gctgttgcga atataacagt
    14581 atgctcagta gcaaagggat aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa
    14641 atctgtttta tgcttaaatt ttttgtcatg atacttctcc agtcaataat ttctggttgg
    14701 gatgttagct atattctgct gggaaaagac agtctcggaa ataaaaatat ctttaggtaa
    14761 tgtttttcct gtttgaatat ttccaaaata acgagtaacg gtattggcta cataccagcc
    14821 gacatcaggt ttacactcga ctacacaatt gacgattcct gagcgtagct tctcgatcat
    14881 atcttcttgt ccatcaatag ttacaatgat caaatcttct ccaggtttga ttgatgtttc
    14941 ttttatcgca gctaaagcac cgtgagtcat ttcatcacta tggctgtata atacatcgat
    15001 ttcggataaa tcatttgttt ctaaatatcg tttcatctgg tcttttcctt tcatccgaat
    15061 aaaatcaccg acaattcggt gaacaatagt catcggaggt ttatggtcga tggttccatc
    15121 tttttttagg gttggtttac gattgattga ttccaaaaat ccttcggagc gtagcttggt
    15181 cggtgtggag ttctctgaac cagcaagttc taggattctg atttcctgtt tctttgaatt
    15241 tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct tctgctttga aactggggcc
    15301 aatgtgggtg acgaaaagct cgggatcatt cgtttgaata tgtctgtcga caagtatgac
    15361 agggatccct gctcgctttg cttcttttaa tacagagtcc cacccggatt cttgcaaagg
    15421 agtaaaaatg atcatatcca ctttataggc aatgaacgtc cgtatatctt ggatttgccg
    15481 ttcctgattc atgtagccat ttcggtagag aacttggtaa tcttcttttt caagcgcagt
    15541 gataattgat tcagtatgct ttttccgcca gctactctcc gttcctgatt gcgaaaaacc
    15601 aacgactagt gggtcaggtg cttcctttgt attaggtgta caccccatca tttggtaagc
    15661 gattacaaca agggctaaaa aaaataaaac acgtttcact ttaaatcact ccatctggat
    15721 ttgttagtac tatcttatca gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa
    15781 aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt acacctaaaa aatttaaagt
    15841 aaatcccata aaaaaatcaa tgttaacgct ttctttatta gctatactcg gtgtatcaat
    15901 tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt
    15961 ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg
    16021 gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac
    16081 aacatggtct cggaattaga aaaactgggt tataaaacag atcttcaata tggagaagat
    16141 aaagtagaaa atcaagtagc acaaatagaa aatatgatta caaaaggggt agatacactt
    16201 gtgattgctt ctatcgatgg ttcggcttta actgatgtgt tagctaaagc aaaagaagca
    16261 gatatcaagg tgatcgccta tgatcgttta ctgatgaatt cagaaaatgt cgattattac
    16321 gcgacttttg ataattttgg agtaggtgta tcgcaagcag cttatattga agagcactta
    16381 ggattgaaag aaggaaaagg accattcacg attgaattat ttggtggctc gcctgacgac
    16441 aacaacgcac taatcaatta caacggagtg atgtctgttt tacaaccata tatggacaac
    16501 ggtcagttgg tcgtgccatc tggacagacg agtttcagtc aaatcgctac gcttcgctgg
    16561 gatggttcta cggcgcaagc aaggatggat aatctgttaa gcgcaaatta tactgaccaa
    16621 acattggatg ctgttttgtc gccatatgat ccaataagtt taggaatcat ttcgtcatta
    16681 aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg tgattactgg acaagatgcg
    16741 acagttgctg gcgttaaatc gatcattgcc ggggaacaaa cacaaacgat ctttaaagac
    16801 acacgtatct tagcaaaaaa tacgattgaa atgatcaaag caatcagtga tggagaagag
    16861 gttccggtca atgatactga aacttatgat aatggggtga aaactgtccc tacgtattta
    16921 gcaaatacag tatctgtcga taaagataat tatcaagcag agctgatcga caccgattat
    16981 tataaagaat ccgatctgaa aaattaagag gaaccaaaca aggaactaaa gagatctaag
    17041 tcagagagag gataagaatt atggcggatt gtattttaaa aatggcagat atcaccaaaa
    17101 aattttctgg aatccgagcg ttagattgcg tgaatctgat gattgaacgt ggggaaatcc
    17161 atgcattgtg cggagaaaac ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct
    17221 tgtatccata tggctcctat gacggggaaa tcttttatga tggaaagctg tgtcaattta
    17281 aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa
    17341 gtccttatct ttcaataaaa gagaatattt ttctcggaaa tgaacaagca gaaagaggcg
    17401 tgattgattg ggaactgaca gaaaagaaaa catatgaact gctgaataaa gtcggtttgt
    17461 ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa
    17521 tagctaaagc attatcaaaa tccgtccgtc tgttgatttt ggatgagcct accgctgcat
    17581 tgaatgaaga agaaagcgcg aatttgctga gattgatccg agagttgaaa gagcaaggtg
    17641 taacctccat cattatttca cataagctga atgaaatcgt ggatatcgca gatcgaatca
    17701 ccattttacg ggatggacag acgattgaga cactagaaaa tgaaatgatc agcgaagagc
    17761 atattattcg cggaatggtg ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg
    17821 atattggaga agtttatttt aaagtagaag actggacggt ctatcaccct cttgatcacg
    17881 agcgaatcat aaacgaccac atcaacttgc atttgaaaaa aggagagatt gtaggaatag
    17941 ctggactgat gggagcgggc agaactgaat tcgctatgag tctatttgga cattcttatg
    18001 gaagcaagat atccggacgt atattaaagg aaggggcaga gatatcagtt aagaccgttc
    18061 cttcagccat tgcaaacgga ctcgcttatg tctcggagga ccgaaaatct ttaggactga
    18121 atctgctgat ggatattcgg gaaaacgtga cgctgagcag tttggataaa ataagtcaaa
    18181 aaggagtatt agataaagaa aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc
    18241 gtataaaaac aaactccatc catcaaattg tcagtagtct tagtggtggg aatcaacaaa
    18301 aagtcgtttt atccaagtgg ctgatgacac aacccgatat tctttttttg gatgagccaa
    18361 cccgtgggat agatgttggg gcaaaatatg agatctacac gattatcgaa gaaatggcag
    18421 caaatggcaa agctgtctgt cttatctctt ctgaacttcc tgaaatatta gggatgtgtg
    18481 atcgaatcta tacgatgaac gaaggacgta ttacaggaga agtctcgcga gaagatgcag
    18541 atcaggaagt cttgatgaaa ttaatgacga aagaagaggt gggttaagtt ggaacaggca
    18601 aaaaatctta cggataaaaa agagaaaaaa ctaaatttga aaagtagagc agctgatctt
    18661 tttggtcgct acagcatggt gatcatttta gcgggactac ttattgcgtt tcaattgatg
    18721 acggatggta ttttctggcg tccattgaat ataactaacc ttgttctgca aaatagccat
    18781 atattagtac tagctgcagg gatgttatta gttgtgctct taggtcatgt ggatttgtcg
    18841 gtagggtcag tgatggcgtt tgttggtgca attgcgggca tgatgatggt caatcgccaa
    18901 atgaacccgt ggatgacagt tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg
    18961 caaggttttt gggtagctta tgcagggata ccggcgttta tcgtgacttt agcaggattg
    19021 ctgatgtttc gtggactgac acaagttgtt ctaggaggcc aatctctagc accttttcca
    19081 gctgttttcc aaaaaatgtc tacgggttat ttaccagact taagcgaagg aagcagcctt
    19141 cacttcttga cattgatttt aggaatgatc atcacgatcg tgttagttgt tggtcaatgg
    19201 cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg aatctccaac agctttcttg
    19261 gtaaaggcgg tatttacagc agtcgttgta ctaggaataa cttatatctt tgcctcttat
    19321 caagggtatc cgattatttt gatcattctt ggtttgatcg ttgcagcata tgggtttttg
    19381 acaaataaaa cagtagcagg tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct
    19441 gaactatcag ggatcaaaac aaagcgaatc acgttctggg tattcgtcaa catgggagtc
    19501 atggcagctt tagctggatt agttttagct gctcgattga atgcggcaac acctcaagca
    19561 ggtacatcga tggaactgga tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc
    19621 ggcggcattg gaacaatcat gggtgcgatt gtcggtggct tggtcatggg tgtattgaac
    19681 aatggcatgt cgattttagg cgtcggggta gactggcagc aggcaatcaa aggattgatc
    19741 ttgcttttag ccgttgtgct agacttgtat aataagaaaa aacgaacaac gtaggagaac
    19801 gccggttatt cagaaaaaag actgtgacaa gacatttgtc acagtctttt tttcgctaaa
    19861 agcgaacatt cattgtttcg tttgtataga ctaaaaataa tatatgtttt tttaccattc
    19921 gtataaattc tctttttgct cttgattttt ttgcttaatt gagttaaatt acatagggcg
    19981 ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt gctggatgtg aacatcagga
    20041 ggataatgta atgaaagtat ttgattacga agatattcaa ttaattccta ataaatgtat
    20101 cgtaaatagc cgttctgaat gtgatacaac ggtcacatta ggtaaacata cattcaaaat
    20161 gccagtagtg cctgccaaca tgcagacgat tattgatgaa acaatcgcag aattcttagc
    20221 ggaaaatggt tatttctata tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat
    20281 caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt gttggcgtca agaaagagga
    20341 atattccttt atagagaagt tggcagaaga atcgctaaat cctgattata tcactatcga
    20401 catcgcgcat ggacatgcta actcagttat tgatatgatt cagcatatta aaaaatattt
    20461 gcctgaaact tttgtgattg ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga
    20521 aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca ggaaaagtat gtattacaaa
    20581 aatcaagaca ggctttggga ctggaggctg gcaactggca gctcttagat ggtgtgcgaa
    20641 agcagctcgc aagccgatca tcgctgacgg tgggatcaga acacatggcg atattgcaaa
    20701 atctgtccgt tttggtgcga caatggtcat gattggttca ttgtttgccg gtcacgagga
    20761 atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc
    20821 ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca
    20881 caaaggatct ttgaaagata cactagtgga aatgcaacaa gaccttcaat catctatttc
    20941 ttatgctgga ggaagagatc tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa
    21001 ttcgattttt aatggagaca caatctaaaa aataaataaa atattaggat gttgtgaaaa
    21061 agttgtcctg cattaagtaa taagaacagc caaacaaaaa tagttcttta tatgttttgt
    21121 ttggctggaa ttattatcgc agatacaagc ttttgaacat cattattcag aataagggac
    21181 tgtgataaga tttttgtcac agtcccttat gttttgcaaa aaaagttttt cttttttctc
    21241 gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa aagagaagct tatgtgggaa
    21301 aaagtagaga aaaaacaagg accgatttat cgtcaaatta tggaacagat tatgagagaa
    21361 atcgaaaacg gaaggatcaa tcctggaagt caacttgaat cagaacgaaa actagggcaa
    21421 ctttttggtg tgaatcgttc caccgttgtc catgcattgg aagagcttag ggagcttggg
    21481 gtactaacga gcaaacgtgg cagcggatgg tttgttaatc agactgaatg ggggaaattc
    21541 gctgttccaa gaatcgattg gcggcaaatg atctctcctc gttatgagca gacggactgg
    21601 tatgagcaaa aaataagaga agcaaagcag atccaaaaaa catcgtttct tgatttatac
    21661 gctagcgaga tgccagatga gcttttgccg aattttgagt ttccttctta ttctcttgaa
    21721 gaaatcttag cggaagaaaa agaaatggat cttttaggct atactccgtt acgtaacaaa
    21781 atcaaacggt atttggaaag taaatttgaa ttggcatttt cccccaacca gcttcttgtg
    21841 actggtggcg gtcagcaagc aatctttttg attttacaaa cgattctttc agctggagag
    21901 gggatagctg tagagtcacc ttcttttttt taccgattac ctcttttcaa agcttcagga
    21961 acgcgtttat ttgggatacc gatggatgaa gaagggatcg acttagaaaa actgaaagaa
    22021 tcgatccata aaaataaatt gaaagcagtg cttatcaacc cgaattttca aaaccctaca
    22081 ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag aattgtgtcg acagtatcgg
    22141 cttccaatta ttgaagacga tgtatttacg gatctttcat ttctaaacca agaagaagtc
    22201 cttcctatac gaacgattga tccggataat gtgttatacg tcggttcgtt atctagggtt
    22261 ttaggaaaaa caacgaaaat cggttgggtg ataggacctg agacttttat ctcgcaacta
    22321 gctcaagcgc aagaaatgat ggagttttcg atgaatatcc ttacacaaat cacagtggct
    22381 aatgtattta atgaagcaat tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa
    22441 aaaagccgac tgataagcgc ttggggagat tcacaatcgt ttttcaagct tcattcaatt
    22501 ttaggtggtt actatgcctg gattacttgg gatgggaaaa aactagaccc cgaactagca
    22561 gaaaaagtag tagatatggg tcttggaatt gcaccaagtc tactttttgg gcaagatata
    22621 aatggaatca gaatcaatta cagtaggctg gatgaaaacc aaataccgtt gttttattca
    22681 aagatgaaaa ttctagaaaa gtggttaaca gaataatttc acaaagtaaa tagattaaag
    22741 aatataacag cttggattta aatttcaagc tgtttctttt ttttcggaaa aagaatacga
    22801 tataaagaaa ctaagaaaaa accattattt gtacgtccgt tcacgatgtg taaataaaat
    22861 aaatgtttgc aacaaaactg tataacacca acgtttttaa atagaaacag atggttgtaa
    22921 gtttgattag aaaatagaaa aaatgcagat gaaatataaa acgctttcct tattgtgatt
    22981 ttattcacta atgctgttaa tatgcggatt ggcataattt cctttcatcg tgaaaatttg
    23041 tgcttttagc ttgtatttct cataaatcca tgataattta ttaatgaaga cggttaaata
    23101 gtgatacagt tttattcact gtttcaatcg cagaatgaag agattttctt atagatgaga
    23161 gaggaatgtg tgacaaatgg caaacaaaaa agcaccgatc gacttccaag cactgctaga
    23221 tgaagtcaat gcagatttcc cagtatatca agcacttgat caagatggaa acgtagttaa
    23281 caaagattta gtacctgatt tatccgatga tgaactcgtt gaattaatga ctcatatggt
    23341 atggtctcgt gtattagacc aacgttcaac agcgctgaac cgccaaggac gcctaggctt
    23401 ttttgcacca actgctggac aagaggcaag tcagctggct agtgcttacg catttgataa
    23461 agaagatgtt cttcttccag gttatcggga tgtacctcaa ctagtaaaac atggtttacc
    23521 tttatctcaa gcttttttat ggtctcgagg acatgctgca ggtaattctt atccggaaga
    23581 attgaaagct ctaccaccac aaattatcat cggtgcacaa tatgtacaag cagccggtgt
    23641 tgcattaggt ttgaagaaac gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg
    23701 cggttcatca caaggtgatt tttacgaagc aatcaatttc gctggcgctt accatgcaaa
    23761 tgccgtattt tatatccaaa acaacgggta tgcgatctct acacctcgtg aaaaacaaac
    23821 tgcagcaaaa acacttgcac aaaaagctgt cgcagctggg attcctggta ttcaagtcga
    23881 tggaatggat ccgcttgcag tatatactgt atctaaaatg gcacgggaat gggcgatcag
    23941 tggaaatgga ccagtcctaa tcgaaacact gacttatcgt tatggaccac atacactatc
    24001 tggtgacgat ccaacccgct accgtacaaa agacacagat gacgattggc agaaaaaaga
    24061 tccattgatc cgcttccgta aatatttgac ggaaaaagga ctttggtctg aagaaaaaga
    24121 agaacaagtc atcgaagcaa ctaaagaaga aatcaaagca gctattgctg aagccgacaa
    24181 agtaccaaaa caaaaggttt cagatttctt gaaaaacatg ttcgaggtat ctccacaatc
    24241 aatcaaagaa caaattgcaa tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa
    24301 acaatgatcc aagcaatcac agatgcctta gcacttgaac tagaaaatga cgagaatgtc
    24361 gttgtttttg gtgaagatgt tgggaaaaac ggaggagttt tccgggcaac tgaaggattg
    24421 caggaaaagt tcggagaaga ccgagtattc gatactcctt tagctgaatc tggaatcgca
    24481 ggattgagtt tcggtcttgc gttagaagga tttcgtccag ttcctgaaat ccaattcttt
    24541 ggttttattt tcgaagcaat ggacgaagta gtagctcaaa tggctcgtac aagatatcga
    24601 atgagcggca ctagaaatct acccatcaca attcgttcgc catttggtgg cggtgttcat
    24661 acaccagaat tgcattcgga taatttagaa ggtttgatcg ctcagtctcc tggtattcga
    24721 gtagtgattc cttccaaccc ttatgatgca aaaggactat tgatttcttc tattagaagt
    24781 aacgatccgg ttgttttcct agaacacatg aaactatatc gctcattccg agaagaagtg
    24841 ccggatgaag cttacgaagt tcctttggat aaagcagcag ttactcgtga gggaacagat
    24901 gtatctatta tcacatacgg agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt
    24961 gcaaaagaaa atatatctgt tgaaatcatt gatcttcgta cagttgcgcc attagatgta
    25021 gaaacgatca ttcaatccgt ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa
    25081 cgacaagcgg gcgttgccgc gcaagtagtt tctgagatct cagaacgcgc tatcctttca
    25141 ttagaagccc caatcggacg agtttctgca cctgatacag tcttcccatt tggacaagca
    25201 gaaaatgtct ggttgccaaa tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa
    25261 ttttaagaaa agaagggacg aaacaaagtc atggcttatc aatttaaatt gccggatatc
    25321 ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc
    25381 aatgaagatg atacattact agaagttcaa aatgacaaat cagtagaaga aatcccgtca
    25441 cctgttacag gaacggttaa aaatgtgatt gttccagaag gtaccgtagc aaatgttggg
    25501 gatgtattag tagaaatcga tgcaccagga cacgaagaca atgaagggga cagtggtgta
    25561 gccgcagaat cccaaactcc agctaaaccg gctgctgaac ccactgttga tacagaatca
    25621 gctggatcgt caagcgaagg agtcttccaa tttaagttac cggatatcgg tgaaggaatc
    25681 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25741 acattactag aagttcaaaa tgacaaatca gtagaagaaa ttccgtcacc tgtcacaggg
    25801 acagttaaaa acgtgattgt tccagaaggc actgtagcaa atgttgggga tgtattagta
    25861 gaaatcgatg ctcctggaca caatagtgca ccgtcaacta gtgcgccatc agctgaagca
    25921 ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg ttgaagctgc cgatccaaat
    25981 aaacgtgtat tggctatgcc atctgttcgt caatttgccc gtgaaaaaga tgtagatatc
    26041 tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa aagaggacat cgaaaacttc
    26101 ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag cgccagaagc ggcagcaccg
    26161 aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac cagcaaaacc attcaagtcc
    26221 aacttaggag acttggaaga acgtgtagca atgacgccaa cacgtaaagc tatcgctaaa
    26281 gcaatggtca acagtaagca tacagctcca cacgtgacat tgcatgatga agtagaagtg
    26341 tctaaactat gggataatcg gaaacgattt aaagaagttg ctgctgcaaa tggtacgaaa
    26401 cttactttct tgccatatgt cgtgaaagct ttaacagcga ctgttaagaa atacccagta
    26461 ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt ataaacatta ctacaatatc
    26521 ggtattgcaa ctgatacgga tcacggacta tatgtaccaa atgtgaaaga tgcagaccgc
    26581 aaaggaatgt ttgctattgc agacgaaatc aatgagaaag caaaattagc tcatgacggc
    26641 aaactatctg cagaagatat gcgcaatgga acaattacga tcagcaatat cggatctgta
    26701 ggtggcggtt ggtttacacc agtcatcaac tatccagaag tagctatttt aggtgtagga
    26761 acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa tcgttgtcgg tcgtgtgatg
    26821 aaactgtcat taagtttcga tcaccgtatc gtagatggag caactgcgca acaagcaatg
    26881 aataatatca aacgtctttt agctgatcca gagctattaa tgatggaagg atgataaaca
    26941 aatggtagtt ggagattttg ctgtagaatt agatacagtt gtaattggtg cgggacctgg
    27001 tggctatgta gctgccatcc gcgctgctga aatgggacaa aaagtagcaa tcattgaaag
    27061 agagtatatt ggcggtgttt gtttgaatgt cggctgtatt ccttcaaaag cactgatagc
    27121 tgcaggacac cattatcaag agtcactaga ttctgaaatg tttggagtaa ctagtgaaaa
    27181 cgtcaaacta gattttgcaa aaacacaaga atggaaagaa aacaaagttg tccatacgtt
    27241 gacttctggt gttggtttcc tattaaaaaa acataaggtt gaaacaatcg aaggagaagc
    27301 gttcttcgta gatgatcaca cactacgcgt cattcatcca gattcagctc aaacttattc
    27361 gttcaataac gcaatcatcg caacaggttc ccgtccaatc gagattccag gatttaaatt
    27421 cggaggacgt gtgttagatt ctactggcgg attagcattg aaagaagtac ctaagaaatt
    27481 tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc ggagcgtacg caaacttagg
    27541 agcagaagta acgattcttg aaggctcacc acaaatttta ccaacttacg aaaaagatct
    27601 agtaaaatta gtagaagatg atttcaagaa aaaaggcgta acagtcgtaa cgaatgcaat
    27661 ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg aaatatgcag tggacgggaa
    27721 agaagaatcc gttactgctg attatgtgat ggtcactgta ggtcgtcgtc cgaatacaga
    27781 tgatatgggc ttagaacaag cgggtgtcga agtaggagaa cgaggattga tcacggtgga
    27841 taaacaagga cgtacgaacg tacctaacat ctatgccatc ggtgatatcg ttccaggggc
    27901 ggcattagca cacaaagcca gttatgaagc aaaaatcgct gctgaagcaa tctctggcaa
    27961 aaaagtagct gtagattata aagcaatgcc ggcagtagcc ttcactgatc ctgaattggc
    28021 ttctgtaggt atgacgatca aagaagcaaa agatgctggt atcgaagcaa aagcatataa
    28081 attcccattt tcagggaacg gtcgggcatt atcgcttggt aaaacagaag gattcatccg
    28141 tctagttacg acgatcgaag ataacgtgct tattggcgcg caaatcggtg gagtcggcgc
    28201 aagtgatatg gtctctgaat tagctttagc aatcgaatca ggaatgaatg ctgaagatat
    28261 cgccttgacg attcatcctc atccatctct tggcgaaatc gtcatggatg cttcggaatt
    28321 agcattaggt ttaccaatcc atatttgatt tgaataatac aggcgaaacg caactgaaaa
    28381 atcagttgcg ttttgttttt tagaattatc aataatatta ttatgtaaac ttaaaaatat
    28441 gacaacttct tggtttatta ccacttccta tgctacactg aagaggaata tgaaatagga
    28501 gagttgaaga tgcaagaata tcagtatcca cttgatttag attggacaac agacgaaatg
    28561 gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga atgaaaaagg gataaacaat
    28621 gaagcttttt tgaagactta tcagcagttt aaaactgttg tcaaatccat tggtgaggaa
    28681 aaacgattgg gacgggaatt tgaaaaagct tccggttatt ctttatatcg tacagtgcag
    28741 caagcaaaaa aaaatccaaa caaaactttg agaatgaacg ggtgagataa atggaacaga
    28801 tgattgatca tatcaagtcc tggcttgaag aagctggtga gaaaatcaaa tcttctcaac
    28861 aaacattaac aatctcgcaa aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa
    28921 acatccagaa ttttctaatc gaaaaaatcc attcctttga tccagaggcg aaaatattgg
    28981 cagaagaaga agggtgtaat caattatcgg atctcgatgg aagagtcttt atcatcgatc
    29041 ctattgatgg caccttgaat tttgtgattg aaggggaaaa tttttgtatt atgctcgctg
    29101 tttatgaatc cggcgttgga aagcttggtt tcatttatga cgtgatgaga aatgaacttt
    29161 tttggggtgg aaaaggaata ggcgtgtata aaaatgatga gccattgcct agaccagcaa
    29221 ataaatcctt atcggaaggc ttacttggtg tgaatagtta tcttttcggt cataataaat
    29281 atggaatccg agaaattgcg gaaagcagca tggggacacg tatgtacgga tgcgctggtc
    29341 ttgaactagt ggcaatcttg aaaggaaacc acattgctta tatttcaaac cttagccctt
    29401 gggattatgc agcgggaaat gtcttattgg aagagtttgg tatggtaaca agcggtcttt
    29461 ttggtactcc tttgacattt gaaaaaaggg aatactattt agcagggact tcttgtgcat
    29521 atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac
    29581 aaaaatgata aaaacgagca aaagattaat tttctcgaaa aaatgaaaac attttactat
    29641 atttcatgaa aaaagtctgc tataattagc agtcgagtaa aatttgccct gtcataaaga
    29701 accaataaaa aaggagttag ttacattgaa ttatagaaac gatatccgta acgtcgcaat
    29761 tattgcccac gttgaccacg gaaaaacaac attagttgat gaactattaa aacaatcaga
    29821 tacattagac gcacatacgc aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa
    29881 agaacgtggt atcacgattt tagccaaaaa cacagctgta gactataaag gtattcgcgt
    29941 aaacatcatg gatactccag gacacgcgga cttcggtggc gaagtagaac ggatcatgaa
    30001 aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg
    30061 ttttgtattg aaaaaagcac tggaacaaca tatcacacca atcgttgtcg taaacaagat
    30121 tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa gttcttgaac tattcatcga
    30181 attaggagca gatgatgatc agttagactt tcctgtgatt tatgcatctg cactaaatgg
    30241 tacgtcaagc ttgtcagatg atccagcaga tcaagaacca accatggcac ctatttttga
    30301 tacaattatt gaaaaaatcc ctgctcctgt cgataacagc gacgaaccat tacaattcca
    30361 agtttcatta cttgactaca acgattatgt aggtcgtatc ggaatcggac gtgtatttag
    30421 aggaacaatc aaagtaggag accaagtagc attaattaaa ttagacggaa cagttaagaa
    30481 attccgtgta acgaaattat ttggtttctt tggactaaaa cgtctagaga ttcaagaagc
    30541 aaaagctgga gacttgattg ctgtctcagg tatggaagat atctttgtcg gtgaaacagt
    30601 tactcctgtg gatcatcaag atgcgttacc tatccttcac attgacgaac caacattgca
    30661 aatgactttc ttagttaata attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc
    30721 tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca gatgtatcct tacgagttga
    30781 accaacaaac tctccagatg catggacagt ttctggtcgt ggagaattgc atttgtctat
    30841 cttgattgaa aacatgcgcc gtgagggata cgaattgcaa gtatctcgtc cagaagttat
    30901 tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc
    30961 agaagaatat atgggtagtg ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga
    31021 tatggtccat acaggaaacg gacaaatccg tttgacattc ttaacacctg cccgcggttt
    31081 gattggttat tcaactgaat tcctttcaat gacacgtggt tacggaatca tgaaccatac
    31141 ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct
    31201 tgtttcgatc gatacaggaa aagcaacgac ttattctatc atgagtatcg aagaacgcgg
    31261 tacagtcttc gtagaaccag gtacagaagt atatgaagga atgattatcg gggaaaacag
    31321 ccgtgataat gatttgactg tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc
    31381 tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc
    31441 tttggaattc ttgaatgatg atgaatattg cgaagtaaca ccagaaagca ttcgcttgag
    31501 aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct aaaaagaaaa aataatctgt
    31561 aaaaggatga agagagaaac cagcttgtta taaaaaatag ctggtttctt tttttgtcca
    31621 actcttttct gtggagaagt catctttgtc aatggacata aattcagaaa atacgccgaa
    31681 agacgtttaa aagactttta aaaaacttat ttctagagag tttttgtttc ataataagat
    31741 aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga
    31801 gaaaggagcg ggaagatgaa cgacttatta aaacaatact ttggctatga tgagtttaga
    31861 cctggtcaaa aagaaattat ccagaaagtc attgatcagg aaaatgtatt aggaatcatg
    31921 cctaccggaa gtgggaaatc aatctgttac cagcttccag ctttactatt agatgggctt
    31981 actgtggttg tgtccccttt aatctctttg atgaaggatc aggtggacgc ggcgaatcaa
    32041 ttaggtatac ccgccacttt tatcaatagc tcgttagatg gttacgaaac agctagacgc
    32101 ttccaagaaa tcgatagaca gcagtaccgt ttactttata tcgctcctga acgttttatc
    32161 atgccggatt ttatccaagc aatgaagcga tggaacgtcc gtatgatcgc tattgacgag
    32221 gcacattgta tctctcagtg ggggcatgat ttccgcccaa gttatttaca gatggcgaac
    32281 cagctagatc aactgccaaa tcgtcctgta attgttgctt tgacagctac agcgactgtg
    32341 caagtagctg ctgacataaa aagactttta aaaattccag aaaacaacca tattcagact
    32401 ggttttgaac gagaaaatct acgatttcaa gtaattaaag atcaaaaaaa agaacaatat
    32461 ctaatcgaat atttaaagat caacaaaaat caatctggta tcatttatgc agcaacgaga
    32521 aaagaagtag accgtctcta tcatttgctt aaaaaattcg atttttccgt gggtagatat
    32581 catggtggtc taaatgagaa tgagcgaaca gaaatgcaag aagccttttt atacgatcgc
    32641 ttgcagctga ttgtagcgac aaatgcattt ggtatgggta taaataaaag caatgtccgt
    32701 tttgttatcc attatcaaat tcctggctct ctagaagcat actatcagga agctggtcgg
    32761 gcgggaagag atggtctttc cagtgaagcc atcctgcttt tctctcctca agatatccaa
    32821 gtccaaaagt tttttatcca gcagtcccaa agagaagaag ggcaaaagca aaaagaatat
    32881 gaaaaattaa aagcgatgac agaatacgtg tatattgagt cctgtcttca gcaatacatc
    32941 ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt gtggtaactg tttagatgac
    33001 agagaaatag tagaagtaac gacagaagca cagatggttc tttcttgctt aaaacgtatg
    33061 ggtgagaact atggtaaaca aatgctgatg aaagtcttag caggatcgaa agaacaaaag
    33121 ctgcaagctt taggttttgg acatttatct acctatggac tgatgaaaaa ccaatcgcaa
    33181 aaagaaacga tgcagttaat tgaatatttg atatcgaatg gctatcttct gaccatcaat
    33241 ggtgagtatc ctgttttgaa agtaacagaa agagggatac aagtattgaa aggacaagaa
    33301 tctgtttatc gaaaggaacc caagaaagtc cagcagctat cggatgaaga aaccgataca
    33361 ctatttgaag tgctgcggga actgcgaacg gatctagcat cagaagcagg cgttcccccg
    33421 tatgtcgtat tttcagattc cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga
    33481 ttggagatgc ttcagataaa aggagttggc caaagcaagc ttgataaata tggcgaagcc
    33541 tttttatctc ggataaaaaa tgcggactct aacgttctgg ataagtagaa agaacgaata
    33601 cgtaaaaatg aataaaaaca actggttaaa tctgtttttt ttgctcctta tgaaaatcaa
    33661 atatgttata attaaggaat tagaatggga ggtaagcaca gatggattct ttgtcgaaaa
    33721 aacttgcgat ccaagtatta aatgatgaag cagaacggat caaacgatta atccgaaatc
    33781 aaaacaacag tctctgtatc tcacaatgca aagccttcga agaagtggta gatacacaga
    33841 tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg
    33901 agggccagct gatgttaagt gagctagaac gagaattgaa tcagttatat agtgaggttt
    33961 accaagaaat ttatgataaa aaagaagttg gcaaggagga atagacttgc ctaagaaagt
    34021 gaaaaaaagg catctattag actatagcat cttgattccc tatttaattt tatgtgtatt
    34081 gggattgatc atggtatata gttctacgtc gtatctatta ttagaaaacg gacaaaatcc
    34141 gtccgcatca gtcattaacc agagtatttt ttgggtgctt agcttgattg ctatcgcact
    34201 actttataaa atgaaaacag atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc
    34261 agttttgacg attttgctgc tgattgttgt gttttttggt aaggagatca atggggcgaa
    34321 aggttggctg caaattgcag gattctcgat ccaacctgcc gaatacctaa aaatcatttc
    34381 tatctggtac ctgtctctga ctttatctaa aagacaaaac agtgtacaaa aagattttct
    34441 tgggactgtg aaacgtccac ttgcaatggt tatcggactt actgcattag tcgctattct
    34501 tcccgatttt ggtaatgcgg cagttatctt tttgattatt ttggttctgc ttttagccag
    34561 cggagtcaat tatgtgtata ctttgattgt aggagtagga ggcttttgtc taagtacttt
    34621 tacgatctgg ctgatcaata tcacaaacgg aaagatctta cccggacgtt tacagtatat
    34681 ctataatcga tttgctattt accaaaatcc tttctcagac gaattaaata aagggcatca
    34741 actagtcaac ggctactatg ccatgttcaa cggaggacta tttggcaggg gattaggtaa
    34801 cagtatccag aaaaaaggat ttttacaaga agcacaaacc gattttattt atgcaatcgt
    34861 agtggaagaa ctgggagtga ttatggggat tttgatatta gcgttgctat tctttatgat
    34921 tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat
    34981 tgggatcggc gcaatgtttt tgatccaagt atttgtcaat cttggaggaa ttactggcgt
    35041 tattcctttg acagggatca cgttcccatt tttaagtcaa ggaggctcca gtttgctgat
    35101 gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt
    35161 aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca
    35221 ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga attaggaatt aaaactgttg
    35281 gaatctatgc agaagaagat gaatattccg tacaccgttt caaagcggat gaagcatatt
    35341 tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga tattgaaggg atcatttcta
    35401 tcgcgaaaga atgcggagct gacgctattc atccgggtta cggtcttttg tcggaaaact
    35461 tgaattttgc gcaacggtgc gaagaagaag gcatcatttt tgttggaccc aaactgcatc
    35521 atttagatat ttttggagat aaaatcaaag caaaagctgc agctatcgaa gcggggattg
    35581 cttcgattcc aggcacagat ggaccaatcg cttctataga cgatgccttg gagtttgcga
    35641 aacaatacgg ttatccgatt atgatcaagg cagctctagg cggcggcgga cgcggcatgc
    35701 gtgtagctca cgatgaaaag agtgcaagag agggctacga acgtgcgaaa agcgaagcaa
    35761 aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata catcgctaat cctaagcata
    35821 tcgaagtaca gattctcggg gatacgcatg gaaacgtcat ccatcttttt gaaagagatt
    35881 gttctgttca gcgccgtcac caaaaagtag tagaagtagc gccttgtgtc tctatgaatg
    35941 aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg
    36001 tcaacgcagg tacagtagaa tttcttgttg aaggagatga tttttacttt atcgaagtca
    36061 accctcgcgt ccaagtggaa catacgatca cagaaatgat tacagatatt gatatcgtaa
    36121 caacgcaact gttgattgct caaggtttgg atctgcataa agaaatcgga ttgcctcagc
    36181 aagaaggaat taaattaaat ggttcagcca ttcaatgccg aatcactaca gaagacccgt
    36241 tgaataactt tttaccagac acagggaaaa tcgatacgta tcgttcacct ggtggttttg
    36301 gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt agtgacaccg tattttgatt
    36361 cattgctagt aaaagtatgt acgcatggtg ctacctttga aacagccatt cagaaaatgg
    36421 aacgttgctt gaaagaattt agaatccgtg gagtgaaaac aaacattcca tttatgctca
    36481 atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa aacaaccttc attgatagta
    36541 cagcaacatt atttgaattt cctcgactac gagaccgcgg aaacaaaacg atgaaatata
    36601 tcggtgaaat tactgtgaat ggttttccag gtatcgaaag tggcgaaaaa ccattttatg
    36661 aagaaccacg tatgcctaaa gatctaatca caagaactga ttacgtaacg gctaagaatg
    36721 tattagatgc gaagggtgcc gatgctttgg tcgaatggat caaaggacag gaaaatttgt
    36781 tgttgacgga tacgacattt agagacgccc atcaaagtct gctagcaact cgtgttcgga
    36841 caaaggattt caagcagatt gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa
    36901 gtgagatgtg ggggggagca acatttgatg tggcttaccg ctttttgaat gaagatcctt
    36961 ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac gttgctgcaa atgcttttca
    37021 gaggttcaaa tgccgttggg tattctaatt atcctgacaa tgtattggtg gaatttgtca
    37081 aagaagcagc agcgcaagga atcgatgtat ttcgaatctt tgatagtttg aactggactc
    37141 cacaaatgga aaaaagtatc caagctgtac gggatactgg taaaatcgca gaggcagcta
    37201 tttgctatac tggcgatatc aacgatccaa gtcgggcaaa atacaatgtt cagtattata
    37261 aagacatggc aaaggaacta gaacaactgg gtgcgcatat cattgcaatc aaagatatgg
    37321 ctgggctgct taaaccgcag gcagcttatc gtttgattag tgaattgaaa gaaacgacag
    37381 atctgccaat ccatctgcat actcatgata ccagcgggaa tggaatcatc acttattctg
    37441 ctgcttcaaa agcaggagta gacatcgttg acgtggcaat gagtgcaatg agtgggaata
    37501 ctagtcagcc tagcatgagc agcttgtatt atgcactggt caatggtcca cggctgccag
    37561 aaatcacgat tgaaaatgca cagaaattaa accattattg ggaagatgtg cgcatgtatt
    37621 acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga agtatatatg catgaaatgc
    37681 caggaggaca gtattcgaat ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct
    37741 gggatgaaat caaacaaatg tatcatactg tcaacttgat gtttggcgat atcgttaaag
    37801 taactccatc atctaaagta gttggagata tggcactatt tatggtccaa aatgatctta
    37861 cagaagaaga tatttatgaa aaaggcgaaa cactaagttt ccctgaatct gttgtgactt
    37921 tcttccaagg agaactaggg caacccgttg gcggatttcc taaaaaattg caaaagatta
    37981 ttcttaaagg cagacctgca ttgaatgaac gtccaggtct tttggctgaa tcagtcgact
    38041 tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta tgaaccaaaa caagacgaag
    38101 tattaagcta tttgatgtat ccgcaagtct ttttggacta tcaaaaagca tataatcaat
    38161 ttggcgatgt tacattactg gacacaccaa catttttcca aggtatacgt ttgggtgaaa
    38221 caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag
    38281 aacccgatat cgaagggaat cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa
    38341 tcactatcaa agataattcg atcatcagta cggtccaaac ccgattgaaa gctgaaccga
    38401 caaaccgaga acaaattgga gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa
    38461 aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa
    38521 cggccatcga agcacgtttt gacggagaag tcgcacatgt atatgtgtct tcaggagaca
    38581 ctatttcgtc tggtgacctt ttgattgaag tgactgagaa ataataagcg aaaggtgggg
    38641 aaacatgaaa agactgggtt tgttttggct gattctgcta ttagttattg tggcattcta
    38701 catggaacca gtcttgtttc cgccaaaact tgaagtgaag gaaaactctg atcaacaaat
    38761 atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa gcaactggtt tttctactta
    38821 tatcggaaaa ccgatcgatc agctggaaaa ggattttggc caagcctatg atcgattaac
    38881 tagcggattt ggttttgaaa caagatatta tgaaaataaa gctagtcatc tcacatttga
    38941 agcgaacatc caagacagca gagtcacaac tgttaaagta ctgcaaacag ggcctgctga
    39001 tatcgcgccg ttttatttag ggatgacgat gcaagatctt acgaaaatca ctactatcta
    39061 tactaatttc acttttgagt accatgatac agaagttgga atcgaactaa tggaagagga
    39121 tatgaattat cgtccgctaa ttgcttttga taacgacact tttgctattt tattttttga
    39181 tcaaacaaca aacggcctat tttcagttgt gtatttagat aaagatagtc ttttgaaact
    39241 attgccatat caagtgtttg gtgaaggcct tccacattat caagcagata aatcggctga
    39301 ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg actttattaa atatgcttag
    39361 aagagaagac gaacttccca tatacaatca aacacttgcc tttcaaaacc acagtcagct
    39421 gctactacat gattttttga caaggcctga ggaaatattg tcagaggaac gtatggctga
    39481 ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa ttctcactga cgaatgatga
    39541 attggagaag ttaatcagta aacaaaaatt agaaaatact agtgggctat ttattcatcc
    39601 ggcgattgac gctacttttt cgtttttata ttggtacagc gatccttaca accatgaccg
    39661 tttcatggac ccgggaacgg attctgtagg cattgctttt tctaaagaaa atatgttagt
    39721 cttattgcaa gaagaaaacg gagaaagtag tgatagcagt gatcgttaac gaagagctgt
    39781 ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg
    39841 cttcttatct tgaacataaa cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag
    39901 aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc ggcatatggt gagtatgcac
    39961 ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa aagacagctt gatatggatg
    40021 agcatgttgc agctttccga tatagtgaaa cacagctgca aagtctgttg gatcaaattg
    40081 tccaagtact tgccgaagaa gtttctaatg aaatcaaagt agatgcaggt agcccgtttt
    40141 ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca tgcaattaga caatagtgaa
    40201 tttgaaataa caaaacgacg cgggctgatc gtatgggtct acagcttaaa acaattaaag
    40261 aatctgaaaa agttcggctt gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt
    40321 tacttaaatg aagagacatt ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt
    40381 cgaaacgtag aacgttctta ccgaccggat atcgaaatga attttgccga aaaaatagga
    40441 aacaaaacag tattaaaaga cgaaggtttc gaaattgaag agctaaatac agaaatccga
    40501 ttagctgaac atctttaaaa atctcttatg agcagttttt aaataaaaaa taacttggat
    40561 aacgatcgat ttttgatcat tgtccaagta tttttttggt atactagtac cagtaccatt
    40621 tctaaggagt gagtagatgc ggataatatc aggaaattat ggcgggcgcc gtttaaaaag
    40681 tttagcaggg gcgaacacca gaccaactac agataaagta aaagaatcca tattcaatat
    40741 gattggacca tattttgatg gagagacagt attggatctg tttgcaggaa gcggtggttt
    40801 agctatcgaa gcagtatcta gaggctgcag tcatgcggta tgcgtagaca aaaattacca
    40861 agcgctaaaa atcatcaaag aaaacatcga aattacgaaa gaaccagaga aatttaccac
    40921 aataaaactt gatgcggaca aagcaatcga acggctagca gcggagaaac aacaattcga
    40981 ctacttgttt cttgatccgc catatgcaaa acaaaaaata atcgatcaaa tccataaaat
    41041 ggaagaactg ggactttttt ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt
    41101 cgaattgcca gaaactgtgg ctggattcca tcaaataaga aaacagattt atggcataac
    41161 ggccgtaact atctatagaa aagagggaaa tcaatgaaac gtgcattatt cccaggaagt
    41221 tttgatcctt ttacaaaagg tcacttagat acggtggaac gtgcggctaa attattcgat
    41281 gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa
    41341 agaatgacat tgatcacaaa agctgtttct catcttccta atgtaaaagt catgcatcaa
    41401 gaaaatcaat tgacggtaga aacagcaaaa gagattggtg cagatgcgtt gatcagaggc
    41461 atacgaagta tcaaagactt tgaatatgaa cgagagattg ctcagatgaa tcatcatcta
    41521 tatgatgaat tggaaactgt cttcttatta gcaaaaccag aatatagtca cgtgagttcc
    41581 agtattttaa aagaagtact gcactttggc ggagatgtga gcagctactt accgccagtg
    41641 atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac
    41701 agtactgatt atcatatttg gaatgttgat cattggattt gtagtagttc ccttgccgta
    41761 ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa ctaattacgg tcaatgataa
    41821 ggaagataaa ggttctgggt ctttctctct tacctctgtt ggtatcaggc aagctacagg
    41881 tttcttagct ttaaaagcta aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt
    41941 gactggtggg gcgacaaatg aagaatataa tcaaatgcaa cagtattata tggattcttc
    42001 ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac aagccttata aaatggaatt
    42061 caaaggcgta tatgtcatga gtgtagagaa aacatcgaat ttctttggca agctttcagt
    42121 tggagataca gtaacaaaag tagacgggaa aagctttcaa agtacagaag catttatgga
    42181 ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc agttatttgc ataatggtga
    42241 agaaaaaaca gcttctggtg atctgatcga gttaccaaca gataaaaagg caggaatagg
    42301 tatcacgctt actgaccata ccgagatcga atcagataca gatgttcgta ttaattctgg
    42361 ctctatcggt gggccatcag cgggattgat gttcacttta gaaatctatg aacaagtcac
    42421 tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact ggaacgatca acagtgaagg
    42481 agaagttggc cgaatcggag gaatcgataa aaaagtggca agtgcggata aagctggcgt
    42541 agagattttc tttgcaccag atgatacgat ttcagacgat gttaagaaag aatatcctga
    42601 aataaaaacc aactacgaag aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa
    42661 aatcgtacca gtaaaaacag tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa
    42721 ctaaataatt aaagaggggt tgtgacacga gttctgtcac aacccctctt tt
//
LOCUS       DACCWV010000008        38725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCWV010000008 DACCWV010000000
VERSION     DACCWV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.008"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(241..1185)
                     /locus_tag="IVW84_000420"
     CDS             complement(241..1185)
                     /locus_tag="IVW84_000420"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7916647.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1481..2425)
                     /locus_tag="IVW84_000421"
     CDS             complement(1481..2425)
                     /locus_tag="IVW84_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7916648.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2646..3860
                     /locus_tag="IVW84_000422"
     CDS             2646..3860
                     /locus_tag="IVW84_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7916649.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3882..5897
                     /locus_tag="IVW84_000423"
     CDS             3882..5897
                     /locus_tag="IVW84_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7916650.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5980..7353)
                     /locus_tag="IVW84_000424"
     CDS             complement(5980..7353)
                     /locus_tag="IVW84_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7916651.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7379..7663)
                     /locus_tag="IVW84_000425"
     CDS             complement(7379..7663)
                     /locus_tag="IVW84_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7916652.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7665..9680)
                     /locus_tag="IVW84_000426"
     CDS             complement(7665..9680)
                     /locus_tag="IVW84_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7916653.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9859..10164)
                     /locus_tag="IVW84_000427"
     CDS             complement(9859..10164)
                     /locus_tag="IVW84_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7916654.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10253..11284)
                     /locus_tag="IVW84_000428"
     CDS             complement(10253..11284)
                     /locus_tag="IVW84_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7916655.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11491..12366
                     /locus_tag="IVW84_000429"
     CDS             11491..12366
                     /locus_tag="IVW84_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7916656.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12518..13360)
                     /locus_tag="IVW84_000430"
     CDS             complement(12518..13360)
                     /locus_tag="IVW84_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7916657.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13452..15329)
                     /locus_tag="IVW84_000431"
     CDS             complement(13452..15329)
                     /locus_tag="IVW84_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7916658.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15326..16747)
                     /locus_tag="IVW84_000432"
     CDS             complement(15326..16747)
                     /locus_tag="IVW84_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7916659.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16750..20139)
                     /locus_tag="IVW84_000433"
     CDS             complement(16750..20139)
                     /locus_tag="IVW84_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7916660.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20396..21787
                     /locus_tag="IVW84_000434"
     CDS             20396..21787
                     /locus_tag="IVW84_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7916661.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21860..22507)
                     /locus_tag="IVW84_000435"
     CDS             complement(21860..22507)
                     /locus_tag="IVW84_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7916662.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22515..23201)
                     /locus_tag="IVW84_000436"
     CDS             complement(22515..23201)
                     /locus_tag="IVW84_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916663.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23207..24280)
                     /locus_tag="IVW84_000437"
     CDS             complement(23207..24280)
                     /locus_tag="IVW84_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7916664.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24502..25176
                     /locus_tag="IVW84_000438"
     CDS             24502..25176
                     /locus_tag="IVW84_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7916665.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25181..26521
                     /locus_tag="IVW84_000439"
     CDS             25181..26521
                     /locus_tag="IVW84_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7916666.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26670..27656)
                     /locus_tag="IVW84_000440"
     CDS             complement(26670..27656)
                     /locus_tag="IVW84_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7916667.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28380..29411
                     /locus_tag="IVW84_000441"
     CDS             28380..29411
                     /locus_tag="IVW84_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7916668.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29520..31052
                     /locus_tag="IVW84_000442"
     CDS             29520..31052
                     /locus_tag="IVW84_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7916669.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31191..32003
                     /locus_tag="IVW84_000443"
     CDS             31191..32003
                     /locus_tag="IVW84_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7916670.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            31993..32478
                     /locus_tag="IVW84_000444"
     CDS             31993..32478
                     /locus_tag="IVW84_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7916671.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32501..33046
                     /locus_tag="IVW84_000445"
     CDS             32501..33046
                     /locus_tag="IVW84_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7916672.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33119..34762)
                     /locus_tag="IVW84_000446"
     CDS             complement(33119..34762)
                     /locus_tag="IVW84_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7916673.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34891..37119)
                     /locus_tag="IVW84_000447"
     CDS             complement(34891..37119)
                     /locus_tag="IVW84_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7916674.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37152..37631)
                     /locus_tag="IVW84_000448"
     CDS             complement(37152..37631)
                     /locus_tag="IVW84_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916675.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            complement(37896..>38725)
                     /locus_tag="IVW84_000449"
     CDS             complement(37896..>38725)
                     /locus_tag="IVW84_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7916676.1"
                     /translation="FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGW
                     TPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSI
                     HEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKK
                     LNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGL
                     LRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT        11280 a         7357 c         7059 g        13029 t
ORIGIN      
        1 tgaatggtca aatacaagaa aaggtatttg gtcagtctcc aaaagtcata ttcttcatcg
       61 atcattaaca gacaaagaac tggcccgcca aggatatgag gacatctcct tgaagtacca
      121 gttcgtacac tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg tggtgtgaga
      181 ggggcggaag gtcagttggc tttcccctct actcgatttt tagttcatat tcattgtttc
      241 ttataataca gtgttcaata cttctcttaa agcacttgtt gatttgctga acattgcttt
      301 ttcttcttcg ttgattgata attcaacaac ttcacgaaca ccattttcgt caacgattga
      361 tggcacacct gtaaagatat ctttttcgtc gtattcacca gttaaataag cagatacagg
      421 caatacagct tgttcgttat tcaagatagc ttttacgata cgtgcagtac tcataccaat
      481 accatagtaa gttgcttttt tacgatcaat gatctcataa gcagcattac ggactttatc
      541 agcaatcaca tctaattcat cttgggcaat acgatggtct ttttctacaa tttcaaacac
      601 tggtttacca ccgacagttg tatgtgacca tgcagcaact tctgaatcac catgttcacc
      661 taagatatag ccgtggacgc tgcgtgggtc aacttttaat ttcaatgcga tttctttacg
      721 gaaacgagtt gtatccaatg ttgttccagt accgataaca cgtgatgttg gcagaccaga
      781 ttcgttccaa gcgatatatg tcaaaatatc tactgggttt gaagcaacaa cgataatacc
      841 gtcgaatcct gatcccatga tttcttttac gatttggcgc atgattgacg catttgtttt
      901 aactaaatct aaacgagatt gaccaggttt ttggttgata ccagctgtga taacaacgat
      961 atcagcatct ttacattctt catagccacc tgaccataca gccacatttt catcgcccca
     1021 agccatacca tctaaaaggt ccaatgcttc cccttctgct ttttcttgat tcacatcgat
     1081 caatactaat tcattagaga ttccttggtt gatcattgca taagcgatac ttgttccaac
     1141 aaaacctgta ccgacgatta ctactttacg acttgttttt ttcatgaata aacagctcct
     1201 tttcataact atttgttact ttcatgaaaa gtatagtctt gtttattcat tttatcaaca
     1261 tgttaattat aatagttgtt attataattt ttgatggaaa cgaattcctg aatacgcata
     1321 acagataaat gacaaatgtt tacacctata tcccctgata atacaaccaa aagtttagaa
     1381 ctgaaaataa atccaaaaaa attcgctgca acgatataat aagacagcaa tcgcacctaa
     1441 aacaattttc cttcatatgc tttagtgaaa gattttcttc ttaatgttta tttctccatt
     1501 cattgggagt acatgttgaa tacttcttga ataatttgaa taaagaagtc gtttgtttat
     1561 atccgatacg attagcgatt tcttccaacg aaaaactcgt ttcttgaatc agttccaacg
     1621 caaagtcgaa ttttttcttt agaaccagtt cttgaaagga agcatcaaaa tacattttca
     1681 atttattact aagatagacc ggattgtagc caaaatgagc tgctgtttct tttaacgtac
     1741 aagaagtaaa atgcttatcg atataatcta atactctaag catctcattt tctgcgcatc
     1801 catctgctga tgaaaagctt tgttgttcta ataaatgagt caattcgatc agtaatagtg
     1861 atgtatagag atttaacgaa cggatcttat gactgtatgt atcaaaataa atcaacagca
     1921 tattttcaat caaatgcgtc aaacgacttg tttttccgcc gcgaaaatga atgaattgtg
     1981 tatgttcccc tttttctttt gatgcattta tcaaaaaaga agaaagtaga ttatttgtgc
     2041 ttgccaaatg attcaatatc tctgtagata aggtttcttc ttttaataag atgttgatta
     2101 aaatatcctc ttgccctgtg tattcgattc tttgagtagc ttctttatca atgatcacca
     2161 cgtcactttc ttccatctct atcctttctc cattaataat ttgtaaacat gtaccgctat
     2221 acatataatt gatttcaaca aaatcatgtg tatgctcggg aacttccgca tatcgacggt
     2281 gtttactgat atgaatattt ccctgttgaa atagatcccc tcggtggatt tttggcggtg
     2341 tctgaagatg gtaagctaag tttaaatcat tgatattcat tccttgttca cgctgcagag
     2401 tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt cagctagttc caattgactc
     2461 cctccttttt gtaagcggtt tctttcgctt attagttcac gctgtactgc tattatatgc
     2521 aaagctattt ttggtttcat aaagactttc tatggttggg acatgaactt cctgtgattc
     2581 cgctattgag agaataacca cttcatacta tatttttgat aagcgcttac aaaaatgaaa
     2641 ggaacatgct tatgaataaa aagatgatga aattttctct gctgtcagtc tctcttattt
     2701 tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat gtcagctact tatgccacgc
     2761 gttctttatc tgctgttgaa ttattaacga ctgctccctc ttttatggtc actatttttg
     2821 ttttgattag tagttttatt gctaggaaaa tcggacaaaa acaaactgtg atcatcggat
     2881 tagtgattgc atcgatttgt ggtatcctcc ccctcttctc acaaagttac agtattgtct
     2941 tagcttcaag agctggacta ggtattggct ttggattgat caattctttg gcagtcagca
     3001 tgattgctgc cttttatcac ggagatgaac gagcctctat gattggtttt caaagtgcat
     3061 ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg acagctagta cagttcggtt
     3121 ggcaatatac ctttttagtc tacgccatca gcttacctat tcttgtcttg tttgttttgt
     3181 ttgttcctac acctgaaaca actgaacttc aatcgcatgg cacagcccca acaagacaag
     3241 taatcaaaac gaatcctaaa atgattagtt atggtatttt tctcttgctg gtcatcgtgg
     3301 tatataatgc tgtatcgatt aaattagcca cagtcctgac gacttattct ctaggaaacg
     3361 aaactaatgc agcgaatatc ttaacaatca tgcaattggc aagtatggct gccggagttt
     3421 gttttggctg gcttcagaca aaagcacatc gctatatgct tacactttct ttactactga
     3481 tgggaatcgg ctttgtattg attgccactg tgataaacct gtatgtgatt gggtttggtg
     3541 cgatcctaac aggaatttct ttttcgttat tcattcctta cttattcaac caagtaagta
     3601 tccaagcgcc caagtacgcg gagagtttca atacttcaat cttattggtt ggcgctaatt
     3661 taggtacttt cattgcacca tatggtttgc tctttctctc ttttgcttct cttgggagta
     3721 aaacacttgc tgttttcgtc aatggcagta tcttgctgtt gattagcgcg cttattagcc
     3781 ttctagtagt acacaggacg aatagaaaga cagtgataag agtagatgaa gaaataaaag
     3841 aacaattgac aacaaaataa atttgatgga ggtcgtgtaa aatgacaaat gaaaaacata
     3901 aagatagatg gctttggtat cctggagact ttgagatccg ccatggactg ctacaaaatt
     3961 tccaacggga agaacgaggt tttgactggc cagcgtactg gtacatggat gattgccatc
     4021 ggaacgtcaa gtttaagaga tattactttc ttgatcaacc ttccatgttt aaagtgacga
     4081 tccaaggagt tgggtatgta gaaattaatg gacaaaaaca tccatgtgga aaatggctga
     4141 cttgtccagc tggtaaagca aaaattcgta tctttgtagg acatacatca gggctaccag
     4201 cgatgtttat cgagggagac gaagtaaaat cagatattgg ctggacagca agtaatttta
     4261 ttgaagaata tccagctggc tggagtccgc tttatgtcga tatcgcaaaa gatccaaatc
     4321 aaatctatta tcaaaaagaa tgcgtccatc cagtcactga aacggagatt ggcggaggaa
     4381 tcttattcga ttttgggcga gcagtgaatg gctgtgtaga actaatatct ctggatgacc
     4441 atatcgaatc tatcactcta tgttatggag agtccgatgt agaagctatg gatatcgatt
     4501 attgctacta taagcaaata ggaatgacaa gaaaaatgaa agctcgtaaa cgcgcttttc
     4561 gctatttgtt tataccagag gtttctactg gaatggttca acttcaagtt tttcatgaat
     4621 ttttatctct ttcgaaacaa gctgcttttt caagcgattc ttctttactg aatcagatct
     4681 ggtcagtatc agaagaagcc ttcaagttat gtagtgattt gttttttatc gacggtatca
     4741 aacgagatcg ctggatttgg tctggagatg cgtatcaatc attcatgatc aatcaatatc
     4801 ttttttttga cgaggaaatc aataaacgaa cgatattagc attaagagga caaaatgaaa
     4861 tcaagcagca tatgaatact atcgttgact attcgatctt gtggttgatt ggtatcgaaa
     4921 accactatat gatgacgaaa gactatgaat ttttacgcat cgtctaccct aagatgcaat
     4981 cattaatgag atacttgatg gagcaaacga atgaattagg gtttatttat gggagagcaa
     5041 aagattggat atttattgat tggtctgaaa tggataaaga aggaacgctt gctgcagaac
     5101 aagtcttatt actgaaagcc tatcattcca tgatcacttg cggagatatc ttagctgaaa
     5161 catatggaga gaacgaaaat tatcatactc atctctatca agaaaaatat caactattga
     5221 aacaaaacct atttgactac ttttgggatg aagaacttgg cgcttttatc gattgctatg
     5281 aatctgggag aagaaatgtg acgcgtcatg cgaatatttt cgctgtttta ttcgattttg
     5341 ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt attgaatgac actatcacac
     5401 agattactac tccatatttc aaattctatg aacaagatgc tttatgcaaa ctcggacaga
     5461 cgaatatcgt ctaccaaacg attcttgatt attggggagg gatgttatct catgatgcgg
     5521 taactttctg ggaagaatac gaccctactc aaacaggaaa tgaaaagtac agtatgtatg
     5581 gagatcctta tggaaaaagc ttatgccatg catggggagc aagcccaatc tatcttttag
     5641 gacgctattt tatcggtttg tatccaacaa agccaggtta tgaagaattt atgatacaac
     5701 ctaatctaga ttcatttgaa acactgaatt gtacgctacc tatcaaagat ggaaagattt
     5761 cgcttgaaaa aacaaaagat aagatcgtga ttacttctga tagaacaggc ggaacagtga
     5821 aaatgaatgg taaaagttat cctcttctag caaaacagcc acttgtctta acaagctatt
     5881 caaaaataaa tgcataaaac aaaaaactgc ttgcgacata tcagaagact ttaaatcttc
     5941 ttctgatatt gtccaagcaa tttttatttt gaagtttgct taattctgtt ctttagaaac
     6001 aatgtttgtt ctttttccgg caaaaagaaa gctagataat acgacaagta aaaagaaagc
     6061 gatgattcca actgtcaagg ctatttgacc gtattctcct agtttcccaa gtacaagtcc
     6121 tggtactaga taatcagtat cggcaaatgt agaagaagcg attttaagat ctcctaatac
     6181 tggaaggata aataatggca gccaactaat caataagcta ttgaaagcag aacctaatac
     6241 cgcccctctt ctaccgccag tcgagttgcc gaagacacca gaagcagctc cgcagaagaa
     6301 atgtcctact accccaggaa tgataactgt agtttgcaag cctatcatag caaacatgga
     6361 tagtgttcct acaaagaagc taacaaaaaa gccaattaat acagcattag gtgcatatgg
     6421 aaaaacgata ggaacatcta atgctggttt agaatcgggt acgagttttt tagcaatccc
     6481 ttggaatgct ggaacgattt ctgccaacac cattcgaacc ccttgaagga cgataacaaa
     6541 cccagctgtg aatgttccag cttggatcag tgcaaagatt acagcgttgt ctccattgct
     6601 tagttctctt tcaacaaatc caggaccagc taacaaagca agtgcgacat atacgatcga
     6661 catcaagagc atgatcgata cagtagaatc tcgcaaaaag ctgaaacttt taggaatctt
     6721 tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt ccgatccatc cactagaagc
     6781 ataaccgata tttccggtat gccctaacgc aacttgattg tttccagtga tgttcttcat
     6841 aaacggttga caaagagcag gcgtaatagt taatagtgtt ccttcgaaga ccccgcctaa
     6901 taagatcatc cacaaatttg cagtaaaccc cgcgcttact aaaatgactg ctgttagaca
     6961 agaaacataa agcattgctt gacctgtaag aaaaatgaat ttatatcttg tgaatctagc
     7021 aagtaaaata ttcacgatca tccctactag catgatcaaa gcagtaggtg taccgtattg
     7081 ttgcaaagca atcgccacta ctgcttcatt gctaggaact actccttgag tatgcagagc
     7141 tgtttggaac atcgaagcaa aaggggttag tgaattactg attaaaccag caccgccgat
     7201 cagtactagg aatccgacaa atgttttgat agttccttgg atcacttctg aaaatttctt
     7261 cttctgaaga acgagaccaa gcagtgcaat caaagaaacg aggatggagg gctgactgac
     7321 gattgacaca aacaaattca acaatttatc cattgatagt tcctcccttg aatcattttt
     7381 attcttttga caagatcttc gttacttttt cttttacttc ttcgctgtca atgatcgatt
     7441 gcaaaggtac gagcttttca tcaggtatag aagttagcgt atctttcaaa gaatgctcaa
     7501 tgaagaaata atcagccata tcgctagata cacttccaac atccgtatga cttacttcaa
     7561 actcgtccac atctgctcca agatctgcaa gtgcttgttt gatgttcatc tctaccataa
     7621 aacttgttcc taatccactt tgacatactg caaaaatttt catatcattc gtctcctttt
     7681 cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta ataaatcttg aagattttgt
     7741 ttattagaaa gtagctttgt taaatttgcc aaagcccgaa gatgtgcctc attatcgata
     7801 gcagctaaac agatgaacaa actaatggga tgggctggat tatcaagcag atccacaggt
     7861 tgttgaagtt tcagcaaaga catccctact tcgtttacgc catcttctgg acgagcatga
     7921 ggtaatgcga tgtgatcgcc aatatggata aacgcaccat actgctccac gcgctcgatc
     7981 atagctttga tataactttc ttctatttta ttttctgata atagcggctg tgcggcaaat
     8041 gtgattgctt cttgccagtt ttttggttga tccgataatt ggatcatttc ttctgttaat
     8101 aattcagata gcataggacg ttgatcctcc ttcttttcta aaagtttgtt cattttacga
     8161 tttaatactt gatagagctt ttcttcgtct acgccttctt ttaattcgat ataaggcagt
     8221 agcgccgaca acaattcttg ggcagaaggc attgaaaatc ctggtatcag ccaatctctt
     8281 tgtacttgat tgatcagctg atttttttcc aagtcagtca tgattggatg cagaacatag
     8341 agagactttt tcttatcagt aagtgtgaga ggtacagtag aaaaaataat atcgtaagaa
     8401 gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct ctgtaaaaaa gatcatcggg
     8461 aataattgct gaagttcact ttttaagatc aatgaagagc taatgccact tggacagacg
     8521 atcacggctc ttatttttct atttctttct tctgcgtccg cttttctgat ctctccgcca
     8581 aatagtatag tgaaaaaacc aatctcttct tcagggatgc tcttccctat tcgtctttct
     8641 aacggaagta atgcttttct tgtcatttcg tagatggaag catattgatg cttaatttga
     8701 tcgatcatca cattaggtaa ataaaaagaa taggaaatac gaaaataggc agggaccaag
     8761 tgataaaaga cattcatcaa taattcacga aacgaagcaa attctacggc catcaggaat
     8821 tccatgcgat ggatgacttc tgaagaacat tccagcaaaa aatcaagtgc aggctcctgg
     8881 acatctcctt caacgatcga tgctaagcaa agagagaaaa atataatctc cgaattttga
     8941 tgttcggaat agccaaggcc agtcaatact tgtcgactaa tgtcagtgac tgcaagagaa
     9001 gccaacagtt cagcatcttt taccagttcc atggatgttt ttccaattcg aatggttgtc
     9061 aaactcaaat aataagctaa ttcatccatt cgatttggtg cgaatcgaag atctgatagt
     9121 gaaatcgttt tatctagcaa atctctaact ttggcgtaat aatcaatgga aagggttgcg
     9181 gttaaatacg tgagcccaaa tcttccagca agtgactgca tcaaacgagc agtgcgattt
     9241 tttgcttctc ttcttaatat tgcttctgac cctagcagcc taaaaccagt tttcctagta
     9301 tacgacagct gacaatcttc cacttctaat tcatttctta tctttttgat atctgacagg
     9361 actgtccctt tagaaacctg aagaaaatct tgaaaatgaa aaacagaaac agacgttgca
     9421 gtcgaataaa ccatcaaata aagaagatct cttcgctctt gttcggtata cacagcttca
     9481 aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg ctcggaattt ctttatacgg
     9541 atacgggtat catccacata ttcggcaaaa ggagttaatt gtttgatcgc ctcgcttgta
     9601 tttacttcga tgatctctgc tgctttttct agactgattt ctcttggtgt tttcaggaga
     9661 taactaattt tgtagttcac agcgctgttc acctcctact tatattctag tatggtagcg
     9721 cttttaaatc gaggggtgtt tggtacaact tacagttgga ttttttttac gactgtaccg
     9781 aacgatttct atcgaatgga tacagaaaaa aagcaggtga ccaaatgtct cctgctttta
     9841 tgaattgatt tcgtccgatc aatgatgatc atgtttattt tttgaggtca gttcttcaaa
     9901 tttttctttg acatcttcaa ccgctttttc gatatctttt tcaaagtgat tgggctcatt
     9961 aggctttgtt tcctcttcta cagaatttga accagataaa gctgtttctt tattttctgt
    10021 ttcaccagag tttgtgccag ctaatgattc ttctttgtct tctgtttcac cagaattagt
    10081 tcctgctaaa gccacttctt cttctgtttt aggggccatt gaacctctta atcgtttgtg
    10141 tttttcaccg tcataatttg tcatgttggt tcctcctagt agttctatac tttttcatcg
    10201 tacaataaga cagtttatgc gtcaaaaaac aaacccgatt cttacaaata tgtcatattt
    10261 ttccttctga ttcttgaatg cttttgattc cttcttccaa aattttcatt tgtaagattg
    10321 tttcttcatc tttgatgact ttttctgcgc cattaactaa tgtctcgtaa atcccttcgt
    10381 agacacggct gtaatcacca acctcggaaa ttactttttc ttcatgataa ctcccttgtt
    10441 catctatata ggtcaataca ccataatgtt cgggcaaatc gataccaaag tcttcatgat
    10501 caggcatgta aaacattttc agatgttctt cttgtcgatc ttttgtttgt ttcacgaaca
    10561 tccctttttt tccgtaaacg acgaaacttg gacgttcttt gatgcggaaa taactggatt
    10621 ttatcgaaat tttcatattt ccatagaaaa aatccagatc gaagtaatca ttcattcgtc
    10681 ctttccctaa aagttgacgt acatcatagc tgatatcatc tggttcgccg aaataagaaa
    10741 gaacttgatc tactgtatga caagcatgtc catataaata gctgttcgca gtagaaaatt
    10801 catgcgtatt ttctggtact tccggtctga aataatcata atgcatttct acttctaaaa
    10861 tatcccctaa ttttccgctt tcgattactt tttggactgt caagaagtca gaatcgaacc
    10921 gtctgttttg gtaacattgg ataaatagac ctttttcttt tgctaatgca aataactctt
    10981 ctgcttcctt tgacgtttct gcaaaaggtt tctctactag tacattctta ccatgttcga
    11041 gtgccatttt tccatattca taatgaaatt gactaggtgt agtgatgaca actaattgga
    11101 tctcaggatc atcccaaaga tcagacagct gatcagtata ttcgatgtct tcgaatgttt
    11161 tccacactgg ttcagatggg gaaaaaattt ttttgatatt gattttgttc gtaagttttc
    11221 ttgaaaaagg caaatgatag cgatttgtac tctttccatt gccaagataa gcgattgtaa
    11281 gcatgagtgg tgcctccttt tattgatgtt ttcagtatat cctagaaaag cgcagaaagt
    11341 atttataata gctattctgg taaaaaagtt cagaaaaaca cttgttatca ttcggtttgg
    11401 tgaaattaat gttcaaagta aggataaaaa tcaaaaagat agtagcggaa ccaagtgttt
    11461 atcagtaaga tagaatttgg aggtcagatc atgcttattg aagaaaaact aacaaaacaa
    11521 gagttgttta ccacgacaga gaaacggatt gtcgattaca tccgtaggaa tatcgaagct
    11581 gcagtctaca tgacgattga agaattagca aaagctacgt atacttccca ttcagctatc
    11641 attcgtttat gcaaaaaaat gggattcagc ggatttaaag aattccgttt cgagctgggg
    11701 cgggaagttc atcaattgat tgctcagttc aatcaaacga ttgatcctaa ttttcctttt
    11761 tcaagtgaag atagcactca gcaaattgca aagaaaatgg cggatttatc gatccagtca
    11821 ataaaaaaag cacaattgca gattgaacat gaagatttag atcaaatcgg taaagtattg
    11881 aacaaggcac agcgaatctt tctttttgca aaaggtgatt ctcaaatcac ggcaaggaaa
    11941 ttccagaata aaatggtcaa gttgaataag tttttgatca tggctgaaga atacagtgat
    12001 tcttcttgga atgcagccaa tttgactagc gaggattgcg ccatttttat tagctacagc
    12061 ggtcgcatcc atcattatga acgaataatg acctatttga agcatgtaga ggcccctacc
    12121 ttgctgatta ccggaaatca gcattcagaa atggccaaac aggctgatat gtgtcttgtt
    12181 atatcgcagg aggaatatga ttttgctaaa gtagcgactt tctcctctca aattgccttc
    12241 gattacgtat taaatacgtt attctcagtg atatattctc aaaattttga agagaatatc
    12301 cgcaacttga aaaacaaaca agaattgatg caaaaaggcc ttcttaaaga atatcggaaa
    12361 acttaagaaa agatcctcat ttagtaataa gaaagatagt gtttgatagg ctctctttga
    12421 tatcatcata aaaacaggct gaagttttct ttttcaaacc tcagcctgtt tgtttacgga
    12481 acgattcgaa tagatatatt atagaaatga ggaatagcta acttctgttt cttattttga
    12541 acgattgacg aaaaagatat ataagaagga ggaaaatcag tatacatgca gctaacaatc
    12601 catacaggaa aaatcgtttg ttctcggata ctttctgtac ttctttttga acttcttttg
    12661 catgataagg aacccggtgc cctctgacaa gtaagcgatg actgttgacc atataaggtg
    12721 tacaagttag gagtgtcacg aaatcctgcc ctttttcgat tctcagatct tttgtatcag
    12781 tcggttctac cgtcttgatc tgatcgactt gataagcgag tgtccgtcca ttgatttcga
    12841 tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt gaacaacttt gcttgaggca
    12901 gtcctcgatg gctggaaata acggcatgtg tattttctcc ccccgtagga taagacgttc
    12961 cttccaataa tgaggcacct ttttctagca gcacagtatt cgtttcatca aaaattggca
    13021 aatggacatt gatctcaggg atcgtcaaaa taccgatcgt atgtttctca aaataggttc
    13081 tatccttttt cgcaggttct tttttcttag taaagggatc tgcacctgga tttccacctt
    13141 cttttgctaa ttgctggttt ttctttgtca tgttttcttg gattttagcc atgacttctt
    13201 cattttcttt gaccgcttgt tgctgataat gagagatgat ctgctgatcc agataattat
    13261 tcagcgcgtc gctaacaaat ggataagcta gtatccctat tcctgagaga agcaagaaaa
    13321 gcatcagcag atctagaagt ttccttttct ttggtttcat ttgtgttctc cttgtgatga
    13381 ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc gctgctcctt tttccaattc
    13441 ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa agtacagtcc agcaatcagt
    13501 agaaggacta ctcctgcacc gatatacaca tagattccct taccgcctgt tgaaggaagt
    13561 gtacctttgt gtttatttgg tattttttca ggagaaatca actgtcctgc tgttacataa
    13621 gattgttcat cgatagtaaa tgctacacgg tttgttaatg gaacatattt ttctggcgct
    13681 ttcgtttctt ccagatagta cgtgccatat tttagacctg tcacatcgat taaaccgtta
    13741 cttgtggttg taaaaaccgt tgctgattct ttcgccgata cccagctgac ggcttttgtg
    13801 gatgggtcga tcgataaata tttcgctgtg tcactatctt gatcacgaac gacgaattct
    13861 gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta ctttaacaaa tcgtttgccc
    13921 ccagtaacga catcgactga cggtggtgtt tgatcatttg tatgcccgtt atcgacattt
    13981 gcttggttag aaaatccttt ggttggatct gctttttcgt tcaaatgcat atagtaaacg
    14041 aatttcaatg taccgccagg agttaatgaa ggaatataat tcggatcaac tgaaaccgtg
    14101 aatccgtctg tttgctcagt gacagaataa tttactgggt cgatttcttt atttccatca
    14161 tataaggcat aagcatacgt tcctgaagaa tcattatcaa atgttaaagc agcgtcatga
    14221 gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc cttctttatc agcgatacct
    14281 aatgggatat tgacagaaat ctcatattga attttttcac cgattgcgac atcttttcca
    14341 ttcaattgag gtgttgtttt atcaacttta gacgtatcat ttttgatggt cttttctact
    14401 ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg tttgcttttc aatcatttcc
    14461 gcattatcag gagcttttac ttcttctaaa ttatattttc caacttctaa atgattaacg
    14521 atcaactggc ctttttcaat agatgcctcg gcaaagtcat tgttgccgat gtcataagaa
    14581 tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg tccaagtgta caatccatct
    14641 gtgacacttt ggatgtattt tttgacgctt ggtgttcctt cttctttaga aataataaat
    14701 tctgctccat ttagtgcttc gttttcggca gtaccgattt ttgtaacttt caacgttcca
    14761 tcattaccga ctgtattttt agggtagaga tggatagtat ctagttcttc tgtcccgtat
    14821 ttataagagc catctgcttg tttgatcatc tcatatacag ggaaagctaa aaccatgttt
    14881 gcggcagctg acactccgtc ttttggttct tctttgatcg tgtagactgc atctttccca
    14941 ttttgttttt taggtaaaga taaagtgaca tttccatcag catctgtcgt tcctgaagca
    15001 actggtgtac caggagtcaa agactggact gcttgttttg ctgcatccac ggacgctcct
    15061 ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa ctgaaaatga aatatcggct
    15121 aatccttggt attgatcgaa ttcgctcatt tctttcccgc tgttttggat taaaggatcg
    15181 ggtaaatcag tcattttctt tttatgaagg atgacttgag ctgctgtctc ctctgcaaac
    15241 actttttttg cgccgaagct gtttgtgagt aatggtaaaa taaggaaaag gactcctaac
    15301 ataacgttta tttttttatg atttttcatt ttttcactcc tgttcttttt ttgataaaca
    15361 accagattgc aaagcttgcg aataccatac caacaacgcc taaaagtgta tttttcgttt
    15421 cgtttgtttt tggaagaaat ggacggctat tttctttttt attgactact ttcaatatac
    15481 tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt aaacggaatt tctgtttcac
    15541 ttaaaatata accagaaggt gcttcgatct cttttaatcg ataacttcct gttttcaacc
    15601 cggaaattga aaaagcgcca tttttatcgg aaatcaggtg aagcgcatct gattcgttct
    15661 ttgtccactt gtatccgttg gctgtttgct ggaggtaatt tcctgctttg tttttaataa
    15721 ggaatactgc gccagctaat cgtttatctg gtttatttgt ctcgattttg acaaattgct
    15781 tactccctgt atatacagag actttctttg tactgacacc aaaaccaaaa tccagtgttc
    15841 cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct tagctgcatc tgataagaaa
    15901 tggtcagttt ttttccagca tatttttcca acgaagggat cgaaaataaa acatcaaatc
    15961 cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt ttctccattc gatgaaatcg
    16021 ttaaactatt aggtaaaaac gttaatgctt catccgcact atctgacaat tcgaactttt
    16081 gatagtcaag gatatttaca ggaattgtcg tatgcaagga ataagtgatt ttttcaccaa
    16141 tcgtaaaatc atttctaggc tcttctaata ccttctcaaa aggtggatca gggtagtcgt
    16201 tttcttcatt tttaggatac aaatggatag ttgaaagttt ttgattgttt tgtccatgta
    16261 caggaagaac aactaccata ttctctgcct tttctttgac gacttctggt actttggatt
    16321 caatgaaaag atacgcttta tctctttttt cttgatcttt agcggccaga gaaaaagaag
    16381 cgataccatc ttcgttgtta agagttgtag ttgttgcttc tgcggtaaac ataccggaag
    16441 acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac cgttttgccc ttttcccgta
    16501 gatggtaaaa agaatctgtg acatcataaa cttggaatgt gacaccattt aatcctcgat
    16561 acgtttgtaa taaagctttt tcttgtccgt catttggatg atttttcggc agttgcccgt
    16621 ttgggaaaag caatttgtgt aaagtaatct gaaccatttc tgtttctgtt tctgttgcta
    16681 cctgagtaaa agctggttta aatagtaaca agaagagaca catactaagc caaccaagtt
    16741 ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa agataccaaa tagaaaacgc
    16801 acaaccaatc atccctacta gatagattcc taaacggcca attcctcccg tttcaggtaa
    16861 tggtactttt gcctgattcg tgatgtctaa agaaatctgg ttgtttttgg ctcctggtga
    16921 cagaacagat tcatgatctt gtccatccac ttgaattgac ccatcttcgt gtataactat
    16981 agtaactggc tcttttagac cttggtatcc ttctggtgta aaagtttcgg tcagcgtata
    17041 agttccaggt tttaaatttt cgaatagaaa ggtatctgtt tcttgtccat cttttggcga
    17101 ttctaagtcc atttctggtc cctgcagtct gaattttgct ccttttaatg tctgtccgtt
    17161 gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt cgattttgat gagtgagcgt
    17221 ccatacatta tttgtcatct cggtattttt tcccgtgtaa atgatttttc catatttatc
    17281 gatttggaat tcataatttc ccggcgaagt atcatatccg gcaggaccag ctgtttcaac
    17341 gactcgatat ttaccaggtg tgagtgaatc aaaactggca agaccttttt cattcgttgt
    17401 ttggctgtca attggctgat aagtaccatt tgcttctttt ttctgtaggg aaaatgctgc
    17461 tcctttaaga tttatctctt tgtccgtccc ttgcatcgca tacttacgta ctgctacagg
    17521 aacttcaaca aaaggatttt caatagtcaa ctggatcgta tcatcggaag tagtgactgt
    17581 ttttccatca atggttaccg taccatcaga agcgatcttg atttcccaag tagtcttttt
    17641 gcttaatcca tgcccttctg gagcttttgt ttctgtcagc gtataggtca tttctttttg
    17701 caatttgaca ttttctggaa gagaataggt tccgtcgcca tgatctgtta gtaaagtatc
    17761 aatagaatct cctgttaatt tgaaaacagc gccaataaga tccttttcac ctgtagagga
    17821 atttttcgtt attttcaagt ttaacggtgt gaattgttta gtatttttcc atgtcattgc
    17881 atctatttgt tgagaatcgt atcctggtac aggtaattct ttgattgttt ggtaactgaa
    17941 tgcttgacct tgattattgt attgaggtag tgataatatc tcttgataac tttctccgct
    18001 atttgcagat aatttgtcaa tgtctgcacg ttcccacgta tttgttgtat ctttagctgg
    18061 tttaatgatt cgaatatatc cgtttttcca agctgcagca tttgtcgtat gttcccgttg
    18121 aatctcaaaa gtaacttgat ctggacgatc agctagattg ttgtcaaatt cttcccataa
    18181 ctttttgatg tgaagactga ctccgggagc tttagcagat ggtataccga attcagctaa
    18241 ttcattggta tcaatgtttg gctggaaagt tgtcctgccg ttcatttgat accagaaatt
    18301 tggatggaag tcctcatttt ctgtttggat tctcacttgg taatggattt ggatttcttg
    18361 gtcttttcct aaatagatct gattgctctt gatggtattt ccttctatgg aaatagatgg
    18421 agtgaccgtt gtaggacttg tccccacact cttgactgaa agtgtaccag gctgataaat
    18481 gaatggttct gcgattggat catttatttg tccattgctt acagttgctg agatctgtac
    18541 agcttttttg gctaggtatt cagagatatc tgttgcatga tcagctgatt catagtaaag
    18601 atcgcctttt tcatctgatg aaaccatttg tcgcatacga gactctactt ctgcttttga
    18661 gagaccagct gccggatcgc tttgaagttg gatgccaaga ccatgtattt cgattcctcg
    18721 ttctttgagt gccatcgctt ctccgatggt tgcgataaac gtactgtcga ttcttctgga
    18781 tagattgttt tggtcaggtg catcatagat tcttgagatt agagaagtat ttcccggccg
    18841 atcttgcgta ttagaaaact gagttccgta ataattgctg cttgattgtg cgtgtacccg
    18901 ctgtacttta taggaaaatg ttggtacacc atccgtcagc aaaacgatca cttttttatg
    18961 tccatttgga acggatagca tgcttcctgc atctcttagt gctttttgtg taaaagtacc
    19021 accatttgtc cgtgaaggtg taatggattt tacttgattt ttcactgaat caaatgaacc
    19081 catctgtact gcaccgttac tgtagctata tccttcgctt gagtagccga catatcccat
    19141 attgattttg tctgtgatac cgctatctgc taaagtatcg acaaaacgat cgacaccaat
    19201 ctttacttca ccgatccgat tattgtcgtt catacttcct gaccagtcta cgaccaatac
    19261 gagatcaaga ggcgtgatat ctttttgtac atttccacga gcattcaaat aaacatcaaa
    19321 caatccgggt gtacttgttt cctttgcata ctttcgtatc gcatagtctg cttgattacc
    19381 ggttcctcca tattcgatat acgaatgggt ccgatcatta ggatctccat tccaagaatt
    19441 tataccatcc cattgatttg ttccgttttt attgccttga tgatcaagaa catttgtatt
    19501 tcctgtaggt tgccaactgg ctttaggata agtgccctga tcatctgttg tatattctgt
    19561 aggaatcgtt tgattagatg gcacactcac agctggcaag gataatgtag gcaatgtaaa
    19621 tgggctgaca gaattttcgt tttcttttga tgattcttct tctatttcgt ctggctttgg
    19681 tactgacaca tctgtatctt tcttattttc tgtctcatca ataatgtcat attctttatt
    19741 atcgacgatc agcttgtttg cctggctgtg gtaaagtgct ggatcgattg gaaaactcag
    19801 atcgatagtt tgtttagtgt ttgatgagat ttccaacgta attttcccgc tatttacctg
    19861 ataagcatag ccatctcctt cttttctttc cacatcactt aagtaatttt cgactagacc
    19921 aatttctttt tctatctttt cagcactggt gttattcgca tttagcttga tgttcagtgc
    19981 tttttttcca gactgatccg aaatgacttc agctgaaatt gtttctaaaa ctgttggatc
    20041 atctgtagtg tctgctattg cagataatgg cacaaagttt gataaactca atattatcag
    20101 catgaaaata actttcagtt tcttccctgt ggtcgtcatt tttcccctcc tttttctttt
    20161 tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa caggcgttac ataattttat
    20221 ttattttaat atgataatta ttatacgaag ggcgctaaag ggaatcacac caattttcat
    20281 accaattggt ttcatttatg aagttgattc cttcgattaa tgagcgtttc attgaaagaa
    20341 aagggttgag ggagaaaaac atatgatcag gttgttggaa ttaaactata gaaagatgga
    20401 aaaaatcatt cgaattctgt ccatttctga caagtggatg actaaaaaag aacttgccaa
    20461 acgaattggt agttcagaat ctacatttgt aaggtatatc gaagagatca aaatccgctg
    20521 gggaacggat gtaacgattc aatcttcaca aaaattgggg tatcgtcttg aacatttgaa
    20581 tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa agttctacaa acagtcgttt
    20641 attaaatgag attattgtca atccgggtaa aacgattgag tattattgtg aaaaaatttc
    20701 catcagccgg tcttctttta cacgtaagtt gaaacaatgc aatcaaattt tagaaagcta
    20761 tttattaaaa gtgatcgttg accaaggttt tcagttaact agccttaaaa gtgagctcca
    20821 gttgcgtatc tttgttacct ttttcttttt aagttactac ggaagacgca cgttgccatt
    20881 tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag aataattgtc agatgaattt
    20941 gtttcaggaa gaaagcccat atgagcaatc tttctttatt atgtattttg ttgtgcatct
    21001 catgagagaa tcccaaggaa ctccatttga atggaaagct ggagactctt atgtacagtt
    21061 gataaatatc gataaaaaag acttcttaat cttgaaaaat ctcttaggaa aattgaagta
    21121 cagcagctat ataaaagtag tgaactattt tgtcaatacg attttttcag aattttcgga
    21181 gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat atgtcctact gttcgttttc
    21241 atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt caattattaa cgcaaattta
    21301 ttttttctcc cgtattttcc catacgatac gactcatttg acgatccgtg caacttactt
    21361 tgcacagcag atgcaacacc gccagatacg tctatatgct ggatttcgtg cagaacttca
    21421 attttattcc actctactga atcagaatct gatttattat ttatctagct atatcttttg
    21481 gatgcttatc ggtaatcctg aaattcacca ttatacttct gataaaaaaa tactgattat
    21541 cagcgactta agtcttcgac atagtcaata tattgaagaa tatatcagtg atattcttgc
    21601 tgtgcataaa attcactctg agacaactgc tattactgaa gatcagctgt cgaaatacaa
    21661 tcttctcgag tatgatctga tcgttacgaa tcaacccatt ttgaatgccc atgttcctca
    21721 tatattgatt gatgatagtg tgtcattcgc aaatgaagaa gaactcatcc gcttattcga
    21781 gttatgaata atcaaaaaac agctatgtat aaaccaccct gataaaaatt tcaggacaaa
    21841 tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat ttatagcgaa
    21901 agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc aaagtgttca
    21961 ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat tgaatggaga
    22021 aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca gccgatagtt
    22081 ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac ctttgccaat
    22141 agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc cccaaaaaga
    22201 aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg tgagaactgc
    22261 ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa aaagcaccgc
    22321 gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg tgctgtgagc
    22381 aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa
    22441 aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga tagcccttca
    22501 ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc atcttccatt
    22561 cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat aatagtcgct
    22621 ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc atgatctgta
    22681 tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata taaagcgcga
    22741 gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact atttttaagt
    22801 gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa
    22861 gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat caaattagag
    22921 ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc
    22981 tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg caatcctcct
    23041 gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct gacgaattct
    23101 ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg accagaacca
    23161 aatgttttgg taactttttt catcgttaat atttttttca tttttattaa cctcctattg
    23221 ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct agcattgcga
    23281 caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca aaaggcatcg
    23341 cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga acgcctacca
    23401 ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt ttgatccctt
    23461 gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac atgaaaatac
    23521 cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag gtcatatttt
    23581 gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac tgtagaccct
    23641 tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca ttgatcggct
    23701 gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac gtatctaggg
    23761 acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg acaatcgtca
    23821 aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct gaatcagcca
    23881 gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc gtgagttttg
    23941 gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg ttttttcctt
    24001 tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg tatttcagat
    24061 ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac aaaacaatct
    24121 cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca ttcgctagtc
    24181 ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc aagccatatc
    24241 gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc tccctatgat
    24301 agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc
    24361 acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat aaaaccttaa
    24421 catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa actaaagtga
    24481 ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga taaagtatta
    24541 tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt ttatgatggt
    24601 agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg
    24661 atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact
    24721 ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg atttgaacga
    24781 ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat gcgcgtgaaa
    24841 gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta taaggatatc
    24901 agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc catacaagga
    24961 aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt gacaaaagag
    25021 cagatatttg accggatctg gggatttgat tctgatacga cgattacagt tattgaagtt
    25081 tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga aatcaaaacc
    25141 ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag atattacacg
    25201 aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta tcttttttct
    25261 tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa cagatctgtc
    25321 attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa gatatcagca
    25381 aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca tggaacagcc
    25441 attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt ctaaaaatgg
    25501 tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag aattaaaaat
    25561 ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca gtggctcatc
    25621 tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg gcgttgccta
    25681 catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt ttcgcacgat
    25741 tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt attacttgtc
    25801 tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat ttgttgagaa
    25861 tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg aacatctttt
    25921 taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag cattgtcaga
    25981 gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga atgattctga
    26041 tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac aacttgttaa
    26101 accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata acgatgcaac
    26161 attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt tgctggataa
    26221 tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg ttaaccataa
    26281 agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag atcaagcaag
    26341 gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg gcggatacgg
    26401 gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga tttctgtatc
    26461 caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc ctaaaaaata
    26521 atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt tttgttgcga
    26581 ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt taagctgacc
    26641 acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc ttcaatgtgt
    26701 acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt cactttttca
    26761 acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt atgtttgctt
    26821 acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa tttttcgtct
    26881 gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc ttcacgtttg
    26941 accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc gacttctcca
    27001 gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat tgtatcatca
    27061 tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt atcagtctcg
    27121 ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt tgtattttga
    27181 aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc taatccagtc
    27241 cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc tgttgtttct
    27301 ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc tcctctatat
    27361 tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa
    27421 gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg attttcatct
    27481 gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc ttcatcagta
    27541 gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc aattactgat
    27601 aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc ttccatgtaa
    27661 attcctccta gcaaatagat ttatgattgg tatattgtta cttataaatt tattctatag
    27721 gagacgattg ttttctacaa atatttagat tggaatttga ttaaaacaat ttaattgaaa
    27781 tttgaaaaca tttatgttaa tgaaagtcat attctaataa aatgtcattt gtttctatat
    27841 gaatttttat gaaaaatttc atataatttc cactcgttgt gatggtttaa tttatccaat
    27901 ccatcaataa gcaaacaact tatataaaaa aggattaatt ctcaatataa ttgctcctac
    27961 catagaataa atcttcatgt aaagtaatga agtaccaact atttctaata cttctttaag
    28021 aattatccca acaatatcgt tagacaaacc attattgaat aaatggagag ccaatcgatt
    28081 ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag agcctcctgc cattagaacg
    28141 aaccaaatcc atttcattgc tcatgaagta accacatagc taaaaagagg ataaatcaat
    28201 taaaagatca aaagaatgac tgcactaata aaataactaa aaatgtatct tcttacttca
    28261 tatcttttag ctagtactta gttaaaaatt tgcttcctaa gatcaagaca aactgtctat
    28321 cttcatttga tgaaatgcgg taaaatatag aaaatgaaca ctattggagg gaatttgtaa
    28381 tggacagcaa aactagaaaa caactgcaag aaaagttaaa gcaacgtaca cacttgctcc
    28441 cttccgtctt tcaacagttt ttagaagaaa atcgttcttc cctttcatta ggcagtcgct
    28501 atgaatacgc aaaagatttt catctgttta ctgattattt gagaaaagta ttttctgaag
    28561 aagtggatga tcagctgatt atccatttag aaaaacaaga ttatcagaat ttcttagcta
    28621 aaatctatcg acatactcgt tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt
    28681 ttgaaaatag ctattacggg atttcaagga agcaatattc attaaatcat tttttacgat
    28741 ttttgtatca aaaaggactg attgaagaag agatttctct tttgggtaca agtttaccgg
    28801 aaacaaccaa agcagctccg cgatatatcg atgcagaact tttcaaagat tttgagcatt
    28861 tgtttatccc gattgaaggc tttcaaacaa gcagagaagc tagctttgaa gaaaagaata
    28921 gaccgaggaa ccttgcaatt actgccattc ttctgtattg cggattaaag atccatgaag
    28981 tagtagaact taaacgtaaa gatgtatctt tgaccaagca gattcttaat ctgaatagaa
    29041 aagaaaacag actgaataaa gtaccaatac caaaagaagc gatgccattt ctggagaact
    29101 attttaagtt aacagcacaa aatacggacg aaaattcttt tgtttttcgt tcctcacatg
    29161 atcagcaaat ttctcctcgg actattcgcc aaattttgag caaaattaca gtatataaaa
    29221 acgtatcgcc agaactatgc agaaaaacat atcgttatta tgcagaacta tatttagatg
    29281 atgctgacgc ggtaaattat ttatgtggta atcgatacct ctcatcccat tcttttcaag
    29341 atatctggga aaagatggtt gatttttctt atcacgaatt agcggcattc gttcgggttt
    29401 cttccatata aaacaaatgt atgatctttt tttcacaata aactatgtta ttatataaag
    29461 gattatgtta cgattatctt gaatttgtat attacttaac aaaagataat ggagtgtgaa
    29521 tgatggagac cataacgtat tcccctttga acttatttac gaattttcaa gaagctgcag
    29581 ctaacttttc cgagaccccg attatctttg atgagccact ttcagctttc ccagaattag
    29641 gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt gaaacgggct tatcaattgg
    29701 ctcatcttgg agtgaaatcc ggagacaaag ttattattta taagagttcc gcttttgaca
    29761 cataccttct agctgtttct gtgtcttacc ttggtgcagt gcctgtcatg acttcttatc
    29821 atctaccaac aacaacaatg gaagtattta tcgatcgttt agaagaccct ttcattttat
    29881 ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa tggaacaaaa tcaaaacaga
    29941 ttcctattct ttatttaatg gaacaacctg ctttgtctgt ttctcaatca tttttagaca
    30001 aaaatgaaat ttcttatatg acccatactt ctggtacaac tggtatccct aaacttattt
    30061 gtcattctgc tcattctatg ggatggcgga cgaaatggca aaaaacagtt ttcacaaaaa
    30121 tttcggaaaa gaaattaatc gcttttcaca tctctccagt gcattccaga ttcaatattg
    30181 gtatctcttc tttgatgtcc atgggatttc ctatgatgcc tttagcaaac gcacaaagtt
    30241 caaaagttgt tcatatgttg gaagctcacc ggccaattgc actagagacc catccgaata
    30301 actttgttca gtggcgtttt actgctaagg agcatccaga agctttttcg ggtatccgat
    30361 actatcactc cacttttgat gctataaaca accagacaat gaagacattt ttgcgaacgt
    30421 cgatggcaca ggatgcggta tttcttcaag tatatggcca aagtgaatgc ggaccaatga
    30481 ttcttaaggc tcatacacta gagagtttga aaacaagtga tgcaagagat atgggcgttg
    30541 gactggaaga catgacaagc gctcggatta cagattcagt tgggaatgtc ctacctgaaa
    30601 acacagatgg tcatatacaa ttactgtcta aaggacgggc tttgacttat tacaaggaag
    30661 atgcacggtt ccaggaaaat gtttatggcg attggtggga tagcggagat tacggtttta
    30721 tggataagca tggccaccta tttttgaaag atcggcaagt ggatttgatc gaaacgatca
    30781 atagtacatt agctattgaa gactttttat tagattcgtt agattttttg gcagaagtag
    30841 tgattgtaag ggataaaaat cattctccac aaccgattat tgctttagct cctgataaag
    30901 aaatggattg gaatcgatgg tgggagcaag tccatgaact tcctcgcttg aacgtcccta
    30961 tcatccgtga ttttgatgca atcccgcgaa cagctacaat gaaagtgcaa cggcttcaaa
    31021 tcgagaaaga attaaaatca caaaagtcct aactatttaa ggaaagaaag atccattttg
    31081 gtctttcttt tctttttgtt tttttaaatt ggatgagata aaaatatgcc aattggctgt
    31141 ttttcttttt tgaaaatttc gttatactct tattcatgga ggaaaatgtt atgaaaagaa
    31201 tcttgacaat tggcggttct gatccatttg ctggtggagg aatccaaaca gatttaaaaa
    31261 cttttgagaa ttttggtcta ttcggactaa gcgccttgac ttctataggg gcactagata
    31321 ttaatgagcg atttatgctg gaatctatat ctttggaatt gcttaaacga cagttagttt
    31381 cgattgataa aatgacttca ttagatggta tcaaaattgg gttattgaat tcaagtgaag
    31441 caatcatggt tgttcgagac ttcataaaaa caaaacaaaa tatcccaatc attttagacc
    31501 ctgttttggc attcaaagaa actgaagctg tgatcaatca attgtatatt tcgcagctca
    31561 tcgaagaact ttttccactt gttacgatga ttacgcctaa tttggcagaa gctgctttac
    31621 tttctggtca agctcttccc tcttcgttaa cagaaatgat ccatctcgct aaagaactca
    31681 ctgcttcagg aacaaaaaac attgtgatca aaggcggcac aaggatggct ggagatgaag
    31741 ctgtggatct tctctattca attgatcatt atgaggtatt ttctcataaa aaaatcgaca
    31801 ctacaacagt aaacggggcc ggttgtgcct tttcttctgc aataacagcg aatcttgtgt
    31861 taggggcaga ttttaccaaa gcagtagcag aaagtaaaaa atttgttgcc cattgtattt
    31921 ctaatggcgt gctgatgaac gatcataccg gaagtgtctg gtctggcggt attcaaaaag
    31981 gaggaatgag aaatgaaaac taagcaatta acgatctatg ctatgctgat tgcgttgaca
    32041 gttgcccttt cattaacaat tttgatccct gtaccggcta cgaatggatt tgtgacatta
    32101 tgcgaggccg ggatctatac cgctgctttt ctttatggtc caacgggtgg attagttgtt
    32161 ggtgcttcca gtggtctatt gatcgatttg atttcaggat atccgcagtg ggcagttttt
    32221 tcattagtga tccacgggtt acaaggatat gttgctggtt ctttcagcaa atcttctact
    32281 cgtatgtggg gattagggct tttattagga agcttagtta tgatcatcgg ctatggtatc
    32341 gccggttgga ttttatatga ttggccggct ggagtggctt ctataccagg aaacatcttc
    32401 caaaatctga ttggaatggg acttgccttt cctataactg caagtcttca acgtattcaa
    32461 gcacggaagc aatattaatt gaaaaagaag gttttagaaa atgaagttgt cagaaaaaga
    32521 attaaaagac caattgactg aaatagttaa tgacatattg gctgaggcac acttgaaaaa
    32581 aggtgatctt tttgttttag gatgcagcac aagcgaagtc gttggaggac atattggaaa
    32641 aaattccagt gcggaagttg gtcaatggat catccgtacg ttaaaagagt tgttggatcc
    32701 taaagaaatt gcgctagctg tccaaggttg tgaacatttg aaccgagcat tagtagtaga
    32761 acgaacagtg gcagaagcaa aaaatttcga aattgtctct gttgttcctg cactacatgc
    32821 tggaggtgct tgttctgttg ctgcttttga ccagttcaat gatcctgtcg aagtggaaca
    32881 cgtagttgga caagccggta tcgatatcgg tgacacatca atcggtatgc atatcaagca
    32941 tgtccaagta cctgtcagac ctcgtttgaa aacccttgga caagctcacg tgacagccct
    33001 tcgctctcgt cctaaatata tcggtggtcc tagagcgaat tattgaaatg agtaattgac
    33061 agagcaatct gaaaaacttc ctcgcaaagt ccaactaatg aactttaacg aggaagtttt
    33121 acttgtttgt tttatataac cgagcttcat aaggtgctag tatgccttta gcttcaaatt
    33181 ctccgctaga ggaatttgac aagagtaatg tccaggactg atctgcttct tttggaagtt
    33241 catagtcagc aagtgattct gttaagttta ccataataag atattgttca tcttctaaat
    33301 atctttcata gacataaagt tgtggatgtt catgaagata ttctttataa gaaccgtata
    33361 tcagaccttt attggtttgt ctaatgcgga tcatgttctt atagaattgc aggaccgact
    33421 ctggttcata ttcttgttgt tctacattga tcgttttggt atttggatta gtgatcagcc
    33481 aaggttcatg tgtagaaaaa ccagcatatt gttccgaagt ccattgcatg ggtgtcctgc
    33541 tgttgtctct cgttgtttca cgaatgatat ccaatgcttc ttctcttgtc ttaccttccg
    33601 caagcagcct tttataaagt cttttcgaat ctactgcatc aacttgatcg atcgattcaa
    33661 agggcatatt cgtcattccg atttcctgtc cttgataaat aaatggcgta ccttgcaaaa
    33721 agaaatacat catagcgatt gcttttgcag acatagccca aaaatcggga cgagtatctc
    33781 caaacactga aacggcacga ggaacatcat ggttttccat atataaagcg ttccatccga
    33841 taccatccaa tgaagtttgc caagcgctta atgcatgttt caatttaagg acatctaatt
    33901 gcccttcttt ttcttgctgc cagatatgga tatgatcaaa ttcgaatatc atatcgaaat
    33961 aaccgtcttt ccctacccat tgtggtcctt cttctgcagt gacaccactt gcttctccga
    34021 cagtcatgat atcgtactct tcaaaaacat gtttcaaatc ggtcaaatgt tcttcgattc
    34081 ctgagacatt ttgaaagggt gagaaagggt tctctgtggc ttttactgaa tattcatctt
    34141 tttttacatg agaaatcgca tctaaacgaa atccatctat ccctttatcc aaccaccatc
    34201 ggatcatatt gaatagttct tctttcaatt tctcgctttc ccaatttaag tctggttgtt
    34261 cttttgcaaa aacatgcaga taatactgtc cagtttcatg agaatactcc caagcagaac
    34321 cgccgaaaat cgatgtccaa ttattcggtt ctttgccatc taccccgtcg acccaaatgt
    34381 aataatcgcg atacgggctt tctttagatt ttttcgattc ttcaaaccat tgatgctgat
    34441 ccgaactatg gttgatgaca agatccataa tcactttgat ccctaattca tgtgcttctt
    34501 ttagcaataa atcaaaatct tccatcgttc cgaatctctc ttctatatct tgatagtcac
    34561 ttacgtcata accattatcg acaaatggag attgatagat cggattgatc cagataaagc
    34621 cgataccaag atctttcaaa taattaagtt tttcacggat cccattgatg tctccttgac
    34681 catcgccatt gctatctttg aaactagcag gatagatttg atagcctact gcttctttcc
    34741 accatttttg ttcttgtatc atatcgtttc actcctttaa cggtccctct tgattataac
    34801 cgattctatt ttagaagtat ccttgttacc ggtaactaga aacgaaaagc tcaacgaatc
    34861 tgctaagtgc gttcgttgag ctttatttta tcatagatag cgggcaatca gttgaatcat
    34921 ttgcttggga aattcattca agtcagtaat atctacaaaa cgactttcac cgtagatcga
    34981 cttgatttct tctttgtctt ggccaatagc tgcggagaca aataggattc cttgacgttc
    35041 atattctttc aaaacagact ggatatcttc ttttgctttt ttgcctgtat aatccggcaa
    35101 cgcttttggc tgaccgtcac taatattaag caacagtttg gttgttgcgg attgtctgtt
    35161 taatttttct gctgctaaac gaagtgcagc accatcacga ttgttttgcc ttggtttcat
    35221 agtgactaat ttttcattta accattgaaa tccgtcttca aattctttat aagaaaacaa
    35281 agaagtcttt tctcttgacg aacggtctgc tgtatctccg taaatcaaca atgggatatc
    35341 gactttctta gaaaattcgg caatggcaat cgctgctttt tttgccgctt cgatccgatc
    35401 atcccgtatc attgacccag attcatcgat ccgtacagct accgctaaag aaggttgctc
    35461 atgtggcggg ttctttttgt caaaatttct caaatcacca tatgctactt tgcttgcatt
    35521 gaaccgctga ccttcgtatt ttcctttttg gtacgtctct gtttgttcat tatccagtaa
    35581 ctctaaaatt tgccggctaa gtgattctac tattggcatt acttgcatgt gtagctcacg
    35641 tgcttccggc gtttttcctt ggaaagctgg tcggtgaacg atgagacctt ctttttcatg
    35701 aatcgtttcc ttcatgattt gtcgcgcttc tttattcagt tgcttcttga tttccttatc
    35761 atgtgcttcc atgtcagaag cagacatttc ttgagaatca tctcctgttt gttgttcttt
    35821 ttgatttttt ccttcgatat cgctactgcg ttctttttgc atttgaggag gaatctcgtt
    35881 tttttctatt gtttcttctt gtttctcttc tccctcattt ggttcttcag cagacaacga
    35941 tttcttttca tctaagcgca gaatttcttc agcattttca gtgatgtttc gttcatttgc
    36001 ttcttcggcg ctaatggttc tttcttttgt atgctcagat tgaggatgac cccatttttc
    36061 ttttctcaat atgtcctgaa gttccagtag caatccactg tcagacaaag cttgttcaag
    36121 aattgccaat tcatctgaat cagtggataa tttatacaaa acttttggat aaatcgactg
    36181 gagtaaatct tgtccttgct tataggtatt tgcccaataa aaataagaac gcattcctgc
    36241 tacacctttg atggcgttag cttttgcagt ttcatccaac aaacggataa tctctgccat
    36301 ctttaagaga agttcttgtt tttggatcat agttttcgcc atcgcacgtt ctgccatgac
    36361 ttctaaggca ggaagatcca ttttttccgc atgttgcatc cgatctctta aagattcatt
    36421 taatgggcga tttccttggt aatttcgatt tgtagtgatc acaatgacgc aatctggatg
    36481 gcgacgaatg attcctgtcg gtaaattcaa catcccattt ggttcgagcg ctgagttaag
    36541 tgccacaaga acagaggcat ctcggatgac agtcggctct tgaatctcta ataaatagcc
    36601 tttttcaatc gctcgaacga tttcagaagg gtaatagctg taatggattg catctggata
    36661 atcagcttcg atcctttgaa ctagctctgc taatttttct ttagcagaaa aaggatcaat
    36721 gccgtaatgg ttctggatca gtgtcagtac agcttcaagt gtatctgttt ggtaaatagc
    36781 ttctagtaat tcttggtctt gttccttatt agaatctact acaggtaata aggctccgaa
    36841 aacatctgat ttgtccatat ctgcaaaaca ggtcactttt gtatagggaa gttgcagatc
    36901 agcagatagt gctttggcta attgtgtctt ccccgacccg gcatctcctt ccaaaagtac
    36961 gttactgatt ttcatttcag gatcttgcca attccctttg atttcggagg cgatccgcca
    37021 ttcttctgca cttgtccgat ggcttttcgg tttcttccag atcatttgct tttctagccg
    37081 tgaaaaggtt cggttttctg ttagtgagaa attctccatg cttctcttcc ccttttgatc
    37141 atctctgtct attaatatcc tagctcatct aaaagacgtt ttaaaatacg gaatcgttcc
    37201 ccaatcagtt ctccgtcttt ttgtagtgta tcgtaataca aaatgatgtc ttcatcgatc
    37261 tttggatttt tggtatctac ttctaccgtc attcggttcc cttgtaatcc aatgatgtct
    37321 acgactggat tttctggatc ataaaatttt tcataacgat aagcatcttg gaagtataaa
    37381 cttccttgcg cagctaagat cgctaaatct aaaatacgtg tgattggtaa ttcttccgac
    37441 tgtcgtgacc atttttctcc agtatgtcgc cataccttgc ctgagatatc gacttttcca
    37501 cgatcattcc actgagcaag gcccagtgaa agcccttttg catccatatt taatgcattt
    37561 cttccatcaa ctttatcata gtcttctaca acgattactg gtttatgttt taaatttgtt
    37621 ggtatttcca ttattttaac tcctcatcct tttattttta gtaaataagt aaattagtag
    37681 tttactcatt tactaaacta tatgcgacat tatagaggat tgaagtgagg attgcaaaga
    37741 aaaagagctt cattgataga aataaaaaca ccatctcgtt cttttacacg ctcattagta
    37801 acatgtagaa agtccaagat gatgttggaa tattttggct ctatactttt tgatgttggt
    37861 gaacttcacc aacatctttt ttgtattctt aattgtcaaa ttaagttaga cagatcatca
    37921 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
    37981 atattatttc gtttagatgc gacagattga ataaaaaatt catctactga attgaaatcc
    38041 atagatttca ataaaccatc tctacgtaac aatccgttag aattctcgtt taggcctctt
    38101 tgagacggcg ttcctggatc agcgaaataa atggcaatat cattgacatt actgatctgt
    38161 ttccaatttg aaaattcctt tccacaatca aaagtgatgg atttgaatag gtttttcggt
    38221 acagattcaa accattgatt taattttttt tcaatatcaa tcgcttgtct accacacggt
    38281 ttcaatgtga tgataacttt tgataatcgt tcaactaagg taattacagc acttttatgt
    38341 ttcagaccta cgatagtgtc accttcaagg tgaccaaact catttgagaa ttggctataa
    38401 tccttttcac gttcatgaat agagcggcgg aaagtttgtt ttccacgtct ttcttgatgt
    38461 ccattcggtt tacgcttgcc tttcatcggt aagtcagaag aatcaaatag cccctttttg
    38521 aacatacgat aaatggtacg agcagaacaa ctaataggaa acgctgcacg accaacaatc
    38581 acatcgggtg tccatccttg aacaaccttt ctttgaatat attctgattg ttcctcgggt
    38641 aaaacaagcg gacgtctacc acagtttgat ttgtttttct tatactgttg ataatattct
    38701 aaggctgatt ttccttgctt gaaga
//
LOCUS       DACCWV010000009        38005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCWV010000009 DACCWV010000000
VERSION     DACCWV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.009"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            39..3317
                     /locus_tag="IVW84_000450"
     CDS             39..3317
                     /locus_tag="IVW84_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7916677.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3858..5765)
                     /locus_tag="IVW84_000451"
     CDS             complement(3858..5765)
                     /locus_tag="IVW84_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7916678.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5895..6770
                     /locus_tag="IVW84_000452"
     CDS             5895..6770
                     /locus_tag="IVW84_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7916679.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6850..7824
                     /locus_tag="IVW84_000453"
     CDS             6850..7824
                     /locus_tag="IVW84_000453"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7916680.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8013..9149
                     /locus_tag="IVW84_000454"
     CDS             8013..9149
                     /locus_tag="IVW84_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7916681.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9286..9630
                     /locus_tag="IVW84_000455"
     CDS             9286..9630
                     /locus_tag="IVW84_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7916682.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9901..11187
                     /gene="efmA"
                     /locus_tag="IVW84_000456"
     CDS             9901..11187
                     /gene="efmA"
                     /locus_tag="IVW84_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7916683.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11325..11924)
                     /locus_tag="IVW84_000457"
     CDS             complement(11325..11924)
                     /locus_tag="IVW84_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7916684.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12328..13449
                     /gene="mnmA"
                     /locus_tag="IVW84_000458"
     CDS             12328..13449
                     /gene="mnmA"
                     /locus_tag="IVW84_000458"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7916685.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13695..15116
                     /gene="arcD"
                     /locus_tag="IVW84_000459"
     CDS             13695..15116
                     /gene="arcD"
                     /locus_tag="IVW84_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7916686.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15130..16248
                     /locus_tag="IVW84_000460"
     CDS             15130..16248
                     /locus_tag="IVW84_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7916687.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16308..17600)
                     /locus_tag="IVW84_000461"
     CDS             complement(16308..17600)
                     /locus_tag="IVW84_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7916688.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17750..18310
                     /locus_tag="IVW84_000462"
     CDS             17750..18310
                     /locus_tag="IVW84_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7916689.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18439..18816
                     /locus_tag="IVW84_000463"
     CDS             18439..18816
                     /locus_tag="IVW84_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7916690.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18749..20821
                     /locus_tag="IVW84_000464"
     CDS             18749..20821
                     /locus_tag="IVW84_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7916691.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21032..21979)
                     /locus_tag="IVW84_000465"
     CDS             complement(21032..21979)
                     /locus_tag="IVW84_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7916692.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22225..22689
                     /locus_tag="IVW84_000466"
     CDS             22225..22689
                     /locus_tag="IVW84_000466"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7916693.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22751..23794)
                     /locus_tag="IVW84_000467"
     CDS             complement(22751..23794)
                     /locus_tag="IVW84_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7916694.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFGRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            24145..25008
                     /locus_tag="IVW84_000468"
     CDS             24145..25008
                     /locus_tag="IVW84_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7916695.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(25137..26759)
                     /locus_tag="IVW84_000469"
     CDS             complement(25137..26759)
                     /locus_tag="IVW84_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7916696.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            27008..27529
                     /locus_tag="IVW84_000470"
     CDS             27008..27529
                     /locus_tag="IVW84_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916697.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(27715..29445)
                     /gene="spxB"
                     /locus_tag="IVW84_000471"
     CDS             complement(27715..29445)
                     /gene="spxB"
                     /locus_tag="IVW84_000471"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7916698.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            29610..30428
                     /locus_tag="IVW84_000472"
     CDS             29610..30428
                     /locus_tag="IVW84_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7916699.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(30531..30728)
                     /locus_tag="IVW84_000473"
     CDS             complement(30531..30728)
                     /locus_tag="IVW84_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916700.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            30897..31475
                     /locus_tag="IVW84_000474"
     CDS             30897..31475
                     /locus_tag="IVW84_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7916701.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(32520..33023)
                     /locus_tag="IVW84_000475"
     CDS             complement(32520..33023)
                     /locus_tag="IVW84_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7916702.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            33382..34407
                     /locus_tag="IVW84_000476"
     CDS             33382..34407
                     /locus_tag="IVW84_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7916703.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            34730..35608
                     /locus_tag="IVW84_000477"
     CDS             34730..35608
                     /locus_tag="IVW84_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916704.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            36084..36200
                     /locus_tag="IVW84_000478"
     CDS             36084..36200
                     /locus_tag="IVW84_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7916705.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            37128..37466
                     /locus_tag="IVW84_000479"
     CDS             37128..37466
                     /locus_tag="IVW84_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916706.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            37560..37790
                     /locus_tag="IVW84_000480"
     CDS             37560..37790
                     /locus_tag="IVW84_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916707.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT        12246 a         6721 c         7739 g        11299 t
ORIGIN      
        1 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
       61 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      121 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      181 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      241 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      301 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      361 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      421 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      481 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      541 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      601 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      661 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      721 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      781 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      841 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
      901 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
      961 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1021 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1081 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1141 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1201 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1261 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1321 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1381 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1441 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1501 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1561 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1621 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1681 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1741 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1801 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1861 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     1921 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     1981 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2041 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2101 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2161 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2221 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2281 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2341 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2401 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2461 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2521 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2581 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2641 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2701 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2761 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2821 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     2881 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     2941 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3001 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3061 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3121 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3181 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3241 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3301 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3361 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3421 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3481 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3541 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3601 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3661 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3721 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3781 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3841 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     3901 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     3961 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4021 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4081 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4141 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4201 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4261 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4321 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4381 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4441 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4501 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4561 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4621 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4681 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4741 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4801 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4861 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     4921 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     4981 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5041 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5101 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5161 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5221 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5281 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5341 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5401 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5461 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5521 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5581 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5641 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5701 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5761 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5821 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     5881 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     5941 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6001 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6061 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6121 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6181 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6241 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6301 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6361 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6421 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6481 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6541 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6601 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6661 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6721 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6781 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6841 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     6901 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     6961 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7021 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7081 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7141 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7201 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7261 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7321 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7381 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7441 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7501 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7561 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7621 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7681 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7741 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7801 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7861 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     7921 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     7981 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8041 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8101 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8161 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8221 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8281 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8341 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8401 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8461 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8521 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8581 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8641 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8701 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8761 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8821 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     8881 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     8941 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9001 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9061 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9121 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9181 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9241 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9301 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9361 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9421 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9481 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9541 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9601 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9661 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9721 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt
     9781 aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa
     9841 tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt
     9901 atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta
     9961 tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat
    10021 ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt
    10081 cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct
    10141 cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga
    10201 acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc
    10261 gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct
    10321 cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg
    10381 ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat
    10441 gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct
    10501 cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa
    10561 ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg
    10621 ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt
    10681 ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg
    10741 atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt
    10801 atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac
    10861 tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg
    10921 gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac
    10981 tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt
    11041 ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt
    11101 ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa
    11161 gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac
    11221 aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga
    11281 gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac
    11341 ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt
    11401 agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg
    11461 ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg
    11521 agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct
    11581 acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa
    11641 taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt
    11701 tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg
    11761 tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac
    11821 tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat
    11881 aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc
    11941 ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa
    12001 ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc
    12061 acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat
    12121 tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac
    12181 tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag
    12241 aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca
    12301 gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta
    12361 gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac
    12421 gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg
    12481 gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac
    12541 tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac
    12601 cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca
    12661 ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa
    12721 gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat
    12781 caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta
    12841 ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct
    12901 aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg
    12961 agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga
    13021 caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc
    13081 ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta
    13141 tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa
    13201 atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt
    13261 tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc
    13321 gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga
    13381 atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa
    13441 tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt
    13501 cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg
    13561 aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa
    13621 gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag
    13681 tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc
    13741 tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt
    13801 ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt
    13861 tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg
    13921 gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt
    13981 tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat
    14041 ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta
    14101 tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa
    14161 tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat
    14221 ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg
    14281 gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt
    14341 gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag
    14401 aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt
    14461 cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta
    14521 tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct
    14581 tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga
    14641 tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg
    14701 ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct
    14761 tagttactga ttatgcctat caatttgcct attctttatg tacagcggca attttgattt
    14821 gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac
    14881 aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat
    14941 tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg
    15001 cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt
    15061 gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg
    15121 gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag
    15181 aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt
    15241 ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac
    15301 tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc
    15361 gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta
    15421 tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta
    15481 tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa
    15541 gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc
    15601 tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa
    15661 atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct
    15721 ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc
    15781 aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga
    15841 cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc
    15901 ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac
    15961 aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa
    16021 gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa
    16081 gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga
    16141 agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg
    16201 gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg
    16261 caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga
    16321 gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc
    16381 tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa
    16441 gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc
    16501 cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct
    16561 tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag
    16621 ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg
    16681 atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg
    16741 tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca
    16801 atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga
    16861 ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata
    16921 ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt
    16981 tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa
    17041 aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc
    17101 ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga
    17161 acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata
    17221 taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg
    17281 caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt
    17341 cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa
    17401 gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac
    17461 gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat
    17521 gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt
    17581 tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt
    17641 tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg
    17701 tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag
    17761 aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt
    17821 tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta
    17881 taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga
    17941 acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat
    18001 ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat
    18061 cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat
    18121 cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag
    18181 ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa
    18241 agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac
    18301 tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt
    18361 ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct
    18421 gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg
    18481 tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa
    18541 gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc
    18601 aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa
    18661 gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc
    18721 atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag
    18781 cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga
    18841 aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga
    18901 agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa
    18961 actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc
    19021 tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact
    19081 tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat
    19141 ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga
    19201 atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt
    19261 gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca
    19321 gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca
    19381 aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa
    19441 tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag
    19501 caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta
    19561 tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac
    19621 attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga
    19681 cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag
    19741 cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa
    19801 agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc
    19861 acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt
    19921 tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg
    19981 taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac
    20041 tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc
    20101 catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca
    20161 aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat
    20221 caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca
    20281 attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa
    20341 agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga
    20401 aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt
    20461 ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg
    20521 tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc
    20581 aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa
    20641 aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa
    20701 agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca
    20761 agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta
    20821 ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta
    20881 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg
    20941 gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat
    21001 ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct
    21061 tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt
    21121 tcccaatata tttcccgatg attccaagac ataacaattg gattccacca atgaacagga
    21181 agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa
    21241 taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa
    21301 tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc
    21361 agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg
    21421 ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta
    21481 ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt
    21541 ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg
    21601 caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga
    21661 tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg
    21721 tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat
    21781 ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg
    21841 ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt
    21901 tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga
    21961 caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat atggtttaca
    22021 ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt aaaagaacag
    22081 gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt atcaaacttt
    22141 taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga ggaagttcca
    22201 gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt tttatggtta
    22261 gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga aagcgaatca
    22321 gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt tgcgggaaaa
    22381 agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca gcaaaatgtc
    22441 tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga agtagccgtt
    22501 gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac caatccattg
    22561 ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac aggatatatg
    22621 ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga aggatatctg
    22681 caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca actgtttttt
    22741 catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact gtttggagaa
    22801 gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat tttgcaaagt
    22861 ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg ataaaatgtg
    22921 ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa aagatcacaa
    22981 accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca gttggcgttt
    23041 caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca acaacaacga
    23101 gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa ttgtcaagta
    23161 agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa agtgcacttt
    23221 tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc aacatcatca
    23281 cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct ttcgcatgga
    23341 tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct ccagccatca
    23401 tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca ccgatgatag
    23461 tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga gcaatcgttt
    23521 gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg ctttcagcaa
    23581 attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat aaaataccca
    23641 ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga agcctgccga
    23701 aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc gctgaatcct
    23761 ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc gtcaataaat
    23821 actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa gaacaagagg
    23881 tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca gaagtagctt
    23941 ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
    24001 tatttcttga agctgaccac ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa
    24061 taaaccggtt aaagtgtgtc tagcgcaaga gatttgctat aatagacgat aagggtaaaa
    24121 tgtaacgaga aggggttgaa aaatgtgtat caaattgtaa cagattcttg ttgtgatctg
    24181 ccataccatg tattagcaaa agaaaaagtt gattttttat caatgaatct ccaattgaat
    24241 gatcaagagt tagtagatga cctcggaaag acattcaatt atgaccgcta catgaatcag
    24301 ctgaaaaatg gtgcgatgcc tacgacatca caaatcaatg ttggccgata tttagaattt
    24361 ttccgttctt atgcggaaaa acagattcct gttttatatg tggctttttc cagtggatta
    24421 agcggctctt acagtagtgc gcttcaagcg gttgaattgc tgaaggaaga atatgaagac
    24481 cctgagatct atgtttttga tacaaaagct gcaagtattg gggaagggct tttagtattg
    24541 gaagctgtaa gattgaaaaa agaaggtaaa agcatcgaag aaaccctaga gtggctggaa
    24601 agtaattatt tgaaagtcca ttcatgggta acagtagacg atctgaaata tctagaacga
    24661 ggcggtagga tttccaaagc aagtgctact cttggcagtc tattgagtgt aaagcctatt
    24721 attacggtgg ataaagaagg aaggctgacg aatgttgaca aagttcgagg aagaaacaag
    24781 tcgatccaga caatcgtcaa tgagacggta aaaacaatcg ttgcaccact tgatcaaatc
    24841 gtttatgtgg cttacgctgg ggatctagag agcgcggaga aggcaaaacg gcttttggaa
    24901 gagcagataa agatgaaaga tgtgaagctt tatcccttag gaccaacgat cgccagtcac
    24961 acaggctatg gctgtatcgc gattttttcg atgggaatca gtcgataaaa gcagtaactc
    25021 attctgtaaa aaagcccaga aattgattgt aacaaaaaca gcgactctta aaagatagat
    25081 gagcatccta tctcttagag gtcgctgttt ttttattttt taagctaaat aggccattat
    25141 gcccataact cgttcaattg tttttgaata tctgcatttt ccaaaaattc atcatatgtc
    25201 gtatcagctc gatcgatcac acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt
    25261 tggataaatt gatggtcatg tgacgcaaat aagattgagc cagtaaatgc catcaagccg
    25321 tcattcaatg ctgtgattga ttctaagtct aaatggttcg ttggatcatc taatacaagt
    25381 acgttagctt tcgaaagcat catttttgaa agcatgcaac ggactttctc tccaccagat
    25441 aaaacattca ctggtttcaa tacttcttca ccagaaaaca acattcttcc taagaagctt
    25501 cttaagaagg tgttatcatc ttcttcttta cttgcatatt gacgcaacca gtctaaaatc
    25561 gttagatcag tatcgaattc tttcgttgta tcctttggta aataagaatt acttgttgtc
    25621 acaccccaac gtactgttcc agtatctggc gtaatttcac ccatgataac tttgaatagc
    25681 gttgttgttg ttatatcatt ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta
    25741 aaagatatat catctaaaat tttctttcca tcaatcgtta cacttacatt ttctacctgc
    25801 aataagtcgt ttccaatctc acgttcaggt ttgaatccaa cgaatggata acgtctagaa
    25861 gaaggttgga tatcgtctaa agtaattttt tcaagcattt ttttacgtga agttgcttgt
    25921 tttgattttg atgcattcgc actaaagcgg gcaataaatt cttggagttc tttgatttgt
    25981 tcttcttttt tggcatttgc gtttgcttga agtttagccg ctagttgact agattccaac
    26041 caaaaatcat aatttcctac ataaagtttg attttaccaa aatctagatc cgccatatgt
    26101 gtacatactt tgttcaaaaa gtgacggtca tgggatacaa cgatcacagt attttcaaaa
    26161 ttgatcaaaa attcttccag ccaattgatc gattggatat ccagaccgtt tgttggttcg
    26221 tctagcaaaa gaacatcagg tttaccaaat agtgtttgtg ctaataacac ttttaccttt
    26281 tgtccaccag ttaattcact catttgctga tggtgcaatt cttcagggat gttcagacct
    26341 tgcagtaaaa cagctgcttc tggttcagct tcccatccat taagctcagc aaattctcct
    26401 tctaattcag ctgctcgaat accatcttcg tcagtgaaat cctctttcat gtaaatcgca
    26461 tctttttctt tcagcacttc atacagacgc ttgtgtccca tgatgactgt ttcaagtaca
    26521 gtatagtctt catagtcgta atggttctgt ttcaatacgg ccatccgttc atctggtccc
    26581 atagtaacgg aacctgtgga aggctgcaga tcccctgata acactttaag gaacgttgat
    26641 ttacctgcac catttgcacc aatcaagcca tagcagtttc cgggggtaaa tttaatattc
    26701 acatcatcaa atagtttgcg gtctgaaaag tgcaaactca catcatttac agtaatcaat
    26761 ttttttcctc acttttatca ttattagttt tgacaagtct tatagaatta tataggggaa
    26821 aggtaaaaga ttcaaggcaa tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa
    26881 aataaaaata aaagcggagc gaaaatagct actataggat ttattagttt gcaacagcat
    26941 aatttttgac gttatttata agagggagta ctataaaact gtaaaagaaa tgaaagaagg
    27001 attgaaaatg gatgaaaaaa gaaccatcga ctggggctca ttgattttgg gaattttatt
    27061 tgtacttgtc tcattgttat catttcaaga ccctgtcgga aatttagttg caattgtagt
    27121 agtctttgcg atttttgcct ttattaaagg gatatttgaa ctgtttgtac ggaacagatt
    27181 aaaagagcta acaggatata aaggaaagat gcccttaata atcgggatca tcgatattct
    27241 agttggcgtc tttttcttat tcaatattgg tgcaggagtc gcagcattgc cttttgtttt
    27301 tgcagtatgg tttattgctg attcagtttt agcgttattt actgctgact tggcaagagg
    27361 agtaagtgaa ggatattatt ggtttacgat catcgtcaat attttaggaa ttttattagg
    27421 aattttctta ttgtttaatc caatttcttc tgcgttgaca ttaagctttt tagttggatt
    27481 ctattttatg ttatttggta tcacacatat cgtctatgca tttagataag taaaatcaaa
    27541 caaataaaaa agccggcttc cgattacagt tttactgttg taattggaag ccggcttttt
    27601 tatcatggac aaatatgcac gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat
    27661 gtgagaagct atatacacaa attttatctt aatactcgaa gcatagcgca gttgtcacaa
    27721 cctcttttca tataattcat tcaatactgg caaatcatgg acttcatatt tttctttgaa
    27781 tgcttggatt tcttcttcag aatattttct tggatctaaa tgcagatctt ctactggcaa
    27841 tgggcgcttg ttattgatct tcacatcgat tactacagga cgatctgatt ttgcagctgc
    27901 atcaaaggca aatcgaagtt gcgagtattc tgtgactgta aagctatttg ccccgagcgc
    27961 ttctccaact ttaccgaaat ctgcatcttc taaaaataca ccaaatttct tttgttccgt
    28021 atcttcttgt tctgcttcga tgaaaccaaa agaatcattc gaaaagacaa cattaataat
    28081 tggaagctga tatttgactt gtgtaatgat atcttgcatg accatagcaa acccgccatc
    28141 accacttaag gtaaatactt gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg
    28201 cacaccatta cccatagttg cgaaccatcc agatgtcgta tgtttttgtt taccgttcat
    28261 attcaataaa cgaatagaat ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc
    28321 gtcttcagca atacgattga tttctttata gacagcctct ggacgaatcg gttcttcctc
    28381 tcgatcttca aaagatttca accaatttac ccatttttct ttattttctt gatttgctag
    28441 gtaccagcta tcagcagggc gagcatcccc taactcgaca agtctttgca aagctgtgtt
    28501 tgcatcaccg aggatcgaca aagatgtttc atggcgtcgt ccaaattttg acgcatcgat
    28561 atcgatttgg ataaattcag cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct
    28621 accgacaaat aagatcagat cggcttctgc caatgcttca tttccgggtt tagtagcgac
    28681 acgtccagca aaaccaagaa agttttcata atcatcagga ataattcctt ttgctagaac
    28741 ggaagcgaca acaggaactg aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt
    28801 tcgcgttcct tgaccaacat aaagaactgg tttttttgct ttttcaagat aaggcaaagc
    28861 agctagcaaa tctttttctt ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga
    28921 gaaaggttgt tcgtcaattt cttcaaaacc aagatcaaca gggatagtca cgatagccac
    28981 gcctttatgc tcataggcgg ctttaatggc ttcatctact acatgcggaa gactttgtgg
    29041 tgtcatgact gtacgattgt atacgctaac atctgcgaaa ataggatttt catttagttc
    29101 ctggaaagag ttatagttca tggatgtaga agcaacttgt cctaagaacg ctactagagg
    29161 cacatggtcc atctgtgcat catacaaacc attgatcaaa tgtgtcgcac caggtcctgc
    29221 agagccgaag acagcgccga ctttccccgt taattttgca tctgcagcgg ctgcgagtgc
    29281 gccgacttct tcatgacgaa cttgaatgta gttgatttcg gttttttctt gatatagcgc
    29341 atccataatt gaattgaaag atcctccggg aatgccgtaa atatggtcga taccccagct
    29401 ttcaagaact tttaccattg cgattcctgc attgattttt gacatggtgt ttcactcctt
    29461 tagcaatgat gatacataaa ttttttgtat ctagagtata gaacttttta tcgaaaatcc
    29521 caactaattc gtcttttcac aaaaatgtag tcatgttttt gggaatatgc tagagtagta
    29581 catagataga ttcaagagag gggaatggca tggttgaatg gctattgaga cttgaacctt
    29641 ggcagcaagc acttgctggg acagctttta cttattttat gacagctctt ggtgccgggt
    29701 tggttttttt ctttaaagaa atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt
    29761 ctggtgtcat gattgctgca agtttttggt ctttgttaga tccggctatt acaaaagcag
    29821 aagaaaatgg agatattgct tggctagttg taagtattgg gtttggttta ggcggtgtgt
    29881 ttttatatat ggcagataaa acactgcccc atatgcattt tggaccacaa catgaagcag
    29941 aaggattgcc tacccattta aaacggacga tcttgcttgt tttttctatt acattgcata
    30001 acattcctga aggtttagcc gtgggggtag cattcggtgc agcagcaact gcggataatc
    30061 cgacagccgc tatattagcc gctgtttcgg tggcgctagg tattggtatc caaaacttcc
    30121 cagaaggagc agctgtatca atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct
    30181 ttgtttacgg acaagcttct ggaatcgttg aaccaattgc tggagtgatt ggagcgctac
    30241 tagtaacgaa agtggagctg ctgcttcctt atgcacttgc ttttgccgca ggagcaatga
    30301 tttatgtcgt ggtggaagaa ttgatccctg aagcacagca gacattgtcc agcaaacatc
    30361 attttgcagt ttttggcgtc atgagtggat ttatcattat gatgattttg gatgtggcat
    30421 taggataaat aaaaaaaata tatatgatat atcaaatagg gccgcgaact tatatcgcga
    30481 ccctattttt ttagtaagtt atatcataat gaagtgaatg gtatcataca ttaataatag
    30541 ttttttctat gtgtatttcc tttgatccag cgaacaagcc agatgataag tgcgattggg
    30601 attagaactt tgaccgcaaa ccataaaagc ccgcttagga cactgacaaa aagactgatg
    30661 acgaagctag ctgcaattag cactaaaaga accatgaaga tatttaagaa catatttttt
    30721 tgacgcatac aaaaccctcc tcttgttcat tctttactat agaaaaagta taacagggat
    30781 tgtttaaaaa cacctccaac tgaagactga tttttacttc atctacttaa atgatcaaac
    30841 aattgctttt tctctggttt atcatctaaa atagagcgga aaaggaggag aatatcatgc
    30901 ctaaaaatcg tcttgaagca tttacagatg ctgtaatcgc gattatcatg actattttag
    30961 ttttagaact tcatgaacct aacggagaca cgtgggaggc gctcgctgca ttaggttacc
    31021 gattttttat ctatattgtc agttttatca ttttagcgat ttattggaac aatcatcatc
    31081 atttatttca agttgtacat aagataaatg gacgggtctt atgggcgaac agtttctttg
    31141 tttttgcgct ttcgcttttt ccgtttgcaa cttcatggat cagtgagtac atcaatagcc
    31201 tggctccaga aatgacttat ggttttgtta ccttgaccgc taatattgcg tattatatct
    31261 tggcaaaaga acttgtcaaa gcaaataaag gccacgagaa agcattgaag gcgatgttga
    31321 ataattacaa gaaatcgtat ctatcgattg gtttgaatgt gttaggaatt gtcttagggt
    31381 atttagtagc gccagtagcc gtgctgatcg caaacatcgt gattctagct gcttggatca
    31441 tcccaagtcg taccatcgaa tcacaatatc ggtaaaatga tccgtgttta aaatttaaga
    31501 gaaaaatata gaaaaaataa ataaacgatc aaatatccga gtgatactag tattcaaaga
    31561 gggatgcaga tggatcaaaa aagaattcat gtaggactga ttactagttt tgtcacgatg
    31621 cttatttatt tggatcaagg tcatttgact tggacagtag ttggtgtcgt aggatttttg
    31681 acattattca cagcaataaa aaaataaaca tctttatgta atctgatgtt ctatctattt
    31741 aagaaaagca tgtttacagc agtatgattc tgttgtaaac gtgtttttat ttgtggaaaa
    31801 tttaattttt agaaaagtgt atcgaaaaat agatagttag tttttccaca gtggataact
    31861 tcgtaagaga taaaaataaa aaattagtca aagaagggcg tatagatttc ttttgatagg
    31921 agatatacaa cagaaatgaa taaatattaa cgtgttttca aacagtataa aacggtgtgg
    31981 tttttggaat aaaaaaataa catatctcat tttacacttt tgttatttta tacttaaaaa
    32041 ataacgacac agaaaatacc gtgtctcaga tttctctact acttctaaaa actactgttt
    32101 ttttcctttg aaatgccttc aaaaagtagg atcaggcaga atatactggc agaatcaagg
    32161 agaagggagc ctaaataagg agaaaaacca tagaaaatat aaagtaatta cttataaaac
    32221 aagcttttta tagaaaaata atatacctga gtagggtata aattaagacg gaattccttt
    32281 ctagattgag atattgacta ggaagaatgt taagatttta agcctttaag ctaaaaaaaa
    32341 accctttttg ttaaataagg ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc
    32401 tgcaataata tagtagatta gagatgaaaa tagatttttt taaaccaatg actgaataaa
    32461 aagaccgtca tagatacaca ttggaatgtg tctattacgg tcttttttta cgaacgattt
    32521 tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa
    32581 atgtatccca aaataatggc tagacggtct aacttatttg tcgatagatg tttgtttaga
    32641 agttgatagg cccataaacc tatcagtcct cctgttgtat tagttatcaa atcagtgatg
    32701 tccgctacgc caataccgaa taggaattga atcaattctg ccaagaagct tacaaggaaa
    32761 ataatcccta attttttaga aaaagaaagt ctcttgaaat tgacattcaa aagcacccca
    32821 aaaggaaaaa agacaatcaa gttataagtg gtctcacgta ataccgtcgt attttcaaaa
    32881 gaaaaagtag aaaaagggac gagattgaca ttgctgtaag aataagtgag aaattgtgga
    32941 agattaaatg atagcttgaa taaaatcaac cataatagaa agaataaata aatccctaat
    33001 aaaaattttg atacataccg catagacatc tcctccattt aaaaagtata ccatcgaaaa
    33061 ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca acgtagttat cgtacgaaaa
    33121 ttagaagctt ttttttgttg ggaatccggt ttctttaaca aagctaagat agcgagttgc
    33181 ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta
    33241 atttctccaa gttgtttact ttttgatact aagtttatga aaaatcaaga aatagttgtt
    33301 atactaggta atgctgacaa acgggattgg catttcatct tggttcagct ttttttccaa
    33361 ttactaggaa ggagggatag attgttcaag acttattttc gtttcaataa aatacaagat
    33421 aatccttttc gtatcatgaa tctgatgcta cttatcttta ttgtattatg gttaggttac
    33481 ataaatcatg attttttgat ttcaagtttt tcatcattag ggatctttac ttttttctat
    33541 tatcaaaaca taccgatgaa atatttgctg aaacgtatgt ttctgatcgg ttgcggactg
    33601 ttggttgctt tcgttttggg gatcttatcg acttatgttt tatggcttga accatttgcc
    33661 gtagctttgg ttgctttttc cagcagattc gtgttgcgat tattccatat ttctaaacca
    33721 ggtggcttgt tttttgcgat gctttctgcc atgggaacga gcatgcagct gccaatcgct
    33781 caattaccga tcgtctcact ttattttttt atgggtgtcg tttttgccct gattgcggca
    33841 gtgattacaa aattactgga ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa
    33901 gaaagatttc acgaagagcc tttagtgatc attgattctg ttttctacag cgctgcctta
    33961 tttctttccg tttatgtcag tcacggcttg aatctgcata atccttattg gttgaccttg
    34021 tcatgtgcat cgattttgct agcagaaaac ttggatgcga tgaagcatcg acaagtccaa
    34081 tatctgattg gatcgatggg tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt
    34141 acgcagttgc agaccatttt tctgatcact tttctatatg gaatcgcaca atttctagtt
    34201 gctagaaatt atgcagttgc taatattttt cttaatccga tggcgttgat gctatctaca
    34261 cttattcgtg gagcatactt gatttctttg atcgaatatc gttttttagg gatcgtcata
    34321 ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg ttggtttgca acattattta
    34381 acggttgttc gtcaaaaatt agaatagaag gacaaatata tgtgccaaaa gaggttatga
    34441 caagattttt gtcacaacct ctaattcaag gtgttcaaaa gccagattct cagcattaag
    34501 tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa attttatatt tattctatca
    34561 aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa tgctttcctt
    34621 gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt agtgtacaat
    34681 aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa
    34741 agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa ttctggcaga
    34801 aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa aaaacatcct
    34861 tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag caaaggcgac
    34921 tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag ctatttatct
    34981 atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc caagaactat
    35041 ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc aagtaacagg
    35101 aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct tgatctttcg
    35161 gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg atctaagatt
    35221 agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat ctcagaataa
    35281 gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc ctgattacca
    35341 ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta aatggttgaa
    35401 aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag ttgatgaaca
    35461 ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac atgattatcc
    35521 agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg agcacattgg
    35581 ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt aaaagagcgt
    35641 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    35701 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    35761 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt agtgttctat
    35821 cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta aagttaacgg
    35881 acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt ccagagatag
    35941 gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt taacttttga
    36001 taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat gactacaatc
    36061 aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca taccgccgct
    36121 tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac
    36181 ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt
    36241 tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact gctgttgatt
    36301 ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga taccattccc
    36361 taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat aaaagtatta
    36421 tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt attgatcact
    36481 ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg gaaacgtaac
    36541 tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa gcgagcaata
    36601 cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa tatctctaac
    36661 gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct ctttctattt
    36721 gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca
    36781 gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga taatagtcga
    36841 taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa attttgtgag
    36901 aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt aaagtagaat
    36961 gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc aggaataagt
    37021 ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt tatactttgt
    37081 tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg actcaaccag
    37141 tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag aaatatcagg
    37201 aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt gatggaaacc
    37261 aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca atagtaaaaa
    37321 atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta acgtatattt
    37381 atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa attgctactg
    37441 ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata tagaaattga
    37501 tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag ctattcgata
    37561 tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa gttagagaag
    37621 atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact ccgacattta
    37681 cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa agtggaatgt
    37741 ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa gggagttctt
    37801 attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt gctgttaata
    37861 ttactaactt taatgcagta taaagagtat ccagagtatt ttattttaac ttttgtatat
    37921 ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt ttctaaagtt
    37981 tgtttcttaa ttagcttatt gattc
//
LOCUS       DACCWV010000010        37505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCWV010000010 DACCWV010000000
VERSION     DACCWV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.010"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            37..2634
                     /gene="adhE"
                     /locus_tag="IVW84_000481"
                     /gene_synonym="adhC"
     CDS             37..2634
                     /gene="adhE"
                     /locus_tag="IVW84_000481"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7916708.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2863..3969
                     /locus_tag="IVW84_000482"
     CDS             2863..3969
                     /locus_tag="IVW84_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7916709.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3986..5014
                     /locus_tag="IVW84_000483"
     CDS             3986..5014
                     /locus_tag="IVW84_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7916710.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5020..5499
                     /locus_tag="IVW84_000484"
     CDS             5020..5499
                     /locus_tag="IVW84_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7916711.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5515..6810
                     /locus_tag="IVW84_000485"
     CDS             5515..6810
                     /locus_tag="IVW84_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP7916712.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6821..7645
                     /locus_tag="IVW84_000486"
     CDS             6821..7645
                     /locus_tag="IVW84_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7916713.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7810..8709
                     /locus_tag="IVW84_000487"
     CDS             7810..8709
                     /locus_tag="IVW84_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7916714.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8835..9704)
                     /locus_tag="IVW84_000488"
     CDS             complement(8835..9704)
                     /locus_tag="IVW84_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7916715.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9857..11317
                     /gene="rhaB"
                     /locus_tag="IVW84_000489"
     CDS             9857..11317
                     /gene="rhaB"
                     /locus_tag="IVW84_000489"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP7916716.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11314..12570
                     /gene="rhaA"
                     /locus_tag="IVW84_000490"
     CDS             11314..12570
                     /gene="rhaA"
                     /locus_tag="IVW84_000490"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP7916717.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12584..13429
                     /gene="rhaD"
                     /locus_tag="IVW84_000491"
     CDS             12584..13429
                     /gene="rhaD"
                     /locus_tag="IVW84_000491"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP7916718.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13431..13742
                     /gene="rhaM"
                     /locus_tag="IVW84_000492"
     CDS             13431..13742
                     /gene="rhaM"
                     /locus_tag="IVW84_000492"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP7916719.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13824..14975
                     /gene="fucO"
                     /locus_tag="IVW84_000493"
     CDS             13824..14975
                     /gene="fucO"
                     /locus_tag="IVW84_000493"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP7916720.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15102..15860
                     /locus_tag="IVW84_000494"
     CDS             15102..15860
                     /locus_tag="IVW84_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP7916721.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15960..16604
                     /locus_tag="IVW84_000495"
     CDS             15960..16604
                     /locus_tag="IVW84_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7916722.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16611..17618
                     /locus_tag="IVW84_000496"
     CDS             16611..17618
                     /locus_tag="IVW84_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7916723.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17634..18476
                     /gene="kduI"
                     /locus_tag="IVW84_000497"
     CDS             17634..18476
                     /gene="kduI"
                     /locus_tag="IVW84_000497"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7916724.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18496..19299
                     /locus_tag="IVW84_000498"
     CDS             18496..19299
                     /locus_tag="IVW84_000498"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7916725.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19548..20612)
                     /locus_tag="IVW84_000499"
     CDS             complement(19548..20612)
                     /locus_tag="IVW84_000499"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7916726.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20609..21694)
                     /locus_tag="IVW84_000500"
     CDS             complement(20609..21694)
                     /locus_tag="IVW84_000500"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7916727.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="IVW84_000501"
     CDS             complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="IVW84_000501"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7916728.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="IVW84_000502"
     CDS             complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="IVW84_000502"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7916729.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23943..24596
                     /locus_tag="IVW84_000503"
     CDS             23943..24596
                     /locus_tag="IVW84_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7916730.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24681..25586
                     /locus_tag="IVW84_000504"
     CDS             24681..25586
                     /locus_tag="IVW84_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7916731.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25588..26220)
                     /locus_tag="IVW84_000505"
     CDS             complement(25588..26220)
                     /locus_tag="IVW84_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7916732.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26540..27064
                     /gene="infC"
                     /locus_tag="IVW84_000506"
     CDS             26540..27064
                     /gene="infC"
                     /locus_tag="IVW84_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7916733.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27136..27336
                     /gene="rpmI"
                     /locus_tag="IVW84_000507"
     CDS             27136..27336
                     /gene="rpmI"
                     /locus_tag="IVW84_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7916734.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27389..27748
                     /gene="rplT"
                     /locus_tag="IVW84_000508"
     CDS             27389..27748
                     /gene="rplT"
                     /locus_tag="IVW84_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7916735.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28000..29337
                     /locus_tag="IVW84_000509"
     CDS             28000..29337
                     /locus_tag="IVW84_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7916736.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29385..31514
                     /locus_tag="IVW84_000510"
     CDS             29385..31514
                     /locus_tag="IVW84_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7916737.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31489..32178
                     /locus_tag="IVW84_000511"
     CDS             31489..32178
                     /locus_tag="IVW84_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916738.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32586..33199)
                     /locus_tag="IVW84_000512"
                     /pseudo
     CDS             complement(32586..33199)
                     /locus_tag="IVW84_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33335..33529)
                     /locus_tag="IVW84_000513"
     CDS             complement(33335..33529)
                     /locus_tag="IVW84_000513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916739.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33579..34019)
                     /locus_tag="IVW84_000514"
     CDS             complement(33579..34019)
                     /locus_tag="IVW84_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7916740.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34023..34868)
                     /locus_tag="IVW84_000515"
     CDS             complement(34023..34868)
                     /locus_tag="IVW84_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7916741.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35017..36435
                     /gene="pepV"
                     /locus_tag="IVW84_000516"
     CDS             35017..36435
                     /gene="pepV"
                     /locus_tag="IVW84_000516"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7916742.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36492..37439)
                     /locus_tag="IVW84_000517"
     CDS             complement(36492..37439)
                     /locus_tag="IVW84_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7916743.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
BASE COUNT        11827 a         6839 c         8127 g        10712 t
ORIGIN      
        1 tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat agaaaaagaa
       61 aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa agcaaatgca
      121 gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt gcatgaaatg
      181 gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga ggaaacaggc
      241 cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata tatttggcat
      301 aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa agggttgatt
      361 gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa tccgacttcc
      421 acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt ttttgctttc
      481 catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga tgctgctatc
      541 gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat cgaagcgact
      601 tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg agctggaatg
      661 gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg taacgtacca
      721 gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa
      781 acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc
      841 tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa accagatgaa
      901 ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc
      961 gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg
     1021 aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc aagagaaaaa
     1081 ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt tgatttgtgt
     1141 gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc agaaaacgaa
     1201 gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt gaatacacca
     1261 tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt gactttggga
     1321 tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat
     1381 gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc gccaaaaatc
     1441 ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga acgtgtcatg
     1501 atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg tgaagtattg
     1561 gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc gaacccatcg
     1621 acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga tacagtaatc
     1681 gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt ctatgaacac
     1741 ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa acggacatat
     1801 aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc agggactggt
     1861 tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa atatccgtta
     1921 gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt
     1981 ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat cgaatcctat
     2041 gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat caagttagtc
     2101 tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac
     2161 aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat ttgtcactca
     2221 gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa tgcgattctt
     2281 ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat gttccctaaa
     2341 tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt aggtctgaaa
     2401 ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga attaggaaaa
     2461 tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac actagataca
     2521 accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc taatccgaaa
     2581 gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg ataagcaaaa
     2641 gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaac gttgtttctc
     2701 caagttcttt ttttaatttc caagtaactg aaagttactt ttgttattta acatttaggt
     2761 caattttaga cagtttttgt tcaaaaagcg atatttatag tggacggtat tactgctata
     2821 ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa agatgattat taaagaagag
     2881 atttttgtac ctaaagaatt agtgaaagaa aaaattgacc tgctcgttga aaacctgaca
     2941 acgatcaaag acgataatgg tgaatttttg ttggactttg atggactgaa agtagatgac
     3001 aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc tatatggtat ttataaaaat
     3061 taccagatga caaaaaatcc tcgcgcttat caagtggtca atgaatggtt tgaggatcgt
     3121 atggaagaag gcgctccgcc caaaaacgtc aatacaatgg caccgctatt gactatggct
     3181 tacttgtatg aagacacgaa agattcacgc tatttgcctt atttagaaca atgggcagaa
     3241 tgggtcatga atgacatgcc tcggacaaat gaagacgggt tacagcatgc cacatatgga
     3301 ccggaaaata agaatcagtt gtgggacgat acattgatga tgacagtgct tcctttggca
     3361 aaaatcggga aactgttgaa tcgcccagaa tatttagaag aagctcgtta ccagtttatg
     3421 atccatatca aatacttgat ggataaacga acaggattgt ggtatcacgg ctggacgttt
     3481 gaaggaaatc ataactatgc cgaagcattt tgggctcgtg gaaactgctg gttgacgatt
     3541 gctattccag agatcattga aatcttagaa ttatcgaaag aagatgcatt gcgcaaatta
     3601 ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa cttatcagtc tgaaagcggt
     3661 ttatggcata cgttgttgga tgatccttct tcttacgtag aatcttctgc aactgcagga
     3721 tttgcttacg gtattttgaa agcggtccat aaacgttatt taccacaaga atataaagaa
     3781 gtcgcttatc gtgctatcca aggattgctt gaacaaatcg atgaaaaagg agaagttcaa
     3841 aacgtttcaa tcgggacagg aatgggggac agtttagatt tttatcggaa tatcggaatc
     3901 acagcaatgc cttatggaca gtcgttaacg gtgctttgtt tgacagaatt gctcgtctct
     3961 tattgttaaa agaaaggaaa cccatatgaa aaaaacatca ctgctgtttg cagcaagttc
     4021 gcttagccta ggtctgctag gtctaagcgg ctgcggaaat aattctagtg tggcaactgt
     4081 cgcagatgca gatgtaaatg agatagttac cttgcgcttt gcttatgcaa gcaacagcca
     4141 gccagtaatt gatgcaatga atgaatttgg gcgtttagta aaagaaaaaa cagatggttc
     4201 cgtacagatc cagtatttcc cagatggaca actgggtgga gaacgtgaac tgatcgaatt
     4261 gacacagtca ggagcagtag atttcacaaa agtaagtgcc tcagcgttgg aaagtttctc
     4321 taaagattat tcgatttttt cagttcctta tctattcaat gatgaagaac atttctttaa
     4381 ggtgatggat aatgaagaaa taatggagcc aatctaccaa tcgaccaaaa atttaggatt
     4441 taccggattg acctattatg attctggtca gcgcagcttt tatatggtag acggtccgat
     4501 ccatacgccg gaagatttgg agggaaagaa gatccgtgtg atgcaaagcg aaaccgctat
     4561 caaaatggta gagcttttag gcggttcgcc cgtacctatg ggaagtgatg aagtgtacac
     4621 gtctcttcaa tccaatttga tcaacggatc agaaaacaat gaatttgtct tgtatacagc
     4681 tggacatggt ggtgtggcta aatactattc ctatgatgag catacacgtg tgccagatat
     4741 catcatcatg aatgatgcca tcaaagaaca gctaactgat gagcaggaaa aagccattga
     4801 agaagcagcg aaagaatcaa cggtttttga aattgaagct tttgcaaaag caattgaaga
     4861 agaaaaagct ttagcacaag aagaacatgg agtccaattc aatgatgtag ataatacacc
     4921 gtttcgagag gcagttgccc cacttcatga tatattcaaa aacgatccta cctacaaaga
     4981 cctttatcaa aagatccaag agcaaggagc gtagctgtga tgaaaatgat tttaaacaag
     5041 gtattagaga tcctgggagc tgttttgctt gttgggatgg tcgctgttgt tttatggcaa
     5101 gtattttcca gaacggtttt aggaaatcca aatacggtca cggaagagct tgttcgattc
     5161 ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg tgggacaaaa agcacattta
     5221 gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa gtaaaatttt agaagttgtt
     5281 gttcaaagtt tatttctggt atttgctgcg atgatcatgg tgtatggcgg atggaatgct
     5341 gtcacattga caatggggca gatctcgcct tctttaagta ttcctatggg atatgtttat
     5401 ctgtcagtac ctgtatcggg ggtactcatc attatctata gcctgatcaa tctaatcgat
     5461 acattaagca acaagccagc aattcaagaa aaaatataaa aatggaggaa taaaatggca
     5521 atagaagccg gattagtgtt attttttgta tttgttgtgt tgcttgtatt agggatgccg
     5581 attgctataa gtattgcatt ctcatcgatg atgactttat tgttagttat accgtttgat
     5641 gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta tcaatagttt ttcgctgatc
     5701 gctattccgt ttttcgttct ctcgggcatt atcatgaata atggagggat agctaagaaa
     5761 ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg gggctttggc tcatacgaat
     5821 gtgttaggga atgctttatt tggttccatc tctagctcgg ccattgccgc ttctaccgcg
     5881 attggcggag tgttgatccc tcagcaagtg gaagaaggat atgaccggaa gtttgcgaca
     5941 gcggtcaata tcgcgtcagc tccgacaggt atggtcattc ctccaagtac tgcttttatc
     6001 atgtattcac ttgttgctgg tggtgcttcg atctctgctt tgtttatggg aggttatctc
     6061 gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat tcatcgttgc taaaaagaat
     6121 aaataccctg ttgtcgaaaa aggacagatg gctcatgctg gaaaaataat aaaagaggcg
     6181 attcctagcc ttctattgat tgtcatcatt atcggtggta tcttgacggg gatatttaca
     6241 gcagtagaag cttcagcgat agctgtcatt tactccctgt tgattgctat gttttactat
     6301 aaaacggtaa aactaaaaga tatacctaat atgttgtttc aatcagtagc aatgtcaggg
     6361 acgatcatgt tcttattggc tacttcttcc atgatgtctt tcgccatggc gtttaccggt
     6421 attcctcagg caattagttc gttgattatg ggcgtaacgg ataataaatt tattattctg
     6481 ttattagtca atcttgtgtt attactggtg ggtatgttta tggatgtggc gcctgctatc
     6541 ttgattttta caccaatatt tttaccaatc gtcacaagtg taggggttga tcctgtacat
     6601 tacgggttgt tttcaatcat gaatctgtgt gtaggctcca tcacgccgcc agtaggtaca
     6661 ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag aggcaatgct gaaaccgctt
     6721 cttccttttt atggggttat cttagtcgtc ttgtttttga ttacttactt cccgcaaatc
     6781 gtcatgtggc tacccaatat gctgagttag gagaatagtt atgttgaaca ttacaattag
     6841 aggacatgat ctttccgaag tccaaacgat agaagatctt gcagaaaaaa caaaagaaca
     6901 agggattcat acactgcagc tcgccttagg tctttcgttt cctgaaacac cttctggcgc
     6961 aaatgagatc aattctggga tgggaaatta tgtcaaacga gttttagaaa agcaagaagt
     7021 gaccgtagga attttaagtt gctatatcaa tatgattcat ccagatttaa cgatacgaca
     7081 agagttgctg acaaagttcg agcagtatct tcgctatgct tcttcctttg gtgcgtcgat
     7141 ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa tatacggaag aaaatttcac
     7201 agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc ttggtaaaag ctggagaaaa
     7261 ataccaaatg atggtgggga tagaaccagg tttgaatcat ccggtgtatt ctttagcaag
     7321 ggtagagcag ctgatccaag cagtagattc ggactatcta ggaatcattt tagatccgac
     7381 caatttgatc acaagtagtg attatcaaga acaagtccaa ttggtagaag aagcttttga
     7441 acgttttggt gaaaagatat gtgcggttca tttgaaagat tttcgtgtcg aacaggaaaa
     7501 aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac acaaaaatca aagaaatcat
     7561 catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa acaaaagatg atgggattcg
     7621 ttatggaaga agtcttcttg aataatgtaa agaagacgtt ggttaactaa tatttagaat
     7681 ggagggggcg gggctagaag atgccacgtc tcttcttttt tataagagta tataaaagca
     7741 gctctttttg attacaaaaa acgtggtata ataaaattgt gcataagtga acaaaaggag
     7801 gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag tcaatacgat ggtttctgat
     7861 cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa ataaaacgat ctatcatcat
     7921 catgatttct atgagatcca tgctacatta aagggagaag cgacttttta tttgaacgga
     7981 cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc attccaatga tttgcatcgt
     8041 atcgtccgcc aatcttcaga tgtattcgag cgtgtctata tctttgtcac cccttctttt
     8101 ctagaaagtc gttccacgcc atggtctaat ctatcggctt gttttcaacc aatgggagag
     8161 aagaggagtc gtgtattgca aatagaacag gaagtattga agcaaaaatt atcatttatc
     8221 gaccatcctc ctgataaaaa aatttacggt gaagacataa aatatgaaca agctctggta
     8281 gaatacttga tttttctcaa tcaagttgta caaaaagaag agagtgcgca agaacagaat
     8341 gctgtagttc ctaatgagcg tgtggaacaa atgttacaat atatctcaaa aaatttatct
     8401 catccgcttt ctttagagga aatggaaaag cagttttttg tcagcaaata ccatatcaca
     8461 cgagaattta aaaagtacac tggatttacg tttcatcagt atgtaatgaa gaaaaagctg
     8521 atctatgcca agcacttgtt gcgagaatac cgcagctcta gtgctgtata tagtaaaagt
     8581 ggtttttctt cttatccgca ttttctgaaa gcattcaaga gagagttcgg cataacgcct
     8641 aaagaatttt tgaaacgtga tacacagaaa cagttggttc attatgaaca ttttgaagat
     8701 gaacaatgag aatgatcact aaaaaataac cgcagtagct gctgttgttt acatagaagc
     8761 agaaactgcg gttattttat ttttatctat atatttcaaa atattttgaa aaaatgacgt
     8821 acatattata tgaatcagct gaatttacga aagtctctcg gcgatagtcc tgtttttgct
     8881 ttgaacaggc ggctaaagta agcaaaattc tcaatcccta tttcttcgga aatcattcga
     8941 accgtaaatt ctgtataccg aagaaggtct ttagcgtaag aaagtttttt gctgatcaaa
     9001 taatcgattg gtggagaacc gataaatttg gaaaaatctt tgctcagctg atatttattc
     9061 aataagaatt gttgttctaa ttgatcaagt gtaatcgttt ctttgaaatg ttcgtctaag
     9121 tattgcttca gtttgtaaac gtaggcggga atatcctctt cttcgaaatc tagctgatac
     9181 ttttgcaaaa tcagttcatt cagcaactcg tggatagcta cagaggactg gaaatcggta
     9241 cgggcattcg tgccttgttg catgtggatc aattctgtga tcaaatggtg gatcttgttt
     9301 tctgatggat cccctgttga atggaaggtg ttatggccgt cttttgtgaa ctcctgataa
     9361 aatgattgga cgttctgacc attcaaatgg atccaatcca tttcccatgg ttcttcgctg
     9421 atcgtttgat aacgctgata ttcttggcag tcaataaaaa ataagtctcc agcttttagc
     9481 tgttgctgat tatcattata tgtaagcgaa cctttgccag acaaagtaaa ctttatcaag
     9541 taagaaggta aatgtgctcg ttcagtatag taagggattg gtgctttgaa gtaaccgatt
     9601 tcttggatat aaaaaaataa attcctgctt ctttgtgtag gcgtcaagat cactcttttt
     9661 gagtcttccg accatgagtg gttggctttt tcccagtaat ccatgtaaac actcccaact
     9721 tgtcaagata atgcaattat ataccaaaat agtgtgaagc aaaatataag attctgtatt
     9781 atgatagcat tgttaataaa tgataacgct ttattaataa agtgctaatc aacaataaag
     9841 taaaagaggt ggagaaatga atccaacatt tgttgccatc gatatcggtg catcaagcgg
     9901 acgactaatg aaaagtacga gggatgcaaa aggttctttg atcttggaag aagtacaccg
     9961 gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc tggaagatcg attatcttat
    10021 caaagagatt ttgattggat ttgaaaaatt aaagcagaca gggattgatc gcctttttgc
    10081 ggggatcgat acatgggcag tcgattactg tctgattgat gaacaagggg atcggataaa
    10141 tgatccgatt gcttatcggg acaagcgtac caatggagca gtagaacagt tttctgaaat
    10201 ctattctcta gataagttgt atgagcagac agggattcag atccagcctt tcaatacctt
    10261 gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa gcacataaat tgttactgat
    10321 acctgattat ttagggtatg tctttactgg taacatggtg acggagaaaa ctaatgcatc
    10381 tactatgcag ctgctgaatg cagggacaaa agaatgggaa atagaacttc ttgaaaaaat
    10441 cggtgtctcg aaggaattat ttccaccatt gacagagccg ggaacgatac taggagatct
    10501 gcaaaaagaa caattttcaa aatatgattt gccagatgtg acctttatca cagtagcaag
    10561 tcatgatacg gcgtcagctg tattaggaac gccaggtcaa ggagaacgtt ggggttatat
    10621 cagcagtggg acatggtcgc ttttagggat tgaaacaaag atcgccaatg tttctcgcaa
    10681 tgctttttct gaaaattata cgaatgaatg gggtgcccat aacacggtgc gttttttgaa
    10741 aaacatcatg gggatgtggt tgatccagga agttgcccga atgcaagagt acgagtacag
    10801 ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg ctgcagcaag tgatggaaat
    10861 caatgaccct cgatttttga atcccccaaa tatgatagaa gagattcagc aatactgttt
    10921 tgaaacagag cagccagtcc ctgtctcatc gggagctatc gctcgttgtg tgtatgataa
    10981 tcttgctctt tgttacgcac aagagctgat caaagtagaa cacttaactg gaacgaaatt
    11041 ggaaaagctg cacatcgtag ggggcggatc gaataatcgt ctactgaatc aattgacagc
    11101 agattatgcc ggaatcattg tagaagcagg acctagtgaa gccacagcaa tcgggaatgt
    11161 tgccatgcaa atgctcagta caggtcagtt ttcgaatata gaacaagcaa gaaatgtaat
    11221 caaacagtcg tttccatgtg aaacttttta cccagatgaa aataagcaaa acacgatgga
    11281 atggtacgtg aataaaatca aaggagtaat cgtatgaaca cgattgaaag aaattacgaa
    11341 caagcaaaag aaaaatatgc aactatcggt gtagatacag atgctgtact agaaaagata
    11401 caggacatca aaatttctat gcattgctgg caaggagatg atgtcaaagg tttcttgaca
    11461 ccggatggcg aattgaccgg tggaatcatg gcgacaggga attttccagg agccgcacga
    11521 acacctgaag agcttagaca agatctggag aaggcttatt cgttgatacc gggcaaacac
    11581 aaattgaacc ttcatgctat ctatttggat acagaagaat ctgtcgatct aaatgaaatt
    11641 gagccgaaac attttgaaaa atgggtagag tgggcaaaaa aagaagaaat cggtcttgac
    11701 ttcaatccga ctttcttttc gcatccaatg atgaaagacg gctttacatt ggctcatcca
    11761 gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa gaagccgcaa aatcgcggag
    11821 tactttggga aagaaactgg gcaaacaagt atcaataact tttgggtacc tgatgggatg
    11881 aaagataatc cgattgatcg ttttacccca cgggaacgtt taatggaatc actcgatgaa
    11941 atttttacag aagagttaga tgaacggtat gtacaagatg cagtagaaag caaactattt
    12001 ggtttaggcg ctgaagctta tacagtcggt tcccatgaat tttatatggg ttacgggttg
    12061 actcgtgaca aacttgtttg tctagatgct ggacattttc atccaacaga agtgatttcc
    12121 aataaattat cttcattagc tttattcagc aaaggaatca tgttgcatgt ttcccgtcca
    12181 gtgcgttggg attcagacca tgtggttttg atggatgatg aattgcagga aatcgctaaa
    12241 gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg gtctggattt ctttgatgca
    12301 acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa atacacaaaa agcactgctg
    12361 aaagctatgc tggaaccagt tgagcgattg aaagaaatgg agctggcttt tgactttact
    12421 tcaagaatgg cttatacgga agaattgaaa gatttcccgt atgccgatgt atggaattat
    12481 ttctgttaca aaaatcaagt tccagttggt ttggattggc tggaagaagt ccaggaatat
    12541 caaaaagacg tgttggaatt aagaaaatag gagtgtggct atattgaaaa aaatagatat
    12601 tttacaagcg ccttatgtga aagaaatgat cgaaacgacg tcgaatcttt atcgcttggg
    12661 atgggatgaa agaaatggcg ggaatatttc ttatttacta aaagaagaag agatcacacc
    12721 gttcttggat cctgaaaaag tgatccgccg catctcgatg atttttgatg ccagccgttt
    12781 agcaggatgc tattttattg taactggttc aggaagatat tttaaaaatg tagcgagcga
    12841 acctgccgag aatcttggaa tcgttcgagt tgctgaagat ggcaaaacac tggaactttt
    12901 gtgggggctg gaaaacgaat gccttcctac aagcgagttg ccaagtcatt tcatgagtca
    12961 tatcgcccgt ttagaagtag acccagataa tcggatcgtt atgcattgcc atgctagtca
    13021 tctattagcg atgagtttta cgcatgaatt agacgaacgt tcctttagtc gtacgctatg
    13081 gcaaatgtgt acggaatgct tagttgtttt tccagaagga atttccatca ttccgtggat
    13141 ggttccggga acaaatgaga ttggggaagc aacagctgaa aaaatgaaag aaactcgatt
    13201 ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa gacatggatg aagtctttgg
    13261 tttgatcgag acagcagaaa aggcagcaga agtctatacg tacgtcaaag cgcaaggacc
    13321 aatcttgcaa acaatcacgg acgaaaacct gtggcattta gcagatgcgt ttggtgtcac
    13381 tccaaaagca ggttatttgg aggaagttca tacgaaagca ggtgtctgac atggtgaaaa
    13441 aagctttttg catgaaagtt tacccagaaa aacaagaaga atacaaaaaa agacatgatg
    13501 aactttggcc agaaatgcgt acagcacttc gtgaacacgg ggtcatcgac tattcgatct
    13561 atcttgatcc gagtacaagt ttgttatttg gttacttagt gatcgaagat gaaaagttat
    13621 gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg gaagtacatg gaagatatca
    13681 tggagacaaa tccagatgcc tctccagtca gttgggaatt gatgccagtc tttgaactgt
    13741 agcaacaagc aactgggcaa ccggttgctt ggggttcttt cgtttttatt gtgaaaaaaa
    13801 atacaaatta ggaggaatag aaaatgagta atcgaatgat tttaaatgaa acatcgtatt
    13861 ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca gcagcgagga ttcggcaaag
    13921 cagctgtcat tactgacaaa ggattgatcg agcacggtat agcaacaaaa gtaacagaac
    13981 ttctagataa agcagcaatc gcttatgctt tatacgacgg gatcgtacct aatccaacga
    14041 tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc agaggcagac tgtttgatcg
    14101 caatcggtgg aggttcgcca atcgatacgg caaaagccat tgggattatt ttgacgaatc
    14161 cagagttttc agacgtcatt agtttagaag gtgttgctga tacgaaaaat ccatgtttac
    14221 ctatattagc tataccaacg acttctggaa cagcagcaga agtaacgatc aactacgtca
    14281 tcacagatga agtaaatcac cgaaaatttg tttgtgttga tcctcatgat atcccaatcg
    14341 tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa aaaattggct gcatcaacag
    14401 gtatggatgc tatgacgcat gctattgaag ggtttatcac aaaaggcgct tgggaaatga
    14461 ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca ttctttggaa gtttctgtag
    14521 atggcgatca aaatgggcga gaaaaaatgg cattaggaca gtatattgcg ggaatgggct
    14581 tctcaaatgt cggtttagga ctggtacacg ggatggctca cccgttaagt gcatggtaca
    14641 acattcctca tggcgtagcg tgtgcagcac ttttgccaac tgtcatgaaa tataacaaag
    14701 aatatactgg agaaaagtac cgtgagatcg ctcttgttct agggattaaa ggagcagctg
    14761 aaatgtcttt agaagatgta agagaagcgg cttgtggtga gatcgatcgg ttgagcaaag
    14821 cggttggaat cccagaaacc atttctgaat taggtgtaaa agaagcagat attccagcaa
    14881 ttgcagaaga tgctttgcga gatgtatgca caccaggaaa tccaagagaa acgacagtag
    14941 aagaaatcat tgctttatat caaagtttga tgtaaaaacg aagcggatct tcaacattaa
    15001 attgcctgta aagagaaatg gtagtttata tctggatatc tcttttaaat tgatggtatt
    15061 tccgctataa tttttcttat aaagaaatag gagaatatta tatggaaaat aaaaagcctt
    15121 atggaactgt attgatcaaa gcctctaaaa tactagacta tttagcggaa aaccaagatg
    15181 tctctttgca gacgatcgca aagggtgtgg gaatgacctc ttctacagtc ttgaaaatac
    15241 tagatacttt gttgatgatc ggctacgtaa ataaaaattc agagaaaaac tatcgtttag
    15301 gtgcaaagct tattcgctac gcgaataaaa acattgaaca aatcgatttg gtagagttga
    15361 ctttaccgtt tttagaacag ctgcagcaaa agatcgatga gactatccac ttaggggtat
    15421 tggacaacaa tgagatcttg tacgtgaaca aactagatcc aaaacatcaa acgatccgca
    15481 tgtcttcaaa aatcggaatc acccgaccat tatacaattc tgcgatgggc aaagccgtat
    15541 tagcagaatt cagtgaagag cagtacactg attatcttga aaagcatccg ttgatccctt
    15601 acacagaata tacgatcacc aatgcattac ggttgaaaaa ggaaatccaa acggtaaaag
    15661 aaacccacat cgcatttgac gatgaagaaa tcgaacgtga tattttttgt ataggagcct
    15721 caatcgtgaa aaacgaacaa atcattggtg catttagtgt aagtatgccg aaatatcgtt
    15781 taacagatga gacaaaagaa caaataatca gtgccctcaa gcaaacaaaa agcgaaatcg
    15841 agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt tttcttttct gccgtataat
    15901 atgggtatag atttccaagt tataaaataa aatttctaaa taggaaatag gtggagaaga
    15961 tgaaaaaaat tgatatttta tctcgattga aaaacgccgg agtgattgcg gtggtacgcg
    16021 gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat catcaaaggt ggtctaactg
    16081 gtattgagtt gacttttaca gtccctcaag cagatcaagt aatcaaagaa cttttgtcat
    16141 tctacaaaga ccaaccagaa atagtgattg gcgcaggcac agtattagac gctgtcacag
    16201 ctcgcttggc tatcttagct ggcgcagagt atattgttag cccttccttt gatcaagaaa
    16261 cggcagagat gtgcaatctg tatcaaattc cttacttacc aggatgtatg actattaccg
    16321 agatcaaaac agcgctgaaa agcggggtag atatcgtcaa actatttcct ggaagcgctt
    16381 atggaccaag tatcatttct gccttcaagg cgccgatgcc tcaagtcaat atcatgccaa
    16441 ctggcggtgt gagtctggac aatatgaaag agtggttcga tgctggtgtc gtaacagtcg
    16501 gagtaggtgg gaacctttta gcacccgcag ctacaggaga ttttgacaaa gtaacggaag
    16561 tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag gtagctgctt atgggaaaag
    16621 tagtgacact cggagaaatc atgttgcgat tgtccacaga gcagggaacc cgtatcgatt
    16681 caagtgaacg attcgctgcc cattatggcg gaggggaagc aaacgtagca atttctttag
    16741 ctaattacgg acatgaagcg atttttgcta caaaagtacc tgataacgca ttgggaaaag
    16801 cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa acaggtatta tctggtggat
    16861 caagactagg tacttattat atggaaactg gtagtggcga acgcgccgcc tcagtgatct
    16921 atgatcgtgc cggatcaagt tttgctgaaa tggatcattt ggaatggtcg ctagaggagt
    16981 tattccaagg aatagatata tttcatgttt caggaatcac cccagctttg tctaaaaaat
    17041 ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa agctggctgc ctgatcagct
    17101 tcgatatcaa ctatcgtgga aaattatgga cacagcaaga agccagcgaa gtaatccatc
    17161 agcttttacc tttagtagat atctgctcag ctggaaaaat ggatgctctt tatttgttag
    17221 gtatcgagaa agcgccagaa gcagaagaac aacctctaat ttattattat caaaaaatgc
    17281 aggaaaagtt cccgaatatt caggtgtttt attcgaccaa gcgtaccgtt cattcagcaa
    17341 gtgaaaatga attgaagggg acattgtgga tgaaccatga atatgtggaa tcccaagtcc
    17401 atagaatcac cccaatcgtg gatcgagtag gtggcggaga tgcttttgca ggtggcgtgc
    17461 tccatgggat tttatccaat atgtctccgc aagaaatcat tgattttgct acggcagctt
    17521 ctgccttgaa gcatacgatc catggggact gcaaccaatt tagtcaagaa gaagtcgcac
    17581 aatttttagc atgcggctct ggaaaaatca ttcgatagaa ggagtagaag aaaatgcaag
    17641 aaatggaaac acgttataca cacagtccag aagatatccg tcattattca acggaacaat
    17701 tgagaaaaga atttttagta gaaaaggtat ttgaaccagg gaaaatcagt ttgacttata
    17761 cgcacaacga tcgaatgatc tttggtggag tgacacctac tgaaaaagaa ttagaaatca
    17821 cattagacaa agaactaggt gttgattact tcttagagcg tcgtgaacta ggtgtgatca
    17881 atatcggtgg accaggattc atcgagatcg atgggcaaaa agaagaaatg aaaaaacaag
    17941 acggatacta tatcggaaaa gaaacacgac atgttgtatt ttcttctcaa gatgctgaaa
    18001 acccagctaa attctatatt agttctgttc cagctcatca taaatatcca aatgtaaaaa
    18061 tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg cctgacactg aatgaacgta
    18121 aaatctacca atacatccat ccaaatgtat gcgaaagctg tcaattacaa atgggttata
    18181 cgattttaga accaggaagc gcttggaata cgatgccttg tcatacacat gaacgtcgta
    18241 tggaagctta tgtctacttt gattatgcca atgaagatac acgtgtgttc cacatgatgg
    18301 gcaaaccaga tgaaacaaaa catttagttg tagacaatga acaagccatt atttcgccaa
    18361 gctggtcgat ccattcaggt gtaggtacaa gcaattactc attcatctgg gcaatgtgcg
    18421 gagaaaatat cacctataca gacatggata tggtgcaaat ggatcaatta aaataagcat
    18481 aagggagaga aaatgatgga atttagtatg gatatgtttc gtttagatgg aaaagtagca
    18541 ttagtcaccg gtgctgtcta tggtattgga tttgagattg cacgctcttt agctacagca
    18601 ggagcaaaaa tcgtatttaa taacttgaat caagaatcag ttgatgaagg tatagcgcat
    18661 tataaagaag caggcatcga tgcaaaaggt tatgtctgcg atgtgaccga tgaagaagcg
    18721 gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt cagttgatat tctagtcaac
    18781 aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg atgcggcaga cttccgccaa
    18841 gtaatcgacg tggatttaaa tgccccattc atcgtttcaa aagcagttat tccggatatg
    18901 atcgaaaaag gcggcggtaa aatcatcaat atctgctcca tgatgagtga attaggacgc
    18961 gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa aaatgctgac gaaaaacatc
    19021 gcttctgaat acggtcaata caatatccaa tgtaatggaa ttggaccagg ttatattgct
    19081 acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg gtgaacgtca tccattcgat
    19141 caatttatcg ttggacgtac gccagcagca cgctggggcg agccaatcga tttggctgga
    19201 ccatctgtct tcttggcttc tagcgcatct gatttcgtta acggccatgt cttgtatgtg
    19261 gacggaggca tacttgccta tatcggaaaa caaccttaaa agagctttag tacactgttt
    19321 agtggagagg ttgtgaaatc agcggattga acgaataccg aaggacagct gattaaacat
    19381 catttattcg aaaaaagagg ctgggacaga agcatttaac tccaagaaat aagaaggaat
    19441 tcacgaaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttt
    19501 tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
    19561 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
    19621 tccttcactg gtccggaaaa gatcagagat tgttggtgta gggccgcggt attcgttgcg
    19681 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
    19741 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cacggcattt
    19801 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
    19861 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
    19921 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
    19981 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
    20041 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
    20101 gtttgagttt cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
    20161 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
    20221 gcttctttgg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
    20281 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggacttt caaggcagcg
    20341 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
    20401 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
    20461 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
    20521 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
    20581 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
    20641 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaagaatgc
    20701 cagatttctg gtcaacaatt acgattccgc aatcgccccc acctgcacca gaagattttg
    20761 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
    20821 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
    20881 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
    20941 tattttttaa gaaaagctga taagccacca ttttatcttc tctcgaacgg tgaacttgat
    21001 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
    21061 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
    21121 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
    21181 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
    21241 tggctagtgc tgcaatcttg aaaatctcca actgagataa attcaacgcg taaaaaacat
    21301 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
    21361 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
    21421 tttcttgtgc atacttttca gtcaagcgaa tagcagcaag gatataatga aaaggatttt
    21481 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
    21541 attgtgcaga ttggatactt ccgacttttc gtgcagattc tacagtgact gtcacgaatt
    21601 gatcgactgc agcgataact gctggatggc ctgtttcaac aactgcatat tctccggcaa
    21661 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
    21721 caattcctgg accagcatgt gcagtaatta cttgttcttt gctaaataat ttggtaaatg
    21781 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
    21841 ccatcgtaaa gtagcatgga atgccttgct ttctcaattg tcgcaccgca tccatcgctt
    21901 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
    21961 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
    22021 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
    22081 atgtttcgac tgtccgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
    22141 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
    22201 aagaacttaa gtcgtcatgg cctttttccc attcgacgaa accaccgaaa atacttcggc
    22261 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcatct aatccaagct
    22321 taagagcagt attgcaagcg cctgctaaag cagctagccc gctggcagag gaggctaatc
    22381 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
    22441 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgcaagg
    22501 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
    22561 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
    22621 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
    22681 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
    22741 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
    22801 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
    22861 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgattgtga ttcatccatt
    22921 gtctgtgcca acgtttctgg tgaattttca atgattgctt gttttgcttg cttcgtcaag
    22981 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
    23041 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
    23101 gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt ttgtaaaata aatcggctgc
    23161 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
    23221 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
    23281 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
    23341 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
    23401 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
    23461 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
    23521 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
    23581 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
    23641 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
    23701 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
    23761 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
    23821 actttttttc ttctttgact agaaaaaaat acaattgaca ctactagata ttgggggtat
    23881 atttaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
    23941 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
    24001 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
    24061 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
    24121 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
    24181 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
    24241 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
    24301 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttggagcgta
    24361 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga tccaagtcta tacgattatt
    24421 acaaagaatt gaacagacgt tacgatcaat ggtatgaaga atacaaagaa agtccaaaaa
    24481 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
    24541 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtagcc atttgattta
    24601 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
    24661 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
    24721 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
    24781 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
    24841 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
    24901 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
    24961 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cgaatgccag
    25021 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
    25081 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
    25141 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
    25201 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcattggct atttatcgca
    25261 agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct gatcgaagca cgacaagcag
    25321 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
    25381 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
    25441 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
    25501 ccgttttcgg atggggaatc gccttgatca tcagtttggt cttatctatt tggatgctta
    25561 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
    25621 gatcacaagt gcttgctctt ggttgacgat tagtcccgcc aaagaatctt gagataaagc
    25681 gattgctgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
    25741 cgaaccggtc aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
    25801 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
    25861 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
    25921 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
    25981 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaaac
    26041 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
    26101 gagatagctt tggtcacccc cgttgatcag aagaccagag aagatctcct catcttctaa
    26161 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
    26221 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagtc
    26281 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
    26341 attagttttt gctatactat gatggttgag aaataaaagc agaagcaccc gcttctcgcc
    26401 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
    26461 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
    26521 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
    26581 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
    26641 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
    26701 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
    26761 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
    26821 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
    26881 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
    26941 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
    27001 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
    27061 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
    27121 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
    27181 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
    27241 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
    27301 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
    27361 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
    27421 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
    27481 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
    27541 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
    27601 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
    27661 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
    27721 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
    27781 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
    27841 ataacgtata agtctaaaat agcataatta aggtttttta attgaaaact gaacatttat
    27901 ggtcaccctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
    27961 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
    28021 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
    28081 atggtcggct ttgccatcat ggctactgta tggatgggat tagcgctagt cggaaacacg
    28141 ttttcaccaa acccggcgat ggaagggcaa ggagtaattg atgtgttgac gcatatctat
    28201 acgacggggc cagctgagta cgccaaatcg atcttggtaa atgtcttctt tggtgccttt
    28261 ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc tgattcgaaa ggtagttgaa
    28321 cttggagggg acaagccaag aatcacgatg agcttactat gtattgtaac cgctgttata
    28381 ttcatgtcga tgacaggaat cggaccagtg atttctattg cagttattgt gttgccaatc
    28441 ttgatgtcat taggtatttc agtaccggtt gcactgttct catttatggg ctcgattatg
    28501 gctggaatct ttgctaatat cgtcaatttc aaacaatatc aaacgattta tgctggtttc
    28561 aatccagcgg ctgaaagcta tacttataat gactatttcc aaattggtat gattgggatg
    28621 attgtaagct tagttgtggt actaacggtc gctaatatat caatgaacaa aaagaaacgc
    28681 tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg caccaatgat ctcatggtta
    28741 gccgtattat taccagtttt aggggtcgtt cttttagatc ttccaatcat cttaggtttt
    28801 attctagcag gtatctgggc attgctgttt actggaaaat tgcgcggagg ttataaagaa
    28861 atctgtcgtc agtttgcgaa attatttaca gatggcgcag tagatgttgc accaatggtt
    28921 ggtttcttga tgaccttagc gatgttcaat aattcagctg cgtatgcttc tccttatttt
    28981 tcagctattt ttggagattg gataccgcaa tcaccattag tcttggcaat cgtatttgct
    29041 atattgacac cactaggatt tttccgcggt ccaatgaatc tagtcggttc aggctcagct
    29101 attttagctg tcgtattagc tgttaatcca acgatgtcgc cagccttttt gttcccgtta
    29161 tttgcaatca caacgattgc tcctcagcat ttagatatta cacaatcttg ggttgcttgg
    29221 ggattaggtt atacaaaagt aacttctcga gaatatatga agaaatccat cccaactggt
    29281 tggatcatcg gagcgatctt atgcctgatc acgttcttat tgtacgggaa cgcctagtaa
    29341 acaaaacgtc gataaaaacg attagatgaa tggaggaaaa gaaaatgaaa cgttttgtgc
    29401 gaatgggaat tgatgttggc ggtacacata cgaaagcagt cgcaatcgat aatgcgacgc
    29461 acgaaatcat cggaaaatct tcagtgaaaa caacccacga tgatgtccgt ggagtagccg
    29521 caggtgtcgt tcagtctttt caaaattgtt tgcgggaaaa taatatcagt ccagaagatg
    29581 ttgtgtttgt tgcacattct accacacaag cgactaatgc actgattgaa ggagacgtag
    29641 caaaagtcgg cgtaatcggg atggcaaaag gtggattaga aggtttttta gcaaaacgac
    29701 aaactagatt gaacgatatc gatttaggga ataagaaaaa aatagagatt gtcaatgcat
    29761 tcttacctgt caaacactta aatgtagacc gtgtttcaga aacgatttct tctttagaaa
    29821 gagaaagagc ggaggtcttg gtttcttcta tggcatttgg agtagataat ggggaaccag
    29881 aacgagttgt ttatgaagca gcatcggtaa aatcgatccc tacaacgatg gcttctgaca
    29941 tcacaaaatt atatggattg acaagaagaa ctcggacagc agcaatcaat gcatctattt
    30001 taccaaaaat gctagatacg gcaacctcta cagaagattc cgtacgagaa gcaggggtca
    30061 atgtttcttt gatgattatg cgcggagacg gcggagtaat ggaaatcaat gaaatgaaaa
    30121 aacgacctgt tttaaccatg ctttccggtc cagcagcatc agtcatgggc tctttgatgt
    30181 acttgagagc gtctaatggt gtttattttg aagtaggcgg aacaacaacc aatatcgggg
    30241 tcattaaaaa tggacgtcca gcaatcgatt attcaattgt aggtggccac ccaacatata
    30301 tctcttcatt agatgttcgg gttctaggtg tggcaggcgg ttcgatggta cgggctaatc
    30361 agtcaggaat catcgatgtc ggtccacgtt ctgcacatat tgccggtcta gattatgcag
    30421 tatttacgga gacagaaaaa atcaaaggtc ctaaggttga atttttctct cctaaagaag
    30481 gagaccctgc tgattacgtc aaggtagtga tggaagacgg agaagaagtg actattacaa
    30541 atacatgtgc agccaatgtc cttggattag tgcaagaaga acatttttct tatggaaatg
    30601 tcccatctgc acgaaaagcc atccaagcat tagctgatta ttgtcatacg acggttgaag
    30661 acatcgcaga acagatcatg gaaaaatcct atgctaagat tgaaccggtg attttagaat
    30721 tggcagacaa gtatcacttg gagaaagatc agatttctct tgtaggtgta gggggcggcg
    30781 ctgcctcatt gattacttac tttagtaata aaatgggtgt gaaatattcg attcctgaaa
    30841 acgccgaagt gatttcttct attggtgtag cacttgcaat ggtccgagat gttgtagaac
    30901 gtatcatccc ttcgccaagt aaagaagata tacgatcgtt gaaaaatgaa gcgatgaata
    30961 aagcgattga atcaggcgct acaccagaat cgatcgaagt ccacgtagaa atcgatccac
    31021 agacttcaaa agtaacggct atcgctactg gttctacgga agtgaaagca accgatctaa
    31081 ccaaggagat cacaacagaa gaagcactag agcttgctgc agaggatatg cgattaaata
    31141 aaaatgaagt ttgcttattg gaaaatacac ctttctttta tgtatgcggc gaacagaatc
    31201 gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa aggatttatc aaggtccaac
    31261 gtggacatgc ttcatgtatg aaaacaactg cagccaatta tatgacagct gtcgaacaac
    31321 tttgggaaga tatggcagtt tatcaaactg aattgattgc ccgaccagaa ttttatctct
    31381 gcttaggagc aagagtctct gattttacag cgacggatct ggaacaacta cagcttttga
    31441 tggatctgga agtatctact atggaaccgg aggaagaagt gatcgtcgtt gccggaaata
    31501 tcaaacaaac ataagttaga agagttacta aacgaattac aacagatccc cgaagaaatt
    31561 tggggattct accagtttcg gcgagattta ttttggaaaa agattccttt gtccaagcag
    31621 aaaattctta tacaacaatc aatcgattgt ggtatcgaaa cggcatgtag catcaaaaaa
    31681 aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa tggctgttcc aatcgtttcc
    31741 tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa cttatgcaga agatgaaggg
    31801 attcgattga tgacggagcc gttagaaaaa ttaaaatgtt caggactagc ttcgatctct
    31861 aaagaaacag cgcaagcttt gattattgga catgaactat ttcaccacat cgaagcttct
    31921 gttaagggaa tttatacgca aagcgaaaaa attgtgctgt ggcggttgcc tttttataca
    31981 catcaatcaa acattcgtgc attgagcgag atagcagcga tgagtttttc aaaagagatg
    32041 aatcaaagcg atttttcgcc ttatgtatta gaggcagtct tactttggcc atataatgaa
    32101 attcgtagcc aagggatctt agaagaaata aaaaaaatag aaaaaagatg tacggagtat
    32161 gatttagatt acaaataaag agactgtgac aaaagtcgtg tcacagtcct ttttttccga
    32221 ataaacgatg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa caaacatgag
    32281 aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct
    32341 tggaaaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat
    32401 aagaagctat ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
    32461 tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta gctcaaaaaa aacggcaaaa
    32521 tatgaggaac tattttgccg tttctttctc taatactcag gtcaaaacgc tgattccaaa
    32581 acctcttatt tgatgggtct gtcctgaata tcttcaaaat aatcggggta attatgaatc
    32641 acgtattctt gctcctcgat aatcatatgt aaatgcatcg tgcaaagaaa tccgacttca
    32701 tcaaagttat gatcaatcat tgattgaagc attgtctgat gttcatctaa tactcgaccc
    32761 cagtctaatt ctgaaatcgt caaacgtaac catctgaaac ggttcaagtg cgtactataa
    32821 ctttctagcc aatcccaaat ttctcgtttt ccagcaattt cataacaagt tcggtgaaaa
    32881 tcagtatcca aatcaaaaaa agctttttta ttttgagcga ttactgcctg atcggttttt
    32941 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
    33001 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
    33061 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
    33121 ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
    33181 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
    33241 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
    33301 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttgtatcg
    33361 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
    33421 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
    33481 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
    33541 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
    33601 gaagcgtacg cttggaataa ctgatctaac tccggattga ctttgctcag cttcactaat
    33661 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
    33721 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg tccccgtata cttttggata
    33781 gttggatgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
    33841 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
    33901 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
    33961 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
    34021 actcatgcct cgttttccat tgatttttta caaaaataat tcatccattt cggaagtgct
    34081 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
    34141 ctatgaagat aaatagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
    34201 cctgtgatca tcccagctaa agtgtcaccc atgccgcccg ttgccatagc agcagaacct
    34261 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
    34321 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
    34381 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
    34441 tagggtagca aaagatgatt agcagcaagt aaacttaaag cagagccatc aatcaccagc
    34501 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
    34561 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
    34621 tcagtatccg tccaaactac aatcattgct tctggcagat gactgtgaag agcactccga
    34681 tttttttcat cagttactac agtcgtgaga cccgctccgc tgtgtacggc agctaaagca
    34741 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
    34801 gttcctttat gtgaaatttt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
    34861 atcagcatct aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
    34921 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
    34981 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
    35041 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
    35101 gagcgcgatg actcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
    35161 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
    35221 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
    35281 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
    35341 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
    35401 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
    35461 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
    35521 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
    35581 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
    35641 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
    35701 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
    35761 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
    35821 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
    35881 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
    35941 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
    36001 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
    36061 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
    36121 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
    36181 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
    36241 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
    36301 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
    36361 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
    36421 cgtttggcga aataattaat taaaaagagg atcaagaatt gcaaattatt tttgcagtct
    36481 tgatcctttt tttactttct actgtggtat tcatgataaa catgatatag attcaattca
    36541 tattctttct tagcgttcgt gacgactgta tccaaaatca accctgttac taatgaaaga
    36601 agtgctaaag tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt aataaagctt
    36661 gtcagcatat attcgcgaat cacaggaatc cccgtgacca atccaaataa aaagaagaag
    36721 atggtccaaa tcccaaagaa aagcaaaggc ttataatctt taaacatttt aataatcatc
    36781 atgatcactt tgaatccgtc agagaatgta ttcaattttg attcgctgcc ttcaggacga
    36841 tcacgataat cgattgggat ttcaacgaac ttgaattttt tatctaaagc atggatcgtc
    36901 aattccgttt cgatttgaaa accagaactc ataataggaa aacttttgac aaagatacga
    36961 ttgaatcctc gatagccggt catgacatca agaatttttg ttttatacat tctagtaatt
    37021 gtatttctaa caaggttatt tccaaaatcg tggaaaggtc tcttattctc atcaaagtag
    37081 gttccatttg ataggcgatc accaatgacc atatctgctt caccagaacg taatttctcg
    37141 agcaaaccat gtacagcttc tgcaggataa gtatcatcgc catctaccat taaataatag
    37201 tcggcatcga tatcaaagaa catttggcgg atcacatttc cttttccttg acgaggttct
    37261 ttttttacga ttgctccacc ttgaacggcc aattcgtatg ttcgatcggt cgaattatta
    37321 tcatagacat aaatatctgc ttctggtaat tctcgtttaa agtctgcaat gactgttgaa
    37381 attgttgctt cctcattgta acaaggaatc agaacagcaa ttttttcttg ttgttccatt
    37441 tttcactctc cttaactata gaaaacaaaa gctaaattaa aaatacctca taatcaaagg
    37501 aaatg
//
LOCUS       DACCWV010000011        37244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCWV010000011 DACCWV010000000
VERSION     DACCWV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.011"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..839
                     /locus_tag="IVW84_000518"
     CDS             <1..839
                     /locus_tag="IVW84_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7916744.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="IVW84_000519"
     CDS             complement(1263..1433)
                     /locus_tag="IVW84_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7916745.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="IVW84_000520"
     CDS             complement(1627..1806)
                     /locus_tag="IVW84_000520"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916746.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1816..2040)
                     /locus_tag="IVW84_000521"
     CDS             complement(1816..2040)
                     /locus_tag="IVW84_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916747.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2193..3305
                     /locus_tag="IVW84_000522"
     CDS             2193..3305
                     /locus_tag="IVW84_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7916748.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="IVW84_000523"
     CDS             complement(3383..3676)
                     /locus_tag="IVW84_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7916749.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="IVW84_000524"
     CDS             3844..4158
                     /locus_tag="IVW84_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7916750.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="IVW84_000525"
     CDS             4179..6158
                     /locus_tag="IVW84_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7916751.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="IVW84_000526"
     CDS             6171..6497
                     /locus_tag="IVW84_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7916752.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="IVW84_000527"
     CDS             6513..7031
                     /locus_tag="IVW84_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916753.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="IVW84_000528"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="IVW84_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7916754.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="IVW84_000529"
     CDS             complement(8467..9972)
                     /locus_tag="IVW84_000529"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7916755.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="IVW84_000530"
     CDS             complement(10064..10654)
                     /locus_tag="IVW84_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7916756.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="IVW84_000531"
     CDS             10833..12479
                     /locus_tag="IVW84_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7916757.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="IVW84_000532"
     CDS             12528..13247
                     /locus_tag="IVW84_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7916758.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="IVW84_000533"
     CDS             13726..14673
                     /locus_tag="IVW84_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916759.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="IVW84_000534"
     CDS             14685..15605
                     /locus_tag="IVW84_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7916760.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="IVW84_000535"
     CDS             15646..17103
                     /locus_tag="IVW84_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7916761.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="IVW84_000536"
     CDS             17448..18062
                     /locus_tag="IVW84_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7916762.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="IVW84_000537"
     CDS             18055..19779
                     /locus_tag="IVW84_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7916763.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="IVW84_000538"
     CDS             19776..21227
                     /locus_tag="IVW84_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7916764.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21699..23852)
                     /locus_tag="IVW84_000539"
     CDS             complement(21699..23852)
                     /locus_tag="IVW84_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7916765.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23854..24900)
                     /locus_tag="IVW84_000540"
     CDS             complement(23854..24900)
                     /locus_tag="IVW84_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7916766.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25050..26339
                     /locus_tag="IVW84_000541"
     CDS             25050..26339
                     /locus_tag="IVW84_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7916767.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26352..29054
                     /locus_tag="IVW84_000542"
     CDS             26352..29054
                     /locus_tag="IVW84_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7916768.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29039..29896
                     /locus_tag="IVW84_000543"
     CDS             29039..29896
                     /locus_tag="IVW84_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7916769.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29967..30824)
                     /locus_tag="IVW84_000544"
     CDS             complement(29967..30824)
                     /locus_tag="IVW84_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7916770.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31035..33206
                     /locus_tag="IVW84_000545"
     CDS             31035..33206
                     /locus_tag="IVW84_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7916771.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33274..33615
                     /locus_tag="IVW84_000546"
     CDS             33274..33615
                     /locus_tag="IVW84_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7916772.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33891..35645)
                     /locus_tag="IVW84_000547"
     CDS             complement(33891..35645)
                     /locus_tag="IVW84_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916773.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35656..36492)
                     /locus_tag="IVW84_000548"
     CDS             complement(35656..36492)
                     /locus_tag="IVW84_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7916774.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36494..37177)
                     /locus_tag="IVW84_000549"
     CDS             complement(36494..37177)
                     /locus_tag="IVW84_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7916775.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12013 a         6553 c         7638 g        11040 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tctacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctgaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa aaaagccaat
    21241 gtaatgaaat aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact
    21301 tgaggcaagg cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg
    21361 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata
    21421 ctcgaaacta aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc
    21481 tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa
    21541 tactcaggtc agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat
    21601 ctgcggtaat aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct
    21661 tattacttga tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa
    21721 aaaggcaaat cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag
    21781 atcgtgccgc ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc
    21841 atccgtttga ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc
    21901 aattcctttc catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca
    21961 tattcaccgg ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg
    22021 gccatacttc cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga
    22081 gtcatcaact gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag
    22141 taaggatagt gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc
    22201 atctcactca tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga
    22261 ttgcacagct caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt
    22321 cctgaaaaat cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct
    22381 tgtccccaac gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc
    22441 ataaatcctg actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc
    22501 ttcgtcattt cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa
    22561 gcatagtcta atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc
    22621 aagtaatctt ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct
    22681 ttttccattg ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca
    22741 tctgcaatga ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc
    22801 cattttggca aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg
    22861 tatttttcta cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac
    22921 ccattatttg tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt
    22981 ttttgcccac taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa
    23041 ttatgataaa acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa
    23101 taggactgcc attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct
    23161 agttccaggt tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt
    23221 tgtttttttg tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct
    23281 aactcatcaa ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga
    23341 tcttctgaac cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag
    23401 agctgatggc gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata
    23461 gataagattc cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga
    23521 cagatcgaca gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca
    23581 tggaaaaggg tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa
    23641 tcacccatcc aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca
    23701 ggatgaaacc accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca
    23761 aaaggaacgc cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt
    23821 gcatgtctag tatctatttg accgatattc attttatttc tcctttgttg attgtctttt
    23881 gatgaatgaa actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac
    23941 caaagcatct cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg
    24001 cgggtcaaca agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg
    24061 gatatgataa ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc
    24121 acaaacaaaa gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc
    24181 gcttttgtca tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta
    24241 accaaggtaa cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat
    24301 ttttgaatgt ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc
    24361 ggaaagaacg gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa
    24421 ttctttcaat gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata
    24481 aatcaatccg tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag
    24541 cgtcgtcatc atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa
    24601 atcaccaaca gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt
    24661 aggagaagca agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac
    24721 gcgataaata agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct
    24781 tactttatag gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc
    24841 accagttaaa atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat
    24901 ataaacacct cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa
    24961 taaaaagata ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag
    25021 atatatcaaa tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc
    25081 agaaatttat ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga
    25141 attttacagc agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa
    25201 ccaccttttt attgacggga gacattccag cgatgtggtt aagagactct actgcacaag
    25261 ttcgtcctta tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt
    25321 tagtcaaaaa gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag
    25381 aagctaacgg tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa
    25441 gaaaatatga aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa
    25501 atacaggaaa aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg
    25561 acgtgttcaa aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga
    25621 caagaaaaga agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa
    25681 tgacatggtc tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat
    25741 ccaatatgtt tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg
    25801 atgataaaga aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc
    25861 agaatcacgg acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg
    25921 gaatgggaaa cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt
    25981 acttgggata ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga
    26041 gtaaagaaaa tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata
    26101 cgccagaaaa atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca
    26161 aatctgaaaa agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta
    26221 tgcacgaagg attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg
    26281 caaatatgat gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg
    26341 aggtatcgta aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat
    26401 ggtacatggc ttacgaacag catcacatgc gactgattga attgatagac gatctactag
    26461 agctatttga agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt
    26521 tagatgacta tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg
    26581 aaggaaaatt gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg
    26641 aatcgaacgt gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg
    26701 tcatgttagg ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga
    26761 aacaagctgg tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata
    26821 atcaagtttt ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc
    26881 ctgatcaaac agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga
    26941 ttccagtaga aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac
    27001 aatatgcctc tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa
    27061 aagatatatc aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc
    27121 attctaattt tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct
    27181 cagtagaagg agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag
    27241 catctgctcg ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata
    27301 ttacagaacc acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat
    27361 tagattatgc ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt
    27421 cagtcgattc tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa
    27481 agtatcttgc acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc
    27541 caaaagacag ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag
    27601 cagaaataac catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat
    27661 atcaagagtt agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca
    27721 ctataccagc tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc
    27781 gtttccgtgt gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc
    27841 cagcattttc ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg
    27901 aaacaatgat tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg
    27961 aaaaaaatgg taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc
    28021 atatttttga agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg
    28081 atcagccaat cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac
    28141 cggaaatagc aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc
    28201 gtttagaaaa agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca
    28261 aagaaaaacg aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa
    28321 tcgactttga aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc
    28381 ctacaaagtt acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc
    28441 caaaccatgt gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg
    28501 tcaatattca tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg
    28561 aagtaacaga agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg
    28621 attggggata ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca
    28681 gcattgaact acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac
    28741 agattccttt ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac
    28801 aatatttaac gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta
    28861 gtgataaagt agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa
    28921 ctaaagacaa tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg
    28981 ttgtcccaat aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat
    29041 gtatggcgga atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga
    29101 aattttagaa aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt
    29161 tgaatttttt gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg
    29221 tatcgacaaa acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc
    29281 acaattcgat tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac
    29341 ggatgtcaat gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag
    29401 ttgtatttac cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat
    29461 tttttcaggg atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga
    29521 ccattatgaa ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc
    29581 gtctattgag gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg
    29641 ggctattcag gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc
    29701 tgaaaagatt attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg
    29761 tcagcaattt gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat
    29821 cgttcgttgg ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca
    29881 cgaacatcaa aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc
    29941 tgataagact ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact
    30001 attttttctt gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt
    30061 tcagctgttc tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa
    30121 tgaactctga tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca
    30181 gaaagtactt tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg
    30241 accctttttc tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg
    30301 gcaatttctc cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt
    30361 cttccaagta tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct
    30421 ttgtccaagc ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa
    30481 tgattgagta aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc
    30541 ttatccacta taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct
    30601 aattgaactt cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg
    30661 ccctttactt catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata
    30721 cgcaaaaagt gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt
    30781 gttgtttcag gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca
    30841 gtgcaattgc agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt
    30901 tctgttcatt cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac
    30961 ttaagcagga agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac
    31021 aggagaagaa gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca
    31081 gtttttggcg ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct
    31141 ggaaaaaata tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga
    31201 caagtatcta cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat
    31261 taagccaatc gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc
    31321 aaaaaggaac atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct
    31381 cgacagaaga taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg
    31441 cagcagtccg aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc
    31501 agcagcttgc caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg
    31561 aattattttt ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt
    31621 atcttaacaa tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt
    31681 attttggagt agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc
    31741 tgaaaggtcc tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa
    31801 acacagtact aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag
    31861 aaagccagac agatatcagc gcgtatctta atgacacata cgataattca gattctggtt
    31921 atcgttatcc ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg
    31981 atgaagaaga tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc
    32041 aaaagcaatt agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg
    32101 gtgccatggt ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac
    32161 tagtaggacg aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc
    32221 gttcaccagg atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat
    32281 acaagccttc cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg
    32341 acgggacata ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc
    32401 ctgctgtatg gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat
    32461 tcggcctgcc tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa
    32521 aaggagtatc tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga
    32581 tgtatacgcc gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata
    32641 acacaaacaa aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca
    32701 tgcttcttgg tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga
    32761 tagcagggaa aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat
    32821 ggatgatcgg ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca
    32881 gcaagctgca ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag
    32941 cggcagaagg catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg
    33001 ccacaggagg acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag
    33061 agtctggaaa ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga
    33121 aaaacttcgg cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc
    33181 tctggcggaa atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata
    33241 gagagtaact actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat
    33301 caaatcgaac gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag
    33361 aaagtacaag caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc
    33421 gaattacaag aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca
    33481 caagaaatgg ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag
    33541 caaggcttca gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac
    33601 ctttacagcg aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc
    33661 ttgagcgatg acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc
    33721 aagaggtcgt gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct
    33781 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
    33841 ttcagaaata ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt
    33901 ttcaagcggt agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg
    33961 atttttcctt catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga
    34021 gccacaataa aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc
    34081 tctgactggt tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt
    34141 atcaacgctc gtgcaatgga gatacattgc ttttggccac cagacagctt gttgccatgt
    34201 tcgcctacta aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca
    34261 gcttcgatga cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc
    34321 tctttgatcg aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc
    34381 acacttcgca tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc
    34441 ggctggataa atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca
    34501 acaaaagcaa ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg
    34561 agtgagtccg gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg
    34621 atttttttct taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa
    34681 gtgatagatt ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa
    34741 ttgacgatcg tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct
    34801 tgtaaagcta atatcccagt gaaactcaaa caaatcagct gaaatatctg aaaactagcc
    34861 cagctgactg atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt
    34921 tgagtaaaat gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca
    34981 atcggtgcta attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg
    35041 aagtctgagt tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga
    35101 ggaacagtca ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa
    35161 gctacgaata aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt
    35221 tggatagctt ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt
    35281 tgataaggaa tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct
    35341 acttgacgga tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga
    35401 ccaatcagac aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct
    35461 ttcgatgaag ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga
    35521 tgtttgataa taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga
    35581 tgcagcaatg tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc
    35641 tccataatct tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg
    35701 aacataaatc tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat
    35761 ataatgcagt accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt
    35821 tccattttcc atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc
    35881 caaggcacct cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc
    35941 acaatctaaa atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa
    36001 ctgatagtta tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt
    36061 ttctgcatga gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt
    36121 aaaattttct tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc
    36181 ttgataggca gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac
    36241 ttcttgattt ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt
    36301 tttttggaag tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag
    36361 ttgttcagga aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac
    36421 tttttgaacg atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt
    36481 taatggaaac atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt
    36541 ttatcaattc aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat
    36601 tgtcttttag tccttctggg taatttttac aagtatcagg cataagatgc aggtagaatt
    36661 tttttgtctc ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta
    36721 aaagaggaat atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta
    36781 tatctggatt caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga
    36841 ccatttggac gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc
    36901 taccgaaggg aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta
    36961 caaataagaa atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga
    37021 taaatgattt ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa
    37081 aataagcaga gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg
    37141 ccgcagtaga atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt
    37201 ttataggcaa tgaagcagaa cagcaagaga agaagacgga gcaa
//
LOCUS       DACCWV010000012        36570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCWV010000012 DACCWV010000000
VERSION     DACCWV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.012"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IVW84_000550"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IVW84_000550"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7916776.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IVW84_000551"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IVW84_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7916777.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IVW84_000552"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IVW84_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7916778.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IVW84_000553"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IVW84_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7916779.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IVW84_000554"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IVW84_000554"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7916780.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IVW84_000555"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IVW84_000555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7916781.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IVW84_000556"
     CDS             complement(8054..8524)
                     /locus_tag="IVW84_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7916782.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IVW84_000557"
     CDS             complement(8570..8995)
                     /locus_tag="IVW84_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7916783.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IVW84_000558"
     CDS             complement(9062..9331)
                     /locus_tag="IVW84_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7916784.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IVW84_000559"
     CDS             complement(9342..10937)
                     /locus_tag="IVW84_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7916785.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IVW84_000560"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IVW84_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7916786.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IVW84_000561"
     CDS             complement(14490..15050)
                     /locus_tag="IVW84_000561"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7916787.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IVW84_000562"
     CDS             15403..16377
                     /locus_tag="IVW84_000562"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7916788.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IVW84_000563"
     CDS             16547..17947
                     /locus_tag="IVW84_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7916789.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IVW84_000564"
     CDS             complement(18025..18747)
                     /locus_tag="IVW84_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7916790.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IVW84_000565"
     CDS             complement(18826..19332)
                     /locus_tag="IVW84_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7916791.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IVW84_000566"
     CDS             19455..21254
                     /locus_tag="IVW84_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7916792.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IVW84_000567"
     CDS             complement(21322..22896)
                     /locus_tag="IVW84_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916793.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IVW84_000568"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IVW84_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7916794.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IVW84_000569"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IVW84_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7916795.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IVW84_000570"
     CDS             complement(24673..25311)
                     /locus_tag="IVW84_000570"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7916796.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IVW84_000571"
     CDS             complement(25312..25962)
                     /locus_tag="IVW84_000571"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7916797.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IVW84_000572"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IVW84_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7916798.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IVW84_000573"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IVW84_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7916799.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IVW84_000574"
     CDS             complement(29105..29845)
                     /locus_tag="IVW84_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7916800.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IVW84_000575"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IVW84_000575"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7916801.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IVW84_000576"
     CDS             complement(31216..32154)
                     /locus_tag="IVW84_000576"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7916802.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IVW84_000577"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IVW84_000577"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7916803.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IVW84_000578"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IVW84_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7916804.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IVW84_000579"
     CDS             complement(35314..35619)
                     /locus_tag="IVW84_000579"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7916805.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IVW84_000580"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IVW84_000580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7916806.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6706 g        11989 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaat
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DACCWV010000013        32365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCWV010000013 DACCWV010000000
VERSION     DACCWV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.013"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            167..3394
                     /gene="fss3"
                     /locus_tag="IVW84_000581"
     CDS             167..3394
                     /gene="fss3"
                     /locus_tag="IVW84_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7916807.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3506..3820
                     /locus_tag="IVW84_000582"
     CDS             3506..3820
                     /locus_tag="IVW84_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7916808.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3833..4207
                     /locus_tag="IVW84_000583"
     CDS             3833..4207
                     /locus_tag="IVW84_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7916809.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4217..4561
                     /locus_tag="IVW84_000584"
     CDS             4217..4561
                     /locus_tag="IVW84_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7916810.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4630..5646
                     /locus_tag="IVW84_000585"
     CDS             4630..5646
                     /locus_tag="IVW84_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7916811.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6240..8114
                     /gene="ltrA"
                     /locus_tag="IVW84_000586"
     CDS             6240..8114
                     /gene="ltrA"
                     /locus_tag="IVW84_000586"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7916812.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8240..8596
                     /locus_tag="IVW84_000587"
     CDS             8240..8596
                     /locus_tag="IVW84_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7916813.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8641..9783
                     /gene="dcm"
                     /locus_tag="IVW84_000588"
     CDS             8641..9783
                     /gene="dcm"
                     /locus_tag="IVW84_000588"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7916814.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9773..10062
                     /locus_tag="IVW84_000589"
                     /pseudo
     CDS             9773..10062
                     /locus_tag="IVW84_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10318..11502
                     /locus_tag="IVW84_000590"
     CDS             10318..11502
                     /locus_tag="IVW84_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7916815.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11517..11612
                     /locus_tag="IVW84_000591"
     CDS             11517..11612
                     /locus_tag="IVW84_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7916816.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            12382..14292
                     /gene="ltrA"
                     /locus_tag="IVW84_000592"
     CDS             12382..14292
                     /gene="ltrA"
                     /locus_tag="IVW84_000592"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7916817.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            14396..14620
                     /locus_tag="IVW84_000593"
     CDS             14396..14620
                     /locus_tag="IVW84_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916818.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            14633..15136
                     /locus_tag="IVW84_000594"
     CDS             14633..15136
                     /locus_tag="IVW84_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7916819.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            15263..16300
                     /locus_tag="IVW84_000595"
     CDS             15263..16300
                     /locus_tag="IVW84_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916820.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            16315..18012
                     /locus_tag="IVW84_000596"
     CDS             16315..18012
                     /locus_tag="IVW84_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP7916821.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            18005..18466
                     /locus_tag="IVW84_000597"
     CDS             18005..18466
                     /locus_tag="IVW84_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916822.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            18479..19339
                     /locus_tag="IVW84_000598"
     CDS             18479..19339
                     /locus_tag="IVW84_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916823.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            19377..19766
                     /locus_tag="IVW84_000599"
     CDS             19377..19766
                     /locus_tag="IVW84_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7916824.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            19753..22200
                     /locus_tag="IVW84_000600"
     CDS             19753..22200
                     /locus_tag="IVW84_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7916825.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            22205..24313
                     /locus_tag="IVW84_000601"
     CDS             22205..24313
                     /locus_tag="IVW84_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7916826.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            24310..25314
                     /locus_tag="IVW84_000602"
     CDS             24310..25314
                     /locus_tag="IVW84_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7916827.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            25333..26244
                     /locus_tag="IVW84_000603"
     CDS             25333..26244
                     /locus_tag="IVW84_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7916828.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            26431..26718
                     /locus_tag="IVW84_000604"
                     /pseudo
     CDS             26431..26718
                     /locus_tag="IVW84_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(26906..27121)
                     /locus_tag="IVW84_000605"
     CDS             complement(26906..27121)
                     /locus_tag="IVW84_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916829.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(27380..27547)
                     /locus_tag="IVW84_000606"
     CDS             complement(27380..27547)
                     /locus_tag="IVW84_000606"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7916830.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(27892..29331)
                     /locus_tag="IVW84_000607"
                     /pseudo
     CDS             complement(27892..29331)
                     /locus_tag="IVW84_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(29879..30550)
                     /locus_tag="IVW84_000608"
     CDS             complement(29879..30550)
                     /locus_tag="IVW84_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7916831.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(30899..32350)
                     /locus_tag="IVW84_000609"
     CDS             complement(30899..32350)
                     /locus_tag="IVW84_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7916832.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT        10823 a         5445 c         6625 g         9472 t
ORIGIN      
        1 tctaatttaa aggcagtata tttcgtattt tatattaaat ttttaggctc attcattctc
       61 aaatccattc tatttagtca ataaatattt taaaaagact ttataaacaa agatgttcaa
      121 gcgagcatct tatttttttg tgcctaattt gaaaggagaa catttcatgt ataaaaaaca
      181 aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaatcaa ttgtaggagc
      241 aattgctcct acgattgctt ttgctgatga aattacccat ccacaaacgg taaccgtaga
      301 actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc
      361 agaagtcact gttccgcatt atgcagtgta caacggcgtt aaacaagata ttttctgtgt
      421 tgaaccagga gtcccgattt acaatgagtt cactcctgga tatgagaaga acccgttacc
      481 agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc
      541 agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca
      601 ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaatcaa
      661 tcaagccatt gccgattatc agaagaaacc aagcttccat aatagtacgg ctaaaacagt
      721 attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga
      781 ggttgtggaa aataccgcaa acattgatta tcgtgtgaat ggcaatcagt tgataatcag
      841 cccaaatgcc aattcaaaag aaagtggtgt gttgacgctt aagaaatctg ctggtaccgg
      901 aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg cgatcgataa
      961 gccgaacact tatacggtca aaattgatgt ggaaatggag ggttctttga agattaaaaa
     1021 agttgataaa gaatcgggtg atattatacc aggaacggtt ttccatttag actttggaaa
     1081 aactttaccc gcaaaagacg tgaccaccga taaagaaggc attgcgacct tagatgagat
     1141 tcctcatggt acaaaggtaa ccattactga aaaatcggtg ccggcgcctt atacgattga
     1201 taccacaccc atgactgcca caattaaagc aggtgaaacc atttcagtga cttcaaaaaa
     1261 tacgcgagaa aaaggtcaaa ttattctgga taaaaccgga attgaaacag ggagtgatct
     1321 ttggaacgat aattactctt tagcgggcaa tacgtttgcc attcgcaagg atagcccaac
     1381 tggtgaaatc gtccaagaaa tgaccacgga tgaaaaaggt catgcagaaa caccaaaaga
     1441 gattgccaat gcgttggaat tgggaaccta ctatgtgact gaaaccaagg ctagtcatgg
     1501 ctttgtgaat actttcaaac cagtaaaagt cgaattgaat tatgtcaatc aaacagtggc
     1561 tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatt actggtgaaa ccactttgac
     1621 aaaagaagac aaagataccg gtcatgaaac tcaagggaaa gctgagttta aaagagctga
     1681 atacaccttg tttactgtta aggatggaaa agcggtcaaa tggaccgaga actttaaacc
     1741 agaattgttg aagggaacga aagcttctga tgaaaccgta actttgactt tagatgaaaa
     1801 gaatcaagtt gccgttaaac acttagcgat taatgagtat tactggcaag aaaccaaagc
     1861 gcctgaagga tataccttgg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga
     1921 tagcgaaaaa aatgctgtga ttactcgaga tgttgcggca aaagaacaag tgattcgctt
     1981 tggctttgat ttctttaaat ttgctggatc aactactggc actgccgaaa ctggcttcaa
     2041 tgatctaacc tttaaagtgt cgccattgga aggaaccaat gaaatcaccg gtgccgaaga
     2101 tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa
     2161 tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat
     2221 tacgccatta gaaatccgtt ctacttttaa agaaaataaa gaggattata ccaagagtga
     2281 ttatatcttt actattaccg aaaaagggca aaagcaaccg attaaaacgg taacggtccc
     2341 ttatgggaat ctaaccaatg agaatttctc agtcagtttg aaccgtttga tgctttatga
     2401 tttgcctgag gaagaagata gcttaacttc tcttgcgact tggaaagaag gagataaaga
     2461 attgactact cttgattcta ccgagctagt cgataaattg agctataatc tacatgaaat
     2521 caaagaagac tggtatatcg tagctcaagc catcgatgta gatgctacaa aagctgccca
     2581 agaaaaagat aaaaaagcca aaccggtggc gattgcggaa acaaccgcaa ctttagcgaa
     2641 taaagagaaa acaggaactt gggagattct gcataaatta actgtcgaac aagttttaga
     2701 caagaccatt gtcttattca actatgtgta tgaaagcaaa gaagcctatg aagctggtga
     2761 tgagccggta gcgaaggatg ctagcttgaa caatcaagcg caaactgtta attgtatggt
     2821 ggaacgtcat gtttccatcc aaaccaaagc gcacttagaa gaaggttctc agacctttac
     2881 tcatggtgac gtggtagata tgtttgatga tgtatccatt acccatgatg tactagatgg
     2941 ctcaaaagaa gcttttgaaa caattctgta cgctttattt ccagatggta caagtaaaga
     3001 aatttggaaa tctggcaaga ttgattacga agtgaatgac aaagaattta ccaaaaccgt
     3061 acttgcggaa aaagtggata caagtaaata tccagaaggt acaagtttta ccttcaaaga
     3121 aattaactat gacaaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa
     3181 atctcaaact ttaacgccca aaaaagttcc gacaacacca agtacaccag aacaatctga
     3241 aatgccaact gttccaagtg gctctcaaga atctagtcct acagtgaagg cattccctca
     3301 aaccggggag aaaacttcca atgtgctact gttcattggc tttagcttga tctttgcgac
     3361 ggctggctat tatttttgga atcgtcggaa ctaaggtgat aagatgaaac atgtaaatag
     3421 tagaaaacaa tacgcgcgac cgcctcctaa aaaagtggtc gcgctatttt tgtattcaaa
     3481 actaacaatg aaaagaggaa aaaatatgga attgaaattt gttgtaccag atatggtcga
     3541 gacgttcgga aaactaacat acggtggtga aggtgaagtt ctatcggaag ggtatggtcg
     3601 aaatactaca attgttggtc gaagttacca tctgttttca aataaacaac gggccgatga
     3661 tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgaccaag atcaaccact
     3721 aaaagctgta aatccccact taattgccaa aggctatgaa attgagaatc gtgggtttac
     3781 cgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt
     3841 aacagaaggt attgtcgtag attcaggatt aacctttggc aagttgcgtt tttccgcatt
     3901 acgtcgagaa gtacgcaagc aaaatgagga cggaacggtt agtaacgagg taaaagaacg
     3961 gacctataat ttgaaatcct ccgcgcaagg tcgaatgatc caagtcagta ttccagcaaa
     4021 tgtaccctta cgcgagtttg cttatgatgc agaggtagag ctggtcaatc caatcgtgga
     4081 tacggtcgcc aattatgttt ttcgggaagg aaccaccgtt aattggttta tcaaggcaga
     4141 tgatttagtc ttgaaacgac aactgaatca aggaaaacct acgaaccaaa atgaaggaaa
     4201 gaaataggtg aagaatatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaatt
     4261 tggcaaaggt gagctaacgg aggaacaact cactaacgca ttaacctatg atgaaaaaat
     4321 gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgccaaag aagactttga
     4381 tgtactggat tggttagggg aagaagagtc tttctttatg gttgtcgagg taaatgaaag
     4441 tttatgtcgg caggcagaat ctgttttgga agaaatcgat gtggagatgc cggatgccat
     4501 cgaaagattt ttaaaacaat tagtggaaac gaaacaactt cccgtagtag taaatgacta
     4561 gcataacttt ttgaaattgc ccaatgaaat cctattgggc tttttcgact ggaggtggaa
     4621 accatcaaaa tgtacaaagg acatcgcata cgcgcaggag atcaacactt gatttatcac
     4681 tttgttcttg gctggctaat ctttttgttc attggttgga tgagcgtctt ttatttacaa
     4741 gaacttagac aatttgatat ttctaaggta tcattttcca ctagtgaaat tggctggtct
     4801 atgaaggatg taatctgctt gctaggaagt ctcgctcttt caggaagcat gatgttactt
     4861 tatatacact tttttcagga tcattggcga agtctttggc atcgacaaaa gttagctcgg
     4921 atgattctag aaaaccattg gtatgaagta aagcagactc aaggtgaagg ttttttcaaa
     4981 gatttgaata gtagttggac caaagagagt atcagttact tccccaaaat ttattatcga
     5041 atgaaagatg gcttactttc tattcgcgtt caaatttcac tgggaaaata tcaggatcag
     5101 ctattgaagt tggaaaataa gttagaaagc ggactgtatt gtgagttagt agaaaaagaa
     5161 ctcaaagact cctatgtgga atacaccttg ttatacgata tgattgccaa tcgaattggg
     5221 atagaagaaa tagtggcaga aaatggggcg ttgcgattga tgaaaaatca agtatgggcg
     5281 tatgattcct tgccgcatat gttaattgct ggcggcacag gtggtgggaa gacctatttc
     5341 ttactcacca ttattcaagc actattgaag tcagatgcag aattgtttat cctcgatccc
     5401 aaaaatgcgg acctcgccga tttaggtacg ataatgcctc acgtatactc ccaaaaagaa
     5461 gaaatttctg aatgtgtgga agatttttac gaacgcatga tagctcgtag taggtcaatg
     5521 aaagaaatgc ccaactacaa aacaggagag aattatgcgt atctcggact tccgcccaac
     5581 tttttaatct ttgatgaata cgtggcttat atgggtgcga atcgttttcc tacaagcatc
     5641 gaatagatgt gaaatggaag cctattgaga ttatcaatag tgacaactaa tgttttgaga
     5701 gatggaatga aagccttcaa gttgagctga aacatccccg ctaatactcc tacatgctct
     5761 aatcaatgaa gtagattggt tttggtatga agctaggtga agtcgtctga aatctgccta
     5821 agtgcttgag cataaggctg actatagggc gggcggctga aaaagataac tatggtaaga
     5881 atgtctgaaa atgactgacg aatctgcgaa tgtacgggtc taaaaggctg atcttattga
     5941 aagataggaa catacggacg tatggtgaga taccgacaag taaggttcca tgatatgaaa
     6001 atcgggatag ctaacgaaga agctataatt tcacaggctc atagtagata cctaaggtta
     6061 tacatgatag ctaaaaacat tggaacgatg taagcagaaa actatcaatt aaatgactac
     6121 ttctagatag gtgcatataa gtaataacga aattgctttg tttctgtgaa agtaagggca
     6181 cagtaccgat gaagcgtcta acaaatgtgg agggatagcc ctaagtctat ttaataacaa
     6241 tgaatgttga aacagatgaa gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg
     6301 aggtgccacg cttgaacaca aaaatacgtt actgggaata ttatgatcta caagaaacat
     6361 tcgatcagct atttactcaa agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa
     6421 tcatttctga aaataatatt cttttagcat accgtactat caaggcaaac aaggggtctt
     6481 cgacaccagg aacggattcc ttcacaattg ataactataa ggaaatgaac caagcagagt
     6541 ttatccatct tattctcagt catttagaaa attacaaacc aaaatccata aagcgagtaa
     6601 tgatacctaa acccaatggt gagaaacgcc cattaggaat tccatgcatg attgatcgaa
     6661 tgatccaaca gatgttcaag cagatactgg agccaatatg tgaagctaag ttttatgaac
     6721 atagctacgg tttcagacca ttgagaagtg caaagcatgc tctaggtcgc attatgtatc
     6781 tcataaacat cagtaaaatg cactatgcag tagacattga tatcaaaggt ttctttgata
     6841 atgtaaacca ccgtttactg ataaaacaat tatggaatat aggaatttgt gacaaacaag
     6901 tcttagctat tctttcaaaa tctctgaaat cacccattca aggagaaggt atcccgagca
     6961 aaggaacgat ccaaggaggt atcatttctc cactattatc taatgttgtt ctaaacgact
     7021 tagaccattg ggtctcaaag cagtggcata cctttgaaac aaagtatccc tatactaaag
     7081 gttacaataa atttcgtgct ctaagagata caaatctaaa gcaaggctat attgtcaggt
     7141 acgcagatga ctttaaaatc atgactaatg attatccgac agcattaaaa tggttccacg
     7201 ctgtcaagct ttacctaaaa gacagactta aattagatat ttccaatgag aagtctaaaa
     7261 tcgtcaattt gcgtaaatgt aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa
     7321 aagggaaaaa atgggtttgt aattcacgta tttctaacaa aaaaaaggat cagattaaag
     7381 aagagattaa acaaagaatc aaagatattc aaaagagtcc tacagcacaa aatgctctcc
     7441 tgtttaatgc tctcgtgctc ggtgttcata attatttcag gtatgccact catattaatt
     7501 tagatttgaa taggatgaac tacgttcttt cattcactac ttacaaccga ttatcaaatt
     7561 gtagtacaag atgttacccc aacaaagctt ccccaactta caaaaagttt tactcaacaa
     7621 cgatgaaaac gtataaagta gctggagtct atttgtaccc tctttgcgac gtatctacaa
     7681 aaacaattta cggatttaat acggaggata caccgtttac tccgtttggt cgtcaaagat
     7741 tggagcacaa aagcctacag tctcttgtgt tccaagaatt aagaaaattg atggaatcga
     7801 aaataccgaa tagaacggtt gagtatttag atagtagaat atctcgttac tcaatgaaat
     7861 caggcaagtg tgagattaca aaacaatttt tgccagcaaa agccgttcac tgtcatcact
     7921 atttgccaaa aagtcttggt ggagacgaca aattcgataa cttgagaatt attcataagg
     7981 atatccatct tctgatccat acaacaaata aaatgataat cgatcactac gtgcatgaac
     8041 tgaaactggt gccagagcaa atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc
     8101 aaaacattca atagaaaata aatagatgga acgccgtatg aggtgaaagt ctcacgtacg
     8161 gtgtggagcg ggggaaaaga tggagattat atcaaattct tacctatcgc tatagatgtt
     8221 aacgacaaaa gaaagtgcgg tgattttgaa taagcttaaa caaatcgtga tgttaggccg
     8281 tcaatctggt ttctttctga ttctagcttg tcaaaggccg gatgcgaaat atctaggaga
     8341 tgggattcgc gatcagttta acttccgtgt ggctttgggt cgtatgagtg agcttgggta
     8401 ttccatgatg tttggagaag tcgataaaaa tttttttatg aaacgcatca aaggacgagg
     8461 ttatgtagat acagggggca gtgtgattag tgagttttat acgccacttg taccaaaagg
     8521 gtatgatttc ttaagggaaa ttagcaacat tgttggatta accgttcata cggaaagaga
     8581 aaataatagt gtgtagccta ggaatatcaa taatcctggg cttttttatt gaggtgattt
     8641 atgacatttt tagatttatt cgccgggatt ggtggctttc gcttaggtat ggaacaagcc
     8701 ggtcaccagt gtatcggttt ttgtgaaatt gatgaatttg ctcggaggag ttataaagca
     8761 atccatgata caaggaagga ggtggaaatg catgacatca caagtgtatc agacgaattt
     8821 gttcgatccc tcggaccagt ggacatcctt tgcggcggct ttccgtgcca agctttttca
     8881 attgcgggaa agcggcaagg attttccgat actcgaggta ctttattctt tgaaatcgct
     8941 cggttcgcca ctcttctcca acctaagttt ctattccttg agaacgtccg gggattgctc
     9001 aatcacgaag gaggggctac gttcgagacg atcctccgaa cgttggatgg attggggtat
     9061 gatgtggaat ggcaagtgct taactcaaag gcctacgttc cccaaagccg tgagcggatc
     9121 ttccttatcg gacattctcg agatgcatgt accgaacaag tatttcctat cattggatcg
     9181 tcttcaacat ctgatcaaaa catcagaaac ttggtaaata tcaatccttc caatcgagga
     9241 atgggtggcc aagtctatgg ttcagatagc gtagcaccaa ccttgaccgg tgatgaagga
     9301 attaaaattg ccttaccggt aaaggatggg atttctgtag cgggtatgtt gtcgggaaat
     9361 tttgaacaag gcaataggat ttatgaggta accggaacag cccccacttt gtcaacgaaa
     9421 caagggggaa cgaagattat gattcggaaa aatgaaacag aatatcaaga ggtgaagcca
     9481 ggggacagtg tgaacctggc ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa
     9541 caaagtgtgc ataccttact aacaggagac caacaagcgg tagtcacgga tcagtaccag
     9601 attcgaaagt taacacctag ggagtgctgg cggcttcagg gctttccaga ttgggctttt
     9661 gatcgagctt ctcaagtcaa ttcagatagt cagttgtata aacaggccgg aaactcagta
     9721 acagttccgg tcatttttga tattgctaga agattaaagg gggtaaaagc atatgattta
     9781 tgacgagttg attggagaaa tctactggat gattggcaag attcaggcag atccgaaatt
     9841 ggaagaagaa ctgtaccggc tcaattttga tattcgcaaa aacggggtga aagtccaggt
     9901 gattcgtatg tagtggatga ggaaacggat gctcgaatgg agctcaatca agtcatcggt
     9961 gaattggaac gaattgcgga ccttgccaaa gaacctgata tccgccaata cttgtttgag
    10021 ataaaagcag agttggaaat tgagggtatc accgcggagt aatagggaaa taacccactg
    10081 ataggcactg accgcggggg atttgcgaag cagaaatcct ccgtggtgct gtgcctgttc
    10141 atggacggcg gcagccggcc atgatgtctc accccccgta tctaacaggg gggtacaaaa
    10201 acacaaaaaa caagcaagta accataaaaa atgcttgttg tatcaaggat attaagtaat
    10261 ttgtataggt gttaactcat ttggaaaagt taacaccttg aatttggagg tgaaaacttg
    10321 gcgcaacgaa atttagatta tcggttatta aaagaacgac gcaacgaata tggcgtttca
    10381 caaaataaat tagctactgc ttgcggactt agccgcccat acctaaatca gatagaaaac
    10441 ggtggcgtaa tcgcctctac aaagaccatg agaaaaattt ttgatcaact ggaaagtttc
    10501 aatcccgatt tacctttaac gttactattt gactatgtaa ggattcgctt tcccacaacg
    10561 gatgtgcgga aaattattca agaaattctc catttgaaat tcgagtatat gcttcatgaa
    10621 gattacgcct tttactccta tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta
    10681 tctcatgaag aagataaagg tgtccttttg gaattaaaag gtcgtggttg ccggcagttt
    10741 gaaacttttt tactcgccca aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa
    10801 gccggtggcg tgatgaaacg cttggattta gcaatcaatg atcgagtggg gctactggat
    10861 attcctgatt taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttc
    10921 aaaagttacc gttccgggga actgttgaaa gctgatgaaa aggatggtat gggcaatacc
    10981 ttatatattg gcagcctcaa aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa
    11041 caatatatca agttagggat tccattggac cagaccgaaa cgaaaaatcg ttttgagatt
    11101 cggttgaaaa atgaccgtgc ttattatgcg attcaagatt tgttaaaagg tcgtagcatt
    11161 gaaagtacca cgttttccat tatcaatcgg tatttgcgat ttgcggataa agtagatgga
    11221 aaacggcgaa ccaattggcc cttgaatgaa caatggggtc gttttattgg acggaatcgg
    11281 aaagagattc aattaacttc tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg
    11341 ctaggtagac aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcaa
    11401 acaacgtata tccaggatat ggtacagaat gcgcgactct cggaccggca taaaaagatt
    11461 ttggagcaac aaagtatggc aatcgaaaac ttgattgtat gaaaagagga attttgatgg
    11521 aaattgtaaa agatatgtta ttggtacttg gtggcagtat gcttggtgtt accgtgatgt
    11581 gcttgatgaa tgcaagtgca gcagcggatt gagcaatgga aaagaaagga aagtgacaaa
    11641 atgaatttcg gacaaaattt atgtgcgact cgttccttag tgaaaagcta aggcactaat
    11701 aaaagaacga aaaagtttgg ttattaggag aactgataat ctgacaggtg gaatgatgaa
    11761 gtaacgctct gaaacgcctg ccctgagcct ccgacaagca ttgtatgagg aaactcagaa
    11821 tgtttgaagc tcggtaaaga cggctgaaag ataccgtaat agttgatgaa catatatctt
    11881 ctatcgaaaa ctatccagag gtgggtagcg tatccaacag gtcgggggtc tataaaatat
    11941 ctatggttag aatgttctat aaggaactga cgaaacagcg aatgtacggg tctaaatttg
    12001 aactttgtag aaatgcaaag gtacgagaaa tcgtagctgg tgtggcaaag taagaatata
    12061 ggttatgaaa tgccatataa cgttacaggc ggtatcaagc cagcaggctt atagctagtg
    12121 acctaaggat atatatgtat agataagatt atcggaacga ggaaaggtat tgggttctca
    12181 tcaagagaat aaactgacga agaacaataa gcagttgaac taatgctgaa aagcagaggg
    12241 cgaacctaag aaattcttgt aatgagaatg gaggaatgcc ctcaagtcgt gtatatatta
    12301 gaaaagtatt atgttgtttc aataaggatt acgagtaaga taaaaaagct cactcaattt
    12361 caagtaaagg agtgaagcct tatgcctaaa aataaaaatg aaacattgtg tgtagaagat
    12421 ttaagacacg ctgaatatta cgaaatgcaa aacacctttg atgatttata tgccaaaagt
    12481 aagaatggag atattttcac tcatcttatg gatattatcc tatcgagaga aaatatatta
    12541 ctggcatatc gaaatatcaa agcaaatgca ggcagtaaaa cagcaggaac agacggtaca
    12601 ataattaaag atattggcaa attgccagca gaaacagttg tcaaaaaagt aagatacatt
    12661 gttgcaggca gtccacatgg gtatcgacct aagccagtga ggcgaaaaga aataccaaaa
    12721 ccaaatggta aaaccagacc tctaggtatt ccttgtatgt gggacaggct tatacaacaa
    12781 tgtataaaac aagtattaga accaatctgc gaagctaaat ttagtgaaaa cagctatggt
    12841 tttagaccta ataggtcagt tgaaaatgca ataaaagcaa cctataaccg cctacaaatc
    12901 agccagctac attatgtgat tgaatttgat attaagggtt tctttgacaa cgtgaaccat
    12961 tccaagctga taaaacagat atgggctatg ggaatacggg acaagcactt gatattcatt
    13021 ttaaaaagga tattaaaagc tccaatcaaa atgactaacg gtacaataac atatccagag
    13081 aaaggaactc cgcaaggggg aataatatcc ccactactag caaacattgt tcttaatgag
    13141 ttagaccatt gggtagaaag ccaatggcag gaaaatcctg ttacaaaaaa ctatgtggtt
    13201 catatcaata aaagcggtag cccatgtaaa agcaacgctt acaaagaaat gaagaaaaca
    13261 aagttaaagg aaatgtatat ggttcgttat gcagatgatt ttagagtttt ctgccgctac
    13321 aaggaaagtg ctgaaaaagc caaaatagca atcactcaat ggattgaaca acgattaaaa
    13381 ttggaagtat cacaagaaaa aacaagaatt gtaaatgtta gaaaaagata ctccgacttt
    13441 cttggtttca agattaaaat gataccgaga agaaagaagt tagttgttaa atcacatata
    13501 agtgataaac aattcaaaaa ccaaaaagac aagctggtat cacaggcaaa gaaaattgct
    13561 tctccctcaa aagggaaaac ggaattagaa gaaacaagac tttataattc aatggtatta
    13621 ggtatgcaaa attattatca gattgcaacg aatgtaaatc tggattgtat gaaactgaat
    13681 agagctgtaa tggctatctt cactaacaga ttaggtacaa acaaaagggg cagattgaga
    13741 aaaacaggca gacaacttac gacaaaggag gcaacaagat atggtaaatc taaaatgctt
    13801 cggtatgttg ctggcgaggg cgaacctatt ttccctgttg gatatatcca acacagaaaa
    13861 ccaatggcaa aggtgtattt agcgaattgc tacactctag agggaagaaa atttatccac
    13921 accaatctaa gtgttgataa attactgatg taccagttaa tgaaattatc tttggaaaat
    13981 cgcacaatag aatacgcaga caatcgaatt tcgctatttt ctgctcaaca tggaaaatgt
    14041 gctataactc tggaagaatt tcaacaagct aatgaaatac attgtcatca tattgtacca
    14101 actggagctg gtggaaatga cgattataaa aatcttattt tggtaaaaga ggcggtacac
    14161 cgtctaatac acgctaaaac agaagaaaca atacaaaaat atatagctct attgaagcta
    14221 aataaagtcc agctcataag actaaataaa tatagagtat tagctggcaa tcaagaatta
    14281 aacttgatat aggtctacta cataaatact tttcaatata aacacgatgg aacgcggagt
    14341 gcggtgaaag tcgcacgctc cgtgtgaatg gggggaaaag tttgagatat ttataatatc
    14401 aggaacttac ctatccatat atcaatggtt tttaaccaat gctcaatctt tagtcttact
    14461 agcgattgtg gtcattggtt tgtttttagg atttaaacgg gagttttcca aactgattgg
    14521 cttcttggtg attgctttga ttgcggtagg tctggtcttt aatgcttctg gtgtgaagga
    14581 tgtcctactc aatttgttta accgaattgt cggtgcctga gggggaatca caatggaaca
    14641 gatgcgtgtt tatattgcga accttggaaa atacaatgaa ggtgagttgg tgggggcatg
    14701 gtttacccca cctgtcgatt ttgatgaagt gaaagaacag attggattaa atgacgaata
    14761 tgaagagtac gcgattcatg attatgaact gccttttgag attgatgagt atacgccaat
    14821 tgaagagatt aatcggttgt gtaacttagc ggaggaacta gaaggaacgc caattggtga
    14881 agctgcttct gagattcaac acgctttctt caattctttc gaggaaatgg tagagcatgt
    14941 ggatgatatt gtctattatc cagattgcga tgatatgagt gatgtggcgc attacttgat
    15001 tgtagaagct ggtgattttg gcgaagttcc agaacaattg aagccgtata ttgattttga
    15061 tgcttgcggt cgagatttgg agattggagg aaattatttg gttactagtc gtggagtttt
    15121 tgagtatcca gaatgaatca aacactatat atagtgtttg attagtatat gtaagcaaaa
    15181 aaacacaata tatagagtgc aaactgaagg gtaatgtgat ataatatttg ccgaagaaat
    15241 atttttgaaa tgaggtaata ttatggcaaa tattcaaaca agttttattg atttccataa
    15301 ttcaattaga ttggacgtag aagacaatac gctacttaag gactataaag accaagtaat
    15361 tgatggatta aaagattact tacctgatga tgtgaagttt gaaacttttt tacagggaag
    15421 ctattctgtt tataccggaa taaaaagctg tgatgaaaaa attgattttg acatcgatat
    15481 tgcagttgct ttcgaaattg atcatactgt ttatgaagat ccaagggagc ctaaactgtg
    15541 ggtaaaagag gcgctagttg aaatttttcc taatgctcaa gttaatttaa aagtaccttg
    15601 tgtaactgct acatttactg gaaaaaagac aaaaaaaaat gtgcacgttg atgttgcggt
    15661 ttatgctaaa gaagatgaaa attatttctt agcaaaagca aaagaattca gtgctcccga
    15721 aaaccgatgc tgggaggaag cggatcccaa agtattaaaa gagaaaatta atagtcatgt
    15781 agcagattca gatgatcgaa aacaatttcg aagatgtatt cgctatttaa aaagatggaa
    15841 agataacaat tttaaccaag aatataaacc aacaggaatt ggacttacaa ttaatgtgat
    15901 ggatacgttc ctagttaata agtccactga ttttttaact agaaaagttc agtataatga
    15961 tatggaatgt atgaagcaga ttgtttcttc gctgaaagat tcatttgtgt atgagtacag
    16021 tgaaacagat ggttggcatt atcgtctaca tgcatatctt ccagtgaagc ctaacagtga
    16081 cacctattca aaaatgacag tgaatcagat gagtgatttt aaaaacaaac tttctaaatt
    16141 atatgatgat ttgattttcg ctatcgatac tgaagatgag tatgaagcta caaaacgatt
    16201 aaataatcaa tttggggagg attttttaat tatttcagaa gaagaagtta cagaaaaaaa
    16261 cttaagaaat gcatttgtta cagactatcc gagtgcttag gttggtgtta aagattggat
    16321 tatactgata ttttaaaaga aaagctaaca gatgttgtgc gtttattcga tggtaattac
    16381 tatggggatt tttcatttgc tacaattgga aagttgcgtt taaaaattga aataccaaaa
    16441 cattttccat tggtacttcc taggatttac attcataatt ttgatcattt aggactttac
    16501 ctaccacatg tagaaaaaga tgggttggta tgtttttctt cagatataaa ttatttagta
    16561 gatgaagaaa attcagaatt ggttttattg cagtgtgtta ataaaactat aaaaacaatt
    16621 gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa aagagtttgt tgctttttgg
    16681 gacaaacaag agaatcaaat ttcgttattc tacctttgtg aaccagcgtt gtttatcaag
    16741 tcagttgata caataatgta cggtaagtct ttggttgctt tggataaaga gaacgaatta
    16801 caattaaatg cagcgagtag attgtttaat ggagagaaag aagatactca tgaattagat
    16861 gcatactatg ttccaattag aaacaagaat aatattatgc ccccgaagaa taataaagca
    16921 tggtcaaaga aggatatttt acaaatagta aaaaaaaatg taacaggtgc aaataaaaga
    16981 ttttttttaa aacttgaaaa aagaaagaat agacataatg ttttacttat tctagatatc
    17041 ccaattgaag aaaagtcaaa taatgtcata attggatttc tttttacaaa acaatctgga
    17101 tctataaaag attataaggg tacgcctagg actgtattag ttcagcgtca tgatgaatta
    17161 tatttggtga atagaacgag cggagaaaac tatggattgg gtgctaatat agctattgtt
    17221 ggggtagggt cagttggcag taaaattgct gaaaatcttg gtccattagg tgtgagaaag
    17281 atgacactca tagacaatga ttgttttcaa gctgaaaatt tgtatcgaaa ttcacttggt
    17341 gcagatagtt taaaaaatgc ccgatctcag aataattttt ttaaagttga tttaatgcag
    17401 gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag tggagtcaat gaacgtttta
    17461 gacttaatta gtatgcaacc tggttttttc aaggaatatg actatgtttt tgtttgcata
    17521 ggagatacaa tggcaagcct ggctttaaat aattattttt ccaaaagtag tcaaaaagtt
    17581 ttctattctt ggctggagcc atatggcatt gggggacaca ctctatttat aaattattct
    17641 ctaaatcaaa aaggttgttt caattgtctt tatacagatt caaactctaa aaataggtgt
    17701 tcaaatagag cttctctggt aaaagaagga caaatttttg aaaggacttt agcgagttgc
    17761 cgctcaaaat ttataccata taatgctatt gcctcgacgg aatgtgcaat taatgcaata
    17821 aaacttttcc ataaagtcaa tcaaaataat atgaccaata gtactctttt ttcatggata
    17881 ggagatggga gtacttttga gaatttagga tatcaatata gcgacagaaa tacttttctt
    17941 aatggagtca ataagtttga agaaagattc ttttctaaat attgcaaagt atgtggggag
    18001 ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa gtaagggatg aagttcttaa
    18061 gatatttgaa caatatcggc agggaaagga tgacttgcct gaagctggag gagttcttca
    18121 tggaaaaaga cttaagaatg ggagcataat aattaccgat ataacaacac ctcaaatagg
    18181 agatatacaa aaaagattac tctttaaaaa aaaccgagaa attcatcaac agctttctaa
    18241 tgagaaatgg atagaatcaa ataagttttc tattgcattg ggagaatggc atactcatcc
    18301 tgaagctgta ccaactccat caaatgttga taagaaaagc tggagactga acgtgagcaa
    18361 gcaacatgat gatagagttt atgtttttat tatcgttgga ttttctgatt tgagaatgtg
    18421 gttactgtca aaaaaatcag atattaggca ggctataaaa ctataaagga gtgattaggt
    18481 gaaatcacaa aagaatattt ggtggtgggg gtttttagta ggaaaggtaa tgataccagc
    18541 ccttctctta atttctctac cgatgattac ttttttcttt accaataaaa aaatgagtaa
    18601 agaagccgtg atttttttct ttggagacca aaaagctacc tttattgaaa cgttagtaac
    18661 atctcttcag cttaatttga attacatttt ttcaattatt attgttatat cgttattgaa
    18721 tttttttaaa aaaagaaatt caggaaaggt gtttaactct aatggaaatg tgtactacaa
    18781 ctatttttat tttgtatttt gggtagcagc aactttactt ggttatgata aaattcagat
    18841 tgcaggaatt cctatacaca tgcaatataa gttggttttg agtggaattt tttctgaggt
    18901 gttgccagat atatacgatg atcattacga cagtgatgga acatgtaaag taagcattga
    18961 aaaagaaaat ttcgatgaca tcgatggata tgactctgtg aacttactga ttattgatac
    19021 ttacgatata aaaatgagtg aattatctat ggaaaatcaa acatatccta cgataattgt
    19081 tagagggaat tcaatagatg gagttcgtaa agttaataga tctttaatct tggaaataaa
    19141 aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa gtatttgttg cttcaacatc
    19201 taatccaaaa aatagtatca atattattaa ttcatcgttt agatttttcg gtcgatcacg
    19261 taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc aatggaaagt atagtaagaa
    19321 atatagaatt tttatttaag aaggaatttt tccttctttt tattttggag gaaactatga
    19381 aaaaaataaa aagctacacc agtatttggg cagttgaaaa agtcatttat gcgattaatg
    19441 actttcagct gccttttcct gtgaccttta accagatggc ttggtttgtt ttatccttga
    19501 tgtttgtcat tgtatttgcc catgtgcctc cgctatccat gatagagggg gcttttttaa
    19561 aatacttggg cattccgata gcggtcactt ggttcatgtc gcaaaagacc tttgacggta
    19621 agaaaccatt gggattttta cgatccttta tcagctatca cttacggttt aaggtcactt
    19681 ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga cgaaccaatc accttggtaa
    19741 ggagtgtgaa ttatgtaccc gattaagtat attgaaaata atcttgtctt taaccaagaa
    19801 ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt actccttttt gtcacctgaa
    19861 cagaagtatc aagtacatga taactttcgc caattaattg cgcaaaatcg tgaaggtaag
    19921 atccatgcct tacaaattgc cacagaaagt agtattcgtg ccacacaaga acgttcgaaa
    19981 aaggaaatta ccggtagact caaagaagta gcaaagcaac gaattgattt acagacagat
    20041 gcattagtat ccatgattgg cgatagtcag attgattatc gcttctttat tggctttaaa
    20101 ctgatttcga cagatgaaga ggtcaatcta aagaatctca ggaaatcttt cttttctgga
    20161 cttcaagaat ttgtctatgg agtgaatcat catttgatgg gtgattttgt ttccttatcc
    20221 aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa tggaaagtaa attggctcga
    20281 agatttaaag tcaaacgagt aacgccgagt gacttgacgt atttaattga acatatctat
    20341 ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc taccaaagaa aaagctgaaa
    20401 tcagaaacgc tggtgaaacg ttacgactta ttacgtccca ctcgttgttt gattgaagaa
    20461 aagcctcggt acttacggat ggaacatgaa aatcatgagt cttatgtagc gtacttaaca
    20521 attaatacca ttgtagggga aatggagttt ccttcctcgg aacttttcta ttaccagcaa
    20581 caacaattta cgtttccgat tgatacttca atgaatgtgg aaattgtgac gaataaaaag
    20641 gccctagcta ccgttcgtaa taagaaaaag gaattaaaag atttagacaa ccatgcctat
    20701 caatctgata atgaaacgaa ctccaatgta ttagatgctt tggattccgt ggatgaattg
    20761 gaaaccacct tggatcagtc gaaagaatcc atgtataaac taagctatgt ggttcgagtg
    20821 agtgctaaat cagtggatga attaaaacgc cgctgtgatg aagtgctcga tttttatgac
    20881 gataccaatg taaaactagt ccggcccttt ggcgatatga tgggtctaca caaagaattc
    20941 ttaccctcaa gtaagcgata catgaatgac tatattcaat atgttacgtc tgactttttg
    21001 gcgggacttg gttttggggc tactcaaatg ctaggagaat tagaaggcat ttactttggc
    21061 tataacgtag atacggggcg caatgtttac ctgaaaccgg cattggcatc acaaggggtc
    21121 aaaggctcag taaccaatgc tttggcatct gctttccttg gttcgttagg tggggggaaa
    21181 tcttttagca acaatctctt agtctattat gccgtccttt ttggtgggca agctgtgatt
    21241 gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt tgccagaaat tgcggatgaa
    21301 attaatattc ttaacttgac cagtaagcca gaaaatcaag gcttacttga tccctatgtg
    21361 attatgaaga aaaagaaaga ctcagaaagt ttggcgattg atatcctgac ctttttaaca
    21421 gggatttcca gtcgggatgg ggagaagttc cctgtgttac gcaaagccat tcgaacggtt
    21481 acccaaagtg atcagcgagg attattacaa gtaatcgttg aattgcgtaa agaggactca
    21541 ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta ccgattatga ttttgcccat
    21601 ttgcttttct cagatggttc gattacccag tcgattagtt tagataagca gttaaatatc
    21661 attcaagtag cggatttggt tttaccagat gcagaaacca cttttgaaga atataccacc
    21721 atggaattgc tgagtgtttc catgctaatt gttatttcca cttttgcctt ggactttatc
    21781 catagcaacc gagaaatttt caagattgtg gacctggatg aggcttggag ttttcttcaa
    21841 gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg ctggtcgggc catgaatgcc
    21901 ggtgtttact tcgtcacgca aaacagtgat gacttgttgg atgaacgttt gaagaataat
    21961 attgggctaa agtttgcatt tcgcagtacc gatattcatg aaatcaagaa aaccttggaa
    22021 ttctttggat tagataaaga agatgaaagt aatcaaaaat gcatgcgaga tttggaaaat
    22081 gggcaatgct tgatgcagga tttgtatggt cgagtaggtg tggtccaagt tcatccagtc
    22141 tttgaagaac ttttccatgc ttttgatacc cgtccgccag ttcaagagag aatggagtga
    22201 ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt ttaatcagtt tggtcactct
    22261 gataatactt ttgagcttag ttggaacggt agctgaggca acaggtctgg ttgatgatac
    22321 agttggagcc ggaaatcttt attcccaata ttcacttggt aactatcagt tggatttctt
    22381 tgtggatagt tcgtgggatt ggctgccttg gaattggggt gatggcctag ggaagagcgt
    22441 aatgtatgga ctttatgcca ttactaattt tatttggaca gttagtctct acttatcaaa
    22501 tgccacgggc tacgtggttc aagaagccta taagctggat ttcatttcag atacggctga
    22561 aagtattggg aagaatatcc aaaccttagc cggcattacc gaaaatggac tccagacctc
    22621 gggcttttac tttgggtttc tattattgat gattttagct ctaggagttt atgtggcgta
    22681 tacaggatta ttaaaacgag aaactaccaa agcagtccgg gctgtcatta attttgtgat
    22741 gatttttcta ctttcgggtt cgttcattgc gtatgcgccc acctatatca caaaaatcaa
    22801 tgattttagc tcagatgtca gcgaagcggc tcttagcctt ggaaccgaga ttgtcgtgcc
    22861 gaattcagaa agccaaggaa aggacagtgt ggatttaatc cgagatagct tgttttccat
    22921 tcaagtccaa caaccgtggt tactcttgca atttgatgat tccaatatag aagaagttgg
    22981 cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat gaaaataaag gcaaggatcg
    23041 ggaagaagcg gtcaaagcgg agattgaaga taatgacaat gcgaatctca gtattaccaa
    23101 gaccatgaac cgcttaggga ccgtggtctt tttggtgttg ttcaatattg gcatatcctt
    23161 ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa attctcttta ttatctttgc
    23221 gatgtttttg ccgattagtt tcttattgtc catgttgccg acttatgaga gtttgggcaa
    23281 gaaggcgatt attcgcttgt tcaatacgat tatgatgcga gcaggagtta ctttggtgat
    23341 tacgactgca tttagcatct ccacgatgtt tttcaatatt tcggcaacct atccattttt
    23401 catggttgct tttctgcaaa ttgtgacctt tgccggtatc tatttcaaat tgggcgacat
    23461 tatgaatatg ttcaatctcc agagtaatga tagccaatca atgggaagac gggtcatgcg
    23521 aaaaccgcaa atgttaatga atcgaacatt gcggcaactc aatcgaaatg tgggacggac
    23581 tttggctttt ggtggtggtg cagcagttgg aaataagttg gcaaaggaac aatcaaaatc
    23641 caaatttaag ccatccggtt cttctctacg aaagaattca cgattaccga acgatcacga
    23701 gataccatct gacttgaata aggaaaagcc aattccaaat aataagaaac aatcccgaat
    23761 gaatttggcc gggagaaaag tcggcaaggt tctggatacc caagctttgg tgaaagataa
    23821 gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc aacttgaaat acaacttcca
    23881 taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt aagcgaggac tggtccaaga
    23941 aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa cgtagagacg aaaaaatagc
    24001 tgaaaagcgc aaagtattga acgaaatagg gaatcctcat gggaaaagaa gggaaaatgt
    24061 tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca ccaaagcgga ttgtgagagc
    24121 aaattcgaat cccgaaatca aacgaaaatt cgctagccaa gaaatcattt caaaaggaat
    24181 aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg aaacggcggt ctactctgtc
    24241 caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct catcccaaac caaggtcagg
    24301 tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt taactttcct aacaatctct
    24361 tttatgggta tcttgatgtg tgtgggctta ttgtttggtg aagacagtga aggaagtagt
    24421 agttcaggtg ctcctcctgg ggggagtagt gtctcagaag aggttttgaa acaccgtgca
    24481 atggtggaaa aatatgccaa agaatcggat ataacagagt atgttccgat tcttttagcg
    24541 attattcaag tggaatctgg tggcacaatg gaagatgtga tgcaaagttc tgagtcagcc
    24601 ggtttaccac cgaattcatt aagtaccgag gcatccatca aacaaggatg tctctatttt
    24661 gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc aagatagtat cattcaggcc
    24721 tataactttg gcggtggtta tttggactat gtggcaaaaa acgggaagaa gcatagtttt
    24781 gctttagctg aatctttttc aaaggaaaaa tcttgcggtc aaaaaatcga ttaccctaat
    24841 cccatcgcga ataaggaaaa tggtggatgg cgctataact atggcaatat gttttattgc
    24901 ttattggtga agcaatacct aactacaaca cagtttgacg ataaaacagt ccaagggatt
    24961 tttgacgagg cgtttaagta tgaaggaacg gcttatgtat ttggtgggtc aagacccgaa
    25021 acaggatttg attgtagtgg cttgactcaa tggtgttatg ccaaagttgg tctgaaactt
    25081 cctcgggtgg ctcaagatca gtatgatgcc atgacccata ttgacctaaa agatgccaaa
    25141 ccaggggatt tggttttctt tcattcgact tatgatgccg gtacttatgt gacccatgtc
    25201 ggtatttatg caggggagaa tcgcatgttt catgcaggag atccagtagg ctggacgaat
    25261 ctaacagaaa gctattggca gcagcatctt atcggtgctg gacggtacaa ataagagaaa
    25321 agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa aggaaaagag cctaaaaaag
    25381 aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa tggtgcttgc cctttggctt
    25441 ttattatctt gtagtcttac ttttggcgtt tataaaaatt tcaccgccat tgatcaacat
    25501 acaactcatg aaaaagtggt tattaaagag aaagtagtga atacttctgg tattgaaaac
    25561 tttacgaagg atttttcgaa agaatatttt tcgtggaaaa ataacaaaga agcaattgaa
    25621 aagagaatga acaacctcga acagtacctt acagaagaag gtttcgcttt aagtcaagat
    25681 atggttcgag cagatattcc cactaattcc gaagttcagt cagtaaaagt cttagatgtt
    25741 gaaaagagct cggaggaatt tgtcgtttca tttttagtgg agcaaaagat tacagaggga
    25801 aaaaaggcac aatcgatttc ttctgcatac cgagtgacga tctttgaaga tgaaaacagg
    25861 aattatatag taacgagttt gccaactatg attgcaaaac ctgatagggc aaaatacaag
    25921 acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa ctacagaaga aatcgctgag
    25981 tttttggaaa cgttctttaa attatatcca tcagcttctg aaaaagaatt ggagtattat
    26041 gttgaaaatg atgcgatgcg accaatcaat attgacttaa agttttcgaa gttagaggat
    26101 ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta caaagataaa ataccttgat
    26161 aatgtatcaa aaaccactag ctactttcag tacaaattaa tattacaaaa aaatggaaac
    26221 tggaaaatca ttgatgttaa ataataatga ctcaatctaa atttatgaga agttataagt
    26281 agtttaactt atttcaggca gaagtcgccc atcctctata ggtgggtgat gaatgccgtt
    26341 cggtaactcg aaaaccgaca attaagacat agtgcctttt tattttaatc catgtcataa
    26401 tcagtctata taagaaatgg ttgatatacc atggaattag atacaaataa tcattcatta
    26461 tttcttcttt gttaccacct tattttagta gtgaaacatt gtaaacatct cattgatgat
    26521 gtaatgttag cttatgcaaa aaactatctt tgaaagaata tcagaatcac atcacattac
    26581 cttagttgag tgaaatcgtg ataaagacta ctttcatatt atgctcaaag cacaacctaa
    26641 aaccgaatta acaaaattca gcaatgccta agctcatgtc tgataaaatg agactttcta
    26701 agggtcaaaa atttcctttg aatacgatca ttagcttaga taaaagtaaa aaccttagaa
    26761 ctacggggat agcttggtta ataagagata tcgctgttaa taaagacata agcttgcaag
    26821 tatgctctat tcccaagaat ctcccacttc aagcactaag gatcgttaaa ttcagctaag
    26881 tggcaagtag ttcactagac gataattatc tccatatttc aatctcagct tttccatttt
    26941 tcatagcaaa ttccgttatt tttatagcat ctaaaagatt cagcttttcc actgatcttt
    27001 caccactttt cagctttttt attgtgctta aattgatacc agtacctttt gatattgaat
    27061 tggaagtaaa atcattaagg gtttttttta ttaaattcat atccgcaatt ccagcgttca
    27121 taagaatgaa gattctcctc tcatagacta ctttgaaaat ggtactacag tggctgttta
    27181 tatatttcag taggttactg tagtggtcat tttattataa ttagaaaagg aacctaaatc
    27241 aaaaatgcta aagaaaattt tcatagtatt aaacctatca tgactaaagc cacggaattc
    27301 ttgataaaag agtatacttg caagcctatg tctttactaa cagcgatatc tcttattaac
    27361 caatctatcc ccgttgttcc taaggttctt gatgttttct aaggcaatgc ttgtatacat
    27421 agcccttgat caaaatattg atactaacgt tgacgtttcg atcatgatgt gtacggcaaa
    27481 taggacaagt ccattctcga attgtgaatg acttcttacc atctgtgtgt ccacattctg
    27541 aaccaatttg actagacgga aatcatttat ctatcttgat gatttttttg acagaagtat
    27601 cgaaaatctt tacataatct gggtgttgca ttcttatgca gatgagagcg ttagctccgt
    27661 attcataacc agttgcctct aaaacgtagt ccatcggtgg atacctagct tgcggttgcc
    27721 agtggctttc gcagtcttct tgtaatcaaa tcgagcatct aattgagtac tagtattttg
    27781 aagataatac ttgagttttt cggttttcag aagttcactt catcttccat catccatgga
    27841 aatcccactc acccatacgc aggcctccaa aaattcttca acaatggttt ttcagacatt
    27901 tttctgaata cctccttgtt ttttgatacg ccagatagtc aggaaaaagg caacaaaaca
    27961 caaaacaaag ctgcctaaaa aggtaatctt cattccatat aaaaagacat ccggccgatc
    28021 tgcgatatag ttagttactc gttggcccat cttagcagac atcgcttgat ataagaccgt
    28081 cgtagaaagc gaaatcccga tgaccatccc caaatttcgg gcaaaggaat tcatggagcc
    28141 tgccagtccc aaattttcga tagtgacaga actcataacc atcgtattat ttggggactg
    28201 gaacaaggca ttgccggcac ccatgatggc agtagcgaca atgtacagcc acaaaggaga
    28261 gtccactgac aaaaacatat acatgacttg ggttaccgac aataaaccca agcctactaa
    28321 tacaaaaatc tccgggccaa cccggtcggt caggtatccg cttaaaggcg ccccgacgac
    28381 catcatcaag ggaaagacca tcatcaacag tccggcatag ctaggactca acccgcgagc
    28441 attttgcaga taaaaaggaa tcactacatt aacaaagaag ttggaagaaa aaatcaacaa
    28501 ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa ataccgaaag aaatcaatgg
    28561 agaggctgct ttcttttcaa cttttaaaaa gctgacaaag gcaatcactg aaattacgaa
    28621 cagtacaagg gggactggat ggaaaaagcc actttcttgc cccaaaaaga ttgttccaaa
    28681 aaggctgata atcgtagccg caatggcagc aaagccccct ccatcgattg tatggccgct
    28741 catggtgata tcttttggta agaatttttc agcaatcaac atcgttacga ttcccaccgg
    28801 tacattgatc caaaaaatgt acgcccaaga aaattgggac aaaatcaagc cgccaagacc
    28861 tggtccagca atcgagccta atgaaacaag cgcaccgatt gctcccaatg cccgaccacg
    28921 ttcatgtatg ggaaagactt ccgtgatgat tccggcattt gtagccatag tcatactagc
    28981 accaatggct tgtaccaccc gtgcgaacaa ttaaatacct agcgaatgat tgaacccaca
    29041 caacagcgag cccaagacaa agatcagcgt accaatccga aatactttga ttttcccaca
    29101 gctatcgcca attttaccaa acagcaacaa acaagaacag acaatcatca gataaacgga
    29161 cactacccat tccgcttgat tcattggtat gcccatatca tctgagatgg taggtagtgc
    29221 aatattgaca atactgccat ccaatgtgga catgaaggta aacatagata tagcaatcaa
    29281 aatccaccag cgattttttt ggacttttgc atcttcttga taactatgca taataggatt
    29341 tcctttctaa tgatcgtgta gctaagaaca atataaccta tgtctactgc ccaacaactt
    29401 ctcgcgactc tagccatagg aagcttgagt aatttcgatt tgttattctc atgtaaccat
    29461 gtaagtttga gatgctttat ttgaacttac tctatagtaa ctagtgataa ttcgctttac
    29521 tttcttctag caatttctaa aagtcgactt cattgttctt cagattaata gtgtgagcta
    29581 tctcgtgaga cgtaagaaaa tttccattct actttttccc aattttttca tatttatggt
    29641 gttgcaccta tttgttttcc aatttgcttt tcaaatacat tgctatgata ttcaattcca
    29701 taggattata ctaaatgtca atagtatgaa taatatatta tagatgttta tatatcactg
    29761 ttaattatta tccacaataa ttaatgatta cctcgatatt tgtcaatcaa aggttgaaac
    29821 aatttatttt cagcatcttt tctaacttga gctgcactct taatagaatc atacgatcct
    29881 aaatagtacc tctttttttt aaagttatat acgcaatcca tttctcttta gaacgataaa
    29941 ctcccttaac accacttgta ttatttttcg gtaattttga cgttagacac tctattactg
    30001 tcccttcaac acatgtcctt gtttcttgcc tttttaaaca tccacaactt tgagtatgtc
    30061 caaaaaataa gttactataa cttatctcac acatactgcc acactcacat tgacaaaccc
    30121 attttgtttt ccatgaacag ttattgtcca aaggatactt tgcagtaagt aatccgaatt
    30181 ttttattttc aatgtgatgc ttttttgatt tcaaacatcc acagcttttt acatcacctt
    30241 ttagaagtcg ctttgccgat actaactttt ttctcccaca cagacattga cattcccaaa
    30301 caacagttcc ttcacttcgc agtttagttg gcttgattgc aactaactta ccaaaagttt
    30361 tatttgtcaa atttttatat ttatgcctat ttttataatt tccacatcca caacttttgg
    30421 ttctacctga tagtaagttt gatcgatata caatcgtttc attcccacag tcacataggt
    30481 aacgatattt tttatatcca ttatgagtaa tctcctcaac aacagtaagg tgaccatatc
    30541 tttccttcac tgcacctacc ctcccaagaa agaagtacca tataatttca attaactttt
    30601 aataaatcag aaaaaaatat tttcaatttt tcttagtctg attttatgac taatttataa
    30661 tacaagactg aacgatacgt acacgaaatc ctcaagatag ataaattgtt ttcatctagt
    30721 aaaacctgct caaaatatga gcataaagac ggcaataata cactcgatat tcgataatgg
    30781 acttgtttta tttgtcaaac tcatcgtaat cgagatatca attccagtct caatatcctg
    30841 atcaaaggtc taagaatcca accattttct tgttactttg tcagattgat tacctttttt
    30901 agtctccgaa taataaggtt ttcggatcaa ctaacatgct ctgagaaaga ctactatctt
    30961 tgcaaatttc caaatgaagg tgtgggccag tactattccc agtacttcct acatagccaa
    31021 ttatttgtcc ttgcaaaaca ttatctccag tttttactag gtattgttgc tggtgagcat
    31081 ataacgcggt tgatccatct tcgtgttgta tcaccacgta atttccccaa gagtagtgaa
    31141 actctgcttt aacaacctta ccagctttaa ctgcatgaat cggctcaccc tgtgtagctg
    31201 ccatatcaag tcctcggtga aattcatttc cacgatttcc aaacaaactg gttattgaat
    31261 aattatgaat aggcacttgg tatccagtct tactcatttg tggaccttta acttctttat
    31321 catagttggt taaatcatag gttttaatta attcatttag cttcaaatag tattgtgtat
    31381 ctgttgcata ccgtcctgta aggaatttag ttgcttctcg atatgtttta ctattagctt
    31441 ttaatgctcc tgcataaaaa tctttatttc cactaattcc cttagttaat aatttggcat
    31501 aatcttcaaa actttcctca tagttttcat acactctaaa ttttgattgg gtcgtataaa
    31561 gcgttccatt acccaaatct tcgtttgtta ccataatgac aaagtttcca ttataagttc
    31621 cttttatccc aaataaattg taattcggtg cctgagctaa ctggctttga ccactggcag
    31681 attctaagat cgcttgtgca atcataacag aagcatataa atcatatttc tgaccaattt
    31741 ttctcgcaga ctcaccaatt ttcataataa aattttctac agattcattt ttgtcaaaat
    31801 gaatttcttc attaccagat agtattggtt gtgattggaa agattctgtt gattctattt
    31861 gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt tggcgcttcc ttttttgaag
    31921 actctatatg gtttggtctt gtcactttcg aagaagttat ttcacttgta ctacttgaag
    31981 ttgttgaaga ttcggcactt gaaatcgtac tactttcact tgacgaagtt gttgaagact
    32041 cggcatttga agtcgtacta ctttcacttg acgaagttgt tgaagactcg gcatttgaag
    32101 tcgtactact ttcacttgat gaagttgttg aagactcagc atttgaagtc gtactacttt
    32161 cacttgataa agttgtcaaa agttcactac tctctgctgt tacatcggaa ttactcattt
    32221 cagtcgtctc ggttctttct tttgcaattt tgtatgttga attttcgctt gccaacacat
    32281 ggaaaggtgt tgctataaag agttgggaca ttaatataac tgctgatgca agttttaaag
    32341 attttttcat ttgatttacc tctct
//
LOCUS       DACCWV010000014        32250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCWV010000014 DACCWV010000000
VERSION     DACCWV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.014"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(279..494)
                     /locus_tag="IVW84_000610"
     CDS             complement(279..494)
                     /locus_tag="IVW84_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916833.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(546..1460)
                     /locus_tag="IVW84_000611"
     CDS             complement(546..1460)
                     /locus_tag="IVW84_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916834.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1460..2359)
                     /locus_tag="IVW84_000612"
     CDS             complement(1460..2359)
                     /locus_tag="IVW84_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP7916835.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2359..2946)
                     /locus_tag="IVW84_000613"
     CDS             complement(2359..2946)
                     /locus_tag="IVW84_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916836.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3110..3301)
                     /locus_tag="IVW84_000614"
     CDS             complement(3110..3301)
                     /locus_tag="IVW84_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916837.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3374..4276)
                     /locus_tag="IVW84_000615"
     CDS             complement(3374..4276)
                     /locus_tag="IVW84_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916838.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4293..4769)
                     /locus_tag="IVW84_000616"
     CDS             complement(4293..4769)
                     /locus_tag="IVW84_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916839.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4785..5384)
                     /locus_tag="IVW84_000617"
     CDS             complement(4785..5384)
                     /locus_tag="IVW84_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7916840.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5388..5441)
                     /locus_tag="IVW84_000618"
                     /pseudo
     CDS             complement(5388..5441)
                     /locus_tag="IVW84_000618"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5943..6422)
                     /locus_tag="IVW84_000619"
     CDS             complement(5943..6422)
                     /locus_tag="IVW84_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916841.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6441..7070)
                     /locus_tag="IVW84_000620"
     CDS             complement(6441..7070)
                     /locus_tag="IVW84_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916842.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7067..8443)
                     /locus_tag="IVW84_000621"
     CDS             complement(7067..8443)
                     /locus_tag="IVW84_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7916843.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8523..9785)
                     /locus_tag="IVW84_000622"
     CDS             complement(8523..9785)
                     /locus_tag="IVW84_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916844.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9798..10145)
                     /locus_tag="IVW84_000623"
     CDS             complement(9798..10145)
                     /locus_tag="IVW84_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916845.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10165..11034)
                     /locus_tag="IVW84_000624"
     CDS             complement(10165..11034)
                     /locus_tag="IVW84_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7916846.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            11355..12782
                     /locus_tag="IVW84_000625"
     CDS             11355..12782
                     /locus_tag="IVW84_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP7916847.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(12799..13605)
                     /locus_tag="IVW84_000626"
     CDS             complement(12799..13605)
                     /locus_tag="IVW84_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916848.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(13693..14493)
                     /locus_tag="IVW84_000627"
     CDS             complement(13693..14493)
                     /locus_tag="IVW84_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916849.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(14832..15941)
                     /locus_tag="IVW84_000628"
     CDS             complement(14832..15941)
                     /locus_tag="IVW84_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7916850.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            complement(15931..16788)
                     /locus_tag="IVW84_000629"
     CDS             complement(15931..16788)
                     /locus_tag="IVW84_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7916851.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(16863..18698)
                     /locus_tag="IVW84_000630"
     CDS             complement(16863..18698)
                     /locus_tag="IVW84_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7916852.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(18747..21137)
                     /locus_tag="IVW84_000631"
     CDS             complement(18747..21137)
                     /locus_tag="IVW84_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP7916853.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(21405..22652)
                     /locus_tag="IVW84_000632"
     CDS             complement(21405..22652)
                     /locus_tag="IVW84_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916854.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            23044..23460
                     /locus_tag="IVW84_000633"
     CDS             23044..23460
                     /locus_tag="IVW84_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916855.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(23477..24214)
                     /locus_tag="IVW84_000634"
     CDS             complement(23477..24214)
                     /locus_tag="IVW84_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916856.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(24636..25199)
                     /locus_tag="IVW84_000635"
     CDS             complement(24636..25199)
                     /locus_tag="IVW84_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916857.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(25186..25713)
                     /locus_tag="IVW84_000636"
     CDS             complement(25186..25713)
                     /locus_tag="IVW84_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916858.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(25736..27073)
                     /locus_tag="IVW84_000637"
     CDS             complement(25736..27073)
                     /locus_tag="IVW84_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7916859.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            27193..27672
                     /locus_tag="IVW84_000638"
     CDS             27193..27672
                     /locus_tag="IVW84_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916860.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(27787..28019)
                     /locus_tag="IVW84_000639"
                     /pseudo
     CDS             complement(27787..28019)
                     /locus_tag="IVW84_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28022..28339)
                     /locus_tag="IVW84_000640"
     CDS             complement(28022..28339)
                     /locus_tag="IVW84_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916861.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(28450..28632)
                     /locus_tag="IVW84_000641"
     CDS             complement(28450..28632)
                     /locus_tag="IVW84_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916862.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(28669..29469)
                     /locus_tag="IVW84_000642"
     CDS             complement(28669..29469)
                     /locus_tag="IVW84_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916863.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(29606..30025)
                     /locus_tag="IVW84_000643"
                     /pseudo
     CDS             complement(29606..30025)
                     /locus_tag="IVW84_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30021..30329)
                     /locus_tag="IVW84_000644"
     CDS             complement(30021..30329)
                     /locus_tag="IVW84_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916864.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            complement(30491..31021)
                     /locus_tag="IVW84_000645"
     CDS             complement(30491..31021)
                     /locus_tag="IVW84_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916865.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(31037..31402)
                     /locus_tag="IVW84_000646"
     CDS             complement(31037..31402)
                     /locus_tag="IVW84_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916866.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            complement(31408..32151)
                     /locus_tag="IVW84_000647"
     CDS             complement(31408..32151)
                     /locus_tag="IVW84_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916867.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
BASE COUNT         9216 a         6456 c         4472 g        12106 t
ORIGIN      
        1 catctttaaa gaaatacgat caaaaaacat aattcccata ttatttgctc gttcaacaat
       61 ttgaaatata tctattagtt cttctttttt cataactgct cttcctttaa cttgttaatc
      121 taacttgctt acaaatatat cctaccacat ttgtgtacta tctgtcaata tttttttgtg
      181 tataaatgtg tattataaaa caaataattt tacacaaaca aaaaagagcc taactgctct
      241 tagcaataag ctctcatcac tgctcctcgt gaatttagtc agtacaatgt atttgccttt
      301 tctttgataa aatccttata cgaaaaattg ttcaaccaaa attgttttag cactaccgaa
      361 tttttgtttc catcattcaa ctccaattga ccaaatctgc tgatcatatc atcaatttca
      421 tcaattatct cttgtgccat catgccgtct tctagacgtt ctaatacgta caattcaaaa
      481 acatcccttt tcattcaaaa tgcccctttc aggttacctt ttgccatttt atgggctaac
      541 tccgcctact cagctttttt tatataccta ctgatatttg ctttcgtatt tcttggcata
      601 ccacctgctt gcaggtaatg ttcacgataa taagcatcta gctctgaaac agctttttct
      661 aatctattcg ttgcattcaa cactgagcgg tattcacgtt tttcccttaa tacacgtctt
      721 aaatggttca tcgctgatat ataaggctta tagtattcca atacagtata tcctctagaa
      781 atataggcaa ttggatttct agcttcatgg atcaattcta aaatatctgt tggatcaggc
      841 ttatccggca tctcttcttc gatttccata atactgatca agtagtccaa tccggaatca
      901 tcgactccat aactttcaca ccattctttt aggttgttca agtcatgaat aacatcttca
      961 ggatcaaact ctctaaaagc agtgatccct tggctataat attttatata ttccattgcc
     1021 gattcaaaat cctctggcgg tatgaaagct tcaatttctt cttcatcaat cgctttcagc
     1081 actattccga tacctgagtt tgaaggatat tttctcaatc cttcttcaat taaatcctca
     1141 tctattcgtc ctttttgaaa catcaccaat aattgttcta acagctgatc taaaaccctg
     1201 cgctcttcat tcagttccat tgcattttta acttcttttt caccgagttc aaagtaagct
     1261 ttgatcaact tttcttgttc ttttcgttca actaatttct tcttcaagtc attcatattg
     1321 agaatgaaat ccatgtgatt actcttttgc tgattgacaa aatcatatct ttgttccaaa
     1381 acatcccgat atttttttag ttcttcttga attatttcaa agctatcaag tacatgaaga
     1441 attttttcat tattattcat cagcccatct cctgtagtct cacgtttgtt ttcgactttt
     1501 caaaagtatt gtatttaaaa gcaattaacc ctacaaccct gctatctttg atgcctattt
     1561 ccttgttaaa aattccgtcc aatgttcctt tgactaaatt atcaatatcc ggctgtgaag
     1621 tacgcatcag tgaatcaagc tttttcttat cttttctcac ttggtaaaaa taatctatct
     1681 gagctataac ccccatagga gcattcatta cctttttcag ttgatcattt accaaatctt
     1741 tctctttaag cgtattcttg atattttcca aatagtcgtt gtactctttt ggatagtaag
     1801 tgataatccc atttcctcta aatttagact ttggtcttgg cgttgctact ggtttaaaat
     1861 caaatgtagc ttcccatata tctccgttca atccttcaga agtatcccac tcaaattctg
     1921 aatatcgttt gatcctcaca actgtttttc cttcgttcgc cacattatat ttaaaagcaa
     1981 ttaagcctgt gatatggcta tctcgtatag ctgagatgtt aaaaatacca tccatagcac
     2041 tttttactaa attatctaaa tctggcgctg ttgtcttcat aatgttatag acttttttat
     2101 agctttttgg tatctggtag taaaagtcca cttccataat tgcgcccata ggatcattta
     2161 caatcgagta aaattcatca tctaataaat cttgatcttc gataaagctc tttatttttt
     2221 tcaaataatc aagatacttt ttatcatagt aggttgaaat ccttttctct gctacgtcat
     2281 tagtttcatc agcagcattc cttcttgcac ttggtcttgg tgcagataca ggttggaacg
     2341 gtaaagttac agtccacatt agtctttttt gatctctttc aaatagattt ttttaaaggt
     2401 atcaaggctt aaaggagcta ccttttgcat cttccggatt ttatttactt gttccaattg
     2461 tttcaataga ccatcttctt ttctaaggta ctcaggattt gttatttgtt tattccgata
     2521 ctcgattaat gattgaagct ctcttactgt taaaacatcc tcaatttttg cttctttact
     2581 cgttatcttt tttagatacc ctaatactgt tggataagat ttgccgatca cttctgcgat
     2641 ttctttttta ttgtactcac caaattttcc aagcaaccag tcattcatga tcttacttga
     2701 tacttttggg tatacaagaa ttgctttttc aatggaatga ctgccattat ttctgatcag
     2761 ttgactatct ttacactcaa ctgtaacgcc atcacctaaa tcaacaacat attgccaaac
     2821 gcctcttcga ttcttattaa gagaaataat cgttccctct ttatctctat aatcctttga
     2881 taattcactt actgttgtaa ctctttcccc aatgctaaaa tgtctaatat cattattgtt
     2941 ttccacttat tatccctcca atattgctgg ttatctcttc ctctgacaag gaactatata
     3001 tcatataaag aattttaaca ttattcggaa tgagtgtaaa gaaactttat ataaaaagag
     3061 tgatttagtc taagaccaaa tcactctttc ttttttatta attatattgt tatctgatag
     3121 acctattagt aaatgcacgt cttattgttt gatcattctc gatcaattct tctaacaact
     3181 ctgaaactgt tactttctta cctcttgctc tttcttcttc tttagctcgc ttttctaaat
     3241 atttaacaga agctattttt aaagtcacat actttctaac tttttcttta tttactacca
     3301 tcagctatca tctccaaggt gtcacaccta tatattattg gtgtgacacc atttgtttat
     3361 tataactttc tggctattta gccaatttaa tcgttaatgc ttcgtttgct gtagaatctc
     3421 caaaaacttt accgtcttca taaactacat gaactggcaa atctgcaccg tctgaatctg
     3481 gtttagcagt accacgatac aaaccaatta ctctttttcc tagttctctt gcaaccgtaa
     3541 cttttttatc ctgatccata gattcaactg aattgaataa ggtaacatta atcatttgct
     3601 tttcagtatc caatttaaca gttttgatag ctttgaagac ttcagtgtta aaccgatcag
     3661 ccatagaacc agaaacagat tcaaacgatt tgactttttc aggatcaaaa tcaattttat
     3721 ctggtagttc cgcttcgaca gtagaacttg attcagttgt actttcttct gtagctactt
     3781 cttttttcgc tgattcgata atctttttcg ttgatgtttt atttattttt acatctttat
     3841 agtattcttc tccatcaacg ttaaaaacca atgtcagctt gtgtacaggg ctattctctt
     3901 taactgtata aaacacttca aattgtgatt gagacggctt aatagtagcg agaacgtcgt
     3961 ctgatgcttt cttgccatct tccttcactg tcagcgaaac atttttataa ctcgttacat
     4021 tttcaaatgt tccatctatt actacccttc ccaaagaatc tggagcataa ctattattaa
     4081 ctcccgatat accaaattgt ttatcgttac ttccaaaaat cttagtggca ttaaacacaa
     4141 taaaggttac aatcacaatt ccaatgccta tgttacgcat aatcggatta tttgtttgaa
     4201 attgaccaat aattatagcg attgccccaa aaaaagcaag aattaaacat aaataaccta
     4261 acagggttaa tagcatgatt catcacaact ttctattctt tatttttctt tttggttttc
     4321 gtttgttcaa actcatgctc atttagataa gtaatcaata aatctcgcat ggatttgcta
     4381 tccttactta gcgtatcaaa cgcttcttgt aagtcagcat ccttctctac agcattaaac
     4441 caatgcttcg ttccaaattc aaaaagatta ctaatcactc ttaacggttc ttgatcagta
     4501 ttcgtattaa gccattcaag tatccgttgt tgagctaatc ctaatttact ttcgtcttcg
     4561 tgatttgtac gtgcttcata gattttagtg tgtatatcat caaaatagtg gattcgttct
     4621 cttagcgctt cacgttgttc gtacatagcc cttttatttc taacttcttc gtctattgtt
     4681 tcaatctttt ttcttaacag tatttgaagt gataacattt ctgagacaga caaaaacact
     4741 ggaattttct tttcttttct ttttggcatt tttagcactc ccttttaatt cttttgtttg
     4801 atcaatgaaa cgttctttag aataaatccc aagatcattc cacagacaaa tccgattaca
     4861 tgtgacaaga catttgcctc gcctgattca aaagtagata ccaataaaat gccgattatc
     4921 aataatacaa aaacacttag taataaatgt ttcttgctat caaaacggat gtatgcaatc
     4981 gcaaataaag caatccatcc aaaaatacct ccactaagcc ccatacttac gatgttgctg
     5041 tgtagcagaa aaggaactaa gcttcctaga aatcctgata tgattaaaac gatcaataat
     5101 gttaaccact catactgatc attcagtgct tcttcaagaa ctactaagaa caataaatta
     5161 gaaattaaat ggtacaagtt aatatgagaa acgatagaaa tgaatatatt cgggaacaag
     5221 tgtccttcat acaaaccacc taaagcaacc atttcctttg cactcaatgt atttccaatt
     5281 atgctttttc caatctgcaa aaaatcaaac aggaaaatca atacacaaac aaaaactaca
     5341 gtcgctacta ggtatcgact actatttttg tttttttctc tcattcgcta cttcctttgc
     5401 tttagtaact gccgattctc catattctct aaaaagagtc gatgttagct ttcctctcgg
     5461 aatattgtat ttttttaaaa tttcagttgt tgaaagattt ttttcaaagt cttcaataat
     5521 ttgtttatga agatttattt tagccaaatc attggttgtc aaaacaggtt ctttagcttg
     5581 aatatcgcct tgacgaatta acctttgaat tttatttttg ctttttaaaa aaggatattt
     5641 ttcaattaaa gggtcaattg cattcccttc atcaatccac ttacctattt cttgataaac
     5701 tctcttttga aattttttcc tcagctcaat cgggttcgga aaaacattgt gcttaaaatc
     5761 gtgtgatact aggctctttg atttgcctaa ttcatcagcg acttcttgca ttgtaatttt
     5821 gttttcgata tagcattgga caagagcatt atatttttta gggttattat tatttattgt
     5881 catgttctta cctacgttca ttgctaattt tttggtattt ttctgtttgt gtattgcgat
     5941 ttttaaaagg catttcctaa tacgttaaat tttgataatc ggttcaattt tactgcttta
     6001 tcaatttctg cctgtgacca aggttctatt aaacgcattt ggtacgtcac tagcagatcg
     6061 acatgttcat attcaaactt ttctattccg taaatctgat aaaatatttt ttcttcgttg
     6121 ttactataag gaatcactgt tccgatctta aatatgtttc gtgtgttgtt tacaccaatt
     6181 tcatcagtaa tcttgtgtct atattctaat aacacgtatt tcttatacct gcttgtcaga
     6241 tcaggtgaat agttaacaga ttgacaaacc aagtcactgg ttatacgtgg atcacctatt
     6301 ctgttaattc tcgtgttata gattccgata ataatatagt ttttatcttt atattctata
     6361 gtttctccca cttctaactt ttgattaaag cctgttctat ttgcttcaaa aagttctagc
     6421 aatctttttc acctctactg ctactgccaa acaaatttag ctttatcatg ccatgtttca
     6481 gacattactt ttttaccatt ttcgtcaaat atcaccgctt cccactttgc tgttccatcc
     6541 ggctgtttac aaatatctgc aaaccatagg atcgtttttc gatcgttgaa tttccttact
     6601 ttcttctcaa atttattgat ctgaactcta ttttgattgc tgttccaacc tactaatagg
     6661 cggttatctg attctgtacc atgaaaacca atgggaacga tgtgggttct atcaaatgct
     6721 tcttttcctt ctggatagag cattggtcta agtttcctac gtttcaagtc atctcttgct
     6781 tgataccgcc atgccgattt tggattgaat ttaatagcta tttgatgctt gcttttgtca
     6841 tggtaaaaat atgcgacatc ggcttcagct ccaagcaact tttctcttgc actatttgat
     6901 tcaaacggtt tattcacttc taaaaccttc acccctaaaa tgccatgatc aagcatctgt
     6961 ttaggagtta gtaccgcttt tttatctctt ttcttcgctc gtttcacaac tacttcatct
     7021 gtatatttat atggagtaag ccatcttaaa aaagaactga cgatgatcat ttatgcacct
     7081 gtttttttgc ttttaacatt ttttgcatga taggtgaagt acttccattt atttgtttct
     7141 tttcagattt atcagttgtg atcactacgt tttctgagcc ttttggatca ttagctttag
     7201 gtttggtttt agatgcaatc ggctgtttat tattcgtgtt gtttgaggtt tgcggttcag
     7261 gatcagcttt tcctaatgcc cttaaaactt tttctagttt ttctgcttgc agtttttcta
     7321 gtgttttagc tccctgctcg actttttctt ctttgttctt ttcaagctta atagcaaacg
     7381 cactcttctt ttcaattgat ggctctttag atgatggatt tgctaaaggt gtgttcaaat
     7441 ttcttttagc agattttctt acaggagctt taccaattcc accaaaatcc tcgtcttcac
     7501 cgccctcacc acgagtttca gctacgattg ctcggtaatc aacatattct ggttctggca
     7561 tagtatttct aaggtgattg aagattgttt caatttcttt gttactgatg taagctccct
     7621 gcgctctaac catacttcca tcaacaagct taataagcat atctcccttt ggacttagac
     7681 tttctccacc gccttcatct aatacaatcc tactttctaa atcacttgct accataaggc
     7741 aaacacgtga cggaaaattg gctttgataa taccagtgac aactgttgat cgaggagatt
     7801 gtgttgctaa gataacatga atacctgctg cccttgccat ttgtcctaat tgaacaatca
     7861 aaccttctac ttctttatgt gttccaacca actgtgaata ttcatcaact aataagatca
     7921 catagggaaa tctatctaat ccattttttt cggcatattc gttatatagc gtgatgtttt
     7981 gtaaccctgc tttttccatc agtttgtaac gttcatgcat caagatcaca agatattcaa
     8041 aaaaatcata agcttcatcc atatctgtaa ttggatcagc caacatgaac ggcaattttt
     8101 tgtatcttcc aaattcaacc ctttttgggt ctacaattcc tatttttaat tcatcaggcg
     8161 ttgagtgaca cattactgta acaagaattt cattcagtaa tactgatttc cctgatcctg
     8221 tcgttcctgc aaccaacatg tgtggcattt cgctgatgca aaaatgagca attgtgccat
     8281 caggcttaat tccaaaaata gcatctgttg ggtctttagc ctttgtgttc aacatattga
     8341 cactgaattt tccattaagc tttatattag gttctttatc tagcaaaata gattcagctt
     8401 tttcggctct ctttttgact ttctctaata atttcatagc catttctatt cctcactttt
     8461 ctcggtactt ttctgtttgt gaactggtag tttgtatttt ctgtttctat ttgtgatgtc
     8521 ttctaaaaca cgctattgat cattgatggt acatcaattg gaatacgatc attttctcct
     8581 aatggtaaag taatttgaat acgccctgcg ttgatccgca cgattacacc tgatatatgc
     8641 aagtaagttt caatttcttc tttcattgag cttagttgaa tttttctggt acgagataaa
     8701 gtaaacaaat attcaataga tgtattccca acgtgcatat ctgttagact tgcaggtacg
     8761 tcagcagaag atagataaag ttctaaagat tgcttttgag tttcggcata ttttctagct
     8821 ttttcggttt ggttttggat atttttggaa ttatcggcaa agagattgat tggaaaagaa
     8881 aattgtgaat ttgaaactgc gattgaagat gttgctcttt ccgtttcttt gtttttggta
     8941 cttactcttt ccttacgttc tctttcaata tttactgatg ctcggaaaat gaaataatcg
     9001 cctgaaactt catcttcttt ttcaccaaat ttggctcgtc cgcttgttgc tcttgtcagg
     9061 atcgacccta aatcttgaat ttgtcgtaaa actgtttctc taactttctt cccttttggt
     9121 tgcgggattt ttacgatata ttctcgaata tcttcattga cacttagatc acgtcttgta
     9181 tgaacagaaa ttttgatatt tttaagcgtt gatagcacat cggattctaa ttgcgttttg
     9241 tttcgttttc tttttttgtt gttcgtttca tctcctataa tcttgtttgg atcatacttc
     9301 acatccatag cttctagagc ttctcttttc aaccttctac tttccatatc atcaaaaaat
     9361 ggtctttgtt ctccatagtg atgcctgaac atcaaagtaa ccaaatatcc aacaaagaat
     9421 accgttcctg cttctaatac taaaatagag atgtctacca agtgtagctt tgcaaaaaag
     9481 ctttgtacat tatatgaata acttaaataa ttgggaaaaa acgcattgag taactcttta
     9541 aacatctctt ttaatcctaa atcatctgtt ccgttaacat catttaacga gacaagaata
     9601 cttactaagg tatctacaag cagttgtaca acctttgtta cccaatgcaa aatatatatg
     9661 ccaattgcac caactaccaa tgcacgtcca accagatata ctgatttaga aaaactggct
     9721 acaggataat atttttctcc atcgcttcgc ttatattcct ttaattcaat ggatttttcg
     9781 ctcatctgaa tatctcctta ttttctttct gattttggtt catcgtcact tatgctgtcc
     9841 tcacacatct gcgtatcttt aggaatgatt actctgattt cgtctaacat ttctctctca
     9901 atgatcatcg ccccttttgt actaacagtt ttctctgata attcaacaca tttccctatt
     9961 tcagaatctg gatcaaaaat agcctgttct acgtcaaaat gattcttagc tacatacacg
    10021 atactcattg tttctaactc tctagcaact gaatatttac ccttcttcag aagatagtag
    10081 ccatttcctt tttttattcg atttgcaact gatttatcta atccattgat gcttattgtt
    10141 tccaattctt caccttctca atttctaatc tcttacccaa gtaccatttt gatagttgat
    10201 gttgattcct ttttgattgt taaatacata aacattaagg tttaccgttt ttccattatc
    10261 gttaactgag aaagcttcga catgagaacc acgaggaact aattcattcc ctttataaat
    10321 tggatcaact ttatacaata ctttttcatt cttattttta tagattgcat cacgtacaat
    10381 cgtttctgag taagccatcc ctccattctg atcatttttc ccaacatttt gcgctctcgt
    10441 tccaagtgtt aaattgtgta cctccatatc tcctcctagc gaccatgcta atgaatgact
    10501 tttgttatag agataacctt tatagccttc tagctgagtg atttcattgt ttgaatcttt
    10561 gccgacccaa gtatctctct cttgatcaaa tactccatcc ttgtattctc ctgcaatatg
    10621 gacagaactt ttgaaagatg gacgttccac atttttagat gagtgagaat ttacagcatc
    10681 ataggtgatc aacgcttcaa catctcctgc tcttccaagt tgatctaaat ctgcataatt
    10741 tacccaaaag gtttgactgc ctttttctga taatgcttca tggtcttctt ttgtcagtgt
    10801 atatttccca tcattaatgt ttacgatatt atttggatat ttgtttggat cataacgtat
    10861 ccctaaaaat tcttttgatt cactgtctgc tttattacct acctgaacac tacttttgat
    10921 ctgaaaatca tgttcctgag tactgttatt ctgaaagtaa cccttgccac taagaccaac
    10981 cgcaactaaa accaaaattc ctaacaacga tcggctaaac ttcttctttt tcataccacc
    11041 attcctttta ttgtgttatc ataataagat aaatatataa taaactatat tgtaaatttt
    11101 gtcaaatgta tttttattta ttcccactat aaaccttgat atatagccaa tttaaaaaag
    11161 gatggcaaaa aattatcaag ttaaaaaaat aaattttgaa ccaaattatt tttaataaaa
    11221 tagctagttt ttttatcaaa aaaaatagag cttttcagct ctattcagac tgtagacaaa
    11281 ctcctaaatt tggagtgttg tttgcagttt tttgttttat actaattgta taaactattc
    11341 tgggagtgat tctaatgatg agaaaacaat cgattgaagg tagaaatcaa tttgctatgt
    11401 taacaattga tgatcttgta cctaaagatc acttagttcg aaaaattgat gctgcgattc
    11461 agtttgattt catttatccc attgtagaat ctacgtattc aacctatggt cgtcccagta
    11521 ttgatccagt ggtcttaatt aaattagtat ttatccaata tttatttggt atccgttcga
    11581 tgagacagac aattaaagac gttgaaacaa atgttgctta ccgttggttt ttaggttatt
    11641 cattcgagga tcctatccca cacttctcta cttttggtaa aaactatgtt cgaagattta
    11701 gagaaacaac tttatttgaa gatattttct ctcatattct agaacaagct gtaaaagctg
    11761 gcttcgttac ggaagacaac ctttatattg attctactca tatcaaagca aatgccaata
    11821 aacataagtt cacgaaagag atgacctacg gtcaagcaaa agcttatcaa gatgaattag
    11881 aagatgaaat aaataaagaa cgtattgctg taggaaaacg tccttttaca tgggacacag
    11941 aaagtgagtt gagtcttcaa aaaataagtc attcagatcc agaaagtggt tactatgtta
    12001 aaggtgaacg agaaaaacaa tttgcttatt ctgcacatac ttcctgtgaa gataacggtt
    12061 ttatcttatc tacattaatc acaccaggaa atgtgcatga cagccaagtc gcaatcgatc
    12121 tggttaaaca gtcgaaaaga gcttttccaa atataaagcg tgtggttgct gatgcaggat
    12181 ataagacgcc taaatttgtt cattttctgt cacacttgaa actctacccg attcttccat
    12241 atactgcacc tcatggagtg aaaggaatgt ttcgtaaaaa agactttgtt tatgatgcct
    12301 attttgactg ctatttatgt cccaataatc aaccgttgtt attttcaact atcactcgtg
    12361 atggttatcg aacctataaa tcaaatccaa aattctgtgt gagttgtcca ttacttgaaa
    12421 attgtacttt gagtacaaaa catcaaaaaa tagtgactcg acatatttgg gctgatctta
    12481 tggatgaact tgaacaccaa cgccatacgg cgttgaatag agaaatttac aaaaagagaa
    12541 aacaaaccat tgaacgaata tttgcagatg caaaagaaaa gcatggtatg cgttggacga
    12601 aatatcgagg tcttgaaaaa gtcgccacgc acacgatgct tacgtttgct gccatgaatt
    12661 taaaaaaact agccacatgg ctatggaaaa caaaaaagcc tatggctttt tatttcaaaa
    12721 ttatgcaaag actaacaaaa aggagtggct taattaccaa ctcctttttg tctacaatct
    12781 gaatagagct tttcagctct attcaatatc attttgatca attaataatc gtacactcca
    12841 attaaccgag ttttgatcac tcaaatattt ataatctttg tcatcaaaaa ccagttgcca
    12901 aactctggta cgaacagttt cttcgtccac ttcctcatct gaagagtatt gcgataatgc
    12961 ttcttcaact atatacttta caacttcctc aaaatcagta ggaactttat caataaccac
    13021 ttcatagtct tcttgtttta cgatattggg atcaatatgt tcatccaaac atacctcagt
    13081 gagtttttct ttcacttctg tatctctttc tgtttttagg tactctgcat agtcttcctt
    13141 agaccattgt ttttcaagat catccgctat acttttaata aaggattctt tatttaacca
    13201 attatctgcg gtatctatac cttcaaactc aaaagctgtt ccttctatac gtgatttgac
    13261 gaaagcgtca attttcttgt attgattatt catttcccat gaaacacact catcttcttg
    13321 cgtaatatca taaatttttt cggaaaaact aactaattca ttccacgatt tatcctcggc
    13381 aacttgactt agtttcgatg caataaaagg aaattttgct tttaagtcat ccatattctt
    13441 gatctcaaat tcgatatttt cttctcctac agctgaaatc catgctgaaa gattgattgc
    13501 aatatcgcta aagtcttcta cattttcgac atttcctgta ttatctttta ttactgcttc
    13561 taaagccata tcggttgttg cactcatggt ttgatctcct ctcataatag agcaaaaagc
    13621 cggtttaacc gactttttgc atggtacgtt ttgtttggta gttttctgct tgtgtttatg
    13681 ttgaaataat ccctatagct cttcttttaa tagttcttca actttatctc ttaactctcc
    13741 ctgaccactc tctaatagat ttaaatagct catcagaata tccgtttcat acgtttttaa
    13801 ttcagctcta tactcacttt ctgaatatga tcgtaaatac ccatcattgc taaatgcaac
    13861 tagatcatct tgaaagttcc attctcctaa atttgtttta cttactgctt cccatggact
    13921 attactaaac atttcattaa agaaatcttc gtcattttga tataccttca attcaggaaa
    13981 atagttgctg tcttcattga tttcttgcac catttttcca agttcttctt cagataattt
    14041 accttccaat aattttactt tttctctgtc atttgcataa gccatttgaa cctctccttt
    14101 ttctttttca atttcttcag gtgtttgttt tggcgtaact ttcaaatcta ttccattgcc
    14161 tattagacct attagataac catagaacgt tttaaagtca cttgtagcaa gagatttggc
    14221 atcagggtat gtatcccctc caattttatc taaaacaaaa tcagcaatcg ttacccaagg
    14281 agaatatatc gtacatacta catcccatgt agcatcttca atattcgtcc atgtcggaaa
    14341 gtcattttca ttcaaatagt cttttttcat tgtttcgatc agagtttttc cactttcgtt
    14401 ttggcttaga tattcaacaa attctgcacc atcaaacctg ctactaaata gtttagctaa
    14461 tgagtctaaa gctgtttttg atactcccac cattactaca cctcctatta atattttacg
    14521 atgacttgtt tttcattcac aaataagatt ttctacatct tatctattgc tttatagctt
    14581 ttccatacat tatttctaaa tacaaccaat aaccctatta aattaagctt ttgagtttat
    14641 cttttctaat aatattctga taaatattct gataaaaaaa tttattgtat ttttttcttg
    14701 actttttctt tagaattaag atcagttttg attttattta aaagtgaatc tagctctatt
    14761 caaaataaaa aagagatcaa taatagtatc aatccctttt tactgtactc tatcaatttt
    14821 tataatcttc attatgcttt tcgaaactct ttgattaaaa gaataagaac ctttagactt
    14881 gctaatgctc ctattacagt ccaaatgact aaaagcattt tctgatgttt attggtaatt
    14941 cctgtaaata ttgaacttgc cttttcagca aacgttctgt cttgcatctc tacgctacct
    15001 tgttccggaa tattaagatc attatttcca tctaattcct tgttggcacg atcaacatct
    15061 tcaaataaat cattagaaga tgtaacagtt tctgtcttat ctgaaacgga ctcgctatca
    15121 atggcatcag cgtatgcggt attgccaact gttacttgtg gcgtgatgta ttttgctttt
    15181 tcatctctta gacttctaac taataaacga tcgtagttct gaggatatgg atgacaagta
    15241 taaagtgtta acatatctaa atcaggaata tcagtcagtg ttgaattctg accgtaattt
    15301 ctaataacgt ctgttcggaa aaccgtataa actgcttttt caccaagata atctatataa
    15361 atgtggtctc cggctactaa ttgatcaaca taaagaaatt gtggtgacgt ataaccgcca
    15421 cgatgacctg ctattactgt ttgatgacct ttacctgata aaggtagact agtagcactc
    15481 atttgtgcca cacctctata taaatgctcc tgtgaagcgc ctagaaacaa aagctctagc
    15541 acatttattt ttggaatata gacataacca accgctcctg aataggtaga tggttcaaaa
    15601 tctttcgttt gattttttga aaaaacatct cctgtgtatg gattgacctt tccttcagct
    15661 tcacgaatat caaatgatcc accgtctgtt tcctgagtat tgtctactcc tttatctgtt
    15721 ttctgagtat tgtctaatcc ttggttaccg ctgttcgatt ttatttgttc gacttttttt
    15781 gccacttcct cttttgaagg agtttgtttt ttctcaattt tctcttggat ttccgccaca
    15841 ttctcttttg ttttgaattg atgatagagc tgatttgcag gataaattgt taaaaccaaa
    15901 gtgcaaataa gagtcaaaaa aagtagtatt ttattcttca tttttcattc ctcgtctctt
    15961 tgctactatt tttttaataa tgactatcac agctattatt aataaaaccc ctacaactac
    16021 gataggatac cagttcatct gcgcctttac ctttaaatct tctttcttct tttctttctc
    16081 aacaatactt ttttggtaag gtatgctaac ccctctaacg agtaacctat gagaattatc
    16141 tccaacaggt gtacatgtca taagtgttac ataatctttt cctgccacag gaagcaaatc
    16201 ctcaaattcg gttggtagaa ttgtttttat ccgatctact tgataagcaa gttttttccc
    16261 tgaaatatca ataaaaaatt tatcaccaag tttgagcaac tctaaatctg taaaatattt
    16321 tttattagga atgcctgaat gagctgatat aacagaatgg tttccaatcc caccagttgg
    16381 ataatcagtt ccggttaata ttcctgctcc tgctgatagt tggaaatcgt ttacaccatc
    16441 ataaattggc aaatccacat taatctttgg tataagaatt tcacccacta attcacctaa
    16501 tttatccttc tgaagatttc catttgacgg aatattgtta ttatcttcag gaatcgtgac
    16561 tgaggtttgt tctgtgtctt ttcgtttcct attaacttct gaatatctct tgaagacttc
    16621 ttcattctcc ttttcagaag cttcatcata tttttcctta taggagttta gtgtttgttg
    16681 agtttggtag taggtataag cctgagtaat tacaggatat agacctactc ctaaacatag
    16741 caacatccca atttttaaga tgatcaactt gatattcttc tttttcatgc tcatacctac
    16801 ttttcacaca ctttttaaaa agaaacccta gagccaaaaa cactctaggg aattttattt
    16861 cattaagctt tgtgttgttc tcgtttgtag acaacatata gccctgttcc tgccacacct
    16921 gcaaccacta gaaatactac tgttccaatt ccacctgtac gaactgtttc aggtgtttta
    16981 aggttcttga tgtctgttac ttcgctagaa gcatcatcat tcgataccac aaatggaaca
    17041 tcatttctag ttggtgtata tcctgaagga gcaacaactt cttcaagagc ataatcattg
    17101 atttttttat ctgtgattgt tggattacct tgtttatcaa aatctacatc ataggtatat
    17161 tgcaagcctt caatttggaa cttaccatct gatccggaaa cgaattcagt agctttagat
    17221 ttatctgcaa tccaagtaac agatttcgtt gttggatctt gttttaggta agtaacttta
    17281 ttcggatcaa ttttgttctt cactacaaat tttgctccac tcaatgcttt gcttgtatct
    17341 ccatctgttt taatgaattg gtgatcacca atgattgtat aagcgtgatc aactggtgtt
    17401 tcgtcttcgg gttcatatcc attgttgtag atcgttgaaa catcattctt aagtggtgta
    17461 tccattttag ctttactatt agtatgagcg ttcatcaaaa ttgtaaagaa gcgatcccct
    17521 gcatcaaatt gtttctgaag cttatccata cctgattcag taaagacata gtttgtcgtt
    17581 tgtccgtcac gttcgatact gtaatcagta tctaaagtca ttccttcgat cacttctgat
    17641 gaatcataag ttaactctgc acctagaaca tctttgacaa catatttttc gtaattacca
    17701 atattaccag gaattgatgg tttcaatgta tattgaacat cttcgccagt atcaacggtt
    17761 tctttatcaa ttaccttagt aattacaggt cgtttgtagt tggtaacatc aagaactact
    17821 ttagcagtct ccccacgttt aacgctaaat ttgaattttt taccatctag taaataggtt
    17881 gttttattag catctgttcc caaatcggtt tctaggaaat aatagtcacc taattccaaa
    17941 ccatctacag ttaatttacc tgtgctgtct gttttataag ttccaacaac ctgatcagat
    18001 ccatcatctt ttcgcaattc aaaaacagtt ccggcaagag cctttccatc ttcgtctttt
    18061 ttatcaagct ctgctgagtt taattggaaa tagtttttag gatagacatg tacatcataa
    18121 ttaaagtatt gatcatctga tccgtttagt tcaggtacgt taacaatcat aggatacgct
    18181 tcttcaacta cacctttgac tgttcctgtt tcaacaatca aatacaagtt atctgttaaa
    18241 tcgtctgtat ttacttgtat aggtatgtct atattccctt gtgaatcagt cttcattcta
    18301 cttacttcac tgtatttttt gtttagtaat aatcgtgctt tagcatcatc aatatgttga
    18361 ccttttgtag tatcactatc cgtccatccc aaggatttag cttcatcttt agtaagtttc
    18421 acataagaga actcaacgcc ttcaagaggt tttacaccat ttggtaaagc tatttctttc
    18481 cctttgttat caatatctga gacagggttc ccatccccat tagtatttgt tactttatgg
    18541 atatgaatcg ttccagttcc ttgataatta tagttttgtt gtgcatatgc tacttgcccc
    18601 cctagtccaa ggctagaaga gattgagctt gcgcccaatc ccactcctag caaagtacca
    18661 aatagcatta aatttgaaaa aatttttttt gatttcatac gtcagccttc ttttttcata
    18721 tttacttctt ttccattaac acttttttat tttgctttgt gctgttctcg ataatacagt
    18781 agcaccatac tcaatcctgc aattagaaat acaagaagga aggctgatgt accgattccg
    18841 cctgttctta ctgtttcagg tacaaggtga ttaattactg tatattcaaa ggtttgtcca
    18901 tcgtcagcaa cggtgatgcc ttctttagac catttccctg tataaccatt agggttttgt
    18961 gtttcttcga ttttgtacgt tttgttggca acaatattaa atgttgccaa gccattgctg
    19021 tccgtttttt gtacaacttt tacacctttg tcgtctgtca aagtgaattc tgcgtttgct
    19081 aatgctttcc cttgatcatc cactttatga actttgatca cacctttttt caaagtgttt
    19141 ttagcagtat attcaaagac ttgtccatca tctttgacgg taattccttc ttgtttgaat
    19201 gcgcctgtgt aacctgttgg tacttttgtt tcttctaagt tataagtttt gttcgcaata
    19261 atgttgaacg tagccagacc agttgcatcc gtctttttaa cttcttttac acctttttca
    19321 tccgttaagg tgaattctgc atctgctaaa ggttttccat cttgatcgac tttatgaact
    19381 tttactacac cttttttcaa attatttgta gctgtatatt caaaagtttg cccatcgtct
    19441 ttaacggtga taccctcttg cttaaatgca cctgtgtaac ctactggtac tccggtttct
    19501 tctaggctat acgtcttgtt tgctacaata ttgaagcttg caagtccatt ttcatctgtt
    19561 ttttctactt gtgttgcacc tttttcatcg gttaaagtga actctgcatt tgccaaaggt
    19621 tttccatctt gatcgacttt atgaactttt actaaacctt ttttcaaagt gtttgtagct
    19681 gtatattcaa aagtttgtcc atcatcttta acggtaattc cttcttgctt gaatacgcct
    19741 gtataaccag ttggtactcc ggtctcttca aggctgtagg tcttgtttgc tacaatatcg
    19801 aagcttgcaa gtccgttttc atctgttttt tctgcttgtg tttgggatga atcatccgtc
    19861 aaggtaaatt cagcattagc taatggtttt ccatcttggt caaccttttt aacttttact
    19921 ttaccttttt taattgtgtt cgtagcagtg taactaaatt cttgtccatc ggtattaaca
    19981 gtaataccat cctttttaaa tgaaccttga taaccttcag gaacttttgt ttcttctaag
    20041 ctgtatgtta agccttgttg caaatcaaac gtagctaacc catcatctcc ggttgtctta
    20101 acagatttag cattgttatt gtcagttaga gtaaactccg ctccttttaa agcatttcca
    20161 tcttgatcaa ctttcttaac tgttacctga ccaacagctc ttgtccattt agcactcaaa
    20221 gatttagagt cctttgtttc tgaagtagtg atcataacta cggcatcttg atacacacct
    20281 cgtccatcaa aagtaaaagg attggttgtg ctaacattag cagtaccttt aaatgacaaa
    20341 gtaccacttg gtgtagtcac aggtacttta atagtaaaac ttgaatttaa tttcacagtg
    20401 ttgttagtaa tttctttacc atctgcatca taaatcttca taccagctgg ttttgctgat
    20461 gtaaaagtga gcttcgcatt accagtctta tttgatgctg ttttatatgt gaaagtgtga
    20521 tattttcctt cgtcagctgt cttagaagct gtgatagaaa actcagtgtt gggcgtatct
    20581 tttccattct tagcatagtc ataaattttt tcaaatgctt tttttacacg agctgtttct
    20641 gcggcggtat ggtttggatg tgaccataca atctgtgaaa ctttaaagtt tccggctaaa
    20701 acccatgaaa ctaattgtgt tgcataccat gcttctgtat catttcctgc acccagttcc
    20761 tttgctgttt ttgatggaaa accaccttta aacatacgat agaactcatc actgccagct
    20821 cccttgtact tcaagcttcc ggctccactt cctgttgggg atggcaattc tgaatccata
    20881 caatatccaa tatttccatg agaatcttta aattgacttg aaatcatcca ttcatttgga
    20941 tgattcggca tattaaccct aatataaccg tctgttttta cgccagtttc tataaatttt
    21001 gcacttgtcg gttttgtgac atctgcttca gctcttattg ctgaaaaagg cattataagc
    21061 gtagataaca tcattactaa agtcgcaaaa caataaatta tttttgattt taaaattatc
    21121 gttttttcat gcttcatctc tcgttactcc ttctttatac cagttaggcg aagaaaatga
    21181 gccacgatat gtcggtgtgg taatgacctt cgcgcccatt cctaagttaa aatcaccaca
    21241 cctattaatc atttttttcg ttgaaagcaa gctgtttcaa cgctctagta ttcatagcat
    21301 cacccctttt agttctaccc accatgagca acaagttttc taagtagtta agcaaggaca
    21361 aataaagact atataactac ttagtatata aacgatatct tttattaacg taccgaaacc
    21421 gctaattgag atacgttata tgctgtttca aatgatcctg cattaatcca acttgcccca
    21481 aacacaggat cgacaacatg gtaaaaaccg tctttaaatc catcaacggc tactacatgt
    21541 cgattccaaa tagcatcgcc ccaaaacatt ggtttatgtt gagcaggttc aaaggcataa
    21601 gttacccacg ctactacagg atttccattt acaatctcat cttttaatcc ttgtaccgat
    21661 gaaccagaaa tatctacaac atttccgcca ttagcaacac cccatgaaac tacaggagcc
    21721 ggattcatac cttgataagt tccatccaca ttgtgtctcc attcaccaga aaaaccatta
    21781 tttggattat tgtcaggaga tagtggcatt gatttaacaa aagtcattaa atcttgattt
    21841 gttgcaacac ctttataatg taacgcttct agcaaagatg ttccttcgca aagcattgtg
    21901 ttaccttgac taataaatgg aacgcctagc acaacattag ttggttgttc gggtttactt
    21961 ggtgaagtag aactgtcagt ttcatcactc gaacctgttt gtgattgatc actagaagat
    22021 gtactggttg attcatttgc atcatttgat gttgattgtg agttatttgt tgatgttcca
    22081 gtatcagctt ccgtagtgct actttcactt gttccctgtg atgcttcttt ctcacttgta
    22141 gaattatttg tattagatga acttgctcca tcaacacttg aagcacttgt ttctgtctta
    22201 tcagaagtgt ttgctgttgg ttccgattct tttgtagtct cggatttttt gttctcttgc
    22261 tccgtatttt caataggatc aggtttatct ttttgaacag tttgactaac tacttcgcca
    22321 tctgcttcct taactgttaa tgtatcttta ctgatgttga aagttcgtcc aatcgggata
    22381 gtccgttgtt ctccttttgc caaatcaaca ttgttcaatt cggcaaactc agcaacagta
    22441 gtcttgtaac ctaagctttt aagcttctct acagttgacc aaatagtatc tccttttttg
    22501 aacgcataac ttgattgtcc tgttgtaatt tggatttgat ccggtgtgtt tgcagtccat
    22561 tcatcagcat gtgaggttac tggtactgat aaccccgcaa gtacagctga tgccaataaa
    22621 accataccaa gcgaatttct cttcatattc attccttctt tttgttttta ttttctgttt
    22681 gtgatcgcaa tgcttctatt ccttcttccg ttataatata ggttttgtca cccactttct
    22741 tgaacgaacc tttaggcaaa cgatttggat aatagttgat tatgtttgta acataatttc
    22801 tacctttacc caaaatcaaa cttgcttccc ttgcggtata caagttgtat ttgtctaggt
    22861 tcaatttctt tttgtttgcg atttagacct acccctttcg caaaatttat tgtataataa
    22921 tagtaccatt gcactgtcat aattgcaata ttttttgggg tttatcttca tttaatagcc
    22981 taaaaaggct tgttagataa ggaatatcta actaatcaaa acaaaaaagg atgttaagaa
    23041 aaaatgaaca aaaaaaggaa attatcagta ttaattacac ttgtttctat attgactatt
    23101 cttagcgtta tcttgtcgct gaaaatattc aatcaaataa acacaagctc aaatgccaaa
    23161 gatggaatag tcgtagaaaa tacctctaat tctaaagaaa ctggtactac tacaaaaaaa
    23221 tacaaatctg ctgagatcac tacgaaaaat tatttaataa agactgttta ttttgatgaa
    23281 cgaaaaacag caattctcaa agatgaggaa ggatcagaat taaagctacc tataacggta
    23341 tccgataagt tcaatggaac gttctcctta caaaccgctg aaggatcacc tgacaatctc
    23401 aaagttgggc aaacaatcat tcttggtaaa aacgacgaac tgtattcatt tgtaaaataa
    23461 aaaaatggag caaaggctag tcaagccctt gctccatttt ccattcttca agaggtgtag
    23521 ctatttcaac accaaagttt aaatactgtg gatgctgtag atacatgata tccattctat
    23581 tttcgataaa ctcttttaac gttgagtaga tgtatttttc taatgctata tctgcccaac
    23641 taccataatc aagtttattc gtgatcttga cgtttatttc aatttgagaa acatcaatat
    23701 tttgaacatc aggaaactta ttcagtctta gctcttctct aaccgcttct actagcggtt
    23761 catattgtcc gcctagttcg ctaattgttg aaactatttc atctgaattc caacgatctt
    23821 ccaatacctt tgctacattt tctttaggcg gttgtctttc tataaattct tcaggtgttt
    23881 catcaaactc tactctaaag ttcaatttat tttgattgtt agcatacata taatcatctc
    23941 tgtttgattc aaaaaagtta cgaattcctg tgtctgattt atcacttaac tccttatttg
    24001 ctaaagtagc ccaaaaatcc caagttcctt tattttcagt atacaaagta aaattaatgt
    24061 ttatatagtt tgcagtttca ggataccctt gctctctaag agcatcttta acaatcactg
    24121 ctaagtcagt ttcaaaattc tccataaaaa attcactaaa ttcttttgga ttccacgttt
    24181 cttctaataa cttttctaat ttatgatctg ccattattat tctcccccac tttactttca
    24241 atcttatatc taaaactgct taaaattaag agctatacct tatcataaaa ggtattttat
    24301 gctttattta agtctacaat tctatattac aattatcatc tagaccattt tcttatgtca
    24361 aataaaccat atatattgtc tttaatagtt ttatttgact ttttaagaaa gtatttactt
    24421 ttgacttttt ttacgtttta caacatatac gtattgtaat gttcagctca atttattata
    24481 atctaaatga attgattaat aaaaattctg aggttcgggt tttttatcaa ttcttttctg
    24541 tttgtgtttg tgatgaaaca atcctttttc ggtaggtttt gttatcatcg tcagcacaaa
    24601 gagctaacgt tggtaccgtt agctcttttt ttattttact ctgctcttac atagctatct
    24661 aaatcctcca aataaaacaa ttgattttct cctatgcttg ttactccacg ccaaattgtt
    24721 gttgttgctg ttgttggagt atttcttgtc tgtgtaatca attcaaccat tttacctttt
    24781 tgatttgttg tatagtaatc agcagtatag ctagatattg gactattacc agtgcctacc
    24841 atcgtgccag aaatatcaac aggactagac tttactatct gaggaaattc tttttgtatt
    24901 tccttcatat tcttcgtata tttttcccat gaattccagt tgtaaaataa attaaagaat
    24961 ttcctacttt gttccttaat ttcagcattt cccaattctt tgtattgagc ttcctgcata
    25021 gctttcactt tctggttatt cttgactact tcgatttgtt tacttttaag gtcttccaat
    25081 tctttgacat tattatcata agcatttttc ttttgaaata gtaccacaca acaaaggatc
    25141 acaattatgg aaagtattcc gatcgccttt ttagcagtag tgatttcatg attttgcata
    25201 aaaagcaccc ccagtcactt tcttatcatt atttagtgtg atggttaatg tggtatcata
    25261 cacttttttt ccattgtatt gaacttcaaa atcggtcaat gcttcataca acccactact
    25321 agatgtctta attccaaagt ttgttagctt aaaagtaaaa ggcttatagt actcgatttt
    25381 ttctttctca ctactcttat cagtaattgg tggtttacct gttacaataa atagttttct
    25441 caaaaattct tcacctgatt gatctgcaaa cttattttga actgatccat tcaacatttc
    25501 ttgaatatca ttttctaaat ctttccgtaa tccatccacc tcatttggtt taacctcttt
    25561 aaagaatgtc agcttatctt caatatgcac actgtgtttt tcagcttcta tgtcctgatt
    25621 ctgcttattg aagaatagga ttccaacaag cagtgctaat aacataccag aaagcaaaga
    25681 aatgataatt gtatctttca ctttactatt catctatatc gtccttcatt ccaatttatt
    25741 tttcgatttc aaatttagct tttcctacac cgctaaagaa atgcacatta tctgatccgt
    25801 ccactccatc aatcaacgtt accgaaactc cccttgttgg agcttgggtt ggtggaacat
    25861 taaagttagc acatagaaat gatccatctt caaacaccgc tataactaca ccagtatgcc
    25921 ctgcatctcc aacagcccat aaccatccat ccgaagcact aaacccataa cctacgattg
    25981 gtttgttcgt tatttttgct cctgccgatt tgtagctttc ccatacataa ccaccatttc
    26041 cagtagtcgg ttgattccca tcgtataatt ggctcatata agcccacgtc aactctgtac
    26101 actgaccttg atatattccg ttaaaaggtg aagttgacca atctggttct ttgaattttg
    26161 gtagcgtgat cgctttccca tattttttca aagcttcttc ataagtcata ttcgctatta
    26221 cttgaccttt tttaccgtta atttttcctt ctttcccaat tgatccagaa ccagaattag
    26281 cgtcatccgt agttgcacag gcatcttcta ctgttcctga tgctgaactt gatgaattat
    26341 tcgatccatc tccggtactt gaacctccgg atattttatt gatcttactt tcatcttttg
    26401 caccgtccat acccttgctt ttcatgtact tccagatttc ttttgcattt tcaccacgta
    26461 cagctattac agctgattcg gaatcagctg aaatttccca gtttttatga aaagttactg
    26521 cattttctac gacatcatca gaagcattta aagcataatc ttttaatatt cctacgaatc
    26581 ccccatcacc tttgaacata aagtccaatt gggcttctgt agtccaccaa gaaccgccat
    26641 tatttttctt agaccaatca actaatgctg tatgtcgccc agctgtccat tgaccatagc
    26701 caatacccat cagaccatca gtaaattgtt tgatgaagtc aacttctctc tgtccatctc
    26761 cgtaataccc ttctactgct cgaggatcaa tacctgattc ttgtttccag ttacctaaaa
    26821 tacctgcaat actagcacct gataagccat attctccatt catgtatttg taaatatctt
    26881 caatattttt ggctacatca cctgttacag cgccacttcc agtatcgttt ccatcgctat
    26941 ctgttcctga cccactacct tcagtagtac agttaaattg tgcatcataa gtagctatcc
    27001 catcagcatg tatcacttgc ggatttattg tgattacact cattgcaatc cctgtaaatt
    27061 gcaccattaa cataagcaat gtggcaatct ttctcttaga ttttttcaat aactacgccc
    27121 ccaaaaagtg tactttttta tgttattatg ataatataat aacataaatt aatagaaagg
    27181 aaaagctaat aaatgactga taaaacaagt gatttaccta aactagctcc aaaaacatct
    27241 cgagtaaaga gcaaaaaaga tggaaaaaca aaaaatgctg taaagaaaaa gtcagtaaaa
    27301 gaaacatcct cgttactact tgatattcca atgagttctt ttgatgacaa tacggacgaa
    27361 acttctgtta aaattgcaac tcacgttgga atgaacaatc aaaatgattc tttttacaac
    27421 ttgttaaaca gcctaagaga gttggaagat aaaaaaaatc aggtaatcgc atcaaaagct
    27481 cctgatctca caaccgattt ggatgctgaa attatccaaa tcaaacaaga tgagcgatca
    27541 gttaaatcgc tactgaaaac ctactttgat ggttttgata aagaactagc tccttatagc
    27601 caagcaatcc taactaaacc aatacaagct ccacatattt caatttccct acaaaaaaag
    27661 agtgatgatt aaacaatcat tactcttttt tagatttctg gtaagcgctg atcgcaaaaa
    27721 ccatcaacaa tattgattca aaaaatatgt tcattcgtgt ctcttttttc cattatagat
    27781 ttcccactat tccattaaat cgaaagtcac accgattact acaaagatta acgtgaccac
    27841 attcaaaaac attaaaagat atgctgataa tctcatcatt tttttagttc acttactggt
    27901 aatctaaaca tttgaacgat tgcactggaa ataccataat tagcaatgac aaacaaaaaa
    27961 tacgcaataa tttcacttac acctagatag atcagtaaaa taaataagtc acttaacatt
    28021 gttagtttcc tcccagtata tgattttcat ttttgggaag tcttttataa tccaattcct
    28081 caaaaaaagg aaccatagtg tcactaatat aggttttagt ttcaggtacc gccaaaattc
    28141 cccaatcaag aatttcttcc catgatacat catcaaattc taaaatatta tgatcaactg
    28201 acctattacc aagttcatat ccaccaaaga tcaccatatt atcaatcatg tatagaattt
    28261 catcaggcga accatgagtt aatgtttcgt cctcttcaag cagttcgttt aatctttgtt
    28321 tgtattcctc tcttttcata cttttatccc ttctgactca tttttttgaa ttttaacatt
    28381 tttaacccaa gcatgactat ctttacatgc tattaatttt tatctccatt ttgatatttc
    28441 tgtttttttc tactcaccaa tcaaccgtaa atcctcaata ttctcacctt caagcgaata
    28501 tgaatatact tcgccgtaaa tattttctat aactacgact tcttcttcaa agtctatgga
    28561 ctgcacatca aaccattctc ctccgccaaa ttcgtctatg attagtacct gcaatatttt
    28621 atttactgcc atttcaattt ccccttatac cattctatgt cttccaaact atttttcttc
    28681 tttccatacc aaggtttcac gtatctcaaa attttcttta aataagtgtt ccggtacttt
    28741 tataattaac tcttttccta cttttatcag atagtctttt tcacctaatg agatttgacc
    28801 agtcggtaac tgtatagaaa ctcttttagt tttattaaat tttttagttt tcaatacagt
    28861 atacggtcta cagaatttct ctaagcgttc aaatctttta ctatcgtatt ggaaataagc
    28921 tttaggaaca gataaagtac aaaaactatc ggtaacagca cttatgtctt ttttagaaat
    28981 tatcccaact ttagaacttc taaggaaagt tgctaatttc ttatatccac catgacttgt
    29041 ttcaatatag atgctattgt attggctgtc gtctatctca tttattgttt caaccttttt
    29101 cctgacaggt gtttcaacga gttctcgaac ctcttcttca ctgtacgcta atacaattaa
    29161 ctcttcccca tttaccatct ttagaacata cgcttgcttc ttttttcttg gcttattttg
    29221 agattcaaca taaccagaca tggaatcttt atacttccaa gcatgtactt ttttctctaa
    29281 tttctgtatc tcttttttca gctgtagata ttcagacatt ttttcattgt aacgttcgca
    29341 atataaacaa tattcatctt ttccaaaatc atgatcaagt ccttcttcaa tatgtttatt
    29401 caacaaaccg gaaaaatact gatcatactg cttctttcgt aacttcagct ctcgtaaatc
    29461 ttcagacatt tcatttttcc tttccaatta ttggtgccat ttcacaattt gggattgact
    29521 aaacaccata attaatttgt aagctaacac aattacaact gttggtaatt gtgttagtct
    29581 ccatcaactt gtctggtggc tttttttatt gcacaaattt catagctctc aaaaagcgct
    29641 tggcttgaaa aggtgttatt ctacgaacct tattcgccaa tttccaatct gataaagcaa
    29701 tttgccattc gtgcatttca aaatccggca tgatttcttt gatatactgt agctctttct
    29761 tttctctttc ttttaagaaa cggctcatcg agtttaacat tggaactaag gatgcgtagg
    29821 tacttgaata acttccaatg cgtttcgtag taattacata atcaataaaa acactattaa
    29881 gaaaggtcaa gtttataaac tctgattcat acactctaaa atttgcagtt aatcgcttcg
    29941 ccttgtcacc ccaatagtca cttttgttgt aggctttttc ccctcgaata tagcactctg
    30001 tttctctttc gtaataatag tcatactttt ttcatcatca aatctgagag tttttgatta
    30061 tctttttcac tgtaaaataa tctactcgtt aaacttttag gataatcttt gatagaatat
    30121 gcatcccaaa caattctttg aacatattca cgatctatat tttctctgac ttccccagtc
    30181 caaaaatgtt gaccataatg gcatctttct attacattgt tagcagaaac ttcaaaatag
    30241 gattgttccc tttgtatcct cataccaatc atcatagcgc aggaaataac tattaataac
    30301 aatcctgttt ttaatctatt attccacatg cagtttccac atccttatct tgataaaaat
    30361 cctcttgacc ctctccaatt actgttttaa atataaccac caaaacaaga tcatgctaag
    30421 aaatccaaga gtatataaga tgatacattc tttgttaatt atccaaaaaa ctctgatttc
    30481 tttgagcttt tcacgcatta ggaaacacct tgttatatct tttttgtatt tctttcaatt
    30541 gttttttaaa actcataaac aactgttcat gatctggtct aagcgttaga atatctaaat
    30601 tatttatctt ttttaattgt gtaataaaat ctattttgga ttttctttca atatcaaaac
    30661 cgacaataat ctttcctgtt ggatcaacgc atttcacttg aataactttg tcattttgcg
    30721 atagctctaa tattgatgat ttttcttttg cctcttccaa aagatgttct aacacttcgt
    30781 taggtgttcc atgatagtaa taaatcatgg tcttctcgaa aatttttatc ggatcaacac
    30841 catattcttt tccgattcga taaaaaaatt tcattggaat ttcttccgga tttctgttcc
    30901 tgcttgacca gttagagatg gtttgataat ttgcaaaatg gattcgttcc gctaatctct
    30961 gaactgtggt ctctaaatct tcttctatta attgttttac agcactaatg ttttgtgaca
    31021 tttgctttct atcccttcaa attcgttgca ttatcaattc taagatttca tgttttagtg
    31081 catttctatc tacattttgc ttttttaact ctgcaccata atatcttcgc aatcccttat
    31141 ccaagcatac aattccttga tctaatagat catattccgc tttttcaaac cttcgctttg
    31201 cctgctcaac aagcaaaatg atattgtgat gtatctgatt aaactgccga tcatattcct
    31261 ctaaagtttt agcatctaag ttagcaatat gatcctctgc taatttttgt gttaaaaata
    31321 attcgacttt ctcagatttc aacatctgta ttttttcata ctcttgtttt gcttggatat
    31381 agtcatcttt caagctagac atctaactca tctccttgcc tgtataaact gatacggact
    31441 gtccaaaatt gaataggttg gtgatgactt caagaagact tcaccttttt cacatgaaaa
    31501 taaaccaatt aattcttcaa tttcatttac gatcagatat ttattagtta aaattttccc
    31561 attttctttt tctagctcga caatcgcttg attccatgta ttttgcacta aatcttgaat
    31621 caatttcaat ctatccactt ttttcatctc atacagggca atttcagctt gtttaatgcc
    31681 ttttaatttg cgaaaggcat atttgtattg tgtgtaggtt aaaaagcctg tgtagttata
    31741 catgggcttt cctgatgtct tgtagtcatt taagcttgga tatatttcag ttttccattt
    31801 ttcttctgaa cgttgatcaa taattcttaa tttttcaggt aaatcaattt catagtattc
    31861 ttcaattttc agctcacgat aaagtagatg tttttccttt tctttttcaa ttacaaaaga
    31921 attatttccc ttcagtttag ctatttcttc acttgtgaga ccaagcctaa ttttttctac
    31981 ttcacttcta agttcacgaa tacttgttgc ctttaaccac ttcccatttg caccattatt
    32041 gtaaccggta agtaactgta ttttataaat tccttcacca tgaagcacct tatatatacc
    32101 aacctgattc ttttgcggaa tatgagcttc atttgcactg atttctttca ttacgcattc
    32161 tcctttataa tctagttaat tagcttgtgt gtttcgagta gttgagtaag aacgtttgtt
    32221 tcagaaatca tttatttgtt atcacttaat
//
LOCUS       DACCWV010000015        32111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCWV010000015 DACCWV010000000
VERSION     DACCWV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.015"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..247)
                     /locus_tag="IVW84_000648"
     CDS             complement(<1..247)
                     /locus_tag="IVW84_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7916868.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSE"
     gene            complement(250..1146)
                     /locus_tag="IVW84_000649"
     CDS             complement(250..1146)
                     /locus_tag="IVW84_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7916869.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(1304..2146)
                     /locus_tag="IVW84_000650"
     CDS             complement(1304..2146)
                     /locus_tag="IVW84_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7916870.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            2278..2931
                     /locus_tag="IVW84_000651"
     CDS             2278..2931
                     /locus_tag="IVW84_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7916871.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(2994..3521)
                     /locus_tag="IVW84_000652"
     CDS             complement(2994..3521)
                     /locus_tag="IVW84_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7916872.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3541..4488)
                     /locus_tag="IVW84_000653"
     CDS             complement(3541..4488)
                     /locus_tag="IVW84_000653"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7916873.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(4500..6386)
                     /locus_tag="IVW84_000654"
     CDS             complement(4500..6386)
                     /locus_tag="IVW84_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7916874.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(6546..8624)
                     /locus_tag="IVW84_000655"
     CDS             complement(6546..8624)
                     /locus_tag="IVW84_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7916875.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(8637..8912)
                     /locus_tag="IVW84_000656"
     CDS             complement(8637..8912)
                     /locus_tag="IVW84_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916876.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(9274..9729)
                     /locus_tag="IVW84_000657"
     CDS             complement(9274..9729)
                     /locus_tag="IVW84_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916877.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(9883..11091)
                     /locus_tag="IVW84_000658"
     CDS             complement(9883..11091)
                     /locus_tag="IVW84_000658"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7916878.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(11203..11535)
                     /locus_tag="IVW84_000659"
     CDS             complement(11203..11535)
                     /locus_tag="IVW84_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7916879.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            11683..12558
                     /locus_tag="IVW84_000660"
     CDS             11683..12558
                     /locus_tag="IVW84_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7916880.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(12640..13221)
                     /locus_tag="IVW84_000661"
     CDS             complement(12640..13221)
                     /locus_tag="IVW84_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7916881.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(13227..14486)
                     /locus_tag="IVW84_000662"
     CDS             complement(13227..14486)
                     /locus_tag="IVW84_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7916882.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(14488..15534)
                     /locus_tag="IVW84_000663"
     CDS             complement(14488..15534)
                     /locus_tag="IVW84_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916883.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(15769..16044)
                     /locus_tag="IVW84_000664"
     CDS             complement(15769..16044)
                     /locus_tag="IVW84_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7916884.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(16287..17597)
                     /locus_tag="IVW84_000665"
     CDS             complement(16287..17597)
                     /locus_tag="IVW84_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7916885.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(17779..19008)
                     /gene="rpsA"
                     /locus_tag="IVW84_000666"
     CDS             complement(17779..19008)
                     /gene="rpsA"
                     /locus_tag="IVW84_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7916886.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(19143..19823)
                     /locus_tag="IVW84_000667"
     CDS             complement(19143..19823)
                     /locus_tag="IVW84_000667"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7916887.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(19872..20507)
                     /locus_tag="IVW84_000668"
     CDS             complement(19872..20507)
                     /locus_tag="IVW84_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7916888.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(20573..21913)
                     /locus_tag="IVW84_000669"
     CDS             complement(20573..21913)
                     /locus_tag="IVW84_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7916889.1"
                     /translation="MIQALLDGEDTLAILPTGTGKSLCYQLTGYLMEGLVIIVSPLLS
                     LMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLSQYKFLFLSPEMLTNPSLLEHL
                     KKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLLGNPVTLALTATATDLVARDIR
                     HVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHFMERAQGAAIIYCATKKEVERL
                     YHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFLIATNAFGMGIDKSDIRYVVHY
                     DLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIHYFFNQLSREQRQSFELYLEYA
                     TEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEKEFRLQQMLRYINEENCRRKFI
                     LAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQLHWETILLNLFKKNMEH"
     gene            complement(21964..22995)
                     /locus_tag="IVW84_000670"
     CDS             complement(21964..22995)
                     /locus_tag="IVW84_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916890.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            23026..23247
                     /locus_tag="IVW84_000671"
     CDS             23026..23247
                     /locus_tag="IVW84_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7916891.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(23412..24149)
                     /locus_tag="IVW84_000672"
     CDS             complement(23412..24149)
                     /locus_tag="IVW84_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7916892.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(24302..25642)
                     /locus_tag="IVW84_000673"
     CDS             complement(24302..25642)
                     /locus_tag="IVW84_000673"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7916893.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(25879..27402)
                     /locus_tag="IVW84_000674"
     CDS             complement(25879..27402)
                     /locus_tag="IVW84_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7916894.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(27780..28391)
                     /locus_tag="IVW84_000675"
     CDS             complement(27780..28391)
                     /locus_tag="IVW84_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7916895.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLMLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(28514..28639)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(28721..29437)
                     /locus_tag="IVW84_000676"
     CDS             complement(28721..29437)
                     /locus_tag="IVW84_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7916896.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(29443..30012)
                     /gene="scpB"
                     /locus_tag="IVW84_000677"
     CDS             complement(29443..30012)
                     /gene="scpB"
                     /locus_tag="IVW84_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7916897.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(29996..30793)
                     /locus_tag="IVW84_000678"
     CDS             complement(29996..30793)
                     /locus_tag="IVW84_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7916898.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(30763..31134)
                     /locus_tag="IVW84_000679"
     CDS             complement(30763..31134)
                     /locus_tag="IVW84_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7916899.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(31153..32040)
                     /gene="xerD"
                     /locus_tag="IVW84_000680"
     CDS             complement(31153..32040)
                     /gene="xerD"
                     /locus_tag="IVW84_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7916900.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         8967 a         6807 c         5444 g        10893 t
ORIGIN      
        1 actcactaag gaaaaagctg atgatttgat tgactttttc tttatttgaa ttgatcgtta
       61 agacaggatc tccttgtctt tccgtaagag ctggtatttc tgggagttct ggttcccgat
      121 tagcaaaaat acggctgcct aaaaatacgg tcgaaccaag tactacccca agaaggataa
      181 ggaaggcgaa tttccagtaa tttgttttct ttgttttgtt tgaagatacg tcagatcttg
      241 ttttcatgtt tattgatcat cctccttctc taaccattgg ttttgtgtct cgatcagctg
      301 atctctcact gcatttgcca tcaattgata acctaaatta tttggatgga aatgatcttc
      361 ttcatagagc acattatttt tgatgccatt tagattttct gtcgtgctag ttgtttgatc
      421 tgttccttct tctgtgacac ctacttcctg attcaatcct tgataaagca aatcattgat
      481 tgggataaag tagcagttcg gcattttttt tgtcaccgtt tctgttcctt tgttccaatt
      541 gttaacaatt gtctgcatgt cagtgatctc aggaaagttc aaataaaatg gattgtagat
      601 gcctaaaatg taaataggtg cggttttatt gagggatcga aattcttcca atagttctgt
      661 gacattttcc tgatagtttt tcagtggctt tttaaaggaa ttgaccgtta acccgaataa
      721 attattttgg ataactttca tcaaatcatt accgccaaca gtcagtgtga taaaatcagc
      781 tgttttgata ttgtttcgaa tttcttcttt tgtttttact cgctttaaga tctggtcact
      841 tcgttcacca tttacaccat agttttctgt ttcaaccgcg gtcaaattat aacggtcttt
      901 tagatcattt gcgacaaggg gaacgaatcc tccttgcttg gtttgatctc ctactccttc
      961 agtcagcgaa tctccgatag ctatgtagtg aagtttttct tttttatttg gatcttctct
     1021 cacagtagtt actggtttca ttagtgggcc agctttcggt atagaagcag ctaaacctgc
     1081 atagatgatg atcgcaatta ataatgggaa caaaacgagg atcaaatgtc gtttcgttaa
     1141 tttcattttt tcacctcttg tattcgattg gttctaatgt tatcaaaaat tttatcaaaa
     1201 aggtagtctt tatgaaaaaa gaaaacaatt attttcctaa ttttataaag aaaaagagaa
     1261 taagctgcaa gcctttcagg ctgagggctt atcctctttt cgactaatct acataataca
     1321 tgattgcaaa cgcgtcttta ccagtatgcg tagcgataac cggattcgta tgcagtacag
     1381 ggatatgcat gtccgggaat aattcttgca aaccctcttt gaattcttgc gccaattctt
     1441 ttgcatccgc atgtgaaata ccgatttgtc gcacatttgg caattctttc agttcatttt
     1501 tcaaattatt aaaccattta tcgaacgttt tctttcctcg ccccttagtt acagggatta
     1561 attcagaatg ggcgaaattc atcaccacgc gtatgttcaa aacactagac agcaaaccag
     1621 ttgttcggct gattcttcca ccttttacca agttatctag tgtggatatt ccgatgtata
     1681 attttgtatt gtcttgtact tttttgactt cttcaaggat tttctctact gttttacctt
     1741 cttttgctaa ttcagctgct cgaattacct gaaaagataa tccttggtca gtggtatcac
     1801 tgtcgataac cgttactttc gtttttgtta gattactggc ttgtcttgct gcttctactg
     1861 ttccactcaa gccttttgtc atatggatcg aaatcacttc actgccgtct tctcctagct
     1921 catcatacat cgagatgaat tctccaattg gtggttggct tgttttgggc agattttttg
     1981 ctttcgccat catttccata aatctttcgc cgtctagttg gtcatcatcg ggatatactg
     2041 ttccgtcgat catgatggat agcggcatga cacgaatgcc taaagcatcg cgtacatttt
     2101 tttccatcgt acatgaagaa tctgttacga tttttacatt tgtcattatt ttctctctct
     2161 ctttctttaa caagatgcct tattcatgtt aaactacatg taagacaatt tctattttta
     2221 gtataacaga caaataatga tttttcatta attacaccta gtaaaggagc cgttttcatg
     2281 gaaaaaacga aatttaatcg aacgtatgaa atattaaatg aagtgtttaa tgcagtcacc
     2341 catggaattg ggactggatt gagtatcgcg ggactagtgg ttcttttagt aaaaggtgcc
     2401 cacttgcatt cctccctcca tattgtctct tatgctatct atggatcgat gatgattctt
     2461 ttatttttaa cgtctacttt gtttcattca ttgattttta caaaagcaaa aaaggtgttc
     2521 caagtattcg atcatagttc gatttttctt ttgattgcag gaagttatac tcctttttgt
     2581 ttgctgagta tccgaggatg gctaggttgg actttatttg tattaatctg gttgtttgcg
     2641 attcttggca ttatatataa agcgttgacg ttgcataaac gggagagagt atcaaaagta
     2701 tctacgatca tctatatttt tatggggtgg ctctgtgtcg tggctgctgt tcctctttat
     2761 cactctcttg gaatgactgg tgttattttg atggtagcag gcgggctttc ttacacgata
     2821 ggcgcattct tttatagctt gaattctatc cgctatatgc atgttgtttg gcatttgttt
     2881 gttatgttag gtgcaggttt tatgtacttc tctgttttat tctttactta ataaaaaaaa
     2941 taaagctatg gatgggacag ctctaaataa cagctagtct atccatagct tttttatatc
     3001 gtcaattcgc ctgtcccgct ttttacagga ttctcattac gctgatacgt ttcaaattga
     3061 tgcggataac gatttttttc atccatttct ccttgactag tattgatcag tgaccaatca
     3121 gaccagtcaa caggtggaaa ataagcatct ccatcgaata catgatggat gactgtacgg
     3181 tataatatcg tacaaaatgg cagaaattct tgataaacag ctgaccctcc agcgatgaaa
     3241 aagataccgt ctgtttcttt ttccagagca agcacgtctt ccacactatg catgacatgc
     3301 acgccttctg ctttatagga agaatctctc gtcaaaacaa tcgtctgacg attcggcaac
     3361 ggacggctac ccatcccttc gaatgttttt cgtcccatca ctagtgtatt tgcttctgtc
     3421 atttgtttga agaatttcag atcatttggc aaacgccaag gaagacggtc ttcttttcca
     3481 atcaatccgt tttcatcctg tgcccaaatt gcagctaaca ttttctcatc ctttctgatt
     3541 ctaaacagcg ataggagctt tgatcgccgg atgtgggtca tatccttcta attggatatc
     3601 ttccatctca aaatcaaata ctgactgctt ttcctgattc aaaactaaag tcggaaatgc
     3661 gcgtatttct cggctcaatt gttctttcat ttgttcgata tgattgatat aaagatgagc
     3721 atctcctaat gtgtgaacaa attcgccaac cgctaaacct gtttcatgtg caattaggtg
     3781 agtcagcaac gcataacttg caatgttgaa cggaacgcct agaaacacat ccccactgcg
     3841 ttgatacaat tggcagctta gccgtccctc ttgaacataa aattgaaaca tcgtatgaca
     3901 aggaggcaaa gccatgctag gaacatcttc cggattccat gcagaaacga tcaatcttct
     3961 agaatcagga gtctttttga ttgcttcgat cacttgtttg atttgatcga taaagccacc
     4021 gtcttttgtt tcccagtgac gccattgata gccgtagatg tgtccaagat cgccgtatgt
     4081 ttcagcaaag gaatcatcgg ttaggatctt ctcgcaaaaa agcttgctct gctttttata
     4141 ttcttcattg aattcaggat ctgtcaaaca tctatggcca aaatcagtca tatctggtcc
     4201 attatagtct ggacttttga tgtagcgttc aaatgcccat tcatcccaaa tatggttttt
     4261 attttgtaat agatagcgga tattcgtatc tccttttaaa aaccaaagca attcgctttt
     4321 gatcaatcca aaaggtactc gtttcgttgt taacaaagga aacccttttt gcagatcaaa
     4381 ccgcatttgg tatccgaata agcttaatgt gcctgtgcct gttctgtctt ctttacgatg
     4441 cccttcttcc aacaattttc ttcctaagtc aagatacgct tgttccatcc tttttcctcc
     4501 taatcttcta cccattcact caagtattcc caccgtgcca ttttttcttc aagttgtgct
     4561 tctgtttcag atacgtcgtt ttgcaattct tgtaatcgag tgaaattatc tccttgatga
     4621 ttcatctctt cttggagcaa tgagattttt tcttcaagtt ctgcaatctc atcttcaatc
     4681 gtttcccatt ctttttgttc catgtaagat aatttcttct tttcagcagg ttccttgctc
     4741 tgtatttttg gtttcgcttt ttcagcaggc tgctgggtct ctttttgctt cagtaaataa
     4801 ctggacatgt ttccgtaata aagttcttgt tttccgtttc cttggaatac taataactta
     4861 tctgctacct tgtccaagaa ataacggtca tgggaaacag tgatcacagc acctggaaaa
     4921 cttctaaaat aatcttccaa aatcgtcaat gttgctatgt ccaaatcatt ggtcggttca
     4981 tccaataata aaacattagg ttgctgaatc agcagtttca acaaaaataa ccggcgtttt
     5041 tctccgcctg aaagtttgcc tatcagtgtc ccatgcataa atcttggaaa taaaaatcgt
     5101 tcaagtaatt ctgctactcc gatttgactg ccatctgctg tttttgcttc ttcagcagct
     5161 tcttgtaagt aagcaatcat ccgtttatct ggtgccattt cttcattttc ttgtgtataa
     5221 tacgcaagat gaaccgtttc accgatagac attgtaccgc tatccagggg aattctgcca
     5281 gctagaatat taagcaatgt cgattttccc gcaccatttt taccagtaat acctagtctt
     5341 tcccgagatt gaatcaataa atctaaatgt tctagcagtg tttggtcatt tatacggtaa
     5401 ctgccatctt tgatttccag tacttttttc cctaaacgtt gtgtcgctag gttcagctct
     5461 aaatcatctt cttggtttac ttggtacaag ttctccttta gatcttcgaa acggttgatc
     5521 cgtgcttgct gcttggtgcc acgtgcctgt acgccagccc tcatccaagc taattcctgt
     5581 ttataaagcc gcttgcgctt ttcttcttgt tctacttcta cacgatcacg ttctgctttt
     5641 tctaagacgt aagcttcata atttccttta tactcgtata attttccaaa tgaaagttcg
     5701 aagattcggt ttgctactct atctagaaaa taacgatcgt gggtaaccat caatagtgcg
     5761 ccacggtatt gtttcaaata gttttccaac cactcgatcg cttcataatc taaatggttt
     5821 gttggttcgt ctagtaatag taaatcgggt tcatcaatca acacttgcgc cagactaact
     5881 cgttttttct gaccacctga taaagtactg atttttttat ctaattcact gattcctaat
     5941 ttttgcaaaa taatctttgc gttggtatct gtagtccaag catcttcttt attcatttgt
     6001 tcttctgctt tagcataacg tttttgcaca tcagggtctt ctccgttttc ccctagttcg
     6061 atcaatgctt cctcatatgc tcgaacagtt tggatcaagg ggctgtttcc ttgaaagact
     6121 gcttggagaa cagttaattc atcggaaaat tcagctgctt gagaaagata accaatacgg
     6181 taatcagttg gataaaacac tgtttgccga tcaccgtcgc tgctatcgat accggcaata
     6241 atatttaaga gacttgtttt acccgtcccg ttcgtcccaa tcaacccgat acggtctttt
     6301 tcgtggacca aaaaagaaat ctggtcaaac aaatcttttt caccataagt ttttttgagg
     6361 tcagttactt ttaattcttt caacatgttc cctccttatc ttggatattg tatcataaaa
     6421 atagtgaact tcaggaaaat ttttttggta tttgttcttt ttgtgttctc aaaaaaagaa
     6481 caggaccgat tcgatcctgt tctgatatgt tttgtttttt ataaaacttg aaaattttta
     6541 cctttttatt ttattggact cgtttttttt gactttgctc gaaacgcaaa tcggaaagaa
     6601 aagttatcag atggtttgtt catcgtataa gattctaaca aattgatatt cctgcgatag
     6661 ctgttcgcag aaaacgtcgt gattttttca gcaacttcgt attcatcaat gatttttctt
     6721 tcaagataat aacctcttag catttttttc gtaaacagac tatcttgctg gatcatccac
     6781 gaagaaatca aattccccga gttcattttt attccttcaa gtttgcgttg ctggataaaa
     6841 aattgaacca tcgtttcatc ataaatatca gtcaagttat ctaaactttc aataatcaac
     6901 tgagtatttt tttgatatgt ctctctgatt tcttggatat cttcatcact aataaatgtc
     6961 tgacgatcag catatcttct ttgccaaaac attttcggat ggagaacgat tcgcattaac
     7021 ctacggacaa caatgaacca aaagccgata aaagacaaaa tctggcttgt gatagaatgt
     7081 tcgaatcgag aaagaaaacg ggagtaaaaa cgataaccat tagaagaaag cttgcccgaa
     7141 cgataacttt catccaatcc atcccgttcg atcgacaaaa tcagtgcttg tatttcttgt
     7201 acttcttgct tttcatcatc ggttaaatct tccaagtaca gatcacgtat ccgttcttga
     7261 taattttcta tcactgcttc agtggctaaa aattcttttg tattacgatc aatttccttt
     7321 atttcttttc gcaaaacttc aacgacctct tctaaaatca tcaaagcatt caaatccgtg
     7381 cttttcgctt cctctgtttc cgtcagcata ggcaagacaa tgatccctat gaccaaagtc
     7441 actaagatga cacccgccgt cagaaacaaa agcaaagacc gctggtaaaa catcatatta
     7501 ttgattgaaa atggcaaaat aaaaatcgta gccaaactga ctgttccctt tacaccgcca
     7561 aatgttaaga tcaagatctc gttcaactgt tttttgaact ttttactgcc atctttgaat
     7621 acataaaaaa gactgataga aatgaatctg atcacaaaaa gcatgacaga gattaatacg
     7681 ataatcgcta ataaaagacc gtttggatat aaaccgtctc cccatactgg cgagaacact
     7741 tgcgtcaatt cgattcctaa aaatataaat actaatgcat tcagtgtaaa agcaatagtt
     7801 gtccatgtac tgtgggacaa gctcgacaat tctgcatcaa acacagagat tttcctaaaa
     7861 ccagaagctt ggagaatacc tgctgccact gcagcaatga ttccagatac atcaaatgcc
     7921 tctgccaata cataggctag aaaaggaagc aaaagttcga tcagcagata ccctgtgaca
     7981 tcttgtgctg atgctttttc gatcaaatga attatttttt gtttgaacca gacaagcaaa
     8041 aagccgatca aagctccccc aatactagaa actactagac tcatccccgc gtttactgct
     8101 gaaaaagatc cggtgatcag cgcaccaaga gcaaactgga aggcagtaac accagatgca
     8161 tcattcaaaa gaccttctcc ttccaaaata tgcatcgcct ttttagggat attcactcgt
     8221 ccagaaagag aactgactgc taccgcatct gtcggaccta acgctgctcc aaaagcaaag
     8281 caggcagcaa gtgggacact tggcaacagg aatgataacg tcgcacctac cccgacaagg
     8341 gtcaaaatga ctcctccaaa tgccaagaac aaaattgtcc cgaaattttt gagaatcgaa
     8401 gaaatatctg ctttttctcc ttctctaaat aacaatggtg caataatcat aactaagaac
     8461 aattcaggtt caaaatcgat tgattgtccc cactctgtca gacctaatac cactccaagg
     8521 aaaatttgga tcagtggagc tggaatcatt ggcaaaatgc ggctagctat gttcgataag
     8581 gtgatcgtta ctgcaaatac aataactaac tcagcaaatt ccatcgtcca tcccctttat
     8641 tttttatttt gtgtaagcat ttttaaaaat tgttcttttt tctttaaaat tttggtttcg
     8701 actacttcaa cctgtgtttg gttcccctct aattcacact gtacacgttt tgactccaaa
     8761 cgaagcagat catgtttcag acgttccaac tgacgataca tcaatatatt tcttttacgc
     8821 aacttacaaa gtatttgacg gcggtcatcc atcgaaaggg cgccatagcg atcatgcaac
     8881 agtttttttt gttgttcaaa actctctctc attttccaga tctcctatac acaaacttcc
     8941 atcattatag caaagttctt tttggatgaa aaatatttca ttgtttcatc gtttattgct
     9001 agttagaaat ttccataaat ttttcaacgc ctcattctat tccaaatagt atcagatatc
     9061 aagtaaaact taagaaagat aaaagtttct gacaaaagga tgtttacttt cccattcctg
     9121 cctttcttgt tcttttatca caacaaaaaa atggggctgc gacaaaaatc ttgtcacagt
     9181 cccttattct gaataaacgg tgtggaagag acagcctatc agtagtaata agacacatct
     9241 ctttcacagc ctcatttttg cgtaaaaatc gatttattcg aaaaattccc cgtatacatc
     9301 ttctacacgt tgatctggat tgatcacgat atacaagtta cctgtttttt cgctgatttt
     9361 tgttgattga tattgattgt ctaatccttc tgttccttta tctagatatg ggaagtacac
     9421 aagatcaggt gtgccatttc ggtaaaggat ttccattcgc tcgatatctt cgtatttcaa
     9481 tgcgcgatta aacatgccca tttccagtcc gcctaggttg atatcatgtg tcgcaactgc
     9541 gtccccttca tggaagattt cgattttaaa tccagcacac ggatggattt caacaaattc
     9601 actgccattg attcgtccaa aacttgttgt gatttgtttg atccaaagat cgccgatatg
     9661 acggcggttg atcgtccagg tttctccatt tctaaaataa aattttagcg ctgtcatttc
     9721 tttactcatt aaaaagactc ctttatgatg taatttattc ttgtactacc tcagtttagc
     9781 acaaatttat gtaagtggtc acatcttatg ttttttcttc taatgacgta tctgcccact
     9841 ttaagtagcc aacaaagctg agcagataca gtctagtgtt ttttaaatca atttttcttt
     9901 cgataaaaaa tccaaaacct gttttttatc attttttatt ctttgttcta cgacggcttt
     9961 ttcagctaat tgcaaagctt cactaaccca cttaccaggt ttttttccag tttcttctag
    10021 caagatttta ccatctactg ctaattcgct taatgacttg ataggtaaac gatcatagga
    10081 ttctcctacg gcatccatct gaggtgtttc gtcaaaatag atcatcaatt cttctacttg
    10141 cagtgcatgt tttttgccta agaagtacaa ttcgaatggt tccaaagctc cagtttctcg
    10201 ttgtcgtagt ccttggacaa gtgcttgaac gtctttaatc atttggttag aacacttcca
    10261 cgatttcaaa aaggaacgaa tctcattttc ttttagatcc agttggctga tcaataatgc
    10321 ccatgcttgg ctctcttctt tcaatgggac ggatggtaat tcagcaaaac gtaaaagtgc
    10381 ttcgccttct tctttcaaac cgggacagca aatgtagcac tctgtttcaa taaatgcatg
    10441 gattcccgcg gcccgttcat ttcccaagag catcttgatg aattccacat ttatacgttc
    10501 tacagatatt ttacctaata gctcgtggtt ttctttgatg gcgtagaagg tttcttcctc
    10561 taatttgaac cctagttgac tgacaaaacg caacccgcgc atcattcgta aggcatcttc
    10621 atggaaccgc tcatgaggat tgcctacagc tcgcaagatc tgttcttcta gatcttgcag
    10681 accatcgaac aaatcgatga tttctccatc ttctttcaaa gcaaacgcat tgatcgtaaa
    10741 atctcttctt ttcaaatctt ctgccaacga acgaacgaat tcgacatgat ctggtctacg
    10801 gtagtcctga taagttgatt ccgtccgaaa tgtggtgatc tcatattgtt catcattttc
    10861 taataccaac acagttccat gttcgatccc aatatctatc gtttttggaa aaagctgttt
    10921 gatttcttct ggaaatgcgc ttgttgcgat atccacatca tggattggct ggttcagcaa
    10981 gatatcacga acacttccac ctacaaaata agcttcatat cctgcattct ctaatttttt
    11041 cagaactgga attgcctttt ggtattccag aggtaattgt tttaatttca tttttccaac
    11101 ctttcctgcg tgtttcttgt cctgagataa agactcagtt gcatggtgac tgtttgacaa
    11161 gtccgtttca gagtcttcaa ggccaggacc acttcatctt gtttactcgt ccttacgctc
    11221 aaagcgatta tggtctcttt gattgaattt ttccatgttt ttttcaaaaa cttcagttaa
    11281 gtcgatattc aatgaattag ccatgatcat cgtaacgaat aaaacatcac caagttcttc
    11341 tgctaccgag ttaactggtt cagaagcctt tttgcttttt tctccataag tgtggttgac
    11401 ttctcttgcc aattcaccta cttcttcagt taatcgagcc atctgagcta atggagagaa
    11461 ataccccacc ttgaattgct ggatatattc atccacttcc tgctgcatac tgtataaaga
    11521 acgtttttta tccatctttt tccctccaaa actatcttac caaaacttta acgcaaatcc
    11581 tagataagag caattgagaa aatcgaattt ccatgttaca tttaggaagg aatttctaaa
    11641 tggaaattct gtgaaaatac ataattgaaa gctggaattc atatggtcaa acgaaaaaca
    11701 gtaaaagaag ttctttttat cattatagga acaagtattt atgcatttgg tcttgtttac
    11761 ttgaacatcg ctaatcattt agcagaaggg ggagtctcag ggatcacctt gatcttaaga
    11821 gctttatttg gtattgatcc agcttatacg actttattaa tcaacatccc attgatttta
    11881 ctaggaggaa agatcctcgg taaacgttcc ttggcctata ctgttctagg tacactttct
    11941 ttgtcattct ttttatggat ctggcaaagg gttccactag aaatcaattt acagcatgat
    12001 ctactgatcg tttctctact tgcgggttta gttgcaggtg taggaagcgg gatcgtctac
    12061 cgcatgggtg gaacaacagg cggcagtgat attatcgctc ggattttaga acaaaattac
    12121 ggcatcacta tggggcgttc cctattggca tttgatatca tcgtcttact tgcttcatta
    12181 acctatatcg acttgaaacg tatgatgtat acgttgattg tttcgtatgt tttcagtcgt
    12241 gtgattgatt cgatcttaga tggcggatat tctgcaaaag gtctacttgt cgtctccaac
    12301 aaaagcgaag agattgcacc actactgatg accggtctgc aaagaggtgt gactttcctt
    12361 tctggagaag gcgcattctc tggtaattca aaaaaaatca tctatatggt cgttagctct
    12421 agtgaattaa ctgaagctaa gaggatcgtc cacgagattg acgaaaaagc tttcctttca
    12481 atcatcaatg tgcatgaagt ggaagggcaa ggctttactt atttgaagcc acagacaaga
    12541 ttactgaaaa aactataaga aaaaagacaa agataggcta tgaggaaaga ttccacatcc
    12601 agctgacctt tgtctttttt agtactatac tatttttaac taattgttat gttcacttac
    12661 ccggttactt ttcttttgtg tctgcaaaag gatctttcgg ttaacttcat cagataattt
    12721 caaaaaagca tccctatctc catcttctag tgcttgatcg atttgagcat aaagcatttt
    12781 tagttttgcc tcttcttctt tttcttgaaa atatcgttca attaattcag tatcttcttc
    12841 actgactgaa gaatcccaag aagaaaatgg attgtcttct aagatagata gatattctct
    12901 tgtctgccaa gcattttcga atatacattc cacatataaa ggctttttcc aattcaaacg
    12961 gatttcatga aaaatctggt ctgtgtccgt aaattctttt ccctgcaaaa acaatttcat
    13021 gggttcccct tcaacagata gatcactgat ctgcagtcct cgatccgcaa aattcgcccc
    13081 ttcaacaaag tggacattgc ttaaaatcgc ttcatgattg gcaagataat tcaagatcca
    13141 aaaaacttct cttcggctaa aagatacatg attgaccatc cacgttaaaa aattcttctt
    13201 ttcccgtaca tcaattagca tttttctcac ctctctgtta ggtcatctaa taatgacagt
    13261 gcctccatat ctcctggttc aagtgttaaa taataagtca ataattcttg cgctcttttc
    13321 agctgccctt cttctcttaa gaaaaaggca tactctttca agaaatctgg ttcatgtgat
    13381 agttcatgat aagcctgttc atagtgtaca gctgcaacgg taaagtcttc taattcatta
    13441 taagcatggg ctaggttcca ttctgcataa ggattagcag tttcttccat ctgattgatc
    13501 acttgtatca catcttcata tctttcatca tccaagtaaa gattacttaa agtaagtaat
    13561 gtttcatctt gtttgtctcc tacttctaaa gctttcagta aaagttcttc tgctcgctgt
    13621 ctatcatgca gacggaaagc attttccgag gccaggtgat aaagttcgac ttggtaagga
    13681 ttttctttga ttccttcttc taacactgtt tgtgcttctt caatcatttc ttcttcttga
    13741 agagcttgtc ctagatatag atataacgat tgataatgcg gattaacttc tcgaagttct
    13801 tccagatagt tgattgcctg ctgattttct ttcaattgaa gattcgtaaa agctagctgg
    13861 aacagtcgat catctgtcct ttcttctttc agtgcttctt ctaaaggtac gatagctgct
    13921 tcgaattctc cttgcatact taaggattgt cctaaacgtt cctgcatcga gattcctgaa
    13981 atttcattta tcccgcttgc caacaatgtt tcataaacca ctgctgcctc tttgaaacga
    14041 tcaactgaaa aatacaattc agctagcgca aactggatca atggttcatc aggaagcaac
    14101 ccagctgctt ctttcaattt tgcttcactc acttcaggga ttccgataac ttggtacaaa
    14161 tcagctatcg ccaataagct ttgcggatag taatcacttt gcttatccac tttttccagg
    14221 tattcaaaag ctaagtcgat ctcattatct tcgattgcaa tttctgctaa cgggatgttc
    14281 agtccgtcat tttctgggta ttggttgatc agttgttcaa aaatctgttt tgcttcgatc
    14341 aaaaagccaa tagccagcaa ttcttcgcct agttcagcta atatatctgc tgggtcttct
    14401 tttaatgctt tttctaaaaa gatttgtgct tgtgacaagt cttcatctga taaagcttgc
    14461 aacatttttt cgctatttgt attcatttca attccctctt atctcactga cgatttgttg
    14521 aaataccgtc attgttctca ataattcttt ttcgtttgga tgttggtatc cgtctattat
    14581 acctattttt tctaaaacaa gtattttttt gcttttttct gccaaaaaaa gaactttttc
    14641 caaatactcc atctttgaaa tctgttccag ccagtggatt ggatagccaa gcaagtccag
    14701 ccacacaaaa aagtttttta gttggaaatc aaaaggatat gcttggagat tccacacgaa
    14761 atgaaacatc attcctaaaa attttttcat actagcagaa agtagatggc cattttcggt
    14821 cagataaaaa gcttgttcaa acagtttacc ataatttgac aggttttctg cctctaattg
    14881 ataaaaatag atcatttctt cgatcatccc ggcaaacggc cgagtcgtta attgcttttt
    14941 atttggatag tttttataca gattttgcaa aaaacgatga tcacaaatca ttccgcaaca
    15001 gataaaagtc aaaaagtcaa tctgctttcc gtccacctgc cgctcgctga ttgtttgatc
    15061 ataaacgatg cgttctggca gattgatggt ttgcagcact acacgatttg gtagctgcaa
    15121 aatcgtccgc tgcggtgtta atgctcggct tagagaacgg atcgatacag ggatcatcca
    15181 tagtgatgaa tcaagggttg tatgtttttg aaaaaaacct gccagatcca tgatcccttc
    15241 atttccaaat ccgatgataa acatggattg attttcggga tagcgtttga cgaaatcaag
    15301 caggttcttt aattctgtta aatgattgca ttgagtgttg gcacaaatat accagtcatt
    15361 gtttgcttga tttgcaaata acgggttgat cttatctgca aacaaatcat aatagcgctg
    15421 attcgttaaa aataagattg gctgatcaat ggaaagttct cttagctgac tgactagcgt
    15481 ttctccgtaa aacactttcg tcaacgcatc ttctacttga taatcaagtt ccatacgcat
    15541 ccctcctctt cttattttta ttttaccatt agtaagcaaa ttctagaaaa gataagagca
    15601 aagctcacct cataacttgc aaataaataa aaattaaata aaatacatgt gttttctatg
    15661 tatgacatac ggattcggaa tctctgtctc ttatttattc cttttctgct aggaaataaa
    15721 aagaaaagca cttgtagccc tagagccaca agcgcttttt caactgtctt atttaacagc
    15781 gtcttttaat gctttacctg gtttgaatgc aggtactttg ctagctggaa tttcgatttc
    15841 ttcaccagtt tgtgggttac gacctttgcg agctgcgcgt tcgcgaactt caaagttacc
    15901 aaaaccgatt aattgaactt tttcgccgtt agctaaagca tcttgaatag ttgaaaatac
    15961 agcatctact gctgcagttg catctttttt agttaaatca gtagctgaag caactttttc
    16021 gattaattct gctttgtttg ccatggatag attcacctcc tccacaagag atatgataga
    16081 aatatcattt agttaaaact ttctgagtgg tccagaagtt taaaaagaaa tattgattaa
    16141 cttcaatatt ctgttatcac gatatcatat gaaccgctta atagcaaggt ttttaccaat
    16201 tttcacatgc tttttttagt catagaaacg taacaaacga aactgtcttt ttcttttgaa
    16261 aaaaaaacgt cgatatggta aaatgcctat tttctcctac gagggataat tcggatggac
    16321 gtcccttcaa aagtaaatgc ttttctgatt tgattttcta agaaacgttc ataagaaaaa
    16381 tgcatcaatt cctcttcatt gacaaaaacg acaaatgtag gtggtttgac agcgacttgt
    16441 gtagcataaa aaatcttcag gcgttttcct ttatctgttg gtgttggatt gattgctaca
    16501 gcatccataa tgatatcatt caacacagcc gaagagatac gaagattctg gttcatactt
    16561 acttgttcga tcatttcagg cagacgttcc aaccgttgtt tagtcactgc agaaacaaaa
    16621 acgattggag cgtaatcaag ataccggaat tcatcacgaa tctcttgttc gaaatcacgc
    16681 attgtattgg tttctttctt tacaagatcc catttattca cgacgataat aattccacga
    16741 ccagcttcat gggcgtaacc cgctactcgc ttgtcttgtt cccgaatccc ttcttcagca
    16801 ttcagtacca tcaaaacgat atcagaacgg tcaatggcac gcattgcacg catcacgctg
    16861 tatttttcag tattttcgta tacttttccg cgtttacgca taccagctgt atctatcatt
    16921 aagaatttct gaccactttc agaaacgaag tgggtgtcga ttgcgtctcg ggtcgttcct
    16981 tcaatatccg aaacaattac acgttcttct cctaaaatcg cattgatcaa agaggattta
    17041 ccaacatttg ggcgaccaat caaactaaac ttaatcgtgt cgtcgtcctc ttcttcagtc
    17101 tcttcagaga aatacttcac tgcttcatct aaaatatcac caatccctag tccatggctt
    17161 cctgaaacag gataaggatc gcctagccct aaggaataaa attcatagat atcgtttctc
    17221 atttctggat tatctacttt attgactgct aaaatcactg gtttattact tctgtataaa
    17281 attttcgcaa ccatctcatc tgcgtctgtc acgccttcgc gtccgctgac tacacaaatg
    17341 attacatctg cttcttcaat cgcgatttct gcttgatgct tgatctgatc cataaaaggt
    17401 tcatcgccaa gatcaattcc gcctgtgtcg atcacgctga attctcgacc taaccattca
    17461 ccttttgcat aaatacgatc acgagttact ccaggagtat cttcgacaat cgagatacgc
    17521 tcaccagcga tgcggttaaa aatcgtagac ttaccgacgt tcgggcggcc aacgattgca
    17581 attgttggat ttgccatatt atttcctcct cacaagtttc caatctcact tagtttaacg
    17641 aatgcattgc caactttcaa gcatagattg tcaattaaac gaagtatttt tattccttat
    17701 tgaaatacta atcgcttttc aaaagaaaaa ctgagacaaa agcagctgct ttcatctcag
    17761 tcacagatac aaaatcaatt atttttcaga atcatcggtt gctttttctt ctacttcttc
    17821 tttcaacgca tcacctaaaa tgtctcccat agtaaatcca gtattttctt ctggtaattc
    17881 ataagactca acattttttg gttcttcttc tgaagctggt ttttcttcta aagctttgat
    17941 actcaatgca atacgatgtt cttctggatg gatctctaat actttcactt tcacttcgtc
    18001 accttcatga agcacttcat gtggtgtcgc aatatgttta tgtgaaattt gagagatatg
    18061 gactagccct tctactccag ggaacacttc aacaaaggca ccgaagcttg tcaaacgttt
    18121 aactgttccg tctaatactg tgccaactgg agcttttgta tcgatttctg accaaggtcc
    18181 aggctgtgtg tctttgattg acaaggaaac acgttcttgt tcaggattga tagacaacac
    18241 tttgacattt acatcatcat tgacagccaa tacatcactt ggttttgcta catgactatg
    18301 tgaaatctct gagacatgga ctagaccgtc aattccgcct aggtcgacaa atgcgccaaa
    18361 atcagtaaga cgtgcaacac gtccactgag aacatctccc tcatggatag ttgaaagaat
    18421 ttcttttttc ttttcttctc tttcgttttc cactaccgct ttatgcgata agatcaaacg
    18481 attttctgat ggttcgattt cgatgatttt aaagctcaat gttttgcctt tatagtctga
    18541 aaagtctgca acaaaatgat cttccaccat tgatgcaggg acaaaaccac gtacaccgac
    18601 gtcaacgact aaaccgcctt tgacaacatt tgtaacaggt gcttcgatga ccttgccctc
    18661 ttggaaatct ctttcgatat cttcccaaac ttttttagca tctaaacggc gttttgaaag
    18721 tagatagctg ccgttttctt tatctttgcc gatcgtactg ataacgacta aatctaatac
    18781 atcaccgact tttactactt cgctgatatc ttctaccggc aaagttgata attcttttgc
    18841 cggaacgact ccttcaactc cagccccttc gattccgaca atcacttgtt tgtcttcaat
    18901 gactaaaact tcgcccttaa caacatcgcc aacttttact tcttgtacgc ttttcattgc
    18961 gtcttccatt gactggttgt tctcttgatt ttgatcaaat tctgtcataa cacttgtcct
    19021 cctaaccaac attctgccgt aagtacggta ctgtttttag tttaattgat tattcaaaaa
    19081 aaataaagcg atttcgttcg ttttccttga aaaaacaatt gtttttcatt ttttttctta
    19141 atttaaaaaa gaagataacc ttttttcaaa acaacagctt ttattgctgc aacgacttct
    19201 ggaatcgatt tgccagtcgt atcgatacga acagcatcct ctgcttgctt cagcggagag
    19261 acttcccttg ttgaatcata atggtctctt tcctgtatct ctttcgtcag tgtttcaaga
    19321 tcagtcggaa ttcctttttc ttggttctct ttgaaacgtc tttctgcacg ctcacttacg
    19381 cttgctacta aaaagatttt cacttctgca ttcggtaata cagctgtccc gatgtctctt
    19441 ccgtccatga caacgccacc gcgttcccct atttttcgtt ggacagcaac taattcttct
    19501 ctgacttttc catgtgcaga gacttcagaa acagcttttg tgacatctgg ttgacggatt
    19561 tctaaagtca catcttttga tgctatgaat acatgctgtc catcttcttg ttgcgaaaaa
    19621 gagataggat actttttgat cagcgaaact agcccttctt catctgaaaa agatacattg
    19681 ttcgtaatag ctaaataagt tactgcgcga tacatggcac cggtatctgt ataaatataa
    19741 ttcaattctt ttgctaatat cttggcaacg gtacttttcc cagatgaagc aggcccgtcg
    19801 attgcaatac tgatttgtgt catgatataa ctcctttttc tcagaaaaaa agccgcaaag
    19861 cgcggctatt attatttaat tcttaattct tgaccagggt aaagcataaa gttgtttgga
    19921 tctaagccat tcaattcaaa caattgatca gtggtgatcc ctgcacgctc agccacttgt
    19981 tttggacctt cgcctgcttg aacagtcgtt gttgaaccgg ctgcagctga tgaagaaggt
    20041 gttgtttctg ctgcattgct gcttgaatcc cctgcttgat aagttgcctc acttgaggaa
    20101 gtcggtgttg tctctgctgc aatactgctt tctgacgtta cacctgtatc ttgagacaca
    20161 gtagattctt ccgcgattgt actagattct actgttgaag tctcagttga ctcctctttt
    20221 gttgtacttg tagaactttc tactactgat gtcgaagttt cagaagaagt tgctgctgtt
    20281 gcattgttgc ttgatccatt tctgatccag aaataagcac ctgctggaat agcgatacat
    20341 aagaccagta aaatcaaaaa tagcgaaaga aataatgtat ttcctttctt ttgttgtctc
    20401 tgagaacttc ttgatgaaac ttcatcatct gtatcgtaga ttggttgttc ccatggttct
    20461 tgtgtttcgt tattttctgt agattgatgt ctatcttttc tgctcactgt tttacctcct
    20521 aaactctatc gtgtgtatta taccatagga aaaagtgtct tgtcctaaaa aatcaatgtt
    20581 ccatgttttt tttaaataaa tttagtaaaa tcgtttccca atgaagttgt tcagtcttag
    20641 aaaaaagctt cctctttggc agaagttctg cttttgctcc atcaatatca caacaattct
    20701 gcggcttttc ggagagtttt tcgccaaaat aagctaagat gaattttctg cggcagtttt
    20761 cttcattaat atagcgaagc atctgctgca ggcggaattc tttttctttt tcctgacgtt
    20821 tgagccgttc cacccaattt tctggttttt cagactgctg gatcagctcc atccattttt
    20881 tttgcagctc atcaaaggga gcttgctctg tggcatattc caaataaagc tcaaaacttt
    20941 gtcgctgttc tctagaaagt tgattaaaga aataatggat acgttcatcc ccactttgat
    21001 aaaaaagaat tgctgcactt tcttcctgat ctctgcctgc tcgaccgatt tcttgtacat
    21061 agttttctag actgtctggc aaatcataat ggaccacata acggatatca gatttatcga
    21121 tccccatacc aaatgcattc gtagcaatga gaaactgcag ctgatttttt acaaactgct
    21181 gttgaagttg tcttctttga gctgcatcaa gaccaccgtg atagtatcca actgtaaaac
    21241 gttccctgaa taaatgatac aatcgttcga cttctttttt tgttgcacaa taaatgattg
    21301 ccgctccttg tgctctttcc atgaagtgtt caagttcttg ttctttttgc tgcgttttcc
    21361 gaacaaaaag cgagatattt tgccgattga ctgattgtct gatctctttt ggtggtcgat
    21421 caaatagtac atgacgtata tctcttgcta ctaaatctgt tgctgtcgcc gttagtgcca
    21481 gtgtgaccgg gtttcctaaa agctttcgga tttttcctaa ctgttgatat tctggacgga
    21541 aatcaactcc ccattgagac acgcaatgag cctcatctac tacatataac gcaatatttt
    21601 gttttttcag atgctctaaa agagaaggat tagtcaacat ttcgggactc aaaaacaaaa
    21661 atttatattg cgatagatga cggaggacgt atcttctttc agattgcgaa agtaaactat
    21721 tgaaagcagc tactcgtttc tcccctcgtt tttgtaattg cgtcacttga tcttccatta
    21781 gagaaagcag cggtgacacg atgatgacca gaccttccat taaatacccc gtcaattgat
    21841 agcaaaggct ttttcccgtt cctgtaggca aaatagcgag agtatcttct ccatcaagca
    21901 acgcctgaat aatttcttct tggccgggac gaaatgaatc aaatccaaaa tatttttttt
    21961 aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg
    22021 aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac
    22081 ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg
    22141 aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa
    22201 atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat
    22261 catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga
    22321 atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata
    22381 aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt
    22441 tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg
    22501 caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag
    22561 ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat
    22621 acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc
    22681 ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa
    22741 catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg
    22801 ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt
    22861 atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc
    22921 cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa
    22981 aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt
    23041 aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt
    23101 ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag
    23161 ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga
    23221 gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt
    23281 tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat
    23341 tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg
    23401 acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt
    23461 tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca
    23521 aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc
    23581 ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa
    23641 acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat
    23701 acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca
    23761 cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc
    23821 gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag
    23881 ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca
    23941 ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag
    24001 taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac
    24061 gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag
    24121 ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc
    24181 tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca
    24241 gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt
    24301 tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac
    24361 tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg
    24421 caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg
    24481 gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac
    24541 tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt
    24601 tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc
    24661 tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg
    24721 ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt
    24781 cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa
    24841 ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac
    24901 caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt
    24961 cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg
    25021 ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata
    25081 agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc
    25141 cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa
    25201 agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat
    25261 acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt
    25321 cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa
    25381 cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt
    25441 aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt
    25501 tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat
    25561 ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt
    25621 gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca
    25681 aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc
    25741 aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac
    25801 ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt
    25861 acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac
    25921 aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc
    25981 aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga
    26041 tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg
    26101 tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat
    26161 agggatatag aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc
    26221 ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat
    26281 accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa
    26341 ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac
    26401 taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc
    26461 tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt
    26521 gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat
    26581 aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc
    26641 ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg
    26701 atctttttcg atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat
    26761 aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga
    26821 agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac
    26881 taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag
    26941 taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa
    27001 taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga
    27061 agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac
    27121 caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg
    27181 tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg
    27241 ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc
    27301 agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata
    27361 cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt
    27421 tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa
    27481 aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct
    27541 cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta
    27601 taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg
    27661 atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg
    27721 catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt
    27781 tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga
    27841 agttgcgcat gcagcattaa aaagacagca ctcacgatga atcctttgat caagttgaaa
    27901 ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc
    27961 agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa
    28021 atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt
    28081 ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta
    28141 gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa
    28201 cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc
    28261 gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa
    28321 tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg
    28381 gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc
    28441 tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc
    28501 gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc
    28561 gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac
    28621 cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat
    28681 tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca
    28741 aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag
    28801 tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca
    28861 tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg
    28921 tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct
    28981 ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa
    29041 cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat
    29101 ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt
    29161 cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag
    29221 aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga
    29281 cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt
    29341 gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga
    29401 tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat
    29461 tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt
    29521 atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc
    29581 atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc
    29641 agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct
    29701 tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt
    29761 gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa
    29821 tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga
    29881 ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct
    29941 taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact
    30001 taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt
    30061 ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag
    30121 tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat
    30181 ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg
    30241 tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac
    30301 ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc
    30361 catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca
    30421 gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc
    30481 gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg
    30541 tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta
    30601 gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg
    30661 gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac
    30721 cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt
    30781 ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc
    30841 cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga
    30901 cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat
    30961 aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc
    31021 agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa
    31081 actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc
    31141 accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt
    31201 cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac
    31261 tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg
    31321 tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat
    31381 tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc
    31441 ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc
    31501 acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga
    31561 aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata
    31621 catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc
    31681 aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg
    31741 cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg
    31801 gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg
    31861 caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt
    31921 ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt
    31981 ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat
    32041 aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt
    32101 ccataatctg a
//
LOCUS       DACCWV010000016        31627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCWV010000016 DACCWV010000000
VERSION     DACCWV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.016"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..819)
                     /locus_tag="IVW84_000681"
     CDS             complement(<1..819)
                     /locus_tag="IVW84_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7916901.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPF"
     gene            complement(961..1140)
                     /locus_tag="IVW84_000682"
                     /pseudo
     CDS             complement(961..1140)
                     /locus_tag="IVW84_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(1343..1702)
                     /locus_tag="IVW84_000683"
     CDS             complement(1343..1702)
                     /locus_tag="IVW84_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7916902.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(1732..2073)
                     /locus_tag="IVW84_000684"
     CDS             complement(1732..2073)
                     /locus_tag="IVW84_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916903.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(2070..2744)
                     /locus_tag="IVW84_000685"
     CDS             complement(2070..2744)
                     /locus_tag="IVW84_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7916904.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(3921..5219)
                     /gene="asnS"
                     /locus_tag="IVW84_000686"
     CDS             complement(3921..5219)
                     /gene="asnS"
                     /locus_tag="IVW84_000686"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7916905.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(5259..6449)
                     /locus_tag="IVW84_000687"
     CDS             complement(5259..6449)
                     /locus_tag="IVW84_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7916906.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(6470..6997)
                     /locus_tag="IVW84_000688"
     CDS             complement(6470..6997)
                     /locus_tag="IVW84_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7916907.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(7044..9833)
                     /locus_tag="IVW84_000689"
     CDS             complement(7044..9833)
                     /locus_tag="IVW84_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7916908.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9980..10180)
                     /locus_tag="IVW84_000690"
     CDS             complement(9980..10180)
                     /locus_tag="IVW84_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7916909.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(10633..10953)
                     /locus_tag="IVW84_000691"
     CDS             complement(10633..10953)
                     /locus_tag="IVW84_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916910.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(11456..12574)
                     /locus_tag="IVW84_000692"
     CDS             complement(11456..12574)
                     /locus_tag="IVW84_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916911.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            13045..14352
                     /locus_tag="IVW84_000693"
     CDS             13045..14352
                     /locus_tag="IVW84_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7916912.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            14473..15672
                     /locus_tag="IVW84_000694"
     CDS             14473..15672
                     /locus_tag="IVW84_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7916913.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(15699..16967)
                     /locus_tag="IVW84_000695"
     CDS             complement(15699..16967)
                     /locus_tag="IVW84_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7916914.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(17137..17778)
                     /locus_tag="IVW84_000696"
     CDS             complement(17137..17778)
                     /locus_tag="IVW84_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7916915.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(18187..18690)
                     /locus_tag="IVW84_000697"
     CDS             complement(18187..18690)
                     /locus_tag="IVW84_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7916916.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(18813..19577)
                     /locus_tag="IVW84_000698"
     CDS             complement(18813..19577)
                     /locus_tag="IVW84_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7916917.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            19685..19960
                     /locus_tag="IVW84_000699"
     CDS             19685..19960
                     /locus_tag="IVW84_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7916918.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            20520..21470
                     /gene="yidC"
                     /locus_tag="IVW84_000700"
     CDS             20520..21470
                     /gene="yidC"
                     /locus_tag="IVW84_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7916919.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(21516..21917)
                     /locus_tag="IVW84_000701"
     CDS             complement(21516..21917)
                     /locus_tag="IVW84_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7916920.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(21931..22740)
                     /locus_tag="IVW84_000702"
     CDS             complement(21931..22740)
                     /locus_tag="IVW84_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7916921.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(22727..23617)
                     /locus_tag="IVW84_000703"
     CDS             complement(22727..23617)
                     /locus_tag="IVW84_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7916922.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(23630..24109)
                     /locus_tag="IVW84_000704"
                     /pseudo
     CDS             complement(23630..24109)
                     /locus_tag="IVW84_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(24285..25226)
                     /locus_tag="IVW84_000705"
     CDS             complement(24285..25226)
                     /locus_tag="IVW84_000705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP7916923.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(25223..26218)
                     /gene="lacD"
                     /locus_tag="IVW84_000706"
     CDS             complement(25223..26218)
                     /gene="lacD"
                     /locus_tag="IVW84_000706"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7916924.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(26211..27407)
                     /locus_tag="IVW84_000707"
     CDS             complement(26211..27407)
                     /locus_tag="IVW84_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7916925.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(27425..28153)
                     /locus_tag="IVW84_000708"
     CDS             complement(27425..28153)
                     /locus_tag="IVW84_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7916926.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(28552..29721)
                     /locus_tag="IVW84_000709"
     CDS             complement(28552..29721)
                     /locus_tag="IVW84_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7916927.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            complement(30741..31232)
                     /locus_tag="IVW84_000710"
     CDS             complement(30741..31232)
                     /locus_tag="IVW84_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916928.1"
                     /translation="MSPINYRAKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDAL
                     KYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGK
                     KSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEM
                     KID"
     gene            31345..>31627
                     /locus_tag="IVW84_000711"
     CDS             31345..>31627
                     /locus_tag="IVW84_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7916929.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKN"
BASE COUNT         9331 a         6595 c         5371 g        10330 t
ORIGIN      
        1 aaatggttta tttttattga taaatccact taatactgtt tcaccaacat atacagattt
       61 agggactgat tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt
      121 tccatctaac ggtacacgtt caccaggctt cacaagtaca aactgaccga tctgcacgtc
      181 ttcagggttc acggtcatca atccttccgt cgtctgcacg tgagctgttt ctggacggat
      241 cgccatcaat tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc
      301 gccgatttga taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc
      361 gccaatcgta gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt
      421 catcactgcc ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat
      481 caagcgaatt ggttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa
      541 taaaatccgg aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt
      601 atctatttct gttactttga catctggttc taattccttc actacttttt ttatttcttg
      661 ttcaatcgta gccaaatgat cttcctctgc gtcaatcgtt agttttccag aagtgaagtt
      721 taccgttgct tccttgactc cattgatctt ctgaactcct ttttcgattt tcattgcaca
      781 gttcgcacaa tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca
      841 acatatgaat atctcttcat aactaattat aaatgaacgg ttattcatat gtcaaggtat
      901 ccgaaagaaa aaatgaaaaa catctgcatc ggaaaatttc ttccatacag atgttttggc
      961 ttattctttc tttttctttt cgatcattgc cactgctctt atccaacccg cacttgcctt
     1021 cttgatcttg acaggatagc aactaagttt gaagcctgtt gctggcaatt tgtctaaatt
     1081 cattaatttt tcgataggac aatatctttt ttcaatccct gcaaaatgtc cttcccagat
     1141 attcgaagct ccactacgct ttcaaaaata tagaaagagt tctttaatga aaattatttc
     1201 aactttccat aaacagtaac cccatataaa taaaagacgt aaaagtcaca tggaagaact
     1261 tatacaggag aacgcataga gtcaggtgaa atacatgtat cccacctgac tctatgcttt
     1321 atctaacctt tattattctc ttttatacaa tttctgcacc gaatggcatc aacgccaatc
     1381 gagcaagttt gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga
     1441 tgccgctgac gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat
     1501 taacaatcaa tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag
     1561 aaagggaggc gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc
     1621 aaagtatccc cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga
     1681 tgacatttcc taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg
     1741 ggcaaaaaat gattttattc ttcttttttt agcttccagt atcaaatatt ctgcaaattt
     1801 tttttggatt tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga
     1861 tggtcgctga tccaatcgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat
     1921 ccgtttttct tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc
     1981 cgtttttgct tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtag
     2041 atcttctttt actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta
     2101 tctccactaa aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca
     2161 tgtccgccaa tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataaaca
     2221 atccctaaag cattgaatat accatctaaa ggaaccagca atgcgccata ttccttagca
     2281 agtttacgaa caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg
     2341 gttataggaa gtacaaacgg ctccatcaag atgatgttgc agattcccac tgtttgaagt
     2401 tcatttagta attttcgata aacggattca aattgctgag cagatttctc agttccgaat
     2461 atagcctgat cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag
     2521 tcaggttgta aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct
     2581 cctcctatcc cacggttcat gcattcaaat tccatcgttg gatattggga gattagtttt
     2641 ccggcaatca aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct
     2701 gcgtctgtaa tgctatcacc gataaataat atacgttttt tcatttgtca aaaactcctt
     2761 tttgaataaa acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag
     2821 agaaaatata tgtataaaaa atgacaccag cctagtccct tcaaacaatt gaatgaacgc
     2881 tcatcaagtg tctagttttt tatgctattt ttttcttaac gtgccggatt ccccttgatt
     2941 ccttcgttgt ttggtccaga gagatcgatc acacctaagc ttccgtcaat cagaccactg
     3001 atgacgccat cagaacatat atttataggc tcatctaaga cttctagtaa atgtcccttt
     3061 actaatatct cttctagatt gcttgggata gattggtcat aactttatct ggtccgatga
     3121 tttcaagggt gaattgaact tacatatgga ttattgcatg ctgaaaatac ggacctactc
     3181 catcttcgaa tgttactatt agtttgtttt caggtgctag atgctgcttt atttcaacag
     3241 cagaagcatc atcaaatcag atttccattt gaattacctt ctttgtaaat gtttgattga
     3301 ttgcgatttc gttatagaac cttgtgaatc aaatagcaat ttcttttgct ttcttctctt
     3361 gtctgctgga aacttccttt atcccgtatt ttaaaaaaag aactgcgaga gttcgcagtt
     3421 cttttttaga ataaacggtg ctctccggcc aactcctcgg tcacaagtca caatagtcat
     3481 caaacatgaa aaactgtttt ttgaaattac tccttgtgac tcggagctga acggcaggcg
     3541 cacaacctct tgatataaac ggtgttcaat cagctgaccc tcgatattag ctaaaaaaac
     3601 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atactcaggt caaggcactg
     3661 atttcacaac ctcttcctta aacggtgttc aaaagccagt ttctcggtat taagccaaat
     3721 ttatgcaaaa atgtggaaag ccatttccac aaaatttttc ttaatactcg aaactaaaca
     3781 gctttttcac aacctctcca ctaaacggta ttcaaaagct tacatctgcg gtaataagtc
     3841 ccgccaattc aaaaacatga acagctgttt ttgcttggct gttctaatta cttgatgcag
     3901 aacagctttt tcatcacctc ttatggataa attcggttca acaaacgtgg gaatggaata
     3961 gcttcacgca catggtcgat accagcaatc caagtgactg ttcgttcaag gcctagacca
     4021 aaaccagagt gcggaacaga accgtattta cgtagatcaa gataccatga atattctttt
     4081 tcatccaaac cgtgattacg gatttgttct agcaagaaat catagtccgt tgcccgttcg
     4141 cttccgccaa taatttcacc gtaaccttct ggtgcgatca tatctgcaca aattaccacg
     4201 tcttctcttg ttggatgtgg tttcatgtag aatggcttga tagcttttgg atagtttagg
     4261 ataaagacag gctgttcgaa tgaattcgca atgaacgttt catgaggtga accaaagtct
     4321 tcgccccatt cgatgtcatc aaatccattt ttcttcagta attcgatcgc atcatcataa
     4381 gtaatacgag ggaatggcaa ttttgtataa gtttttaaga cttctttgtc tcgtcctagt
     4441 acatctaatg cgtattcgca atgatctaaa acattttgta ctaagaatgc tacatattgt
     4501 tcctgtactt ctaaactttc ttcttgatga gtgaaagcca tttctggttc gatcatccag
     4561 aattcaatca aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa
     4621 aagacttttc cgaaagccat agcagcagct tccatgtata attggccact ttgagataaa
     4681 taagcttttt gatcaaagta atttgtttcg aatagatccg tagtgccttc tggtgcggaa
     4741 ccagtcaaga tcggcggatc gattttgaca aaatgatgtt gattaaagaa ttcatacgtt
     4801 gcacgaatga tttcatttct gatctgcata atcgcatgtt gtcttgaaga acgtagccat
     4861 aaatgacgat gatccatcaa gaagtctgtt ccgtgttctt ttggtgtgat cggataatcg
     4921 tggctctcgc ttaccacttc cacatttgta attccgattt cataaccaaa ttttgaacgg
     4981 ctgtcttcac ggatttctcc agttactaac acggatgttt cttggtgcag atttttagct
     5041 gtttggaaaa cttcttccgg cacttcgctt tttacaacta caccttggaa aaaggctgtt
     5101 ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc gcttatttgc gacccaagcg
     5161 ccgattgtta ctgtttctcc cacatggttt ttcgaatcga tgatttgaat ttgttccacg
     5221 tatgactctc cttttatatt ccttgaattt cttcaggatt aattttgact tttactctca
     5281 ataaattgct tgattctgcg taccgcttct tctaacgtag cgcgatcagt ggcataactc
     5341 atgcggacat tttctggtgc cccaaagcct tcgccagtca caagcgctac ccctgtttct
     5401 tctaaaagat cttctaccca ttgtgtgaca ttttcatact tacacatgcg tagcgtttct
     5461 tttacattag gaaaaagata gaaagctcct tgcggctttt caagagaaac acccggcagt
     5521 tctgccacaa gcggatataa cgtattcagg cgttcttcaa aggcgttccg catttcttct
     5581 actgtatctt gctcaccttt caatgcttca acagctgcat attgactgac tgctgtcgga
     5641 ttgctagttg attgggaagc aacagcgatc attccattga tgatcacagg atttcccacc
     5701 gcataaccaa tgcgccagcc tgtcatcgca tatgttttag acacgccgtt gatgatgatg
     5761 gtctgatttt tgattgcttc tgaaatcgtt gcaattggag taaactcatt gccattataa
     5821 actaaacggc cataaatatc atcagcaact atcaaaatat cattttttac tgcccattca
     5881 ccgattgcct gcagttcatt cttgctataa atcattccag tcggattcga tggagaattg
     5941 atgattacgg cttttgtttt ttctgaacgt gcttgttcta gctgttcaac agtaactttc
     6001 cagctattgg attcttcccc tttcacaaag acaggaagtc cttctgccag ttttacttgc
     6061 tcaccgtaac tgacccagta aggcacagga atgatgactt catcttgcgg gtcaaggatc
     6121 gtttggaaaa gggtatacaa agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt
     6181 tcatagacaa gtccatagtt tttcttcaga taatcaacga ttgcctgtcg aagctctggg
     6241 attcctgctg ttggtgtata atagctagct ttcccattgc ggattgcttc gattgctgct
     6301 tcttggatgt tttctggtgt cgcaaaatcc ggttctccca cagtcaaact cagcacatct
     6361 ctgccttttg cttttaatgc cttggccttt gccgcagctg ccaaagtgac agacggttct
     6421 aatcgttttg ttctttttga taactccatc ccattcattc ctttaccttt taaattccct
     6481 tgattgtctt gacttcatct ccagattcaa agccaatcag gtaataattc agtttcccat
     6541 ttgtttcttt tcctactact tcccacacag gctgatcatc atacatccct aaagcagctt
     6601 tttcgatttg gatttcagga tgggccgcag caattgtctg ttttgcttcg ttttcagtca
     6661 gtccctttgt ttgatccaat acttttactt tttcaccaga tttaggaatg ataacggcga
     6721 tttcttttcc tttatcattt gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc
     6781 aataaaaatg atcggtttcc tcaagatttg catatttctt tgcgatttct actgcttctt
     6841 tcttcgcctg caccgccgga cgactggaac gcaagtaaaa aatcgagctg atcacgatga
     6901 ccgctaataa aaatacgatc aagccaagta acacgcgatt aatcgttttt tcctttgtct
     6961 gttttttctt caagggtcgt cgctccttta tcttcattcc ttttcattat agcagatttt
     7021 aattatgaaa aaagacagct cctctaactt ccttcttcat ttttctttaa gaattcatgg
     7081 atatcctgat agatttcctc catagctagc tctttgatcg gtactttttt cggtaatgca
     7141 tggatgatcc gtgcgccata ttttgcagtg actagtcttc gatccaaaat aagcaatacg
     7201 ccacgatctg tctccgtcct tatcaagcga cctaatgctt ggcgtaactt caaagctgct
     7261 ttaggtacgg attcctggta aaaaggattg atcccctctg attctaaata agcgttccta
     7321 gctttgacca tcgggcgaag cggattatcg aagggtaaac gtgtgactac aacgatctgc
     7381 aaagcatctc caggcaaatc tacaccttcc caaaagctgt cagcgccaaa caatacgctt
     7441 ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa cccttgagct
     7501 agtatttctc gtcctttttc tagcatagac agatgcattt tctgatagac cttctgcaaa
     7561 acatcatgag aagtgaataa cacaagtatt ggccgatctt cttgttcgat cagacctgta
     7621 agtgctgtct ctaaataatc gatatatgct tgattgctga cttgatggac ggcaggtgcc
     7681 tcttttggca catagatcct tgcctgtttc tcataatcat aaggagaggc aatcacttta
     7741 agaggtgctg cttcaatgcc aagcctctta gagagatagt gtcgattagg gccgatcttc
     7801 agcgttcctc caatataaag aatacgatca tatcgaggat accaaacagt cgacggcaac
     7861 atagccgctt gaaaatcatg gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg
     7921 aaccaatgga tcaaatgatc tcgccagtct tccagccatt gatccatcag caagtattgc
     7981 ttgtctaatt gatcaaatag gctatataaa cttgctaaat cgatccgcat ctggtttgtc
     8041 cagctttctt ggataatctc aaaagtcaga cgcatttgtg actgtagctg ctggatctcc
     8101 tgataaaaaa gctgcaggtg atcaatggct gtcccagatt ccagtggcag ctgatccaac
     8161 atctcttttg tcatcatgac ttcttctgga tactgtccgt taggttgctt gccgctcact
     8221 aaactcatta atccttctgc catacaaatc tgtgcttcgc atagcgcaga aagttcttct
     8281 cgataaatcg ataaccagcg taaggcttca tgttctttcg gtaaatagct ttgtattcgg
     8341 tcaaacagct gatttggttc ttctaattgc tggatttgtt tatggaaata gttaatactt
     8401 aatcgtttgt catttacttt ttcagcgatt tccggcaaat gatgtacttc atcgatcaac
     8461 aaaaatggac tagctggaag caatggttct tttcgctgtg tttcttgtac taaaaacgca
     8521 tgattgacaa tcaaaaaatt actttgcctg attttctttt gcaagaaacg aacaaagtct
     8581 tgttcgtaaa atgactgatt tcttgctaaa aatccaatac ctctgtgcgc gatatctgca
     8641 aagaatgaat ggttcaaatt tgttaaatgt aattcatcca aatcaccagt cttcgtttgc
     8701 gtcagccaaa ctaagaccgc catttgatac aaagcatatt gtttttgttc tggcggttcc
     8761 acaagtgtgc ctttaaaacg ctgcaaatca atatagtggc gatagctttt gataattaca
     8821 ggttgtatgg gctgatctag caattgattc agcaatggaa tgtctttttc tatgatctgc
     8881 tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt agccaaataa
     8941 ctcaatggca gcagataacc gattgtttta cccattcctg tagctgcttc gatcatcaaa
     9001 tcttttgatt catcttttgt gaaatggtcg tagaccgcat tcatcagtct gttctgttct
     9061 tttcgataca tcagtgtttt accaaacatt ttttcttttg cttgtttttt tcttggatat
     9121 gtccgctcgc cataatggac ggatgtgaat agttccactt cttttttctg caaagctagc
     9181 ccatcgacga tttctaatga gggatcaagt ggctctggtt tttctttcat ttcttcaatc
     9241 acatgataaa tgaaacgact cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc
     9301 tctaacgtga tgattggcaa ttttctcatc cgttcttcga tcagcaaaag aagttgacct
     9361 gtcacttcgg catcactatc tgcctgatga ggattctcat gaataaatcc aaggctttct
     9421 gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc aagttctact
     9481 gtatcgatgc ctggtatctt caaactagga agtccgcatc gcatgagttc atggttcaaa
     9541 aaattataat caaaataaat attatgcgcg acaaatatgg tgtctgccaa aagattgtaa
     9601 atcgtttggg cgacatcttc gaaataaggc gctttttgga cgcgttgatt cgtgatccct
     9661 gttaaatgct ggatttgttt tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt
     9721 gaagtgatcc gacctgattc aatcatgacg cagccaaatt ggatgatccg atcttctttt
     9781 ggattcgtcc cagtcgtttc gatatcaacg actgcataag ttcttggttt caaaatatct
     9841 tcctcttctc ctgcctctaa tgttcttttg aaattatact acaaagtgca gaaaaatact
     9901 ctcatcgctt tttaaagaaa atagacaaaa acgcccagac acgcaaatgt ctgggcgttt
     9961 attttttagt ataggttaat taagctttag taacgtttgt tgcttgaggt ccgcgttggc
    10021 cttcttcaac atcgaaagtt actgcttggc cttcttctaa agttttgaat ccgtcgcctt
    10081 ggatagctga gaaatgaacg aatacatcgt ttccgttttc tgcagtgatg aatccaaatc
    10141 ctttttctga gttaaaccat tttactgtac ctgtttccat aataaaaatc ctccttgtgt
    10201 atcaacacat attttgcaat tacttgaatg gtgtatatct ggaagatgtt tatttgaaac
    10261 aagctaccac agattaccga acaaaaacta ctcctatagt atatcatacc gtttacaaaa
    10321 aagctagagt ttttcgactt tttttattaa aaaaagtttt ctcagcgatt acataaagtg
    10381 aaaaaccgaa ctttttttta aaataaacga catattgttc agttggtgtt tttttgctct
    10441 gatgttcttt tttaaactaa aaaaattatt ctttctgact acttatcggt tctgtttcaa
    10501 cggtcgaggc tggggaagat tctttagtag gtctgatgtt ctcatggtat ttctgatagc
    10561 ctatgacagt taccagaaga acaaggcaaa ccaatacgac caacgagatt agtttttctt
    10621 ttttgctcat atctatacct cataaataac aggatgatgt ccatcatatt tctgagtatg
    10681 agtaaaagca aaagctgcag ctgtagaatg taagaaatgc cagcaacttt cataatcaca
    10741 ggtcggacag taccaaaagt aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg
    10801 aatcgcagaa tcaagatctg gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt
    10861 aataggaaac cgctgtgttt cttcagcaaa aactggctga cttgttaaac ttagggatag
    10921 agacagcaaa caccataaca gtcttttttt cataaagacc tccttatcga taagtttttc
    10981 ccaattaaaa tcatatctca agaacgcccg ttattcacat tttgttcata aatgatgttt
    11041 tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt tttaaacggt
    11101 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaaataatt
    11161 tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct cttccttaaa
    11221 cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat atgaagagct
    11281 atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac ctctcataca
    11341 cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta
    11401 tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac ctcttttaat
    11461 aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc aaaataaaca
    11521 cttttccgcc attaatgatc acatcatctg ctaatgcatt caacacttga cgccgatcat
    11581 attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg ataaataaat
    11641 gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg atgaatttct
    11701 tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt gacagcttct
    11761 tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt
    11821 ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt tctggtagag
    11881 caaataaata aatcggccat tgttctggat tttcgaaaga atccttcaag tacgtatcaa
    11941 ctgcttggta atagttgacc caatcgatct ctacttcttc atcttttgga tttacgccat
    12001 ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc aaaccgccac
    12061 ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct ggtagagata
    12121 cttcttttac ttgtttgttg ctgacttcat acaatttcat ggagtcgcgg ttcaatccca
    12181 ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa taaggaagat
    12241 cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag gtttggtcag
    12301 cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga tctcctaaca
    12361 aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt ttctcaaaac
    12421 gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc acatcttgat
    12481 gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct tctgaagtca
    12541 attgtgacaa taattcttta tccttttttg ccataaacgt acccctccta aaaaaatcat
    12601 tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata aaacgtatct
    12661 aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt tatcttcata
    12721 atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct gataaactaa
    12781 gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt ttattaagta
    12841 ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca cagcctattc
    12901 ctgtaaaagt acgtaaaaac gaccgctaac cttccataac gtgcgttctt atcctaaagt
    12961 tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt gtagcgaaac
    13021 aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt cctattattg
    13081 gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca agctgaagat
    13141 actttcaaag taaatgcaaa agcagccttt gctgtagatg cgcaaagcgg taagattctt
    13201 tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat catcggtctt
    13261 tacatcgtcc ttgatcaagt aaaagaaggg aaactatcat gggatgataa agtttcaatt
    13321 tcagattatg cagaaaattt gagtgtcacc ccagatttat caaatgttcc tttgcataaa
    13381 gaaaacacct acaccgttaa agaattattc gactctgcaa tcatccagtc agccaacgct
    13441 tcaatggttg ccttggcaga aaaaatctct ggcagcgaag caaaattcac tgaacgaatg
    13501 aaagaacaat tgaaagattg gggtatcaaa gatgcaacga tcgttaatgc atcaggactg
    13561 aataactctt atcttggcga aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa
    13621 atgtctgctc aagatgtggc tatcgttgcc cgtcatttga tcttggattt tccagaaata
    13681 ctggatgtat ctagcactac gacacaaatg tttggcgaaa acacgcaatc accagtagaa
    13741 atggtcaact ggaactggat gctcccagga ttcgtgaatt ataaagaagg cgtggacgga
    13801 ttaaaaactg gtacgactga acttgcagga gcctgtttcg tcggtacgat cactaaaaac
    13861 gatcaacgaa tcatcactgt cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc
    13921 cgtttcaatg aaacagctcg cttaatggat tattgctacg acaattggtc agaaaaagag
    13981 cttggaaaag ccaatgcaag tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa
    14041 gaatcaacag tgcccgttgt gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg
    14101 gatactggaa aattaacaat cacaccaact cttgacaaaa aacaagtcac tgacaatgag
    14161 atccaagcac ctatggataa aggaacaaaa atcggtaccg caacgatcac cttggcagaa
    14221 gataaattag gttatcttga agacaacaac gcgccatcca cagaaatcat cacggataaa
    14281 accgtagaaa aagcaaatat tttcgttatt ggctggcgac gcgtcgcaga atttttcggt
    14341 aacttgtttt aattgaacaa aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg
    14401 ctttttctag tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa
    14461 ggagttgaat ttatgaatct aaccagagat ctgatttttt tggttctcta tcttattggg
    14521 gtattctcca tcgtttattg gcgaaaacag aaaaaggatc cttccttgtt cttttacagc
    14581 agtacatgga cgtttgcggt aatcttgact tattttgtcg tgttggaaat tttttattcg
    14641 accttttatt ttttgattcc cttagtgttt ttcagtatct ttgccttttc ctatttttca
    14701 gaaaagcgaa ggctgttgaa tggtctttta ttcaatattt tcctcattag ttttggtatc
    14761 tacttgtttg tcttgctata cgaaacacaa aatatttttc ttggcggact gattgccttg
    14821 atcacgatcc cgttactgct tgtctttcta ttcggcattt atggattgat cgtttttctt
    14881 ttttggaacg gtgtcacagt tttacgtaga gagtcgcatt cattagctaa tcttttaacg
    14941 ttgatactag ccatttttct tacattgttt cttgtttttg actttttctt gttaaaatat
    15001 ttaccacaat ggataaatgc tttgtttttc tgcatcccat tgatcttgat ctatctattt
    15061 atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg ccctcgatac
    15121 aaccaagact tcatcgttgt gttaggcgcc gggttgatca atggagaaac agtttctcct
    15181 cttcttgcca agcgaatcaa taaggcgatt gctttttatt gggcacaaag ccgtgcgaca
    15241 ttaaatccgc ctatcctatt aatgtcgggt ggacagggag cagacgaaaa agtaccagaa
    15301 gcaatggcga tgaaacaata tgccatggaa caaggaattc ctgaaagaga tattcttgtc
    15361 gaaaccaatt cgacaaccac tttggagaat atgcgctatt caaaggaaat catggatcag
    15421 caaatgaaag gtccctatcg cgcgatcttc agttccaaca attaccacat tttcagagct
    15481 ggtctctatg cacgacaagc gaaactgaag gcaaatggca ttggggcaaa aacagctttc
    15541 tattacttgc ctaacgcgtt tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa
    15601 agacatatga tcatttgcgg attgcttttc gcaggttccg cattcttatc actgatcact
    15661 ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttt
    15721 attctcttga cgattggcaa gaatagtatt ccttttttga cggctacttg ctgtgcagaa
    15781 taaataccgc tgtttcctga acacataaag ctgatcaagc agatcataaa gaagtaagca
    15841 gcagcttgtg aaccaaataa ttcgattccc atcacaaaac aggcaatcgg tgtatttgtc
    15901 gcaccagaaa ataccccgat aaaacctaat ccagcaagaa aaggaacaga aacatggagc
    15961 aaaggcgcta gtactgcgcc aagcgttgca ccaatctcaa atagtggtgt cacttctccc
    16021 ccttggtacc ccgcacctaa agacaagacc gtgaagaata atttcccaat aaaatcataa
    16081 gcatgtgcct gtccattaaa tgattcctgc agtaacggaa gactcaaccc aaggtaacgt
    16141 tgtgtttgca aaatcaacgc tgcggctaca actactgctc cacctatcgt atttcgccaa
    16201 acaggatgag gaatccattg cgcataccgc gcttttaaaa agacgatcga acggctgaat
    16261 acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata
    16321 ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc
    16381 atgaaatttg caaaaagcgc agcaaagaag ctaggaaata ttgcttctgt tcggacttta
    16441 ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa
    16501 ctaaaacccg cactgattcc actgataacc aaaatctccc gttctgcttt atccaatcgg
    16561 aataaatgcg ctatattatc agctaatgct ccccccattt gaactgctgt tccttcacga
    16621 ccaacagatc ccccaaataa atgggtagtg atggtcccaa acaaggtcaa tggaatgagc
    16681 cgcaaaggga tcttctcttc tcctccattc ccttgatcga tcactaagtt attcccacga
    16741 ctagaaagac cgccatgata cgcataaaga taagcaaaaa gcgcgccgct gactggcaat
    16801 agataaagta gccaaggatg tgctagtcgg atatctgtca cgaacgtcag gctatttaag
    16861 aaaaaagcag acaagctccc catgaatact ccgagaatcg ctgtgaccac cagccacttc
    16921 aacatgtaca tcaaaatacg cactggactc ggtaattgtt tttccataaa aaattcctcc
    16981 tcaaataaaa aaacgcctac taaagaaagg ggctggcccc agactttagt aggcgtcatt
    17041 agctatctta cgttagcatt taaaggcgaa caccatcacc tattcacttc cattaaaaga
    17101 tagcatattt ttctgtaaaa aacaataaaa ttatcgttat ttctttacac gttcagcaaa
    17161 ttcatagaaa ggagccaatt tcgttaactg ttgattacat tgatcactat catgacaaat
    17221 ataattcccc tttttcgtat acgtgccatc agaaccggct ttcgttgtag aaagaaagag
    17281 cgagacattc gatgttttct gacagatcgc acagattccc ttattaatcg ttggtgataa
    17341 agtaccgctg atccctttta attttccatc ttcgtaataa accatgaatt ttttttgcgt
    17401 ccctggatca ttccaaccga tgtacgtatg tgcttgcaag tcgatttcat tccaagcggg
    17461 ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc tcggctgcat
    17521 aaacggctgg acgatttgct tcagctcctc caatgctcgt tctgcttgcg tttttgtcag
    17581 ttttacatct aaaactgtcg ttaaaaacgt atcgatttct gatggttcgt aacctaatac
    17641 atcgcgaatc ttttcagctg ctagagcctg aaccgtttgt accgtttttg agtcattgac
    17701 agatgagtac gcatttaata actgctctac ttctcttttt ataaaaaaat attgatatgg
    17761 tttcattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc atactttgta
    17821 gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcccggactc ttttaaataa
    17881 gatacgtttt gcatcttatc atcaccccct ttaatgttca ttcttctatg tacatgattc
    17941 attcgataaa taaaagtaaa ccgtatcttg agcagatagt caactgcaaa cagaaaaagg
    18001 actgtgacaa aagtcgtgtc aaagtccctt tctacaaaca aacggtgttc cagaagtagc
    18061 ttcttcaaaa aataagccgg aatttcacaa aaatttgaga agcaattttc gtaaattcct
    18121 tcttatttct tgaagctgac ccacttctgt cacaacctca atatggtcta ttattggttt
    18181 tacctgttaa atcgaggatt cttttccgaa gtaacgttgc catctcttac gcatacttgc
    18241 tgacataggc tgagcggctt ctttgactgc gcagtatttg tctacgaatg ttacgatata
    18301 tccttctttg tacttaggcg gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc
    18361 tcgctcaaga tccgacaaat cggtgatctt ttctgcattc tttacagcga ttcttggatg
    18421 gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc caatcatagt agaacagatc
    18481 atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag
    18541 ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt
    18601 gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttctacat aagacatata
    18661 ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc
    18721 attttcttct gaaattgatc taagtatacc accagtgttc ggattattct agtcgccttg
    18781 gataaaatag ccaaagctta atagttcctt aatcataaag aaaaatgata aagaacaatc
    18841 cctgccgcaa ccccaacatt caacgattcc gcatttcctt tcatcgggat atatacgttc
    18901 tgatcagtca aagacaagat ttcctgactt accccctgac cttcgttccc catgattatt
    18961 gcgaagttat ctgatttttc tactttgtct aaggcgtgtg cttcttcatt taattctgtg
    19021 ccatacactg taaacccttt gtctttcatt aaaggaatca cttctgtcaa atctttttgc
    19081 aacacaggca aatgataatt gcttccctgc atgctgcgca gcacctttgt actataaata
    19141 tctgccgttc ctttgcctaa taaaacacca gccatatcaa atgcatctgc cgtacggatc
    19201 atcgtcccta catttccagg atcttgtaca ttatctaata acagccatcc tcctgaaaaa
    19261 tctggcgtgc cttcagccgt taacgccatc actgctaaga ttccttgtgg tgtaggtaga
    19321 tccgatacag catccatgac ctcatccgaa actagaaaag tagaaatctc tttgtttgcg
    19381 gccagccagt ctccccattc tttcatgcct cgctgtgtca caaaaagttc ttttatttct
    19441 gcatcagacg acgcagcttc ttcaatcaga tggaaccctt ccaaaagata ttcttccttt
    19501 tcttcccgat atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct
    19561 gattggatct ctttcacatt cctcacctcc ccatgattat atcatggaat aggtgcttga
    19621 gcttgtttca tcctaaaaaa atcggtatca ttaatttata aaataggaaa gaaaggtgac
    19681 gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta
    19741 catgaccaag atggtcgctg atcaactagg cgtgactgga acggtaaaaa acgaagatga
    19801 cggctcagtc acgatcgaag caatcggcaa tgacgacatc atccagaaat ttattgaaga
    19861 agtcaagaaa tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagaccc
    19921 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aagctggtct
    19981 gcatcaagca ttaaggacag gttgtgaaaa atagctctcc atatttttca acaggctgga
    20041 cttaatgccg tagatgcaag cttttgaaca ccgtcaataa ggttgtgaga aagtcgctta
    20101 gttttgagta gtaaggagaa ttttgtgaaa atggctttcc acatttttgc ataaaatttg
    20161 acttaatacc gagaaactga cttttgaaca ccgtcgataa ggttgttttt gagacgcttt
    20221 gcctcaagta ttaaagatag gtagtaaaaa agctgatcta taacttgcaa taaatttgct
    20281 taacccatat tttccagaca gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca
    20341 cttggaatca ctcttttctt tccaagtatt tctttcttga ttttttgtct ttttttcaaa
    20401 atttttcgct acgtaaaaga acgaatattt atatgaaatt tctatgaagt tgattgaatt
    20461 ccatgcgctt ttttagtata gttaagtttg tgtaaaaatt atagaagagg aagtattcta
    20521 tgaataaatg gaaaaactgg ttattgggat ccggacttgt cagtatcctg ctctttttat
    20581 ctggctgtgt gcggatggat tcaaacggaa accctgacac ttcgggaatc gtttatcgag
    20641 tactcgtcca tcctatgggc caagcgatta catatctagt tgagaatttc aattggtctt
    20701 atggctgggc agttattgca atgactgtga tcgtccgtat catcatcttg ccactaggta
    20761 ttagtcaatc gaaaaaaaca atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag
    20821 ttgaagcagc ccaacaaaaa ttgaaagctg caacaacacg tgaagaacaa atggcagcac
    20881 aagcagaaat gcagcaagta taccgcgaaa acggattgag tatgaccggc ggaatcggct
    20941 gtctgcctct attgatccaa atgccgatct tctctgcctt gtactttacg gcacgttata
    21001 cagaaggtat tcgggaatca tctttctacg gcatcgattt aggctcacca agtatggtac
    21061 ttgttgtcat tgcaggggtc gcctatctgc ttcaaggata catctcaacg atcggtattc
    21121 ctgaagaaca gaaaaagacg atgcgtacga tgctaatcgt ctctcctgct atgatcgtct
    21181 tcatgtcgat cagtgcgcca gccggtgtta cactttactg ggtagtcggt ggtgtgttca
    21241 gctgtctgca aacattcatc acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg
    21301 cagaagagtt gaaacaaaac ccgccaaaac aagtcgtcac tccaagaaaa gacgtgacag
    21361 aatcagcaaa gccagctaac aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca
    21421 gtacaaacaa caaaggccgc aacgcaggaa agcagcagaa gcggaaataa aaacatagga
    21481 caaaaaagat gagagctaag ccctcatctt ttttgttact cttcctcttc tgctattcca
    21541 ttaattaatt cgttgacaga gacaatgctt tcacatcctg cacgaacaaa ctcgggctct
    21601 gaaccaatca tttgtgagtt gataaattca atgaccatcg gaagattcat cccagcataa
    21661 agagtaatct gttgtccttc cattattagt ttagaaatga tatttgcagg tgtaccacct
    21721 aaaagatcgc atagtaccac aaaatcattt aacgaatctg tgacagtgag aaacttttgt
    21781 ttgtatgttt cagtgccatc ttctggtaat aaaggtaacg tgtagatatc ctcttgaggc
    21841 cccatgatca tttctgtact ttttttcaat tcctcacaaa attttccgtg actaatcaat
    21901 accaattgtc tactcatttt tcgctctccc ttatttcgaa atcacaccca atgcagataa
    21961 agcaacacac acaattaata cgataaaaat cgctcgagta ggagtcatgt ttttctttcc
    22021 taataaccaa taaacaccac cgacgatccc agcaggtaaa attttaggta agatcatatc
    22081 gatatagttt tgcatattca atgtcacatc gccaatcgtt ggtgcccatg aaaatttaac
    22141 attgatcatt gtcgcaacta atgcccctac catgaatacc cctaataaag tggctgcgtc
    22201 cgttaatgca gttaagcgag attgcatagt agtaactaag gaaattcctt ctttgtatgc
    22261 aaattcaagt tgcttccaac gaaaaaccat gacaacgatc tgagcaaaaa tccatataaa
    22321 cacacctatc ggatttccat cagttgccat attcgccgcc aaagccccaa aaatcgttgg
    22381 aatcaaagca gcaaatacgg aatctccaat cgctgcaaag gaacccatca atccagcttt
    22441 cagccccgat acggtttcct ttgaagcaat tccctctttt tcttccattg caagatcaat
    22501 cccagtaatg attgtattaa aaaaattaga agtattaaaa aattgcgtat gtgttttcat
    22561 catctctttt aattctggtg tattgtctcc atatattttt cttagttgtg gcaagatagt
    22621 atataaatag ccagaaccct gcattctctc atagttccaa cctaattgaa aagtgaacaa
    22681 acttcttcga ttgatttgct taaaatcttc ttttgttaat ttgtaattag atttcgtcat
    22741 cttcgatttc cccctcaaca gaatgtgatg gctcactagc taatggttga tggtctttgt
    22801 tttgatcatt cttataactg atcattgcta gtgctagacc aatcaatgct acagcaagca
    22861 tcggcagcga attgaaagtt gccggaagat ctttaacaac gcttgccaca cttgttccta
    22921 ataattgaac attagagaat aacgttgcta ataatgccgt gatcgtaaat cctaaaataa
    22981 gataagatag atgttttttt actggtaaat agcgcagaag aatggcaaac ccaacagctg
    23041 gtaaaacagc tcctgctact gaaagtccat cgcctagcca ttttagattc ccgtttaaaa
    23101 cagaaacaac attttcaact aatccaccac caaatgctaa agctaaaaat acaggaatca
    23161 tacgagagag cgaccaaggt aaagctccca ttaagaaatt gcgttcgatt gcttgataat
    23221 tcatgtcttc tacgtgacgg tcaatacgat gggcaaagta tgtgttagcg aatctagcta
    23281 aaatatccaa ttggatcatt aagcttgcta caggcactgc aatagcagca atcgcttgtt
    23341 ctggattcat tccaagagaa atcgagaagg cagttgctaa aatcgtccca gagttggcat
    23401 ctatttttga tgcgcctcca aacgtcccca ctcctaaaac tgtcaactgc atactgccac
    23461 caataatcaa acctgcgttt aaatcaccca ttactaaacc tgcatataac cctgcaaaca
    23521 ctggcgcact cattgaagaa tagatctgta attcatctaa aatttgataa cctgcatata
    23581 atgttaataa taagatctgc caccattgaa tatccatgga tcattcctcc tatttgatta
    23641 aaagattcat aaagtcttct gctttatcgt ttggtaccat ttgagcaatt aaatgtatac
    23701 caagctgatc taatttttta aatacttcga tatctgcatc taccacattg attgattttg
    23761 taatggaccg tgtttcattc gtttgtgaca tattccccac atttacttct ttgataggaa
    23821 ctcctttttc tactaaagat aagattcgat ctggcttttt tgctacgatc aatactcgtt
    23881 gagagtcata tttgccagct aaaatatttg ttgctgcttt ttcgattggc aaaatgctta
    23941 attttacccc tgctggagtc gctaacttca atccactttt ttcaattgtg tttgtggcta
    24001 cctcatcatc cacgaccatg attcgactaa tattcagttt cgttgtccaa agatttgcta
    24061 cttatccatg tatcaaacgt ccatcgattc ttacaccaat aattgccatc atgcattctc
    24121 cttatctatt ttttctattt attgttcaat taaaactgtg acatcatcaa atatcccttc
    24181 tgtttcaaag ataacgtttt cattttttct ttcttatatt tcatttttaa gctaagaaca
    24241 aattccttcc caactaaaga aaacgctttc ttttatattt aagttcattt gattttctta
    24301 acttgaatgt gtttcatcaa catcgtaact ttcatagggt cgatccagcc agttttctct
    24361 tgttgcgcgt ttgccatacc acaagccata gaaaatgcga gaatctcttc gattgattgc
    24421 ctttttgata acgcatgggc cgatcctgca agggtcgcgt cacctgaacc aacaggatta
    24481 acaactgata ttttaggaat aatgacttcg taaaaacact gagcaacttt tgccacagca
    24541 cctctctctc ctaaagaaac aacaacccat ggcactcctt caaatattgg atgagctaat
    24601 aaggactgaa ttttttctaa ttgaatcgaa gaacagatac gttggccaat cagttctcct
    24661 aattcttcca gattgggctt gataagtagt ggcttctcct taccttccaa tacttcaagc
    24721 aaaaatttac cagaagtatc tagtaatatg tctttccctt ttttatcagc taaagcaacc
    24781 aattgcttat agtaatcaag agggcatcct tggggtaaac tacctgagat cgtcataata
    24841 tctgatattt cgaggagctg ctcgaaatga tcggtaaatg tctgttgttc tttctttgtt
    24901 atgaaggggc ctcgttccaa aagctctgtt tgtctcgaac tatgcaggat agcaatagaa
    24961 tcccttgtct ctcctttaat tggaacaaac tgatgcatga tccctaactg atctagctgg
    25021 ttctttagat attcaccgtg ctggccacct attaaaccag tagctatcac ctcttctcct
    25081 aataaattta gaaccctcgc cacattcact ccttttcctc ccgctgtttt attgactgtt
    25141 tcaactcgat tgactttgtc gaggtttaat tcttcaattg tatacgcgat atcgatggaa
    25201 ggattcaagg taactgtcgt aatcatttca tcttcacctt tttgcttttt gttttattga
    25261 accaaggggt agcggtttca tttattactt tatttaaagt ctcgatgttt tctctcccat
    25321 ttgttcttaa ccaatgaata gctccgtctt ttccattttc gacgtatgct ttgaccccat
    25381 ctttccatgt cgcacgtcca cacaatacac cattgaaggt ggaacccgat tcatgagcaa
    25441 aaatcagtgt ttgttgaaat aattctgttg aaacaccccc actcaaaaaa atgaatggta
    25501 atggagtcgc ttcactttgc tccttaaaga aggcagcagc ctcttctttg ctgtgaataa
    25561 attcacctgt ggaatagcca ttcacataat tcatattgac tggtacttct actttcaaca
    25621 catcaacttt atattttgga tcagaaaatt ctttcatcat ctcaataact ttgtggggct
    25681 tgactttcgc atactctgga gaagaactat cgcttatttg tgcgtcataa gaaactaact
    25741 ccaaatagaa gggacgattt tctccttcgc actcactacc taaacgttcg atgaacacat
    25801 gcttcaaatg attgatttcg ggtgcttcat caacatcgta gtaaagtaag aattttactg
    25861 catccgcttg ctgttctttc aatctgagag aagaccaatc cgctaataaa tcaggtagac
    25921 gaccaggcgt agttgcgtca tagccggttt tctcataagc taacaataac cccgtctctt
    25981 ttgctctgac ttttgctgct ggcaatccat attccggatc caataaaata gcagaagcat
    26041 agggtgtcag ctcagctgat actagttctt tgaattcact gatttggtct tcacttggct
    26101 cttttcccaa cgatttgatc atttttttta gagcaccacg ctgatcgatc gctaatgcac
    26161 taataatccc atcttttgtt gatagtctct ccattgccct atttttttct tcattcatct
    26221 gtcatttctc cttcggtttc atacttatga ataatgacgc ccttgactac tcgattgacc
    26281 gtgcctgttg gagatggtgt atctggttta ttagctactt tgattgaagt atagagtgca
    26341 atgatttgtg cagtcataat catcgctaat gcaagatagg catcgggcaa tttactcacc
    26401 gagtcttcca atagcacagt catacctgag aaatcaatag tacccggttg cgcaatcgca
    26461 atgacattgg cagtgacccg atcttctttt atttcctcta aaatatctaa gtcataattt
    26521 cgcgtatatg gttgattgct cacaaaacct acaacgagtg tctcgctatt taaaaaagac
    26581 tttggtccat gacgaaaccc catcgaagaa tcaaaaattg tagcaacttt acctgctgtt
    26641 aattctaaaa tttttaattg tgcttctctt gtcgcacctg ccaaacttcc tgaacccaga
    26701 taagcgatcc gttcaaaaaa atcaggtaat aacttttgaa tttcttgatc tcttgaaaga
    26761 agatcttgac cgattttaac taattgttca agatatcttt cttttttctc cattgctgtt
    26821 tgatcaaaga tccataaagc actcaataac atacacgtaa aacttcctgt catagcaaaa
    26881 cctgcatcgt ttgctctctc tggcatcaat aacagataat tctgtttatc atcctgtgcg
    26941 ttttttgcca acatcccctc tttagcacaa gtaatcgtca aatgaaacaa tgtttcaact
    27001 tgttcatttg caatttgcac agccgctaca ctttctggac tgttgccact gcgagcaaat
    27061 gaaactaata aagtcggttc atttggaaac aaaaattctt ttggtgaagc tacaatatct
    27121 gtcgtgccaa aactgtggaa gataaaaaac tgattgtctc catgttcatt tagatagggt
    27181 aagagcgtat ccccaatata ttgagacgtt cctgcacccg taaaaataac acgtattctt
    27241 cttccgtttg cttctttttt tatttgatca aaaaatagag ctaaattttc ttggctttgc
    27301 atcaatctat cgattgtttc tttccagaga tctggttgtt gttttatttc ttgtgtcgtg
    27361 attgctgcac ctatttgttt taatttgttc ttcgatagct caaacatgtt gttctccctc
    27421 tcaatcattt tcggatatgc cgaattttat aatgaaactg gtccgctctt gctacactat
    27481 ctgtatattc gataatctca tttttcacgt tataggttat cctcgtcaga tgtaagacgg
    27541 gagcgccatc agcgattcgt agcacaacag catctttttt ttgcacgata cttgcataaa
    27601 actcttcatc agctaattga acttgttgtt cataatcctg tgaaaacaca tcatacaacg
    27661 gtttactttc aagtaacggc aaatcaagtc ccataaatag tttcgccggt agataagaac
    27721 gctcaaccat caatggctct tcatctgcca aacgcaatct tttaagttta aatacagttt
    27781 cacctaaatg gatgttcaaa ttcttagcaa ttctcttatt agcttccatt tgtctaaatt
    27841 ctaaaatgac cgtcttaggg acgcgcccta tttcgcgcat ttcttctgta aagctatatg
    27901 ctccagctaa gttcgatgac ttatcagaca aatcagagac ataggtacct tttccatgtc
    27961 ttcggtaaat ataaccttga ttttccaatt cctgtaaagc cagacgtacg gtggtacgac
    28021 tcaccccata gtgttgacaa agttctcttt cagataacag cttatcatgc ggagtcatct
    28081 gtgtttcgat acgatccttc aataaatcaa ctagttgata atacaatgat tttcctttat
    28141 ttgagctctc cataaaaatc acctcttctt tactggttat aaccacaaca caagaatagt
    28201 gaattattaa tcatttgtca aaggtttttt agaccaaaaa agctattata acagctcttt
    28261 aaagctttat atcttgaata gacaaagata cctcaaaaat accagttacc aataaaagaa
    28321 cagaaaaaaa gacgaaccac ataagcgatc cgtcaaaatg gtctaactta tcgagcagcg
    28381 tgatccacat taatccctat gatattccta caactatcgt tttattactt ccaatatcat
    28441 ttacaacaac taaaaatagc ttattaatga caaatgttat tattagtttt agataacttt
    28501 gggagcgtca ataattttgt gtaaatggaa aagcccattc tgttgatcaa gttaaaacat
    28561 tgattctaat gtatcatgta tttctttaaa acctttatga actcggcata gaaacttttg
    28621 attatagttg ttgaattgag atactagaaa tctctccaga gattcttcat taggaaattg
    28681 ctcttttctt ttggtatatt tctttaattg tttgttaaaa ccttcaatca aattagttga
    28741 ataaatagtt cttctgattg acggagggaa attataaaag gttaatatag caggattcat
    28801 gagcaattta accactcgtg gatactgctt tttccattta tctatcataa accttatttg
    28861 attatttgct tcttcttttg aacttgcttg ataaactaat ttgaaatcgc tacagatttc
    28921 ttgtcgatca ctaacacgaa ccttatgagc gatatttcta gatatatgga cacaacattg
    28981 ttgaaattga gcatttggat aaacactatg aatactatcg ttaatgccac ttaaaccatc
    29041 agttacgact aataaaacct cttttaaacc acggtctttt aggtcttgaa gaatctcttt
    29101 ccaaacataa gcagattcag ttggagcaag actaaaccca agaacctctt tggttccatc
    29161 taatcgaatg ccaattacaa tataaactgc ttcttttgaa acagtttgtc tttttaaagg
    29221 aatgtgagta gcatccataa aaatgacaga gtatctggat tctaaggacc tttctttaaa
    29281 ggcaataata tcttcagaaa ctattttagt catatttgaa atggtttgtg gcgtatagtg
    29341 atgcccgtac attttttcaa tcaattcaga gatttcagac atagtaattc ctttttgaaa
    29401 tagctgaata atagtggttt ctaaagagtc attggttctt ttataagcag gtaaagtttg
    29461 ttgagaaaac ttaccatttc tatctctagg aattaccaaa tttaattctc catactctgt
    29521 tctgaatgaa cgtgaataat tcccattacg ggaattacct gaattgaacc cagcacgatc
    29581 atatttttca taatcaagaa aagctgttaa ttcagcctgc aataatgaat taacagctaa
    29641 ttctaaatga catcgaaata attcatctaa atcaccttta ttgattagtg tttccataat
    29701 ttctgtagta aaattgggca tgagaaagtc ctcctataaa atttcggttg tggtaacttt
    29761 aattttacag aatggacttt ctttttttct accctaaata tctatttaca caaaatattt
    29821 tacgctatca ttttgtctgt gtagatattt cgttatatca ctaaagataa taagatttaa
    29881 tgacctcatt aatattgttt gctaggtcca aactcttatt aaactttctt tgctataaaa
    29941 cccaaacata gtaataaaag aataacttat tacctttaaa ttatactacc aacaaatggt
    30001 agtatattat taatttatat aatatgaaac ctaaaatata gtagtatgtt aatttatact
    30061 aaattaaata aagtactacc tttaataggt agtactttat tatcaagcca ttattctttc
    30121 ttctactagt tttttttgtc acataatttt gcaatcaata aatgttgcgc aataagtata
    30181 agaattccat tttctaaatt tgaaaggtga tccataattt catgttcaag tagtttatta
    30241 attgtccact tgtcgcttca tctaaaaatt ttataggttt taggaatgct attcgcttat
    30301 tttaggaaga ttttaactgt ttgctataga tatatatcat actagtatcg taataaaagt
    30361 actctaaaag aagaattttc aaatacgcaa aattaagtac gaaagagttg aaacaaattt
    30421 ggagaaaaat gagcatagaa tgtctgatga gttaaataga cctaaaaatt atctaaaagt
    30481 ggagcaaaat taggaattac aaacaaaaca ctaaattagt ttatataatt ttccgtaata
    30541 tttaatatat atgaaaagga aaacgtagaa aattacgtct acgttttcct atttagttct
    30601 atattttcat taaattttat atttgctctt ttcacttttc ggaataaatt cactgtgact
    30661 cttgatacct tcttttatat attgctaatg tattttctcc attgctatga aaatagaatc
    30721 aacatattta taatatctat ctaatctatt ttcatttcac cgcagcatct tattaaataa
    30781 cgtgtaaata agaatataaa agttattgaa ctaattaata aagatagaat catcgtagtt
    30841 ggatacgtag atgaaacctc aactatagtt cttttccccg tacctttaat agacactaaa
    30901 ccattgtcat tttctatagt gctctttttc ccatttactt tcacaactgt tccccaataa
    30961 ttgaatacag gtaatgtgat attttgctca tcggcatctt ctactgtata gactattttg
    31021 gaattatatg ctttatgaga tgcattctcc caccgatcac caacttttac ttcatgtttc
    31081 tttaaatgat ctttatattt caatgcatct aatggtgcat agtcatatag gcctccagtt
    31141 cttgaggatg tataacgtgt aacatctttt ttggtcataa ctaaacgacc tttatacgac
    31201 tgaattttgg ctctataatt tatgggactg atgaatcaga attgattctt cagccccatt
    31261 tttattttag gtattaaaaa acatatcgtt ctctagtatg attaaatcac gacaaaataa
    31321 acaactgaga ggacgatatg ctatatgaat gattctatca aaaaaatgct gcgattaata
    31381 gagaaagatt tgatgattac agaggtctct tacgagacct ttcagaagaa aaagacgttg
    31441 atcgtcgatg ctgttttctc acctgctccc catacctgta gaaattgtgg ttctactgtg
    31501 gtagatggaa acgggaaagt aattgtagtg aaaaatggga agaaggaaac gattgtccgc
    31561 tttgaacaat acaatcacat gcctttggtt atgcgcttaa aaaagcagcg ctatacctgt
    31621 aaaaact
//
LOCUS       DACCWV010000017        30841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCWV010000017 DACCWV010000000
VERSION     DACCWV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.017"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(232..2307)
                     /locus_tag="IVW84_000712"
     CDS             complement(232..2307)
                     /locus_tag="IVW84_000712"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7916930.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2309..3226)
                     /gene="glyQ"
                     /locus_tag="IVW84_000713"
     CDS             complement(2309..3226)
                     /gene="glyQ"
                     /locus_tag="IVW84_000713"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7916931.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3610..4422)
                     /gene="recO"
                     /locus_tag="IVW84_000714"
     CDS             complement(3610..4422)
                     /gene="recO"
                     /locus_tag="IVW84_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7916932.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4565..5464)
                     /locus_tag="IVW84_000715"
     CDS             complement(4565..5464)
                     /locus_tag="IVW84_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7916933.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5479..5880)
                     /locus_tag="IVW84_000716"
     CDS             complement(5479..5880)
                     /locus_tag="IVW84_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7916934.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5858..6334)
                     /gene="ybeY"
                     /locus_tag="IVW84_000717"
     CDS             complement(5858..6334)
                     /gene="ybeY"
                     /locus_tag="IVW84_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7916935.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6351..8543)
                     /locus_tag="IVW84_000718"
     CDS             complement(6351..8543)
                     /locus_tag="IVW84_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7916936.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8567..9538)
                     /locus_tag="IVW84_000719"
     CDS             complement(8567..9538)
                     /locus_tag="IVW84_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7916937.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10244..11623
                     /gene="brnQ"
                     /locus_tag="IVW84_000720"
     CDS             10244..11623
                     /gene="brnQ"
                     /locus_tag="IVW84_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7916938.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11779..12225)
                     /locus_tag="IVW84_000721"
     CDS             complement(11779..12225)
                     /locus_tag="IVW84_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7916939.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12252..12428)
                     /locus_tag="IVW84_000722"
     CDS             complement(12252..12428)
                     /locus_tag="IVW84_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7916940.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12590..13018)
                     /locus_tag="IVW84_000723"
     CDS             complement(12590..13018)
                     /locus_tag="IVW84_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7916941.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13110..13970
                     /locus_tag="IVW84_000724"
     CDS             13110..13970
                     /locus_tag="IVW84_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7916942.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14054..14347)
                     /locus_tag="IVW84_000725"
     CDS             complement(14054..14347)
                     /locus_tag="IVW84_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7916943.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14420..16147)
                     /locus_tag="IVW84_000726"
     CDS             complement(14420..16147)
                     /locus_tag="IVW84_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7916944.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16360..17286)
                     /gene="trxB"
                     /locus_tag="IVW84_000727"
     CDS             complement(16360..17286)
                     /gene="trxB"
                     /locus_tag="IVW84_000727"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7916945.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17431..18864)
                     /gene="ascB"
                     /locus_tag="IVW84_000728"
     CDS             complement(17431..18864)
                     /gene="ascB"
                     /locus_tag="IVW84_000728"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7916946.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18864..20267)
                     /locus_tag="IVW84_000729"
     CDS             complement(18864..20267)
                     /locus_tag="IVW84_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7916947.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20497..21399
                     /locus_tag="IVW84_000730"
     CDS             20497..21399
                     /locus_tag="IVW84_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7916948.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21440..23326)
                     /locus_tag="IVW84_000731"
     CDS             complement(21440..23326)
                     /locus_tag="IVW84_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7916949.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23423..24274)
                     /locus_tag="IVW84_000732"
     CDS             complement(23423..24274)
                     /locus_tag="IVW84_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7916950.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24732..26516)
                     /locus_tag="IVW84_000733"
     CDS             complement(24732..26516)
                     /locus_tag="IVW84_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7916951.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26516..28267)
                     /locus_tag="IVW84_000734"
     CDS             complement(26516..28267)
                     /locus_tag="IVW84_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7916952.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28409..28633)
                     /locus_tag="IVW84_000735"
     CDS             complement(28409..28633)
                     /locus_tag="IVW84_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7916953.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28884..30470
                     /locus_tag="IVW84_000736"
     CDS             28884..30470
                     /locus_tag="IVW84_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7916954.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8612 a         6631 c         5503 g        10095 t
ORIGIN      
        1 tgccatttaa aaagaaaagc gacagtttac caataaattt caactgaatt atctaaaagt
       61 agatcagtta gtttagataa ttcagttgaa attgaccgac tcaaattact tgtataatac
      121 ctaggaaaaa attaactagc accacgggta tagttattta tatttataac aaataaacca
      181 gagaaatagc ctattttgtg cctattctct ggtttattta catattagaa attattttgt
      241 gatgatttga tctaaacttg ctagcgacaa agccatggca gcaatttgac ttaattgcgt
      301 taatcggttc ttacgtactg ctggttcttc tgccatgacc atcgtctgat caaaataatt
      361 ttcgatcagt gggcgaaggc ttacaagctg atcataattt tcgttgatcg tttggtcagc
      421 aaagcttttt ttcattgcct ctactgcttt gtgcagttct ttctccgctt cattttcgaa
      481 cagttcaggg tctacttctg attgtttttc aagctccgct tttttcgcta agttcacgac
      541 acgtgtcaaa gcttccatcg atggtttgaa gtcttcatct gcaagatgct gcttgaacaa
      601 ttgtgctgct gcaaatactt ttgtcagatc tttttgttct gcattcaaaa cagcttcgat
      661 gacatcatga cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc
      721 gatcacctgt tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat
      781 aacatcacta atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat
      841 gatccgaacg acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg
      901 aatcatccca actgcaaaga aagaaaaaat gctatctaat ttatctgcaa tcgctaaaac
      961 agaacctact gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg
     1021 aatcgctgtt gctacagcag gtttctcgcc tttcagcaga gcatattttt cgcccatgat
     1081 cccttgtagt tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc
     1141 agcagctcgt tgcagatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat
     1201 acgggcaatg acttgtaccc gctgcatttt ctcatagata ctaccgattt tttcatggaa
     1261 agtaacagtt tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata
     1321 aaagaattcc gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatt
     1381 ttccaatttt acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg
     1441 atcgttccgt acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc
     1501 aggaacagaa agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa
     1561 attgttgact tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttcggc
     1621 taattcattt gcttgtgtaa cgatcatttc ttgtcttttt tgcggtgaag caataacgaa
     1681 ttgttcattc aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa
     1741 gaaacgatgt cctcgagagg ctctgcctgt tttgacatct aagatctcaa acgggattac
     1801 ttcgtcatct aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc
     1861 tgcccaatgc atcgttactg ggaaagtgag gcttttaatg acatctgcta aacctgtcaa
     1921 tacttctttc gcttctttgc cgtgagtgta tttcgttaca tagacatatt ccacgccttt
     1981 taattctcta aaagtgatag catctgtcgt cagaccttgt ccgcggacaa atccttcagc
     2041 tgctttcgtc cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc
     2101 ttcttcactg tctgcttgac gttctggaat gtttgttact ttgattgcta agcggcgcgg
     2161 tgtagaaaac gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt
     2221 tttttcttct aattgtttca tgcttggtgt gacaacatga gcaggcattt cttccagccc
     2281 gatttcaagt aataagtctt ttgccatctt attcagcctc cttcatgttt tctggatttg
     2341 ctgtgttttt attcaataaa gggaatccta atttttcgcg ttctgccaca aagattttgg
     2401 ctacagcacg agccatgttg cggatacggg aaagataacc tgctcgttct gttacggata
     2461 ctgcaccgcg agcatctaat aaattgaatg tatggctgca tttcaaaata taatcatacg
     2521 ctggatgaac aagattttca tcgatacagc gtttagcttc tttttcaaaa cggtcaaagt
     2581 tgcttagtag caagtcttgg tcacttactt caaaagaata tttggaatgt tcatattctg
     2641 gttgtttgaa gatctcgcca tatttcacac catcagccca ttccagatca tagacacttt
     2701 caacttcttg aatatatgaa gcaagacgtt ctagtccata agtgatctca gaagtaactg
     2761 gtttacaagg tagtcctccg acttgctgga agtacgtgaa ttgtgtgatc tccatcccat
     2821 caagccatac ttcccaaccc acaccggcac accccatcga tgggttctcc cagttgtctt
     2881 caacaaaacg aatatcgtgt tctaatggat caattcctaa aagacgcaag ctttctaaat
     2941 aaagctcttg gatattttca ggtgaaggtt tcattacgac ttgaaattga tgatgctgat
     3001 acaaacggtt tgggttttct ccataacgcc cgtcagctgg tcggcgggat ggctctacat
     3061 aagcagcatt ccatggttcc ggtccgattg cccgtaagaa tgtatatggg ctcatcgtac
     3121 ctgctccttt ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt
     3181 tttgtaatgt taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt
     3241 tcaattttta agcgttgctg tcaataagaa tacaggaaag aaaacgacaa aaagtcccta
     3301 tgtctgatta cagacatagg gacgaataat ttcgcggttc caccctactt ccgaataaag
     3361 attcggcagc ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc
     3421 ttccactctc ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta
     3481 ttcattttgc agttttcttg tttatataat agatcctctc agtgaacgat ctatttaatc
     3541 tacggttaat aatagacgga agtccctgta tttgtcaaat agttttgatt tttttgttgt
     3601 atctttcatt tattcttctt tttcaggtat ctctttttca ggcatatttt tctctggcgt
     3661 ctttaatacg tgtgcccaag acttcatttg atcaatgaac tttttgcttt ttagatgaat
     3721 accgacatat tcatcataca actggtcaat gaggtcacgg attgcctgct tcgtttcttc
     3781 tttcaagtgg attccactga tcttatcata agaaatagcc gaaaacattc gaacgaaata
     3841 aacagcgcga ggatctgcat gataacggtg ttcgtccatt ggccagtgtt tctcgcatat
     3901 cacaccgcca gatttggaag aatagtcaaa ttttcctttt gtttccccgc atacgacaca
     3961 gtgcgtccaa attggcgcga ttccgaagcg ttgcagtatt tgaacttcaa agatattcgt
     4021 gatgatttct gcatcatctc cttgatccat ccgctgcaga gcttgttgcg taaattggaa
     4081 taaatgcggg tcatattgat gatcttcgat tgctgcatct actagattta gcaaataggt
     4141 accataaccg ctcaaaaaga tatcttcttg gatgttccga aacgcatgga cgtctttgac
     4201 actgcttaaa aacgacaaac cttcacttcg aaaattaccg atatagactg cttcggtaaa
     4261 ggggagcaaa gccggagcta atggctgatt ttgccgatgg attcctttga tataaaacat
     4321 cattttgcca gaagattccg taaagatttt gactaattta tctttttctt tgtaattacg
     4381 gctagataaa atcaagcctt tcgattctgc aagctggctc atattctccc tcctaaaaaa
     4441 taaatctgga aaagcagatt ctgccgatcc agattattta tgtttcgatc ttccgccgtt
     4501 tttctcaacg tgcggatttt tatccagttt atttctatac ttaacctata agaagttcag
     4561 aatatcagta ttcgtctttt cggtagccaa agtcttgcag atagactttt ttgtctcgcc
     4621 agtctttttg gactttgacc caaagttcta gaaatacttt gtctcctaaa agattttcga
     4681 tatcctttct tgctttcgtt cctacttgct tcagcatttt tccgcctttg ccgataatga
     4741 ttcctttttg gctatctctt tcgacgatga tcgtggcctg gatatggatt ttatcattct
     4801 cgtttcgctt catcgaatca acaacgactg cgacagaatg aggcacttcg tctcgtgtta
     4861 aaaacaagac tttctcacgg atcaattcag aaacgataaa gtattcagga tgatcagtca
     4921 cttgatcatc tggaaaatat tgtgggccct ctggcatttc actgactaat acatccatta
     4981 agcgttcgac attgtttcct tctgtagcag agatcggaat cacttgtgca aaaggcatct
     5041 gcgtcgaata atcttcgatg attcccagca actcgtctgg atggatctta tcgattttat
     5101 tgataatcag ataaactgga gaatgcacgt tttttagacg ttcaataata aaatcgtctc
     5161 cccggccgcg tttttgatca gcactgatca taaataagac tgcatccact tctttcaacg
     5221 cactgtaagc tgcttctacc ataaaatctc ctagacgatg ctttggttta tggattcctg
     5281 gtgtatcgat aaaaatcagc tgagcttctg gtgtagtata tactccttgg attttatttc
     5341 tagttgtctg tgccttgtca ctcataatgg caattttttg acccacgatt cggttcaata
     5401 atgtggattt tcctacattc gggcggccga cgattgcgac aaaccctgat ttatgttctg
     5461 gcatatacgt tctccttttt aattaaacaa caaatgatat attttaggga caaataaaat
     5521 caacccaatg atgacagcaa aacaagcggt cactaaaact gctccagctg ccatatcttt
     5581 gatttttttc ccaatcggat gaaaatgaaa atccgtaaac atgtccacta cattttcaaa
     5641 aacagtattg atgatctcta ctatccaaac gagaaaaaca gctaaaagca gccataacca
     5701 ctctatccga tcaagctgga aaatcacgcc cataaataaa gcaaggatac cgaacgcaac
     5761 atgtttcctc atattgcgtt cttcctcaaa aacggttttg actccttgga cagcaaattc
     5821 cacagacatc aggaaatgtt tgtttttctc tacgttttta tcttttgagt ccataggcat
     5881 ccaagatctc tttctgtaaa ccaaacattt ctttctcatc ttctggtgtc atatggtcat
     5941 acccgttgat atgcaagaat ccatgtaccg ctaaaaagcc aagttcccga tcatatgaat
     6001 ggccgtaagc ttctgcttgt tcagcagcac gttcaataga aatcatgatg tctcctagta
     6061 cacgaggcaa aggattctct ccatcttcaa aaatcactgg gatttcgtct tcgccttctt
     6121 cttccagagc aaaactgatg acgtctgtcg gtgcatcttt tccacggtaa tcacgattga
     6181 ttacttggat tgccgcattg tccatgaaag taacagacat ttctgtttcc tctggcagct
     6241 ttaaataaga tgctgcaaat tgcagcaagt catcgatttc tttcattttc tcttctgaga
     6301 cttggtttgt ttcatccata aaggtaatat ccattataca tcgcttcttt ctattcttct
     6361 tgttcttcct ttatcttctc aacttctgtt ttcattttag gatatttgat ccgagaatgg
     6421 aaggtgctac ttaaaccgct gatcaatgat ttttccactt ttcggatttc tttcaaggtc
     6481 agtccactat cgtctaattg accatcggaa atacgctctt cgataagatt atggacaaaa
     6541 ctttcgattt tttcgctcgt cggatgatcc attgcccgta ctgcagcctc acaactgtca
     6601 gcaatattga cgatccctgc ttcacgactt tgcggttttg gtccaggata acggaattgc
     6661 tcttctgtta cttccggatt gcgttctttc gctttgatgt agaaataacg cattagcgtg
     6721 gttccatgat gctgtttgca aatatcgatc accatttgcg gcatattata atcttccaat
     6781 attttggctc catctgtcac atgtccaaag ataatatgtt tactgtcttc tggcaataag
     6841 aaattgtgcg ggttttccgc tcctgaaggc aagttctcaa cgaaaaagct agcatgtttg
     6901 atcttgccga tatcatgata atagcaagct actcgagtta gcaaagaacg tccgccgatt
     6961 tctgctacag cgttagcact tagattagct accatcatac tatgatgata agtacctggc
     7021 gcttcttcca gcaactgttt caataaagga tgatttggat tgctgagttc atttaataca
     7081 attacactgt catcattgac catcaattca atatatgggt gtagaccgat cgtcagcaag
     7141 aatgagaaga gtgcaccagt gaacccgcag acaagcatta accaagtcgt tgcatcacca
     7201 aagctcattc cttgatagac gatcaaaatg acatccatca tcacaggaaa aataatgacc
     7261 cacatggcag cagataatcc ttgttctgag atccgtttgc gtctgacaat cgttgccatg
     7321 agcccagaga aaacataaga catcaagata atcgttagaa agttcgtgcc aatcgaatca
     7381 taaaaaatga aaagtgcaga tactgcttgg aaaataccag ccatgatccc tgctcttcgg
     7441 ttcaagaaaa agcttaaaac aagtggtgca aaagcagcgg gataaaacag cgggatataa
     7501 gtagcttgtt ccgtttggaa taattggaaa aatttcatca gcactatgct gactgacata
     7561 gctgtcacat agaaaagcac gtattttgtt ctttctcctg cattttggaa ttgaagtgaa
     7621 ttataaatca gtacgccgat ttgaagcaaa acagctaaaa ccatggcaat gagtgggaaa
     7681 atcgaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc tgaagcatca
     7741 atttgatttc cttcgcgaac aatgatttct ccttggaaga tcatcactgg ttgtacagct
     7801 tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc attgactaca
     7861 atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac attcaaatac
     7921 tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg aacttgttta
     7981 ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ccgtgtgaat ttccgtttca
     8041 gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa cgcaacgttt
     8101 tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc tgtagtaggt
     8161 ttcgggatat tttcatcttc cttcgcctta tctaccttgt cattataaga tttgttgatg
     8221 gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt ctggtcatct
     8281 gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag ttttcttctt
     8341 tgttcggttt cttccgtgtt ctcgatcgtt ttattcgcac ggatactctc ttctgctact
     8401 tggccttctc tgaaatcatt ggccttttgt ttgacactac taaatgtaac gccgcagaca
     8461 atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt ttcttgtagt
     8521 ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat tcttgatgaa
     8581 gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga acaacgtcac
     8641 ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt tagtgttcgt tctgcatgaa
     8701 caagaccact tgtcgtccct cgaggcaagt ctatctgact tgtatcccca ttgacaatca
     8761 tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact gtcgtgtttt
     8821 gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg taagctaatg
     8881 gtgcgatttc gattacgcct cgttccatca atcggttcgt atgatccaat ccaaaaattt
     8941 ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc agatccccgg
     9001 gtagaaagcc taggttttcc cctgcctcta cagcgggccg cgtcaaaatg attttttgca
     9061 cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt ttccctgttc
     9121 cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg tatttctttt
     9181 gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc tcttcctcat
     9241 acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc gtcacgacat
     9301 ctggtgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga atgactgagg
     9361 cagccaagga aatctgggat tgttcaccaa tgatctgaat gatttctcca cgtgtatgga
     9421 tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca cctaaaagca
     9481 tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct tccgtcaaaa
     9541 atagacatcc cctttttttc gattcattct ttcctatttt accatactca gaagaaacta
     9601 tatagcaaat aagaaaaggt tgtaaaagag tcgtttaacc atacgtacca agaaacgtta
     9661 aaaaatagct gtttccattc gctaaaggtc tcgttttgat aaaaagaatc tgagaatgta
     9721 agttatatga aagtggagaa caaaatattc cgtttcattc agaggtattc tctaggtatt
     9781 tttcttgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt caaaaaaaac
     9841 aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa acagtaggct
     9901 attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac
     9961 gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct ccattatcag
    10021 gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa
    10081 aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc ttttttttat
    10141 attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat tgctgcaacg
    10201 tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag aaacaaaaac
    10261 ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt ttttcgggtc
    10321 aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact gggtcagtgc
    10381 tggcgtagga tttgccatct caggcgctct tttcccatta ctagctatct tagctgtagt
    10441 agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat ggtatgctgc
    10501 cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac cacggacagc
    10561 tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata attcattagg
    10621 tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg tccatcccac
    10681 acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat tattagcaat
    10741 catttttgtc gtttctttat tccacccaat gggaaatcta aatcaatctg ctgcagatac
    10801 ttaccaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg ttgatgcagt
    10861 agccttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga
    10921 taaaaaagtc acagaattaa cagcaaaagc tggtagcctg agcgttgcgc tagaagtcat
    10981 gatctatttt ggtcttgttc ttctcggtgc gatgagtttg aataagatgt ctttatcaga
    11041 aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact tcggcgctag
    11101 cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac tcgttgtttc
    11161 ttttgcacaa gatttccata aactgttccc taaagtcagt tatatgacat ggctgcggtt
    11221 aacgactttc gtctcttttg tcgttgcaaa tgctggtttg gacaatatca tccaatggtc
    11281 tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgattttgc tttccttaac
    11341 agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt ttgctgctgt
    11401 tcccgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac aaagagttgt
    11461 agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattgg gacttggctg
    11521 gatggtccca acgttacttg ggtatgctgg cagcttactc ttctattacg cctatcgcct
    11581 atcagggtat aaacaagaag caaatgaact accagaagaa taaccagatc aaacacaatt
    11641 taacataaga aatattaaac aggaaaaaaa cagctagatg aaagaatcct attctttcgt
    11701 ctagctattt tttagatctc ttttttacga gcacaaaaaa actgcggaca atgattgtcc
    11761 gcagtttaaa tgataaaatt aagattgtaa tagttctttc acaatggcat ttacttggtt
    11821 tccatctgct tttcctttga ctttaggcat gatcgcaccc atgactttgc caaattcttt
    11881 cattgatgaa gcaccagttt gcgtgattgc ttcttgaaca agttgtcgaa tttcttcgtc
    11941 ggaaagttgt tccggcatgt atttttcgac tatcacaatt tcacttttta ctttttcagc
    12001 taaatcgtca cgtcctgctt cttcaaactc atgcaaagaa tcacgtcgct gtttcatttc
    12061 gcgagacaac accgttaatt cttcttcttc tgtcaagtcg tgaccttctt taatctgctc
    12121 gttttgtata gatgacttga tcattcggat gacttgtaaa gattccttat ctttcgcttt
    12181 catcgctgtt ttcatgtcat cgtttaatct acttagtagt gacacactac caactccaat
    12241 tcatcacaga actagaattt tttgcgtttt ctagctgctt cagatttttt cttgcgtctt
    12301 acacttggtt tttcgtagaa ttcacgttta cgagattctt gtaaagtacc cgcttttgac
    12361 acggaacgtt tgaagcgacg aagagcatcg tcaagtgatt cgtttttgcg aacgactgtt
    12421 tttgacatgt taattccctc cctccgagct taaaaagtaa ccgtaattgt tattgaaata
    12481 tatctcaaaa aacaagactg tcctgtaaat attatatcta atgctgtttt gaccgtcaag
    12541 gaaatcttgc ttttttttaa aattatcaaa tgttatttta tttaaatcct tattgacaat
    12601 tttcacatct tccgaaaatc tcgaatctat gcccttcgat cgtacatcca ccgagctgtt
    12661 cttcgaaata attcattgga cacatatgga tttctttcgt tgttccgcac accgtacaga
    12721 taaagtgatg gtgatggccg atttcccctt gacagcaatg aaaacgaaac ttcatctctc
    12781 catttaaatc ggtttcttcc agtaaatcga tttctgcaaa atcatggaga tttcgataaa
    12841 tcgtatcgta acttactcct ttgaattcct gattcatata gtcaaaaact tctttagcgg
    12901 atacgtagcg attgcgcttc actaaataag ttaacaatgc ttctcttttt ttagtatatt
    12961 taaaaccgtt atttttcaat gtttctagtg attgttcgat caacgtctgt tttttcatga
    13021 cttattcctc caaatcaaaa tgattacgaa gtacacattc tagagtatgg tataataaac
    13081 ataaaaaaaa tgcaagtgag gttttatcca tgtcttccga ttctacagaa atcgttactt
    13141 tttttgtcat ctccgactct gctggagaaa ctgcgacaaa actagcgcaa gccactatgg
    13201 cccaatatcc tagcgttgag ttcaatttat tccgcagaac atttgtgaca gatgagaaaa
    13261 cattgatgca ggcactgcaa gatgcactag aagaaaaagc tcttgtcctg cacacattga
    13321 tcaatcaaga attgatcgag atgacgcgtt ctttttgtga agaacatcaa ttatttagtt
    13381 ttgatgtgat gactccgccg atatcagaaa tcgagcgctt aactggtatc aagcccattc
    13441 ggcaacctgg cgcccttcat ttattgaatg agaactattt caaacggatc aaagcaatgg
    13501 aatttgctgt aaaatacgat gacgggaaag atcctcgtgg gtttctagaa gcagatgtcg
    13561 ttttgctagg cgtatcccga acttccaaaa cgcctttgag cttgttttta gctaacaaaa
    13621 atttaaaagt tgccaatcta cctttgatcc cacaagcaca tattcccaaa cagctttggg
    13681 agatcgatcc taaaaaaatc gtgggactga cgaataaccc agatatttta aacaatatcc
    13741 gtaaagaacg gatgcgctct tacggattaa atcctgatac tgcttattcc gatatcgaaa
    13801 aaatacgagc tgaactcgat tttgcgaatg atctttatga aaaattgggc tgcgtagtaa
    13861 tcaatgtcgc ttctttatct attgaagaaa cagcttcgct tattttgaat gctttagacc
    13921 tagaagacca tagttattat ggaactgaaa ccaccgaaga aacagactaa caatttttct
    13981 ttaaaaaaac tggactgtgt cagcgaagaa gattcgttga cacagtccag tttgtctcta
    14041 tttgcaccta tcgttattga tgttccacat gtttgagtgt ctggttcaaa atatccagta
    14101 cgtgagaatc atcaagacta tacaaaatcg cttttccctc acgatgggac ttgacgacat
    14161 ggttttctct taataaacgc agttgatgcg atacagcaga ttgttccatt tccactgctt
    14221 ggatgattgc tgtcacattt tgttccccgt ctttcagata cagcagaatt ttgagacgag
    14281 tggggtcact caacattttg aatagttggc tgactttctc tacttttgtt ttttgcgtgt
    14341 ctggcaatcc attcacctcc aacttgaaaa aagactgact tctaaagttt accacttttt
    14401 ccatcagtct caagtttttt tattcttctg tcatcttttg gataatcgtt tcataagcgg
    14461 ctactttttc agctgcgtct tctgaagagt ttccttttac accgatataa aatttgattt
    14521 tcggctcagt tcctgaagga cggatagcga tccagccatt ttcttccagc gtatacttca
    14581 atacgtcaga tggcggtgtc gttagttttt ctacgtttcc ttcacggtct gttctcgtca
    14641 actctttgaa gtcttctgtt tggatgactt ttgctccggc aaattctgtt ggtgcttctt
    14701 cacggaattt ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg ctcattgtca
    14761 cagaaatcgt tttttctgcg tagtagccat aggtttcaaa aatcgtttgc aagccatcat
    14821 acaacgtttt gccttgtttt ttatagtaag ctgctacttc tgctaataag agcaatgctt
    14881 ggatcgcgtc tttgtcgcga acgaaaggtt taactaaata accgtaactt tcttcgaatc
    14941 cgaacataaa agtatgggaa tgatcttctt caaattgttg aattttttca gcaatgaatt
    15001 tgaatcctgt caatacattg aacatggtag ccccatagtg ttccgcaatt gctgtaggta
    15061 attcgctaga aacgatcgat ttcaataccg ctgcgttttc aggtaaagtg cctcgcgatt
    15121 ggtgtgcttc caaaatatat tgagcaaaca gtgctccgat ttggtttccg gtcaatactt
    15181 cataggagcc atttggcaaa cgaacggctg cacctaaacg gtctgcatca ggatctgttg
    15241 caatcagcaa atcagcgcct tctttctcgc caagtcgtat cgcatactca aaagcagagt
    15301 gttcttctgg atttggtgat ttgactgtag tgaaatctgg gtctgcgaca gcttgttctg
    15361 gaactagtac gaattgttcg aatccagctt gtttcaacgc acgttcgccc aacatttttc
    15421 ctgttccgtg aagtggtgtg tacacaagtt tcaaatcttt ccccattgtt tccaccaatt
    15481 catgatcaat cgttactgct ttgatttctt ctagataagc ggcatccact tcttctccga
    15541 tgatcgtgat caatccgcta tgttcagctt cttcgtcaga taatacttca acttttaacg
    15601 gattgctgac ttcacggaca tatttcgtca atgcatctgc gtctgcagga ggcatttgtc
    15661 ccccatcttc tccgtagact ttatatccgt tgtaagctgc tggattgtgg gatgcggtta
    15721 ccatgattcc cgcaaacgtc ttcaaatgac ggactgcaaa agaaagctct ggtgttggac
    15781 gtagactttc gaatacataa gatgggatat tgtgatgtgc taatgttttg gctgcttcca
    15841 tggcaaattc tggtgattga tggcgtgagt cataagcgat agcgacacct cggcgttttg
    15901 tttcttcgcc ttgtgtatcc atgaaacgtg caagtccttc tgttgcttgt cttaccgtat
    15961 agatgttcat tcgattgatc ccaggcccta agattccccg catgcctgca gtaccgaatt
    16021 ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc ttcacccaga tcatttaatt
    16081 gtttttttaa gttttcttct aaattttctt catgtaccca ttgttcatac acttgttccc
    16141 aagacatgaa aataacacct ctttctgaaa tatggttctt aataagtata gcattcactt
    16201 attagatatg aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat tcttttatca
    16261 agcacggttg caagtattcg cataaatcgg attcagtaga tttcacgcga aaaaaaagag
    16321 taaaagaaca attaaaccgt tcttccactc tttttaaact tatgcagttg tttcttctaa
    16381 agattccaag tatttataca cttgttgtcc agcgatccca ccatcaccaa cagcagtagt
    16441 gatttgtcgc aaatcttttt cacgaacatc tccgatggca aaaacgcctg gtatattcgt
    16501 cttcatttcg ttgtctgttg gaatccagcc agcttcgttc gtcaaccctg cttctctaaa
    16561 tggtgcagtt aatggatcca gtccaacata gatgaatact ccgtctgctg ccaaatcaaa
    16621 ctcattgccg gttttgacat tacgtgcttt cacacctgtc actaccattt cattcccaac
    16681 gatttcgtca acgactgtat cccacataaa ggaaattttt tcattcgcaa atgcacggtc
    16741 ttggatgatc ttttgtgcac gtaattcatc tctacggtgg acgatgacta cttcagaagc
    16801 aaattgtgtc aaatagatgg cttcttctac tgctgagtct cctcctccga tcactactag
    16861 acgtttgtta cggaaaaacg caccatcaca aactgcacag taagaaacgc cacgtccagc
    16921 aaattcttcc tcgccaggta cgcctaattt acggtggata cagcctgttg cgataatgac
    16981 tgtttttcct tcgtaagttt tatcttcaca aataacttct ttgtggctgc cgtgatcctt
    17041 gatatcttgc acgattccat aagcattttc tgtcccaaat cttgaaacac cttcatacat
    17101 cttataagct agatcaggac ccattattga atcgaaacca ggatagtttt cgatttctgc
    17161 cgtattgttc atttggccgc caggggcgcc ttgttcgatc atcaatacgg ataagtttga
    17221 acgtgaagca tataaagcag cagtcatccc agcaggacct gcgccaataa cgattacatc
    17281 atacatatgt agattctcct ttacttaatc aagtaagcat actttacagc caatgcgaaa
    17341 aaaagtctat tgttctgctt gcgtttctcg aatagcttta ttatactcga aaaatccgct
    17401 gaaaaacaat agacttttat aaatcattga tcaatcattc gaaagatttt ctccatttga
    17461 atcaattact tttttatacc agttgaatga tttcttcttc gaacgggcta atgtgccatt
    17521 tccttcgttg tcacgatcga cataaatgaa tccgtacctt ttcttcatct cacctgtacc
    17581 agctgatact aaatcaatac atccccaagt cgtatagcct aatagatcga ctccatctaa
    17641 ttctactgca tctttcattg cctggatatg tgctgccaga tagtcgattc gatagtcatc
    17701 ttccacataa ccattctcat cgggcgtatc gactgcacct aagccgtttt ctacaataaa
    17761 tagtggtttt tgataacgat cataaatatc attcatcgtt attctcaagc ctaatggatc
    17821 aatttgccaa ccccattcac ttgcttccag atatggattt ttgacagaag caaagatatt
    17881 tcctgcagtc tgttccaaga gttcgttgtc agtagtcgaa actcgagaag aataataaga
    17941 aaaggagata aaatcaacag tatgttcttt cagcaatgcg gcatccccag gttccatttt
    18001 gatctgaatg ccatttcgtt ccatttcttt caatgcataa gcaggatatt ctcctcgtga
    18061 ctggacatca atgaagaaat aattttctcg atctgatttt ctagaagccc aaacatcttc
    18121 aggattgcaa gtgtatggat aatatgctcc tgctgccagc atacagccta ctttgttctc
    18181 cggatctact tcatgagcaa tcttcgttgc gatggcactt gcaaccaact catgatgtgc
    18241 cgcttgatat ttgatttgtt cttcattttc tccttcttca aaatacagcc ctgcgcccat
    18301 gaatggtgca tgaaggatca tattgatctc attgaatgtc aaccaatatt tgacaagtcc
    18361 tttatagcga ttgaagatga ctcggcacag attttcataa aaaccaacga gttctctgga
    18421 acgccacgca ccatattctt tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac
    18481 aagcggctcg atcccgtact tttgacattc tttaaagaca tcctcataaa atttcaaacc
    18541 agattcattt ggctcttttt catcgcccat tggaaaaata cgactccaag caatggacat
    18601 ccgataagtc ttgaacccca tttctgcaaa taaagcaata tcttccttat aatgatgata
    18661 catatcgatc gcaatttttg ctggataaaa atgattttca tcaaattcaa acattttctt
    18721 ttttccagta atgacaggaa aacggtcttc tccgattggc acaacatcta cgttcgctaa
    18781 cccacgtccg ccttcatcat aaccgccctc gcattgattg gctgctgtag ctccgcccca
    18841 taaaaaatct tttctgaatc ccatcatact tcctccatta catgtgtatt gcttgataat
    18901 tctcttgcca gttcatcttg gacttcgatt ccttctgctt ttaaccggct gattgtttcc
    18961 ttgaattgcg gtaaatactg ctgatgtgca ataaacaact cattcatgat ccgttttgct
    19021 gtctttcctg atcgtataag cggattgatc gtaaacgctt gtaaagctgt tccataatca
    19081 ccagtcacag ctgcttcgat cgtcagcaat tccatattct tcattacttg caaccagcct
    19141 ctttcagcag caggaaggga accaaacgct acatttcgtg cgccttgtgc gccaacatag
    19201 gcagtgactt ctactacaca atctgccggc agatcaggaa cagctccatc attctttgta
    19261 gataccacga tctgtgtttc tttatttcca tagatcgaag caatcgtttc acaggcagca
    19321 tctgaataat aagcaccacc tcgtttttgc aattgttcag gtttatgatc caattctgga
    19381 tctttgtata attcaaacag ctctgcttct gtttctttca cttgctgcgc tcttgtacca
    19441 atcgtttgat attcttctaa agcatgttcc agcatttctt cttcacggta gtaatagcgg
    19501 tggtagccac aagggatcat gttcatttgt tttaattgtt ctttgaaaaa cgggatatca
    19561 aagatatttt tcggcaatcc attgtcttca tcgtataaac gatcaatcaa ttctgccgtg
    19621 cggtcatttc ctttattatc tgctactttg tgccaatgga agtgatttag accggcaaac
    19681 ttgtagatca gctcttcttt tttcttgcca agcattgttg gttctgtcat catcgccatt
    19741 actggcacat tgcataatcc tatcacttta tcccatttgc catatctgat aactgcttct
    19801 gtcaccatcc cgcttgggtt cgcgaaattg atcagccaag catttggaca taaacgcttc
    19861 atgtcttcaa cgattgccaa aaccactgga acggtacgaa aagctttgaa tatcccgcca
    19921 gcaccatttg tttcttgccc aatcatccca taataactag ggatccgctc gtctttgatt
    19981 cgtgcatcta attgaccgac acgaaactga gtcgtaacaa aatccgcatc tttcaatgct
    20041 tcttcccgat ccagagttgc atagattttg acttcatacg gagaagcatc ccacatacgc
    20101 tgagacatct gactgacgat ctcaagcttt tcttttcctt cttctacatc tacgagccag
    20161 atttctcgta tcggtaattc ctcatatctt ttgataaatc cttccatcaa ctcgggggtg
    20221 taactgctgc ctccaccaat cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt
    20281 tactttttac acttttatta tcttttttgt aaacaaaaca aacaatagaa attcgtttct
    20341 ttccagtaac atttacaaaa gttagtgttt tctactggat ttttgtaaat atcttgtcaa
    20401 aaaatgtctt tattaagcta tataacataa atccgtttac aaatcatttt caaaaaagtt
    20461 ataatttacg taaaaaaaca agaagaaggt atctacatgt tattacgtga aaaaatcaaa
    20521 gaaatctcct tttctccttc agaaaatgaa gtgattcagt ttctcttaag cgattctcca
    20581 gcacttggcg aaatgaccat ccaagagatt gctgagcgat gttatgtcca cccgtccaca
    20641 ctcatccgtg tagcaaaaaa aatgggtttt gatggctggg tcagttttcg taatgctttt
    20701 ttagctgaaa cagcctatct tgaagggaat tttcaagacg tagatgcgaa tcttcctttt
    20761 tccaccacag acggtattat gacgatcgca aataaaatgg cttctttaga acagatgacg
    20821 attcaagata cactttctct cattcatcat gaccaactgc aaaaagccaa acaactaatg
    20881 cttcaagcaa aaaaaatcgt gattttcgcc agtaactcga atactttgat ttctcaagat
    20941 ttcatgctga aaatgaagcg catcaaaatg gatgtcacga ttgtcaccac ttttggtgaa
    21001 tcgttctatg aagcgtacaa ttgcacgagt gatatgtgcg ggattctgat ttcttatacg
    21061 ggtgaaaaca atgtgatcct tcgtaccgca gaaatattga aacaaaatca tgttccaatt
    21121 ttagctttga ccagtatcgg tgattcaacg ctatcttcaa tgagtacagc cgctttgcga
    21181 cttacgacac gcgaacgact gtattcgaaa attggtaatt ttacgatcaa tacatcgatt
    21241 tgttatcttc tagacgtgtt atatagttgt gtttttgcag aaaattatca acagaattta
    21301 gtccatctaa tttctgtagg acaaaaattt gataaacgcc ctatcagctc agccatcatg
    21361 aaagagcctt tcaattttga aaataaagaa gtggactaaa aagaataatt aagcctagtc
    21421 cattctttca cctaaccgtt caggtcagtg cggtgataag tgcatcgtct ggcccgaccg
    21481 tctccccttg attttccaaa atatccaaat aatccgtgta attggtgacg atcacaggtg
    21541 tttctaaact gtatccttcc tcttcgattt tctttttgtc gaagcggagc aacagatcgc
    21601 cttgtttcac tctttctcct tgctgcacca agctttcaaa atatttccct tccaactgaa
    21661 cagtatccaa tccgacatga atcagcaatt ccagacctgc ttcagaaacc attccgattg
    21721 catgtttcgt cgggaacagg gtcatgactg ttccgtcaaa aggggcacgt acttctcctt
    21781 tttcagggta gatcaagatt cctcgaccta gtgttccttg tgcaaaagct gcatcctttg
    21841 ctgcattcag tggagcgatt tgtccttgta tcggtgtttt tacaatttct ttacctgatc
    21901 ttttctgtct atcatctgtt tcacctgctt cctccttcca aaagaaaaaa gtcagcgcaa
    21961 aggataccac catcgcaatg gcgattccaa tcaatacatt aatcacatcc gttaaatcac
    22021 catctggtct aatatagttg ggaattgtaa agatgcctaa tccacctgga ttccaagaag
    22081 ttaaattcat taatccacta tacgcgcccc ctaacgcacc accgacacag gtaaaaataa
    22141 atattttttt acgtggcaat gtaaacccat aaatagctgg ctctgtaatc cccacaatac
    22201 cagaaacaat ggctggtatt gcgattgctt tgtcttttgg gtttctaagc ttaaagtaga
    22261 gtgcagtaag tgctgcagtt tgtccaaaag acactactgc aacaggtgtc atgatatttt
    22321 gccaaccatt cacagaaaat tgcatcaatc ccattggtac gattgcccaa tggagaccaa
    22381 acatcactag aatttgccag aagaagccta cgattgctcc ataaataact ggcgaaagtg
    22441 ctaaaagtgt ttcaaatcca gccattaaaa tatcagttgc gaatgtgaat gctggtccaa
    22501 cgagtaaaaa acctaagggc attgtaataa gaacggtgaa gaatggaacg aagaaattcg
    22561 cgatcatctc aggtactaac tttttaattg gtttctgaat ttggctagct aacaacacaa
    22621 taaagataat gggaatgaca gaagaagcgt agttacgcgc aatccaggga atgccgaata
    22681 ctgtttgata aataggtgct tcaaaaatag tgccagaaaa tatggtagtc agtggtgctt
    22741 ctgctgcacc actcaaagct tccagcgaca agctaggatt cattaatgct gccccaagca
    22801 acatccctac gaattcattt actttaaatt tacgggcagc tgttaaaccg ataataacag
    22861 gcataaattg gaatacagaa tcaccgatat tatcaaacat aacaaatgtg ccagaaccgc
    22921 ggtcaaatgc gcctaagaag acaagtaatg ctgtcagtcc acgaatcatc ccaccagcag
    22981 ctagaatagc aaggaatggc tggaaacagc ccgatattcc atcaataagc atatcaaacg
    23041 gtctcatttt ttctgttgaa acagcctcat caaaagtaag gtttcctgct tttataacct
    23101 cttcaaacac gactggcaca tgattcccga tgacaacttg gtactgtcct cccgctcgca
    23161 ttaccgtcac aacaccgtcc atatttttca agatctcatc atttgctttt tcttcgtttt
    23221 ttaatttgaa acgaagcctt gtgatacaat gtgtcaaacc attgatattt tccttcccgc
    23281 cgacattttc tacaattttg ctcgctaatt cttgatattt tcccatttct attccctcca
    23341 atttattagt ggaaggttta gccaactgaa tgtcactacc cttatttcat gcgttcttgt
    23401 taatttatcc tccttttttt atttatttac aacgattctc atgcactaaa cgcgcaatat
    23461 ggatcgttaa atacattttt tcgtccgtgg acatctggta gttgaatatc tcggctaaaa
    23521 agtctgctat tttctcaacg caaccaaacg tatccttata ttttttttga ataacctggt
    23581 acaattcctc agaaacttct cccacttctg atttttctcc atctaggacg cgtgtcagaa
    23641 aaaaacgtag gtgtgtgacg aatcgaaaaa aatggactga atcttcatca aatgtaatct
    23701 tatagtaata gcggacgata tttagaatat cctgtattgt tgtagtaaac aggtaggtat
    23761 tgtcttgttg gctttgaatg ccttgtgcat tgactaaatg caatgcgata aatccggctt
    23821 catcttctga taacttgatg tggtatttat ccgagatttt cttcactgtt tttttcccga
    23881 tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt ggtacaaata
    23941 ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca atatacaccg
    24001 aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca caagaaattt
    24061 ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttgtgaa agtccgtcat
    24121 ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc actttctttt
    24181 tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatcc ctggttatta
    24241 cgacgttgtt attcagtact ttctcaatca gcatgtttta ctcctttcca tgatatatag
    24301 ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca tgggagactc
    24361 atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc tttatattat
    24421 cgaaagcaag cacttttgtc aacgctttct tagcggctat tgggcacttc tgactagctg
    24481 tcgtgatttt ttttaaatct ggtaattacg acactaacgc tcacagatga agaatctggg
    24541 aagctctgtt ttattttcac tctagcgctt aggtcaaaat gctactatgc tactgtccca
    24601 acttctaata tacggtgttc cagtaccagc ttatcggtga taagccaaat ttatgcaaaa
    24661 atctggaaag ccttttttac aatttttttc ttatcaccta aagctgaacg gagccctgtc
    24721 acaaccactt tttacacacc actgctttgc agtttgtaca tatctgcata caaaccattt
    24781 tgcgctagaa gtgtttcatg attcccacgt tctactatat gacctttgtc taaaactaaa
    24841 atcaattctg catcccgaat cgtggacagc cgatgggcaa tcgcaatcgt tgtccgtcct
    24901 tgtcgcattt ttgtcaatcc ttcttggatc aatccttctg tttcagtatc gatatttgct
    24961 gtcgcttcat ccaatactag tattttagga tctgtcacga ttgttcgcgc aaaagtgatc
    25021 agctgtcgtt gtccgctaga atagctggca ccgtgttcga tcacttttga gtgatatcct
    25081 tttggtaatt catcgatgaa cttgtttgct tgaacaaact cagctgcatg gcgaatctgt
    25141 tcgtctgtga tagttggatt catcatccgg atatttcctg cgatatctcc ataaaacaag
    25201 aaggcatctt gcaagaccaa tcccattttc ttacgtaact cttccattgg atattctcga
    25261 atgtcatgat catcaatcaa gatctggcct tcataaaatt cgtaaaaccg catcaatacg
    25321 ttgatgatcg agctttttcc gcttccggtg tgccccacta atgcaacggt ctctcccgga
    25381 ttagcgacaa aagagatatt gttcaataca ttgtgctctc catcataaga aaagcttaca
    25441 ttgcgaaact cgatcttacc tgaagtaatc gcttgatctg ctccaggatt ttgttgcggt
    25501 gcatattctt ctgtatccat gattttcaaa atacgactac cggcaaccat tccatctgta
    25561 aaggtgctca aaaagtccat catttgagac attggattaa aaaatccctg gacataggtc
    25621 gtaaatgcat agatcagtcc aacttctacg ggagaatgca gcgcatcgat cccaaacaaa
    25681 gtcaacgcca acgcaatcgc caatgcatat agaaaattga tgatcggact taacagaatc
    25741 gaatttgttt tgatcatcgc atagcgcgtg tctaaatact cgttgttcac ttgttcaaat
    25801 tctttagcta aacgagcttc ttggcgaaac tgctggataa tacgcatgcc ggaaatcgat
    25861 tcattcaatt tcgtgttcag ctgacttaat ttttcccgca tattccggta aatacgagaa
    25921 ctgaatttct ggtaatacca gatgacgatt cctaaaatcg gcaagaacac caaagtccac
    25981 aaggcaattt ctccattgat ttgaaacatg gcaatgaagg aagaaacgac tgcaaagatc
    26041 ccagtcaata ccatcaaaaa gacataccaa aattcaaata gcgtttctgt gtcatttgtc
    26101 actcgagaaa cagtcgatcc tgccggtgtc tgatcaaaat aacgcatacc tagtgtatgc
    26161 agcttttcaa ataatcttac ccgaacatac tgataggtct tcaatgaagc catggaatac
    26221 aaataccatt ggaaaaacca aatgatactt ttgactatga ctccaaacaa gtacatacca
    26281 gcaaaaaaca aaataacttg tgtcgtagcc gactgtttag ccagatgatc atccataaaa
    26341 gtttggatga tccttggcaa taggacattg atcacagaca gggcaaacgc aaataagata
    26401 gcaacgatga acgttcttct aaaaggtttg gcgaacttca tcagtcgttt gatgatcttc
    26461 ttctgttctt taagtggaat cgattttgtc cattccgatt gataattttc atccatcagg
    26521 cctcgcctcc ttctatcttc gcttctaatt tctgtttctc ccacattcgt tcataccagc
    26581 cgcctaatgc aagcagctca tgatgggttc ctcgttcaat gattttccct tgatcgacaa
    26641 caatgatttc ttttgcatgc atcacgctgc ttaaacggtg agctgcaatg atggttgttt
    26701 tctcttttct tatttctttt aaattatgca agatagcttc ttctgttttg gcatctactg
    26761 cagaaagtgc atcatctaag atcagcaatt caggattttt gatcaatgct cgagcaatcg
    26821 aaatcctttg tttctgtccg ccagataaag agactccacg ttctccaact aaggtatcat
    26881 aaccttttgg taattccttg atttcattgt tgataaatgc taaagaagcc gcatgctcta
    26941 cttcttcttc tgaaagtttt ggattagaaa aacggatatt atctcgaatg gtcatcgaaa
    27001 acaagaagtg atcttgaggc acatagccga ttgatcctaa taaagcattc aatgaatatt
    27061 ctttgatcga acatccgcca aattgaattt ttccttgata atcatcgtat tcacgcatca
    27121 gtaatttgat gattgtcgtt tttcccgcac cagttttccc aacaatacct aatgtttctc
    27181 cttcttggat cgcaaatttg atttgttcta atgctggtga ttcttctttc ggatagttaa
    27241 aagaggaaat agctacattc agctcgcctt ttgccggagt ctgcactgca cccagttgtt
    27301 ccacgatatg cgtcttttca tgcaaaagtt cattgacgcg atcataagaa gcatttcctc
    27361 tttccaatac gttgaacaat cgaccaatcg caaacatcgg ccaaacaagc ataccgatat
    27421 agctaataaa tgagaccatc tgcccgattg agatcgtgcc ttccaagaca tactttccac
    27481 ctaaaataat tgttgcaaca taagacagac cgataatcaa ggtaatgaat ggatcaaata
    27541 aggagtctaa aaaattgact cgtttgtttt taataattgc atcttcaatc ttttcttcaa
    27601 aatccgcgag atcttctttt tcttgtccaa atgttttaat gactttgatc ccagtaatac
    27661 tttcttgtgt cttatcattg atactagaaa aagcttcttg cgaatcacga aaagcatcgt
    27721 ggagttttga ccccagcact cgtgaagtaa cggctagtaa aggcagagga atcagcgcaa
    27781 tcagtgttaa gcgccaatca ataaacaaaa tcatcgcaat gatcgttgtt ccgcccgtga
    27841 tcactgaatc tgcaaaggtc aagataccag ctcctgctac attttggatt gcattcaaat
    27901 cattcgttgc atgagccatc aaatctccag tccgatattt ttggtaaaag agattgtcca
    27961 tttctgtaaa atgtttgaat agctgctgcc gaagttcttt ctccaaccga gctgcacttc
    28021 cccaaatatt cgtccgccag atataacgaa aaagatattg taagagagct gcaaggatca
    28081 ataataagat ccaccaggaa atgatcttta ttgataaatt tttatcggcg atctcatcaa
    28141 tgatgatccc aatcactttt ggaggagcta actgtaccaa ggccactgcg aatagggaga
    28201 aaatcccaat taaataatgt ttcttttcct gtcgaaaaaa ccaacctaat tttctaaaaa
    28261 tcgacatttt actcctcctt tgtctttatc aagagcatag gattgtctgt gcttgtcttt
    28321 tttgctgaat agtctgaata gtctaacaaa ttacgaaata aaaaacggac tgaaaaaatc
    28381 agcccgcaaa aagtctttgg gtcagaacct attttttccc ttggttcttc atttgctgca
    28441 tcatctggcg aattttcttc tcagatggtt tttgccccat tgacatcatc attgatcgca
    28501 acatgtcctc attaacggga ggattttttt ctaaatagtc tttcatatat ttacgcgcta
    28561 ggaaaaagcc acctactgcg ccgattagca aagcaataat agcaataagt acgacaatac
    28621 ctgttgatac catataaatc tctcctttct atttccgaat tgctctcaat gatttatttt
    28681 actaaagttt attgaaaaaa gaaaggcgta ttctttagtt tcatgcaaaa aatagtggtt
    28741 tttttagaga agaacaaaat ttctttcatt aaccagcaag acacgccacg aaaaaaaatt
    28801 aggcatacca aatctttttc tttaattttt tctttctgag gtgtagaatg taatcgtaga
    28861 taaataggaa aggtggtttt ttcttgaata atcaatccaa caaacaattt cataagcatc
    28921 gacttattga acatacaaat ggtttatctt tagctgaagt aaatggaaca gtggatgtac
    28981 ccaagggaaa aagttttttt aaaacacttc ttgcttactc tggaccaggt gcattagtag
    29041 cagttggtta tatggatcca ggtaactggt ccacttcgat cactggtgga caaaatttcc
    29101 aatatttatt gatgtcagtt attttgatgt ctagtctgat tgctatgctt ttacaatata
    29161 tggcagcaaa attaggaatc gtgacccaaa tggatttggc ccaagcaatc cgagcacgga
    29221 caagtaagac attgggtgtc attttatgga tcttgactga attagccatt atggcaacag
    29281 acatcgcaga agtcattggt gcagcaatcg ccctctacct gctttttgat attccattgg
    29341 taattgccgt atttatcact gtgttagatg ttttcgtcct tttattgctg acaaaagtcg
    29401 ggtttcgtaa aatcgaagct ttggttgtct gcttgatttt cgtcatttta ttcgttttcg
    29461 tctatcaagt tggcttgtct aaccctgatt ggcacagttt gtttgccgga tttatcccta
    29521 acagtcacac attctcagac tcaccggtca tcggcggcga aactccgctg actggagctt
    29581 tagggattat tggtgcaaca gtcatgccgc ataacttata tcttcattca gcagtttctc
    29641 aaactcggaa gatcgatcac caagacgaag aagatgtagc acaagcggta cgcttttcta
    29701 cttgggattc taatatccag ttgtcaatgg cttttctagt caatgcactg ctattgatca
    29761 tgggtgtggc tgtattcaaa accggttctg tccaagatcc ttcctttttt ggtctgtatg
    29821 aagctttatc tgatccatca gctatgagca atggtatctt gattgctgta gcaaaatctg
    29881 gtatcttgtc tactttattt gctgtagcac ttcttgcttc tggccagaac tctactatta
    29941 ctggtacgtt gaccggtcaa gtcatcatgg aaggcttcat ccatatgcgt atcccaattt
    30001 ggctaagaag actagtcaca cgattgcttt ctgtgattcc tgtattgatt tgtgttttgt
    30061 ttacaagtac aaaaggaacg atcgaagaac ataccgcttt gaataacttg atgaatgaat
    30121 cccaagtttt tctggctttt gcattgccat tctcgatgat tcctttactg atgatgacca
    30181 atagccaagc ggagatgggg agccgattca aaaattcatt tgttgtaaaa ctacttggct
    30241 ggttctctgt gattagtctg acttacttga atttacgagg attaccaggt caaatcgaaa
    30301 gtttcttcgg cgagcaagcc acctcagctc aactagcatt ggctgatcaa atcgcttatg
    30361 taatcattgt tcttgtcctt ctcctgttgg catggacgat tggtgattta tataaaggaa
    30421 acaaaaaata tgaacagcgt ttgcaggaaa cgatagaaga aatcgagtag aaactgattc
    30481 ttttacacaa aggaggttgt gtcagaagtg ggcagcttca agaaataaga aggaatttaa
    30541 gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tccgaagaag ttacttctgg
    30601 aacaccgtgt atcagaggtt gtgaaaaagc tgccttgcat caagtaataa gaacagccaa
    30661 gtaaaaacag ctttttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
    30721 tgagcaccgt ttattcggaa aaaggaactg cgacaagact tttgtcacag ttccctttcc
    30781 tacgattagt caagtaaaag gtgtaagaaa aatagctcat aaacgaaagc gacaaaatca
    30841 a
//
LOCUS       DACCWV010000018        29130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCWV010000018 DACCWV010000000
VERSION     DACCWV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.018"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(90..1385)
                     /gene="purB"
                     /locus_tag="IVW84_000737"
     CDS             complement(90..1385)
                     /gene="purB"
                     /locus_tag="IVW84_000737"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7916955.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1398..2531)
                     /gene="purK"
                     /locus_tag="IVW84_000738"
     CDS             complement(1398..2531)
                     /gene="purK"
                     /locus_tag="IVW84_000738"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7916956.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2515..3003)
                     /gene="purE"
                     /locus_tag="IVW84_000739"
     CDS             complement(2515..3003)
                     /gene="purE"
                     /locus_tag="IVW84_000739"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7916957.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3419..4726)
                     /locus_tag="IVW84_000740"
     CDS             complement(3419..4726)
                     /locus_tag="IVW84_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7916958.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4729..5310)
                     /locus_tag="IVW84_000741"
     CDS             complement(4729..5310)
                     /locus_tag="IVW84_000741"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7916959.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5426..5523)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5882..7174)
                     /locus_tag="IVW84_000742"
     CDS             complement(5882..7174)
                     /locus_tag="IVW84_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7916960.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7171..7793)
                     /locus_tag="IVW84_000743"
                     /pseudo
     CDS             complement(7171..7793)
                     /locus_tag="IVW84_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7796..8632)
                     /locus_tag="IVW84_000744"
     CDS             complement(7796..8632)
                     /locus_tag="IVW84_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7916961.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8649..9536)
                     /locus_tag="IVW84_000745"
     CDS             complement(8649..9536)
                     /locus_tag="IVW84_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916962.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9552..10835)
                     /locus_tag="IVW84_000746"
     CDS             complement(9552..10835)
                     /locus_tag="IVW84_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7916963.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11518..12201)
                     /locus_tag="IVW84_000747"
     CDS             complement(11518..12201)
                     /locus_tag="IVW84_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7916964.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12219..12836)
                     /locus_tag="IVW84_000748"
     CDS             complement(12219..12836)
                     /locus_tag="IVW84_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7916965.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12846..13670)
                     /locus_tag="IVW84_000749"
     CDS             complement(12846..13670)
                     /locus_tag="IVW84_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7916966.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13663..14223)
                     /locus_tag="IVW84_000750"
     CDS             complement(13663..14223)
                     /locus_tag="IVW84_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916967.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14397..15353
                     /locus_tag="IVW84_000751"
     CDS             14397..15353
                     /locus_tag="IVW84_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7916968.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15658..16614)
                     /locus_tag="IVW84_000752"
     CDS             complement(15658..16614)
                     /locus_tag="IVW84_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7916969.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16718..17320
                     /locus_tag="IVW84_000753"
     CDS             16718..17320
                     /locus_tag="IVW84_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7916970.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17408..18907)
                     /locus_tag="IVW84_000754"
     CDS             complement(17408..18907)
                     /locus_tag="IVW84_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7916971.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18923..19447)
                     /locus_tag="IVW84_000755"
     CDS             complement(18923..19447)
                     /locus_tag="IVW84_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916972.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19745..20494
                     /locus_tag="IVW84_000756"
     CDS             19745..20494
                     /locus_tag="IVW84_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7916973.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20485..21270
                     /locus_tag="IVW84_000757"
     CDS             20485..21270
                     /locus_tag="IVW84_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7916974.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21239..21955
                     /locus_tag="IVW84_000758"
     CDS             21239..21955
                     /locus_tag="IVW84_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7916975.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22057..23076)
                     /locus_tag="IVW84_000759"
     CDS             complement(22057..23076)
                     /locus_tag="IVW84_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7916976.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23095..24153)
                     /locus_tag="IVW84_000760"
     CDS             complement(23095..24153)
                     /locus_tag="IVW84_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7916977.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24374..25522)
                     /gene="nagA"
                     /locus_tag="IVW84_000761"
     CDS             complement(24374..25522)
                     /gene="nagA"
                     /locus_tag="IVW84_000761"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7916978.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25598..26434)
                     /locus_tag="IVW84_000762"
     CDS             complement(25598..26434)
                     /locus_tag="IVW84_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7916979.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26618..27835)
                     /locus_tag="IVW84_000763"
     CDS             complement(26618..27835)
                     /locus_tag="IVW84_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7916980.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28218..28301
                     /locus_tag="IVW84_000764"
     tRNA            28218..28301
                     /locus_tag="IVW84_000764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28252..28254,aa:Leu,seq:gag)
     gene            complement(28541..>29130)
                     /locus_tag="IVW84_000765"
     CDS             complement(28541..>29130)
                     /locus_tag="IVW84_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916981.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8677 a         6234 c         5008 g         9211 t
ORIGIN      
        1 ctaaaagaag cagaaccgct aacggatctg cttctttttt ggttattttt gctattctat
       61 tttaattgaa ttttgcaact cactcttctt tattccaaac ctactcgttt gaatatcacg
      121 tcaacgtttt tcaaatgatg atgataatca aaggcatcgt ctatttcttc tttagataat
      181 accgatgtga tggttggatc ttcttctaac agcgggcgga actgcacttg gttgtcccaa
      241 gcgtacgcag tttttggctg aacaagatca taagcttctt ctcttgtcat tcccttatcg
      301 atcaatttca gcataacgcg ctggctataa atcaaaccaa atgttgcaga catattccgc
      361 ttcatgtttt caggaaagac cgttagattt ttaacgatat tgccaaaacg atttaacatg
      421 taattcaata aaatcgtggt atcaggcaag atgattcgtt ctgctgaaga atgagaaata
      481 tctctttcat gccataatgt gacattttca taggcagtga ccatgtgtcc gcgcaccact
      541 ctggctagac cagccatatt ttctgaaccg atcggattcc ttttgtgcgg catagcagaa
      601 gaaccttttt gacctttcgc aaaaaattct tctacttcac gtgtttcgga tttttgcaat
      661 ccgcgtatct ctgttgcaaa acgttcaata cttgtagcaa taagcgccat cgctgagaaa
      721 tattcagcat gtagatcccg agggagaact tgagtagaaa tttcttgtgg acggatcccc
      781 aattgtttac atacatactc ttcaataaat ggcgggatat ttgcgaacgt tcccactgct
      841 cctgagattt ttcctgcttc tactcctttt gccgcatgtt caaatcgttc gatgttccgt
      901 ttcatttctg aataccatgt ggcaagtttc aatccaaaag tagttggttc agcatggaca
      961 ccgtgtgtcc gacccatcat cactgtatat ttgtgttctt ttgctttttc gccaatgatt
     1021 tccgtaaaac gtttcaagtc ttcccgtaaa atatcatttg cttgcttgat gagatagcca
     1081 taagcagtat ctacgacatc cgtacttgtt agaccataat ggacccattt tctttcttct
     1141 cccaatgttt cagaaaccgc tcgtgtaaac gcaacgacat catggcgtgt taatttttcg
     1201 atttccaaga tccgatcgat atcaaatgat gcatgctcac gaattttttt cacatcttct
     1261 tgcgggattt caccaagttc tgcccaagct tcatctgcta aaatctcgac ttcgagccac
     1321 gcacggtatt tgttctcttc cgtccagata ttccccattt cagggcgtgt ataacgttct
     1381 aacatgtttt ttctcctcta atcccaaata ttcgtttgtt tgatttcttc cagcgtttgc
     1441 tggatgtctt ctgtcaagat agtgatatgt cccatcttgc gacctttttt tgcttgtttt
     1501 tttccataga aatggaattg ccagtctggc ttattctcaa tcaagcgata gctttccagc
     1561 agttgttctc ccaaaacatt gaccattact gcattgctta tcaaacgtac tttttctcct
     1621 aatggccaac cacaaatacc tcgaatatgg gcatcgaact gactcataga acaagcttca
     1681 atagaataat gtccagaatt gtgaggacga ggagctaatt cattcacata tagcccgcct
     1741 gtttttgtta aaaacatttc tacccctaaa acacctgaaa ggctcactgc ttctgcaatc
     1801 acacgggcaa tacgctcagc ctcctcgatc acatcctgat caatcgcggc tggagcaatc
     1861 gtttcatgaa gaatattgtt gtggtggatg ttttccacga ccggaaaagt cgtgaaatct
     1921 ccttgaccat ttcctgccac catgatcgaa atctcttttt caaacgggat ccatgcttct
     1981 aaaacacagg ttccttctct taaaaggtcc atggcaggag ctagatccgc ccgacttttc
     2041 aaaacatact gccccttgcc atcatagccg cctcgagtgg ttttcaaaac acaaggatag
     2101 ccgatcccgt caatcgcatc ttggatatcc gtcggactga cgatcgttgc ataaggagca
     2161 atcacgatgt tattcgtttc caaaaaagat ttttctaaca ggcggtcttg cgtaatcgca
     2221 agcaagtctg tcccttgagg aataaatgac atgggtaaaa tcgcatttaa cgcctctaca
     2281 ctgacgtttt cgaattcata ggtaatgaca tctgttcttc tggcaagctc tttcagcgca
     2341 aaggtgtcat catagacact ttcaatatgc caatctgcaa cttgtgcggc tggacaatcg
     2401 ctcacaggat ccagcacacc tactttaaag cccatttctt ttgcactaat cgccatcata
     2461 cgacctaatt gaccgccacc gacgatcccg atcgttgaac caggcaacaa aggtttaacc
     2521 aagttgatca ctactttcta taacagtttt agccaacatt gaacgtcttt cagccaattt
     2581 agcctccaat tctaaatcat acatggaaag catctgtatc gcaagtagtc cagcattaat
     2641 tgctcccgct tttccgattg ctgtagtagc tacaggtact cctccaggca tttggacaat
     2701 cgaaagaagc gaatccaaac catttaatgt tcgtgattgg accggtacac cgataaccgg
     2761 caaagttgtt tttgatgcga ccatacccgg cagatgcgcg gctccccctg ctcctgcaat
     2821 aatgattttg attccttttt ttcgagcgtt ttgtgcatat tcaaacatca aatctggggt
     2881 tcgatgagca gaaacgacct ttttttcata agacacaccg aattcatcca atatttgaca
     2941 tgcttctttc attgtttccc aatctgaagt actccccata atcacagaga tttttgctgt
     3001 cattgttttt cctcccagtt tttctacatt catattttaa caatcacttt ttttgatgtc
     3061 aaagaaattt cgaatgttaa caaagaaaaa cattttattg ttcgtgttta ttaaagataa
     3121 aaaaaaggaa tgtgacaaaa gttaggtcac attccttttt atggataaac ggtgttctcc
     3181 tgccaactcc tcggtcacaa ggaacaatat tcaacaaaca tgagaagctt ttttttgaaa
     3241 ttgttccttg tgactcggag atgaacggca ggctcacgac ctcttccttc aacggtgttc
     3301 aatcagctga ccctcggtat tagctcaaac aaacggcaaa atacgaggaa ctatttttcc
     3361 gtttgtttct ctaacaatca ggtcaaaacg ctgatttcac aacctttctt ttgctctatt
     3421 acttcttgaa ggcttcttcc aatatttctt catctttttt caatccatta aagatcaaat
     3481 tcaatatgat agccgtaatg ctactcatta cgatgccatt tccagtgaac atttgcaatg
     3541 tttgcggcat ttgactgaat aatgtcggcg taacattgaa acccagccca aatccaacag
     3601 aaacagcaac aatcaataga tttttatcat tgctgaaatc aacttccaac agcatacgga
     3661 ttccttggac tgccaccatc ccgaacatga ctaacattcc tccaccaagt accggctcag
     3721 ggatgatttg tgctaacgca ccgattttag ggaataatcc taaaacaatc aagaagaatg
     3781 cagagaaata gatgggtttt cttgttttga ttcccgaaag ttgaaccaat ccgacatttt
     3841 gagaaaagcc agtataaggg aatgtattga aaataccgcc taagataacg gcaagtcctt
     3901 ctgcacgata tccctttttc aattcttctt ctccaacatt tttcccagta atgtcaccaa
     3961 gtgcaaaata tacacctgtt gattctacca tgctgaccat ggaaatgatg atcattagta
     4021 aaatcgacca tacgtcgaaa gttggtttac caaaataaaa gggttgtgga acatggaaca
     4081 acggcgcatc tccaatcgcg ctcagatcaa tcattcccat gaaagcagct aagatgcttc
     4141 ctccgactaa accaatcaat accgcgattg aacgaatgaa cccttttgcc agcatttgca
     4201 cgcctacaat taggagaatc gtcacaaatg caagtagcag atttgttttg tcaccaaaat
     4261 cagctgctgt tgcattacct ccacccattt tctcgattgc tacaggaatc aaagtcaaac
     4321 caataacggt gataaccgtt cctgttacta taggagggaa tagtttttta attttagaaa
     4381 agacaccaga aactaacaca acaaagatcc cagaagcgat aatagatccg tagatagctc
     4441 ctactccttc attggttccg atcaagatca gtggagctac tgcttggatc gcacatccta
     4501 aaacgacagg caagccaatt ccgaaaaatc ggttaactgt caattgcaat aatgtcgcta
     4561 atccacacat aaagatatca attgaaatca aataagtcat ctgttcttga ttaaagccta
     4621 gtcctgtacc gatcaaaaga ggcaccgcta ctgctcctgc atacatcgct agcagatgct
     4681 gcagccctaa aaccgcagct tttccatttt gtgtttcttt ttccacgatt atgcgtcctc
     4741 ctcaagaaac tcaacttgtc cattcgtcaa ggaagcaata cgcgcaagag aaacgactct
     4801 caatcccatt tcttcaagta actgacgtcc atcttggaaa gacttttcga tgacgatccc
     4861 gattccttct acttgcgctc cagcttgctg gcataattcg atcaatcctt ttgccgcttg
     4921 tccgtttgcc aagaaatcgt cgataatcaa tactttgtct tctgaagata aaaatttacg
     4981 agaaatagaa atcgtacttg tcacttgttt cgtaaaagaa taaacagaag ctgtcaataa
     5041 ttcttcatcc atcgtcaaac tttttgcttt tctagcgaag atcattggaa catctaattc
     5101 tcttgcagca aataaagctg gtgcgatccc agaagcttca atggtcacaa ctttcgtgat
     5161 tccttgttct ttaaatactt ctgcaaaccg ttttcccatt tgttccatca atacagggtc
     5221 tacttggtga gtgacgaagc tgtcgacttt taatacgccc tcgcctaata tacgtccgtc
     5281 tttttgaata cgttcaacta attctttcac tttatttgct cctttatcat ttattaatac
     5341 cgaaataacc gcttttgtgc aaaaacctac tatgtttctg ttaaagaaaa acaccctcca
     5401 ctttatcata agaaaagaag ggtgaaatag ccaatctttt acttatagtc cagcatttac
     5461 ggtactggct agaaactgtc atccatatta tgacgatata taagtaggta tgtaaaacta
     5521 ataatagtgt aaattcattt tcttgcaact gttttgcacg tgaaggcagt ttgccatatt
     5581 tgaataaaaa atgttgtttc aaatattgaa aacagtctaa cactactttc tgaaactgac
     5641 aacgtggttt acttattttt ttgtaaaatc taatgatttt taatgcatta ttcctttttg
     5701 ttcgggttta aatagttatt tttattttct cctatctaaa aaagcagccc acataaaaag
     5761 aaggcatgac aaaaagcatg tcatagcccc tttttccgaa taaatagtgt tcaaaagaca
     5821 gcctatcggc aataaggcaa gattttataa actacgggaa gtaatttccg taaatctatt
     5881 cttattcttt taagctgaca agctcgtcaa tcactactgc ttttcctgaa gcaatagaat
     5941 gatttgctgc gattcctgtc aaaatcgaca aaataccatc tacgtgagtt gctgcccgat
     6001 tccacggatc ttcttctttt tcttcaccaa atagatcatc taataaacgc ggatctcctc
     6061 cgccatgtcc gcccgcttct tgttcgacga ttacctcata tggctcaccg aacatcggat
     6121 agacatggat ggactgaaga acggctgctc cttcatcctt tttatctcca cctgcattga
     6181 tgtaagactt ttcgatcact ttcatttcga tccgtccttt gctgccattg aaagaaacat
     6241 tgaatccttc ccacggcata tacgcgttta gcgaatacgt caaaatcgca ccagtttcat
     6301 atttgaccgt tacgcctagt gtatcttcaa tagagatatc atcggaaaat acactgcggt
     6361 cacgaatata tccactgtct ttttctgctt ctaaatacat ttctcgcaat tcctcattgg
     6421 ctgacaggtc tatagcaaat ggatcagctt tagctagttc gcttccatag catcgactgt
     6481 aaaatatttc tactccgcgt ttctccgcat tatgcatccc ataaaagttt aatccaccga
     6541 atgcaaatac cttttgggga cggctgttca accaaaaatt cactagatca aaatgatggg
     6601 tagatttatg aacaagaagc ccgccgctat tgtgcttatt tcgatgccat cttctgaaat
     6661 aatctgcccc gtgttgcgta tttaacagcc attcgaagtg gatagaaaac acatcaccaa
     6721 tcacatcact ttctatcagt tcctttatct tcgtgtgata cggtgcataa cgataattaa
     6781 aagtgactcg caggcttctt cccgtctgtt tttggaccgt tattatttct tgagcttttt
     6841 cctcatcgat agtcagaggt ttttcgcaaa taacatcata tccttttttc attgcacgaa
     6901 tgatataatc atgatgcgtt cgatctactg acgtaataat gacttcagcc ggccgtacgt
     6961 catccagtag ttgctcaaat tgtgatggca tacatgtctt tacttttttc tgtccatact
     7021 tgttcacaag tatccgattt gcataatcca tccttgtttg agatacatca caaaaaccga
     7081 ctagttcaca agtttgatga tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc
     7141 caccaagtcc gacttgtaca tattttttct tcatttaaag tcattctcct tttcttttaa
     7201 gcaaaatact gtgacaaatg gcagccctgg atacagtaat gcagtgagta aaaaatcatg
     7261 ctgtatacac cagaacagtc catacaaaag gcataaatac cacagaaacc ccttgacgtt
     7321 ttttgacaac agccagatat tccttttgat caaataaaca agactatctg ctttatcttc
     7381 tttgcagata tcaattagcg tactaaaaaa actatgggat acaaaaagca acagacacaa
     7441 tagaaaagga atcagatacc aagctccaat ctttcccact aataaatacc aatcaactgc
     7501 aaaaataatg acaaacagct gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc
     7561 tttcctccaa catacctttt tttctgccaa gtaatggaca gatagataat aagaaggata
     7621 catcataaaa aataaatcag atagacaaca cgagcagaca gcactaatcc taaagtgaac
     7681 aaacatccta gaactaaagc gaaaagagca ttactgataa gactggttaa aaaaatccga
     7741 cagacatagt agttgagctt gtaaagaaac atctcttcta ttggcttttt catattcagc
     7801 ccttcagacc gcttgtactg atcccctgga caagatattt attgaagaat aagaaaatca
     7861 gaaaaaccgg tagcagcgac aacgtagaca tcgcaaacat actcgcgtaa gtcgtactag
     7921 atccagcgtc tgcaaataat ttgatggcaa tcgataccgt gtaagatctc ggcttgttta
     7981 aataaatcaa tggtcccatg aagtcgtccc atttccaata aaactggatg atgatcgtcg
     8041 tgatgatcga aggtttcagc aaaggaaaaa taattttggt aaagacacta tacttgctgc
     8101 atccgtcaat aatcgctgat tcatctaact ctttggggat gctgaccatg aattgcatga
     8161 tctggtaaat gaaaaatgct tgtccaaaaa aatgtggtaa aatcagcggc acatacgtcc
     8221 caaccaaatt cagccgattg taaataataa actgcggaat caaaatcact tgtgcaggaa
     8281 tcatcatcgt tactaacatg acgacgaacc agaatctttt tcctttgaaa tcgattctcg
     8341 ataatgcgta agcaatacaa gtcgagctca gaactgtccc tatcgtcgaa actgcagaga
     8401 tgacaaacga attcctaaag aaagtaaaaa atgaaattcc agcaaacccc gcccatcctt
     8461 ttgcgtaatt ttcccattga aaagtctctg gaatcaaatt cagcgaacca ctcataatct
     8521 cttgatccgt tttaaaagaa cctgcgatca tccataagac cggataaatc atgactagac
     8581 ctaatagcaa aataaaaccg tattgaaacc atttcgttac tgtattattc atttcttcct
     8641 cctctcattc acacaacgac ccattttttt gatgatttga aaatcagaaa ggtgacagct
     8701 cccataatca acagcatgat ccacgaaata gcactagcat accccataga attaaaagaa
     8761 aaagcttggt tatacacata aagagcataa aagttcgtgg catcattcgg accaccttgg
     8821 gtaatgataa atgcctgcgt aaaagtcata aacgccgcga ttgtctgcat aaccaagtta
     8881 tacaaaatga tcggcgaaag aagaggtaac gtgatcttgt aaaacgtctg ccatttgttc
     8941 gctccatcga tctctgccgc ttcataatac gagcctggga tctctttgat acctgcagca
     9001 aagatgatca ttgccgaacc gaattgccaa gcagacatca aaacaagagg gatcatgacc
     9061 agtttttgat cgctaaacca ggaaagcgca ggtgcgccta atcgttccag gaaaatattc
     9121 actaacccgt ttttagaaaa caactcccgc caaactagag cgatagaaat actgccaccg
     9181 atcaaactag gcacataata caaagaacga aagaaagtcg ttcctttgat attcctcgtt
     9241 aaaagataag caatcaatag accaaagatc agtttcacag gtacagatgc aaatacatag
     9301 agaaaagtga ctcggatgga gttcaaaaaa cgggcatcac tcagcatctt tatataatta
     9361 tccaagccga tccatgagac tggatttccc aaacgataat cggtgaacga ataataaagt
     9421 gacaagatca tcggtatcac tgttatcaat agaaatccta ggatgaatgg agaagcaaat
     9481 acgtaaccga ccacattgtc ttgatccaag aaccgtttta gtttttcttt tttcatgcct
     9541 tcctcctgct gctatcctag aatgtttgac gcaaactcat aaagactctc tgctgcttct
     9601 tctgctgtta cttcttcata aatgactttt tcaactaaaa gattgtattg gtcttgaatt
     9661 tccggattca aagcactatc aaagacattc acttcgccgt tgtccatatc ggaaatcatt
     9721 tctaaataat caaatgtcgc ttgcaaagtt gcatcatctg cacgagcgag ctgttctcga
     9781 atattcgaca tgattggtac accacgttct ccttttaaaa tttcattggc ttcttcactg
     9841 ttttgaaaga aatcaataaa cttagcagct tcttctttat gggctgagct ttttgccata
     9901 ctcagcattt gcgaagaacg taaagggata ccagattctc catctgcttt tctgcgaggt
     9961 ggattaatca actgaatctc catatcttca ggagcccctg acaatatttc tgtcaactgg
    10021 ttacttgata gaaacaccat tgccgcttct ctatccctaa ccgcctgaaa agacagatcc
    10081 gtcgtacttg cagctccacc acctggttct ggatgggccc ctttgtctac cagattttta
    10141 cgcagctgga aaaaatctgt tagatagtct ggattttcaa atcccaagct gttagataga
    10201 tcttcccgat tataaaaatt caatcctttc tcaacttgtg gtatttgaat aatacagcca
    10261 gctataaaat cttcgaatct tgaaatagca tagattccta attcttcagt gatttgatag
    10321 ctgatttgtt caaattcatc ccatgtccaa tttgaagctg gttcagggat atttaattgt
    10381 tcaaatatat ttttattgta agcaatcgca aacgaattca ccccgttaga cagtccaact
    10441 aattgcccgt cgtaagtcgt cgttgctaaa taggattcat ctgcattaga aatatcaatt
    10501 atcccttgct ccacatattc atctaatggt tcaatctgat tgatgtattg aggaaattga
    10561 ttgttcagtt ggaaaatgtc ccagatatca tctgatgctg ctagtgtacc taattttgtg
    10621 taatatcctt cataatcata aaattcataa tcgatcgtca catggggatt tttttcttca
    10681 taaagctcga ttacttttac tgttgcatcg tgtcggactt gcgaacccca ccaagccatg
    10741 ttcagcgtga tttttttgtc atcttcttgc ccacaagccg tcaagaagaa taacataaca
    10801 cttaaagcta agatgattct cattcttttc ttcattctct gcacctctac ttccattgtt
    10861 atttttcaat atactgtatt tttatacaga acatatttat taaacgatct ttttcaaggg
    10921 gaataaaaac caagacttgc cttatcgttc tgcctaggat gaacaactca atactttcaa
    10981 gaaaaattag atagtgctga ttcttctttc atcgctaaaa acacttcttg cttcaatgat
    11041 gggaggataa ttacgcaaag aaaaaaggat atcagacaac tgcccaatat ccttgacata
    11101 gctaaataag catgcttctt cgtgtgcgct cttccctaaa aaacagaaaa ataactagta
    11161 attctgtatg aaattaggta caatatacac gtcgatgcac aagcattgac tatggcaact
    11221 tgattggtca gttcccgctg acttttcaag ccccgacaat ttagctgcta ctaaattgtc
    11281 gggtgccttt tttagttttt attcgttttt cattctatag aaagcgcttc aataaatcaa
    11341 gacatttttt tgttagaatg ctaataataa caaaaggttt taactcttct tatgtacatt
    11401 acaggtaaaa aaacaaaaaa atttctcttt tttttgaatt ctgaacaaat agtactggag
    11461 tacaaaagac gaggaagagc cactatctgc tcttcctcgt cttttgattc tcacctgtta
    11521 ttcatgattc tttttctcag tttcccgata tttttccatt ttacgactaa aaagctctcc
    11581 gctacttggt ggcgtatatg tgatggcatt tgctcctgct tgaatggtct cagcaatact
    11641 ttcttccgtt ggaccacctg tcgcaatgat aggaagttca ggatattttt tacgaaaatg
    11701 gcgtactgct tctgcagttt ctttacctgc actgatattg ataatcgaaa taccagctgc
    11761 taacttttca tctatttctg tatatttcga cgttaccgta ctgataactg gaatatcaac
    11821 tactcgacaa acttctttta ctgtctctaa aggcgtagga ccgttcaaaa cgacccctaa
    11881 cgaaccttgc gcttctgcga acatgctcat gtaggaagaa cgagcacctt gtgtcaaacc
    11941 accacctaca ccggagaaaa ctggaatgtc agctgcctca ataatgcttt tagtaatcgc
    12001 tggatgaggt gtaaaaggat acacagcaat tactgcattc gcatttgtat tacgtatgat
    12061 cgcaatatcc gtagtaaaga tgatcgaacg gatcaatttt ccaaatatct tgatcccgct
    12121 tgcttctaaa atgatttccg gtactcttac gatatctttt ctcaattccg tcatgatttc
    12181 tggcgtaaat tgttctgcca tcagccactt ctcctttgct atctccggtc atactgattc
    12241 aattgatacg tctcgatgac ctggatgatt ttttgcgcat aaccggggtc tgtcgcataa
    12301 cctgcatctt gcaatgcttg tgcggcttcc tgatagttgg tggccgtaat cactttttca
    12361 tacagcgcag gattccaatc tactccttgg acgaacagtt gtgtatggtc atccatggac
    12421 tgttcccatg aatcatacac tttgaagtct ccttggatag ttatccattc ttcgttaaca
    12481 aattcctttg tttccaggct tactttcttc tgatccccat aagccttgat cccgaaaaga
    12541 ttattgtatt tgctagccaa ggtacttttt ccccaatttg attctaatat agcttgtcct
    12601 aaaatgatac taggaagcac tccataacca tcttgtagtt cttgggcatg cggaacaagc
    12661 cgttcaataa attgctgatg ggattgcgct tcattttctt gctgattatc agtaaaagga
    12721 gaagacagat tcttcaagga aaaaacaaag gctaagagta tcactgctac accggcaacc
    12781 actgctggcc aacggatatt cttgcgcttt tgctttttcc actttttttt cgccatgatc
    12841 aattcctact tttcatttaa ttcattcaga tattcttcta gcgtcaattg gtgtttggtg
    12901 atatactcag cgttttcttt accgacataa cgtaagtgcc aaggttcata tgtgatctta
    12961 gttatatctt cgtgtccttt tggataacga acgataaatc catactgcgc agcattatct
    13021 gcgatccatt tagctccagg ctgatctccg taacttgcat ccaatacagt agaaggataa
    13081 ctgttgtacc agttttgatc gacgacatcg actgccagac ctgtgtggtg ttcgctgtat
    13141 cctggctccg taatcgtttc tttagtaatc tttgtcgcct cctcttctga tacaccttga
    13201 cgactcatca catcttggac attttgttcg aaaacttgtt gttgggatga tacagaccgg
    13261 taagctgaga ccatcactaa tggaaatcca gctgcttcag cagccttggc aaatgcttca
    13321 tattctggag caatgcgttt gtcgatcaag tatccgttac ttaatgatgc caattgagta
    13381 gactcatcaa tttctttttc taatttgtga tctggaccaa ccaaaactag cttccaatcg
    13441 tctgttttaa catctggcaa ttgtttggac gtacgatctt ttgtttcttt gtcctttacc
    13501 ttgctgctgg ttgtttgact gttcttttct ttactagcag ttgttttggt gcttgttcgt
    13561 tcgactgctt gattgccggc ttgaacatgc gaatcatcat cgctaaaaat aacatagccc
    13621 attgctgcga tcataatcac aactgctcca aatcctaaaa atttattcac ttgaaaaatc
    13681 ctttcgttgt tcttcacgtt ccccattcgc ctcatgaacg taagtttctt ccagattttc
    13741 attcacccaa tcgagaagtt cgattttttc tccttcgatc tgtttttgaa catctgctgg
    13801 cagacgaaaa ttcaaaatat catcatagcc ccattcatca tattgaatac ttactttcaa
    13861 gtcaaggtat ctaccagcct gctgattatt tttcaaaggg atcagctcga cttttgctgc
    13921 tttgctgcta agccattttt gcgcgatgcg tgttttgatt tgcttgtact ctgcaacggc
    13981 aacgtttcgt ttttcgggaa aaataatcgt ttgacgcaaa atcttttgcg ttagcatcca
    14041 ttcataatca gtgaaaagat tttttacttt ctgcatcgct tcagatggca gatttttctc
    14101 gccatctttc catttttgcc attgttctgg actgatcagc aagtattcat ctaaaaaatt
    14161 tttttcagtt tcaaactgtt ctaataatac actcataata agatcaatat atacttgatg
    14221 cacgtttatc ccctcctttt aactattcta cttaagtttt gatcaaaaag cacctcttaa
    14281 atcgctgctt tcattttttt taatgtttca agtcctctcc tctttattct tcgtgatttt
    14341 tgttataata atagacagac aagaaactca atccaactat gaaggagcgt tttcagatga
    14401 aaaaatatca atggggaatc gttggtcttg gtaccatcgc acatgagttt gccgaaagct
    14461 tcaatcaaga gacgagcgaa ctaacagcag ttgcttccag aacttcagaa aaagcggaaa
    14521 acttcgctca ccgttacaat attccaaaag cttacggaag ctatcaagaa atgttagacg
    14581 atgctgaaat cgacatcgtg tacattgctg taccaaatag acagcatatc gaccatatct
    14641 tggcagcatt ggaagcaggc aaacacgttc tatgtgaaaa agccatcacg atgaataaaa
    14701 aagaactggc tgacgctatg cgcttagcag aagaaaagaa tttgatctta gcagaagcga
    14761 tgacaatctt caacatgcca ctttatcagc agttacgctc aataatggat acaggaaaac
    14821 tgggggcttt aaaaatgatc caagcacctt tcggcagtta caaagaacca gaccctaaaa
    14881 accggttctt caatccagaa ttagctggag gagctctgtt ggatatcggt acgtatgctg
    14941 tatctttcgc tcgtttcttt ttgagctctc aacctgaagt agttgcctct acaatggttc
    15001 cttttgaaac aggcgtggac gaacaatccg tcactatctt gcgtaacaaa gaaaatgaac
    15061 ttgctgctgt cagcttgact tttcaggcaa aaatgccaaa agtcggtatc gttgcctttg
    15121 agaacggtta tgtgacgatt gctgattacc cgcgtgctga tcgtgcggag atcttattta
    15181 cagatggtac aaaggaattc atcgaaagcg ggaatacatc tgaagcaatg aattacgaaa
    15241 taaagaatat ggtacacatg attgaaggtg acttgcccaa tcgctctctc ttcttgacaa
    15301 aagatgtcat cgaaatcttg gatcaaatgc aagttctatg gaaaaatatg taattttaaa
    15361 aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
    15421 cctatttttc gtttggctgg acttattacc gcagaggaaa gcttttgaac accgtttaag
    15481 aaagaggttg tgagcctacc gttcagctcc gagtcacaag gagcaatttc aaaaaacagc
    15541 ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttgacaggag aacaccgttt
    15601 aaagaagagg ttgtgaaatc agctttttga cctgaatatt agctgaattc acaacctcta
    15661 tttttggatt ttttgttggg agaattctgc cttgatttca ataaaacttt taaaagactg
    15721 caaaaactgg aacaattctg ctcgatcctc gaattcaggg cgactagtcg gcagaggcat
    15781 ttgccgatac aatacataaa cttcttcaat acgtgttaaa atttgtctgc catcatttga
    15841 ctctgaaaat gtttctgcag tataaatcaa taaaccatag atatgttttc cataataagc
    15901 tggttgctgg attctctcta gattttccag catgtcttta atcacattgg cctgtgctct
    15961 tctcatagag aagtaagttt cgtagtatag cggctgtctg agccagtagt tttcttgatg
    16021 ttctctggca tcttcttggc tttcgcgaat gaatgtcagt aaatgttcgc atttgatctg
    16081 cacatcttct ttattttctg ataaaagaaa ttctgccatt tgtctcaaaa tatttcgaaa
    16141 ttctttttcg atctgtattt ggttttcctt cagctgcttc gtacgatctg gcatgtaaac
    16201 atttgcaagc aaagcaaata ctaatccaat cgccattaat cccgcttcat tgccgatcag
    16261 ctgccaagac atgttttcct ccactaaata atgagtgacc aaaaccgaat ttactactat
    16321 cccttcggtt aatttgaagc gagaggcaag cgggatgaac aggaataaaa aaagactgaa
    16381 gccaaaaata gtatacccaa cagcaaaaaa actgaacagt gctgttgccg tagctaatat
    16441 aaaggctgcg attcgattca tgcccgtacg caatgtagag cgcttggtat ttcctacact
    16501 taataatgca atgatccctg cagctggcca atacaacagc gataagctac ccgctgtgac
    16561 catcgaaaag aagacactga tcactgtttt gactgttcgc attcctatac gcatttactt
    16621 cccccttttt tattttaatt atacttgaat aatgaaaaac tagctatcag acaaaagttt
    16681 aggtatgata gtaaaaagac caaaaaggag aatctctatg caatttggcc gttttcgact
    16741 aggtatgcgt acgatcaaaa gtgccttagc tgttttttta tgtatcttgt tttttcattt
    16801 gactcaacga ggaatcccga tgattgctgc attatctgct gttttttctt tgcgtcagga
    16861 cttgactaca accgtttctt ttggaaagtc taggatcatc ggcaatacat taggtggtac
    16921 attagctatc atttattttt ttgtcaaaag ctattttcac aatgattttt tggtagaatt
    16981 gatcttgctt cctattctag ttattattgt gatcgtcgtg tcagatggta ttaacaataa
    17041 ttccggtatc atttccgcaa tagctacgtt gttgctgatt gcgttgagca tccctcaagg
    17101 cgaatccgct ttgtatgcta tccaacgtgt actagatact tttataggga cattcattgc
    17161 aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa aagaaggaag aactatcaga
    17221 agatttggtc gaactccaaa agaaagaacg attgctaaga acaaatttac aagagataga
    17281 agctaagatc caaaaacaaa agaaaaatag cgaggactaa ttttcttttg caataaaata
    17341 aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt tttgattgaa cggtgaagtg
    17401 tactacttta ttcttccttc atcgagtttt gttctgcctc ttttggcgct tcatacatat
    17461 ttacttggct agtagtccca cgtgtcgaca aattctctac gatttccttc aagtcaagtc
    17521 ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa atttgtggca taatttgtca
    17581 ctcggttcgc tccactggtt tctcctgaac tgcttcctgt atcgacaaca gagatttttt
    17641 cgatatttcc tagcggctgt gctgcttcgc gcatcaattg cggtagcata tcgataacca
    17701 tactcaagac agctgcttcg ccatattcct tgaaggcatt ggcgatcttt tgttttgctt
    17761 cggcttctgc ttttcctttt gctagtgctg cttcggcttc tgcttgtcca gctaaacgtg
    17821 tcttattggc ttccgcttct gccagtgctt ccacacggaa tcgttctgct tcggcttcag
    17881 tcacttcttt gactttttga gcttgtgctt cttgttcttt agcgtaccga tcggcatctg
    17941 cttttttctt cacttccgaa tcatattgct tttcacgacg tgtgatttct ttttcttcca
    18001 gttcgatttg tttttgacgt tctacgattt taatctgcat ttcctgttcg attacttgtt
    18061 gttgtgctcg ggcactttct aaattgtagg cttggtctgc ttttgcttta gcaacatctt
    18121 gttcttgttt gtaggttgct aatttcagtt ctttttcttt taatgattct gcgatctctg
    18181 tttgtcgctg taattcagct gcttgtgatt ctttttctgc ttgagctttt ttgatacgcg
    18241 tttcttttag agcttccgct tctgctatat ctgcatctct tttgacttgg gcgattcttg
    18301 gtttccctaa agagtcaaga taaccgtttt tgtctctgac ctctttgata gtgaaagaaa
    18361 cgatgatcaa ccccattttt gcaaggtcta cagaagcgac ttcttgtacg ctttgactga
    18421 acttatcgcg attttgatag atttcttcta cagtcataga accaagaatc gagcgcaagt
    18481 gtccttctaa tacttctctt gcttcatttt ccagttcttc tcttgtcttg cctaaaaact
    18541 gttctgctgc tgtagcaatc tcttctacag atgaaccgat tttgatgatc gaagtaccat
    18601 cacacatcac cggtacgcct tgttccgtat acacttcagg ggtggagacg tccaatttac
    18661 tcgataatag actgatccga ttcgaacgtt gaaaaactgg caagacgaat gcgccacctc
    18721 cacgcacaat tttgattttg ttattgcttt catcaatgtg cacatttttg cttcctaaat
    18781 aacttccact gatgatcaac gcttcatccg gttttgctgt ctggtacttc gctacaaaga
    18841 cgatcagcaa catcagtaaa atcaaagctg cgattgcgat cgcgataaaa acaactggta
    18901 tttccattta acacacgctc ctttaagctt ttatcatgct ttcataaggc atgacataac
    18961 agattctttc cttgatctca atgatcaata ctttcgttcc ccgctcagct gtctgttctt
    19021 gggggctgta aaaacaagct ggacgactaa tggaaccggt aacacttttc aactgaattt
    19081 ctcccatacc tcgtacaggg atcggtgtga tcacaacagc tacttgtcct tccagagttt
    19141 tttcagaaga cgacatcgtt gattcggcat gtttcatcgg catcaatata tagaaattaa
    19201 gcaaaaatac caacactatc gatatgcctc cactagcaat aaataaaaat aaagatgatt
    19261 gatctgttaa acgctcacct agaaaaccaa ataagcttgt gaaagttaac caagggataa
    19321 taagcatcgg atcaagcggg ccatcaaaat cgaatacatc tccaaaaata atcaatagga
    19381 ctgccaatcc tgcacaaatc agcaatgtat accaataaat cgtctcaagt gcgattcccc
    19441 ctatcatata cacgcctcct tccatttcta cgtctatcat accaatgttt gaaaattctg
    19501 tatagcttct tttaccgact cagtggtgtt ttttatagga aggttgtgtt tatcgttgac
    19561 aatttgttaa atttttgttt tcatgttaaa attttcacaa taaattcttt cgaggtagcc
    19621 tatgtttcct ttttttaaga aaaaaccatt aaagctccag aacacgatca tccttgtcgt
    19681 ttgtttctcc acattcgttt ctttgattgt ttctgccata ttgatcagga attttattct
    19741 ttcgatgaac cgatctagtg tgtcagataa aatctctagc attgctaaaa tcgttgccaa
    19801 tgataaactc atccaaaaaa agctgcagac tggtgacccg acacatgcga ttcaaggaat
    19861 aacaaatgaa atcatgattg catcgaattt agattttatc gttgtcatgg accctgaagg
    19921 cattcgatat tctcacgcag acccaagtta catcggtctt cctttctcaa gtcatgaaga
    19981 tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa aaagaaggaa tgttagggtt
    20041 cggcttccgc tatttcgtcc ctatttatta tgatcaagag attattggtg cagtttgtgt
    20101 tggcttaaca aacaacacga ttgacaatca agtcaaagat ggacaaaaaa tgattgtttt
    20161 ttcattgatt attggactta gcgtaggcat cataggcgcc gtttttcttg cccaaaaaat
    20221 gaaaaaagta ctgcttggac ttgaaccaca agaaatagca gaacagttaa gtgaaaaaac
    20281 gattatcgaa aatgaagtag atgaaggaat cttagccatc tcttctgata aaactatcct
    20341 cttgatcaat acacctgcaa aaaacatttt ctaccaaaca gagcagctat tggatttcga
    20401 agtaggaaaa aaatcagtga taatctatat gacgttttgt ttaaagagac gattgagcat
    20461 caaacgaccg ttcggactca atccatgtat ttaaatggga tagaaactat ttctagcgtt
    20521 acacctcttt ttagcaatga ccgtttttta ggagcagtcg caactttacg aaatcgatca
    20581 gaagtaatga aattaatgga ggagcttggg gggacgaaac aatatataaa ctccatgaga
    20641 gctcaaaccc atgaattcat gaataaactt catgtcattt ctggtttgat tgatttgaag
    20701 aagtatgaag aagtcaccca ttttatccag cagctaaatc aaaattataa aaaagagatt
    20761 ggccaagtct caagtctgat caaagtacct gcaattgccg gttttttatt aggcaaaata
    20821 aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt tatccctaaa
    20881 atggagatgg atgactccat ccattcactt ttgcaagtgt tagggaactt acttgataat
    20941 gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac aggtccggat tcatttaaac
    21001 tatgacacag aagctgaagt attcatcgtg accgtctgcg atgacggtgt cgggatatca
    21061 gaagaaatct cagaaaaaat atttacgcta ggtttttcga cgaagggaat caatcgtggg
    21121 tttggattga atctcatcca ttcaatcgtc aaacgccacg gtggattttt agaattttcc
    21181 aaacaaacaa atggcggtac aatcgtctat ctagaattac caaaaaagga agatcaaaat
    21241 gatgacaaat gtcctgataa tcgaagatga tccaatggtt gcagaattga atcgtacttt
    21301 tgtggaaagc atccctaata tgaacgtaat cgaaagtttc agaaatgtag aagaaggaaa
    21361 aaaatatttc agacaaaaaa acgcagatct gctgctgctt gatgtataca tgcctggcga
    21421 aagtggtatc cattttttat cttggcttag acagcaagga aatgatatcc cagtgatttt
    21481 gatcactgct gcagatgata tcgaaaccat aaagaatgtg atccgctacg gtgccttaga
    21541 ttatttgatc aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa agtatcttgc
    21601 tttctctcag acaatccaaa cgactgaaaa agccgatcag aaattgatcg atcaagtatt
    21661 caaccacacc actcccattc acgaaatacc atcattcatc gatttgccta aaggtctgtc
    21721 aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta ttttttcaac
    21781 aaaagaactg gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat atttgaattt
    21841 tttagtagaa caagatatat tgaacgaaag aatcaaatat ttggaaatcg gccggccgct
    21901 aacctgttat gaattgacaa agaatgggcg gaaaaagctc acagattact gttgagtcat
    21961 cgaattatcc catacaaaat agtccaagta tacagaagta acaactgttt acttggacta
    22021 tttttaaaca ttttattgac gaatatccat gttttcttat cttaattgac gggctttagg
    22081 aagagatacc tcatttttcg tttttccagt ttctattatt tcacgaaagt tctcgaaact
    22141 tgtcgaaatc atatttgata atgcctcatc tgtatttgaa cctacatgcg gggttacaag
    22201 aactctaggg tacatttcta ctaattgctg gatagcaggg tctggaatcg tctcccatgg
    22261 cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg caaaaccagc
    22321 taaatgattg cttttcaaag ctcttaaaat agcttcatta tcttgaagct ctccacgggc
    22381 tgtattaatc aagatgctac cttttttcat ttgagctaaa aatccttcat tgatcatttg
    22441 atcgttttca cctgggatat gaggtacatg aatactgaca atatcgcttt tggctaataa
    22501 ctcttcaagc gagtggaacg tcaatacttt tttagcagct tctgtctgat gaatatcgta
    22561 gccgatcact ttggctccta gtcccttaaa caaaacagct tccgtcaacc ctatcctgcc
    22621 tgttcctatg atccctactg tgcaatttct gatttcacga ctaaacattt gctcatccac
    22681 tcgaaaatct ttgaaagatg ttcggatcgt cgtatacgca gtatttctca aaagcatcat
    22741 ggcaaaagtc aaagataact ctgctatcgc atttggcgaa tacgatggta cacgggctac
    22801 tgtcatacca aaatcagctg ccgcttgtag atcgatatga ttgactccca cagtccgtgt
    22861 aaaaacataa cgaatcccat attcctgcat ttttgcaaga ttggtctcat ctgctataca
    22921 atttcccctc aacaatacag catcatggtc ctttgctgac acgatattgt catgtgtcaa
    22981 cagttcttca taaagactga gatcataatg gtaaatattc aatttttcaa aaaaatctat
    23041 ctcgttcgga cgaacaccgt aacatgcgat tctcatactt tcttcctcca tttcttatga
    23101 aaataaacct atgccatgaa tcatttgtaa tacaacgatc catacaggtg tcagtaccac
    23161 agcaaaaata gttgacagta aggatgcatt ggaggctaaa agtgcctccc tatcaaaact
    23221 gatagcatag gctgctgcca ctgttgcagc tggtgtagcc atcatgataa cgattgttgc
    23281 cagtgcttca aaattgactg gtagaatatt cgttgccgtc aaaactgcta gaatcacgat
    23341 attcaatgca ggtacaaata ccactttgac tagactgtaa taccatgaag ttttttcaga
    23401 tgcagctttt ttgaagctta cttcacctaa tgttgcgccg attgctagcc acgccaatgg
    23461 tgaacttaaa ccagctaaat aatccatcgg ctgaaatagc cacggtgcag tctggtcaat
    23521 tcgcaaaaac gctacttggc ttatctctcc ttctgcacct gtaactgaca cttgtggcag
    23581 atacccttgg aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga ttggattgac
    23641 gaacatcgct ttgatatttt tagcttccat cttcaagcca ctcattttta tgtatccata
    23701 agaatacaaa aagattcggt agccaatatt gaaaattgag gaatacatca cgccgacagg
    23761 accataaatc gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa agatcgtcag
    23821 gacacgcaac gttgtttgct tatccccttt atagcttaag aaaaaaggtt ttgaaactag
    23881 aataagaata atataaataa ctaatcccca aatcaagaca ttgattcctt gcatcagtaa
    23941 ttctgtattg atatcttgca tgaaagctcg aaatgccagt gctggcaaag ccactgttaa
    24001 gacgactttc gatagaattt ttccaacaga ttcagaaaaa atcccctttt ttctacaaac
    24061 aaaccctaat aatataataa aaactgttga agtgattgca ctgacaatcg acatatctgt
    24121 caaagtcgat cgaaaaatgt caaataagtt catctttctc ctcctaatta tgttcattta
    24181 ttcacaataa aaaaagcttt tattttgcta atacgtatta acatttttaa ttttatcttt
    24241 acattgttac ttattgaaca ataggaaatt aaagaaactt ttgaaactta tgtaaagtga
    24301 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
    24361 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
    24421 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
    24481 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
    24541 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
    24601 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
    24661 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
    24721 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
    24781 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
    24841 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
    24901 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
    24961 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
    25021 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
    25081 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
    25141 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
    25201 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
    25261 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
    25321 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
    25381 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
    25441 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
    25501 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
    25561 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
    25621 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
    25681 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
    25741 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
    25801 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
    25861 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
    25921 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
    25981 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
    26041 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
    26101 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
    26161 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
    26221 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
    26281 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
    26341 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
    26401 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
    26461 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
    26521 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
    26581 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
    26641 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
    26701 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
    26761 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
    26821 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
    26881 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
    26941 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
    27001 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
    27061 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
    27121 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
    27181 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
    27241 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
    27301 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
    27361 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
    27421 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
    27481 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
    27541 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
    27601 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
    27661 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
    27721 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
    27781 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
    27841 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
    27901 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
    27961 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
    28021 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
    28081 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
    28141 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
    28201 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
    28261 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
    28321 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
    28381 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
    28441 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
    28501 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
    28561 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
    28621 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
    28681 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
    28741 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
    28801 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
    28861 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
    28921 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
    28981 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
    29041 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
    29101 ataaaaataa gatattcatg tctaaaaaaa
//
LOCUS       DACCWV010000019        27748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCWV010000019 DACCWV010000000
VERSION     DACCWV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.019"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            318..2084
                     /locus_tag="IVW84_000766"
     CDS             318..2084
                     /locus_tag="IVW84_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7916982.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2186..3445
                     /locus_tag="IVW84_000767"
     CDS             2186..3445
                     /locus_tag="IVW84_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7916983.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3592..4893
                     /locus_tag="IVW84_000768"
     CDS             3592..4893
                     /locus_tag="IVW84_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916984.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4896..5747
                     /locus_tag="IVW84_000769"
     CDS             4896..5747
                     /locus_tag="IVW84_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7916985.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5760..6575
                     /locus_tag="IVW84_000770"
     CDS             5760..6575
                     /locus_tag="IVW84_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7916986.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6726..7892
                     /locus_tag="IVW84_000771"
     CDS             6726..7892
                     /locus_tag="IVW84_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7916987.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8122..8769
                     /locus_tag="IVW84_000772"
     CDS             8122..8769
                     /locus_tag="IVW84_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7916988.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8853..9797
                     /locus_tag="IVW84_000773"
     CDS             8853..9797
                     /locus_tag="IVW84_000773"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7916989.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9932..11383
                     /locus_tag="IVW84_000774"
     CDS             9932..11383
                     /locus_tag="IVW84_000774"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7916990.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11404..12240
                     /locus_tag="IVW84_000775"
     CDS             11404..12240
                     /locus_tag="IVW84_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7916991.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12240..12899
                     /locus_tag="IVW84_000776"
     CDS             12240..12899
                     /locus_tag="IVW84_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7916992.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            13025..13714
                     /locus_tag="IVW84_000777"
     CDS             13025..13714
                     /locus_tag="IVW84_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916993.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13720..14676
                     /locus_tag="IVW84_000778"
     CDS             13720..14676
                     /locus_tag="IVW84_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7916994.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14720..15232
                     /locus_tag="IVW84_000779"
     CDS             14720..15232
                     /locus_tag="IVW84_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7916995.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15488..16036)
                     /locus_tag="IVW84_000780"
     CDS             complement(15488..16036)
                     /locus_tag="IVW84_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7916996.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16125..17306)
                     /locus_tag="IVW84_000781"
     CDS             complement(16125..17306)
                     /locus_tag="IVW84_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7916997.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17542..19050)
                     /locus_tag="IVW84_000782"
     CDS             complement(17542..19050)
                     /locus_tag="IVW84_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7916998.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19209..19400
                     /locus_tag="IVW84_000783"
     CDS             19209..19400
                     /locus_tag="IVW84_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7916999.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19603..20520
                     /locus_tag="IVW84_000784"
     CDS             19603..20520
                     /locus_tag="IVW84_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7917000.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20513..21586
                     /locus_tag="IVW84_000785"
     CDS             20513..21586
                     /locus_tag="IVW84_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917001.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21734..22252
                     /locus_tag="IVW84_000786"
     CDS             21734..22252
                     /locus_tag="IVW84_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917002.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22233..22625
                     /locus_tag="IVW84_000787"
     CDS             22233..22625
                     /locus_tag="IVW84_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7917003.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22701..23636
                     /locus_tag="IVW84_000788"
     CDS             22701..23636
                     /locus_tag="IVW84_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7917004.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23769..24548
                     /locus_tag="IVW84_000789"
     CDS             23769..24548
                     /locus_tag="IVW84_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7917005.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24541..24858
                     /locus_tag="IVW84_000790"
     CDS             24541..24858
                     /locus_tag="IVW84_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7917006.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25102..26712
                     /locus_tag="IVW84_000791"
     CDS             25102..26712
                     /locus_tag="IVW84_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7917007.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26869..27717
                     /locus_tag="IVW84_000792"
     CDS             26869..27717
                     /locus_tag="IVW84_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7917008.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8996 a         4902 c         5558 g         8292 t
ORIGIN      
        1 taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct agaatatact
       61 tctacgccaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
      121 agataaattc aggacactaa atagtacagg aagtagacta aaacttttaa aagttttaag
      181 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
      241 catttttgaa agcgttgtct tttgcttctc tatcacttat agtgatgacg tcatattaaa
      301 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
      361 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
      421 aaaaagtata tgtgctatgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
      481 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
      541 tgacgagcga aacaagtcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
      601 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
      661 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
      721 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
      781 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gactttttcg
      841 ggggagattt gcaaggtgtg atggatcatt tggattactt gacggacctt ggcatcaatg
      901 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
      961 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
     1021 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
     1081 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
     1141 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
     1201 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
     1261 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
     1321 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
     1381 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
     1441 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattt acagattcga
     1501 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
     1561 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
     1621 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
     1681 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
     1741 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
     1801 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
     1861 ttatttctga aggttctact cagtggctaa tagtaaatga tcgtgaagat aagctctatt
     1921 tcacacggaa actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
     1981 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
     2041 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
     2101 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
     2161 gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
     2221 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
     2281 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
     2341 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
     2401 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
     2461 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
     2521 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
     2581 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
     2641 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
     2701 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
     2761 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
     2821 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
     2881 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
     2941 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
     3001 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
     3061 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
     3121 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
     3181 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
     3241 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
     3301 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
     3361 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
     3421 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
     3481 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
     3541 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
     3601 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
     3661 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
     3721 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
     3781 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
     3841 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
     3901 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
     3961 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
     4021 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
     4081 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
     4141 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
     4201 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
     4261 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
     4321 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
     4381 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
     4441 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
     4501 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
     4561 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
     4621 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
     4681 atgatctatc tgtttaacaa tggcggtccc ggaagcgtag gcggaggagc tggttctact
     4741 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
     4801 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
     4861 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
     4921 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
     4981 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
     5041 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
     5101 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
     5161 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
     5221 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
     5281 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
     5341 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
     5401 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
     5461 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
     5521 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
     5581 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
     5641 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
     5701 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
     5761 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
     5821 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
     5881 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
     5941 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
     6001 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
     6061 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
     6121 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
     6181 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
     6241 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
     6301 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
     6361 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
     6421 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
     6481 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
     6541 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
     6601 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
     6661 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
     6721 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
     6781 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
     6841 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcttctt
     6901 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
     6961 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
     7021 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
     7081 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
     7141 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
     7201 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
     7261 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
     7321 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
     7381 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
     7441 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
     7501 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
     7561 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
     7621 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
     7681 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
     7741 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
     7801 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
     7861 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
     7921 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
     7981 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
     8041 taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt
     8101 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
     8161 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
     8221 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
     8281 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
     8341 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
     8401 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
     8461 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
     8521 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
     8581 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
     8641 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
     8701 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
     8761 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
     8821 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
     8881 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
     8941 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
     9001 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
     9061 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     9121 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     9181 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     9241 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     9301 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     9361 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     9421 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     9481 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     9541 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     9601 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     9661 tacagattgg taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     9721 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     9781 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     9841 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     9901 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     9961 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
    10021 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
    10081 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
    10141 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
    10201 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
    10261 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
    10321 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
    10381 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
    10441 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
    10501 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
    10561 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
    10621 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
    10681 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
    10741 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
    10801 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
    10861 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
    10921 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
    10981 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
    11041 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
    11101 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
    11161 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
    11221 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
    11281 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
    11341 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
    11401 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
    11461 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
    11521 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
    11581 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
    11641 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
    11701 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
    11761 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
    11821 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
    11881 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
    11941 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
    12001 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
    12061 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
    12121 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
    12181 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
    12241 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
    12301 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt
    12361 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
    12421 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt
    12481 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
    12541 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
    12601 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
    12661 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
    12721 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
    12781 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
    12841 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa
    12901 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
    12961 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
    13021 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
    13081 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
    13141 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
    13201 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
    13261 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
    13321 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
    13381 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
    13441 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
    13501 ttttccaaca gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
    13561 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
    13621 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
    13681 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
    13741 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
    13801 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
    13861 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
    13921 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
    13981 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
    14041 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
    14101 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
    14161 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
    14221 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
    14281 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
    14341 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
    14401 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
    14461 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
    14521 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
    14581 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
    14641 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
    14701 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
    14761 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
    14821 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
    14881 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc
    14941 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
    15001 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
    15061 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
    15121 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
    15181 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
    15241 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
    15301 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
    15361 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
    15421 tcgagaggtc gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc
    15481 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
    15541 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
    15601 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
    15661 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
    15721 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
    15781 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
    15841 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
    15901 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
    15961 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
    16021 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
    16081 aaatatttcc aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca
    16141 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
    16201 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
    16261 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
    16321 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
    16381 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
    16441 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
    16501 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
    16561 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
    16621 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
    16681 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
    16741 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
    16801 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
    16861 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
    16921 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
    16981 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
    17041 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
    17101 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
    17161 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
    17221 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
    17281 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
    17341 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
    17401 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
    17461 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
    17521 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
    17581 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
    17641 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
    17701 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
    17761 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
    17821 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
    17881 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
    17941 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
    18001 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
    18061 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    18121 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    18181 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    18241 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    18301 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    18361 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    18421 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    18481 cagttggttc aaggaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    18541 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    18601 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    18661 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    18721 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    18781 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    18841 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    18901 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact agcttcccac caaattctaa
    18961 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    19021 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    19081 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    19141 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    19201 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    19261 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    19321 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    19381 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    19441 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    19501 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    19561 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    19621 aatgcaaatt atgagccaga cgaactagag gagacgaatg cggatcagga tacaggacaa
    19681 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    19741 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    19801 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    19861 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    19921 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    19981 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    20041 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    20101 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    20161 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    20221 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    20281 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    20341 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    20401 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    20461 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    20521 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    20581 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    20641 agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    20701 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    20761 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    20821 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    20881 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    20941 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    21001 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    21061 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    21121 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    21181 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    21241 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    21301 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    21361 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    21421 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    21481 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    21541 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    21601 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    21661 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    21721 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    21781 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    21841 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    21901 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    21961 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    22021 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    22081 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    22141 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    22201 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    22261 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    22321 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    22381 gtcaaaactt ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta ctgagtattt
    22441 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    22501 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    22561 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    22621 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    22681 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    22741 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    22801 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    22861 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    22921 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    22981 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    23041 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    23101 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    23161 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    23221 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    23281 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    23341 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    23401 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    23461 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    23521 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    23581 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    23641 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    23701 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    23761 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    23821 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    23881 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    23941 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    24001 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    24061 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    24121 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    24181 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    24241 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    24301 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    24361 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    24421 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    24481 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    24541 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    24601 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    24661 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    24721 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    24781 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    24841 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    24901 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    24961 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    25021 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    25081 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    25141 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    25201 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    25261 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    25321 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    25381 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    25441 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    25501 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    25561 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    25621 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    25681 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    25741 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    25801 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    25861 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    25921 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    25981 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    26041 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    26101 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    26161 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    26221 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    26281 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    26341 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    26401 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    26461 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    26521 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    26581 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    26641 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    26701 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    26761 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    26821 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    26881 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    26941 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    27001 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    27061 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    27121 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    27181 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    27241 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    27301 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
    27361 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    27421 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    27481 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    27541 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    27601 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    27661 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    27721 cttttattca aaaaacatac caaaaata
//
LOCUS       DACCWV010000020        27334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCWV010000020 DACCWV010000000
VERSION     DACCWV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.020"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            103..828
                     /locus_tag="IVW84_000793"
     CDS             103..828
                     /locus_tag="IVW84_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7917009.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDVLNFEQMFVEGNEPDSYGRNRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1005..1346
                     /locus_tag="IVW84_000794"
     CDS             1005..1346
                     /locus_tag="IVW84_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917010.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEQLACDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYERDNLIDISYYGQGKK
                     YIAEMEAELDE"
     gene            1339..2085
                     /locus_tag="IVW84_000795"
     CDS             1339..2085
                     /locus_tag="IVW84_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7917011.1"
                     /translation="MSEKEQPLKNRSDNTLFNSLYKINVKGVTEKRNDLTYLSWAWAW
                     AEVSKVCEAVDYEIYRDPETHRPYLFDEKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKKIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            2091..2777
                     /locus_tag="IVW84_000796"
     CDS             2091..2777
                     /locus_tag="IVW84_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917012.1"
                     /translation="MFKPLIDSYSAVLRRFKGNQIVARINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2780..3610
                     /locus_tag="IVW84_000797"
     CDS             2780..3610
                     /locus_tag="IVW84_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7917013.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDIDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            3627..4478
                     /locus_tag="IVW84_000798"
     CDS             3627..4478
                     /locus_tag="IVW84_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7917014.1"
                     /translation="MESLASTMEKLIRRVLVQSGRCPECSEPLYSWRGKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4475..4834
                     /locus_tag="IVW84_000799"
     CDS             4475..4834
                     /locus_tag="IVW84_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7917015.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            4849..5010
                     /locus_tag="IVW84_000800"
     CDS             4849..5010
                     /locus_tag="IVW84_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7917016.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5007..5312
                     /locus_tag="IVW84_000801"
     CDS             5007..5312
                     /locus_tag="IVW84_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917017.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            5312..5629
                     /locus_tag="IVW84_000802"
     CDS             5312..5629
                     /locus_tag="IVW84_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7917018.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            5626..6012
                     /locus_tag="IVW84_000803"
     CDS             5626..6012
                     /locus_tag="IVW84_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917019.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            6009..6344
                     /locus_tag="IVW84_000804"
     CDS             6009..6344
                     /locus_tag="IVW84_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917020.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            6490..7059
                     /locus_tag="IVW84_000805"
     CDS             6490..7059
                     /locus_tag="IVW84_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7917021.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            7226..7531
                     /locus_tag="IVW84_000806"
     CDS             7226..7531
                     /locus_tag="IVW84_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917022.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            7555..7752
                     /locus_tag="IVW84_000807"
     CDS             7555..7752
                     /locus_tag="IVW84_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7917023.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            7749..7955
                     /locus_tag="IVW84_000808"
     CDS             7749..7955
                     /locus_tag="IVW84_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917024.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            7955..8251
                     /locus_tag="IVW84_000809"
     CDS             7955..8251
                     /locus_tag="IVW84_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917025.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            8327..8740
                     /locus_tag="IVW84_000810"
     CDS             8327..8740
                     /locus_tag="IVW84_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7917026.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9617..9961
                     /locus_tag="IVW84_000811"
     CDS             9617..9961
                     /locus_tag="IVW84_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7917027.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9966..10247
                     /locus_tag="IVW84_000812"
     CDS             9966..10247
                     /locus_tag="IVW84_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917028.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10350..10664
                     /locus_tag="IVW84_000813"
     CDS             10350..10664
                     /locus_tag="IVW84_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7917029.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10642..12336
                     /locus_tag="IVW84_000814"
     CDS             10642..12336
                     /locus_tag="IVW84_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7917030.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12356..13534
                     /locus_tag="IVW84_000815"
     CDS             12356..13534
                     /locus_tag="IVW84_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7917031.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13497..14183
                     /locus_tag="IVW84_000816"
     CDS             13497..14183
                     /locus_tag="IVW84_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7917032.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14183..15343
                     /locus_tag="IVW84_000817"
     CDS             14183..15343
                     /locus_tag="IVW84_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7917033.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15353..16228
                     /locus_tag="IVW84_000818"
     CDS             15353..16228
                     /locus_tag="IVW84_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917034.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16225..16536
                     /locus_tag="IVW84_000819"
     CDS             16225..16536
                     /locus_tag="IVW84_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917035.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16526..16879
                     /locus_tag="IVW84_000820"
     CDS             16526..16879
                     /locus_tag="IVW84_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7917036.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16869..17270
                     /locus_tag="IVW84_000821"
     CDS             16869..17270
                     /locus_tag="IVW84_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917037.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17263..17667
                     /locus_tag="IVW84_000822"
     CDS             17263..17667
                     /locus_tag="IVW84_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917038.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17679..18284
                     /locus_tag="IVW84_000823"
     CDS             17679..18284
                     /locus_tag="IVW84_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7917039.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18304..18666
                     /locus_tag="IVW84_000824"
     CDS             18304..18666
                     /locus_tag="IVW84_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917040.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18669..18851
                     /locus_tag="IVW84_000825"
     CDS             18669..18851
                     /locus_tag="IVW84_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917041.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18868..22287
                     /locus_tag="IVW84_000826"
     CDS             18868..22287
                     /locus_tag="IVW84_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7917042.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            22338..23075
                     /locus_tag="IVW84_000827"
     CDS             22338..23075
                     /locus_tag="IVW84_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7917043.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            23085..25376
                     /locus_tag="IVW84_000828"
     CDS             23085..25376
                     /locus_tag="IVW84_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917044.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            25400..27088
                     /locus_tag="IVW84_000829"
                     /pseudo
     CDS             25400..27088
                     /locus_tag="IVW84_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9832 a         4083 c         5887 g         7532 t
ORIGIN      
        1 atatatatgg ttaaagtcgc agtaagaatg taattgagct ttttcctcat agaatcacct
       61 cactttctaa cgaaaattat accaaaaagg agagaagaaa taatgcaaga attagtaatt
      121 ttgaaaaata aagaagctgt aactacgagc ttacaagtgg cagaaagttt caaaaagaaa
      181 cataagcatg tactagaagc aattgaatca ataaaaagat cggtcgaaaa ttcggccaat
      241 gttgaagatg tgctcaattt tgagcagatg tttgtggaag gtaatgaacc agattcatac
      301 ggcagaaatc gacgagttta tttcatgaat agagacggat tttccttgct agctatggga
      361 ttcactggaa gtaaagcaat aaatttcaaa ctaaaattta ttgaagcttt caacgaaatg
      421 gaagatgtta ttcggaagaa tactgttcct caaacaattg aagacatgat gatctatcaa
      481 ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc ttaaagatac tatgcgaatt
      541 agcggacaac aagagtttga aattaagcaa aaaggaaata tgaaagttat ggaagttcta
      601 gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa aagtattctc aaaattttgg
      661 tctgaattta aacgtacctt ttcaatccca agatatggcg agttacctcg taagagattc
      721 gatgatgctg tttcatttat tgaaatgtgg ttaccagaaa ctgcgatccg catggaaatc
      781 gatcaactga acagacaaca aagacttttc ggtgatgaaa atgaatagag ctgaagcgct
      841 aagaataggg acggtaattg ctaatcgctg gtggagacac aataaaccaa gcatcctaag
      901 ccaacaacat attgataagc aaaaagcttg gcaacaaata aaaaagtgac tccgccggca
      961 agcatagagt cacaaagaaa atacatctaa ggagatgtta ccacatggaa aaagaacttt
     1021 ccactctaga tcaatatttg actgatccta gttggggcaa atcgaatatc aaggaaacaa
     1081 gcaatcgaaa aatcagacgt aatcttttga cggatgaaca actagcatgt gatcaagacg
     1141 atttgggcaa ttttgtgagt atttgggatc atgtctatct tatccatcta tcaaaacatt
     1201 caaacaaacc tgaatacatc tatgtcatcg aagatggctt gattgatgcg ctcgaagagt
     1261 acgaaaggga taacttgatt gatatctctt attacggaca aggcaaaaaa tacattgctg
     1321 aaatggaggc agaacttgat gagtgaaaag gaacaacctt tgaaaaatag aagtgataat
     1381 acgcttttta atagcttata caaaataaat gtgaaaggtg ttactgaaaa acgaaatgac
     1441 ctcacttatc tttcgtgggc atgggcttgg gcagaagtta gcaaagtgtg tgaggcagta
     1501 gattatgaaa tctatcgtga tccagaaacg catcgtccat acctctttga tgaaaaaaca
     1561 ggctatatgg tttttaccag tatcacagtc aacggagtaa agcgtgacat gtggttacca
     1621 gtcatggatg gtgcaaacaa ggcaatgaaa gatgagccat atacctacga agtcaatgat
     1681 tatcagtgga ataacgaaac gaagaaaaaa aagattgttg gaaaaatcga aaagcgagtt
     1741 gaagcagcaa ctatgtttga tatcaacaaa acgatcatgc gctgtcttgt aaaaaatcta
     1801 gcaatgtttg ggctagggct atatatattt gctggcgaag atatgccaga agatgtctcg
     1861 atgcttgaac cagctactca aagaagcaaa aagctattct tggatgcttt acaactggtt
     1921 gctaacaagt acgaaaaatc aattgatgaa gcaattgttg cattgactga tgcagcttcc
     1981 ataaccgctg atgacagtaa atggaccaag agagacttgg gcattctaaa acgaggcgtt
     2041 aattggcttg aagatcagta cagagaagaa acaaaagaga agtgatatga atgtttaaac
     2101 cgttaataga ctcatactcg gcggtactaa gaagattcaa aggcaaccag atagtggcaa
     2161 gaatcaatga agaagtgaat atcgagcgat tgaagacgat gtatgacggc tacgatggcg
     2221 atcgagtcat tgaaattcgc tttattgatc cacgtcggtt cacagtacag caaagaaact
     2281 tcatctatgc gctcataggc gatattttca tcgatacagg catgccaacg gacttctgga
     2341 aggaattctt ctacttccgt tttgaaggtg tcacagggcg caaaataagc ctcaaagatg
     2401 aatcgaatac aactgtgagt gatgctaatg tcttagcaaa tatcatttta gatttcatct
     2461 ttgaacatca tattcctttc aaagaaggat atgagatttt accagcgaat caagagtatt
     2521 acttctacaa atgcattaca aaaagagtct gctgcatctg tggcaaaaca ggagctgata
     2581 tcgatcactt tgacaaagcg ctgggaagac gaaagcgcaa ggaagttgat cattcagagt
     2641 acacatttgc agcactctgc agaatccatc acacagagaa acacaaaata ggtgtgatca
     2701 atttcaaaaa taaatatcaa atcaaaggaa tcaagttaaa ccaggaaaca attaaaaagt
     2761 taaggatagg aggataaatt tggctgagat aagttggatc aaacttaaaa ctactatgtt
     2821 tgacgatgaa aaaatacgat taatccaagc tgttcctgag tcggatgcca tcatcgttat
     2881 atggattcga ttactagttt tagcaggaaa gactaacgac gatggtctga tatatatcca
     2941 gaggaacatg ccttataccg aagaaatgct tgctacattg ttcggcaaaa acgtaaatac
     3001 ggttcgccta gcgctaacta cattggcaaa tttcaacatg attgatctag gcagtgatgg
     3061 actgattgcc atcagtaatt gggaaaaaca tcaaaatatc gaagggatgg ataaagtaag
     3121 gctaaaaaac gctgaaagaa accgtaaata cagagaaaga aagagacagg aacgtctcaa
     3181 attggaaaat gacgttagcg tgacgtcacg tgacggtaca gataaagata tagaagaaga
     3241 tatagatata gataaagaag aaaagaaagg taagtattct gacgaacact tacgccttgc
     3301 taaaaagttg caaagtaatt taactgaaga ttttccaaaa gaaatgaata aagtagatat
     3361 cgaaaaatgg gcagacacaa tcaggttgat ggaagaaaga gataaagcat ctatagaagc
     3421 gattgagtat gtgatcaatt ggctacctac aaatgaattt tggtttggaa atattagaag
     3481 tgctaagaaa ctgcgagaaa aatttgagaa gcttaaattc gaaatcaagg cagataaaaa
     3541 taatcataaa aagcaaagtc aaaaattaca gtacagcaat cctagtgaat atgacgactt
     3601 gccaatttaa aaaggagatg catcacatgg aaagcctagc aagtactatg gaaaagctaa
     3661 taagaagagt tttagtacaa agtggaagat gcccagagtg cagcgaacct ttgtatagtt
     3721 ggcgagggaa aaataaggac gggtcagaac gttgtaaacc aacatgcatg agttgtggtt
     3781 ataaagcgtt acgtgtgaaa gaggatatac agaccgaacg gatatataac gacagcttaa
     3841 aagcacgagc gttgagtttt tttcaaaatg gttcggtatt aacagataaa actttgttta
     3901 aatgcaaaat ggaaaattat cacgtagtgg atcaagaaac aaaaatcgct ttagaaaaag
     3961 caaaaagcta tacgaatgag gttctgctga accatcctgc acatttcatt ctatcaggga
     4021 aatcaggaag cgggaaaagc cacttatcaa tggctacagc ttgggaaata cttgagcgct
     4081 caaattatga caagaaaata ctttttataa gctatcaaga attattagag caaataaagt
     4141 tttcttataa caatgctgaa ctgagaaaag aaattgaagg atcgcttata gccgatatta
     4201 aaacaactga tttggtggtt tttgacgata ttggagctga attaggtagt ggggtatcaa
     4261 atagtaggca gtttacaaac aacacgttaa acacgctctt agaagcaaga cagaacaagg
     4321 caacgatcat cacaacaaac ttatctggtc ctgaactaag agaagcctac ggtgaaagaa
     4381 ttgtttctag gatatttaag aattcagaag gttatgcgct gaaattccag caaacagcag
     4441 acaagcgcat aaaaccagtg aaaggtagta tcgcatgaat aaataccgta ataaaaaaac
     4501 tgttcatcga ggtatcaagt ttgattctat cgcagaagca gagtattacg atctagcctt
     4561 gtggcaagct gaagcgaatg gctggaaagt aaaacttcag gaaagatttg agctgatgcc
     4621 gaaatttgaa ctagacggaa agaagtatcg caagatcgag tatattcccg acttcacatt
     4681 ttataaaaac ggcaaacttg tcaaagtcgt agatgttaaa ggaatgcaga caaaagactt
     4741 taagatcaag gcaaagctgt tttgtcatca atatcaagtg ccgttgatat tagctaaaaa
     4801 atatcggaat acgttcaagg aagagcgttt ttaaagaggt ggtctatcat gacaacagaa
     4861 gaagtgattc aaatgcgaat tcgaagcatt caacgtgaga ttgacgatct ggaacgaaca
     4921 aaggcagtga tggtcaatga aacggcgaga aaggcaatcg atttgcacat agagaactta
     4981 agaagggaaa ttcgtagatt ggaggaatga gcgtggataa gaaagcagca atgaaacgaa
     5041 tcatcgaact gacacattct gagaattggc aagaagacaa agaaatagtt gcagaagtcc
     5101 aaaagctcgg taaatcaatg tggactgaaa aaaccagacg gagaatgccg agaaaaattg
     5161 caatctggca cggtgaccga attctagtaa caggtaccgc tgaacagtta tctgaaatta
     5221 ctggtctgag caaaaacatc atctgggata gagcgaagaa tatggatatt gattctaaag
     5281 gaagacagtt taggtatgtg gaggagaaat aatgaatctc attacacaat acagtgatat
     5341 catcctcaag aaaatcatga tgaagattca gaaagacaaa aaatcaaaag aacgagcgga
     5401 attagttaag ttggaaatgg ctgaaacagg ggcaggagtg cgaagtagca ggcattggaa
     5461 ggcagcagca aacattgaat tttattacaa cgaaattcaa aaagggttcg atcaaatgcg
     5521 tgagctggat cggcaaacaa attggagcaa gaaacttcat caagatcgtt tcaaatttgt
     5581 agagaaatat aaagagatat tagacgaata catggagggc agcaaatgat accgagattt
     5641 cgatcgtggt acacaccatt taaaggtaaa acaattggac aagaaatgaa atatgggcaa
     5701 gcaggaaggt tgatcactca tgctgaaatg gctccagata aatatgtgct tatgcaatcc
     5761 acaggactga aagataagaa tggcgtagaa atatttgaag gggatgtagt tctggttaat
     5821 gtaagtaatg gttttgatca tttagttaat gaaaaaacgg ttgtacaaga atccgagttt
     5881 cattccggat tagtttgtaa atcattaatt agtggaatgg aatatagaat attcagccac
     5941 caaaagatgg gatacgaata tgaagtcacc ggaaatatac acgagaatag cgagttattg
     6001 gaggtagagt gattatctta gctactgact acatcgattc tttaaaagat gaaaatggga
     6061 atgtcccagc attgctaaat tgtggaatta aaagtaagaa tagaatcatt ttgttacgga
     6121 tatcggctga tttagcaaaa tatctttatc tgagcaagac agcagtatat tacactgcgg
     6181 ctacacgtta tcagtacaag ggaaaaacaa tcagtcagga ttattatgat cgtttaatca
     6241 gtcctgacat acatggaaaa tcaaaatcag cgatcaagtt atttggcgca atagagatat
     6301 atgccgatga ttttccaaat ttatggttga aggaggaaca gtaatgaaag actggttaga
     6361 agaagcaagc gccttagttg gctgtttcat aaactgtgcg tttatatctg taattattgc
     6421 actggtggga tttgtatcat tgaaagtgat tatattgctt tggcagttaa ttttttagga
     6481 ggaacagcga tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agtgatagga
     6541 aatgatgcac tagctgcttg ctataacgag agtataaatg caggtattac gttaatgaaa
     6601 aaaatagacg aaccgaagaa gctagttatt ccacaactcg tggccggttg gcttgagaaa
     6661 tctacggacc cttttacgaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt
     6721 gattcatatt atttctgtga ttggtttgta cgagatggca tagtgacgca agaacaagga
     6781 gaagaattac tcgcttgggc aacgagacaa tcatatgaaa cactattgag cttgtacaac
     6841 ggctacgagg tcgagaaaga gccgttgtac agtgtaataa ttgctggcga gtaccttgtc
     6901 aaggagatca gttgcagtaa tgaagttaaa ctagtatcaa cagcttacct gccacaatac
     6961 cttgcacacc actaccaact gaccgaaaaa cagatcaaag caattgatga aagatactgg
     7021 ccatttgctg tgccagtgga agaggtggcg gaaggatgag tagacagata atgctccata
     7081 taccagacgg aacaaaggca atgacgatta ccattgttgc tgcacaaaaa gacggtgggt
     7141 tggcgttgaa aacaaaaagc attgatacca aacagatttt agaaggcaga gatgtcgaga
     7201 tagagatcga tgaggaggaa gcggaatgaa tgttcaagtt tatttaaaaa gagatttggc
     7261 caggtttcct cgaagctggg atacaaattc ccacccatcc gaggcagaag aagagggcta
     7321 tctaatgtct gctaacatga tagaaattac aagagatgat gcggatgagt ttgataaaaa
     7381 aagatcatgg tactcggttc cgaatccaat gtatacggct gtaatagaag attatcatat
     7441 atccgacaga tttattctga ttgatctaga aaggcctaag aaacgaatta aacagtatag
     7501 acgttgtgga ttttcaacaa agaaatggtg ataacaaaac agaggtgaag aaaaatgaac
     7561 aacagacacc gcagagtagc aaaactaaga aatcaagaac tgaatgtagc gaaagtaaag
     7621 ttcgaacggg agtatggtgt ttctgcagaa gaaacatata aagtggcaag tcagtgtgtt
     7681 gctgatgcaa gtgatgctat tcgtaagttt ggaatttcga tactagatat taaatgggag
     7741 gatacagaat gaagatttat gtagtaaagt ttggaaatca attttacaga agcgatgaac
     7801 gttctatagg ggctaacaca ttatccattg tagacatact ccaaagcgca agatggtttg
     7861 ataatctcga agaagctaac caagtttcac gacgacttgg tggattaacg caagtatatg
     7921 aactggtcac tgtcgatcat gagggggtag agtaatgaaa ctaaaagacg gattttacgc
     7981 tagtagtcat ggtatcggcg gtttgatgct agatatgccg acaaagaacc ctaaaacacg
     8041 tgagaaacca aaattcaaag tcggtgacat ggttcgctgt gaagcagaag gatttatcta
     8101 tccatttcgt ggatatgtag aaaaggtatt gtcgaactca gcaatcattc gtattgaaaa
     8161 cacgatggaa tgtgacaagt ggttagcgaa aagcaaagag aatttagctg tagcgagatt
     8221 agttgacatg gaagtaatcg agagcaaata aaaaagccgg atcgctccga ctaacataat
     8281 aaaacagaca agtttattat atcacataaa ggagcggttt gacttgatgc aattgttacg
     8341 agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga actttcggcg
     8401 tttggagcgg atggcaggtc gctctttgat agatattaag tctccaatta ttacggatat
     8461 gccgaaggcg ccgaagcacg gcaataaggc agaggacgcg atcattcaga tgatggatat
     8521 agaagcagag agagatgcga ttttagcggc tttgatggct cttagtctga ttagccgtca
     8581 gatactctac tacagctttt gtgtgccaga tagcttctca aactacagaa ttagccgtga
     8641 agtgggttat tcagaaagaa gtatacaacg gatgaagtcg gaagctctaa tagagtttgc
     8701 agaagcatat aaacacggaa gaataattgc ttataaataa tttggcggtt ttttggcgga
     8761 atgatggcgg tttttagcta tttaccagtg atattatggt agtgtcgaaa gattagtgat
     8821 aggtctgaga caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc
     8881 ttcgatagac agcaacggaa atattaagaa taaggatgtg aatttcaact ccttctaaat
     8941 tgttcttatt atctatcatc cgttgctgtc tattgttttt ttaattattc acacgataac
     9001 taaaggtgag tgaagagaaa tgattccatt aataatttca atttttgcgc tctgtcttaa
     9061 tgtctatatg attggattta aaaatgggca aaataaaaaa tagtagcagc caagaataat
     9121 tttatagtgt cactgtggcg gaaagggtag acgcttaaaa ataaggtcaa tacgtcgagg
     9181 gatagcctta acgttttatg atttgaccat gcaatgttcg attcattgcc agtgactttg
     9241 gcaaccgagg gttggaaatg ggcgctcaaa gtacacgagc aaggcgaggt cgatagtaat
     9301 cgatggaatc ggtgtaggtt gcttgataga gctataactg catctcgttt atgaggtgta
     9361 gtttttacag attagatcac tctttgagtg gtctttttat tttacacaaa ggaggtaaca
     9421 agaatgtata gaccacaata cttagaacag aagtatgaag taatcactgt gcaaaacggt
     9481 aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata caaacgaaag
     9541 gaaagcaatg aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg
     9601 cgaaaggaga cggaatatga ccgaggaatt ctatagatgg ctattacagt tgataagaga
     9661 agatcgtttg gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat
     9721 gaaacgagat cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga
     9781 gaacgttcat catataaagg aagtcaagga tagacctgac ttagctttag atttagataa
     9841 tcttatttgt ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcgtt
     9901 agataaacaa gagaagaaga tagaaagctt cgctaacttc gatgcaagtg aaaggtggta
     9961 agtgcatgat catcaatgac aatggcagag agtatgatac agaaaagatt gaagagtatt
    10021 catcttatac tcagggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg
    10081 atctgttatc agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga
    10141 ataaagataa taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta
    10201 attattacat tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa
    10261 taaatcgcaa attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt
    10321 tcgaacttta tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct
    10381 acagagaaaa attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg
    10441 agccattctt agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg
    10501 aagaagatat atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag
    10561 cgttgcttgt ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc
    10621 ctgaattggt aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg
    10681 ttcaaagcta tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat
    10741 tatctagaag aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag
    10801 gattcaatca atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt
    10861 ctacaatgtt ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga
    10921 tatctttcat taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc
    10981 tttctactaa ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat
    11041 gaaagccaag caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat
    11101 ttagataaaa aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc
    11161 aaacttcgtt ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac
    11221 cgctttgatg aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt
    11281 ggtggtaaaa ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt
    11341 ggtccgcttg atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt
    11401 gacaaggatg gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat
    11461 aatgaagttg atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca
    11521 gatttaaaac ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga
    11581 cttacgttca tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct
    11641 tcatatgatg atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg
    11701 ataggaacag tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa
    11761 tacgataaag atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta
    11821 caaaacatca aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt
    11881 catggaaaaa atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa
    11941 tattatatta aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta
    12001 gaagaagcag gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta
    12061 aaagatctgg ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt
    12121 atgcgttggt attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat
    12181 gaaaaaatag aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg
    12241 aattttttag atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa
    12301 aatacaggaa ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg
    12361 gatttttcaa aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata
    12421 cttttgtaaa ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga
    12481 tttgattgca aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa
    12541 aaagaatgat ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga
    12601 atttagaaaa aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc
    12661 gtctaataat tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt
    12721 gaaagatgat gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct
    12781 agaaagtgat gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc
    12841 gtattatcaa gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa
    12901 cgctcgtaga tacgtactga aagggaattt attccgaccg caagacaata caacacaaga
    12961 tcaaatcaat aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg
    13021 tgcggtattt caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag
    13081 caattcaaga gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt
    13141 tcacgttccg aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc
    13201 ttttttaatg ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag
    13261 tctctatgaa gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat
    13321 caagattact agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt
    13381 atttacaatc aacgaaggaa gagagcgcgt tggaaatgat cgctctgata aggcgatggc
    13441 agatgaaata tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga
    13501 cgacaatgaa aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc
    13561 agggatttat tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata
    13621 ttttggatag tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag
    13681 acgtatttca agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg
    13741 ttgtgattaa cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta
    13801 taaaaatcta caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa
    13861 atgtcgatga cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg
    13921 cttcatataa aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag
    13981 aatcgtttat ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg
    14041 cagaaaatag ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg
    14101 acgttaaaga aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat
    14161 ttggacaagg agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag
    14221 cgaccaattg atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt
    14281 aagcttatct aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga
    14341 tcaattagta ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta
    14401 taacgaagta gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt
    14461 atttgaacgt gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt
    14521 tgttaacaca actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg
    14581 gggtaaactt tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat
    14641 gaagcagttc aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc
    14701 agtatggtta gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga
    14761 acaagcaatt gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag
    14821 caaacaaact agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc
    14881 tgtaactatg ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga
    14941 tccgatttat cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta
    15001 ctggtctaaa gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt
    15061 attgccagta ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat
    15121 tggggtagcc tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga
    15181 tgaataccat tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca
    15241 acctaaacgt aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac
    15301 aactacaaaa ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta
    15361 tattctttgt cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa
    15421 tctgaagaaa ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat
    15481 tcctattttt tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga
    15541 cgacaaagag tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga
    15601 tcattcgcgt gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag
    15661 aattaatttg cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag
    15721 ttatctaagt cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat
    15781 ggcaaacatt gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg
    15841 cgcacagtgg gttattaacc gccctaaagc gaattattgg aaaaaggttt atctggattc
    15901 taatcggcta tatcgctacc ttagagggca aaaaacaaat ataaaaatct ggacagccga
    15961 gatgtgggca cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga
    16021 attagacttt tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca
    16081 caatgctgga gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc
    16141 tacgcctttt gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt
    16201 taaagcaatt aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata
    16261 gctgtgaagg ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg
    16321 atagctaaaa atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct
    16381 gaaagcttag aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta
    16441 gatgagtttg aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta
    16501 caagaatata ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa
    16561 cgatggttgg ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat
    16621 tggtgtagaa gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag
    16681 tgatataaca gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt
    16741 tcatccaatc gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg
    16801 ttacaacgtt atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt
    16861 gggtgaatat ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg
    16921 ctatcaaaac ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg
    16981 aggatgagct acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga
    17041 tcactgagcc aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata
    17101 gagaagattt aacaggagat tcactagata ttatttcatt gattcaaaat cgttcgattc
    17161 gttttcagag aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc
    17221 aattggtatt tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg
    17281 gagctaaaaa taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag
    17341 agcgaacgac tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg
    17401 attacagaaa aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa
    17461 aatagtcgtc cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga
    17521 aaatttgagt atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg
    17581 gacgaattta tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt
    17641 cgtcaattcg ataaattaaa taaataggag gaacaacaat ggctaaaaca acaacagtaa
    17701 caacttttga caatataagt attaaaagaa tttcttttaa ttttaagaat gcaacaaatg
    17761 caatttcaac tgattgtaat ggacaattag atggcgaaac agaaatgcag agaattgtaa
    17821 aaaaatgcgg ttcaacagaa gtaaaatcga aatctaaacc aatcaatatg acggtaacaa
    17881 ttactgcaca tgtaccgatg gaagtttatc gacgcttcaa tgggttgaaa caagatgaac
    17941 gtattaaatc gggaatttac tcttatggtc ctgattccgt aggggaagat ttctctcttg
    18001 ctgcagagat cgtggatgat ttcgaagaaa aaaataagct gattggtatg ttagcatgca
    18061 cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta gctgcgttag
    18121 aactagaaac aaaagttatg caagatgaat ttggtaaatt ttatcatgaa gcaattgttg
    18181 cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct gatgtgatta
    18241 aaaaaacttc agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc
    18301 gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa
    18361 cctaaactga atatcaagaa attattgatg atcaatagag attttaacac agacgagttt
    18421 gcaaaaatga ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct
    18481 gtgtatattg cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc
    18541 gataaatggg attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa
    18601 caagcacgcg atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa
    18661 aagtaaaaat gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg
    18721 acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc
    18781 aaatggctta caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatccc
    18841 agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaaca
    18901 ttcatagcta acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact
    18961 aaaaatagag cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca
    19021 gacaagttag gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagtcaaaaa
    19081 gttgaagtaa ctagccaacg tttagccaat gccaaaaaat attatggaga aaattccacc
    19141 gaagttcaga aacttgagag agaactgatt aaccaacaaa cagcgcaaca acgtttgtca
    19201 aacgaaattg ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac
    19261 gagtctacaa tgcaacagtt ggatagtgaa caaaaaaatg ttcaagctag tgcttctctg
    19321 attgaatccg aatacaaaaa atggcaagca actgctggtc aatcagcttc tgaatccgag
    19381 aaattagcga aagcccaaga atatgtttct caacaatctg aaaatgcaga gaaaacgata
    19441 gatatcctga gacgacagtt agaagctaca cagtctgagt ttggcgctac atccacagaa
    19501 gcaatgcaga tggaggcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa
    19561 gctgctaaaa atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat
    19621 aatctaatgg aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg
    19681 aaacaagcag tggactctgc taatagtgta ggtagttccc agagtaaaat acaagctaat
    19741 tttggtttga ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat
    19801 aaaggttttg gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat
    19861 ttaggcgatt taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa
    19921 aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat
    19981 ttcggcttga gcgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta
    20041 gataaaacgc acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg
    20101 ggatattcag ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat
    20161 aaccttgata aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga
    20221 tttgagcaaa acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa
    20281 aatggcggag gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg
    20341 gatggtcaat acgaccaatt aaataaagct tctacaattt ggtctgcact tggcgaagat
    20401 aacgcgatga aagttgtcca atctttgact gatgttaacc atacatttga tgatgttagt
    20461 ggatctgcac aaaaaatgaa tgaagattct actactccgt tacaagaatt aaacggaaaa
    20521 atagctgaat taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa
    20581 ccagtaattg attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta
    20641 caggattatg ccgtagcgat tggcggattg actgctgcat ttactttatt aatgccaata
    20701 atagttggct tcatggctct aggtggtcct actacattaa taataggagc agttattact
    20761 gttattgctg gagttatagc aattataaaa aactggggcg caattactga ctggtttaag
    20821 ggaatatgga gtaaattcac tgattggttg ggtggtactt gggaaagtat aaaagaaggt
    20881 gcctcatcag tttgggatgg agttaaagaa acctggtctg gatttgtaga ttgggttcaa
    20941 gatatttggc aaggagtttc tgattggttt ggagagctat ggagcggatt agttgaagga
    21001 gcttccaaca tctggcaagg agtccaagag acttggcaag cattcgttga ttgggtttca
    21061 aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt
    21121 gttcaaatac catggacctt aataacgtca ttgattcaag ccggtattaa tattatcgtg
    21181 ggtatttttg atgtagctgg acagttatta ggcgcagctt ggcaagctgt ttggacacct
    21241 atttctgatt tccttaaaaa cacttgggat actatgacac aatgggtaag catcgcttgg
    21301 aatggaattg taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt
    21361 atatggacag ctattagtac tacggcttca aatatttgga attcaattag tgcaacagct
    21421 tctagtattt ggaacagtat caagaataca atcactagct tggtacaagc agctgctaca
    21481 gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg gaattcaatc
    21541 agctctacag catcaaatat ctggaatagc gtgactagta gtataagcaa tgctataaac
    21601 gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat cagcggaatt
    21661 tggaacggta tcaaaaacac tgctttgaat ctttggaatg gaattacaag cactattagc
    21721 tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct aacaggtatt
    21781 gtttccgaca tattcaataa tgttaaaagt acaattgcta acatttggga aggtatcaaa
    21841 aagactgtta gcgctccaat tgattggatc agagataaaa tcagtagtat ctttgataat
    21901 ttgaatattt ctataccaca tattccgtta ccacatttta aattgagcgg agaattcaat
    21961 ccattgaagg ggaaaatccc aacgttgggt gttgattggt atgcgaaagg tagtgtgttt
    22021 aattctccga atattatcgg tgtcggcgaa gcaggacctg aagcagtttt acctttgaaa
    22081 agatctgtgc tgcaagaaat tggtgatcgt atcttgagta gcacatcagt ttcatctagg
    22141 gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga tggtaacgca
    22201 gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac aaaagttcaa
    22261 aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt ttttagtatt
    22321 aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga tttcctgatt
    22381 atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa agacagagag
    22441 ttgaaacttc tgttttagga aggttaggtg gcttagtaca agattactcg tttgaagaca
    22501 tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa gcgttcaagc
    22561 aatcgtttta tatcatgcgt cattggttaa attatgcaaa gaaattagaa ttctctgatg
    22621 atcccaacgt ctattatgtt atccagacta tcgatattgg ggatgcagaa aacgatattg
    22681 ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga gttcaagaag
    22741 atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat agtttagaag
    22801 aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc atcttaaatg
    22861 attatgtgtt tagttttgaa ggcttagtag taggaactga cgtagtcatt gatagtgatt
    22921 tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc aacaaaatga
    22981 agaccatgca atatccgaca ttgcaagtgg atattaatta ttttaattgt actggtttga
    23041 gcaaaataca aatttatcgt aatgggttaa ggtaggtgaa atagatgatc gataatttaa
    23101 taactattta cgataaaaac gacgcgaata atttagctga acatttatat gatacgcaag
    23161 gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaatta aacggagccg
    23221 agatatttca gggtacttat ccaataagcg gaactaatgc agatttgatt gtagaaggac
    23281 gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg atttattatg
    23341 caaagacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt ttcaatgata
    23401 taagaaaatc ggtgttgaat aaatatgaca gtggaacaga aaagatcact gctagtcagg
    23461 catggcaaaa cgcaaaaact ttagcgaaac cagttattcc ttcacagttt tctttctcgt
    23521 cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta gaattctttg
    23581 gtggaaaaga gggatctatt ctagatcgat ttcatgggga atttctaaaa gataataaca
    23641 cattacgtca tgaaaaaagg ctaggcacgg atcataaaat caaagcgatt tatactaaaa
    23701 acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga gtttatccat
    23761 tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa gttattttca
    23821 cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt aaagacaaag
    23881 cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca aacatagata
    23941 aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat caaagaggct
    24001 atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat gtgtatcatc
    24061 cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat gttctaacca
    24121 attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa aacacagaga
    24181 ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacggt ggcgaaatca
    24241 gtgatttact caatgatatt gtagatcatc aaactgatat gattactggt caaaatggtg
    24301 gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg gacacaccag
    24361 ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt ttttctaaaa
    24421 ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc aatgcctcgt
    24481 ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta attagtgatg
    24541 gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca aaaaaagtta
    24601 acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc ggtattgtat
    24661 catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt aacttggttt
    24721 ttagtacgtg ggataatggt aataacagac cgtttctatc ttttggtgcg cccaatttca
    24781 ggtatagcaa tgctagttat gtaacttctg gcgacggtag ttctttaagc attaatggta
    24841 gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt actagtgatg
    24901 gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa cttgctaggt
    24961 ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact aaaaactcaa
    25021 ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact ccagaatatt
    25081 atttcgctga ttatggggaa gccgttacag gtgacgatgg taaagttcgt gttgatattg
    25141 accccatgtt tgctgaaaca gtaaatctaa gtcggtatat gacacatgtg acacctacag
    25201 aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt gaaactagta
    25261 agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat gaagatatta
    25321 gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt ttttaaaacg
    25381 aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag atttcagcaa
    25441 agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga ttacggtgac
    25501 ggttaaaccg tttctaaatg gcttgcagat ggatacgagt ggcggaacat ttactttaaa
    25561 aggaacaaca ccatctaacc gttacgtaga taatgttgca actagtgtaa ctagtgaaga
    25621 agtcacgttt tctcttgatg gcacatttat gagtgaagca ggatattata aacactgcta
    25681 cgtagaatat agaaaagaca atcaaatttt aacaacgcaa gatatcattt ttttctcact
    25741 aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat tagaagagtt
    25801 gattcgaaag tacaacgaaa cttttgatgc ttttatggct gaaatcaaag gtagagtgga
    25861 tagcttaaat caacagatta ctgatttaac tggtcaagct aaaacgctac aagacaagtt
    25921 agatgctctg aaagaagaaa tttctaagtt aggtaactta caagtgatgt acagtaacag
    25981 catcgatttc ggggaatatg attatagtgg gaatccgaat ttaatgtcca aactaaaatc
    26041 gagcgatttt aacgttggtt accacgggtc actaacttcg gataacgaaa agctacattt
    26101 tacttctgat ggtacaggaa gcattattat gtttacgcgt attaatacac ctcagcttgc
    26161 tagtgggaaa acctatactc tgagtgcgaa agttcgattt gatgaaggaa ctacaggagc
    26221 tattgataaa ttacgtttgg tgtatcgtac atcaccagga gaaaagatat tattggaagc
    26281 aaatagtaca aatattacaa cagatgatgt agggaaagaa ataacaatca aaggtacagc
    26341 taacgttaat tatcaaatca caaatttaga tcgattttat atgagtatta gctttgttga
    26401 cagggataaa ataaatggcg gatttaagtt gtacgacatc aaaatcgaag aaggctcaac
    26461 agccacccca tatcagccaa acttacttga tgacccttac tggctaggta aagcgccttt
    26521 gggtgagaat attgctaata agtctgttac gtttccaatc aaatctagcg cctacagcct
    26581 ataccaagct aacatggaag aagaatttgt tttaggtcaa acctatacaa ttaccatgaa
    26641 ggcaaccaaa ccacctatcc aaacgttcat tgtttacaac gaggatagtg gagattatag
    26701 atatggtaac ttagagccag tagaagggtt agttgataca tggggtctga cttttacacc
    26761 gcagaaggtt ggagttaact accccaaacg gttaactatt atccagtatc cacaatcaac
    26821 agtaggcgca tgccggatcg actggctcaa gattgaaaaa ggcgacacac gaaccccgaa
    26881 tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc caaaagatta
    26941 tgtttgggat ctagcaccag aatatgtcga agataacttg gccacagata ttaaaatttc
    27001 tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga ttaattcgca
    27061 gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa caaaagcgaa
    27121 tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca ttaatgatac
    27181 agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc tttatcgtga
    27241 aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg gtggaaatga
    27301 ttcaacgata ctaacaattc cagaaggcta tcgg
//
LOCUS       DACCWV010000021        27211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCWV010000021 DACCWV010000000
VERSION     DACCWV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.021"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(161..445)
                     /locus_tag="IVW84_000830"
     CDS             complement(161..445)
                     /locus_tag="IVW84_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917045.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IVW84_000831"
     CDS             complement(463..1353)
                     /locus_tag="IVW84_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7917046.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IVW84_000832"
     CDS             complement(1373..1591)
                     /locus_tag="IVW84_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917047.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3241)
                     /locus_tag="IVW84_000833"
                     /pseudo
     CDS             complement(1561..3241)
                     /locus_tag="IVW84_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            3508..4281
                     /locus_tag="IVW84_000834"
     CDS             3508..4281
                     /locus_tag="IVW84_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917048.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4362..4931)
                     /gene="thiT"
                     /locus_tag="IVW84_000835"
     CDS             complement(4362..4931)
                     /gene="thiT"
                     /locus_tag="IVW84_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7917049.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(4997..5099)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5105..6076)
                     /locus_tag="IVW84_000836"
     CDS             complement(5105..6076)
                     /locus_tag="IVW84_000836"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7917050.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6170..7543)
                     /gene="glmU"
                     /locus_tag="IVW84_000837"
     CDS             complement(6170..7543)
                     /gene="glmU"
                     /locus_tag="IVW84_000837"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7917051.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8046..8864)
                     /gene="purR"
                     /locus_tag="IVW84_000838"
     CDS             complement(8046..8864)
                     /gene="purR"
                     /locus_tag="IVW84_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7917052.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9031..9855)
                     /locus_tag="IVW84_000839"
     CDS             complement(9031..9855)
                     /locus_tag="IVW84_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7917053.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9828..10508)
                     /locus_tag="IVW84_000840"
     CDS             complement(9828..10508)
                     /locus_tag="IVW84_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7917054.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10531..11469)
                     /locus_tag="IVW84_000841"
     CDS             complement(10531..11469)
                     /locus_tag="IVW84_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7917055.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11714..12565)
                     /locus_tag="IVW84_000842"
     CDS             complement(11714..12565)
                     /locus_tag="IVW84_000842"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7917056.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12719..15292)
                     /gene="mprF"
                     /locus_tag="IVW84_000843"
     CDS             complement(12719..15292)
                     /gene="mprF"
                     /locus_tag="IVW84_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7917057.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15433..16239
                     /gene="proC"
                     /locus_tag="IVW84_000844"
     CDS             15433..16239
                     /gene="proC"
                     /locus_tag="IVW84_000844"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7917058.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16318..17874)
                     /gene="rny"
                     /locus_tag="IVW84_000845"
     CDS             complement(16318..17874)
                     /gene="rny"
                     /locus_tag="IVW84_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7917059.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18285..19334)
                     /gene="recA"
                     /locus_tag="IVW84_000846"
     CDS             complement(18285..19334)
                     /gene="recA"
                     /locus_tag="IVW84_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7917060.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19443..20684)
                     /locus_tag="IVW84_000847"
     CDS             complement(19443..20684)
                     /locus_tag="IVW84_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7917061.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20924..21502)
                     /gene="pgsA"
                     /locus_tag="IVW84_000848"
     CDS             complement(20924..21502)
                     /gene="pgsA"
                     /locus_tag="IVW84_000848"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7917062.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21711..22595)
                     /locus_tag="IVW84_000849"
     CDS             complement(21711..22595)
                     /locus_tag="IVW84_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917063.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23372..24658)
                     /locus_tag="IVW84_000850"
     CDS             complement(23372..24658)
                     /locus_tag="IVW84_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7917064.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24651..25919)
                     /locus_tag="IVW84_000851"
     CDS             complement(24651..25919)
                     /locus_tag="IVW84_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7917065.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26171..26632
                     /gene="mscL"
                     /locus_tag="IVW84_000852"
     CDS             26171..26632
                     /gene="mscL"
                     /locus_tag="IVW84_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7917066.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26691..27137)
                     /locus_tag="IVW84_000853"
     CDS             complement(26691..27137)
                     /locus_tag="IVW84_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7917067.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7735 a         5867 c         4722 g         8887 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc ttttttcata tctccttgta
     2701 aaatcaatgc gccgttcaac gcacctaccg aggtacctgt gataatctca aagttgatat
     2761 catgttcctt cagcgcctgc cacacaccaa tctgataagc accgtgcgct cctcctccac
     2821 ctaaaacaag tgctgtatga tagaccagct ttttatttaa ttgtgtggtt ccttgatagc
     2881 cgtttttatg aaaaatacgc aaccattctt ctgatagagg agattgaaat aaaaaacaac
     2941 agttttcttt aataaaaaaa cgagcgcaag cttccatata ccgaaaaaat tttttccatt
     3001 caaaagtttc accttcaatc aacagattat taaaaataac atctcctcta cttttagcaa
     3061 tagtaaaata gacggtatat tttttcgata ctgctttaaa agcacattgc ttttttgcag
     3121 aacgccaaaa agcgtctcta ctttcttgat gactttgaaa aaatggaagg ggttgttttg
     3181 cttgctggat aggcgaatac agcgacattt tctgtgaaaa gtaaactttt tttatctcca
     3241 tcgcgctctc ctttttcttc aagtatacca gtacgtcata aaaaggtcat gagtctcgta
     3301 ttcgactttg tagattccat aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata
     3361 caaaaatcac aaaaattaat tgaaaatgag agtgttttca atgaaaacgt gattttgtaa
     3421 tggaatttta aaataaaaga aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga
     3481 ttaagaggaa ataagaggag gaacatgatg actgagtcag tttatgtaca tattgatacc
     3541 acaagtaatg ctgttttaac aaaagggctg actgctactg actttgcaaa cagtatcgtg
     3601 cattttcctc aaaacttgct cttgcttgat ccatctgctt ctgctggaga atatgaatcc
     3661 catacagggc tgaaagtaat ccgtggtaca gaaaatattc agcgtttctt ttcctcttct
     3721 cgtaacaggc gtggattcga tgaattgaag tggatcgact ttactgattt aacgatgctg
     3781 aaagaattga cacctttaga aatttcagaa ttattatatt ttggtcatat gaaaacacat
     3841 cttcattcgc cgttttttta taaactacag aataattttg tatacttcga tcttaatgat
     3901 cagctgaatc ggatttatta tcgctattta gatgaatttt ataggatttt tgctagtaaa
     3961 ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca
     4021 cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc gtaatttgat gcaggaaggg
     4081 atcatttttt cttttgatca agttggtttg atcgatggta aatatcgtat cccaatctat
     4141 gtgattgaag attatatttg gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc
     4201 gctactttaa tctactctgt caccgatcga cactggcaat tggtagaaga tgccgatctt
     4261 tcttatctgc cattgaagta gatctatttc ttatcaaaaa ataaaaagga aacaagaaac
     4321 taggacatga ttctgtctca agtttcttgc ttctttttta tttacagctc ctgtcgttta
     4381 tctggcacaa aaatcgaagg agtcgtttgt tggatcccta ataaaacgat gatcgttaca
     4441 atagctgtac tcaacccgct ggcaccattc attacgaaag aaaagagcca aggattcatc
     4501 ccccatagtg catacgctcc ccagaaaacc actcctgcaa taaaatgcca aaaatatctg
     4561 gaaagtgcgc caatcaatgc accaaacacg atgctttttc taatttgttt cgtattatcg
     4621 tctacatagc ctttttggac atttgaagcc atgagccctg ctgcgcctgc aaaagtatag
     4681 gcaatcgggt actcaatcaa cacttgaacg acacttaaat aataaacttg tcccaacgga
     4741 aaatgtagga tgccccaaat gaaacctgcc agcatccctg cctttgtccc tcttcttaac
     4801 gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa aactgctgcc gatctggatc
     4861 ggaatcaacg acaacaccat cgctaacgct gcaacaatcg ttccttctac ccaaactcta
     4921 ttttttccca taaaaaaact tcctcctttg aataaaaaag tcacaaagga ggaagcagct
     4981 ttcgaaaata gtcaaacagc tgtttattca caattcctac gctcgcataa ccgatacagg
     5041 tacgaagggt ttagaaatag attcaatctc agcccaattg gactcccctt tgtgagttta
     5101 tttattattt attttttgtt tcaaacaatg gactaactgg tttgttttca tggatacgtt
     5161 tgattgcatc tcccattagt ccgccaacac ttacttcatc gattttgtcg attttacgat
     5221 cttctggaag atagatcgag tctgtaacaa ctagacgctc gatagctgaa tcttctattc
     5281 tttgtaaagc agggccggat aatactggat gtgtacatga agcatacaca ctagttgcac
     5341 cagcttcttt caaagcattg gcagccaatg tgatcgtacc tgctgtatcg atcatatcat
     5401 cgatcagtac acaagttttg cctttgactt gtccaatgat attcattact tctgcaacat
     5461 ttgcttttgg acggcgtttg tctatgatcg caatcggaga tttcaaaaat tccgctaatt
     5521 tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac gacaacatca tcgcctttga
     5581 ttcctttttc taagaaataa ttggcaataa gtggcgcacc catcaaatga tcgactggaa
     5641 tatcaaagaa accttggatt tgaactgcat gaaggtccaa agtcagcatt cttgttgcac
     5701 cagctttttg aatcatgttt gctactaatt tcgctgtgat tggttcccgt gcacgtgctt
     5761 tacggtcttg acgcgcatag ccataataag gcatgactac gttgattgtt tttgcacttg
     5821 ctcgtttcag tgcgtcaatc atgatcagca actccatcaa gttatcattg actgggctgc
     5881 tcgttgattg aataacatag acatgcgatc cacgaatact ttcttcaatg tttacttgaa
     5941 tttctccatc actaaattga gtaacagatg attttcctaa ctcgacgccg actgctgctg
     6001 caattttctc tgctaatggg cgattggaat ttaacgcaaa aatttttaat cttgggtcaa
     6061 agtaatgttt tgacatgggg acctccactt tctttttcac aactctagaa ttctacctaa
     6121 taaatagtag tcatattttt taaataaatc aagtgatttt gcaaaagtct tagtttagat
     6181 aagggagttt ttttgcatat ccttctttat tgacttgtct tgctctcgca attgccagcg
     6241 catattctgg aatattgtct gtaattgttg acccggcagc aatcgaactg tttttagcta
     6301 cctcgacagg tccgatgata ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg
     6361 tatgatgttt gttctttcca tcatagttga cgaagacaac accacatccg acattgattt
     6421 cttctcccag cgtagcatca ccaacataag tcaagtgacc gactttagtt cctttgccga
     6481 tttgagcatt tttcacttcc acaaagtttc cgatatggac gttttcgcca atttcagctt
     6541 ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa
     6601 tgactgagtt ttcaatgacg acgtgatcct caatcctact gtctacgatt tttgaatgtg
     6661 acccgatgac acaatcagaa ccaatgactg tatttccttg gatttgtaca cctgcttcga
     6721 tcaatgtgtc tggaccgatt tctacgcctg catcgatgta tgtcgttgct gaatcgacaa
     6781 agcttacgcc attgaccata tgcatctggt taattcgctt tctcatgatc tcatttgctt
     6841 tagctaacgc gatccgatca ttgactccca ttgattcttc aaaatcatcc gtttgataag
     6901 cagcaactgt atggccttct tcttttaaga tttcgatgat atctgttaga tagtattcac
     6961 cttgagcatt gtttgttcca actttgctta acgcatcgaa caaagcttcg ttgtcaaaac
     7021 agtaagttcc tgtattgatt tcttgtacaa gagcttcttc tggtgttgca tctttttgtt
     7081 ccacgatttt ttctacgatc ccaatatggt cacggatgat tcgtccatat cctgttgggt
     7141 tttctgcttg tgccgtcaag atcgttgcac ttgcattttt tccttggtga tattcaaaaa
     7201 gattgttcag cgtttcagta gtcaataatg gtgtatcgcc actgataact aacgttgttc
     7261 ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt
     7321 ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt
     7381 gtccaacgat cgtaatcact tcatctggtt tcgtttcact gacccgatta atgatatgtt
     7441 ctaccatagg tttaccagaa acgggatgta aaactttata taattttgac ttcatgcggg
     7501 tacctttccc tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc actcctaaat
     7561 atagtttgct gatttcagtt tatcccagta tacccatttt ttcaacaaaa caagtgattt
     7621 tcttttatcc cagcgttttc tggataaaaa aatagataga ccataacggg cctatctatt
     7681 tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt cacactactc ataaaacatg
     7741 aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt aaacggtagg cttacaacct
     7801 cttcattaaa cgtttttcaa tcagctgacc ttcggtatta gctcaataaa cggcaaaata
     7861 tgaggagcaa tttttccgtt tattgagcta atactcagga caggacgctg atttcacaac
     7921 ctctagataa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa
     7981 tgtggaaagc cattttcaca aaatttttct tactactcga aactaagcag ctttttcaca
     8041 acctcttatt ctgaaaaata atttcctggc acaaccgtaa tatttttcgt ttgagtatct
     8101 acatctttga cataaagcag tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt
     8161 gattcggcaa atacagtgat gccaacaagt tctgattcga attcttcaat caagctcttc
     8221 attccgttga ctgttccgcc gcctttcatg aaatcatcca ctacaaggac acgtgaacca
     8281 cgtttcaaac ttctcttaga cagttccatc ttttcaattc tttcagaaga tccagacaca
     8341 tagttgacac ttactgttga tccttctgtt atttttgagt cccggcgtac aataacgaat
     8401 ggcgcattca gataatagga cacagcttgg gcaataggta cgccttttgt ggctacagtc
     8461 attacagcat cgattttttc accaaggtat ttagaagcaa tgattcttcc aacttgacgc
     8521 agcagttcag gttctcctaa caaatcagag agataaacat atcctcctgg aagcagacgg
     8581 tcttgttctg atagacgttc tgctagagat tcaatatatt ttttcgcctc ttcatatgaa
     8641 atttcaggga tgaacaatac accacctgca gcacccggga ttgtttccaa ggttccatac
     8701 cctcgttctt taaatgtttt cttgatgatt cccaaatctt cactgataga agatttagca
     8761 gattcataac gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg attcaataaa
     8821 tactgtgtca tgtcaactag acgttcactg cgacgtactt tcacctattc caactccttt
     8881 taagaaattt tatcgtttta cattatataa gtttttcttc aaaaattcga gcattttatg
     8941 aaaaaataag aaaatagttc ggtatttatt aaaataaaaa gaagcctata aaaaattttt
     9001 cgtttttata aggcttcttt tctttataat ttagttattt tttcttctga aggcaaccaa
     9061 tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg tgattgttgc
     9121 tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc ctagaaatcc
     9181 tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca taccgattgc
     9241 tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga tcatcacagc
     9301 aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg gtagcccatc
     9361 cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga ataaaaagaa
     9421 caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc atgtaatggt
     9481 cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc cgctcaaatt
     9541 catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg agatttctga
     9601 ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata caacgactag
     9661 catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc ctgctaaaga
     9721 gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa acacacctag
     9781 catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca tgaattcata
     9841 tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct gtccgcatac
     9901 tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg gacatgcgca
     9961 ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc tgttgttggt
    10021 tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact gattcgctgc
    10081 ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat ccctacagct
    10141 tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa ccagcggcct
    10201 cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc tgcattgaaa
    10261 gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg atttttctca
    10321 gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt caccaaggtt
    10381 gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc tacatgataa
    10441 gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga tatttcgata
    10501 taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta aagctttcag
    10561 atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat tagtcaagct
    10621 ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg tctcagctac
    10681 cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct tcacaaattc
    10741 ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc
    10801 tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa
    10861 tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg cttcaagctt
    10921 tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat atttttcgcc
    10981 taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga gccaaacatg
    11041 tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc
    11101 ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct gtttgctatc
    11161 cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat acacgaaaac
    11221 atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat gcggctccgt
    11281 tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc
    11341 atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg cttccttgtt
    11401 cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg tcaaaagata
    11461 ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt tattaacgtg
    11521 aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt ctgatttaag
    11581 catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc tagccatttc
    11641 actacaataa aaaactgaag aataccattt taggattcct cagtttttca tcacctattt
    11701 tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta
    11761 acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc
    11821 cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat
    11881 ggatgactgg atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca
    11941 tctgctgata atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga
    12001 tggcttgcgt atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga
    12061 ccaacacaac ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac
    12121 cgacaaatgc cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga
    12181 tttctgccag ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag
    12241 cagctgcaca gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa
    12301 caaagatctt gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat
    12361 aaacattgtt ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt
    12421 cttctgttat ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt
    12481 ccaactcatg atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg
    12541 ctggcgcttt ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta
    12601 ttctatcata gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat
    12661 cgtttcaaca gctttcacag aattttctct tcataatcca aacaagttct agtgattctt
    12721 ataattctgt gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca
    12781 gactgagtag ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac
    12841 gtccttccca atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga
    12901 aaaaattacc ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg
    12961 tttctccaac attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct
    13021 cgtatacaat aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta
    13081 agacccgcaa caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc
    13141 caacgacttt ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat
    13201 caaatcgtcc cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt
    13261 catttacagc ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt
    13321 ctttcatcaa acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta
    13381 gatcaacaat ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca
    13441 ctacgtagct ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt
    13501 tcataaactg attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa
    13561 caaagcattt gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt
    13621 ggtaataaaa ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt
    13681 atgtttccaa taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat
    13741 cttcagctaa atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa
    13801 gccctgagaa ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac
    13861 cgactaaaaa gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga
    13921 acaagctagc atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt
    13981 cttttctgga taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag
    14041 ataacgtacg tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa
    14101 atgccttttt gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca
    14161 atagattcac tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc
    14221 gactgattgc agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat
    14281 agacagcact tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat
    14341 ctaaaaatcg attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa
    14401 aaggtagaac ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc
    14461 gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg
    14521 ggaccatcgt cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca
    14581 tcgaaatatc tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc
    14641 attgtccaaa ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca
    14701 atgtctttct ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga
    14761 aaagccagat ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa
    14821 atgtaagaaa cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag
    14881 tggctaaaac catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc
    14941 ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa
    15001 gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc
    15061 aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg
    15121 tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa
    15181 tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa
    15241 tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc
    15301 attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt
    15361 ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa
    15421 ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt
    15481 gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat
    15541 tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag
    15601 gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa
    15661 gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga
    15721 aaatcattaa cagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg
    15781 atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct
    15841 gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat
    15901 cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc
    15961 gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag
    16021 acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt
    16081 gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca
    16141 ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg
    16201 acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg
    16261 ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta
    16321 tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata
    16381 atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc
    16441 cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact
    16501 tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta
    16561 gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc
    16621 tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt
    16681 gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga
    16741 tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc
    16801 taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac
    16861 cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt
    16921 gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc
    16981 gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg
    17041 atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat
    17101 tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc
    17161 agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat
    17221 ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca
    17281 gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg
    17341 ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt
    17401 catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg
    17461 ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga
    17521 actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt
    17581 acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt
    17641 actttctctc agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc
    17701 taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc
    17761 agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc
    17821 taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc
    17881 cacctccata ctatcttttt catttttatc atctatacag cttctatgat aaattattct
    17941 aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct
    18001 aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc
    18061 attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg
    18121 agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc
    18181 actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga
    18241 gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa
    18301 ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa
    18361 gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt
    18421 ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt
    18481 ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct
    18541 aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg
    18601 actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga
    18661 cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct
    18721 ggattcccaa acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt
    18781 tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt
    18841 aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca
    18901 actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct
    18961 agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat
    19021 ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca
    19081 tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt
    19141 ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac
    19201 gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata
    19261 atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta
    19321 cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta
    19381 tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt
    19441 tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat
    19501 ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat
    19561 gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact
    19621 ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc
    19681 tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga
    19741 aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca
    19801 ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt
    19861 acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg
    19921 ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga
    19981 gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt
    20041 ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca
    20101 gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct
    20161 tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac
    20221 tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct
    20281 aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc
    20341 agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat
    20401 ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg
    20461 tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct
    20521 agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg ataataaact
    20581 tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt
    20641 cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt
    20701 atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac
    20761 aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt
    20821 cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact
    20881 cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga
    20941 acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag
    21001 cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa
    21061 acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag
    21121 ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac
    21181 agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag
    21241 cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa
    21301 ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa
    21361 taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc
    21421 aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag
    21481 ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc
    21541 agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt
    21601 tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc
    21661 cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg
    21721 tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt
    21781 acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca
    21841 tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga
    21901 taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca
    21961 agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct
    22021 cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa
    22081 gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact
    22141 ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata
    22201 tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt
    22261 gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact
    22321 gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca
    22381 tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc
    22441 acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt
    22501 ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct
    22561 tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg
    22621 attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag
    22681 tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca
    22741 agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga
    22801 gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta
    22861 ttagctcaaa aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca
    22921 ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    22981 gtaataagtc caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt
    23041 acttgatgca gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc
    23101 ttcggcaata agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt
    23161 atttcttgaa gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt
    23221 taatataagt tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac
    23281 acaactaaga ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg
    23341 aaatttaaga cttttcctca taaatactgg tttattttgg tctcatgtaa aactcgctga
    23401 aggcctcttc acttataaat gcttccccag ctgccagtac gtctttcatt tggatcgaat
    23461 cgatgatttc aggaagatca aataaagttc gatcgccaaa taaactttgc gtaaattgat
    23521 tggcaatata ttcgatcgaa tttaatgatt ggaaatattg tccaagcatt ttcttcttca
    23581 gcaaatcgag atttgtttct gacacttccg gatcatcagc gaatccaaga atgatttctt
    23641 tgactttttc tgctgctttc tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa
    23701 attcacgatc caagctgaat tcaaaaccaa agctgtcatc gataattcct tgattgtaca
    23761 tcgctagata gtttcgagaa gtattgccta aaatcatttg gaacaacaga ttgattgctg
    23821 ttttatagcg taacagttct gttccttctt gcggcaatgt gtctaatcct ttaataccaa
    23881 gtacaaattt atctcgtgta atggctgctt ccaatgcact ttgtttgatg atttcttttg
    23941 tgttttcagg gaaataacgt acaatttctt gctttggagg gaaatttttt gcttcttggt
    24001 tttcccgaat aagtttcatc aatttctcag gctccatctt accaactaca aataaaacca
    24061 tattgcttgg ctgataaaac gtccgatagc acgtatacaa gtcttgagct gtgattttgt
    24121 cgatactttc aaccgtgccc gcaatatcga tatgcaatgg gtgtgtagga tacaagttat
    24181 ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc atacatttgg atttcctgac
    24241 cgatgattcc tttttccttg tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat
    24301 cgatcagagt cgtcagattc ttttctacct gatccgtcgt agaaaacaga tagctggttt
    24361 tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt cccaaataat tgaaagacat
    24421 caccatcttc tttttcaaac aacttatgtt ctaaaaaatg ggcaattcca tctggaactt
    24481 tcaccttttc tttttcacca tagggaataa attcgttatc gatcgaacca taattcgtac
    24541 tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt
    24601 ttggcagtac ttcatgataa agtgtttcat tgatttgctc atattctgtt ttattcattt
    24661 gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga tttgctcagc tacttgttgt
    24721 atctctttga tcgttacgct ctccatacgt ttcaaccatt cttcatcaga taattttgtt
    24781 tccggcagcc agctgtccaa aaaagaagct tcgattgccg cttgcggaga gtctagagaa
    24841 agcagatatt ggtttctcag cattgctttt gtctgagaca gttccaactc tgttatttca
    24901 ccatttcgca aactttctaa ttgctcgtga atcagatgaa gtacttgatt tcggttcttt
    24961 tcatcgatac cggtttggac actcatgaat cctcggaacg tatccacgct actagaggca
    25021 taataagcta gactttcttt ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg
    25081 ccaaataaac cgttaaagac cattagcgca aaacgttctg gttcatcgta atacacattt
    25141 gtctgataag ctaggttcag ctttgcttga acgattggct cacgtacttg tcgttccttc
    25201 acgatattca cttgaggttg ggtataaaac agatctggat aaactgtttc ccgcgactca
    25261 aatggtaatt gcccaactgc ttcagctgct ttgtccggat caacatcgcc gacaacaaaa
    25321 atgtcgattt gatcatttgc catcatttct tgatagtagg acgctagcga actagcagtg
    25381 atcttgtcca aatcttcgac tacgcccaac cctgggatct tttgttccgg gctgttttca
    25441 aaataaagtt cttgcaaggc ataggaagca aaagtttgtt tatcttcctt caaactttcc
    25501 aaatatagac gcagattgtt cttttccaag tcaaacgtca actgatcaaa ctgatgattt
    25561 ttaatatttg gataaaataa aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt
    25621 ggatcattga catattcacc attcacaaaa ctgatccctg cgtttagcca atgtaaattt
    25681 cctttttttc taactgataa tccaaagctt gcgccataaa gctctgcaag ttgcgcactt
    25741 aactttgtct gatccggata attcaaacta tttgtttcca acatgctgct gagaagtgaa
    25801 cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt
    25861 ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt ctgccaattg atacaccata
    25921 tttatatgct ccttttcaca gttgattgcc cgcttactct tttaatgggt caacctgcct
    25981 tttttactta ttcctctgct tgaaatttat aagttcaggc aattcgccgt tttttctaaa
    26041 tgatgttaca ctttatagta tagcatagat gttttttatc aaaagaaaca gacggctaaa
    26101 agcgtatatt gtttttttat tttttatttt tcgatatact aaagacaaat aaaagaaggg
    26161 agggtcattc atgatcaaag aattcaaaga gttcatcatg cgtggagatg tcttggatct
    26221 tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg
    26281 cttgatcaca ccactaatag gttggtttgt cgcaatgatc acaggtactt ctgatttaga
    26341 aggagcactt tcaattcttg actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa
    26401 cgttattagt gctatcatta catttctgat cactggtttt gtcttattct tagttgtcaa
    26461 agcggcaaac cgtgcgaaga atttacgctc caaagaagag caagtggaag tggaagaaac
    26521 tcctactgca gaagattatc ttagtgacat acgcgatctt cttgccaaac aagtggcaga
    26581 agacaataca catccagtag agcaaacaaa tctttccgat acttctaaat aataaagaat
    26641 ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt ttttagattc ttaacttgct
    26701 acttgttcct ttttcatgtc gatatgtgga atatcatctt ccaaatacac atctgagatt
    26761 gccttgaatc caaacgactc ataaaaagct tgtaggtatt cttgtgcgcc gatcacgatt
    26821 ggtttttctg gaaaattttt ctggatcatg ttaatcgttt cttgaacaat ctttcttcct
    26881 aaaccatctt tacggttgtc ctgatgaaca aggacacgtc caaaatggat actctccgct
    26941 tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag tatcttgaaa aaacgtatgc
    27001 caagcttgtc catcaatgtc atcaacttct tgataaggac actgttgttc cacaacaaaa
    27061 actgcgattc gttctttgac gatcgcgtaa aactctgctg ttgacaattc ttcaaatttc
    27121 tttacctgat attccactaa gcaaacgccc cctgcttttt tattcttagc ataccgattc
    27181 gcacacaaaa aagcaaacga accttacgaa t
//
LOCUS       DACCWV010000022        27010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCWV010000022 DACCWV010000000
VERSION     DACCWV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.022"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            14..646
                     /locus_tag="IVW84_000854"
     CDS             14..646
                     /locus_tag="IVW84_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917068.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(780..905)
                     /locus_tag="IVW84_000855"
     CDS             complement(780..905)
                     /locus_tag="IVW84_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7917069.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1039..1932)
                     /locus_tag="IVW84_000856"
     CDS             complement(1039..1932)
                     /locus_tag="IVW84_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7917070.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2114..3040)
                     /locus_tag="IVW84_000857"
     CDS             complement(2114..3040)
                     /locus_tag="IVW84_000857"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7917071.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVW84_000858"
     CDS             complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVW84_000858"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7917072.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVW84_000859"
     CDS             complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVW84_000859"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7917073.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVW84_000860"
     CDS             complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVW84_000860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7917074.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVW84_000861"
     CDS             complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVW84_000861"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7917075.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11311..11745)
                     /locus_tag="IVW84_000862"
     CDS             complement(11311..11745)
                     /locus_tag="IVW84_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7917076.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11872..12510
                     /gene="plsY"
                     /locus_tag="IVW84_000863"
     CDS             11872..12510
                     /gene="plsY"
                     /locus_tag="IVW84_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7917077.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12642..13517)
                     /locus_tag="IVW84_000864"
     CDS             complement(12642..13517)
                     /locus_tag="IVW84_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7917078.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVW84_000865"
     CDS             complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVW84_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7917079.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVW84_000866"
     CDS             complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVW84_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7917080.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVW84_000867"
     CDS             complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVW84_000867"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7917081.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVW84_000868"
     CDS             complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVW84_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7917082.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVW84_000869"
     CDS             complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVW84_000869"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7917083.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVW84_000870"
     CDS             complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVW84_000870"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7917084.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVW84_000871"
     CDS             complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVW84_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7917085.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21968..22735)
                     /locus_tag="IVW84_000872"
     CDS             complement(21968..22735)
                     /locus_tag="IVW84_000872"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7917086.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVW84_000873"
     CDS             complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVW84_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7917087.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23848..24072
                     /locus_tag="IVW84_000874"
     CDS             23848..24072
                     /locus_tag="IVW84_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917088.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKQVVL"
     gene            24069..24245
                     /locus_tag="IVW84_000875"
     CDS             24069..24245
                     /locus_tag="IVW84_000875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917089.1"
                     /translation="MISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFSLEVQ
                     DLDGNTLEPVWMKM"
     gene            24482..25024
                     /locus_tag="IVW84_000876"
     CDS             24482..25024
                     /locus_tag="IVW84_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7917090.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="IVW84_000877"
     CDS             complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="IVW84_000877"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7917091.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25810..26037)
                     /locus_tag="IVW84_000878"
     CDS             complement(25810..26037)
                     /locus_tag="IVW84_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7917092.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="IVW84_000879"
     CDS             complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="IVW84_000879"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7917093.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26572..>27010)
                     /locus_tag="IVW84_000880"
     CDS             complement(26572..>27010)
                     /locus_tag="IVW84_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7917094.1"
                     /translation="FYLENEALLNGTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLS
                     IGTLSLPIKDVMNMIKRNYKLPDWIEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVN
                     DDIRFSLYLPKDTSDASNNSNTNKTSTSSTESKTKENKKSSDE"
BASE COUNT         7140 a         5707 c         4650 g         9513 t
ORIGIN      
        1 atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt
       61 ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc
      121 gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga
      181 aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg
      241 tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc
      301 atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt
      361 cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc
      421 actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt
      481 tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac
      541 gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat
      601 gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca
      661 taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat
      721 tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc
      781 taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt
      841 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
      901 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      961 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
     1021 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
     1081 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
     1141 ggccatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
     1201 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
     1261 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
     1321 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
     1381 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
     1441 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
     1501 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
     1561 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
     1621 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
     1681 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
     1741 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
     1801 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1861 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1921 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1981 taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct
     2041 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     2101 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     2161 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     2221 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     2281 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     2341 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     2401 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     2461 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     2521 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     2581 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     2641 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     2701 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     2761 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     2821 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2881 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2941 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     3001 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     3061 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     3121 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     3181 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     3241 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     3301 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     3361 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     3421 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     3481 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     3541 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     3601 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     3661 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     3721 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     3781 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3841 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3901 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3961 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     4021 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     4081 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     4141 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     4201 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     4261 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     4321 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     4381 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     4441 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     4501 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     4561 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     4621 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     4681 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     4741 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     4801 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4861 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4921 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4981 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     5041 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     5101 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     5161 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     5221 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     5281 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     5341 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     5401 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     5461 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     5521 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     5581 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     5641 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     5701 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     5761 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     5821 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5881 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5941 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     6001 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     6061 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     6121 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     6181 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     6241 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     6301 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     6361 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     6421 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     6481 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     6541 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     6601 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     6661 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     6721 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     6781 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6841 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6901 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6961 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     7021 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     7081 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     7141 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     7201 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     7261 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     7321 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     7381 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     7441 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     7501 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     7561 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     7621 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     7681 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     7741 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     7801 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7861 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7921 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7981 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     8041 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     8101 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     8161 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     8221 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     8281 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     8341 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     8401 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     8461 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     8521 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     8581 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     8641 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     8701 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     8761 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     8821 ggaccatcgc ttcataaata ctgcggtcac catgagggtg ataattcccc atgatatttc
     8881 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8941 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     9001 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     9061 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     9121 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     9181 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     9241 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     9301 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     9361 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     9421 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     9481 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     9541 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     9601 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     9661 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     9721 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     9781 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9841 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9901 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9961 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
    10021 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
    10081 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
    10141 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
    10201 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
    10261 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
    10321 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
    10381 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
    10441 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
    10501 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
    10561 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
    10621 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
    10681 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
    10741 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
    10801 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10861 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10921 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10981 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    11041 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    11101 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    11161 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    11221 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    11281 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    11341 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    11401 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    11461 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    11521 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    11581 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    11641 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    11701 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    11761 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    11821 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11881 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11941 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    12001 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    12061 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    12121 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    12181 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    12241 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    12301 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    12361 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    12421 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    12481 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    12541 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    12601 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    12661 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    12721 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    12781 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12841 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12901 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12961 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    13021 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    13081 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    13141 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    13201 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    13261 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    13321 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    13381 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    13441 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    13501 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    13561 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    13621 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    13681 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    13741 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    13801 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13861 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13921 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13981 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    14041 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    14101 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    14161 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    14221 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    14281 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    14341 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    14401 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    14461 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    14521 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    14581 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    14641 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    14701 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    14761 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    14821 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14881 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14941 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    15001 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    15061 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    15121 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    15181 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    15241 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    15301 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    15361 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    15421 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    15481 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    15541 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    15601 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    15661 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    15721 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    15781 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15841 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15901 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15961 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    16021 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    16081 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    16141 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    16201 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    16261 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    16321 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    16381 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    16441 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    16501 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    16561 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    16621 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    16681 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    16741 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    16801 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16861 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16921 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16981 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    17041 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    17101 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    17161 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    17221 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    17281 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    17341 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    17401 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    17461 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    17521 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    17581 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    17641 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    17701 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    17761 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    17821 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17881 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17941 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    18001 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    18061 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    18121 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    18181 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    18241 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    18301 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    18361 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    18421 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    18481 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    18541 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    18601 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    18661 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    18721 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    18781 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18841 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18901 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18961 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    19021 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    19081 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    19141 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    19201 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    19261 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    19321 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    19381 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    19441 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    19501 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    19561 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    19621 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    19681 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    19741 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    19801 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19861 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19921 gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata
    19981 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    20041 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    20101 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    20161 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    20221 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    20281 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    20341 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    20401 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    20461 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    20521 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    20581 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    20641 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    20701 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    20761 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    20821 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20881 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20941 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    21001 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    21061 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    21121 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    21181 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    21241 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    21301 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    21361 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    21421 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    21481 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    21541 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    21601 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    21661 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    21721 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    21781 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21841 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21901 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21961 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    22021 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    22081 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    22141 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    22201 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    22261 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    22321 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    22381 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    22441 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    22501 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    22561 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    22621 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    22681 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    22741 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    22801 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22861 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22921 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22981 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    23041 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    23101 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    23161 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    23221 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    23281 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    23341 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    23401 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    23461 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    23521 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    23581 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt
    23641 ttctttttca atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg
    23701 aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt
    23761 ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac
    23821 gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt
    23881 gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa
    23941 ttttctactg aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc
    24001 tctcatgatt tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaacaa
    24061 gtggtgcttt gatttctttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa
    24121 ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa
    24181 tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga
    24241 tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga
    24301 taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa
    24361 gcagtcttct cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt
    24421 catgacacat tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat
    24481 tttgaacact ttgattgcaa aagacgtttt tagattgccc gaaatgaatg caagattgtt
    24541 gtcgacctct gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc
    24601 tgcttatttt caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg
    24661 accgatctgg gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg
    24721 ttcaggaacg tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg
    24781 cttattttta tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat
    24841 tatgatccca gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga
    24901 tttcaaagta atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat
    24961 aatggttgtt tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt
    25021 ttgattttac gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct
    25081 caacagactg cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa
    25141 tagacaaatc ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta
    25201 tcttttgaaa ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat
    25261 tgtcccgtta gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat
    25321 ttcaggtttt tagttagata aggttcctta actactttcc cattgatctt caattgatca
    25381 ttttggtagc tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca
    25441 tctgctaatt gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca
    25501 accatatcac cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg
    25561 aataaaaatc cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca
    25621 agccagaagc gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt
    25681 tttttcattc tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa
    25741 taaaaataat tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa
    25801 aactatctct tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat
    25861 atccatggac ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct
    25921 agagctgtgc ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc
    25981 atcatgtgtg ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct
    26041 tcgttcctcc aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt
    26101 tcaggatgct ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct
    26161 ggttcgattt ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta
    26221 cttttttcag caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg
    26281 tcaccgcgat cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt
    26341 tctactaatt gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta
    26401 tgtccagtcg tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta
    26461 tatcctgaca tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag
    26521 aagcagcctc ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc
    26581 cgatgatttt ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt
    26641 atttgagttg tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc
    26701 atcatttact aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg
    26761 atccaaccta agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt
    26821 atagtttctc ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga
    26881 aagacttcta gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa
    26941 atagaaatcg ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc
    27001 caaataaaat
//
LOCUS       DACCWV010000023        26849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCWV010000023 DACCWV010000000
VERSION     DACCWV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.023"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            601..933
                     /locus_tag="IVW84_000881"
     CDS             601..933
                     /locus_tag="IVW84_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7917095.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1552..1893
                     /locus_tag="IVW84_000882"
     CDS             1552..1893
                     /locus_tag="IVW84_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7917096.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            2007..2246
                     /locus_tag="IVW84_000883"
     CDS             2007..2246
                     /locus_tag="IVW84_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917097.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2313..2522
                     /locus_tag="IVW84_000884"
     CDS             2313..2522
                     /locus_tag="IVW84_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917098.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            2582..3253
                     /locus_tag="IVW84_000885"
     CDS             2582..3253
                     /locus_tag="IVW84_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7917099.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3306..5282
                     /locus_tag="IVW84_000886"
     CDS             3306..5282
                     /locus_tag="IVW84_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7917100.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGANSKYIKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEIVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGKYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGVLSAVVYFKKKA"
     gene            5297..6049
                     /locus_tag="IVW84_000887"
     CDS             5297..6049
                     /locus_tag="IVW84_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7917101.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            6065..6826
                     /locus_tag="IVW84_000888"
     CDS             6065..6826
                     /locus_tag="IVW84_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917102.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6839..7099
                     /locus_tag="IVW84_000889"
     CDS             6839..7099
                     /locus_tag="IVW84_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917103.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAVSMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            7096..9186
                     /locus_tag="IVW84_000890"
     CDS             7096..9186
                     /locus_tag="IVW84_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7917104.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDFGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYTHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEVSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            9213..9677
                     /locus_tag="IVW84_000891"
     CDS             9213..9677
                     /locus_tag="IVW84_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917105.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLEIYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRMKEKLIHDGSQSNKYKLQKTSE"
     gene            9674..9916
                     /locus_tag="IVW84_000892"
     CDS             9674..9916
                     /locus_tag="IVW84_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917106.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            9934..10236
                     /locus_tag="IVW84_000893"
     CDS             9934..10236
                     /locus_tag="IVW84_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917107.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10307..12361
                     /locus_tag="IVW84_000894"
     CDS             10307..12361
                     /locus_tag="IVW84_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917108.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            12361..14973
                     /locus_tag="IVW84_000895"
     CDS             12361..14973
                     /locus_tag="IVW84_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7917109.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNEEISEMI
                     GKRLLLESEKVVKE"
     gene            14970..17501
                     /locus_tag="IVW84_000896"
     CDS             14970..17501
                     /locus_tag="IVW84_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917110.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            17551..17883
                     /locus_tag="IVW84_000897"
     CDS             17551..17883
                     /locus_tag="IVW84_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917111.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            17884..18477
                     /locus_tag="IVW84_000898"
     CDS             17884..18477
                     /locus_tag="IVW84_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917112.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            18640..20334
                     /locus_tag="IVW84_000899"
     CDS             18640..20334
                     /locus_tag="IVW84_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7917113.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21022..22899
                     /gene="ltrA"
                     /locus_tag="IVW84_000900"
     CDS             21022..22899
                     /gene="ltrA"
                     /locus_tag="IVW84_000900"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7917114.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIISYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23030..23407
                     /locus_tag="IVW84_000901"
                     /pseudo
     CDS             23030..23407
                     /locus_tag="IVW84_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            23407..24612
                     /locus_tag="IVW84_000902"
     CDS             23407..24612
                     /locus_tag="IVW84_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7917115.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            24628..25272
                     /locus_tag="IVW84_000903"
     CDS             24628..25272
                     /locus_tag="IVW84_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917116.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25283..26089
                     /locus_tag="IVW84_000904"
     CDS             25283..26089
                     /locus_tag="IVW84_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917117.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26111..26341
                     /locus_tag="IVW84_000905"
     CDS             26111..26341
                     /locus_tag="IVW84_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917118.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         9895 a         4168 c         5577 g         7209 t
ORIGIN      
        1 cctttttttt gagttataat aatacgcgaa gagagataat ttcattattt aaacaagcta
       61 aaaatcttga tattacttct tttcaggcaa tacgaatatt attcgcttac attttttgta
      121 taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttttgcac ttttggatga
      181 taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt
      241 aaccttcgtc agcgttcata cacggcatta agatttggcc cttaatgcgt taacctttca
      301 gtgttacggt attacccgca atggatttag tataacagat attcaaaagt gataccactt
      361 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
      421 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
      481 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
      541 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gaaattgggt ggtttatttt
      601 atgtcatata caggaatttt gagtctcaaa gatatttgtc actacggtaa acggtgtacc
      661 gctacagaga aaatcacgaa gaaactatca actggccaaa gtaaaaccgt tgtgcagtgt
      721 aagaaatata taatccagaa agacaaagtc tcagaggaaa tgatttatta cattgggaaa
      781 caaaagcaaa taattttaaa agatcctatc cccttaaaag agctatatcc aacaatcaaa
      841 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
      901 accgcaaaag ggatggggcg tttgatcggc tgaattgatc attctcataa cttgtatttt
      961 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gagtcactag
     1021 aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt
     1081 ttaacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt tggatagtgg
     1141 tgaaatgaat ttgtgtaaat aataagcgca gagtaaacaa gagggaattt agaagttaga
     1201 caagttagaa tagcgtctag attaatcatg agcagctact aagggatcta gactttgacg
     1261 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     1321 tcgtgtgctg cagtacatgt agatacttgc gaggacgttg attattggat caatcgagca
     1381 tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg aaatttttta
     1441 tgtattttta agtaaacgaa aaacgataaa atatgaacaa gtcctgaata aagtgaaaaa
     1501 attaatataa agcatagaga aaatgaaatt tacgtaatgg aggaatgaca aatgaaaaat
     1561 tatgatccga atatacgatt gggcacacat actattaagg tttctttcca acgttgggat
     1621 tacaaaggat ttgtgacgtt tagaaggggc ggaaattgta aaggattaga tgttttagca
     1681 ttagacgagg atgatttata cgatcaaaca ttgactgata atccaattgg attcggcttg
     1741 ctacctgaag atgatgaagg taatgaatgg tttaaaatga cgcttatgaa tgataatgga
     1801 gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt cagctttgaa
     1861 atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat tttacagata
     1921 aaggcttaga aggagtcacg acgattaata aaaaggtagt attaccttag gcattgagta
     1981 aggaatttat ttaggaggaa tagtagatgg cgcgaccagt tttagaggcg atggatatgg
     2041 atactttgac aaaaggaagg tatgtacagc tgttagctcg tggttattca cctaaagaag
     2101 tattcaaagc cttatctaaa ggaacagata tggaaaaaga aagattgcga aaagaattcg
     2161 attattggag aacccataat ggaatcaagg atttgaaacc agcacggcca gtaattaaac
     2221 gaaagaaaaa gataacccaa gactgaagtg tagagcgtat gtgatcatag gcttttttat
     2281 ttttgcaaaa aacaaggaag gtaggaaaac tcatgaatta tgaagaaatc gaaaacagaa
     2341 aaaaagtgtc gaaagaaatg gaagaaaagt tactaaaaac gatgaagcaa aaacacctta
     2401 aaagattatc tgtcgcgcaa tatatcaatg atatgcagtt aacaggaaaa gagaaagctt
     2461 gtttgttagg ttcaatgaaa aactttgaac agttgagacg aacttataga attcattttt
     2521 aaaacttttt ctatggaaat gagaaaaagt ttttttgttt tgtccgaaag gaggcagatg
     2581 gatgaaaaaa tggttatttg gttttttagg agtcacttta atcgtctttt gcagtgtgtt
     2641 tggctatgtt agctaccaaa aacatgaagg agaggtgttt aagcaaaata tagaaaaaaa
     2701 gatgcccgta gatcaaatca atgcacatgc aaagagttat aaggaggatg caacgaacgt
     2761 caataatgat atgtctttgg ggcaaatgtt gtccatacaa aaagaagcaa tcgaaatggg
     2821 cgtcaataaa caagtatttg cacaaattca gattcctgca cttggtttag cattgccaat
     2881 ttttaaaggg gcaaatcaat acactttgag tcttggagcg gctacttatt tttatgaaga
     2941 tgccgaaatg ggcaaaggaa actatgtcct agcaggtcat aacatggaaa tgccaggtgt
     3001 tttattttca gatattcaaa agttgtcatt aggggaggtg atggacttag ttagtaacga
     3061 tggcgtctat cgttacaagg taacgtgtaa atttatcgta tctgagtact ttaaattaat
     3121 tgatggcgtt cctgaagaga actctttctt gtctttacca aagaaaggag agaaaccctt
     3181 attaacgttg tttacttgtg tttatacgtc acaaggaaaa gaaagatacg tggtacaggg
     3241 agagctacaa taacaaggtt gtaatcaacg cgagtcgttg aaatatgaaa tgaggagagt
     3301 aaaaaatggc aataaatcgg tatgtaaaaa aagctggaat gcttgttgga atgttaggaa
     3361 tcattgcagg ctgctttcag ggatatcgag catatgcaga agatgtcacg caaaaaacgc
     3421 caccagagaa ggtcaatatc acggttcata aattaatgta cgatcaagga acgcaattga
     3481 atgttgatat tgacgggatc aaaaacgatg gttatacgca cgatcaatat cctacaggtg
     3541 taacgaaata caacaaggca gattatggtg atgttgagtt tacgttagga aatattacgg
     3601 atcaagtatt accgactgaa gattctgatc taaccaacgc gaaggtggac gaaatcgtta
     3661 aagatgtaga agacaaaggt gctaatagca aatacattaa aaatgcaacc aacaaaatta
     3721 cttcagctgt agatgaaaat ggtgagatca cttttgcgga tcaaccagcc tatgtcaatt
     3781 ccaaagggaa tgtatatgtg gtttatgaaa gtaaatccgc tgctggatta gtcacacaaa
     3841 aagcaaaacc aatggttgtg attgcgccaa tgacagacaa tacaggatca ggatttttaa
     3901 aagatattca tttgtatcca aagaacattg tttctaagtt gagttttgaa ttaacgaaat
     3961 ttggtgacga tggcacagct caaagcaaac aaacaccttt aaaaggggca aaatttgagt
     4021 tatacaaggg cgaacctgga aaaggaacaa aattaggaga tcttgtatcg gatgatcaag
     4081 gaaagctgac tgcgactgat ttgacgttag gaaaatacta ttttgtggaa gttccttcag
     4141 aaattgtagt gggatcagat aaagaaccaa cagccgatca atatttatta ggcgcagacg
     4201 ctcgcaacga tgcgcacaat aaattaacct ttgaaattac gaatgacggt gtgacaagcg
     4261 atttaaaagc ttcatacgta aactataaag caccagtgat cgataaaaca gtaacaaatg
     4321 gaacaggaca agaacattct ttccaaattg gtgacgcagt aaattatcaa ggaacgattc
     4381 atatcccaac agacattgca ggcggagcag acggtatcac agtaaacggc gtgaaaagcg
     4441 aaacttctcc ttacagtgta tttaagtggg gggatacagc aggacaagga ctttcttatg
     4501 tcgctgctaa agcaaatatc aaagtaacaa ataaagatgg atctgtggta ttaaaagaaa
     4561 atacagatta taaaatccaa aattcagaaa atggctttgt gatcgatttt atcgtgaata
     4621 atggccaagt aagtgatact gtagcaagtt tacacggaca agatcttcaa atgacttata
     4681 acatgtatgt aaatgatagc gcagctgttg ccaatccatt aacaaatagc gtggatttcg
     4741 tttataacaa caatccattt aatcaggaag aacaccacga aaaaacgaaa gctgacgttg
     4801 tgacttatgg tgcaaaattc ttaaaagtcg atagcggact atttggaata ggaatcaaag
     4861 caacaccgct tgaaggcgca gaatttgctg ctaaaaacgc agaaggaaaa tattatggtg
     4921 gattagttga tacggataaa gacggtgtca aagaagcagt atgggtagac gatgtagcaa
     4981 atgcagcgat cctaaaatca gataaagaag gtcactttga aatcactggt ttaactgaag
     5041 gtaaatattc actggaagaa acaaaagcgc ctgaaaacta tcaaaaatta acgaaagaga
     5101 tctctttcaa agtagacaaa gattcataca aagaagaaaa ccgaatcacg atcaaaaaca
     5161 atcaaaaagc ttccgtacca atgaccggta gcaacggttt tcaaacgtat gtattaatct
     5221 cttgcttgtt gctaggagca ggggtacttt ctgcagttgt ttactttaag aagaaagcct
     5281 agaaggatca aaaccaatga ttatcaggaa gcctaagaaa aagcgaatca tgggtaaatg
     5341 gatcattgct ttctggttac tttcagctgt aggtgtctta cttttgatgc ctgctgaagc
     5401 gagcgtggcg aaataccaac aaaatcagca gattgccgca atcgatcgaa caggaacggc
     5461 agcagaaacg gattcttctt tggatgttgc taagattgag ttaggcgatc ctgttggtat
     5521 tcttacaatt ccaagtattt ctttaaaact accaatttat gacggaacgt ccgacaaaat
     5581 tttagaaaat ggtgtaggta ttacggaagg aactggtgat attacaggag gaaacggtaa
     5641 aaatccttta atcgcaggtc atagtggact ttataaagat aatctttttg atgatttacc
     5701 ttcagtaaaa aagggagaaa agttttatat caaagttgat ggtgagcagc acgcttatca
     5761 aatcgatcgg attgaggaag tgcaaaaaga cgaactacaa agaaactttg tcacttactt
     5821 agaaccaaat cccaatgaag atcgagtaac gctaatgaca tgtacgccta aggggatcaa
     5881 cacacaccgt tttctagtgt atgggaagcg agtaaccttt acaaaatcag aattaaaaga
     5941 tgaagaaaat aagaaacaaa aattatcttg gaaatggctt ttaggaagta ctgtttttct
     6001 ttcagtcatg atcattggtt cgctatttgt ttataaaaag aaaaaataaa ggagggtaac
     6061 caacatgaga aatagaaaat ggctatttgt ttgtgcagcc gctctttttt ctttcactgc
     6121 ctacacacag ttgacgtttg cagacagaca agaaagcacg gatagcacag aacaaacaat
     6181 ccaaacgtca gatagcagca cgattcaaag cagcgatgaa accgtcgatt cgtcaacagt
     6241 tgattcttca gaagaaccta aaattgctga agacgatcaa atagtcaaac agaccattca
     6301 cgttcaaaaa attatttctt ccgaacaatt agacaaccag gggcaaaagc ttgaaaaaca
     6361 agaaggagta aatggcgcga agttttctgt ttatgatgta tcggacattt tacaaaagat
     6421 ggatgttaag gatctcacta ctgaccaaat tgaatcccaa ttaaaagatc gagtgaaaaa
     6481 actatccagt gatcaactga agctaatttc taatggagaa acaaagacaa ttgatcaaca
     6541 aacaggtgtc tttgaattct cggtagaagt acaggcaaat cagaaacaag cttattatat
     6601 tgtcaatgaa tcaagtcctg aaaacatttc taatagcgag gatattttgc tattaacacc
     6661 tgtttctgat aaaaatggtg aattcttaaa ggatgtgtgg atttatccta aatcagaagc
     6721 ttcgcaacct aaagaagaag taaaaaaaat cgtatctact ggcgtaaaga aaaacttctt
     6781 tgagaattgt tgggattttg ttactcactt gttttctaga gattaaggaa gaaataagat
     6841 gaaactgatc aaattattat tattcaatct ttcttgttta accgcagtct ttttgtactg
     6901 cggtttatta ttttctcaac tatctatttt gccaagctac caactatacc taatgaggat
     6961 tttaaacata ccagctgttt ccatgccgct agatattatg gaaaagctca ttaggaatcc
     7021 attatttact acgatcaatt tgatcgttat tgtcggaatt tcactagtca tttataaaaa
     7081 agaggggatg aagggatgaa ttttaaacac tcgtggacaa gacggttaaa atatgttttt
     7141 cccataatga tgatgttagg tacattgttt gggttaaaaa cagccaatgt ctttgctgaa
     7201 aaagttattg tcgatccgtc aaatccaata cagaacgtaa agaataattt tattattcca
     7261 aaagatgttc ctaatgcaaa aacaaatatt actgtaaacg gagcgtcaaa agtttacaaa
     7321 tatcatgttg atgccaacaa aggtggtttt acagacgact acgtaggatt agtggaggga
     7381 aacaaaaata tacgctatcc ttctttccaa aaggaatacg gtgaaacaaa ccttgtttta
     7441 gaaggcgtaa caaccaacac caaagtcaat attgactttg gaaaaatcgg tacgtataac
     7501 gggaaaaaag tcaatatcaa gctagtttta tctaatattc acttatactc ggatacgttg
     7561 ccgtggaatc tactagataa taactataca aaaactcatt ttcgagacga tggctataaa
     7621 aatacgaatg gtgcaatgag taaatctaaa aaaagaacag tactttggat ctcagacaat
     7681 cttttctcag gaattgttta ccattcaaca caaatgaatg tgcagctagt agccacatat
     7741 gaggacggta gccccgttca attttcagga gatacattta tcagttttaa ctctttgaac
     7801 cctgcaggag gaaaaagcac ggatcttaaa ggagaatata cccactatga caaaatgaat
     7861 acaacagatt ggtatgtcag aaaagatacg gttctttcag aatttaaatc tttctacaac
     7921 aatctgaacg tggtaggtgg ccacccagga ggaagctcta agctgacaca agcggataat
     7981 gatttcaata atttgcatga taaattagga gatccgaagt ttggtcaagg tactgtgtcc
     8041 tttaaaatat ctgaagcaaa tccgacgttt gtgattggtt cttctaatgt tcaaacctgg
     8101 tttactttat cttctgcgac aatctttagt gttgtacctg atcaacctga aaaaacaggg
     8161 gttgataaaa atggaaataa cgtgaacgat aacatgttgc aagtaggaga cacgattcaa
     8221 tatcgaatca aacagaaggt gaatcgatta ggagtggatt tactagcggt ttacgatcga
     8281 tttgagttaa tcgataattt accaaaagaa gtagattacg tcgatgcaca cgttgaaagt
     8341 ggtacaaata aaaaattcga tgtatctggt gaagtgacct atgacaaaac aaaacaccag
     8401 gtaaaatatg cagcgaaagc tgatacatta aaaaatcgaa tgaagtataa cggcgaaact
     8461 tacgaattag tgatcaatgt aaaagtcaat gaattggcca atcaaaatag tgtagccaaa
     8521 aatcagggaa cgtcgatcat taataaagta gaaaaggata caaatatcat taccgtttat
     8581 ttccctaaaa ttccagtgaa agaagtacaa caaaatggaa aagatgtaaa cggacgaaat
     8641 gacggtaaga agggtacgcc gacagctcct ttaaacgcag gatctgaagt acaatatcta
     8701 gtcacacaaa aatggcacac aaaaggcgta gacgctgcgt cagatcacta caaacaattt
     8761 agtattcaag atccaattga agctcgtttg acatataaag aagtttctgc gcaagtaata
     8821 gataagagta aaggaaaaga tattacttct gaaggcacac taacctatga tagcaattca
     8881 agaacgctga agtgggaagc ttccgctgac ttcttaagta acaatctttt agatggacga
     8941 gaaattcagc tgatatttac agctaaaact ccattacaat cagaaaaaaa tattgataac
     9001 caagccgtag tagcagtaga taatgtttct aataaaacaa atgttgtgac aattggggtt
     9061 gatcctaact tgccacaggt cattgttcct aaaacaggtt ctacacattt agtaacgatt
     9121 tcagcggtca gcttactatt acttgtgtta gcaactttta gttatgcggt tctgagatac
     9181 atttaaaata aaaaaacgaa agaatgaata aaatgaaaaa gttaataata gtagttactt
     9241 ccttgattgc catgattgtt attttagtcg gatgtagtaa tgaaaaaaat aaacaaacta
     9301 atcaagataa tggcgtattg aaatcaggaa ctatgtggaa agaagaagta ggtggtcttg
     9361 tttataattt gaaaattata gacgagacta cctgggaata ctctgagagt gtatggcatc
     9421 cagatcctgt acaaataaca gtcaagaggc aaaaagacta taaaggttta gaaatataca
     9481 aaatagtaga ttcagctggt gttagggaat ttataaacaa atctgattct ttatttattg
     9541 ttgttcccta tgagaagaat ggcgtaaaaa aaataatttt tcttggatct agcaaggatg
     9601 aaaagcgaat gaaagagaaa ttgattcacg atggatcaca atccaataaa tataagttac
     9661 aaaagactag cgagtgattg ctagtctttt tatttgtcct gaaggaggaa tcacagtggc
     9721 aaaagctaaa acgccatttg aaaaagccgc gtctaaaatc ttagaacgcc gcggtgtatc
     9781 tgaaacacag tggaaacaag aagtccttga aaaagcacaa tttgacctat tacggggaaa
     9841 agacaaggac ttagaaggat atatctatca acgtgaacgt gaaaagcttg ttttcgatga
     9901 aattattaaa cactaaaagg agcgaacaat tttatgtttg aatcaattac aatgaatttt
     9961 ttaacacaac cgaaagtagt agctgcggtt gatattttta gagcattaca aactgccgca
    10021 actagtttga aaaataatct gatgattttt gctgctgttg cggcagtggc ttccattgct
    10081 cttggaggta ttttcttctt ctttgggcga agaggagctg aaaacgggaa gacacacgta
    10141 ggttatgtat tgttgggggt aggagttatt ttagctgccg cttctattgt agtttcaatg
    10201 ttctcaatgt ttggacaaac cccacaagga ctataaaaaa acaagtagaa ggagtaaaaa
    10261 gtacgtggct actttttact ccttttttta tggaaggaga atattcatgt tttttacgat
    10321 ttcaggcaat gcaaaattgg ataaagagtt aataagaaaa gcaacgaaga aagtgggaaa
    10381 gcgtacttcc tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct
    10441 acatgtgtta ggacaagtgt tagaacaaga gaatcaatct tctgttaaat tgactctcac
    10501 gttacatgat aaagaaggaa aaatagaaaa ggctggcaca gaatacgttt ttggctcact
    10561 ggattatcaa atttcagaag gtttttatcc tacattgatt gatttaatcc gcctagatat
    10621 atttaaagat acggatgaaa ccatgagtgt ggaagagtct tatcgaaacg aaaaaatttt
    10681 acaagtttta gaagaagagt tagaaagcga ccaaagaaaa gtgaatcaag tgagtgaagt
    10741 cgaagcaaac gaagaagtac catgggaacg cccaatcttt ccaaaggatg aatcaaaaga
    10801 aaaagagaaa ggaattattg acgaacaagc tgacattttg actcataaat caatatttga
    10861 ggaagagaac gaggtaatgg gtcaattgga ggtagatccc tttgaagaaa caatggagga
    10921 tgaagagacg gccgaagtag aagttgatcc agttgaagct aatgaagacc caacatggac
    10981 tgaaggggat tctcaggaat atattgaacc acttatcagt gaagaagtag agaaaaatga
    11041 aacagagata caaaaggcaa cggtatttga ttatattttg gatcaatatc cgccagagca
    11101 tgaatggatg aaagaaaaaa ataaattatt tttaaatgaa ctttattcga aatatcaatt
    11161 acctcaaacc tatcaagcat ttttagagag taaagaagaa ttaatctcac agggtaaagg
    11221 cacactttta gaacagttag agtctgtcca atcaacaaat tggcatgagc ttgcacaaga
    11281 agatttatta gctacttttg aagaacgtca acaagaaaca gatcaagaaa ttaatgagta
    11341 tgcgacggaa cagtcgtcga attgggagaa agcaaaagag gaactaaata aagaagaaca
    11401 gcgagtgatt gaagaagaag tacaaaaaat taaagagcgg tacgaaaaga aacgtgacaa
    11461 tgcgtattcc ttgtgtgtag aaaagatcaa caattttact cagacgaatc aagaagcatt
    11521 atcgaaagaa aaagaaaact tattaaaaga acgtataaaa gaacgaaaag aagaatttta
    11581 ccgagggttg cgtactgata agctaaggat tagcaaagaa ttaaatgaac aactgaataa
    11641 actctatcaa actacgactg aagttctgat ggaaaaacac gagcagatcc aacaagaatt
    11701 acaagcacaa attccaaaat ggaaaaaaga gtatgaaatt caccaacaag aattgaaaga
    11761 acaagaagaa aaacaacgga aaaaagaaaa agagcgcgaa caattagaac taaaaaaacg
    11821 tgaacaggat cttgcggaac aaaaaatggc gatagaaaaa caacggctag aacaagaaaa
    11881 ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa atggatacat tgcgacaagc
    11941 acagttaaat tatctgacac atcctcaacc agtagctgct gttcaaacgc cagtgaacaa
    12001 ccaagcaaat ttaactcctt caccccagcg ttcctttaac ggatggatgg tgggatgtat
    12061 tgcaagttta agcttgcttc taggtggagg aacagtattt gcttttaatc atatcaatca
    12121 atcttcacag gctgattctg tagccacaac ggcgtcaatc aaggaagaag cccaggaaca
    12181 agcaagaaaa gagtatgaac aacaattaga agaattattg aatcgtacca atcagtcaac
    12241 aacagagtca agcatggaaa atagcaaaga gagtgattct agtaaggcta aagaaggatc
    12301 aagcgattca actgtacaat caaacacttc cgaaagttca gaatcaaatg aggtgaaata
    12361 atgcgtgtta gagaaacaac gaagcgaaaa gacaaacgat tcaatttaga aaagaatagg
    12421 tatctttctc atttctatcg ctatactccc tcaggagaag agctaccaga aaaacatgac
    12481 aaacgcattt cgaaaaagat tttttatagt ggacttgtat acctcccttt agagtattta
    12541 gtaacaagag taccagaagt tttcaaccga ttggaaattg ttaagggaag cttacaagga
    12601 ataaatctgt ttcaattaca ggaaatgcca caggtctttc attttgattc agctggtttt
    12661 tggaatccat ttactcctac aactagtttg ggtacacgtt tagcaatttc gacagtttgt
    12721 gtggttccag ctgtacttat gggattgaaa atggacatgt ggtttcgtcc tctagcagat
    12781 ggacaaaaag gggataatcg attaatgacc ttaaaagaaa tccaacagat ttatccgcaa
    12841 atacctgaaa caaaaactac ttttccaggt tatggaggta ttccaatcac acattacaaa
    12901 aaatattggt acattcaaac agatactgtt aatacgtgta ttgttggtac ctctcgatca
    12961 gggaaaggtc agattgaagt gttagccacc attgacaatt tatctcgtgc agaaaaacaa
    13021 agttcaatgg tcgttaatga tccaaaaaca gaattatatg ttggtagtaa agatgaacta
    13081 gaagctagag ggtatgatgt atatgccttt aatatcaccg atccactgca atcaatgagt
    13141 gataatccat tggcactcat cgtgaaatac tggaaacgag gagacataga tactgcaaca
    13201 cagttaacca atactttcac aaatacgatt tattatgatg ccaacgcttc agataacaaa
    13261 tactttaacg aaaacgcgca gaaagctgtc aatgcgctta tttttgcgtt gttagaacaa
    13321 gctgatcaaa caggagatta ctccaaattg acaccgaata atctcgttga gcttctttct
    13381 gagattggcg gttttaatta ttatccagat ccagacaatg aattcaaaca gttgaatgcc
    13441 ttagatgaat ttatgaatca gttgccacct ggaaacattg caaaaaaaca atatggggca
    13501 acgaaaattg gatctgataa agcgaaggga aatattttat caacggcaat cacgggattg
    13561 aatccattta ctctaacgaa aattgcaaag atgacttctc aatcaacgat tgattataaa
    13621 tcggttgggt ttccaaaata cttagatttg aaattacatg aaagtctttt aaatcaacga
    13681 gtcgtagtag aattttatca taagggaaag aaatttcatg aagaacgtgt caaagtaggc
    13741 tataaaggat ttagtgaaat caattttgat tgtcatttag ttgcaggtga tcaagtttgt
    13801 ttaaaatacg atcatcaagg aaaagaatat gtggcttggt atgaattttc tcaaagagta
    13861 ttgaaagacg aagagggtaa tatcgtttat cggaaaaaac gaggagaaac ccatttgcca
    13921 gaattagaaa aagaagccgt tctaacatta gatgaggaaa aaagtaactt attagtagag
    13981 tctattcgga tgcactatac cgataaacca atggctattt ttatgcttac acctgattat
    14041 gatccatcta accatatcat tgttagtatt tttcttagtc agttatataa agaattatct
    14101 acgcaatgtg ttaagactaa aggagataaa tgtcaccgca ggattcattt tattttagat
    14161 gaatttggga atatgccacc aatggataat atggaaggaa tcatgacggt cacagctggc
    14221 cgaaacatgt tgtgggatct gtttattcaa tcttatcagc aactttatgc gaagtatgga
    14281 gaacaaaacg cagcgattat caaagaaaat tgtcagacac atatttatat tatgtcgaca
    14341 gatgataata ccatcgaaga attttctaaa aaagtgggga ataagacggt tgaacaagag
    14401 aacagtacca tcaattcatt aggtatgaat actcatatca atcgcaatgt tgattcagat
    14461 cgtatcttaa ctcctgaaag aatttctact tttttagaag gtgaaacgat tgtactcgct
    14521 cctttaaaac gacaagattt gaaaggtcat aaaatacggc cgtatcctat ttttaatcat
    14581 ggggaaacga atcttcctta tgcttatcaa tatattgaag atttcaatcc atcgtgggat
    14641 atcaatgata tagacataca ttgtacacat gcggacttgg atcttaaggc tttacaaatt
    14701 gattatgatt atttcctacc agatgaatat gctagaaatc tctatcatgg actgcatgat
    14761 caattagaag aaaaaaacga agaaaatatg ttttataaaa tcgttcaatc tcttgagaat
    14821 gagacggcta aaaaattgtt aattcaattt tacgaagaac aagcagcaga tcgacttcaa
    14881 gaatatgttg aaaagttgat caaaaatgaa gaaatttctg aaatgattgg gaaacggttg
    14941 ttattggaat ctgaaaaggt ggtgaaagaa tgatcattgg aggaattttt tctggcatag
    15001 ccgactggtg gtcaggctta ttcggagaag gggatgcaaa ccccaaaacc gtaacgaaag
    15061 tcttacaaga ttatggtaat tatctgcagt atcaagatgt gttcagttgg atttgtaata
    15121 cttttttatg ggggttgatc aaagggttat ataatctcaa tactttacta gaaaaaacca
    15181 tttatcaaag ctttgattta aaagacttat tgaacgccgc tggggtaaat gaactatttc
    15241 aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt cacattaatt tatttaggct
    15301 tgaaattttt tatgagtaaa cacccaccaa aagtcaaaaa tatcttggtt aatttattta
    15361 tggccatgat cttgattttt ggtggtagtt ctttaatcga tgaagggtta aatatcagta
    15421 aaagctttta cggagacgtc actgcagcaa ataaagcaga taaaacgggt tcacctgctt
    15481 ttcaaattat tcaaaataat gtgatcgatg tcagtacatt actagcaact gacccgaata
    15541 aagtagaatc cctgccaaca gacaaaagaa atgctttaac agcgaaaaat tttaaaacag
    15601 cgaatatcaa tgggattatt actcctaaac aagctaaaga aatggcggat aaagataaga
    15661 gtggtttatc agatttacaa aaggatcggt taagagcgtt acaataccga ctggaactgg
    15721 atgataatgg aaaagaaatt cctgtgcaaa tcagtgatga aggcttagcc aaatatgttt
    15781 atacttcagg gtatcgtcga tacattagta gtccagggat tattttagct ggtgaatctt
    15841 ctttggcggt ggcctattta tttatcctat ttacgatcgt tacttgcatt attgagctgg
    15901 tatttaaaaa attctattta gtgattgccg cgtcaacgga ctttgagaca ggacaacgga
    15961 tgaaaagtgc tatcgaagat atttctcaat ccttcttgtt attggcattt acaattttag
    16021 aattacgaat ctatacctta atgctttcag gtattggtga tttgcatgcg gcaggaaaga
    16081 tcaatggatt cttgtatgtt gtcgctttga ttgtactaac cattgcgcta ttcaaaggct
    16141 ctcaatcagt tacgaaaatt ttcggcgttg atacgagtct gaaaaacggc agtaattctc
    16201 ttcttagcat gtttgcctta ggaaatattg cgaaaaatgt gggtagcgga gctaaaaacc
    16261 ttgcaagtgc tggtaagagt ggtttaagcc atttaaatga tattcgtaaa aatggatttg
    16321 gccatcgaaa agatgttacc cctgataacg gggaagaaca atcagacgat aaagctacaa
    16381 ctgtacctaa tagtaagaaa acagtttccg aaatgttcgg gaaagcgaaa aatggtgttt
    16441 ctaaagccgc agaaggtctt ggttacgtca atgaacgtgg gttaaaagga acagctgaag
    16501 atcttacaga aaaagccaaa gacaccttag aagataaatt agacggcacg agagctaaag
    16561 aattggctga agctgtgaag aatccatccg aagcactaga aaaacaaaaa gaaaaagctt
    16621 cagaagtcaa agaaggacta aaagatgcgt atggtgaagg acaagtcaat gcagcagtga
    16681 aagctaatac aacaaccgaa aaagaaaatc aagaaggtat agtgattcct tcagtcaaag
    16741 atcaccaaga agcgttgcac tctaatgaaa attcgattga agagaaaaat tccatagaag
    16801 aaagaaattc agaagaagga aaaaatggac agttaccaaa aactgatcca gaacaatatc
    16861 gctataaacc taacagtgac cttgaaaatg agaagcgaat ttcagattta tcaaaagtaa
    16921 atcaagaaga caaagcagaa aatgagggac agttaccaaa aactgatcca gaacaatatc
    16981 ggtatagatc tgttaactcc gaaaatgaaa aacgcatttc agatttacca aaacgaaatc
    17041 aagaagtaca aacggatgag gattccatcg ttctgccaaa tgataatgga atcgaagttg
    17101 gaactgaaaa atcaacgaaa attggtttac ctaaggcaca aaatttacca atgaaagata
    17161 gtcgtttgaa tcaattcaag gataatgatc aaagttttgt tcatcaatca cctgcaggta
    17221 ctgttcaaat gaaaccaagt cgtttgcctg aaaatggaaa tctgacaact gtttcttaca
    17281 atttatctga aagtggtgag acagggaatg cgtccgttca aaatgttgtg acaagagaag
    17341 ctgcaccaaa acctattcaa aacgttcata caggttacaa gccagcaaat gtggcattta
    17401 ctgcaccaac aaaaataaat gtgacaagag aaactaaaaa caggaacaat gcattgaaag
    17461 aacaactatt gagggatatc aaacaggata tcaaaccata aaaggcctac aagttaggcc
    17521 ttttctttat atgaaaagga gaaatcaatt atggataaat atataaacat gaaaaaccct
    17581 ccaagattta gtggagaatg gaagtttttc gcttttttct ttttagatct attaacgctt
    17641 ggtggctgct ttttttgggc gaaaatggtc aataatcttg tccacttacc actctttttt
    17701 gcactcttga attatggctt atggattgcg atagcgttag tgattgtttg gcgaccctat
    17761 aataacccag gtcaacgaca aatttttgcg attatcgagt ctattttttt tgcggacaag
    17821 aaccattatc atgcgttaga tcctaaccga aattatcatg gtacgtataa ggaggatgaa
    17881 taaatgcgag tcaaaggaca aaattcagtt gaaaggaaat ccattttgca gtttggaaaa
    17941 ccagaagagc gctctatctt ggacttattt ccgtataaat cctgtacaga agagggagtc
    18001 ttaattacta gagaagatca ttttcaacgt tattatcgtg tagtttctac agacgtggaa
    18061 ggattaaatg aacaagagaa actagaacga atgaatcagt tgaccaccgt catgcgtacg
    18121 tatgtgccaa caattaagtt agttagtttg accacagaga cggatctaac ggaacaaatc
    18181 gtgcaaaagc ggcaattatt agataaaaat cgtatggaac agtccttagg aagaaatcta
    18241 caacgattaa aaaaatatga aaagaaatta gtcgaagaaa tccaggaatt gaaacgagca
    18301 gaacaagaac gtcctgactt aagcttcttt ttcgtcattg aaggaaagac aatcaaggat
    18361 ttacaaacca gaaaccgtca gttaatgcga tctagtggca ttttagggtt aaaaccactc
    18421 tacaaaaaag aattgatcaa gattctgtat cgaatgaaca atatgaatga tgagtaggaa
    18481 tggagaagag agaatgaaat atccgttgat atggttagaa aaagatgttc cgaaggaaga
    18541 gaccactaga aaatttcctc acactttaag cctgggtaag ttgggaaaag ggcaatcttt
    18601 taaccaagta aagaatcaaa caagaagagg tgactgtaca tgttagaagc aattaaaaca
    18661 gctttttcac cacaaaatga tcaaaaagta cttgatccat taaagcaact aggaaatcgg
    18721 gaaatagaaa ttttaaaatc caaagggtat gatttagatt ttttaaaaca agtgatgcca
    18781 caaggtgggt taaattttga tgaagaatat gtgacgtatg gtaatggtta ttcacgatgt
    18841 gtgacagttt atcactacac aaaaaatcct acgctttttt ggttggccaa tctcatgaac
    18901 aatcctcata cagtggccac actcgatgtt agcacagatg aaaagcaaaa agtgattgat
    18961 caaatcaatc gtgccttaga tgaattaaat gatcgatcag ataacgaacg aaaagcgact
    19021 gaccgaaata aaagcgcaga agaatacctg gaactagcgg aatatgctcg taaattgact
    19081 tctaatgggg aaatttctaa gcaagtcaaa gtgagagtac atgtttacgg aaacacgatt
    19141 caagaagtag atgaacgagc gcaggaactg atcgaaaatc tcaaagggat ggattatcgt
    19201 gcggtgactt atctgcataa aaccaaacga gattggcaat cgatgtttac catgttcgat
    19261 gaacaagctg aatggttcgg aaatatcaaa gggcaatcac taccatcgtt aaacattggc
    19321 ggaggtttcc cgttccacca ccaaacgcta aaagatccag gagggacaca atatggggaa
    19381 actatgacag gcggcccatt tgtctggaat gcgacgtata aaaatagtgt acggttgtct
    19441 accaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct taaaatgatc
    19501 gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg aaaagacttt
    19561 attcctttct taaaggaata taacggtatt atggtgcgtt tagacggatc tgacggaatg
    19621 atcaatcctt tagagatatt tgccacacgg acgtatgatg aagcatccag tttatatgac
    19681 gatggatcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac acacttagac
    19741 aaagtggtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat gaaagcagaa
    19801 tttaagacct acttaaatcg tttttatgaa gagtatggaa cagtaccacg cggctttact
    19861 tcctcaaatt cgcgttcaaa tactgatacg caagtcacag ggaaagatcc agaagcttat
    19921 ccgacgttca aagaattttt agacttttta ggacaactgg aattaccagg tgcttcccaa
    19981 gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag aatccttatg tgagacgtat
    20041 ggcatgattt ttgatggtca tagtacgatt cgacaccttg accaacaaca gttggtcttt
    20101 tttgacattg actctattac aggcttagat cgaggggtac aacaatgtca aatgtacatg
    20161 gctatgacgt tgatttggaa tcaggcattg attcaaggaa gaagacaacg acgtttaatt
    20221 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
    20281 cataatggtg cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt ctaaaaacta
    20341 ttgtaataac tcttataacg agaagtcaaa tgagtgggta acgccacaaa gggcttccac
    20401 tgatactccg acatgcaaca agtagtttat gcagaccaac gttgtgtgaa gctcggtgaa
    20461 gtcggctgaa ggataccgta aggtaaaaga aatagtgaat accacgaaag ttctttagag
    20521 gtaaagggtg tatccgataa gtcgggtgtc caaaaaaact taatatggtg agtatgtaca
    20581 aacgtactga cgaactagcg aatgtactgt ccgaaaggca tagctcctag aaatgggaga
    20641 gacagtgagc aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag tcgttgttat
    20701 gaaagaccat gctgcgttac aggcgtagga gaagttaggg ggcaaaagct agcatctaag
    20761 ttaaggcatg tggatagata taagggaaca ggggaagcca tcaacatgga gagtgcatac
    20821 tcgatgaagt ggtagtaaga aatatgtgaa tgacaaataa cttactttag tgatggtgaa
    20881 agtagagtca cgactgatga aggttctgta atggaactgg aggaacaggc tcgattcgtc
    20941 tgtaaaagtc atgaaacccg ttcaaccatg ggattctcgt atgactaaaa aggtcacgag
    21001 ccaattggag cgtgataaac catgacaaaa gaaacaagtc caaaagatag gaagttgcgg
    21061 catgcagaat attatggcat gacggaaatc ttcgataaga tatactccaa aagtttgaag
    21121 ggagataatt tcaagaatct aatgcagatt atcatttctg ataacaacat tgtcctagca
    21181 tatcgtaata tcaagagaaa tcacggtagt gtgacaagag gagaagatgg agtaacgatt
    21241 aaagatattg aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa gcgattcgca
    21301 cattataacc ctcggaaagt aaggcgtgta gaaattccta aatataatgg taaattaaga
    21361 ccgttgggta ttccatcaat atgggataga atcgcgcagc aatgcatctt acaagtatta
    21421 gaaccaatat gtgaagcaaa attcaataat cacagctatg gtttccgccc aaatagatca
    21481 gcagaacacg caatagcaga ttgtatggcc cgaatgaatc aaagtcacat ggaatatgtg
    21541 gtagatgtag atatcaaagg tttcttcgac gaagtcaatc atactaaact catgagacaa
    21601 atctggtcta tgggtatacg agacaaacag cttctcttaa tcattaggaa aatgttaaga
    21661 gcacccgtag tacttccaaa tgggaagttg atatacccaa caaaaggaac accacaaggc
    21721 ggaatactta gcccattact tgcgaacatc aatctcaacg aatttgactg gtggatagca
    21781 aatcagtggg aggaaaaaga tgccaaagag ctaaaacatc attttaggaa aaatggagct
    21841 tgggataaag gtaatatcta tcacaagctc agaaagagta caactctaaa agaaatatac
    21901 ctagtgagat acgcagatga ttttaaaatt ttctgtagaa attataatga agcgaaaaaa
    21961 gttttctatg caactaaaat gtggttacag gagcgtctaa agttaccaat atctgaagaa
    22021 aagtcaaaaa tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt tacgatgaaa
    22081 ttagtgacca aaggtaacaa aaaggttgtt aatacgcata taggtacaaa agcacaaagc
    22141 atgattaaag tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc taatagcaaa
    22201 gataccttta tctatattgg agtctataac agtatggtta ttgggattca taattacttc
    22261 aaaattgcga ctcattgtag tgtagcaatg cgaaaaattg caagagaact cagaccaagt
    22321 tttgaaatac gactacgaga acaaggaatc tcaaaagaag gacaatacgg aaagtacgaa
    22381 ggatacaagc cttacctacg gtcaggacaa atacgatata taagagggtt tccaatatta
    22441 ccagtagggt atattcaaca cagacatccg atgtgtaaaa aaagaaatat taataaatac
    22501 accgaagaag gtagggaagc cattcataag aagcaacaaa ccgtagaaga atggaagata
    22561 tcgtggttgt gtagcaatcc agtgcttagt aagagagcaa cgattgaatt tgcggacaat
    22621 cgaatttcaa aattcatcgc tcaaaaggga agatgtgcag taacaggtga ggagcttatc
    22681 ttatctgaaa tgcattgtca tcatattatc tcttatcatg aaagcaaaag tgatagctat
    22741 gaaaacctag tgattgttac tgaggaggtt catcgggtaa tacacgctac acaaagtgaa
    22801 acgattgagg aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga aaaactcaat
    22861 gagttgagat taaaagttgg taacgaagaa atatcttaat aaatatttgt aggacgggaa
    22921 aacagaatgt cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc acgtacggtg
    22981 tggactgggg gaaaagacgg agataatatc aaactcttac ctatcagtat cgattaatcc
    23041 agattttatt gaaacggtca attatgtcac aaactttcaa cgagaaatgc gtaaagtaga
    23101 agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa atatgtcttc
    23161 tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga tctttatgcg
    23221 tatggatgaa tctattttac gtcatataga aggattagtg ggtaaatcaa tgacgaactc
    23281 agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt ttggctcaaa
    23341 agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg
    23401 aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt atgttaagcg tcgtgcaaag
    23461 cggcgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat tttgatcact
    23521 gtgttcgtga ccctatttct aatacttgcg gcgggctcga ttgataattc ggattcagat
    23581 tcaagcagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc gatttataaa
    23641 gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgccgt aggtgcagca
    23701 gtgaaatata aattattacc aagtgtgatc ctttcgcaat acggttacga atcagcgttt
    23761 ggtacatcgg cgtccgcaag aaatgatctc aactattttg ggattacttg gtttgatggt
    23821 tgtctgtttc ctaaaggaac tgcacgagga attggtggca ttgaaggtgg ctggtacatg
    23881 aagtttccca actcaaaagc tgcattttcc tattacggct ttatggtagc cacacaatca
    23941 aattttaatg cttgtgtagg aaataaaagc cctggcgcaa gcttattgat cttaggacga
    24001 ggcggttatg ccgctgcagg aattacggaa gatagtccgt attatacagg ttgtatgtca
    24061 attatcactt ctaataaatt aaccgaatat gatgaattcg ccatcaaaca ttggggtgaa
    24121 ggtggcaata acaacggcac gattactggt gaatggacga atccattccc tggcagcagt
    24181 ttagataaaa gttccttcag cggtggtcaa ttatttggaa cgaatccagg cggtgaattc
    24241 cgtccgaacg gcttccatga tggattagat tttggttcag ttgatcatcc aggaagtgag
    24301 attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat ttcaggtttg
    24361 ggcgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca agaatttgca
    24421 aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg tcaagtaatt
    24481 gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg gcgtcaagca
    24541 caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt tttgaatagc
    24601 agtaaaaaat agatcaggag aaaaaatatg gaaaataaaa cgttaaaaat cgtcttaaca
    24661 gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct gaaagaggaa
    24721 caagcagcaa atacctataa aattgaagaa gtggctaaaa acgcgtctga gaagctcaaa
    24781 aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat caatgagaaa
    24841 aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact
    24901 gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa atcattaagc
    24961 agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat cgacaaatta
    25021 agtcctacag acgatgatca ccaacatgat gattacggaa gttactcctt tacattaggt
    25081 gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt
    25141 gttgattata caatcggcaa tccgcaattc aaagatgtca aaccacaaaa aataaaaggt
    25201 ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcataga ctttagttat
    25261 ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gaaagacaac gtttcaaaaa
    25321 acgaaaaagt tatcttcaat ggctgggaat ctttatgatt ctcggctttt tgattcttgc
    25381 tagtccgatt tatacgaatc agaaggtaga tataccagat gaggtaagca cgaacgcctt
    25441 tttaaacacg aaagagctga caatggtttc aaaagaaaaa ggagcgaacc agcaattagt
    25501 ttttgaattt gcgatcgatg atgaagatca aagcttgtta aaagaactgg ctaatcttaa
    25561 ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa gcaatacgag ccaaagtgat
    25621 caaggtgtct gatgattatt ttgcagtgaa gctaacgcat gtaccagaag aatatatcgc
    25681 catgcgttta accattattc ctgaaaaaat tgatccaaaa gtggatatgc aagaaccaca
    25741 agatctgatt tattacgtgc acgaagataa ggttaaagat caagtgaaag atgaagatta
    25801 tgaacaacac gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa aagcagctca
    25861 aaaacaaatg gaatctttcc aagcaaccat tgatttgaat gaagaattag tgaagaagct
    25921 aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa acaagattaa
    25981 tagctaccag caagaaattg aaacatcgaa aacgcaactg aaagatcaag aagagatcat
    26041 tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aagatttaga aggacaggtg
    26101 gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat actttcgctg agcagaaagt
    26161 attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcgag aagtgaattt
    26221 atctaacaca acaatatctg agttgaagaa tggtcgaaag aatttagata ctttaaaatt
    26281 cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg ataacaaata
    26341 aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa taagctatat
    26401 caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct aacgcaaaca
    26461 tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa ccaaacagga
    26521 agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat atgacaaaat
    26581 atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca caaataacct
    26641 taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt gttaggtagg
    26701 ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat ttgtgtgtag
    26761 atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg tgtagcaaaa
    26821 tcaaaaaatg tgtagggata tgtgtagct
//
LOCUS       DACCWV010000024        26639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCWV010000024 DACCWV010000000
VERSION     DACCWV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.024"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(56..1087)
                     /gene="queA"
                     /locus_tag="IVW84_000906"
     CDS             complement(56..1087)
                     /gene="queA"
                     /locus_tag="IVW84_000906"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7917119.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1280..2455
                     /locus_tag="IVW84_000907"
     CDS             1280..2455
                     /locus_tag="IVW84_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7917120.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2459..3100
                     /locus_tag="IVW84_000908"
     CDS             2459..3100
                     /locus_tag="IVW84_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917121.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3097..4014
                     /locus_tag="IVW84_000909"
     CDS             3097..4014
                     /locus_tag="IVW84_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7917122.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4011..4682
                     /locus_tag="IVW84_000910"
     CDS             4011..4682
                     /locus_tag="IVW84_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917123.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5223..5711)
                     /locus_tag="IVW84_000911"
     CDS             complement(5223..5711)
                     /locus_tag="IVW84_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7917124.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5912..6178)
                     /locus_tag="IVW84_000912"
     CDS             complement(5912..6178)
                     /locus_tag="IVW84_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7917125.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6178..6441)
                     /locus_tag="IVW84_000913"
     CDS             complement(6178..6441)
                     /locus_tag="IVW84_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7917126.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6434..6706)
                     /locus_tag="IVW84_000914"
     CDS             complement(6434..6706)
                     /locus_tag="IVW84_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917127.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6917..7102
                     /locus_tag="IVW84_000915"
     CDS             6917..7102
                     /locus_tag="IVW84_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7917128.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7244..9067)
                     /locus_tag="IVW84_000916"
     CDS             complement(7244..9067)
                     /locus_tag="IVW84_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7917129.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9665..12370)
                     /locus_tag="IVW84_000917"
     CDS             complement(9665..12370)
                     /locus_tag="IVW84_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7917130.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12676..13623)
                     /locus_tag="IVW84_000918"
     CDS             complement(12676..13623)
                     /locus_tag="IVW84_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7917131.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13813..14082
                     /gene="rpsN"
                     /locus_tag="IVW84_000919"
     CDS             13813..14082
                     /gene="rpsN"
                     /locus_tag="IVW84_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7917132.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14258..14620)
                     /locus_tag="IVW84_000920"
     CDS             complement(14258..14620)
                     /locus_tag="IVW84_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7917133.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14631..15752)
                     /locus_tag="IVW84_000921"
     CDS             complement(14631..15752)
                     /locus_tag="IVW84_000921"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7917134.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15768..16118)
                     /locus_tag="IVW84_000922"
     CDS             complement(15768..16118)
                     /locus_tag="IVW84_000922"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7917135.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16248..17759)
                     /locus_tag="IVW84_000923"
     CDS             complement(16248..17759)
                     /locus_tag="IVW84_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7917136.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18081..19433)
                     /locus_tag="IVW84_000924"
     CDS             complement(18081..19433)
                     /locus_tag="IVW84_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7917137.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19586..20662)
                     /locus_tag="IVW84_000925"
     CDS             complement(19586..20662)
                     /locus_tag="IVW84_000925"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7917138.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20858..22603
                     /locus_tag="IVW84_000926"
     CDS             20858..22603
                     /locus_tag="IVW84_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7917139.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22883..23320)
                     /locus_tag="IVW84_000927"
     CDS             complement(22883..23320)
                     /locus_tag="IVW84_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7917140.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23304..24677)
                     /locus_tag="IVW84_000928"
     CDS             complement(23304..24677)
                     /locus_tag="IVW84_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917141.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(24999..25487)
                     /locus_tag="IVW84_000929"
     CDS             complement(24999..25487)
                     /locus_tag="IVW84_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7917142.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25509..26111)
                     /locus_tag="IVW84_000930"
     CDS             complement(25509..26111)
                     /locus_tag="IVW84_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7917143.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            26210..>26639
                     /locus_tag="IVW84_000931"
     CDS             26210..>26639
                     /locus_tag="IVW84_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7917144.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         7690 a         5624 c         4933 g         8392 t
ORIGIN      
        1 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
       61 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
      121 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
      181 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
      241 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
      301 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
      361 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
      421 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
      481 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
      541 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
      601 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
      661 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
      721 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
      781 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
      841 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
      901 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
      961 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
     1021 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
     1081 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
     1141 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
     1201 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
     1261 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
     1321 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
     1381 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
     1441 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
     1501 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
     1561 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
     1621 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
     1681 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
     1741 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
     1801 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
     1861 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
     1921 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
     1981 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
     2041 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
     2101 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
     2161 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
     2221 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
     2281 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
     2341 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
     2401 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
     2461 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
     2521 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
     2581 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
     2641 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
     2701 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
     2761 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
     2821 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
     2881 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
     2941 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
     3001 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
     3061 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
     3121 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
     3181 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
     3241 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
     3301 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
     3361 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
     3421 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
     3481 ccgtcctatg gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
     3541 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
     3601 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
     3661 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
     3721 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
     3781 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
     3841 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
     3901 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
     3961 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
     4021 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
     4081 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
     4141 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
     4201 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
     4261 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
     4321 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
     4381 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
     4441 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
     4501 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
     4561 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
     4621 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
     4681 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
     4741 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
     4801 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
     4861 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
     4921 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
     4981 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
     5041 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
     5101 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
     5161 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
     5221 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
     5281 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
     5341 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
     5401 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
     5461 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
     5521 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
     5581 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
     5641 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
     5701 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
     5761 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
     5821 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
     5881 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
     5941 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
     6001 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
     6061 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
     6121 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
     6181 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
     6241 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
     6301 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
     6361 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
     6421 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
     6481 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
     6541 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
     6601 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
     6661 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
     6721 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
     6781 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
     6841 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
     6901 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
     6961 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
     7021 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
     7081 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
     7141 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
     7201 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
     7261 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
     7321 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
     7381 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
     7441 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
     7501 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
     7561 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
     7621 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
     7681 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
     7741 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
     7801 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
     7861 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
     7921 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
     7981 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
     8041 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
     8101 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
     8161 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
     8221 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
     8281 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
     8341 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
     8401 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
     8461 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
     8521 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
     8581 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
     8641 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
     8701 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
     8761 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
     8821 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
     8881 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
     8941 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
     9001 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
     9061 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
     9121 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
     9181 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
     9241 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
     9301 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
     9361 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
     9421 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
     9481 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
     9541 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
     9601 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
     9661 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
     9721 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
     9781 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
     9841 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
     9901 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
     9961 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    10021 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    10081 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    10141 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    10201 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    10261 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    10321 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    10381 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    10441 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    10501 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    10561 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    10621 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    10681 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    10741 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    10801 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    10861 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    10921 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    10981 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    11041 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    11101 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    11161 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    11221 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    11281 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    11341 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    11401 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    11461 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    11521 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    11581 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    11641 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    11701 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    11761 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    11821 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    11881 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    11941 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    12001 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    12061 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    12121 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    12181 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    12241 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    12301 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    12361 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    12421 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    12481 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    12541 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    12601 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    12661 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    12721 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    12781 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    12841 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    12901 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    12961 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    13021 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    13081 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    13141 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    13201 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    13261 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    13321 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    13381 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    13441 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    13501 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    13561 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    13621 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    13681 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    13741 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    13801 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    13861 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    13921 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    13981 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    14041 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    14101 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    14161 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    14221 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    14281 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    14341 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    14401 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    14461 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    14521 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    14581 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    14641 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    14701 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    14761 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    14821 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    14881 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    14941 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    15001 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    15061 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    15121 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    15181 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    15241 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    15301 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    15361 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    15421 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    15481 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    15541 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    15601 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    15661 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    15721 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    15781 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    15841 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    15901 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    15961 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    16021 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    16081 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    16141 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    16201 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
    16261 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
    16321 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
    16381 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
    16441 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
    16501 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
    16561 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
    16621 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
    16681 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
    16741 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
    16801 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
    16861 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
    16921 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
    16981 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
    17041 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
    17101 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
    17161 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
    17221 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
    17281 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
    17341 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
    17401 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
    17461 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
    17521 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
    17581 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
    17641 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
    17701 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
    17761 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
    17821 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
    17881 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
    17941 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
    18001 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
    18061 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
    18121 cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata
    18181 gcatcaatca atttattctt ttctttttcc aaatagaaca caggtagttg ggggtatttt
    18241 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
    18301 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
    18361 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
    18421 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
    18481 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
    18541 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
    18601 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
    18661 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
    18721 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
    18781 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
    18841 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
    18901 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
    18961 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
    19021 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
    19081 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
    19141 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
    19201 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
    19261 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    19321 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    19381 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    19441 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    19501 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    19561 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    19621 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    19681 tattttccca taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt
    19741 cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc
    19801 cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg
    19861 gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct tcataatcat
    19921 agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta
    19981 atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat
    20041 actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc
    20101 cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac
    20161 attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact ggtacataag
    20221 gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac
    20281 atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt
    20341 ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt
    20401 ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg
    20461 ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt
    20521 tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa
    20581 tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc
    20641 catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt
    20701 actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc
    20761 cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt
    20821 ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag
    20881 ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca
    20941 gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa
    21001 gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt
    21061 caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga
    21121 aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc
    21181 ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt
    21241 taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg
    21301 agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact
    21361 ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg
    21421 cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga
    21481 cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca
    21541 tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct
    21601 gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg
    21661 ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga
    21721 acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc
    21781 ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga
    21841 ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt
    21901 agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca
    21961 agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta
    22021 ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat
    22081 ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg
    22141 gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc
    22201 gccttttgct caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag
    22261 cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca
    22321 tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga
    22381 caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc
    22441 tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt
    22501 cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc
    22561 aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga
    22621 aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg
    22681 aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa
    22741 aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc
    22801 ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac
    22861 tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca
    22921 tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact
    22981 ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc
    23041 cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt
    23101 gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag
    23161 ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt
    23221 ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt
    23281 cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat
    23341 aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag
    23401 ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga
    23461 tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg
    23521 tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga
    23581 atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag
    23641 aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa
    23701 gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat
    23761 ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa
    23821 agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc
    23881 cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc
    23941 ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca
    24001 agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca
    24061 aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg
    24121 aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt
    24181 gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac
    24241 aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct
    24301 tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc
    24361 cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc
    24421 aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca
    24481 aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat
    24541 ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata
    24601 aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt
    24661 ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg
    24721 attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat
    24781 acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca
    24841 agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga
    24901 aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg
    24961 acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt
    25021 ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca
    25081 aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag
    25141 atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc
    25201 aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt
    25261 atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac
    25321 acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa
    25381 tccacttgtt tttgaccaat gatcggcaca aaagcttccg gccatacttc ttgtatgact
    25441 gccatcagct catccagttc attttctgtt acttttattt tttccataca cccacgtcct
    25501 tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac
    25561 acacccaccg accatggttt caagaaaatc acaatctgca taaattccct aaaagataat
    25621 ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta
    25681 acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga
    25741 tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa
    25801 aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg
    25861 ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact
    25921 tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac
    25981 gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca
    26041 aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct
    26101 tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc tccttatgat
    26161 gtgcacccca aaagttagac tagaaatcta attaaaaggg gtgctttttt tgcggaaata
    26221 tacatttgtt tttaagaaaa aagtagtttc agactattta aataacgaag gcggctacaa
    26281 atatcttgca cataaatatc aaataaatcg tacactggtt agacattggg taaggattta
    26341 taactatcat ggttgggaag gcttggttgg aggtggcaaa agctacacta caaaatttaa
    26401 acttgatgtt atagaatata tggaaacaaa tggtctttct atccaagaaa ctgctaaaaa
    26461 atttaatatc ggttcaaata gaactctaag taaatggata gagcaatatg aagaaggcgg
    26521 tgcttcttca cttgagagcc aaaaaagggg cagaaaaatt agtatgaatt ccaagctaaa
    26581 cattcctaaa aaacttaaag atgagtctct tgaagaagaa gttattcgtt taagagcag
//
LOCUS       DACCWV010000025        25895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCWV010000025 DACCWV010000000
VERSION     DACCWV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.025"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(177..584)
                     /locus_tag="IVW84_000932"
     CDS             complement(177..584)
                     /locus_tag="IVW84_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7917145.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(773..1600)
                     /locus_tag="IVW84_000933"
     CDS             complement(773..1600)
                     /locus_tag="IVW84_000933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP7917146.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1909..2127
                     /locus_tag="IVW84_000934"
     CDS             1909..2127
                     /locus_tag="IVW84_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917147.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2148..2783
                     /locus_tag="IVW84_000935"
     CDS             2148..2783
                     /locus_tag="IVW84_000935"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7917148.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2976..3215)
                     /locus_tag="IVW84_000936"
     CDS             complement(2976..3215)
                     /locus_tag="IVW84_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917149.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3239..4762)
                     /locus_tag="IVW84_000937"
     CDS             complement(3239..4762)
                     /locus_tag="IVW84_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7917150.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4780..4902)
                     /locus_tag="IVW84_000938"
     CDS             complement(4780..4902)
                     /locus_tag="IVW84_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7917151.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4932..5915)
                     /locus_tag="IVW84_000939"
     CDS             complement(4932..5915)
                     /locus_tag="IVW84_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7917152.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5939..6088)
                     /gene="rpmG"
                     /locus_tag="IVW84_000940"
     CDS             complement(5939..6088)
                     /gene="rpmG"
                     /locus_tag="IVW84_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7917153.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6109..6486)
                     /locus_tag="IVW84_000941"
     CDS             complement(6109..6486)
                     /locus_tag="IVW84_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917154.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6518..6697)
                     /gene="rpmF"
                     /locus_tag="IVW84_000942"
     CDS             complement(6518..6697)
                     /gene="rpmF"
                     /locus_tag="IVW84_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7917155.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6712..6981)
                     /gene="rpsN"
                     /locus_tag="IVW84_000943"
     CDS             complement(6712..6981)
                     /gene="rpsN"
                     /locus_tag="IVW84_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7917156.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7504..9276)
                     /locus_tag="IVW84_000944"
     CDS             complement(7504..9276)
                     /locus_tag="IVW84_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917157.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9230..10984)
                     /locus_tag="IVW84_000945"
     CDS             complement(9230..10984)
                     /locus_tag="IVW84_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917158.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11094..11663)
                     /locus_tag="IVW84_000946"
     CDS             complement(11094..11663)
                     /locus_tag="IVW84_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7917159.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11681..13105)
                     /locus_tag="IVW84_000947"
     CDS             complement(11681..13105)
                     /locus_tag="IVW84_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7917160.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13107..13775)
                     /locus_tag="IVW84_000948"
     CDS             complement(13107..13775)
                     /locus_tag="IVW84_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7917161.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13906..14490)
                     /locus_tag="IVW84_000949"
     CDS             complement(13906..14490)
                     /locus_tag="IVW84_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7917162.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14825..15055)
                     /locus_tag="IVW84_000950"
     CDS             complement(14825..15055)
                     /locus_tag="IVW84_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7917163.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(15070..16020)
                     /locus_tag="IVW84_000951"
     CDS             complement(15070..16020)
                     /locus_tag="IVW84_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7917164.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16017..16859)
                     /locus_tag="IVW84_000952"
     CDS             complement(16017..16859)
                     /locus_tag="IVW84_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7917165.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16992..17717)
                     /locus_tag="IVW84_000953"
     CDS             complement(16992..17717)
                     /locus_tag="IVW84_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7917166.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18178..18492)
                     /locus_tag="IVW84_000954"
     CDS             complement(18178..18492)
                     /locus_tag="IVW84_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7917167.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18508..20103)
                     /locus_tag="IVW84_000955"
     CDS             complement(18508..20103)
                     /locus_tag="IVW84_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP7917168.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20126..21016)
                     /gene="iolE"
                     /locus_tag="IVW84_000956"
     CDS             complement(20126..21016)
                     /gene="iolE"
                     /locus_tag="IVW84_000956"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP7917169.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21033..22058)
                     /gene="iolG"
                     /locus_tag="IVW84_000957"
     CDS             complement(21033..22058)
                     /gene="iolG"
                     /locus_tag="IVW84_000957"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP7917170.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22082..23089)
                     /locus_tag="IVW84_000958"
     CDS             complement(22082..23089)
                     /locus_tag="IVW84_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7917171.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23202..24362)
                     /locus_tag="IVW84_000959"
     CDS             complement(23202..24362)
                     /locus_tag="IVW84_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP7917172.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24438..25892)
                     /locus_tag="IVW84_000960"
     CDS             complement(24438..25892)
                     /locus_tag="IVW84_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP7917173.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7944 a         5295 c         3975 g         8681 t
ORIGIN      
        1 ataagtaatc taaccgactg ttaactaaac cattgcaggt cccgttaaag atacttgtgc
       61 ctgttgtcac cgcttctttt tcttgtccac aagcgcttaa cacacttaat aaagcccctg
      121 ctattattaa taataatatc tttttcttat taaaaacctc ctcatctatt tccagattag
      181 taaatgtagt aatctttgct ttcttttttc tcaatactaa taactaactt atggggtaaa
      241 caaatactcg tttgaccggg ttttgagatc catccagttc taaccgcgat ttgatcagga
      301 ctattatctt ctttaactcg aattcttcca tctttaatct caacaatatt atattgtccc
      361 ttcgcaggat gataattcac gagtttatga ttgatcttat ccaaatcgaa gcgatccatt
      421 tccttgccat caatttgaac gatggcatat ttgatagcat cttcagttgt ttcattctcc
      481 ttttctatag agaatacaaa taagggaata aaagagggaa taatcaaaat gccaataatt
      541 atgacatcgc ctatcttgac accagctttt cttaaaaatt tcattaacga tatctggcta
      601 aacatattag aatttttcca tggaaatggt gaaatttttc ggaaattatc tgcattaaga
      661 gaatcagtta aacaaccctc aagttgttcc ttaatacatt gttgatccat cttcatacca
      721 ataaaaacta gtttattaat gcgttcacca aacaacggat gccaggcttc cttcaattcg
      781 ggtttttttg tataatcatt tgacactcaa ttttaggtaa actagctacc caataattta
      841 atgattcaat acttttatgg ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt
      901 gggcaaacca aagaaaccct tttgctcttg taatattgcc aggaaaattt ttatccgtaa
      961 gatgggcaaa tttttgtgcg tcaaggagta accgatttcg atagataaaa cttgagatac
     1021 catattcttc tgtttcagga atatgatttt gatagccaag ctctaactct ttgaaccagc
     1081 ccattgaata ggaagtctca tctatattaa aaagatttgt atttagaatt ttcgataagt
     1141 ctacttcacc tttaattgtc ctaattattt tggcattgga ctgtaactta gcaattagac
     1201 cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact attatttaaa agtaatatgt
     1261 tacaacactc gaattgatca attaacaagc cacgaatatc ctgttcttct tcaactacat
     1321 taccttcagc ttttctttcc tgaatctttt cacccgtttc gaaattatcc caaaaacgtt
     1381 ttacatccac tactataata atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta
     1441 aagggttttt agctaatata attgcttgaa taattggaat tggtttgctg attcctgaag
     1501 cttcaataac aatttgattc aagtcagaaa attgcactaa ttgatctagt tcaacaatca
     1561 aatcttcgcg taatgtacaa caaatgcatc cattcggcat cgaaacaagt ttttctccag
     1621 ttcttttaaa acctttcttg tcgactaata ccccatcaac atttacttcc cctatgtcat
     1681 ttacaatgac agcaatttcc tttccgtgat caccgtacag aatatgattt agaagtatcg
     1741 tttttcctga accaagatag ccagttaaga ttgtaatagg tattttttca tagggaacct
     1801 ctttctatac cttgaattta cactttaagt gcaacacata aaaatgagtc ataatcgata
     1861 tccaactagt ataagtttgt cgagaacact cctagaagga aaataattat gagccagtat
     1921 tgtcatctta ctatactcaa acgagaaaga atctttctgc ttcacgaaga aggattttct
     1981 attagtataa ttggcaacga cattcagcat agcccgttta ctattttaca tgaactgaga
     2041 agaaacaaga aaaaaactaa tcaccttcca aagcggaaaa aaattatgct tatcgaagaa
     2101 aaaaatgcgg acgttatcac attctaaatg atccgtgagt ttggtcaatt gttcgtcatt
     2161 tatttgtcga ttttcagtgg tcgccagaga aaatctcgaa tcgtttgaag catgaacata
     2221 tggacttttc aattagtttt atcacgatct accgtgcaat ttgcaatgat cttttcgata
     2281 cagaacccct ctcacacggc aataaaggcc tgatccgttc attgcaccat cgaggaaaaa
     2341 caagacatac aaaaaatcat gtcgagtctc gtgggaagat ttgccttact cattcgatcc
     2401 atggccatcc aaaatccgca gatgatagaa gtgattttgg tcattggaaa gccgatattg
     2461 tagctggcaa aactagtggt acttgtttag tgactttgac agacagaaaa tcatgcttcc
     2521 ttttagcagg aaagaaggtc cccagaaaga aagccgctta tgtgagcgag aaaatgattg
     2581 aacttttaag cagtattcca aatgaggcgg tcaaaacgat cactccagat agaggaaaag
     2641 aattctccaa tcatttcgca gttacagaag tattgaataa tatgcccttt tattttccag
     2701 atcctcatgc ccttggcaac aatgaacaaa ttaaaatacg aatagactca ttcgagaata
     2761 cttgccaaaa tcgaaagaga tagctacggt agataactca acgataaatc agatgattga
     2821 aaaactgaat aacagaccta gaaagtgttt agattggaga acaccttatg aagtcttctt
     2881 tgataaagtg ttgcgcttga tttgacaatt caagaaataa taaaaagcag atcaaatcca
     2941 aataagggaa aaatgatcta ctctggtatt atcaattaat tggccaagct attatgccgt
     3001 tttgctaaac gtctacttct tccagtattg accttgataa aacaacaatc aggatcccgt
     3061 tttaatactc tatgaactgt gtcaatacta acatgtaaag ttgcagcaat atcagctaat
     3121 gtactcccat ttaatctcat ttgctttgca aattgataac gccgataagc atcaatattc
     3181 ttggaatcca tagacaataa tacttttctt accatgtatt tcctccaaag attggatttt
     3241 aatgggccat catttcttga gcaatctcat gctcagataa tgaagctgga tagaaagtag
     3301 gccaattatg catttcatta taaagttttt cttgactttc tccaccaaag aaaatatgga
     3361 aatgctctgc cttacttggt gcaattccat gatcgctaaa ttgaatatat ttaaaacgac
     3421 cagcacctgc atcatcagtt tcaaaattaa aacgaacacc acggtttcct ttttcataat
     3481 tcaaaatttt gtatccaaca tatttgtatg tgtattgatg atgttcaccg ttgactacaa
     3541 aatcaatggt gttgtcttta atgttgatag tatcaatatc tgtcttatag ccagtagtat
     3601 aataatcttt gtactcggca gctgtcatgt ctttattgag ttttgctttg taatcgaaaa
     3661 cttcatccaa aattccatct tttaacaacg gataaacaga ttgccattcg cctgtataat
     3721 cactcaattc gcgatccttc acatcggcat ctttaaagta cccattagca actgtctttt
     3781 catccttcgc ttctttttct ggaactacct cttcaccagc aaccatagtt gttttctcta
     3841 acgcttttag attgtcttcc attactgaaa cataatcttc accattatcc atttgttctt
     3901 ttgtaagact ttcaagagga ttcaatactt ccaattttat cccagcttca ttggccaaag
     3961 tttttgcgat tttatcatta gcattctttt caaagtaaat ataattgatc ttattttttt
     4021 taacatactc ttttaattca gctaatcgac cagaagatgg ctcttcttca ggacttagtc
     4081 cagcaatagg aacttgtatc aatccataat ccaaagctaa atatccaaag gctgcatgtt
     4141 gtgtcacaaa acttttttgt ttcgcttctt taagactagt ggtatagtca gcatccaacc
     4201 gtttcagttt agtcaaatac ttttcagcat ttgtctcaaa tacttttgct tttttcggat
     4261 acaatttaac taactgatcc ttgatatttg aaacttcctt aattgccatt ttgggtgaaa
     4321 cccacgtatg aggatctagt tcatgatggt gatcttcttc tccgtgatca tgatcttctt
     4381 cttcactacc tggcaataaa atcatgtctt ttgttccttc tactacatta ggcccagctt
     4441 tcttccaact ttctttagct tttggtaccc atgattccat gttttcgtta tgatatacaa
     4501 aaacatcagc atcatgagct tttgccatat ccttagctga gggctcatag tcatgaggtt
     4561 ctgatcccgc tggaaccatt aaatccacgt tcgcttcatc gccgacgatg tttttggtga
     4621 aatcatacat tggatagaat gttgttacaa tttctaattt tccatcttta tcattcgata
     4681 cttttgcatc attagtgctg gagcaagctc ccaataatcc tagagctgct atcccaataa
     4741 gtcctacact gattttcttc attctttttc cttccttaat tatgctaatt tcttcatttt
     4801 attttgttta tgttctttga taatttttaa tgcccttttt ctagttttga tattcgggct
     4861 tttaagtcgt cgatatgcac tagatatatc cgttttctcc attagaaatt acccctaatc
     4921 aatacttatc attatttgct ttccgccagt aatttatcaa aactctgttt gatcctttct
     4981 tcatccaaca tttttccaat tagaataata gtggtttggt ctttttgtcg agtggtgtat
     5041 tgaaaattaa cctgttcgtt taccccttgt atggcaacta taaaatcttg atcttgcaaa
     5101 gcaatcaatc ctttaaaacg atacaggctc ccttggttgg ttaaaatcag ccggtcaatt
     5161 aattggaata aaaaatcttt ttttatgtct tgatctgaag ttaagagaat tgatttaaac
     5221 tcatgttctt gatgatgttg atgatagtgt ttgccattat agtgattttc tgatgaatca
     5281 ctttcaaaaa ctaagggatg attgaatttt tccaaattat aaaaatcctt tttttgaatc
     5341 gtttcactat gtgtgaacat tcgaatatcg gataaagggt taatactttt gatttctctc
     5401 tgtaattttt ttagtgcaga taaattcaag ttttgttttc tagacacaaa aatgcgatct
     5461 gctatgacaa gttgcttaag ggcttgtggt tctctttcaa taagttcaaa actgtcacta
     5521 tcaataacag tcaataaact atcgatatat actcttcctc ttagttgagg cgctgttaaa
     5581 agtgtttgaa tgattggttg gggatctgct atacctgtag tttcaatgat gatttgtgaa
     5641 attggttgtt gatgttcttc tattgtttcc agtaaagaaa tcaatattgc taataaatca
     5701 ctgcgaagac tgcaacacat acatccatta tttagttgta acacccgttc ctcgctatga
     5761 actaataatt tgtgatcaat cccaacttca ccaaactcat tttcaattat aataatttct
     5821 tccctggggt agggtgagac ttgcaaggct tgattgatta gcgttgtttt tccagcccct
     5881 aaaaacccag aaacgattgt aactggtatg cccattttgt caccttttta ccacttagct
     5941 atttcacttc tttgaaaatt gcccgtctgc gcaattttgg cgaatacttt tttaattcta
     6001 accgttcagg gttattccgt ttatttttgc tggtgagata cagacgttct ccagtttcga
     6061 cacattccaa aataatattt tgacgcataa atcttcctcc ttcaatactt atcgatcttc
     6121 aagatatgct tgaatttgtt gtgcatcttc aaaagttaac tcgccagatt ccaattgtga
     6181 aactaaattt tctttcatat caacaggtat ctcccaataa ccatcaatac attgaaataa
     6241 ctcctcgtca tactcttcaa aaattggata gattcttaaa aaatcatcaa atgataagcc
     6301 ttggtcctca tagtaatctt tgactaatcc attttcaatt gcctgttggg tcggtaaacc
     6361 cgtttcagta ataattcctc tctctaccat cgaacgataa aaagtgcatt tatctagcga
     6421 ttgctgggtc gtttcaagat aatactttaa tccttcttca actaaaggtg ggtaaataat
     6481 ttccattttg attccctcct aaaagttata tattaaacta ttttgaagct cctttaatta
     6541 ctttctttcc attatagtac ccttttaaag atacatgatg gctacgtcta taatcaccat
     6601 tcgcttcgtc aaatgaaatt tctggtttta ctacagtttg atgagttctt cttaaccgtt
     6661 tcttcgcctt agatgttttt cttgctggta ctgccatgat atttcctcct tttaccaact
     6721 tgcttttttt actccaggaa tcaacccttg atgggcaagt tctctaaact taatacggga
     6781 cataccgaac tttcgcatat accctcttgg acgaccatcc gtctgatcac gaagcctcag
     6841 acgattcgga tttgaatctc ttggcaattt agcgagagcg gtgcgatctc ctgcggcttt
     6901 taattcctga cgtttatctg cgtacttttc aatcatcgcc atttgtttct ttgctttagc
     6961 aattttcgac ttttttgcca tataccttta tactcctttt attttgattt agacatctca
     7021 aatcgtaatc gttacgtttt gacactgata ctttaccaac aagagttgct cttgtcaaca
     7081 ataaataaca tgaaataaaa ctcatatgaa tagataacta tttgaaaacg aatttatcgg
     7141 tacatagaga taatcaaatc atttaaaaga atatgcagtt taagctctga aaaataagaa
     7201 ttcactttta ttagtccaat caaacacaaa taccgtttta agtagctttt cctctactta
     7261 aaacggcaat atgaaagaaa ctataacttt ttattcaatc ttttatgttt aactggttta
     7321 tcgccaattt tttaaaactt tgattattcc ttatattata agaatagaca ttaagtgaac
     7381 taattcaatc atactgattc tttcgtgtat tcaaaattag atgctgttta ataaaattat
     7441 tctttttgaa aaagaattat aactatatcc caaaaattgt aggttaattt aaagcctatt
     7501 tcatcatcgt gattgagctt ctttttcata gacgaatttc tcagatagtt tagttttctt
     7561 tatgagattt ttgtatattt ctgaccttcg atacaactca ctatgagtac cttgatcaac
     7621 tatttttcct tgatccatca ctacaatttt atttaccttt tcaatagact tcaagcgatg
     7681 cgatataatt agtactgtct tatcttgtat taaacgattt aaactttcct gaatacgttt
     7741 ttcattttcc gcatctaaac tggcagtaat ttcatctaaa attaaaattg gcgcattttt
     7801 caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg aacttccgtt
     7861 ttctccgacc aatgtatgat aaccgtcagg tagtttttca ataaagtctt cgcagttagc
     7921 taaacgagct gcttttttca cttcctcatc tgacgcttga ctatttccaa tacgtatatt
     7981 ttccaaaata cttgtattaa acaaagttac ctcttgaaaa acaatagcga ttttgctaaa
     8041 caaagaagct ggagatacac gtttaatatc atacccatca attaaaatac agccttcatc
     8101 ataatcatac agtctagaca ctaatttcaa gatactagtt ttcccggaac cactagcacc
     8161 tactaacgca gtcacttctc cttgtttagc cgtaaaagag atatgatcta aaattggggt
     8221 attgttatca tatgagaaag agacatctcg caactcaatg tcaaaagatt tcagaggact
     8281 atctgatccc tcttggatac tggtttcctt catagctcta attcgctgta ttttaggagc
     8341 taaataaaat atttcaagca ctgcttcttt catagaatcg aaggaatcct ttatttttat
     8401 tgcggctagt aagtaaccaa cgacgtagag tatagtcact tcacccgtta acagtaagtg
     8461 aacacctact agaatgacta ctgctaaact aacatatgag aaaatagagg ataaggccat
     8521 gactgaaaaa gtacttaatt ctacctttaa atgaatcctt tcactctctt ccattttttt
     8581 gaaaagtctg tcttgaactt ttttagatag attaaagcta ttaatctctt gctgcaattc
     8641 tatagtttct tgaaattctt ccgagttttc tctcaaagta tcgtaatatt tagtattggc
     8701 tttcgtttgg gatttctttg ataacaagat taagacaaaa ctaaataacg ttggcaaaat
     8761 aacagctaat cccattttga cattaccaac caaaagcatc actgaaataa aagggaaaaa
     8821 cagagccata ccgccggctt taggtattgc atgactcatc gcatgctcaa taccttcaac
     8881 gtcagacatg atagtttgag ataaatctga taaattatgt tttgaaaaat aggataatgg
     8941 taaatttgac aaatcgtctg caatttgcat tcttaaatgc gcactttctt tataagtact
     9001 attatataag ttttcatact ccctatctaa caaccaatac atgaaaagca aagtaacgaa
     9061 cgatactgta aaataaagcc aaggcggctt tacattttct aaaactaatt cgtctatcag
     9121 catcattata atgaatgctg gtaccatatt aatgcagtat acaaaaaagc tagaaataga
     9181 tgctttaacc aaatcttctg cgccctgatt agttaaagcg aatttgtttt tataaaaact
     9241 tttcattgtc aagcctccaa tcatttgcat tctcataatt agtgaccaat tgtttataca
     9301 cttgatgttt agccattaat tcattatgat ttccacgttc tactattttc ccttctgaca
     9361 agacaagaat ttcatctaca gatttaatgc tggataatcg atgagctatc ataataactg
     9421 ttttttcttt catcagatgt tgaaaagcct tttgtagttc atactcatta tctggatcaa
     9481 tagatgcact ggcttcatcc ataataacaa tctttgcatc ttttaaaatt gctctggcaa
     9541 tagctatacg ttgtttttct cctcctgaca gataaactcc tttactacca attaaggtat
     9601 gctctctttc gggaaatttt tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa
     9661 atactttact tgctggcgct tgaggatttg ctatagaaac gttatcgtaa atactctgat
     9721 tgaatagttt cgtatcttga aagacaaaag caattgtgtt aataattgtg tcttttgaat
     9781 agtgatttaa aggcttacca ccaattttaa tctgtccctt atccacattg tagaaacctg
     9841 aaattagttt agctatcgta ctctttccgc ttcctgattt tcctactaat gcataaattc
     9901 ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc
     9961 tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt
    10021 ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa
    10081 acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa
    10141 aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa
    10201 aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa
    10261 aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt
    10321 tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact tgaattcctc
    10381 ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa
    10441 aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta
    10501 gaaccaatcc aattttaaaa ttaatgaaaa agcctaaggc aactgataaa acaggtgtta
    10561 ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt
    10621 tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg
    10681 cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga
    10741 tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag
    10801 tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga gacttaagaa
    10861 atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag
    10921 tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt
    10981 tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt
    11041 tccgtaatag cgtaaattag agatgtacaa acagatagaa ggtaaactac gttctatttt
    11101 ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca
    11161 actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt
    11221 gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata
    11281 ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga
    11341 attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc
    11401 gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca
    11461 attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg
    11521 attccagata ataatccaaa aatccctggg gatatgctgt gaccaaacgc cccaccaata
    11581 ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc taaaccaata
    11641 aaaatataat cttttgtttt catatatatt ctcctcaatt ctatttcgtt atgtcattaa
    11701 aaatctgttc tatcaaagtt gaatcttcta ctctaagttt tatatctttt tttattccac
    11761 cattttttaa ataaataatt ctattgcatg ttttataaag aaattccgga tcatgagtgg
    11821 ctataactac tgcacccttt tcttttaagt ctttaataag gtcagcaatc ctttccatat
    11881 taatataatc caacccactt gtaggttcat caaatagaaa taaattagtt tttgacaatg
    11941 aagctataga aaccaataat ctttgttttt ctccaccgga taaatcaaac ggtaattgat
    12001 ctttcatttt ataaagttcc atttttttta aaattttttc ttgctgatct ttactaacta
    12061 tttcattccc cagggctaac tcgctcctca ctgattctgt gaaaaattgg taatccattt
    12121 cttgcattac taaaaacggc gatgacaaat tttttttatt ttcgattatc tttcctttgg
    12181 tagggcgaat aatcttcatg agtattctta gtaaggttgt tttccccaca ccgtttttcc
    12241 ctaccaaacc aataacatct ccaactttga catcaaaatt tatgtctttt agaatatttt
    12301 cataagtaat ttcttcaact tttaatacat ctgcacttac cttttcgata tagggaactt
    12361 caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca gtttttaaca
    12421 actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga tcaattaatt
    12481 ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta ttttttaaat
    12541 cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa gacggttcat
    12601 ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt
    12661 caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag
    12721 ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa
    12781 atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt
    12841 agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata
    12901 aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta
    12961 atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagacta ccttttttta
    13021 ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat
    13081 tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg
    13141 gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc
    13201 acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt
    13261 gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac
    13321 aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc
    13381 ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt
    13441 tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt
    13501 acgaccggca acattcgaaa tacaatatat aaggatgtcc caaaaaatat agtaaaaaac
    13561 tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg
    13621 aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa
    13681 caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt
    13741 aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc
    13801 gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc
    13861 aaaaagcata actgagaaca attctcaatg caagcttttt tttaattaac tcagtaattc
    13921 aatccaccca ggaacagaaa attgatacaa tcttctggtt aatagcatag tcttttcttt
    13981 tgaatagcta tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa tgtagaagtg
    14041 gatttcttct tcattaaaga ctaaattttg attgatcatt ccgatagtct ttaacttatc
    14101 tagacaacag actgtctttt ttgtcacaaa tgatgtaatt ttttcaacaa tatcttgata
    14161 tttggttcca gctgattgga ataaaaccaa atcgaatata cgtttattct caaaaatata
    14221 attaataaat ggatctaatt cattgaagtt ctctctccaa cttttctcta tatctatatt
    14281 ttctaagttt aagaggtatt tggtaaactc ttcattatac atattaataa gcgcattaat
    14341 atgcggctga ataatttctt caaataacgc ttccttagaa gtaaaatgtt tataaaaagc
    14401 acccgtagtt acgcctgcgg tcaagcacag ttgccttatc ttagtttgct gaaatcctac
    14461 tttttcaaaa tggcttagtc cactttctat taatttttgg tgagttgtaa tataatctct
    14521 ggtttctatg ggtacttcct cctaataaga tgaattaact atattatctt acacaggact
    14581 tgaagtttca atcgttaacg tctattactg aactcattcc ttagagctaa actaatgaga
    14641 aagagcaaaa taactgatac aaaaagcaat cctacaagaa ttaatagact catctaaatc
    14701 attcaccttt tctaaaataa tccgagaaag atttaaaaaa attatatcca gctattagga
    14761 taagtcctat cataatcact ccaatagcca ttttcttatc ctccctaaaa ataaagcctt
    14821 ttacttaaaa cctttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
    14881 atcctatatc taaatagtcc tcatatctaa gtttagtcag catctcactt acagatgctg
    14941 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
    15001 attcctgaat tgctagtaaa taaattagta tagtagttgt tttaatttgt accataccta
    15061 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
    15121 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
    15181 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcgtcca cactgctttc
    15241 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
    15301 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
    15361 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
    15421 tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc
    15481 tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat
    15541 atttttagcg taaataatac cgttttctaa atttaaccaa gcatggggat cttccttccc
    15601 tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc
    15661 tttgttttcc tctttgttcg cattttttac taattttgta aaccaagcat ttcctccagt
    15721 ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc
    15781 aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat
    15841 tttatctttt gctatatttt cagtaatatc tgctaaaatc gaattagtaa ctactacctt
    15901 taatttttcg ttcgaagcca ctgtatcttt gtctttacta tttgttgaac atgccccgaa
    15961 tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat
    16021 aactttctct ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca
    16081 aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca
    16141 atgaacaaac ctagcaatga tgagcacgct ccgactgtcg ccgaaataaa tatcatagtt
    16201 tttaaactgt ttgcatataa atatgctgta gcagctggtg taattaacat tgcaacaatt
    16261 aatatagttc ccacactttg catagctgtt actgctacta aagttaatag aatcattaat
    16321 agataatgat aaaaactgac attcatcccc attgcttttg ccattaatgg atcaaaagaa
    16381 gtgattaata attctttaaa aaatagaaca actgtaacca acactaaaac agaaacgcca
    16441 attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa gatatgaaat
    16501 aaatccgttg agctatttgc aactcctata aggataactc ctaatgctaa aaaagaactg
    16561 aaagtaatac caattgctgt atctcctttt attacactat tgctttttat aaaagtaata
    16621 atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata
    16681 taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt
    16741 cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta
    16801 attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata
    16861 ttattcccct tgtatattag agttagggtt ttaaccttcc ggttttgatt ggataaccct
    16921 atgaaccatc gttattttaa tttggtatta actcatttct tttgagataa tatatttatt
    16981 ggattgagag gctaagccgt tcccccctct ccaataaata tagtatctcc ataggctttt
    17041 tttaaattct cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc
    17101 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
    17161 actgtttttc cctgatgttt caattctttt aacgtatcca taattattcg ttcactaact
    17221 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
    17281 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
    17341 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
    17401 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
    17461 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
    17521 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
    17581 gctttaagca atgtagattt tccagcacca tttggaccaa taatacctgt taaagcacct
    17641 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
    17701 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
    17761 tatacaaaaa ttatatttta gtcaacccta aattttataa tttagatttt tttctgttgt
    17821 tgaagattag gatgtcaaaa ttatttgaaa ctactcgtat agtaaatagt aagtaatttt
    17881 caaaacacga agaaaaacaa aaagaaaaaa ggaaacgccg aagcatttcc tttcagtgat
    17941 ttaatgagaa caacacgatt tgacttagag tctcaaaaag tcgaataggt acactcattt
    18001 aagtgtctcc attcgacttt ttggggatga gctggctatt gaggtccgct taccctattg
    18061 aataaataca agattgggta tttctaataa gtccgccctt catattgggg tgatcggggc
    18121 gaacatgcca tcttttcata taccacctcc tcgacactca ataatcaaag attctcgtta
    18181 atcttcttcg atgcttgcaa tgatgttttc aatattcata taacgaaaat cttcttgttt
    18241 gatataaacg atctgagcgg tacatttttc ctttttgatg atttctttta ctttttcctc
    18301 agaacgtgcg acctgcggcg ttaaaagtaa aacgtcatag tctttataat tcagtttttc
    18361 ttcaacgttt cggatcgttg cataatctat ctcatacgat agatttttgg ctctgatcgt
    18421 ttcaagcatt tttgatacga gcagcgacgc actgattccg gtctcgcaaa taatcaatag
    18481 atccttatgc ataataccct cctgttttta gcctgcaaag ccaattggtg aaaatgccac
    18541 ataagttaaa ataacaatga ctaccaatgc tatagcagtc accttggtgt atttccaagg
    18601 ctcgatatcg acctttgtat cgaatgagtt caacacaaat tctccatcag gtttcaattt
    18661 ggaaaccacc atcaaaatga taatgtccag aacgaataat gccgtcaaaa cataaaggaa
    18721 atggatattt tggaaaagca tttttgatag tgcatacaag acgatatgca cggcaatcgt
    18781 cgtttttgcc ccaaaagcag ttgctttttt tgaataaaag gcaaagagaa tgatcacgag
    18841 cagcggcatg ttgtatagtc cattgaattc ttgaacgaac tgatacaatc cagccggtgc
    18901 aaaagaaatg aatggcgcta cgataatgga gataatcccc acaacgaccg ttactgtttt
    18961 accagcacga gcgatttgct tatcatttgc tttcttattg ataagcggtt tataaaaatc
    19021 cagtgaaaac aaggtcgatg tcgcgttcaa agctcccgca aatgatgata agatcgcgcc
    19081 aaagataaca gcggcgaaaa taccaaagat cattttaggc aatacttcac tgactaaaaa
    19141 aggatacgcg ttatctggat ttgacattaa actatcgcca aaaatattgc gagcgacgat
    19201 ccctggaaag acaaggaata acgcacctaa gattttgaag acgccgacta ggaaagcgcc
    19261 cttttgtgcc tcagctaaat tcttgccgga caatgctttt tggacgatca tttgattggt
    19321 acaccagaaa tacagattgt taaacagcat ccctgtaaat agtgtcggcc atggaacaac
    19381 agccgagtcg atcgatccca cagagttaag cagccatggc gtgtttacca caacgtgttc
    19441 gatcccgcca acaaagctgc cgtcaccaag cttcattaag cctaaaattg ggaccagcag
    19501 accacaagcc agcagaccga ctccataaat ggagtcacta ttggcactta acgacaagcc
    19561 gccgatcagc agatagagga tgccgacaat accaattgcc atcgctacaa ttatgatcgc
    19621 gacgatcggc tggacgccca gcagctcatc aatatggaag atcttattga acaccagcga
    19681 tccagaataa agaaccacgg gtaagaaaga agtcatatag gtcacaataa ataagatcga
    19741 aataattcgt ttggtaaccg tatcataacg aatctcgatg aaatctgaga ttgtattgat
    19801 gccgtattta aaatattttg gcaagaatat cacagccaaa gctacgatcg caatcgtagc
    19861 agttacttcc caggccatta cttccatccc tgcatgataa ctttgaccat tttgtccgac
    19921 tatttgttcg gtggacaggt tcgtcatgat gatcgtgccg gcgattggca aagcagttaa
    19981 gctccgccct cccatgaaga acccttctga tccagaagta tcgacacttc tggatctaga
    20041 ataggcaaat aaccatacac agatgacaat cactaggaaa ctgattatag aaaaaagatt
    20101 catcgtctag ctccttttca atagattaaa gaccggtgac ctctcgaatg tagcttctgg
    20161 ctttgactgc atattcaaat ggattggctt tagccgggtc ctgttctgct tcaactacga
    20221 tccagccttc atagtcgctg gcatggattg ctgtccagat tggagtgaaa tcaatcatcc
    20281 catcaccagg aaccgtgaag acgcctttct tcacgccgtt taagaagctg tctttttcct
    20341 tttctacttc ttcagccgtt tcttgacgaa tatctttgaa atggatgtgt ttgatacgat
    20401 cttgatgacg gttgtaaacc gcgattggat tttcaccaga aaagaccaaa tgtcccgtat
    20461 cgaacaataa tttaactttg tctggatcag tcatttccat taaacgatcg atttcttccg
    20521 tcgtttgaac gccggtaccc atgtgatgat ggtaaacgat ctccatatcc ttttcgtggg
    20581 ccaaatcccc taatttttct aatccttccg ccagtttttt ccactcttca tcagtgaaaa
    20641 ctggtttatc atcaaagact ggcgctgtca taatcccttg gatactatga ccctgctcag
    20701 ctactacgat gactttagcg cccatcgcat gtaagaaatc ccgatgtgct ttgaaggctt
    20761 cggcagtttc ttcaaaaggt ttagttgtca gaaacgcact gaaccatgca ctggcaacct
    20821 ttaagccgcg aatattcaaa tattgttcca acacctctgg atcttttgga tatttgttgc
    20881 caatctctgt tccggtgaac ccagcaaggg ccatttcact gatacattgt tcaaaggtat
    20941 tttcactacc aagctcgggc atatcatcat tggtccacgc gattggtgcg atggctaatt
    21001 gaatttgttt ggtcatattc tcacactcct gcttattttt ttagttgtac taaactcttc
    21061 gtatcgaatg attcttgaca agccaacgcg atcttagttg attgcaatcc atcttctgca
    21121 ttaaccgctg gttgacgttt ttcttgaata catgagacaa attcttgggc ttcacgaatg
    21181 aaggcttctc tgaatcgttc tgggaagttt tgtgaacatg gacggatgat cccttgatcg
    21241 ttcatgatcg tcacaagatt tttctcaggc gcttgcgcta cccgcagcat tcctttggtt
    21301 cccatgatct ctgtttcgac atgatagcca tgggctgcgt ttctgccggc aactagtaaa
    21361 gctattgttt tatcgctcag gctcatcatt gcggctccag tttcaagctc accagcctga
    21421 tccaattctg gatacgccgc atttttccca atcgcccaca cttgatcgac ttcttggccg
    21481 gtaaaccagc gaaccaaatc aatatcatgg atcgccatat ctaagaacaa gccgccgctg
    21541 tcgttattca tggcaaattt gacaaagctt tccaagcctt cgctcggatc gatcccataa
    21601 caacgaatca gtgtgatttc gccgatttcg ccatcttcta ccattttctt cgcatactga
    21661 tacgactcat catagcggcg catgaagcct aacatgaaga cctgttcagg atgctcttcg
    21721 atgacttttt gggtcttttt aatatcttca acttcaaggc ctaatggttt ttcagtaaag
    21781 acatgcttgc cattttccat cgccaagcgg acttggtcac agtgaaagcc tgatggtgaa
    21841 acaataaaga ctgcatcgat cacggaactt ttgaccattt cttcatagct ggaaaaagta
    21901 tgcttgacgc ctaatttctt ctgtgcgaat gctaattctg tttcaactaa actgcacgca
    21961 gccgtaagct cacaacaact gacacctgtt gctaaatttt gtgcatgtac ttttcctaat
    22021 cttcctaaac cgacgatgcc tactctaatg ttgttcatga aatacctccg ttttctgaga
    22081 attactcatt gtatagtgct ggtcgttcat ccatttgaat cattactttt gtttttgttt
    22141 cacgagactc atgacaagcc atcatggtga tcgctgctac atacccatcc caagcggtag
    22201 gaccgacaac cgcatcattt ttgatcgaat cgatccacag cttgaattca taatcatagg
    22261 catccaaaaa gcgggtctgc caatctggtg aaacaggtgt gtaatttttt tcttccgatt
    22321 taattcgtgt ataggctggg tccgtcattc ctacttctgc cgtttcgaaa accacatcac
    22381 atttaatatc gtagccaaag cggcaattca caaagatttc caaatcaatc gttacaccgg
    22441 attttgtttg aaagatcagc agctgcggat catgcaaatc ggcatgggag ttgctggttt
    22501 ttttcggcaa gatcatttga acagattcat aatcttcatt tagcaaccag cgaagcacat
    22561 caatctcatg aatcgcggtg ttgctgatcg ccatcggtgt tgtgtagttt tcatctgcgc
    22621 taggatttct atgggcacaa tgcatcatta atgcttctcc gtattgcttc gattcgattg
    22681 cttctttcag ttcaatgtag ccgcggtcat agcggcgcat gaagccaacc tgaaccaatt
    22741 tcttcccata agcaacttcc gcatcaacga tccggcggca gcctgctgca tccatcgcta
    22801 atggcttttc gcagaaaaca tatttcccat gcttgattgc ttctaacaca tactgctcat
    22861 gggtcgggtc ccaagaagtc acaacaacga catccacatc ttcagaagcg atcaatgctt
    22921 ctccggtttc aaagaattta gcatcgacac gtttggcaac ctctttagca gcttttacat
    22981 tgatatctga gactgctgtc acataagccc cttgtgtttt ctgattgatc ctgtcgatat
    23041 gctcgatgcc gattgcacct gttccgataa cgcctacttt tactctcaat tacttatccc
    23101 cctgttctgt tgtttaagca cacatagtat gcgcttacaa aattattcct gtgtagcttt
    23161 tattcttaaa aggtagagcc ctctctttgc tccgcctttt actacccata agcttgtacg
    23221 tataattcct taatttgact atgactaggc acctttttat tattttgcgg acttccgctg
    23281 gtcaacgcat ccgcggccat tttgtctaag gctgccataa acgcttgctg atcaatacca
    23341 taatctcgaa tcgttgggat cgacagctgc tcacagagct cttggatttt tttaatcaga
    23401 taatccgcag cctcttccag cgataaatct tcggctgccg gataaataaa tctggcgatc
    23461 gtcgctaact cagtctgaat agcatccttt gagaattcaa aaaagaccgg caataacatc
    23521 gcatttgata tcccatgagg aatgtgaaaa agtgccccca ccggtcgact cattccatga
    23581 atcaatgtga ctgacgcatt cgaaaaggca atccccgcct ccgttgaagc taagctcatt
    23641 tgttctctgg cttcagtgtc gtcgctattt tcatatgcct gagaaagcgt gttcaaaatt
    23701 ttcttcatcg cttccagtga aaatagttgc gttacttcat tggctttgac tgaaacataa
    23761 gattccaaag cgtgacatag cgcatcgact cccgttgccg cggttacttt tggtggtaca
    23821 gttttcgtaa aatcagagtc aacgattgag atctgtggca tgaaaacgag atccttcatc
    23881 atcattttga ctgctgtttt tgtattggta ataactgcca catcggttac ttccgaacca
    23941 gttccagcag ttgttgacac tgcaatcagc ggaatgcccg cttgcagctg atcaaaattt
    24001 cttgcatgaa tcgcttcgag acttaattga ttagcagcca aaacggctac caccttggct
    24061 gtatccagac agctgccgcc accaaaagca atgatatgat cacattgatg cttctgaaaa
    24121 agcttcagcg catcataaac attttcttgc gtcggttcgg cttgaatgtc tgtatactcc
    24181 gctaattgat catctgtttt tcccaacagc tccttgatct tttgaagatg cccggtctgt
    24241 tccataaccg tatcactaat gattaacggc ttgttgccta gttgttctga ttcttccata
    24301 agaaattgga ttgttccgcc gccaattctt aaattggcag gcattttcag ttgaatattc
    24361 acagaaaaaa cctctctctg gcttatagcc tataacagaa tcctcgaagc tctttaacag
    24421 agcttcgaag caaatcttta aatctcactt tgcgcataac gtgctgtaac aactttccga
    24481 tgggtataaa actcaacact gtctttgcca ttggcatgca aatcaccaaa gaaagagtgc
    24541 ttccagcctg aaaaggcaaa ccaagccatc ggtgcaggca ccccaagatt gacccccagc
    24601 atcccagcat caacattttc tctgaagtag cgaatcgctg atgcgttgtt ggtatacaga
    24661 cacgaaccat tcgcaaattc agattcattc gtcagctcaa cggctgccat caaatctttt
    24721 acccgcacga ttgatgcgac cggggcaaaa atctcatcct gccagatctt catatccatc
    24781 gtgacattat caaaaatcgt tggtccaaca aaatagcctt tttctggaat gttttcgcga
    24841 ccatctagta ccagtttcgc tccttcatcg accccttttt ggatatagtc aaaggtccgc
    24901 tgctgtgcat cttttcgaat cactggtcct aaaaagaccc cttcgtccaa gccattgccg
    24961 atcttgatcg cttttgccgc agccttaaat ttttcaacaa actgatccgc gatttcctct
    25021 tccaaaacaa tgacggaggc tgccatgcag cgttcaccag cagaaccaaa agctccgcca
    25081 ataaggtcct tgactgcccg atcaatattg gcatctttca gcacaacaac atgatttttc
    25141 gctccagtca acgcttgtac ccgttttaga ttttttgttc cttcacggta aacgtattca
    25201 ccgacatttt ttgatcctac aaagctgatg ccttttacca gtggatcttg cagcaatgtg
    25261 ttgacaacat cgtgggcacc atagacaaca ttaaaaacac cgctaggaaa gcctgctttt
    25321 tcaaccagct caacaatttt ctccatcaac aatggtgttt tttctgaagg cttcagcacc
    25381 accgtatttc ctgccgccag agccatcggg aagatccaga agggcaccat cattggaaaa
    25441 ttaaacggtg tgatccctcc gattactccg attgggtaac gataattcgt gatctcaata
    25501 tcagtcgccg ctgttgataa agaatctccc atcattaaat tggtgattcc cgctgcgtgc
    25561 tccacatttt cgattcctcg ttgaacttct cccagtgact cggacaatga tttcccattt
    25621 tccagcgtga tcaaacgcgc caattcactt ttatgatctg ttaaaagctg atgcagcttg
    25681 aacagataac gtccgcgaac aggaacagga acttttttcc aagtttgaaa cgcagcatcg
    25741 gcagctttaa ctgcttcggc tgtctctgct ttgctagaca acggtacatt cgccagcgct
    25801 tcttctgtag ccggattcat tacttcttca aactctgttg agcgactagc gacccaacga
    25861 ccatcaatat aatttcttaa aaatcttgtc attta
//
LOCUS       DACCWV010000026        25592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCWV010000026 DACCWV010000000
VERSION     DACCWV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.026"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            241..795
                     /locus_tag="IVW84_000961"
     CDS             241..795
                     /locus_tag="IVW84_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7917174.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(968..1441)
                     /locus_tag="IVW84_000962"
     CDS             complement(968..1441)
                     /locus_tag="IVW84_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7917175.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1453..1989
                     /locus_tag="IVW84_000963"
     CDS             1453..1989
                     /locus_tag="IVW84_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7917176.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2029..2400
                     /locus_tag="IVW84_000964"
     CDS             2029..2400
                     /locus_tag="IVW84_000964"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7917177.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2684..3955
                     /locus_tag="IVW84_000965"
     CDS             2684..3955
                     /locus_tag="IVW84_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7917178.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4200..5225
                     /locus_tag="IVW84_000966"
     CDS             4200..5225
                     /locus_tag="IVW84_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917179.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5519..6628
                     /locus_tag="IVW84_000967"
     CDS             5519..6628
                     /locus_tag="IVW84_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7917180.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6711..7607
                     /locus_tag="IVW84_000968"
                     /pseudo
     CDS             6711..7607
                     /locus_tag="IVW84_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7623..8447
                     /locus_tag="IVW84_000969"
     CDS             7623..8447
                     /locus_tag="IVW84_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917181.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8479..9789
                     /locus_tag="IVW84_000970"
     CDS             8479..9789
                     /locus_tag="IVW84_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7917182.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9937..11196
                     /locus_tag="IVW84_000971"
     CDS             9937..11196
                     /locus_tag="IVW84_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7917183.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11206..12078
                     /locus_tag="IVW84_000972"
     CDS             11206..12078
                     /locus_tag="IVW84_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917184.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYCLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12090..12920
                     /locus_tag="IVW84_000973"
     CDS             12090..12920
                     /locus_tag="IVW84_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917185.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12960..15143
                     /locus_tag="IVW84_000974"
     CDS             12960..15143
                     /locus_tag="IVW84_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7917186.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15204..16424
                     /locus_tag="IVW84_000975"
     CDS             15204..16424
                     /locus_tag="IVW84_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7917187.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16421..17881
                     /gene="gtfA"
                     /locus_tag="IVW84_000976"
     CDS             16421..17881
                     /gene="gtfA"
                     /locus_tag="IVW84_000976"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7917188.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17958..19064
                     /locus_tag="IVW84_000977"
                     /pseudo
     CDS             17958..19064
                     /locus_tag="IVW84_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19386..19595
                     /locus_tag="IVW84_000978"
     CDS             19386..19595
                     /locus_tag="IVW84_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7917189.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19588..19737
                     /locus_tag="IVW84_000979"
     CDS             19588..19737
                     /locus_tag="IVW84_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7917190.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19772..20556
                     /locus_tag="IVW84_000980"
                     /pseudo
     CDS             19772..20556
                     /locus_tag="IVW84_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20840..21925)
                     /gene="xerS"
                     /locus_tag="IVW84_000981"
     CDS             complement(20840..21925)
                     /gene="xerS"
                     /locus_tag="IVW84_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7917191.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22259..22492
                     /locus_tag="IVW84_000982"
     CDS             22259..22492
                     /locus_tag="IVW84_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7917192.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22489..22896
                     /locus_tag="IVW84_000983"
     CDS             22489..22896
                     /locus_tag="IVW84_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7917193.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24033..24551)
                     /locus_tag="IVW84_000984"
     CDS             complement(24033..24551)
                     /locus_tag="IVW84_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7917194.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8713 a         3911 c         4768 g         8200 t
ORIGIN      
        1 aacttctacg gataactgcc cgtctaaaat ttaaataaga gcttagaatc ttcatataag
       61 taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa
      121 atcataaact aaatcccttg attttattga ctttcaaaag tttatatata taagttaatg
      181 attatggtaa aatatataca taatcattaa ctcagaatat ttttaagaag ggaaacaata
      241 gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg
      301 ctaagaagaa caagtaatcc ggaaagagat agttttttat ttttgtttgg aatcaataat
      361 gggttacgta tgtcagatat tataggatta aaagttggag atatacgagg caaaagtaag
      421 cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga
      481 gaagaaattt tattatatac tgaaggtaaa gaagaaaatg attggttatt tccgagtcgg
      541 caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa
      601 aaattaggac gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac
      661 tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca
      721 attacaaaaa ggtacattgg tataactgaa gatgaaatag gagcatcact tcgtggattt
      781 aaactaggtg tataatttaa aaatttttat aaacagtaaa gacaaagatt aaaagctatt
      841 tttatctaga acgattttat ttcataaagt tcgcggaata aaagctgggg ttactggtac
      901 ttggtatagg ttcgtgttat ataaactaag taattaaata acatttttag gttggaaaag
      961 gatgaaatta aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg cggaataaga
     1021 aaaatactga taactcaacg tttagcagac tatctaatag ggatagtctg cttttttcgt
     1081 ttttgaccat acttttgacc ttctgcaaac ttccttgctt ttcctctgta gaactcatga
     1141 aatctgcaaa acgaattgcc gtttcctctg tctgcttttt cgcgatatgg gtgtatatat
     1201 catccatagt ggttttaata tctgtatgtc caagtcgaac atacacttct ttgacagacg
     1261 caccagattc aaagagcaga ctacaatggg tatgacgaaa accatgaagt gtgatatgtt
     1321 ttaagttatt ctctttaatg atgattttca aattgtgatt tacatagtct aaataaaggg
     1381 gcttattttc ttctgatgaa aacaaaagtt gttcaccatc ttttataggt ttgattctca
     1441 tttttagata ttatgcccgc acgtattcat cacatttcta ttgtgaatcg ctttattcga
     1501 ccgacatttg acttttatca taatatactc ggtttaaaat tactaatgaa aacaattaat
     1561 caagacgatc ataccatgta tcacctgttt ttttcagata atcaccaccg tgtgggaact
     1621 gaactgactt tttttgaagt acaagaaggg aacaaccagt tttttggaac caatacgatt
     1681 gaacgaacga tactaaaagt tccttccgaa gcatcattgc atttttggga aatatatttt
     1741 gaaacacaag gcgtttgcca ttatggaatc gaatcatttt ctggacgacc tatcttacgt
     1801 tttgaagggc cagatgcaac acgattagct ttagttccct tacgcgaatt tgaagatatt
     1861 gatgattatt tcccgtatgt cacagaccag atccctacag agcatgccat tttaggaatt
     1921 gatgccattc aactacgcgt acaatattcc gatgcgactg aacgagagtt attgtcggta
     1981 ggctggtagc acaaagaata cgtcagtttc ttttcgggtc atcaacgagt gacaatctta
     2041 gaaaatcaag acactcagtt ctatcaagaa gttcagatta ttcaagatcg tgagaatcca
     2101 gttgccattg aagggattgg tagcgtccat catgttgcgt ttggcgtaga aaataaatct
     2161 gatttacaac gcatcgacaa acaattacaa gagcgcaatt ttataaattc aggaataaaa
     2221 gatcgtgaat tttttgtatc actttattat cgtgacccaa atcaattatt gattgaaatt
     2281 gctacagggg aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc acgctttgaa
     2341 gaaattcctt tatttttacc gcagtactta aatttcattc gtgaacaagt ggagcaataa
     2401 ttagctaatc aaatccatga cgaagagtaa gaggttgact cataagtcaa aaaataaaga
     2461 aaaacgcatc aaatccattc taaagaatgt tgatttgatg cggttcctat ttttataaac
     2521 tatcattttc gactaataaa actacttatg agtcagcctc tctttttttc ttgattttta
     2581 tacctctttc taggtaaact aaacatctgc caaaaatacc gtgtctcatt gaaacattct
     2641 caactatttc aaaataaagg ggagtaccaa atgaacgagt atattgaaat tatccacgca
     2701 agagaaacaa cttaaaaatg tcagtctcaa aattcccaaa aataaaatta gtatttttac
     2761 cagcgtatct ggttcaggaa aatcatcgat tgtttttgat actattaccc aagaagctgg
     2821 tcgtcaactg aatgacacct atgatagttt tacccgttta tttttaccta aatacagtcg
     2881 tccagaggtt gatgagattg ataatctttc tacggcgatt gtggtcaatc aaaagaagat
     2941 tggtggcagt gcgcgctcaa cgttagggac gattactaac atcaatgcgt tatttcgtgt
     3001 gttgttttca tgttttgcaa cacctagttt aggatatgca aatgcttatt catttaatga
     3061 tcctagtgga atgtgttctg tctgtcaggg aatcggtaag acgattgcat ttttaatagg
     3121 attgctttat ttttcggcta ttgataatgc tgattttgta ttttctgatc catttaccgc
     3181 agaagttttg tctggaattg aggaacaggc aaagaatcat ggttattttg tattggtgca
     3241 taatgttaat tcagcagaag atgttaaaac tattcagaga aattggcgtt ttgaggggtt
     3301 cattgttgtt ggtgtacgtg taaaagaatt tttggaatta aataacgttt ttgatggtcc
     3361 tgttgtttat attgatacgc atctttcaaa agagacatta agagaacttg aacaacaaat
     3421 gaatcgttta tttgtaaata caaatgatta ccaagctagt acattggcag tggaacattt
     3481 gattcattat ggtcatgaga aaatagcgtt tctttctttt gactacattg taggtgaacc
     3541 aagtgttatt caagaacgac atcaagctta tatcgataca ttaaagaaat atcatttaac
     3601 ctttaaatcg cattggatat ataacaactc tgaatttgag cgaatttatc aagaattagc
     3661 tgaatttact gcaattgttg ttactggaga ttatttagcg gctaaattag taaaattttt
     3721 aaaaaataag aataaacgag tgatggaaca attatcgatt attggatttg atgatattac
     3781 ttttgcagaa ttaatggatc ctgcgttgac aacaatacga ctaaatccag gtaacaaggg
     3841 ggcgattgct tttcaccaac tgattggact ggccaaacaa gaagaaataa aaaatcaagt
     3901 actactatta gagggagaat tgatttctcg agactctgtt cacaaaatca aataaaaaag
     3961 ctctgtgaaa taagtatagt taacaataaa atatccgaac gatagagaga atgaactgtg
     4021 atctagcagt tcctcgtcgc aaattcacta gaattagcat tatttactct aaattgttcg
     4081 gatatttttt cttgttttta attatgatta cagtgcctat ttttaacgaa atttgcttat
     4141 tttcaaaatg tatgattata tgttagtaaa cactttcaaa tatgaaaaga ggagctatta
     4201 tggtaggaat tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca gtatcgcgcg
     4261 tgctaaatga ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag attgcagaat
     4321 cggttaattt ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta acaaatattg
     4381 cattaataac gacagtttct gaagtagatg agttggaaga tccctatttt cgaacgattc
     4441 gcagagggat tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa attattcgtt
     4501 tgtcagaaac accgttggag caagacgatt tgctaaaatg tcaaggtgtg ttgattattg
     4561 ggcaggtgct atcttctgtg attgaagaag tatcagcaat taattcgaat gtcgtagtga
     4621 ttgatgaccc tagtattgaa gctgaagtag atgcagttta tactgattta acaaaagcaa
     4681 cgaatcagca tttagatcga ttgtataaaa aaggacaccg aaacattgcg tttatcggag
     4741 gaggacgtgt tcgtttagat agccaaggaa ataaacacgt gtcagatgat gattatcgaa
     4801 aaatagccta tgagacgtgg atgaaacaaa aaggattaga aaaatatagt caaatttatt
     4861 tagaaggctg gaccacactt gacggaatgc aggcagcaga tttacttttg aaaaaacatg
     4921 ctaaagataa gttgccgact gctatcatgg taggaaatga tcctattgct gtgggggttt
     4981 atcgttcact tcaaaaaaat ggtctacata ttccagaaga tatttcgatt gtgagttttg
     5041 ataatattga agttgctgaa tttttaacac ctgcattaaa tacggtgaat gtgaatacag
     5101 aagaaattgg aaaactagca gttcgcatgg ttaatgaacg aataaaagaa gttagaaatg
     5161 tttcgatcaa agtgattgtt tctaataata ttattgttag agaaagtgaa aaaaatatcg
     5221 attaaaaaag taaacatcca aaaaagggtg tttacttttt tgtttactaa tatttttagt
     5281 aaaaaatctc gacaaaacat taagtaaaca gttatggtgt tgttaacaaa aagaaaacgc
     5341 attcaaaaca ggaggaatta agatgaaaca tttcaaaaaa atagctttaa cagggatagc
     5401 tgttttaact gggacattac tcgcagcatg tggtggggga gatagtaatt ctaaagacgg
     5461 gcaagtaact ttaagctatg cgagttgaga tactgaccaa gctccaggac taagaaaaat
     5521 gttagatgaa tttgaaaaac aaaatcctga tatcaaagta gaaatggaga caacgccttg
     5581 ggatcaatat tgggttaaat tagaagctgc aacaacggga ggtaatatgc cagatgtagt
     5641 aaccatgcac tctagcgaat cctataaata tatgtcacag aatgctttga tgggacttga
     5701 tgatgttatc aaagaaaatg atattgacat gggaaacttt actgaaggaa ttgctgattt
     5761 ctatacgctt gaagacgaac tatacgcaat ccctaaagat gcttctgtag tggggctatg
     5821 gtacaacaaa gaattatttg ataatgcagg tgtcgattat ccagatgaaa catggacatg
     5881 ggaaacactc agacaagctg caattgattt aacagatgaa tcaaaaggca tctatggttt
     5941 tgcatcacat attggaacag agactggcta ctggccattt atttatcaaa atgggggaga
     6001 agtatttgcc gatgacaata caaaatcagg tttgaatacc ccagaagcac aagaagcttt
     6061 agctttttat cgagatattg cacttgttga tcatgcttca ccaacaatta cagaattaca
     6121 agatcctgat aagttaagtc gttttcaatc aggtagatta gcaatgatga ttgaaggaaa
     6181 ctggtttgca ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg cttctgccgt
     6241 gttacctaag ggaaaagtta atgcaacaat tacaaatgga cttggttggg ctgccagtgc
     6301 gagtacaaaa tacccagaag aagtgaaaaa actaatggca tttttagcta gtgaggacgc
     6361 caataaaatc caagcggaaa cgggggcatc aatcccagca ctaaaaggct ttgaagacga
     6421 gtgggctgct tcttcagata caattgattt gagtgctttt ggaaaaatgt tggagtatgg
     6481 agtccctcgt ccatacaata aagatggatt acgagctgaa caagcagaag ttcaaattat
     6541 taaccgtatt ctgtctggtg aagtaactgt tgaagatgga accgctcaaa ttgaaaaaga
     6601 agtcaatgcg atattgagcg gaaattaaat tagttaatta tacaatatac aggcgaaagg
     6661 attctatttc gtctgtatat taatggatga ataccaaaaa gggggatatt atgaaaaatc
     6721 gaatttttgg aaaatatcgt aaagatcaag tgatatgggg ctatattttt atttttccaa
     6781 cggcattagg attaatttta ttaaatatat ggccagcaat tcaaacagca attttaagtt
     6841 ttcaaaaaac ggtagggttc ggtgatactg aatgggtcgg attaagaaat taccaaaaaa
     6901 tgtttgcaga cggtgaagtt tttcagtctt tattaaacac attaatttat gcagtggttt
     6961 cgatacctag tattgttgta ttatccttgt tggtggcagt tttaatgaat agaaaagtta
     7021 aaggattgtc gatttatcgg acaatttatt ttctaccagt cgttgctgca ccatccgcag
     7081 tcgcaatgat ttggcgctgg atgttcaata atgattatgg actgattaat aacatgttgg
     7141 ctaaaatagg tctcgatgga ccagcatggt taacggatcc atcgattgcg atttattcta
     7201 ttatcattgt aggagtttgg agtgctattg gttataacat ggtgttatta ctagccggct
     7261 tataagaaat tcctaaagat tattatgaag cagcggaaat tgatggtgct agtccaatcc
     7321 agcagttttt caacattacg ttaccattag ttacaccaac attatttttt gtagttgtaa
     7381 caacagttat caatgctttc caagtatttg atgttatttt tatgatgatt actcctaata
     7441 gtaccgccat gtttaaatca caatcattag cctatttatt ctataaacat tcatttattt
     7501 taaatgacaa gggatatggt tcggcaatcg ttatgttcct tttagtgatt atattaatta
     7561 ttactgcaat tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga ggctatcaaa
     7621 ttatgaaaaa taaatttttt tcaaatgcgc caatccatat catgcttatt ttaggtggta
     7681 ttgcaatggc attacctttt atttggatga ttttaacttc agggaaaacg ttggttgaat
     7741 ctacccaaat tccacctaca attttccctg agaaatttca gctaagtaac tatcaacaag
     7801 tacttgattt attaccattc atggcttttt atatcaatac atttttaatg gttgttggac
     7861 gagtgattgg ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg cgtctaagtt
     7921 ttccagggaa aaatatcctt ttcagcttag tacttattca aatgatgatt cccggttcca
     7981 tttttattac tccacagtat ttattagcac aaaaattggg tattttaaat acaattccag
     8041 cattaatatt accaggggta gttagtgcgt ttggtacgtt tttattaaga caatttttct
     8101 taggtttacc tgatgaattg gaggaagcgg cctatttaga aggtgccaat atttggacga
     8161 tctttttcaa aattatgttg ccgcttgcta aatcaggatt ggttgcactt ggaatattca
     8221 cagcgttgtt tgcatttaaa gatttaatgt ggccattgat tgtcaatact tcaattgata
     8281 agatgccttt agctccggga ttagcaaact tacaaggaca atatgccacg aacttcccac
     8341 aattaatggc aggttcgtta ttagcaattt ggccaatagt tgtgatattc atcgttttcc
     8401 aaagaaaatt cattgaagga attgctacaa caggtagtaa attataaaac tattaattag
     8461 tgattggaga gtaagtacat gacaaaaatt acatttattg gtgcaggaag tactattttt
     8521 gcaaaaaacg tattaggcga tgcgatgtta actccaagtc tacaagatgc acatattgca
     8581 ttgtacgata ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa aaatattaat
     8641 aaaaattcga atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa agaagcgtta
     8701 agagaggctg attttgttat caatgctatt caagtgggtg gttataaacc ttcaacagtg
     8761 attgattttg atattcctaa aaaatatggg ttgcgacaaa ctattggtga caccatgggg
     8821 attggtggga tttttagaac cctacggaca ctaccagtaa tgttagactt tgctaaagaa
     8881 atggaagaag tttgtccaaa tgcttggtta ttgaactata caaatccgat ggcgatgtta
     8941 accatgggtg ttttaaaagc gacgagtatt aagactgttg gtttatgtca tagcgttcaa
     9001 gtatgcgtac cagaattgtt tgagcattta ggaatagccg atcaatatga cttaaatgac
     9061 tttcaatgga aaattgcagg cattaatcat atggcattct tgttggaaat tacaaaaaat
     9121 ggggaagatt tctatccaac aattcgtcag ttagctgcag aaaaaccaaa tccacatcca
     9181 gattctgtac gttttgaatt gttgaaacgt tttggttact atattacaga atcaagtgaa
     9241 cacaatgcag aataccatcc atactttatt aaatcaaaat acccagaatt gattgacgaa
     9301 ttagggattc cgttggatga atacttacgt cgttgtgaac aacaaattaa tgattgggaa
     9361 agcatgcgag aggacattgt aaataatggg gagttaacac atacgagaag ccgtgaatat
     9421 gcttcatata tcatggattc tattacgaca ggaactccgt cagtgattgc aggaaatgtc
     9481 ttaaataaag gcttaatcac aaacttaccg acagatgttt gtgtggaagt accttgttta
     9541 gtcgataaaa atggcgtgca accaacctat gtcggtgaat tgccaacaca attagctgct
     9601 ttaaatcgga cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt aacactagat
     9661 aaatcaaaaa tttatcaagc agcgtattta gatccacatc tatcatctga attgtcttta
     9721 acagatatca aaaatttagt ggatgattta atcgaagcac atggggacta tttacctgaa
     9781 tataattaaa aagaagctga aagagtaagg aattttcaat tccttactct tttcttagtt
     9841 aaatttagta aaaatattga caaaacactt agtaaacagt tacattagtg ttaacaaaaa
     9901 gaaaacgcat tcaaattgta taaggaggag cataaaatga agtggtctaa aaaaattatt
     9961 ttagcttgta tagcactagg agcaacgatc tctttgactg cttgtggaaa aaatcaggaa
    10021 gatgcaagcg gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat gactcaaacg
    10081 attcaggaga ttgctaaaga ttttgaagca aaaaaccctg atattcatgt aaaagttgtg
    10141 gatgtaccga atgctggtga agtaattaaa acacgtatgc ttgcaggcga tgtaccagat
    10201 gtcattaatc tttatcctca aagtatagaa ttgaaagaat gggcgaaagc tggttattta
    10261 gaggacttaa ccgaagaacc gtatttagag aatatcaaaa atggatatgc acaacgtttt
    10321 gctattgagg atcgtgtata tagcatacca ttaaccgcta atgtctatgg tttttactat
    10381 aacaaaactg cttttgagga aatggggatt caagcaccag aaacttgggc agaatttgaa
    10441 aaaatagtag ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc aggagcagaa
    10501 ggctggactt taaatggtta tcaccaacta gctatagcga ctgctgctgg tggtgaagag
    10561 gaagcgaaca atgtttggcg tttctcagaa gttaatggaa ttaatgctga aagcaaagaa
    10621 atgcaaaaag attttaatcg attagatttg ttaagagaac caaaagcttt gcaaaacaac
    10681 tggcaaggtg ccggttataa cgatacagta gttactttta caaaaggtga tgcgttgatt
    10741 atgccgaatg gttcatgggc aatgccgatg attcattcac aaaatccaga ttttgaagta
    10801 ggaacttttc cttttccagc agatgaagca ggtcaaagtc taacgattgg cgcaggagac
    10861 ctagccttat cagtttctgc aacttctcaa cataaagaag aagccaaacg ttttgtagaa
    10921 tatatgacaa cacctgaagc gatgcagaaa tattatgatg tagatggtgc accgtgtgct
    10981 gttgagggcg ttatcgagaa tactcaggat tcaccattaa gtggtttaac tgaacttgct
    11041 tttactgatc gtcatttagt ttggttagcc aaagattgga atagtgaaaa tgatttttat
    11101 accttaacaa caaattattt acacaatggc aatcaaaaag ccatgattaa cgcattaaat
    11161 gctttcttta atccaatgaa agctgacgga aggtaggaaa ataaaatgaa aataaaagaa
    11221 attttaaatc gttactgggc aatcattttt gtagcaattc caattatttt gcagatgatt
    11281 tttttctact ttccaatggt tcaaggtgct ttttacagtt taactaattg gactggtctg
    11341 acttataatt ttgattttgt gggtttgaac aattataaga ttcttttttc tgatccgaag
    11401 tttatcaaat cgattggctt cactttaatt gtgacgcttt gcatgattgc tggacaaatt
    11461 ggttttggta ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa aacattcttt
    11521 agagcatggt tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt agtatttaaa
    11581 caaattttta actattgttt accaaaaatt ggtagtatgt taggcattga atttttacaa
    11641 accagtcttt taggaacaac atggggagcg atttttgcag taatttttgt tttactttgg
    11701 caaggaattg ccatgccagt catcattttc ttagcgggtt ttcaaagtat tccacaagaa
    11761 attattgaag ctgctagaat ggatggggcg aatgataagc aaattttttg gaatattgaa
    11821 atcacctact tattaccaag tatttcaatg gtatttatta tggcattaaa aggtggctta
    11881 acagcatttg atcaaatttt tgctttaact ggtggtggac caagcgatgc cacaacatct
    11941 ttggggttac ttgtttataa ttatgctttt aaaaacaatc agtttggtta tgcgaatgct
    12001 attgctttag tcttatttat cttaattggg attgtttcac tggcacaatt aaaattatcg
    12061 aaaaaatttg aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa aatatttcta
    12121 aatacttgct tttattatta gggggcttca tgattttagt tcccttagtt tcaacgctat
    12181 tcggttcatt taaaacaaca cccgatatta tgaataattt ctttcgtttt cctaatccag
    12241 cgactttgga aaatttccga agattgttag cggatggaat tggtcattac ttttggaatt
    12301 cggcaatcat cacaatcatt gcagtagttg ctgtgacagc aattattcct atggctgctt
    12361 attcactagc aagaaaaatg actcgtggga aagcatacgg attgatgtat ttatttttaa
    12421 ttttaggaat ttttgttcct ttccaagtaa ttatgattcc gattaccgtt atgatgagta
    12481 aaattggttt agcaaatatg tggggattaa tcttactcta tctcacttat gccgtaccac
    12541 agacactatt tttatatgtt ggttttatta aactaaatgt gcctatcagt ctggatgagg
    12601 ctgctgaaat tgatggggct agtcacttta caacttattt caaaattgtt ttcccaatgt
    12661 taaaaccaat gcatgcgacg actattattc ttaatgctct atggttttgg aatgatttca
    12721 tgctaccatt attaatttta aatcgtgatt ctaatatgtg gacactacca ttgtttcaat
    12781 ataattatac gggacaatat tttaacgact atggtccaag ttttgcttcc tatacagtag
    12841 gaattgtcac gattacacta gtttatcttg ctttccaaag aagtattata gctggaatga
    12901 gcaatggagc agtgaaataa ccaataaatt attctcatta taaaggagtt cataaactta
    12961 tgattatatt tgacaatcac acacaaacat ttcatttaca aaataaaaaa attagctatc
    13021 tattaggaat cgaagaaaat aattatttaa cgcatcttta ttttggaaaa aagattacgc
    13081 actattctgg tgggtatcgc tacccccgaa cagatcgttc cttctcaccc aacaatgcaa
    13141 atgcgcaaga tcgtttatta tctttagaca cattaccatt agaatttcca ggatatggac
    13201 atggtgattt tagagaaccg gcgcataata ttaaattagg aaacggtagt cgtgtaaatg
    13261 attttcgtta tgatagctac gaaattcaaa aaggaaagcc cagcttagcg ggattgccag
    13321 ccttatatac ggaagatgat gatgaagctg aaacgctgat tattcaattg aaagacgcga
    13381 ttagtggatt gactttacag ttgttctaca caatttatca aaatcacagt gtaattattc
    13441 gttctacgca attagtaaat aatagtgatg aaacagtgga gattaaccgc ttagctagtc
    13501 aatcaattga ttttccaaat cgtccactcg agttaattca tttaaatggt acctgggcaa
    13561 gagaacgtca aatgactaga gaaaaaattc atacagggat aaaagtatta gatagtaaac
    13621 gcggatcaag cagccatcaa caaaatccgt ttgttgcttt agtagatgcc aaaacagatg
    13681 aatttcaagg agaggcatat ggatttaatt tagtttatag cggtaatcac gaaacggtga
    13741 ttcaaaaaga tccatttgac caaacacggg tcattatggg aattaattca tttaattttg
    13801 gttggcaact aaaaactggc gactcatttc aaacgccaga agtcgtgatg gtttatagta
    13861 atgaaggtct gaatacgtta tcacagacgt atcatgaatt gtataatcac cgtttaattc
    13921 gtggaaacta tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa gcaacgtatt
    13981 ttgattttaa cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac ttgggcattg
    14041 aaatgtttgt tttagatgat ggttggtttg gcggtcgtga aaatgacttc acttcattag
    14101 gtgattgggt tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta gccaagcaag
    14161 tgcatgataa cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt tctgaagata
    14221 gtgaactatt tcgagcacat ccagattggg cattgggtat tccaggtcgt gaccgctcaa
    14281 ttagtcgaaa tcaatatgtt cttgattttt caagacaaga tgttcgtgat aatatctatc
    14341 aacaaatgac ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc
    14401 gaaatatgac agaagtctat tctgtcttgt tggactcaac gatgcaaggt gaagtggctc
    14461 atcgttatat gttagggtta tatgaatttt tagaaaagtt aacaacgaat tatccaacta
    14521 ttttatttga aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca
    14581 tgccacaaac ttggacgagc gacaataccg atgccgtggc tcgtctgaaa attcaatatg
    14641 gaaccagctt agtgtatcca atttcaagta tgggtgcgca tgtttcagca gtcccaaatc
    14701 atcaaacagg aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt
    14761 ttggttatga gctagatcta acgcaattaa cttctgaaga aaaagcagaa attaaagaac
    14821 aagttgcctt ttataaaaag catcgccaac ttttgcaata tggaactttt tatcgcttgg
    14881 aaagtccgtt tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac caaacagaag
    14941 ccatggtctt ttatttccga gtattagcag aagctgcgca tccattagtc acattgaaat
    15001 tagcaggatt agcagaagaa gcaacttatc aaatggaaga tgtaacattt gctggcgatg
    15061 aattgatgaa tctcggtttt tatattgact cagaactaca tggtgattat gcgacacaac
    15121 gcttttattt gaaacagata tagtagcttg aatttacaat ataagtgcaa caccaaaaga
    15181 gactcattcc aaacgaagaa aatatgtttt atttacacgc atttactaaa aagcagcctg
    15241 atttgaattg ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt aactattggt
    15301 tagataaagg actaggcgga tttagaatcg atgcgatatt aaacatcaaa aaaaaactag
    15361 agtatggcat ctttgaatct gatggagaag atgggttagc cttcattggg ccctggattc
    15421 ttaatcagtc gggcattgaa gtgtggttaa aagaaatgag agagcggaca tttaaaccac
    15481 ataatagtat gacggtagca gaagccgatg ttcctaatga acgtttagat gaatatattg
    15541 gtgaagatgg ctattattcg atggtttttg attttagtta tacggatatt gatgtacctg
    15601 aaactggtga atggtttaaa gatagccaat ggacatggac ggatatgcgt accaatattt
    15661 ttacgaatca attagttacg caagataaag gttggggcgc cttatattta gaaaaccacg
    15721 atcaaccacg ttcaattaat aaatatattc cagaagaatg gatcaatgat tatagtaaaa
    15781 aaatgttggc gacgcttttt atgttactaa gaggtacccc ttttatttat caaggacaag
    15841 aactaggaat gaccaacata gaaatggcat cattagaaga ttttgatgat gtagctacgc
    15901 attctcagta taaacgggca ttagaatatg gattggcacc agagcaagca ttgaaagctg
    15961 tcaataaacg aagtcgggat aattctcgca cacctatgca atggaatacg caaaaaaatg
    16021 ccagattctc aacttctgat aatgtatggt taaaagtaaa tcctaattat ccagaactca
    16081 atgcagaagc tcagttaacc aatcaacagt cggtattcaa ttattatcat caattaatta
    16141 aattaagaaa aaatagtgta tacaaagacg tgctaatcta cggacaattt ctaccagtga
    16201 aagataaaaa tgaacatatt ttgctttatg aacgccaatt agaagataaa atcgtattag
    16261 ttatcttaaa cgttacagca acagaacaag aatattatgt agatgaacag taccaacaag
    16321 tattaatcaa taattatgaa gatgttttat tagatcaaag tcgaaaattg cgtttacgac
    16381 cgtttgaaag tattgtatta gctaattatg gaggacaatt atgaaaatta aaaacgaagc
    16441 aatgctcatc acctatgcag atagtttagg aaaaaacatg ggcgaattaa aacaagtctt
    16501 agacaaacat ttacaaggtg ttattggtgg tgtacatctg ttgccttttt ttccatcgac
    16561 aggtgatcga ggctttgcgc cgagtgatta tacgacagtt gatccagctc ttggaacatg
    16621 ggaagatgtg gaagcattag gtgaagatta ttatttgatg tttgatttta tgattaatca
    16681 catttctcgt gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc agtcaccata
    16741 caaagaaatg tttatccgta ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga
    16801 tattgattta atctacaagc gtaaagacaa agcgccgttt caaacggtag aatttgcgga
    16861 tggtacgaca gaagaggttt ggaatacctt tggggaagaa caaattgatt tagatgtgac
    16921 caaagaagtt gtgaaagaat ttattcgaga aaccatcaaa gatatggcga accatggctg
    16981 ttctttaatt cgattagatg cgtttgctta tgcgattaaa aaattagata ccaatgattt
    17041 cttcgtggaa ccagatattt gggaactttt agacgaagtg cgtcaagaag cagcgaaata
    17101 tgacgtcgaa ttattaccag aaatccatga gcattattcc attcaaatga aaattgccaa
    17161 tcatgattat tatatctatg acttcgcctt gccaatggtc actctgtatt cactttatag
    17221 tggaaaatca gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc agtttacaac
    17281 attggatacg catgatggaa ttggtgttgt ggacgctcgt gatttattaa cagatgaaga
    17341 attggattac acctctgagg aattgtataa agtcggggcg aatgtgaaga aggtctattc
    17401 aagtgccaat tacaataatt tagatattta tcaaattaac agcacctatt atagtgcatt
    17461 aggcgataat gaccgtagct atctgttagc acgtgcgatt caagtatttg cgccagggat
    17521 tccacaaatt tattacgttg ggttattagc aggaaaaaat gatattgaac tgttggaagc
    17581 aacgaaagaa gggcgtaata ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga
    17641 agtgcaacgg ccagtgattc aacaattatt tgcactttta aaatttagaa atgaatctgc
    17701 ggcgtttgat ttggatggaa cgattgaagt agcaacgcca tcagaatcag aaatagtgat
    17761 tacacgtaaa aataaaaacg gtagtgatag tgcaacatta acggctaact tggatacgaa
    17821 agaatttatc attgagtcta aagatataaa aattttcact agtgttgcat taaaatttta
    17881 acctattcca tagaacaaaa aatcctttat actagcactt ggatgactta tccaagacca
    17941 ataaaaagga ctcatacatg gataagtata aaacaaatac agcatttaat aaatggtttt
    18001 ctgccataaa attaggaaaa ctttctgtca actcccaaaa atgtattttt gattacaatt
    18061 cgtatcgtaa aaaactgtct tttgaagctg tgctcaagct ctttctctac gcagttaatg
    18121 aagaaaaaga aagtttgcgt gatttgagta cagctcttat cgaccgatcg ctccaactag
    18181 aaaccgatgt gtcatcgatt agtcataccc aattgtctcg agcatttaat gcacttgaac
    18241 ctaaagtgtt agaagaaatt tttcaacaac tgttagaaaa agttcaacat caaatcaaac
    18301 caaccaagcg aaatcatctg tatctgattg attccagtac gttttcgctt agtttaaaac
    18361 gtcataagtg ggcgactttc cgtcaaacaa aatcaggtgt caagttacac ttaaactatt
    18421 gttatatgga cgatactacg atgtatccaa ctgattttac cttaacaaat gcccatgaac
    18481 atgatctcaa tcaactacca gtattggtga atcaaccaga ggcaacttac gtgtttgatc
    18541 gtgggtatta ggactttgaa aagatggatc agatgcattg ggatggctat ttcttcgtca
    18601 ctcggattaa aaaaaacacc aaagttcacg tgatagatac tctggaaaca tcgccagaaa
    18661 cagaaattct acgagacgag ctggttcgtt taggatctaa aacctatcta accgccaatt
    18721 tccgactagt cactgttcaa gataagaacg gacgtgtgtt tcaatttatc accaatcgaa
    18781 tggacgtctc ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa atcgaattgt
    18841 ttttcaagca catcaaacaa cacatgacca ttaaaacctt cttttctcaa agtgaaaaag
    18901 gcgtgcagaa tcaattgatt ttaacgatga tttccgctct cctcacgttt ctcatccaat
    18961 tggagacgaa aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt tatttactgt
    19021 ttcagccctt tgaatgttgt ctagaaaaat taattcccac atgagaaaaa agaaatttgg
    19081 ctcgttactt ttggttttca gttgaataac agataaaaaa ttttctggaa aaagtcatcc
    19141 tctgcgtcag cacagttttt tttgacttat tttcagagga aatagttttc agaatattct
    19201 aaaatgtttt atgcaacact agtgatattt tttagaatta gatttctaat gtgcattttt
    19261 tgagtattaa ggagcaagta tacaaagatt tataaagctg attaaatttg aataaaaata
    19321 tgcaggaacg ctactatttt tagtagtgtt cttttttata tttgaataaa aaatgtggtg
    19381 gttaaatggt ggttataaca aaaaatgaat taatgaaatt aggttatggg aagtatcagt
    19441 ctgagagaat catacgacag gcaaaagcaa ttatggtttc aaaaagctat acctattacg
    19501 aaaatagaag attaggacta gtacctgttg aatctgtaga agaagtttta ggtatgaaga
    19561 tagaggtaga ggaggatttt cagaatcatg gttaatttta aaaaagataa aaatgggaaa
    19621 tatcatttta ctgctagttt gggattcgac aaacgaacag ggaaaagagt acaaaaaaac
    19681 gtagaggggt taaaacagtt aaagaagaaa aaaagcgtat accgaacttg tttctaatta
    19741 tggaaaagaa gcagaaactt ctcaaagtac tatgttgttt gaggagtttt tttataaatt
    19801 attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc ggttatctac
    19861 aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg tccaattgac
    19921 attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt aagaaacatg
    19981 tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact tgaaaaaaat
    20041 cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa
    20101 gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca acattttggt
    20161 tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc cactgcatta
    20221 acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac actctattat
    20281 aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc
    20341 gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca agtttcaata
    20401 ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata tacaatccgt
    20461 catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat acacggatta
    20521 aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg aattaaagaa
    20581 aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt gtatctaaat
    20641 tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt ttctaaaatc
    20701 aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag gatcacacca
    20761 aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt tcttatttcc
    20821 gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc gcattgtcta
    20881 tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg gcagttaata
    20941 ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt ttgtgagggc
    21001 tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt gcaataccgg
    21061 atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg ttttctgtta
    21121 ctttatatcg ttctttccga atagctagat aattagctaa atattccatt gcgaatgctg
    21181 ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta ttcttatcta
    21241 aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca gatcctaaaa
    21301 tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc aaagctcgtt
    21361 tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt gtatccttat
    21421 ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt ttatctgtga
    21481 tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga ggattattgg
    21541 aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta ttgatagtag
    21601 aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc tcttttttta
    21661 cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca taggcagtta
    21721 actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga agccattcat
    21781 aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga tctttatcct
    21841 cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt gtatctattt
    21901 tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg ggagaagagt
    21961 atctctataa ttggactata ccaattatag aatgttttgt caattggtat gctctaaaat
    22021 ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat ttaaaataaa
    22081 tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt gaattttctt
    22141 aattaattat atagaaagaa caatctttct atagagttag acatgagagg gactatgctt
    22201 gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg agaagactat
    22261 ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa ctatttcaaa
    22321 ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag atgaagaaaa
    22381 acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt tagcaatatc
    22441 tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat gagtattcat
    22501 tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa atactctcct
    22561 aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga
    22621 gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc tatcctactt
    22681 atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc tttcttagct
    22741 acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa agatgctgta
    22801 aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa atatgaagtg
    22861 atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg tgtctttata
    22921 gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta taattcaaat
    22981 ccaactatat agaaaacgta aattttctat atagttggat taagataacg actatttatt
    23041 ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat aatcgcaaat
    23101 ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt tatttgttct
    23161 tttattaaat acacctacaa cttataaatt gatggtattt agataacacg aaagaataaa
    23221 aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt gagttagtgc
    23281 gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt gcagaaatcg
    23341 ttactgggat taaactaagc aataaaaact aatgttcccc tcattctttg aacttctact
    23401 atttgattta tttattctga taaatgacga tttcaccttc aggatcttca aatcccgtat
    23461 agaatttatt taagatctct tcagtatcag taataggata ggcgataggc cacataccct
    23521 ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag catattaaca
    23581 aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat ttcaattctt
    23641 cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat gtgggttgtt
    23701 gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa catcccaaaa
    23761 ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata tggcaccata
    23821 atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat tgatacaatc
    23881 taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc aataaaaacc
    23941 ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac aagaggtctc
    24001 ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt ccattttctt
    24061 atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa tttatatact
    24121 tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc agtgaataga
    24181 tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg attgagaatt
    24241 tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt atataactct
    24301 cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc atttctttca
    24361 tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa attttcagca
    24421 ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat agtttcaaat
    24481 tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc tttattttta
    24541 tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt catctataat
    24601 ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt gactgtctaa
    24661 ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc
    24721 agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac tggtttgcat
    24781 atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta ataaaaacta
    24841 ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca caaaatccat
    24901 tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta cagcatcgct
    24961 ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac gatgacacgt
    25021 tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt
    25081 taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt aaaggtcaag
    25141 atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca caatgcattt
    25201 ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta atgaaacaga
    25261 tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat tatgttaatc
    25321 ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg tttaaggttg
    25381 gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt aagttggttg
    25441 acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga cagtacctaa
    25501 aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc tcatttcaac
    25561 ttatatgttt tagtgaacta cccccactta gc
//
LOCUS       DACCWV010000027        24357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCWV010000027 DACCWV010000000
VERSION     DACCWV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.027"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..849)
                     /locus_tag="IVW84_000985"
     CDS             complement(1..849)
                     /locus_tag="IVW84_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7917195.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1073..3013)
                     /gene="thrS"
                     /locus_tag="IVW84_000986"
     CDS             complement(1073..3013)
                     /gene="thrS"
                     /locus_tag="IVW84_000986"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7917196.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3350..3529)
                     /locus_tag="IVW84_000987"
     CDS             complement(3350..3529)
                     /locus_tag="IVW84_000987"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7917197.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3576..5399)
                     /gene="cadA"
                     /locus_tag="IVW84_000988"
     CDS             complement(3576..5399)
                     /gene="cadA"
                     /locus_tag="IVW84_000988"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7917198.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5677..7956
                     /locus_tag="IVW84_000989"
     CDS             5677..7956
                     /locus_tag="IVW84_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7917199.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8049..8696)
                     /locus_tag="IVW84_000990"
     CDS             complement(8049..8696)
                     /locus_tag="IVW84_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917200.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8875..9594)
                     /locus_tag="IVW84_000991"
     CDS             complement(8875..9594)
                     /locus_tag="IVW84_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7917201.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9725..10876)
                     /locus_tag="IVW84_000992"
     CDS             complement(9725..10876)
                     /locus_tag="IVW84_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7917202.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11093..11833)
                     /locus_tag="IVW84_000993"
     CDS             complement(11093..11833)
                     /locus_tag="IVW84_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7917203.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11837..12160)
                     /gene="rsfS"
                     /locus_tag="IVW84_000994"
     CDS             complement(11837..12160)
                     /gene="rsfS"
                     /locus_tag="IVW84_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7917204.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12217..12810)
                     /locus_tag="IVW84_000995"
     CDS             complement(12217..12810)
                     /locus_tag="IVW84_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7917205.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12794..13441)
                     /locus_tag="IVW84_000996"
     CDS             complement(12794..13441)
                     /locus_tag="IVW84_000996"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7917206.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13454..13765)
                     /gene="yhbY"
                     /locus_tag="IVW84_000997"
     CDS             complement(13454..13765)
                     /gene="yhbY"
                     /locus_tag="IVW84_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7917207.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13781..14890)
                     /gene="yqeH"
                     /locus_tag="IVW84_000998"
     CDS             complement(13781..14890)
                     /gene="yqeH"
                     /locus_tag="IVW84_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7917208.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14887..15414)
                     /locus_tag="IVW84_000999"
     CDS             complement(14887..15414)
                     /locus_tag="IVW84_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7917209.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15594..16379)
                     /locus_tag="IVW84_001000"
     CDS             complement(15594..16379)
                     /locus_tag="IVW84_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7917210.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16372..17241)
                     /locus_tag="IVW84_001001"
     CDS             complement(16372..17241)
                     /locus_tag="IVW84_001001"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7917211.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17242..18621)
                     /locus_tag="IVW84_001002"
     CDS             complement(17242..18621)
                     /locus_tag="IVW84_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7917212.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18629..19048)
                     /gene="fabZ"
                     /locus_tag="IVW84_001003"
     CDS             complement(18629..19048)
                     /gene="fabZ"
                     /locus_tag="IVW84_001003"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7917213.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19073..19549)
                     /gene="accB"
                     /locus_tag="IVW84_001004"
     CDS             complement(19073..19549)
                     /gene="accB"
                     /locus_tag="IVW84_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7917214.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19552..20787)
                     /gene="fabF"
                     /locus_tag="IVW84_001005"
     CDS             complement(19552..20787)
                     /gene="fabF"
                     /locus_tag="IVW84_001005"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7917215.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20806..21543)
                     /gene="fabG"
                     /locus_tag="IVW84_001006"
     CDS             complement(20806..21543)
                     /gene="fabG"
                     /locus_tag="IVW84_001006"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7917216.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21555..22472)
                     /gene="fabD"
                     /locus_tag="IVW84_001007"
     CDS             complement(21555..22472)
                     /gene="fabD"
                     /locus_tag="IVW84_001007"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7917217.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22579..22803)
                     /locus_tag="IVW84_001008"
     CDS             complement(22579..22803)
                     /locus_tag="IVW84_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7917218.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22856..23818)
                     /locus_tag="IVW84_001009"
     CDS             complement(22856..23818)
                     /locus_tag="IVW84_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7917219.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23828..24268)
                     /locus_tag="IVW84_001010"
     CDS             complement(23828..24268)
                     /locus_tag="IVW84_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7917220.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6660 a         5429 c         4244 g         8024 t
ORIGIN      
        1 ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca ctttttcgct
       61 aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag caaattgggc
      121 caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag atgtttgtac
      181 gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg caatgatttc
      241 gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc tacctgagtt
      301 tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga gcaagacgtg
      361 gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca gaatatcttc
      421 tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg attccaaaca
      481 ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt ttatcgctac
      541 ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg cttctatatc
      601 actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt
      661 cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac tcaacccgga
      721 agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac cgataatgta
      781 gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa tacgactaat
      841 tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac catatcagac
      901 ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca acagaatatc
      961 ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa tacctgttat
     1021 ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc tattagtttt
     1081 ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc aaatcttcta
     1141 cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc aattccttgt
     1201 ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc ttatagccca
     1261 ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa cgttctttga
     1321 tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag
     1381 gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg acaaaacgtt
     1441 ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt tccccgtctt
     1501 ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt atagttgata
     1561 atgtttcttc tgtaccaaga gctgttttga cttgaacatc tagttttggt ccgtaaaagg
     1621 ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg gctttcagca
     1681 ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta ttgtttggat
     1741 cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat actgccacca
     1801 tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca aatgtatgac
     1861 catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct gatttttcat
     1921 agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa gaatggatcg
     1981 tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca agcatttcgc
     2041 catctcccat atccattggc gggaacatgt cttcttgata atgatcccag tgtccagatg
     2101 ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa cctagactaa
     2161 cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc
     2221 agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt tcttttccta
     2281 gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct ttcaaagctt
     2341 tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt gagtttccac
     2401 gccaataagc acctgccact gataataatt taaatacttg tatacggcca gtggatggaa
     2461 catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta atcacttctt
     2521 cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa atttctaatg
     2581 cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg attttcatca
     2641 tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca ttatctgtat
     2701 cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt ggatagaggc
     2761 gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca agtgcatctt
     2821 catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga gtcaaatcaa
     2881 cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt gaaatgcttt
     2941 cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca tctggaaaag
     3001 taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat catcaagctg
     3061 aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa gttcgtggtt
     3121 ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg accgcccttg
     3181 cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa gtttcagcct
     3241 agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc aatcaattat
     3301 gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact tattttttcc
     3361 attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca tggatgtgtt
     3421 cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc atattttcta
     3481 attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata atgctctctc
     3541 ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt cttgggtact
     3601 gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat agagaccgta
     3661 tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt acttgcaatt
     3721 aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc tattctcatc
     3781 gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt caagatgacc
     3841 gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat accgatatct
     3901 gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac aggtctttga
     3961 tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg ggcatgtaca
     4021 gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc
     4081 atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc tgcttctggg
     4141 cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt tacatagatt
     4201 gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc aatctttact
     4261 tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag ttgactaaca
     4321 ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat atggctagat
     4381 tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc attgactggc
     4441 tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc aaaagcaatc
     4501 gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat cccgttttta
     4561 cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag tggacaaggt
     4621 gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca ccaagaaata
     4681 ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc taaacggaca
     4741 aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg atactggctg
     4801 tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa agaccctgac
     4861 ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa agaagattca
     4921 tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc gccaggcttg
     4981 actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa ttttccttct
     5041 ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc tttacccgct
     5101 cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa
     5161 ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc taatacatct
     5221 acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa gaaggcgaga
     5281 atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa aacaaattca
     5341 cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt
     5401 gttttttctc ctcttttatg tgacttagag aggttagtaa taaatttcca gctgcctctt
     5461 tcatcttaag catagtcctt gactagcact ttttaaaaca ataggcctta attgaaaaca
     5521 actatcacta tcagcgtttc caaaattcat cagttctaca aacaaaaata ttcagcaagg
     5581 attacttttt cgattcatca gttaaaaagt accttttttc gaaaaactat gctatacttt
     5641 taattcgtta tttacggcgg aaggagcaaa catgcgatga atgagaaaca actagaacaa
     5701 acgcacgtgg atgaaacgat cgaactaatc cagatcgaac aaaatatgct ggatacaaaa
     5761 caacaagaat tgactcagga aatgaatcat tcgacaaaag acacagctaa tcacaagatt
     5821 cgagctggtt ccaatgaagc tttttatgaa tctgctgttg aatatcgcca acatgagcag
     5881 gaattgcttt tgaaatacca tactcttgaa gcacagcaaa aacgcctgca aacgttgtct
     5941 gtcatgaaag atagtcctta ttttgcccgt atcgacttta aagaagaccg tgaaacagaa
     6001 acattatatc taggtatcgc ttctttaaga gatctggcag aagaaacaat cgttattgac
     6061 tggcgcgcac caattgccaa tctttattat gaaggtgaga ttggtcccgc attttatgaa
     6121 actgaaacag atagaataga tgtcgatctg cagttgaaac gacaatttaa aatccaagaa
     6181 ggacgtattt tatctatggt agatacgtcg gaagtaatca atgatgactt tttgctagag
     6241 atattggatg aatcatcaag caaccatatg aaaaacatcg tctctacgat acaaaaagca
     6301 caaaatgcaa tcattcggga cacaacaagt aaagtaatgc tgattgaagg aatcgccgga
     6361 agtggaaaga catccgcttt gcttcaacgt attgcttttc tgttgtatca taatcgcaag
     6421 tggctcgagg ctgaaaacgt tcttctattt tcacccaacc atttgttttc tgattacatc
     6481 tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta cacagacatt caaaaattat
     6541 ttggtgcagc tgctgccaga gttttcttta ctagatgaac agcaacaaga aagtggtttt
     6601 ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg gattgacctt agtcgatcag
     6661 attgatcgct atatccaagg catcacaaac tacggtccct tgttccgtga tatgaaaata
     6721 aatggccgta cgatcctatc aaaggaatcg attcgccaat ggtataaaga aacaaatgaa
     6781 caattacctt tacatcatag actttctcta ttacaaacga agctcctgaa aaaattaggc
     6841 ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag atctggcgga agaacagctc
     6901 caagaactat acgcaacaga tccaaatctt gaatatacag aacaaaagga gcgttcatta
     6961 cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc
     7021 atgaattatc aatttgtcaa tataacgaaa caatatctcc actttctaca aactgtgcca
     7081 ctttctcttc taacacgaca taaaatcagc gagaccaggt ggcaggaaca tttgacaact
     7141 gttcgtacac acttgcgtca aagagaattg cgtcaagagg atgctgtact attcttttta
     7201 ctgatgcgcc gaacatatcc agtgacggtt acacacaaag ctcgctatat ctttattgat
     7261 gaaatgcagg atttcgcacc atcacaagtg gcattgctgc aagccttgtt cccaaaagca
     7321 aatttgactt tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga
     7381 tcattggaag accttttcgt ggaccgtgaa gtcactcgtt tccaattaac gacaagctat
     7441 cgctccacaa aagaaatcac tgattttgct aatcatttta ttgtgaaaga acatcaagta
     7501 gaaacaacag caagaaaagg agaccttcct ttgctcatcc aaggaacaac agatgatatc
     7561 aaattgaatt ggttagcaga taccattcga acgactcagg gaaaagcccg gtattggcgt
     7621 acagcaatca tagctaaaac acaagaagaa tgcgaagctc tttatgctca actgccagac
     7681 gagttgaaac aacaagtcca gctgatttta aatgagagtg actttatgaa gcgttccatt
     7741 attttacttc cagccttttt agcaaaaggt ctggagtttg accgagtttt cttgtgggat
     7801 gtagatgcag aacatttttc ttctcagcaa gacaaattga tcctttacac catgtgtaca
     7861 cgtgcgatgc atgaattgac cctttttacc tccaataatg aaagtccttt gttaaacaat
     7921 atggatattt catcttatga gaaaaaagta ctgtaagtaa aaagacaact gctatcatta
     7981 atgttagcag ttgtcttttt atttacaata ataattgcag aaaaagtaat tcaatcattt
     8041 aaggtcattt attctatata taaatccaaa tctgtttcag gatattttaa tttgacaagc
     8101 gaaataatca ttttcagttc ttccatgttt tctggatatt ctgggtcaaa taccttgcta
     8161 tgacggccaa tcccgccatt gtcgatcaat cggtaaattc gattccattc aatgaattgg
     8221 tcaattgatt ggaaattgct gattggatag ccgccacaat ccaaaatctc tgatacacga
     8281 aatgctaggt atgcactatc gatcacttta ttttttttcg tgtctatctt ctcatttctt
     8341 tcgataagaa ttttttcagg acgatgtttc aagctccact ctaatagtag aaagaaagcc
     8401 tccatccgat actcttcttc aaataaccga aaggctgaat cagaacgacg aaaaagcagc
     8461 ggaatcagat aattttgtct ataaatcagc attggtgcgc ggtccaccat acactctaaa
     8521 taacggttca gataccctgt accgacatag tttcctaatc gatagccttc aacgccgaat
     8581 cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa ctcttctact
     8641 ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag tgatttttgc ttccatattc
     8701 ttccccgtcc atcttccaca tacttcatat catttcacat gatacttatg ctctcatcat
     8761 accacaggtt gaattagaaa agaaaataaa tcacacaaaa aagcaagaca atctgttagt
     8821 gagattgtct tgcttttcga aaaagactga atacttttcg ttaaaactgg ttgcttagtc
     8881 ttctaatgcc acattatgga acacatgctg tacatcttct agatcttcca gtgcatcaat
     8941 cattttttca aatgtttctt gatcttctgg agatagtgtt acctcgtttt gagggatcat
     9001 ttggatctca gcgattgaaa aatcagtgat tcctttttct tttaatgctt cttgtatact
     9061 atggaaatcc gctacttcac cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg
     9121 aacatcgatg tctttttcca ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa
     9181 agcaaagatt gctgtattgt caaacatgta agaaaccgct ccggaaacac ccatgttccc
     9241 gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa
     9301 tgtatcaaca atcaccattg agccgcttgg tccaaatcct tcatagcgca attcagagta
     9361 tgtttcatct cctgcacctt ttgctttttc gattgctcgg tcaataatat gttttggtac
     9421 attataagtt ttagcgcgat caatgacgaa acgtagtttt tggtttgcat gcggatcagg
     9481 atcacctgat tttgctgccg cgtagatttc aatcccaaat tttgcataaa tccggctatt
     9541 attagcatct ttagcagttt tctttgctac gatatttgcc catttacgtc ccatattttc
     9601 acttcgcttt ctgatttgat agtttcataa cgcaaaaacg tcctgttcca ttatacttgt
     9661 gaatcacgat tctgtgaagc tttttttctt tcttccttca ttcttctcat ttttagtcaa
     9721 ttatctatac tcggattggg atttgcccga aattctgttc cggaatattg cctcccaatt
     9781 gatagatttg atcacttcta actaacagtt ccgcttcttg tgcctctttt tttccgattc
     9841 gggaataaat agaaatctct tttttttctt ttaagaatct tcttccagca tcggtaaatc
     9901 caagtagtcg aagataattt ttattttgtt ggacaggaat ctcttgcttt ttgatattca
     9961 gcaagacata acaagataat cgttggatcc gtgtccatgt atatcttttt gtttttacct
    10021 ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg cgcgattcta
    10081 ttccttcggt catctgatag atatctgtca gttccagcaa agtagaagat aacaaccgat
    10141 aacgcaaata cggccaataa gattcccaag tctgcagcga acaagttgaa agatcttctt
    10201 ctgtctgagg cggcacaagt ccagctattc tttttccttc ttctaacgca tggcgaatgg
    10261 ctgttgcact ggctatcctc tgatctgtga tgattttttc atgatagcct gcacttttac
    10321 gtcggatcgg atgtaattcc atagggtgga catacttggc attttccttt gcataactta
    10381 atcccaagat atgatttggc gaagaaaaat caagcgtaat atcgggatac agactgcgaa
    10441 aaacctcagt cattttttgc ggataactat acgtttgatc agacagttct tgaaatgcct
    10501 cttctatcct atcttgattc tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag
    10561 tctcttcttc tgttccaaaa cacaaactgt cacagtccag tgtttgcaag atttttactg
    10621 cacctttagc aaaatagtca gctgactgaa ccgcccattc gacgggaagc tccactacta
    10681 aatccacacc attttgtaaa gcttcattcg cgcgatgcca tttatcgatg atagccggtt
    10741 cgcctctttg caaaaaattc ccgctcatta cagcaatgac gacctctgct cctgttattt
    10801 ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa aggattgtac tctacgataa
    10861 tcccgcagct tttcatgcgc atcctccttc atagttatcg ataaaaaaga aatagagaca
    10921 agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat ataaactatt ctcgcaaaca
    10981 caatacttct gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt tcggatatat
    11041 aatttatgtt gaagatttat tcttttcttg ttaactcgag caggcaaaac cgctagtctt
    11101 tgtatgctac aaagaaccag cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag
    11161 aaaatgcttc gactttcgaa aagccgctgt tttccagcat tcttaaataa ctttccaatg
    11221 gataggtacg ctcttgatgt agttcatctt cacgaataaa tcgaccagac ccttcttgtt
    11281 cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct ggataactat
    11341 cccacaaaaa agcaaaatta tccccttgat aatgatagct atattctgga aaaacctcat
    11401 ctacttgata ggtggagtgt acgtcaaata aaaatcggcc attttcttct aacgcttgat
    11461 agacttcatc aaacacttgc tgtacctctt gtctattttt catatagcac agcgaatcag
    11521 aaaaacaggt aattgcctga tattgtccga tttctgataa atcgagcata ttgccttcca
    11581 caaattggat cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc aacatctctt
    11641 ctgaaaggtc taaccctgtc actttatatc catcttttgc aaaggcaacc gctaatgctc
    11701 cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa
    11761 aagctaacca ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg
    11821 tttcataagc catatgttaa tccaaccatt cactaagatc gactagcgga gcatctgacc
    11881 atagtttttc cagattatag aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat
    11941 ctccaagatc gatcaatacc cattttccgc cttcttttcc ttcaatacgt tttacttcat
    12001 atttgttttc ttcttcttta tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc
    12061 tatttgctga acagatcatg aaataatctg ccaacaagga aacatttcga acgtctaaag
    12121 cgataatgtc ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt aattgttctt
    12181 tactatctat gatgatttcc tcctaatatg agcagcttat ttttttgcta cccagtaatt
    12241 atatgtttcg attgtttttg gataaatctg ttgttcttgc gcaagcaaat gggccaatgt
    12301 atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc
    12361 ttgtacccca ggaaaatctc gacctggttc aatatagtct gctacataaa tgattttgtc
    12421 aaggagactc atcttagcag ccccagtcgt atgcacccgg attgcatgaa gaatctctgc
    12481 atcggtgatt cctaattccc gctctacaaa cgaagcgcca acaagaccat gccagattgc
    12541 attattataa ttcaataact ccggatcaaa accgtctctt acgattacca ttttgaactc
    12601 ctcatctggt cgttcttttg cataatcatg tgtcaatgca gcaatactag ctttttcagg
    12661 agaagcaccg tattttttgg caagagccac ggctgtttct tctactccaa gcacatgctt
    12721 gaagcgttgt tcgctcattc tcatctgtat tttttgcatc aggttttctc tagaataagc
    12781 agtatattca ttactatagt ccatattcat acaacccttc attttgaata taatcaatta
    12841 ctttatctgg aacaaggtag cggatagaac agccttcttt gatcttttga cgtattttcg
    12901 tggaactgat gtcgatctca ggtacatcta cccaaattac aggatatgga gtatccgttg
    12961 tgtatcctgg tcgacgtata cccacaaagt tgaccataga agtcagctca tcgattttat
    13021 accattttgg caaatattct accatatccc caccgataat aaagtaataa tctgtatccg
    13081 gattgttttg cgtcaattcc ttcatagtat cataggtgta acttttccca ccacgagcta
    13141 attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc aacatgttga
    13201 gtcgatgttt tgcatcaatc gtctgtttct catcaacatg aggtggctga taagaaggca
    13261 tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg ttcagccatc actaaatgtg
    13321 caaggtgaac gggattgaat gttccgccca agatccctac ttgctttttc ttctctaaga
    13381 attgaggcat ttcttcagga aataattgtg aacgaacaaa tgtcttagct tgcgtattca
    13441 tcatggtcac tccttatatt gcttttactt cttttgatag cctttgatac ttttcttttg
    13501 tggaaggttt gtataataca ataacacgtc caatgatttg gacgatttcg caatctattt
    13561 ctttttctaa tacttgagct gcttcttctg caatctcatc tgtattttga agtaacgata
    13621 ctttgatcag ttctcttttt tctaaagctt cttcaatttg aacgatcatt gctgaattga
    13681 tcccaccttt tccgatttga aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac
    13741 gcttttgttt tcctcttaaa ctcataattt tccttcttcc ttaaatcaat gctttgcgtt
    13801 tcaatacatc tacacccttt ggcgcccagc ccgcaattac tactggcgct gttacagtga
    13861 tccagcctag accggcaaaa acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa
    13921 caagctccgg aaatgcctcg acttcatcag gacgaggagg ctgtaataat ccacctacat
    13981 gtttttgata aaattcgtct gctttttcca gtttcgttcg atgaatattt acatcatttg
    14041 acacataagc aataaatgaa gcacgattgc cgctgataaa atcaaatctt gcaagtccgc
    14101 ccaagaaaag tgtttgccct tcattcagct gataaacttt aggtttgatt tcttttaacg
    14161 gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc catttgatgg cgatggataa
    14221 ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg aatctcgatt ttatccaagg
    14281 tcgttcctgg aaactgcgaa gttgtgatta catcttgtac tcccgctgta tttttgatga
    14341 tttgattgat aagcgtcgat tttccgacat ttgttactcc gacgatatag acatcccgtc
    14401 catcgcggta ttcttcgatt ttttctaata gttccgtcat ctcatgagat ttcttagcac
    14461 tagttaataa tacatctact ggacgaattc ctgcttcgta agcacgttcc ttcatccatt
    14521 gtgttaattt agtttttttc aatgatttag gtaatatatc cactttgttc ccaactaaaa
    14581 gtactggatt gtcaccgaca aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa
    14641 aaatatccac cacattgacg atcaatgcat ctttttgccc taattcattc agtaagcgca
    14701 aaaagtcgtc atccgtcaat gagacatctt gggtctcatt ataatggcgt aatctgaaac
    14761 aacgctgaca atagacttcc ccggaagcta aacctttttc caaagccgtt tgcggcgtgt
    14821 atcctaattc accgggtttg tttgtttgta taacggcacc acagccgata cattgtaatt
    14881 cttctgtcat tcgattccac ctcgccagcc catatcaggg tgttttgcta atagataggc
    14941 cataattctt ttttcccaaa aacgattaaa ttgtgtcttc caactatctg ttgcgataat
    15001 gggttttaca aggatgctac gtatccctgc acgattggct ccacgtatat cagtcataat
    15061 ctgatcgcct accattacta tctcatcatc tgctaaatcc aattgtttct ttgctatgtt
    15121 gatccctctt gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa atttttcaat
    15181 cgctcgcgcg acacgttttg aattattgtt tgaaacaaca acaacaggga ttcctgcatt
    15241 tttcatttct aaaatccaag taagcaactc ttctgttcca tctggattat tccatgcaat
    15301 caacgtattg tctaaatcgg tcaatactgc tttgatgcct agattcttta attgggcagg
    15361 tgtaatctga tagatggctt ctaccatcca tgttggtttg taatttgaaa acatgctgtc
    15421 tcctcatctg caaaaaatga aggagcacat aaagtactcc tcaaccccgt attatactgc
    15481 atatcagcaa aaaaaactac ctctatactc gttcgtcttt cagaaaaagc atttctttac
    15541 aaaatgacca aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa caactaatat
    15601 tggcggaacc tcttgtatcg tttttccagt aatttatgct gatctaatcg aacaagttcc
    15661 gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc
    15721 tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc
    15781 gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc
    15841 gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca
    15901 tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg
    15961 atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg
    16021 cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaaggt aatcaccggt
    16081 ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg
    16141 ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc
    16201 actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca
    16261 aataaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag
    16321 cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg
    16381 atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct gtttcaattc
    16441 catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa
    16501 atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat
    16561 caagctttgg ggttctgcta gaataatatc gccttccatt gcaaaactgg cagttactcc
    16621 tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt
    16681 gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc
    16741 gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa
    16801 tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat taggatccat
    16861 cactccaata atgaagggaa tcccctgaat ggttgcttta ccagtgagga ctgcttcatg
    16921 aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg
    16981 atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc
    17041 tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc
    17101 accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc
    17161 aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt
    17221 ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg
    17281 taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg caggatgact
    17341 aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa
    17401 ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc
    17461 aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctactcg
    17521 cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa
    17581 atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac
    17641 ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa
    17701 atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc
    17761 catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata
    17821 attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga
    17881 gatcactaca gaaggagatt cttccagtac tttttgattg ttgcgttgta aagaacaatc
    17941 tcgctcgcct aaatgaatga catgaccaaa tcgatcacct aagatctgaa cttctatatg
    18001 gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc
    18061 ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat
    18121 gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc
    18181 tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac
    18241 atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc
    18301 acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt
    18361 ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct
    18421 cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc
    18481 ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt
    18541 ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc
    18601 tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg
    18661 ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt
    18721 tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg
    18781 ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg
    18841 caactgctcc tgcttgagcc attgcttcaa caatcaatac ccctggcata acaggttctt
    18901 ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc
    18961 gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa
    19021 tgatttcttt tatttcttgt atgttcattg ttttccctct ttcctgcttt gcttattttt
    19081 gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt
    19141 ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa
    19201 tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg
    19261 gcgctggttg cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata
    19321 ctgccgattc tttttctttc attatttctg gagcagttgt tgcttgttca ggtatgacat
    19381 tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg
    19441 ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg
    19501 aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc
    19561 ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc
    19621 ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc
    19681 ttgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc
    19741 caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct
    19801 gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg
    19861 gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac
    19921 accagcttct tccatcgcaa gcttcattgc tttagatgca ccagaaccat ctggagtagg
    19981 agctgtcata tggtaagcgt cacagtttgc accgtatcca acgatttctc catagatttt
    20041 ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacgcctg ctccttcacc
    20101 catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga
    20161 ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc
    20221 tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc
    20281 aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc
    20341 tacacgcaaa gcaatatttc cagctgccat gttggcaatc gccattggta cgaacattgg
    20401 agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag
    20461 accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc
    20521 taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc
    20581 cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc
    20641 cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt
    20701 cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc
    20761 atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac
    20821 caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac
    20881 tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc
    20941 ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc
    21001 aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc
    21061 tactacgcca gctttactag ctgcataatt tgcttgacca atatttccaa tcaacccaga
    21121 tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg
    21181 ctgcgtcata ttgaaggttc caatcaaatt gatatccaga cattttttga aatcttctgc
    21241 atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat
    21301 cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat
    21361 gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt
    21421 tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc
    21481 catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc
    21541 cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca
    21601 cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg
    21661 ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa
    21721 cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt
    21781 tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg
    21841 tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca
    21901 tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact
    21961 gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg
    22021 atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc
    22081 actgcaacca ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt
    22141 gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat
    22201 tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga
    22261 taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt
    22321 tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca
    22381 aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca
    22441 ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat
    22501 gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc
    22561 ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac
    22621 cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat
    22681 gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt
    22741 gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac
    22801 cataataata tattcctccg atattgtttg ctttttattt ttataattta acagtctaca
    22861 actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct
    22921 gctgttttcc taaggtgatc tttccactag aaactgcttc atccaataag atagggatgc
    22981 ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct tgcggcatct
    23041 ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat
    23101 caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat
    23161 caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga
    23221 acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg
    23281 ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc
    23341 catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt
    23401 caccaccaat caccagtccc ttagttgagc cgcagcggat cattttttca gccatgctta
    23461 acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc
    23521 ccaatttccc ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc gacattgttg
    23581 ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg
    23641 ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac
    23701 ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag
    23761 taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta
    23821 aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg
    23881 catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat
    23941 gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc
    24001 ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag
    24061 tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct
    24121 tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag
    24181 attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat
    24241 catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca
    24301 aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt aaaagat
//
LOCUS       DACCWV010000028        23730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCWV010000028 DACCWV010000000
VERSION     DACCWV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.028"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            155..586
                     /gene="mraZ"
                     /locus_tag="IVW84_001011"
     CDS             155..586
                     /gene="mraZ"
                     /locus_tag="IVW84_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7917221.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            607..1566
                     /gene="rsmH"
                     /locus_tag="IVW84_001012"
     CDS             607..1566
                     /gene="rsmH"
                     /locus_tag="IVW84_001012"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7917222.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1571..1972
                     /gene="ftsL"
                     /locus_tag="IVW84_001013"
     CDS             1571..1972
                     /gene="ftsL"
                     /locus_tag="IVW84_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7917223.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1973..4165
                     /locus_tag="IVW84_001014"
     CDS             1973..4165
                     /locus_tag="IVW84_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7917224.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4191..5153
                     /locus_tag="IVW84_001015"
     CDS             4191..5153
                     /locus_tag="IVW84_001015"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7917225.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5167..6528
                     /locus_tag="IVW84_001016"
     CDS             5167..6528
                     /locus_tag="IVW84_001016"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7917226.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6554..7642
                     /gene="murG"
                     /locus_tag="IVW84_001017"
     CDS             6554..7642
                     /gene="murG"
                     /locus_tag="IVW84_001017"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7917227.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7657..8877
                     /locus_tag="IVW84_001018"
     CDS             7657..8877
                     /locus_tag="IVW84_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7917228.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9033..10358
                     /gene="ftsA"
                     /locus_tag="IVW84_001019"
     CDS             9033..10358
                     /gene="ftsA"
                     /locus_tag="IVW84_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7917229.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10381..11622
                     /gene="ftsZ"
                     /locus_tag="IVW84_001020"
     CDS             10381..11622
                     /gene="ftsZ"
                     /locus_tag="IVW84_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7917230.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11639..12316
                     /locus_tag="IVW84_001021"
     CDS             11639..12316
                     /locus_tag="IVW84_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7917231.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12330..12929
                     /locus_tag="IVW84_001022"
     CDS             12330..12929
                     /locus_tag="IVW84_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7917232.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12944..13231
                     /locus_tag="IVW84_001023"
     CDS             12944..13231
                     /locus_tag="IVW84_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7917233.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13280..14062
                     /locus_tag="IVW84_001024"
     CDS             13280..14062
                     /locus_tag="IVW84_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7917234.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14083..14790
                     /locus_tag="IVW84_001025"
     CDS             14083..14790
                     /locus_tag="IVW84_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7917235.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15052..17850
                     /gene="ileS"
                     /locus_tag="IVW84_001026"
     CDS             15052..17850
                     /gene="ileS"
                     /locus_tag="IVW84_001026"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7917236.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17899..19425)
                     /locus_tag="IVW84_001027"
     CDS             complement(17899..19425)
                     /locus_tag="IVW84_001027"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7917237.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19440..20087)
                     /locus_tag="IVW84_001028"
     CDS             complement(19440..20087)
                     /locus_tag="IVW84_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7917238.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20271..20600)
                     /locus_tag="IVW84_001029"
     CDS             complement(20271..20600)
                     /locus_tag="IVW84_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7917239.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20777..21505
                     /gene="sfsA"
                     /locus_tag="IVW84_001030"
     CDS             20777..21505
                     /gene="sfsA"
                     /locus_tag="IVW84_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7917240.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21521..22534
                     /locus_tag="IVW84_001031"
     CDS             21521..22534
                     /locus_tag="IVW84_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7917241.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22534..>23730
                     /locus_tag="IVW84_001032"
     CDS             22534..>23730
                     /locus_tag="IVW84_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7917242.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNP"
BASE COUNT         8039 a         4091 c         5080 g         6520 t
ORIGIN      
        1 tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata
       61 aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac
      121 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
      181 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
      241 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
      301 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
      361 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
      421 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
      481 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
      541 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
      601 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
      661 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
      721 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
      781 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
      841 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
      901 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
      961 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
     1021 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
     1081 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
     1141 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
     1201 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
     1261 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat
     1321 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
     1381 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
     1441 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
     1501 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
     1561 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
     1621 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
     1681 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
     1741 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
     1801 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
     1861 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
     1921 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
     1981 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
     2041 aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg
     2101 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
     2161 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
     2221 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
     2281 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
     2341 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
     2401 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
     2461 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
     2521 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
     2581 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
     2641 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
     2701 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
     2761 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
     2821 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
     2881 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
     2941 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
     3001 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
     3061 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
     3121 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
     3181 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
     3241 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
     3301 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
     3361 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
     3421 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
     3481 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta
     3541 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc
     3601 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
     3661 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
     3721 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
     3781 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
     3841 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
     3901 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
     3961 ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg
     4021 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
     4081 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
     4141 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
     4201 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
     4261 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
     4321 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
     4381 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
     4441 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
     4501 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
     4561 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
     4621 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
     4681 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
     4741 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
     4801 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
     4861 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
     4921 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
     4981 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
     5041 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
     5101 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
     5161 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
     5221 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
     5281 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
     5341 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
     5401 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
     5461 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
     5521 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
     5581 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
     5641 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
     5701 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
     5761 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
     5821 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
     5881 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
     5941 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
     6001 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
     6061 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
     6121 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
     6181 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
     6241 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
     6301 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
     6361 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
     6421 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
     6481 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
     6541 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
     6601 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
     6661 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
     6721 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
     6781 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
     6841 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
     6901 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
     6961 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
     7021 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
     7081 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
     7141 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
     7201 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
     7261 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
     7321 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
     7381 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
     7441 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
     7501 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
     7561 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
     7621 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
     7681 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
     7741 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
     7801 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
     7861 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
     7921 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
     7981 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
     8041 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
     8101 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
     8161 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
     8221 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
     8281 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
     8341 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
     8401 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
     8461 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
     8521 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
     8581 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
     8641 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
     8701 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
     8761 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
     8821 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
     8881 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
     8941 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
     9001 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
     9061 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
     9121 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
     9181 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
     9241 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
     9301 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
     9361 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
     9421 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
     9481 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
     9541 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
     9601 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
     9661 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
     9721 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
     9781 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
     9841 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
     9901 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
     9961 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
    10021 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg
    10081 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    10141 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    10201 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    10261 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    10321 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    10381 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    10441 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    10501 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    10561 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    10621 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    10681 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    10741 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    10801 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    10861 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    10921 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    10981 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    11041 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    11101 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    11161 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    11221 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    11281 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    11341 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    11401 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    11461 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    11521 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    11581 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    11641 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    11701 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    11761 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    11821 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    11881 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    11941 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    12001 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    12061 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    12121 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    12181 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    12241 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    12301 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    12361 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    12421 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat
    12481 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    12541 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    12601 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    12661 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    12721 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    12781 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    12841 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    12901 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    12961 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    13021 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    13081 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    13141 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    13201 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    13261 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    13321 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    13381 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    13441 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    13501 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    13561 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    13621 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    13681 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    13741 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    13801 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    13861 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    13921 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    13981 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    14041 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    14101 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    14161 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    14221 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    14281 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    14341 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    14401 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    14461 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    14521 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    14581 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    14641 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    14701 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    14761 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    14821 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    14881 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    14941 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    15001 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    15061 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    15121 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    15181 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    15241 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    15301 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    15361 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    15421 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    15481 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    15541 gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt
    15601 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    15661 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    15721 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    15781 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    15841 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    15901 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    15961 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    16021 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    16081 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    16141 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    16201 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    16261 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    16321 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    16381 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    16441 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    16501 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    16561 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    16621 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    16681 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    16741 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    16801 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    16861 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    16921 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    16981 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    17041 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    17101 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    17161 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    17221 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    17281 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    17341 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    17401 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    17461 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    17521 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    17581 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    17641 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    17701 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    17761 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    17821 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    17881 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    17941 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    18001 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    18061 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    18121 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    18181 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    18241 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    18301 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    18361 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    18421 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    18481 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    18541 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    18601 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    18661 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    18721 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    18781 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    18841 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    18901 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    18961 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    19021 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    19081 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    19141 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    19201 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    19261 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    19321 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    19381 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    19441 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    19501 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    19561 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    19621 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    19681 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    19741 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    19801 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    19861 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    19921 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    19981 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    20041 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    20101 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    20161 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    20221 aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca
    20281 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
    20341 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
    20401 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
    20461 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
    20521 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
    20581 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
    20641 gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta
    20701 agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga
    20761 agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc
    20821 gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa
    20881 aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc
    20941 tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg
    21001 gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac
    21061 gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct
    21121 tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt
    21181 caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag
    21241 aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt
    21301 cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc
    21361 agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa
    21421 ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat
    21481 cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat
    21541 cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga
    21601 attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga
    21661 aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac
    21721 catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc
    21781 aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat
    21841 catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga
    21901 atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa
    21961 tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa
    22021 tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata
    22081 tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc
    22141 tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat
    22201 ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac
    22261 gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa
    22321 agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga
    22381 ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg
    22441 gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat
    22501 caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc
    22561 gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga
    22621 gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca
    22681 ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt
    22741 gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt
    22801 gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa
    22861 cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata
    22921 ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat
    22981 gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa
    23041 gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc
    23101 attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct
    23161 cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg
    23221 agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa
    23281 ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct
    23341 gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga
    23401 gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta
    23461 gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt
    23521 cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta
    23581 aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag
    23641 ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg
    23701 aaaacttttt ctggaaagga gcagaaccca
//
LOCUS       DACCWV010000029        23640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCWV010000029 DACCWV010000000
VERSION     DACCWV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.029"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(243..1025)
                     /locus_tag="IVW84_001033"
     CDS             complement(243..1025)
                     /locus_tag="IVW84_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917243.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1090..1926)
                     /locus_tag="IVW84_001034"
     CDS             complement(1090..1926)
                     /locus_tag="IVW84_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7917244.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1944..3353)
                     /locus_tag="IVW84_001035"
     CDS             complement(1944..3353)
                     /locus_tag="IVW84_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7917245.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3367..5190)
                     /locus_tag="IVW84_001036"
     CDS             complement(3367..5190)
                     /locus_tag="IVW84_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7917246.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6033..6359)
                     /locus_tag="IVW84_001037"
     CDS             complement(6033..6359)
                     /locus_tag="IVW84_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917247.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6405..6767)
                     /locus_tag="IVW84_001038"
     CDS             complement(6405..6767)
                     /locus_tag="IVW84_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAP7917248.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6804..7018)
                     /locus_tag="IVW84_001039"
                     /pseudo
     CDS             complement(6804..7018)
                     /locus_tag="IVW84_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7146..7481
                     /locus_tag="IVW84_001040"
     CDS             7146..7481
                     /locus_tag="IVW84_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7917249.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7557..9554)
                     /locus_tag="IVW84_001041"
     CDS             complement(7557..9554)
                     /locus_tag="IVW84_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917250.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9652..9927)
                     /locus_tag="IVW84_001042"
     CDS             complement(9652..9927)
                     /locus_tag="IVW84_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917251.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10159..10893
                     /locus_tag="IVW84_001043"
     CDS             10159..10893
                     /locus_tag="IVW84_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7917252.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10932..12074
                     /locus_tag="IVW84_001044"
     CDS             10932..12074
                     /locus_tag="IVW84_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7917253.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12169..13560)
                     /gene="sufB"
                     /locus_tag="IVW84_001045"
     CDS             complement(12169..13560)
                     /gene="sufB"
                     /locus_tag="IVW84_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7917254.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13565..14035)
                     /locus_tag="IVW84_001046"
     CDS             complement(13565..14035)
                     /locus_tag="IVW84_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7917255.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14022..15257)
                     /locus_tag="IVW84_001047"
     CDS             complement(14022..15257)
                     /locus_tag="IVW84_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7917256.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15254..16540)
                     /gene="sufD"
                     /locus_tag="IVW84_001048"
     CDS             complement(15254..16540)
                     /gene="sufD"
                     /locus_tag="IVW84_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7917257.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16553..17326)
                     /gene="sufC"
                     /locus_tag="IVW84_001049"
     CDS             complement(16553..17326)
                     /gene="sufC"
                     /locus_tag="IVW84_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7917258.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17523..18371)
                     /locus_tag="IVW84_001050"
     CDS             complement(17523..18371)
                     /locus_tag="IVW84_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7917259.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18395..19081)
                     /locus_tag="IVW84_001051"
     CDS             complement(18395..19081)
                     /locus_tag="IVW84_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917260.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19074..20108)
                     /locus_tag="IVW84_001052"
     CDS             complement(19074..20108)
                     /locus_tag="IVW84_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917261.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20503..20841)
                     /locus_tag="IVW84_001053"
     CDS             complement(20503..20841)
                     /locus_tag="IVW84_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7917262.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20843..21199)
                     /locus_tag="IVW84_001054"
     CDS             complement(20843..21199)
                     /locus_tag="IVW84_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7917263.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21205..22392)
                     /locus_tag="IVW84_001055"
     CDS             complement(21205..22392)
                     /locus_tag="IVW84_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7917264.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22583..23428)
                     /locus_tag="IVW84_001056"
     CDS             complement(22583..23428)
                     /locus_tag="IVW84_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7917265.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
BASE COUNT         6938 a         4998 c         4020 g         7684 t
ORIGIN      
        1 taaaatatac aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa
       61 acacttatat atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata
      121 tagtcgggct ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga
      181 tgtataagta cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat
      241 cattattcaa taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat
      301 accacagaat aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact
      361 tgctctgtca ctgcattact gatacctgtt agattagcta ataaatctga taaagattga
      421 gcatctgtag aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc
      481 ttccaagtag gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc
      541 gtcttcccag tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc
      601 gtctgctccg taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa
      661 cttatatatt gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat
      721 tcactataac cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa
      781 gaaaaataga catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct
      841 acttgagacc ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc
      901 ttattattaa tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa
      961 cactttacca cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt
     1021 tccaaggaat caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat
     1081 tttcttcatt cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt
     1141 tgctgaggca cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc
     1201 tttcggaaaa cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc
     1261 cttcttaatc attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat
     1321 cgaatcttta tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt
     1381 tagtgctctt accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac
     1441 aatgtgaaac gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt
     1501 gatcaattgg atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc
     1561 aatcagcagg cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa
     1621 gtgatcaacc aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc
     1681 ataatcgata attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt
     1741 aatgtaatcc ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt
     1801 gtcagccgga accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat
     1861 tacttctttg ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac
     1921 tctcattaga ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac
     1981 caaaagaagc tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca
     2041 acatatataa aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct
     2101 gaccaaggta aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga
     2161 atatgtttgg ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact
     2221 atgtcgggtg ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac
     2281 aatttcctca atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta
     2341 tctacataag gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga
     2401 tcccccgctc tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt
     2461 gaataataat taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct
     2521 gcctcaattt taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat
     2581 tttccccgaa ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct
     2641 gcaattgcat cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga
     2701 ccgatttttc ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat
     2761 aaaacatggt gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc
     2821 aaaccaatcg ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag
     2881 ttcaccttgt caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata
     2941 gttgctcgat ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac
     3001 atggtgacaa tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa
     3061 gaaattccct tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg
     3121 gaaaagcgaa aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga
     3181 tggtaaaaat ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca
     3241 ataatagacg gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg
     3301 gaggcacccc ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc
     3361 ctctccttaa ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact
     3421 aattcctgcg taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc
     3481 gtttctaatg gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc
     3541 tgcttttaca aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg
     3601 gatcaataat tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat
     3661 agtaatgact tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac
     3721 gccctttccc aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac
     3781 ggttcctttt accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc
     3841 atcagaagta tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat
     3901 tacacacgta ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa
     3961 ggctgctaag ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa
     4021 accagcaatt gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac
     4081 tccatagagt gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag
     4141 ctgcttcaac tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc
     4201 aatattagaa gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg
     4261 catagtagcg attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa
     4321 accacccatc aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat
     4381 tgctataaga tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc
     4441 cataattaaa aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg
     4501 atccgcaaag ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga
     4561 tcccgcataa tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga
     4621 aaaaagctgt gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg
     4681 gttgacatca aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc
     4741 tgcatcagca atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt
     4801 caaagtcaag acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat
     4861 cggtgtaaag gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc
     4921 tgaagagggg ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt
     4981 ttggacttca ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac
     5041 accaagtgtt ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg
     5101 cagtctggtg gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa
     5161 tacatttctt cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga
     5221 ccaagtcaaa ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca
     5281 taatcagtaa catgccaata acgttatttt gctgtaatcc aataatagca cattatgata
     5341 gcgcaatcaa cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa
     5401 cagttagatc acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc
     5461 taatattaac tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg
     5521 tgaaagcatc aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa
     5581 aaattgatga atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac
     5641 aatagtgata cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc
     5701 gagatataaa ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa
     5761 tgtttaaact gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc
     5821 aataaaaata ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta
     5881 taacagtgaa taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat
     5941 tagtatttaa tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt
     6001 cctgtttgga cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat
     6061 ggtatctcca catatatacg gtctttcttt ggatagtact caatatttat gttgatagca
     6121 cttccattgt ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc
     6181 gtcacatcat cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat
     6241 ggtttcgatg acttctctaa tccagatgta cctgtcattt ttgaaaaaat gatacctata
     6301 atagccaaga caacgataac tataatgata aacttcttgt tcatatttac tttattcaac
     6361 tttttatctc ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc
     6421 cctaaaagta caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt
     6481 tgttcatctt tatctcgata taagtagccc ataataacag caacagcacc aaatacaaca
     6541 ggaataaatg ctaacgctac taccatagaa atccaaccaa taatcaaata cagcttccct
     6601 gtctcttgtc catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc
     6661 tttactgcgt ctgctccagt tgtctcatct gtttcttgta gtgctccgca aaactggcaa
     6721 tacttacttt catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct
     6781 aaaaacattc aattgtttat tagttaactg tttactttat taaatacatc agaaaacagc
     6841 tcattcattg gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc
     6901 gttctccctg tttgccacga acttacacat tgctgtgtaa cattcatttt tttgctaact
     6961 ccgtttgaga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     7021 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa
     7081 aaaaacttca aataccatta caaatacaac aacttgttgt actatactga gatgggggtg
     7141 tattaatgaa acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt
     7201 ggacacaaaa agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg
     7261 aaaattctgt aacagagcct aacctcgata gcttaaccgc tctttctaga gccctaggaa
     7321 cccctgtaag ccattttcta gacaatgttg tcgtagatta tgaggaagag tttttggctc
     7381 tctatcgttc tcttagcact gaagatgcag ttcgaacaat tgactatatg aagctattaa
     7441 aaaggcaaga aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga
     7501 actttaacgg tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag
     7561 tttaatacaa ttgggtcttt tctcttcctt actctccgtc catcatctct tgttacactt
     7621 gtaaattgat aaagaggaac taattcaact cctgataata tcaattcttt tattacttca
     7681 tctatccctg ctccttttgc taggtagact aaaaaactac tacttgcttt cacattgtat
     7741 agtctcttaa tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct
     7801 ctgttttctt ttaaagcatt ttccacgctg ttattcccaa cattacgtag acgttcacaa
     7861 acagtgttag taagttcttt ctctgtcatt agatatgttc cttgccacga taagccctgt
     7921 aaaacttcat gtgcttcatc taaaatcaat tgtttttcct tgctaagttc tgatcgggaa
     7981 ttttgaggaa atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa
     8041 gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg gaaggatatc ttcattcaat
     8101 ttttgaatca cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatga
     8161 ttaaagtttc ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa
     8221 ggaacatctt catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac
     8281 ttattacatt tatctatgtc ataagtttta ccatagtaaa aactcatttt tttagctaat
     8341 tgttcccccg acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca
     8401 gaaagcaaga catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa
     8461 agtttgtctt tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt
     8521 cgaaaatgtg taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga
     8581 ctgtctttct ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta
     8641 ctggaatgac aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga
     8701 ttatgatcgt tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata
     8761 tcaatggtta acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg
     8821 cagtctttaa accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa
     8881 tcggtttcat tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat
     8941 gtcatggtaa aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt
     9001 atcactgcat ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca
     9061 tcacacccgg ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca
     9121 gtaattgctt cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga
     9181 atactttttt gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct
     9241 atgcttaagt atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta
     9301 acagtgttat cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg
     9361 cctttattct ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac
     9421 tcatctcttg taacttctct atagttatct aaacacctat ctcttacatc agttttttct
     9481 ttttttgtaa ctggcttctc taataatttt tctttatcta cataaattgg aatattaatc
     9541 attgatgttt ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag
     9601 ataataaaaa caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt
     9661 ataccgttcc atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt
     9721 tataacaggt aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag
     9781 atattcactt gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc
     9841 tagcataggc tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac
     9901 aatgctatca atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa
     9961 ttttctaaga attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga
    10021 ttaatcaaac aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa
    10081 gaggtgtcaa tatctttgta aaatttattt acaacacccc tttttcgagg tatactaatt
    10141 ttaggaggtg tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag
    10201 atactcggaa caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt
    10261 ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga atgggagaag
    10321 ggaacctatt taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt
    10381 atagaggagt tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga
    10441 gatagcattg tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt
    10501 caacagttcc caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta
    10561 gaaccagaga tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa
    10621 attagtcttt tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa
    10681 tttgacaata acattctttt cctcactagc agtatagaag aactactaga aagaatggta
    10741 gatgaatggc tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa
    10801 attactgata atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact
    10861 aagaaaaaaa tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag
    10921 gagatgattt catggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga
    10981 tgtataaagg acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa
    11041 gaggttttag tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat
    11101 tatacggtgt taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga
    11161 taactcatca gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa
    11221 ataatcaaat acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt
    11281 gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac
    11341 agactgcaca aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg
    11401 aaaaaacact tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag
    11461 aggagcttaa taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct
    11521 tcttcagatt attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga
    11581 aagacataga cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat
    11641 ttggtaaagt tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag
    11701 atactgagac tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa
    11761 aacttggata caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc
    11821 tatacattcc aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc
    11881 ctcgtattac acctcatggg tttagacata cacatgctag tttattatta gaagcaggag
    11941 aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca
    12001 tctatgcaca tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga
    12061 tggctcatca gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa
    12121 aaaagctaga acccttatat atcaagggtt ctagctccta taacaggttt acccaacact
    12181 gccttccatc tcataactga tcaatcgatt gagttcgact gcatattcca ttggaagttc
    12241 tttggtgaat ggttcaacaa agcccatgac gatcatttca gttgcttcag attcggtcag
    12301 gcctcggctc atcagatagt agagttgttc ttctgagatt tttgagactt tggcttcgtg
    12361 ttctaaagag acttggctgt tgtggatctc attgaatggg atcgtatcgg atttcgacca
    12421 ttcgtccatg atgatcgtgt cacattcgat atgagagatc gaacccgcac tgtttttggc
    12481 aaaggttact tgtcctcgat agtttacttc tccgccgtct tttgaaattg atttagagac
    12541 gatagagcta gatgtatttg gtgcattgtg gatcattttt gcacccgtat cttggatttg
    12601 gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt gcaccttttc catctaagta
    12661 aacacttggg tatttcatcg ttgtttttgc acctaggttt ccgtcgatcc attcaacggt
    12721 tgcgccttcg tatgcttttg ctcgtttggt tactaagttg tacacattgt ctgaccagtt
    12781 ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta aagatttcta cgatggcggc
    12841 atgcaggctg ttacttgaat aagtaggtgc cgtacaacct tcgacatagt ggatgcttgc
    12901 tccttcgtca acaatgatca gcgtacgttc gaattgcccc gtgttttccg cattgatccg
    12961 gaagtacgtt tgtaatggaa catcgacttt gacgcctttt ggcacgtaaa taaaggtccc
    13021 acctgaccat acggctgaat tcagcgctgc taatttgttg tcggttggcg gtacaagttt
    13081 tgaaaagtac tctttgaata attcagggta ttctttcaaa gcagagtcag tgtcagtaaa
    13141 gacgattcct aacttttcga attcatcttt catattatgg tacacgactt ctgattcata
    13201 ttgtgcggaa gcaccggcta gataagctcg ttcagcttct ggtataccaa tcttttcaaa
    13261 tgtatctttg attttttctg gtacgtcttc ccagtcacga gcgggtttgt cacttggttt
    13321 ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg tcggggcccc atgtttgcat
    13381 tggcattttg ttaaatgttt ctagagattt cagacggaat tcaagcatcc attctggttc
    13441 gcctttgatt cttgacatct cacggatcac ttcttctgtc aagccttctc ctgtactgaa
    13501 aaccggttcg acatcatcgt gaaacccgaa tttatattct tctaattctg gtacgcccat
    13561 gttctcactc ctcttccttg tggtaatgat ggaaagctgc ggtaccatcg ttttccattg
    13621 cttttcctag tgctttccag gcaagtgtcg cacatttgat ccgagcggga aatttagcta
    13681 caccaccaag catagcagca tcgcctaatt ggtcgggatc tttgacttct tttccttgga
    13741 caagctgtag aaagtctaca gttaaatgtt ctgcttcttc tattgatttt ccgatgacgg
    13801 cgtctgtcat catcgaggca cttgccgtac tgatggagca gccactgccg tcaaaggcga
    13861 tatttttgat gattccgtct tttacatcga cttgcaattc gatcacatct ccacatgtgg
    13921 ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct tccacgatga tgcggatggg
    13981 aagaatgatc caagatcact tgacggtata aattgtctaa tttagatagt cccatgttgg
    14041 aaaaactcct ttgttgccat gattgcttca actaaacggt cggcgtcttg cgttgtgttg
    14101 tagaaataaa agctcgcgcg agcagtcgct gatacttcaa ggtacttcaa tagcggttgc
    14161 gcacaatgat gacctgctct gacggctacc ccttccatgt ctaaagctgt cgcgacatca
    14221 tggggatgta acccatccaa attgaatgca acgactcctg tccgaaaatt cgggtcttgt
    14281 ggaccataaa cagtcaatcc atcaattgct tgtaattttg gcaacacata agcggctagt
    14341 tcttcttcat atcgatggat attgtccata ccgagttttt gcaaataatc gattgcatgg
    14401 cctaatgcga tagcgccggc gatatttggt gtccctgctt cgaatttcca aggtagttct
    14461 ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt cgccaccaaa ttcaacaggt
    14521 tccatttctt ctaaccattt tcgtttacca tataaaacac caatacctgt tggtccgcac
    14581 attttgtgtc cgctgaaagc aaagaaatca cagtctagtg cttggacatc aatggccatg
    14641 tgcggggcag attgagcacc atctactacg atgaccccgc cgttttcatg gacaagttca
    14701 gctaattctt gaacaggatt gaagacacct aagacattgg atacatgagc aatggagacg
    14761 attttggttt tgtccgtaat ctttttcgct gcatcctcca tatccaggaa gccttcttct
    14821 gttagttcga tatatttcaa gacagcacct gttcgttttg ccaactgctg ccacgggata
    14881 acattagaat gatgttccat gtaagagagc actatctcgt ctcctgcttt gatatagcgg
    14941 tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg taccacgagt aaacagcact
    15001 tctgctgttt cttttgcatg gatgaacgcc cgtacttttt ctcttgcttg ttcataatca
    15061 tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg
    15121 taatagtacg ctaatgcctg gagtacttgt tttggctttt gggtcgtcgc tgcattatcc
    15181 agataaacta aaggttcatc attgacgtct tgaaataaga taggaaaatc attgcgtagc
    15241 tttttagcat cgatcatcca ttcaacttcc cttcgattac ttcgactaat tctttccgaa
    15301 cttgttctac agggattgct gttaagacag aaccgaggaa tcctcgaatc actaagcgct
    15361 ctgcttcttc tttatgcaat cctctactca tcagatagta catttcttct ggatcaactc
    15421 gtcctacact ggcagcatgt ccagcagtta cttcgttttc atcgatcaaa agaataggat
    15481 tcgcatcacc acgtgcttta tcagaaagca tcaagacacg actttcttgt tgggcatctg
    15541 caccttttgc ccctttcaag atgtggccga taccattaaa ggtcaatgtt cctttttctc
    15601 ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg ggctttattg gttactcgtg
    15661 tatcgattcc ttggatttgt ctgcccgagc tgattgccac gattttgact tctgcgtgtg
    15721 caccttctcc tgctaaatct gagtcaaagt cagcgatcac atgaccgtca ttcatgacac
    15781 caattgccca gtcgacagaa gcatctcgta aaatgtgccc acggcgattc atataagaag
    15841 taatattttc tcctaattga tctacggctg aatatttgat ttttgaacca gctttagcga
    15901 tgacttccac gatgatatta cctgatgatt ttgctacttg ttctttggtt gtctggaaac
    15961 gttccagata agaaaattca ctatgttcat ctgccacgat caacacatgc ttgaagaagt
    16021 gctcatcgga tgcgccatct tgaatgaata aggactcgag tggttcatcg atcacgacat
    16081 ttttaggcac atacaggaaa ataccgctgt tcataaaagc tacatggagt gcagtcaatt
    16141 ggtcttcgtt catgtttact gcttttttca tataatattc ttggactagt tctggatatt
    16201 cgatcattgc agtgaacaag tcggtaaaaa tgactccttt ttctgctaat tccacaggca
    16261 gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac aggattgtct ttcattgcat
    16321 caaatgccgg aatcgtgcct gtttcactga tggtttgtgt ttctttcaca tcaaataacg
    16381 accagcgatg gaatttcacc cgatcgatat gcggcaatgg cagttcatct gctttcgcta
    16441 gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc tttacttaaa gaaaaatcat
    16501 ttaccgcgtc aagataatcg agccagtttt tttctttcat tcttgctccc ccttacgctt
    16561 cttcttcttt atagtcgatt cctaattctt tactgatacc agcgtagcct tctgcttcca
    16621 gacgtttagc taggtcagca ttccctgtca tgactactcg gccttccatc atgatgtgaa
    16681 cgacatcagg tgtgatatag ttcaataatc gttgatagtg ggtgatgatc aatgcaccga
    16741 aattgtctcc gcgcatttca tttactcctt tagcaacgac tttcaaagca tcgatatcca
    16801 gacctgaatc gatttcgtct aaaattgcaa atgtaggttc taacatcaac aattgcagga
    16861 tctcattccg tttcttttct ccgccagaaa aaccttcgtt cagataacgt tcagccattt
    16921 cttctggcat atttagtaaa gccatttttt catctaactt tttcaaaaat gccataacag
    16981 aaattttgtc gttttcatca cgtttcgcat tgatcgctgc acgcatgaat tccgcattcg
    17041 tgatacctgg tatctcactt ggatattgca tagctaaaaa taagccaagt cgcgcacgtt
    17101 catctacttc caagtctaaa acatttttcc cgtcaaacaa aatttcgcct tctgttactt
    17161 catagtttgg attgcccatg atagcggcag ataaggtaga ttttcctgtg ccgttaggtc
    17221 ccatgatggc atggatttct ccggttttca ttgttaggtt tacgccttta aggatttctt
    17281 tttcctcgat cgacacatgt agatttttta tttctaagac agacatgcat tataccccca
    17341 aattctcttt cgttctcact cattttagac taattgagaa tgaagcgcaa tgaccttcct
    17401 tactaaaatc aaatatcttt tatttattat taaacaattt tgatttctaa attagaatag
    17461 gtataaaaaa gatgagaaat aagccattac agacttattt ctcatctttt ttgttccata
    17521 ccttattttg catcagcctt tactggttta acagagccgc cccatttgtc tgtgatccat
    17581 tcttggactt ctttgcggtg aagcacttca atcagttttt tgattttgtc actgtcttca
    17641 tcacctttac gagtcgcaat aatattcacg taaggagagt tgtccttttc aagcaatacg
    17701 gcatcttcta atggattcaa ccctgcgccg taagcgaagt ttgcgttgat tgcaactagg
    17761 tcaccttcat cattttggta cgtagaagct aaaagtgatg ggtcgatcgt atggttgaac
    17821 ttcaagttct tagggttttc ttcaatatca tcgaatgttg ctgtttccag atctactcca
    17881 tctttgactt tgactaagcc agcatcttgt agaattgtga tgatgcgtcc ccagtctgat
    17941 tcagaatttg aagtaatgat tgttgcacca tcttttaatt cggaaacatc tttgactttt
    18001 ttagagtaca agcccattgg ttcgatgtgg acagcaccgg cattgacaaa gtcatatcct
    18061 ttttctttga tttctttgtt caaataagga atatgctgga agtaattggc atcaatgtcg
    18121 ccttcttcca aagattggtt cggtaataca tagtcatcga atttgacgat ttcaagatcg
    18181 attccttcat ctttcaataa tggtttcaca tgttccaaaa tctctgcgtg cggtgtcggt
    18241 gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg ctgaatcttt gctgtcgctg
    18301 ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg ctgctgcaaa tccaaaaatt
    18361 ttctttttca tatcctacat ccccctatat cctattatct tttgtctgtt ttcttagtta
    18421 agaaatcacc gattccttga acgataaata cgattagtaa aataattaaa gtcgcaacga
    18481 atgtcactgt tagattacca cgctggtagc cttcctgcca agcaagacct ccaagtccac
    18541 ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat cgagatcgtt gtaactgtta
    18601 atccagaaat caatgcggga atactttcag gtatcaagac cttccagatg atttcccagt
    18661 aacttgctcc cattgcatct gctgcttcca gtacaccagg gctgacttca cgaaaagcaa
    18721 tctctactag acgggcataa aatggtgctg cggataaaac aagagctgga atggctgctt
    18781 gcgcacctag catagtgcct acgattgctt tagtaaatgg aatgatcaaa accatcagga
    18841 tcataaatgg cgttgaccgg aaaatattac tgatgatcga tacgattcca taaatgattc
    18901 gagcatacgg tttgtttttg cgtccaatag agtaaaggat caatcccaaa actaatccga
    18961 tgatcgtcac taagaccatt gagattaatg tcatatatag tgtatcaagg atagcctgat
    19021 tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt ttctgaaaag cttttatcca
    19081 cgatggatca cctcaattcc tactccttgt tctttgatga acacttctgc ttgatctaat
    19141 tctttggttt caccagtcat caagacagtc aatgaaccaa aggaaccttc tttcacttgt
    19201 ttgatctttc catacacaac gttgacatcg atgttatagc gtcgaatcgc ttgggaaatg
    19261 actggctcgt tggcatttct ttcgctaaag gtcaaagttg ccaaacggcc gcttggattt
    19321 tctttgatga gttcagctaa tagttctgtc gaatcagctt ctggttcgat gtcttgttgt
    19381 acaaagcgtt tggttacttc ttttttcggt tgtctgaaga cagtcaatac gtctccttct
    19441 tctacgactt tccccaactc catcacagcg actttgttgc agattttgcg gatcacattc
    19501 atttcatgtg tgatcaagac gatggtcaaa ttcaatttct tattgatcgc taacaataag
    19561 tctaatactt cttctgttgt ttgcgggtcc aatgcgctag tagcctcatc acaaagcagg
    19621 atctcaggat catttgccaa tgctcgtgcg ataccgacgc gttgtttctg tccgccggaa
    19681 agttgtgaag gatatgcttg tccgcgtcct tctaacccga ctaaacgcaa cagttcttct
    19741 gcccgttctt tacgtttgct ctttggtacg ccagctaatt ccagcggcag ctggatattt
    19801 tcaagtacag ttcttgacca taacaggttg aaatgctgga agatcatacc gattttcttt
    19861 cggaatgtac gtaattcttt gttttttaat tttgtaatat cttgatcgtt tacgatcact
    19921 tcgccgctcg tgggaagctc aagtccattc aggagacgga ctaatgtact ttttcctgcg
    19981 ccagaatagc cgacgattcc ataaatatcc cctttttcaa cgtttagtga aacgtcattg
    20041 acggcttgga tgtttccgtt tttcccattg aatgtctttt tgacattcgt tagttggatt
    20101 aatgacatgc cattcacatc tttctcttat aatttttcaa acaaaaaagc cttcgccctc
    20161 ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt accaccttga tttaccgtta
    20221 cttcgcaata acagcctctt ccagtacttg gacttagctt ctttccgtat actgtcgcgc
    20281 tgtaacgggc actcccgtag taagtttgct tacgctcgct tactctgctc taagaccatc
    20341 ttccatctca ttttcgttac ccattttcag caaacagggc tctcttcaaa cgtccgtgac
    20401 atgtactctt cttttcaaag ctttctcgtt tcatatagga ttgcttatga ttatagcaac
    20461 tggaagaatc agtgtcaact tttttcaagt gattttctga ttttataatt cgtcaaattc
    20521 ttttggatca acatctttga aacttaagat ccacgcattc atttcatctg aatcattcaa
    20581 gatattgata tcttcaccga ttttatcatt aacagaagaa actgtacccg ttagcgggct
    20641 ggcaaattca ttgaccgctt tttccgcttc aacttcgatc agaggttcat ctttttttag
    20701 cgattgtcct gcttttggca gattggcaaa cgtgatgtct ccaagttctt cttgcgcttc
    20761 atttgtcaaa ccaacacagt attcttttcc attaaataag atccaaaggt tgtccttttt
    20821 cttcaaatgc ttgcctgcca ttttatatca gttctccttc ataaattttt tcattgaatc
    20881 caattgcaac aagctggtcc tctcgtaaga tcagtggtcg cttgatcagc ataccatctg
    20941 tcgataagac ttggctcgct tttgaagccg taaactcgcc tacttgatct ttcagtccaa
    21001 gttcccgata tttcatcccg cttgtgttga acaggcgacg gattggtact tgactcgttt
    21061 ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt gatcatatca acggtttgat
    21121 aactaacgcc atgctggtct aaccacgctt tggctttttt gcatgttgaa catttcgggt
    21181 accagtaaaa ggtgtacaat tttcttacct ccttgaagat cttgcaggtg cttcattttg
    21241 gtatctcatc gataatatca acccgatacc gatcatgttc cctaaaatcg aggatcctcc
    21301 ctgactaatg aaaggcaatg ggatccctgt taaaggaagt aatccgatat ttgctccaat
    21361 attctcaaag acatggaaca gcagcatcat gatcaaaccg cttgcgatat aggcgtaaaa
    21421 ttcattattc gtatcgaaac acacccgaat catacgatag attaaaataa aatacaagaa
    21481 aatgacgaat gcaccaccga taaagccgaa gttttcaccg ataacggtaa aaatcatatc
    21541 ggactcacga acaggcacat agatattgct tacgttgaat cctttaccaa acaagccacc
    21601 ggtaccaatt gccaaaagtc cttgggccgg ctgataactc attcctgacg tatcatggaa
    21661 agggtccagc catgaatcaa tacgtgcaaa ctgataagat ttgaatccaa ctttataaag
    21721 caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt cctgctccaa cgactactgc
    21781 caagattact acaggtgcga tgatgcgcca agagattcca gacatcagga aaatcccgcc
    21841 aaagatcgca agaaagacaa gcatcgttcc aaagtcatct tgggccatga tcaatgctaa
    21901 aaccggtatt gtcacgatca agagcttccc aatcagccaa aagtccgatt tcatcgtccg
    21961 ttcttttact tgcgtattat gtttagttac gatcagtgcc atcatcaaga tgtacgcaat
    22021 cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat cgaaaccagt ttttagaccc
    22081 tgttgaagca gctaaattct gatcatagaa tttgagcaag agtcccatta ccactaaccc
    22141 caaaccataa aggtaaggag tcagtttcca gatccatttg gaattcaaat gcatgatgac
    22201 aacgattgct gccgctccaa ctaaatacca gacaacttgc atccctacgc ctttcaagac
    22261 tgctggattg ttcggatcat ggctaagtgc tacatataat gagagcagac caatcaaaca
    22321 taacagaaag actggtaaaa tcacgccata atcaatacga ttatcgttcg aagcttttaa
    22381 ttttttttcc atgtttcatc ccttttctta aaaaccatac tgctgattat tatagcggtc
    22441 cagttagata atggaaagtc tttccataga catttgggaa aaattaactt tccgtttgtg
    22501 cagttgcttg agcaaatgca gtacaaaagc ctactgctta tctttagtgt atcatagaac
    22561 tttttttgat acatgcaatt atctaccagc tgagattctc ctcgctgtga gaagtttctg
    22621 tgctttcatt gtatcgaagg atctcttcgg caatctctag cagctgatca gaaaccagat
    22681 tggttcgttg gatgaaataa aaatatcgat ggaaattcaa cggagtcatt ggcacatttt
    22741 ctggaacaga gcgcttagac aaaatcgagc gaccgattcc ttccttcagc aacgcaacaa
    22801 ttacatcgtt acttttcacg atcatcttct tatcttggat attcatctct tcaaaataac
    22861 gctgcgtgta atgaaaaaca ccagagtttg cttctcttac aagccacaat ttgctttttg
    22921 ggtccccagc gatgactagc tggtcttcca tcaaagagta tctttgtacg ccggaagtct
    22981 ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt cgctacttgt tctaatgatt
    23041 catgagaatt cgtgatttcg atctcaaaat gcactttagg aaaccttttg atcagatgtt
    23101 tcatcaactg aggcaaaaga tagacagcaa aggtgtggga agcaacgatc cgacatactt
    23161 cttcttcatt ttgttcattg agcaattttt gctgagtttc ttcccattct tctaacaaaa
    23221 ccagtgtccg ttcatataat aagtcggctt gcttagttgg aagcatctct tttcttccgt
    23281 tacgttgaaa aagatcgata ttcaattccc gctccaactg tttgatttga ttcgagactg
    23341 ctggttgaga taaaaacagt acttctgctg ctctggaaaa atttcttgtt tcatatactg
    23401 ctcgaaaagt cgttaataac ttaaacatgg caccacttcc ataacttttt atgataatta
    23461 ctataaaaac tatttgattt aaatatacaa aaaaccatct ataatagaaa tgtacaacaa
    23521 aacagttgct gatccaacta gtaacttaga tgaagacaat cagctgtctg cttgctcgta
    23581 atcatacatt cctattcatt ttacaatgta ttcgttcatc ttggtactaa aatctattaa
//
LOCUS       DACCWV010000030        23466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCWV010000030 DACCWV010000000
VERSION     DACCWV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.030"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            48..1709
                     /locus_tag="IVW84_001057"
     CDS             48..1709
                     /locus_tag="IVW84_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7917266.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1779..2126)
                     /locus_tag="IVW84_001058"
     CDS             complement(1779..2126)
                     /locus_tag="IVW84_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7917267.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2210..3151
                     /locus_tag="IVW84_001059"
     CDS             2210..3151
                     /locus_tag="IVW84_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917268.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3152..4195
                     /locus_tag="IVW84_001060"
     CDS             3152..4195
                     /locus_tag="IVW84_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917269.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4201..5271
                     /locus_tag="IVW84_001061"
     CDS             4201..5271
                     /locus_tag="IVW84_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917270.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5261..6214
                     /locus_tag="IVW84_001062"
     CDS             5261..6214
                     /locus_tag="IVW84_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917271.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6226..7236
                     /locus_tag="IVW84_001063"
     CDS             6226..7236
                     /locus_tag="IVW84_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7917272.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7521..8342)
                     /locus_tag="IVW84_001064"
     CDS             complement(7521..8342)
                     /locus_tag="IVW84_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7917273.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8439..8717)
                     /locus_tag="IVW84_001065"
     CDS             complement(8439..8717)
                     /locus_tag="IVW84_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917274.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8985..10994
                     /gene="metG"
                     /locus_tag="IVW84_001066"
     CDS             8985..10994
                     /gene="metG"
                     /locus_tag="IVW84_001066"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7917275.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11473..12060)
                     /locus_tag="IVW84_001067"
     CDS             complement(11473..12060)
                     /locus_tag="IVW84_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917276.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12235..13557
                     /locus_tag="IVW84_001068"
     CDS             12235..13557
                     /locus_tag="IVW84_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7917277.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13712..14488
                     /locus_tag="IVW84_001069"
     CDS             13712..14488
                     /locus_tag="IVW84_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7917278.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14481..15050
                     /gene="rnmV"
                     /locus_tag="IVW84_001070"
     CDS             14481..15050
                     /gene="rnmV"
                     /locus_tag="IVW84_001070"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7917279.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15043..15927
                     /gene="rsmA"
                     /locus_tag="IVW84_001071"
     CDS             15043..15927
                     /gene="rsmA"
                     /locus_tag="IVW84_001071"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7917280.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16378..17007
                     /locus_tag="IVW84_001072"
     CDS             16378..17007
                     /locus_tag="IVW84_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917281.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17131..18240)
                     /gene="ugpC"
                     /locus_tag="IVW84_001073"
     CDS             complement(17131..18240)
                     /gene="ugpC"
                     /locus_tag="IVW84_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7917282.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18449..18871
                     /gene="mgsA"
                     /locus_tag="IVW84_001074"
     CDS             18449..18871
                     /gene="mgsA"
                     /locus_tag="IVW84_001074"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7917283.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19194..19730
                     /locus_tag="IVW84_001075"
     CDS             19194..19730
                     /locus_tag="IVW84_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7917284.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19770..21533)
                     /locus_tag="IVW84_001076"
     CDS             complement(19770..21533)
                     /locus_tag="IVW84_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917285.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21536..23251)
                     /locus_tag="IVW84_001077"
     CDS             complement(21536..23251)
                     /locus_tag="IVW84_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917286.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7557 a         4299 c         4776 g         6834 t
ORIGIN      
        1 cagaaaatat taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat
       61 ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt
      121 cagccacaag cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact
      181 tagtcgtgcc tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt
      241 ttacagcatt gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac
      301 agccggcagg agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca
      361 aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt
      421 atggctggca acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc
      481 cagttgaaaa tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca
      541 aagcagttgg tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat
      601 atctgcttgc tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg
      661 atgcctatgc atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt
      721 ttgatggacc aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca
      781 aagatacagt aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat
      841 tgaacttatt caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc
      901 aaaacgcaaa cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat
      961 tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt
     1021 attctatcga tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta
     1081 caggattgat cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag
     1141 aagctggaaa attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga
     1201 aaaaagaatt gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat
     1261 cgaaaaaagt aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag
     1321 tcaaaccaac acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg
     1381 acgcagtact aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt
     1441 ttgtcacagg taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag
     1501 ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa
     1561 aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag
     1621 gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg
     1681 attacaaatg gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga
     1741 ggctgcggca aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc
     1801 atgaaaatag cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata
     1861 ccgtagctgg cagacgagag cgtggaaaat tctggttttt tctttcggtt ccttgttcgt
     1921 gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa
     1981 ccgccgataa tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt
     2041 gtaaaggaat ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact
     2101 gcgtagggga tagatttttt tttcatttga ttgctccttt atttttggtt cattatctaa
     2161 taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag tgaatagtta
     2221 tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt taattgcaac
     2281 aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc aggaacgttt
     2341 gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac cagttatcgt
     2401 acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt ccttccaatt
     2461 taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct tgcagttagg
     2521 gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga tctctgccat
     2581 gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag gtcgttccat
     2641 tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat tacaagtttt
     2701 gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg cgttggccat
     2761 gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag tactgcatag
     2821 tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg cctttaaaca
     2881 tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag ctgtagcatt
     2941 gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg gggaacaatt
     3001 cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga tcctttactc
     3061 atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag ttgatccaag
     3121 aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc gttatgaaac
     3181 agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg aagaagaacg
     3241 tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc gtttaaagaa
     3301 aaacaaagct gccgttattt caatggtttt actattggtc atcattttga tctctgtgat
     3361 cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata tcaatttgcc
     3421 tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg ttggtggtga
     3481 acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct ttttaggaac
     3541 agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa tctcgttgct
     3601 gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg ggttggtttc
     3661 tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa tcttatcagg
     3721 aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag gaattttttc
     3781 aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca
     3841 aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag gtgaatcaag
     3901 aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta ttattcaaat
     3961 gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta tcggtttagg
     4021 tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca agacattcct
     4081 gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca tgattggctt
     4141 taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg
     4201 atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta tgctggaaag
     4261 gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt ggcgatcgtt
     4321 ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt gctgtcaagc
     4381 aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt tggaaaatca
     4441 gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca agatccaatg
     4501 acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct tagaaagcat
     4561 aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa attagttgga
     4621 atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg aggtcagcgt
     4681 caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat tgctgatgaa
     4741 cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct gaaagaaatt
     4801 cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt agtagcgaat
     4861 gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg aacatcggaa
     4921 gaaattttct acaacccgca acatccctat acttggggac tgcttggttc aatgccgaca
     4981 ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga tttattgaac
     5041 ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa
     5101 caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg gctacttgct
     5161 ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc tatctttaaa
     5221 gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata aactacttga
     5281 agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa
     5341 tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag aatctggaag
     5401 cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg atggtgagat
     5461 attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga cgaaattccg
     5521 gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt
     5581 cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat ctgaagagcg
     5641 tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc acggtactcg
     5701 ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac gagcattggc
     5761 agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg tgtcgatcca
     5821 agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga cctatctatt
     5881 tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg taatgcataa
     5941 cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg ttcatcctta
     6001 tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga gaaaacgcag
     6061 aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc gtgaaatcgc
     6121 accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa aaaaactaaa
     6181 acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct aaaagctttt
     6241 aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac ttttggatgt
     6301 gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca acaaacagga
     6361 gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt cttaaaagaa
     6421 gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt tcgcaattat
     6481 ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat
     6541 acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa gttaccaaaa
     6601 caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat tcgctccgct
     6661 acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga tgtttcgcca
     6721 gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa gacattgatc
     6781 caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga agaaaagctg
     6841 caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg
     6901 ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct ttatctgaaa
     6961 caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt caaacaatat
     7021 gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt ttctattcac
     7081 gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa taaaaaattt
     7141 cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag ccaagtagta
     7201 gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac tgataggagc
     7261 gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg aatttttctt
     7321 ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa aagttcgtac
     7381 gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct
     7441 gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc
     7501 tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa
     7561 ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc
     7621 ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt
     7681 cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc
     7741 attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa
     7801 gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta
     7861 gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca
     7921 tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag
     7981 cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa
     8041 ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc
     8101 ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg
     8161 atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca
     8221 gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct
     8281 tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc
     8341 atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa
     8401 aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa aataaatgga
     8461 agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata ccgctcttca
     8521 cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg caaaatccaa
     8581 gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata catacgaata
     8641 taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa
     8701 agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc tttattatac
     8761 cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc
     8821 cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat agaagtcgtt
     8881 tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat ggtatactag
     8941 caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa
     9001 ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc ggcaactcgt
     9061 atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc tttgatgtgt
     9121 tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca gaagaactag
     9181 gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa
     9241 cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat aaagcagctg
     9301 ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt gaatatgaag
     9361 gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca gaagtgtaca
     9421 aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa gtagaattgg
     9481 taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta ttggaatact
     9541 atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg atcaacaact
     9601 ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact tggggagtac
     9661 cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta tccaattata
     9721 ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac tggcctgcag
     9781 atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg ccaatcatgc
     9841 tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg ctattgatga
     9901 aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac
     9961 gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt gggtcagatg
    10021 gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca aatgatctag
    10081 ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga ctcgttcctg
    10141 actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct gctaacgttg
    10201 tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg gctgaaatct
    10261 gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg gtattagcaa
    10321 aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct gaatctttgc
    10381 gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag atcttcaatc
    10441 aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc gaatttcctt
    10501 caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat atggaagaag
    10561 aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa gatacagtca
    10621 aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc aaatttgatg
    10681 tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg
    10741 cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt caaatcttgt
    10801 caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg
    10861 tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg atcctttctg
    10921 cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct
    10981 cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac cccctttttc
    11041 cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca atcatataat
    11101 acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt
    11161 tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag cataatgata
    11221 aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc cttacattca
    11281 taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt agcgttgatg
    11341 tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat atgtgtgatt
    11401 gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt tctttatttt
    11461 gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca aacggcttgg
    11521 taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc
    11581 gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca accctaaaag
    11641 caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa gtgccatcac
    11701 tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt tctttgtaaa
    11761 tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag
    11821 cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt tgatcaatgc
    11881 aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg caacagtcat
    11941 taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt ctttacggga
    12001 aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct taaaatccat
    12061 aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa aaagaaatac
    12121 atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt aaattaaaat
    12181 attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa
    12241 ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat gggaaccgtg
    12301 atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca gtctacaagt
    12361 ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg attgacagtg
    12421 gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat tgaagctgcg
    12481 tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta ctttcctgct
    12541 aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt acaattatct
    12601 accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg gttgaatttg
    12661 ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa attaatacca
    12721 attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt ccagttattt
    12781 ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc gttgttagca
    12841 acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg
    12901 ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac agtcgtttat
    12961 ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc cggtaatttg
    13021 aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa gctggtcact
    13081 attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac tgggatccgc
    13141 gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct gacaaagctt
    13201 tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt agcttgtatt
    13261 atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt gatgtggcta
    13321 ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca
    13381 cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct cggtgctatt
    13441 ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg tataggtgtt
    13501 accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat
    13561 atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt gctttttcta
    13621 tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta gtgcttatgt
    13681 gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata cccatttaaa
    13741 tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag aaatgggggt
    13801 aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt tgcaattaag
    13861 ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg ctggcagtta
    13921 tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg ttgtagcatt
    13981 aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc aagataaagt
    14041 atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta ttcatactag
    14101 agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag atatcggtgg
    14161 aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg acttagggat
    14221 gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc
    14281 agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc tggcaccagt
    14341 tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag aaaagattgc
    14401 tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa atgcgcaccg
    14461 attatttggt ctaagcgaac atggataaga tgaagatcca agagattata gtcgttgaag
    14521 gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata gaaacaatag
    14581 ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag actcgtggag
    14641 tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc attatggaag
    14701 ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca aagaaaaagg
    14761 gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg
    14821 tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac cttttagact
    14881 atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt gacgagttgc
    14941 gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt cgtattacgc
    15001 cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga atatcgtgaa
    15061 attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc atttaaaaaa
    15121 agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt agaaactgcg
    15181 caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc attgacggaa
    15241 caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg tttgatccct
    15301 gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca agatatttta
    15361 aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc gttaaaagtc
    15421 gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct tgaatcagat
    15481 ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg tatctctgct
    15541 gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta tatggaagcc
    15601 agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt agattcagcg
    15661 attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga aaaagcattt
    15721 ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa taacttgcag
    15781 cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt ggaaacagct
    15841 ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc cgcattaagc
    15901 aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc
    15961 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg
    16021 tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc
    16081 ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc atgtttttga
    16141 gttagttggt cttattactt gatgcaagct tttgaccact attatgccga acaaggagat
    16201 gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat tcttatactt
    16261 gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac agttattagt
    16321 aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag agaaaaaatg
    16381 agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat gggggtcatt
    16441 atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt ggcgtctcat
    16501 gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt tgcgctaggt
    16561 acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc attgtcacat
    16621 cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa
    16681 aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt gatcggtgtg
    16741 attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg taatatgcct
    16801 gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg gattggtggg
    16861 cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac actcagcaaa
    16921 tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa
    16981 gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac tctcatctag
    17041 taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc tgcaccaaca
    17101 tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt gttctgtttc
    17161 tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt cgcctggttt
    17221 atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt ttgtgtaaag
    17281 catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta cggcttcagg
    17341 tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac cgaagataac
    17401 ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag gaatacgaag
    17461 cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa agaagttcat
    17521 ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat aaacttcttt
    17581 cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt cagccatagt
    17641 cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac gctggtgaag
    17701 tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg ataatggctc
    17761 atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca cacgttggcg
    17821 ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac ctaagatctc
    17881 tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta atttcaagcc
    17941 aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt ggaaaaccat
    18001 ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga tatttagttc
    18061 tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact ttccacaacc
    18121 ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa aatcagttac
    18181 agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca tttctaccat
    18241 aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa atgaaagcgt
    18301 taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca atgtgctagt
    18361 tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt aaataagtat
    18421 gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa
    18481 aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa aacatgaact
    18541 ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc cgatccatcg
    18601 gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt cggaggatca
    18661 gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg aaccagatgt
    18721 aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga atatcggaac
    18781 agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa gaatcgtcca
    18841 tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa ataacgttta
    18901 atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga tataatacga
    18961 acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa atgtgaaaat
    19021 atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa ataaagcgtt
    19081 aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc gcggttttat
    19141 tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta aaaatgaaga
    19201 aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca ttgatggtca
    19261 ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc acatttattc
    19321 cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc gcagttttag
    19381 ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga tttacattga
    19441 atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag atcacttgga
    19501 aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt ttgacaccat
    19561 tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc
    19621 tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac gtaggacgta
    19681 gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa aaaacaagca
    19741 gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc ttgatagagc
    19801 cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc gacaatagct
    19861 ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg atgggcaatc
    19921 acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt tttctctgtc
    19981 aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt actgatcatc
    20041 gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc ttgaccgcca
    20101 atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata agaggctttt
    20161 agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag attttctcgc
    20221 aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt acgtaatgta
    20281 tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt tgttgtgtca
    20341 taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt tcctactata
    20401 gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg ctgcttgggg
    20461 tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt gggttccaag
    20521 gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc aaatgctcgt
    20581 tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc gataaatggt
    20641 ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt gattgacgaa
    20701 ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc aaggtgatct
    20761 acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg tcctttttca
    20821 tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg ctgaaaagct
    20881 ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc cccaataatc
    20941 ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact cacaaggaaa
    21001 ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg catcataaaa
    21061 ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc cgcagaaatt
    21121 gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc gtgggagttt
    21181 tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt tcttaatttt
    21241 gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg actgactacg
    21301 ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt gaagcggacc
    21361 tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat cacaactgtt
    21421 gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc ataggcagta
    21481 aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt cataactatt
    21541 cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt aattcttcat
    21601 gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt gcttcttgaa
    21661 tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc tgtagcgcct
    21721 tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct aaaatcagta
    21781 atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt ccgccagaga
    21841 aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga cggacaaatt
    21901 cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg tctttcttac
    21961 cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg ggaacaagag
    22021 caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg tccattataa
    22081 ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc gttttcccgc
    22141 ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat gtaatatttt
    22201 ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa gcgataattg
    22261 cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa ttttctgtgt
    22321 ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc acgacgaggt
    22381 ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa gcaagaactt
    22441 gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc aaacagatga
    22501 tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata ttggaaacac
    22561 gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt tctttttttg
    22621 tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg tgttcattga
    22681 gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg atgataaaaa
    22741 ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact tcccagtcga
    22801 tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc aataaacgaa
    22861 tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg gtgatcaagg
    22921 tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt ttgattagct
    22981 gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat tggcaaatga
    23041 tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt tcaatgattt
    23101 ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt aaaggcagga
    23161 caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt tgcttacgat
    23221 agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt atttgtccac
    23281 aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga aaagagaaca
    23341 agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa tagactattt
    23401 tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg aacgtctatt
    23461 ttactt
//
LOCUS       DACCWV010000031        23392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCWV010000031 DACCWV010000000
VERSION     DACCWV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.031"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..2181)
                     /locus_tag="IVW84_001078"
     CDS             complement(37..2181)
                     /locus_tag="IVW84_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7917287.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2538..3911)
                     /locus_tag="IVW84_001079"
     CDS             complement(2538..3911)
                     /locus_tag="IVW84_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7917288.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3911..4306)
                     /gene="rbsD"
                     /locus_tag="IVW84_001080"
     CDS             complement(3911..4306)
                     /gene="rbsD"
                     /locus_tag="IVW84_001080"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7917289.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4334..5251)
                     /gene="rbsK"
                     /locus_tag="IVW84_001081"
     CDS             complement(4334..5251)
                     /gene="rbsK"
                     /locus_tag="IVW84_001081"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7917290.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5359..6372)
                     /locus_tag="IVW84_001082"
     CDS             complement(5359..6372)
                     /locus_tag="IVW84_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917291.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6453..7052)
                     /locus_tag="IVW84_001083"
     CDS             complement(6453..7052)
                     /locus_tag="IVW84_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7917292.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7238..7939
                     /locus_tag="IVW84_001084"
     CDS             7238..7939
                     /locus_tag="IVW84_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917293.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7949..10660
                     /locus_tag="IVW84_001085"
     CDS             7949..10660
                     /locus_tag="IVW84_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7917294.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11111..12739
                     /gene="treC"
                     /locus_tag="IVW84_001086"
     CDS             11111..12739
                     /gene="treC"
                     /locus_tag="IVW84_001086"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7917295.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12799..13980)
                     /locus_tag="IVW84_001087"
     CDS             complement(12799..13980)
                     /locus_tag="IVW84_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7917296.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14232..15755
                     /locus_tag="IVW84_001088"
     CDS             14232..15755
                     /locus_tag="IVW84_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7917297.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15824..16552)
                     /locus_tag="IVW84_001089"
     CDS             complement(15824..16552)
                     /locus_tag="IVW84_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917298.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16565..17845)
                     /locus_tag="IVW84_001090"
     CDS             complement(16565..17845)
                     /locus_tag="IVW84_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7917299.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17986..18588)
                     /locus_tag="IVW84_001091"
     CDS             complement(17986..18588)
                     /locus_tag="IVW84_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7917300.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18833..19039
                     /locus_tag="IVW84_001092"
     CDS             18833..19039
                     /locus_tag="IVW84_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917301.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19351..20070
                     /locus_tag="IVW84_001093"
     CDS             19351..20070
                     /locus_tag="IVW84_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7917302.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20151..20834)
                     /locus_tag="IVW84_001094"
     CDS             complement(20151..20834)
                     /locus_tag="IVW84_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7917303.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21053..23239
                     /locus_tag="IVW84_001095"
     CDS             21053..23239
                     /locus_tag="IVW84_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7917304.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7781 a         4213 c         4589 g         6809 t
ORIGIN      
        1 gagtgtgaca gtgtcacatc ccctttttaa tttattctat ttctttaaag gcagatcaag
       61 ccagaatgat ttgtctaaac ttaacaaggt tagtacagga acagttgcag ctttaactgt
      121 acgagtagtt gtttctggta tctgtcttgc cgtaaaattg ttccacaaat cccagattga
      181 agcgacacgg aagatggaag aagcgttgtt gacgcgtttt atcgttgaaa attctaatcc
      241 tgtgattggc acgccatctg ttgtccattt gattggcaca tcgaatcgct tagacatatt
      301 gaggatttca gcgtttgctt cttcagttgt tgctgcttct tttttcggtt catcatattt
      361 tgtcaggtcg tacgcttcga tgatcgcgtt caatttttgt gaatattggc gatctgtagc
      421 ataacgtcct tccaaatatt tggttgcttc tttgtagtct tttgtttccg acttccaagt
      481 tggtttgtag aaatcaggat tgccagtcag tccatttttg atcaagtcgg catagtcttc
      541 caaactttcc ttgtaagaag gatatttacg gaattgtgcg cggatttggt agctttgtcc
      601 ggaactatct tgttcaagtg tattgaaggt cacgctgctt cctttatatg atcctttgat
      661 accaaaaagg ttatgatttg gttcgcttga taataagctg tttccagatc cactttcaag
      721 gatagcttga gccatcatga ctgatgcgta gattccttct ttttgcccga tttcctgtgc
      781 atcatctgca atctttttca agaactgatc agttgtttgg ttttgactga aatggaacga
      841 gccatctaat ccatctaaaa tagatgagcc gcctgtcagc gaacctgaag cagcaggtaa
      901 acctgctaag tttactcgga cagcaaaatc aggcgtgaaa tctgaaatag aagttactcg
      961 aactttatct cctggttgag gcgcatggat aaattgtccg ttcccaatat agatacctac
     1021 atggtgagta acaccggaag aaggtcccca gaagacgagg tcgccaggtt gggcctgact
     1081 tactggaatc tgcgtacctg catattgctg ttcgcctgtc cagccaccga tataatgacc
     1141 ggttgctttc atatatacgt aataagtcaa accagaacag tcaaaagtat ttggaccttt
     1201 tgctccccat acatatggtt ttccaagatg cttgaatgct tctcgaacga ttgcttgttg
     1261 gacattcgtt gtatttgtag gcaatacagc atcaattggt gattttacaa caaaatcaga
     1321 agaagcagaa gatggattct ctataccgct ttgatttgct gtatttggac gagatgaagc
     1381 attgttcgtt ccgccattcg atgaaccatt gtttggatgg tttggttctc tctgagtatt
     1441 tccattgtta ttcgatccag atgggtttgt ttgattcgaa gaattcccgc tgttcgaatt
     1501 attgttatta ttgtttgaat tatttgaacc attattattg ttgttattac ccacatctgg
     1561 tttgtttggc gtttccggat ctggtgtact agggacttct tcactaggat cagttggttt
     1621 ctctgttgaa gtatccggtc cagctttaaa ttctgcagta atagataaat tgttcgtacc
     1681 tactgtaaag gtctttgcat cataatttac ttctttggca ccattaacag cgactgaagc
     1741 aatttgttca ccttcagctg gcgtaatagt gtaagttacc ttcgtttccg ctaataaacc
     1801 ttctaccgtt ccactttccg taatatccaa aggttgctgt tcaggggaat ccgctgtcga
     1861 agcagtcagt tggatcttac caatcaaaga aggagcaatt gtaagggaat atgtctcggc
     1921 tggcttttca ttttctgatg gatcagccgg atcaatttca gttggctcgg tcgatcctgt
     1981 tgttgagtta gttgttccag ttgggtcttc tgtaaaagaa tccgttgttt cactagtagc
     2041 tgagctggat gtagtcgtat ctgttgtgac tgtttgatca gctatttgat tatctacggc
     2101 aaatacggta ttggtcgaca aaacagacgg agcaatcagc caagtagcag ctagcaaacg
     2161 tatcgttttt ttatttttca taataaacta gtccttttcc tttcattgat tttctgtctt
     2221 attcagcatt cattagacat tttttggaac agataatcgt gtcgaaaagc tttttctttt
     2281 cataaatctg tctgtgtatt ctaaataaaa caatttgtaa ataagcaacg agcattcatt
     2341 cgtttttctt tgacgttcat tttattgcat agaccacctt tgtttgggga taaacaagtg
     2401 gggataaaaa tggttatgca tatgtaaatt ataccaaagt ttggaaagta tcacaaaaat
     2461 ttataaaaat aatgatccta aaaagaaaaa gactgaaata tgtgataaac atctcatact
     2521 tcagtctttt tcattaacta ttcataagac attaggtctt catcaaaccc gctaacgaga
     2581 gtagtgatca ttgctacgac tagactaaca gccagtcctg ctaagtacca gaaaaaagta
     2641 ccggaaatga atgcaggcaa tgtggtcaga ctaccaaata caaaagcgat cgctttcact
     2701 tgggcaaatc ccattacagc tccaccagca gctgcaccaa tgcaagcagc aacaaatggt
     2761 tttttcaatg ggatcgcaat tccgtaaaca gttggttcgg tcactcccgc aagtaaaatc
     2821 gaagcaaaag cagaaccgga gatcgaacgg gtttttgaac gtttacttcg cagaaataca
     2881 cctaaagctg caccagccat tccaaaattg gctgctcctg tcaacggaac gattcggtcg
     2941 aacccataag cagagatatt actgatcatg acagggttta cagcccattg gataccaaat
     3001 attaccaaaa tattccaacc accagcgatg attgcgccac tgatcggtcc gttaatatcc
     3061 atcatccaag taattgcggc agctaaacct tcgccaaggt aaacacccgc aggcccgaat
     3121 accattaacg tcaaaggaac cgtgataact aatgaaagta aaggtacaaa tactaattgc
     3181 atattttcgc gaatatattt tttgaggaat ttttcaacaa aagaataaaa gaaaatacct
     3241 aaaatcgccg gcatgacagt agacgtatac tgcatcagta ctacaggaat ccccagaaaa
     3301 tcagtagctg ctttgccact gtctgaaagt aaagaggtca gattcggttc aatcagtgct
     3361 gcagcaatga cagccgcaat atatccattc actttaaacg ttttggcagc agaaaaagca
     3421 agaaagattg gcagaaaata aaagacggca ttcgacgcag cagacaaaat atgataagtt
     3481 ccgcttgttt cactaagcca gccaatcgta gtcgctaaaa caaccagacc ttttaatata
     3541 ccagaaccgg caaacagcgg aatgatcggc gtaaagattt ccgatatcaa accaaatagt
     3601 ttttcgatcg gatttagctt ttctttcgga gctgaatgat cgatttgacg cgtcaattcc
     3661 ttgtaagcag gagccaatcc ctgatccatg attatttctg catatctatc gagtaaaatg
     3721 aaatacttaa tggaagggat ttgctgtttt aatttttctg cattggctac tggctgtttt
     3781 ttcaatttga tacgaagccg agtcgcacat ggaaggacag aatcaatatt gtcttttcct
     3841 cccaactgtt caatcagcat atgaatctgc tcggacagta ccatctgttc aggattcatt
     3901 gtcgtttcca ttagaagacc actccactca ctaatataat attggcgtaa ggtgtcattt
     3961 cgcctgtacg aataaatgca tgacagttat tcaactcttt tttcatttct tcatgtggga
     4021 taaagctaat cggtgtatct ggtaacaata atttgatttt ttccaatatt tccgggttca
     4081 ttgtctttat ttcttccgcc aattcaatcg attgtacctc aagttcttct aaaacgtttt
     4141 ctaaaacaga taaaaaactt ggaagaccgt ttgagacagc caaatctatt ttttcagtac
     4201 caaaggggac aggcatgcct gcatccccga tacttaattt atcaaagtgc cccatttgtg
     4261 cgattactcg tgaaatatct gaattgatta cttttccttt tttcatgttt gtttcttcct
     4321 ctcattgtta attttagact gctgattgtt ctaaagcttc gcggtaaggg atagaaggtt
     4381 gcgcaccata agactgcaca gcaacagaag aggctagatt tccataaagg atcgcttctt
     4441 tcaagttact aaggtctggc tgaagtgtgc tgcttaatgc gccaataaat gtatcacctg
     4501 cggctgttgt gtcaacggca tccactttaa aagcagggac gataccgtgt tctttttctg
     4561 tatgatagaa agcgccggca ctgcctagtg taatgattac tgttccaata cctaattcat
     4621 ggagtttttc ggctgctgcg accaaactgt tatgatctgt cactctgatt cctgtaatga
     4681 tttctgtttc tgtttcattt ggtataatga tatctgtctt ttcaagtagt tcagtaggaa
     4741 tcgtttcacg agctggtgca ggattcagga tcgttgtttt tcctgcgtct tttgcaatag
     4801 aaaaagctag gatcgtcgca tctaagctac tttcaaactg agcaataata aagtcactgt
     4861 ttgtaatcag ttgcttgttt tttaggactt cttgttcatg gaaagcatta tttgcccctg
     4921 catggatcaa gatactattt tctcctgctg cgtctaccat aatcattgct tgtccggttg
     4981 tggcgccttg gatttcagct atcccagatg tatcgatgtt ttcttttgtc agtagatcta
     5041 ataactgttg tccttcgctg tcagccccaa cgcctccaat aaaagatgtc cgtgcgcctg
     5101 aacgtttggc tgccactgct tggttggcac ctttacctcc gccagcgtga aaaatctcat
     5161 gcgcatgcat cgtttcgcct ggcttgggca tgttggttaa acgaagggta gtgtccatat
     5221 taatacttcc aattactgta accgtgttca ttttacaaac ctccaactat ctcatttatt
     5281 ttgatgggaa aattattttt taaaacgttt tagtaaaacg ttttaatagt tcgataaaaa
     5341 tatagcacac cttgaaagct atttcaaggg tgcagttgaa aatctttttt ctaatcgtac
     5401 aggtaatctt ttttcttccc attctttttc tgggaattgg atccgatcta acaataactt
     5461 ggcagatgtt tgtcccaatt cgaagattgg ttgtgcgatc gttgtcagtt tcggcttgat
     5521 atactcgcac atatctatat tatcatagcc aataatgctg tagtcttcag ggatgctttt
     5581 tccagcttcc tctaatccgc gatataatcc aaaagctaat tcatcattta aagcaaacac
     5641 accagttgca gcagaatcaa gaagttctgc tgtgatctgg tagccacctt gttttgagaa
     5701 ctgtgtaggt aagaggatca gttggtcgtg agggatttgg taaacatcaa gagcagattt
     5761 aaacccctca atccgagcgt ggacattttc cggtggattt tcaggataga caagggcgat
     5821 cgtttgatgt cctagactaa gcaagtgcat gcccgctagg taaccaccgc gaaaatcgtc
     5881 tgttcgtacg gcatcactaa atccttcaga ttttttctga tccaaaacaa tgaagggccg
     5941 gccttgtttt ttcaaatttt cgttgattgc atctgtagag acagcagaag tcgcgataat
     6001 gaatccatcc acacctcgac gtgtgagctc ggctagatat tcgatttctt tttgatgatc
     6061 agaatcagca ttacataata tcgtgacgaa attctgctta tacaagatat cctcaattcc
     6121 tcgcatcagt gtactgaaga atggattcgt gatatcagga acgagtacac caattgtttt
     6181 cgtttccttg gtaatcattt gtctagcaaa atagtcaggc tgatagccta attcttcttt
     6241 ggcagctaaa actttctgaa cagttttcgg actgaatttc tccgtatttc catttaatat
     6301 caaggagaca gttgtgattg aaacgccgga aagtttagcg acttctttga ttgtgatctt
     6361 ttttttcacc atgttcatac tcctatctaa atcctgtggt tattttccca tgaaatcaaa
     6421 aagaagtaaa tgaaaatcac gtgtgccagt acttatttgc tttgttggat agcttcgatt
     6481 atttgtttcg ttgagcgtac tcgtgcagtt ttcggaaata tcttttttat gaacaattga
     6541 tgggattctt tgtcacgatc actgcaagca tcttcagcaa gcacgatatg gtacccgtgt
     6601 tgaaaggcat ctaatgcagt agcatataca ccgtttgttg tcgctacacc gcttaaaatg
     6661 atagtttcga ttccacgccg gcgaagttga agatcaagag aagtaccaaa aaaagcactt
     6721 ggattgtatt ttgtgatagt caacatatta tctgtatctt tcaaagaatc cttcaaaagt
     6781 aaatcagtga attctggagg tacagaagca gtcattccac ccatatcagt ttgttgactc
     6841 aaataaggga aagtggagat gtctacgttc actaaaactt tcaaggcagc ggtacgtttc
     6901 aatgttctta ctaacaaatc attgtttgtc agaatagatg ctgcagtgtg tggagagagt
     6961 gtaccgttat ttacgatctc tttttgcatg tcgatcgcga caaaagcagt ttttgataaa
     7021 tcaaaaatca tattttcctt taaatcattc atgatgttcc ttctttcaaa taacaatgtt
     7081 ttttatgtaa gtaaatcata aatcgtttat ttgtcaacta aattaaagga ttcctttctt
     7141 tagtctagag aaattcagtg gtaaaataaa taagtgtgag aaaacgcatt cgatgcatag
     7201 atatagcgag aaatatcgtg aaaatggagg aatcagagtg aagtatgtcg aagtaaaaaa
     7261 tgaatacaaa cgatacaaaa tgggagaaac gaccattaca gcaaatgacg atatttcttt
     7321 ttcaattgaa aaaggcgaat tagtgattat acttggtcca agtggagcag gaaagtcaac
     7381 gattcttaat attttaggtg ggatggatac acctgatgaa ggtcagatca ttattgatga
     7441 cacagatatt gcgcaatttt cagataaaca gcttactgct taccgacgaa cagatgtagg
     7501 gttcgttttc caattctaca atctcgttcc taatctaaca gcaaaagaaa atgtggaatt
     7561 ggcaacggaa gtttctcctg atgcgcttga tcctgtagag gtattgaggc aagtgggcct
     7621 agctcatcga ttgaataact ttccgtccca attatcagga ggcgaacagc agagggtttc
     7681 aattgctcgt gcactagcta aaaatcccaa acttctttta tgtgatgaac cgacaggcgc
     7741 gctggatttt gaaacgggca agcaagtatt gaaattattg caaaacgcta gcagacaaca
     7801 cggcaatacc gttttgatca ttacacataa ttctgcttta gcgcccatcg ctgatagagt
     7861 cattcacatt aatgatgcta aagtaaggtc tgttgaatta aatgatcatc cttcatccat
     7921 tgatgagatt gtatggtagg gggattttat gaaaacaaga acatatctta aagcaggttt
     7981 tcgcgaaatt cggcaatcta aaggacgatt cattgcaatc atccttattg tcatgctagg
     8041 aacactttta tttgtaggtg tcaaaactgc tggacctgtc atgcaaaaaa cgatggatca
     8101 ctatgttggt acagcaggac tttctgattt gcaaatcgtt tctactggtg gcttgacaca
     8161 aaaagatatt gctcaagcag aaaagatccc agatgcaaaa gtagaaacag gaaaacagat
     8221 ctattattcc aatactggca aaaacgaagt gattcaaata ttttcttata acaaaaataa
     8281 caagcagaac aagctacaat taactgatgg aaaactcccg gaaaaaccaa accagcttgt
     8341 attggatgaa aaagcggaag atgaagggta taaaatcggt gatatttatc agattgattc
     8401 agatgaattg aaagaaaaag agtataccat cacgggcttt gtccgttctc cattgttcat
     8461 caacaatttg gagagaggat acgcgaacgt cggaaatggt agcgtcgatt attttgtcta
     8521 tttacctgaa tccgttttta aaacagatat ttattctgtc ttatatcttg attttacaaa
     8581 cgtaaagaat ttgaatacgt acagcaaagc ttacaaagat aaaatggagc aaaatcaaga
     8641 aaaggcagaa aagtatttac aagatcgacc cgaagaacgc ttagaagagc tgaaaaaatc
     8701 ggccaacgaa gaattagaac cggcacagca aaaaatagcg gatggcaaag cacaaacaac
     8761 gcaagcaaga acacggttag aagatgctaa aaaacagtta gcgcaacagt cagcaactat
     8821 ccagcagctt cctgaagcgc aacgaacaat cgctgttcaa acattatctc aacaagaagc
     8881 tcaacttgct gaacaagaaa ggcaattagt tgagaaagaa cgtgaactgg cctctgcaga
     8941 ggaagaattg aatcgaaatg cagaaaaaat tgaaaattta actaaaccat catacctata
     9001 caatgaacgt tcagaaaatg tcggttttca agaatttggt gatctgtctg atcgtatcgc
     9061 tgcgattgcc aatgtttttc ctgttttctt tttctttatt gccgcattga tcacctttac
     9121 gacaatgaca cgtatggtag aagaaaaccg cagggaaatc gggacattaa aggcgttagg
     9181 ttatacgaaa gttgaaatcg caaaaaaata tgcaatctat gcaagtctag cctcagggat
     9241 aggtattgtt ttaggaacga tattaggaac gaatcttttg ccaaggatca tttttgagct
     9301 gtctaacgag cggtatgata ttgggagtgc gcttattttt tatgactggc cgccgatttt
     9361 acaagcagct gccgcattct tcattgccgc atttggcgct gcaatgatcg ttttattcaa
     9421 agatttaaga gaacggcctg ctgcattgct tcaaccgaaa gcaccgaagc cagggaaacg
     9481 catttttctt gaatacatca ctccgctgtg gtcacgcctt agctttaatc aaaaaatcag
     9541 ttaccgaaat ctcttccgat ataagtcaag aatgcttatg gcgattatcg gaattgctgg
     9601 atgtgccggg ctgatggttg caggcgtggg attgaaagat tctctaagtt ctgtttcagc
     9661 caaacaattt ggaccgatca ttgactatca agcaatcgtt acattggatt catccacgga
     9721 agatagtcgt caaaaagcag cggatatgct gcagaagcaa gaaaaagtaa atgagatcct
     9781 agctattcat actcaaaccg tagaattgag aaaaaaagga catgcttcgc aaagtctgac
     9841 agtgatcgta ccaagtaaaa cagctgattt ggatcgtttt atccatttaa ctgatcttga
     9901 agagaaccca cgctccttgc ctaaaacagg aatcgctatt acgcaaaaag tagcagaact
     9961 atttgactta tctacaggag atcatatttc gctttatgat gaagatcaaa aagaatggaa
    10021 agtcgaagta aaagaaatcg tgaaaaacta tctaggtagc tttatttata tgtcccctga
    10081 atattatgct tcagttactg atcaatcgat gatggataat gcatttttgc tgcgtacaga
    10141 taagatgaca acaaaagaag aagaaatgct ttccgagaaa ctgcttgcca cagacgctgt
    10201 gacaaatacg tctttcatgt ccaaacaaat cgaaacgcaa gaagaatcaa tggcgaactt
    10261 agactcggtt gtcttaatct ttgtcgtatt atcaggactg ctggctttta tcgtcttgta
    10321 taatctaact aatatcaata tttccgagcg tgtacgcgaa ttatctacaa taaaagtcct
    10381 tggatttttt gataaagaag tcacgatgta cattgttcga gaaaatatca tctttactct
    10441 tttaggaatc atcggaggat ttggtgtagg atatatattg actgacttca tcttgcaaca
    10501 agcgtctatg gagaatgtca tctttccatt agtgatcaca tggtgggctt atgtcttatc
    10561 agcaggatta actatcgtat ttacggttat tgtgatgatt gttacccatt tcaaattaaa
    10621 gcatatcgat atgattgatg cgttgaaatc gaatgaataa aaacagaggt tgtgaaatca
    10681 gcggtttgac ctgagtatta gagaaacaaa cggcaaaata gtttttcata ttttgccgtt
    10741 ttttgagcta ataccgaagg acagctgatt gaacaccatg aatcagaggt tgtaaaaaag
    10801 ccgtcctgca tcgagcaaaa agaatactca aataaaatta gctcctcatg ttttacattt
    10861 gagaagactg attgccgtag ataaaagctt ttgaacacca tttatagaag aaacaagaat
    10921 attttgctat tttaaacaag aggatagaca aatacatgtc tgatctcttg ttttttattg
    10981 ggcaacagac ctcttagaat gtataaagat aaattttaca aacgctttcg gttcagttat
    11041 aataataaat gataacttgt atatataagt tgtggtaggt aataaatcag ttgaatggag
    11101 gtaaatatcc atgacattta aagacaaagt catttaccaa atctatccca aatcttacaa
    11161 agacagtaat ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac tcccgtatct
    11221 gaatgatcta ggcatcgaca tgatttggct aaaccctatt tatccaagcc ctcaaaaaga
    11281 caacggttat gatatctctg attacacagc catcgatcct atctttggta cgatggagga
    11341 attcgaacag ctaatagccg aagcaaagaa ataccggata gaaatcatgc tcgatatggt
    11401 gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa gcactagcag gggaccagca
    11461 ttatcaggac ttcttcattt taagagaaga gccaacggat tgggtatcaa aatttggtgg
    11521 aagtgcttgg tcgccatttg gagatacggg gaaatattat ttgcacttat atgatcggac
    11581 acaagctgac ttgaactggc ggaaccaagc agtaagagag gaactattca aagttgttcg
    11641 tttttggatg gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa
    11701 agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata cggacaaacc
    11761 aatcacccat acgtatctaa aagaattgaa ccaagcaacg tttgggacgg attccactat
    11821 catcacagtc ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt atacagaacc
    11881 tagtagagaa gaattatcaa tggtctttaa tttccatcat ttgaaagtcg attatgaaaa
    11941 tgggaataaa tggtctactc ctccatttga ttttgcagaa ttaaagcgac tatttcatac
    12001 atggggagaa aagatgagtg agggtaacgg ctggaatgct ctatttttta ataatcatga
    12061 tcagccacgt gcgctcaatc gatttgtagc tatcgatcag tatcgaaaac aaggagcaga
    12121 gatgctagca accatgatcc atttgaaccg tgggacgccc tatatttata tgggagaaga
    12181 aatcggcatg atcgacccag actattcatc gatttccgac tatattgacg tagaaagttt
    12241 gaatgcttat aagttactcc ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat
    12301 caaagcaaaa tctcgcgata actctcgaac acccatgcaa tggtcagaca cagaacaagc
    12361 aggatttact acagggaaac cttggttaaa agttggaggc aagctagaag agattaatgt
    12421 agaaaaagaa ctatcttctg aagattctat tttgtcctat tacaagaaat tgattcggct
    12481 gagaaaaaca tatccaatcg ttgcacaagg agattatcat gcttatgaag caaatcatcc
    12541 ggaagtttat ggctttcttc gccaatttga aggacagcag ctgcttgtat taacaaattt
    12601 ctatgataaa gatacaagga tcgccatccc agaaggattc acagaagcca atattcttat
    12661 cgataattat tccgatactg ttcttcatca aagtctggag ctaaaaccat accaggcaat
    12721 tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc tattctcgta
    12781 agatcgacgt tttgtgtttt atttaaaata atcttcttca gaaaatgaag agttttcata
    12841 ctctccttta tcgccataaa atttattgta gcgttgtttg aatttacgga aaataaaagt
    12901 aacgagtaat ttgactaacg tatagatagg aataccaaaa ataacgccca caattcccat
    12961 tagatcaccc atgacaagta aaacgattaa gatcgtaatc ggatggatct gtaaacgatc
    13021 acccattact cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa accagacaat
    13081 gacaaatttg acagccataa aagtagagtc ttggaatgca atgatcaaac ctggaaagaa
    13141 ggcgataaac ggcccaatat atggaatgat acataacaac ccagcagaaa tagctaaaac
    13201 actagcaaat tctaatccga tcagtaagaa acaaacccag tagactgctc caaggataaa
    13261 tgaagcaatg atttgcccct ttagaaatga acccaattgt tgattagcaa tctttgtcaa
    13321 atttttaaaa tctgtacgaa atgctggtgg gacaatcgct aaaactgact gataaaattt
    13381 ttgaggatct ctcaaaagga aaaaagcgac aattggacca gtaaataaag taataaaaat
    13441 agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc aataatttcc
    13501 aataaatcca gctatatcag acgtcacgtc gtcaatagaa gcgaaaaatt gattaaatga
    13561 atcggcaaaa ggtgttttct ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa
    13621 atctggcaaa ctactgatca atgtctttgc ctgttcttca agaaaaggaa taagccatat
    13681 gcttccaagc cccaataaaa ataaaataat cacataaacg ataatgactg acaagttcct
    13741 cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa agataataca agatcagtgc
    13801 aaacaataat gggggcaaga tagtagcgat aatgattccg acaggcttga atataaacga
    13861 gatctgatgg aaaaaataaa tgatcagcgc aagtaagata actattccta aaacatagta
    13921 agaggttttt cctcctaaaa agcggatcaa acgattcgtt gtatctgttt cttttttcac
    13981 tctttttcct cctttatcta taacttcatt atagacaaaa gcgggtataa aaggaaaatg
    14041 atagaggttg attgcaaaaa tatcgtcaat cattcatata aactttctca tcaattaaaa
    14101 taagaaaacg cttgacagtt cttttaacca tatgataaat taaatcctgt tgctaaaacg
    14161 taactattac ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag atgagaagag
    14221 gagtgtcata aatgaaacga ttgataggta tattggccat gctggtttta gcaggtatgt
    14281 taactgcttg cggggcaagt gggaaagccg aggacagtaa agaaaaattg agcgtgatga
    14341 cgacctttta tcccatgtat gattttacaa aagcaatcgt cggggatgaa ggagaagtag
    14401 agttattgat tccagcaggg actgattccc acgattatga accttctgct aaagacatgg
    14461 caaagatcca agatacagat atctttgttt ataatgatga aaacatggaa acatgggtcc
    14521 ccgcaatcca aaaaacatta caagaaggca atgtccatac gataaaagcg acagaaggta
    14581 tgcttttact tccgggaagt gaagaaggac atgatcatga ccatgaacac ggggaagaag
    14641 gccatacaca tgagctagac ccacatgtat ggctagctcc aagtttagca atcaagcagg
    14701 tagcaaacat acgtgatcag ctgattgaag cttacccaga aaaacaggaa gtatggacaa
    14761 aaaatgcagc agcctatacc gagaaacttc aagcactcca tcagctttat caagaaacct
    14821 ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac tatctagcgt
    14881 tagaatatgg attgaatcaa gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg
    14941 cggcgagaat tgctgaactg aaacactttg ttaaagaaca tggaatcaac tatatttatt
    15001 ttgaagaaaa cgccaaagat agtattgcca gaacgttagc aaacgaagca ggtgtatctt
    15061 tagaagtatt gaatcctttg gaaggattga cgaatgaaca aatagaaaat ggagaaaact
    15121 atctttctat catggaagca aacctcgaag cgttgaaaaa aacgacagag acagaaaacc
    15181 cgctagaaga tactttgacc cccaaaaaag aaaagagcgt ctacaacggt tattttgaag
    15241 acgaggatgt acaagatcgc tctctttcag attgggcagg aacttggcaa acagtagata
    15301 cgtatgtaga agatgggact tttgatcctg tttttgaata taaagaaaaa ttgaatcagg
    15361 ataagacagc acaagaatat aaagaatatt atacgaaggg ctatcaaaca gatattgctt
    15421 caatcaagat cagtgagaaa gagatggcat ttacgtttaa gaatggaaca acgaaacgtt
    15481 caaattatcg ttatgtaggg aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa
    15541 ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt tagatatatt caattcagtg
    15601 atcacacgat tgcaccggct gactccgaac attttcattt atatgcagga aatgaaagtc
    15661 aagaagtatt gctgaaagaa atggagaatt ggccaacatt ttatccagat acaatggatg
    15721 gacgtgaaat tgcacaggaa atgcttttgc actgataggc aaaaaagaac cgaagggttt
    15781 ggatcggctg gtcatttagt cggtccaaac tattcggttc tttttatgaa atcaatttct
    15841 ttaatccacg gatagaggct gggatggtct cgccgtttgc caacgtgact tctctggttt
    15901 ttttattaat cgaccgcaca ttttgtgtat tgataacata ggatttatga caacggataa
    15961 aattccgatg cattttctcg atttctttga tcttctcata gaattgtaat tgggcatgag
    16021 tcaaatggac ttccagttta tgtggcgctc ctagcgaagt agaaaaatat tggatgtctt
    16081 tgatcggtaa aaatttgatc tcatcattaa actctatttt gaagatatct ttttggattt
    16141 gggacatttg aaaatcttcg aaaattttgc tcaggatatg ctcgacattt tcttttaaat
    16201 cataaagatc ttctttgacg atataatcag tcggttcgat gttgcttttt aagattttca
    16261 tcgacatttc cgtatgggaa gtcacataga ttatttttcc taaaggatct aattcacgga
    16321 tcaatttacc gatcgctaca ccatccattt cactgtcttc tagttcgata tctaaaaagt
    16381 aaataccaaa agaagaagtt ctgatcttgg ctgcattgag aagttcttcc ggatcagagg
    16441 tagaaatatg gacataactg tctaagtttt caatcatgat tctatttttg atcaccgttg
    16501 ataaaacttc taattgtttt ggatcgtctt cacaaatata aatgggaatc atatcattac
    16561 tcctttattt tatttgtata taaatcgttt gagaaaaacg ctggttttca attgatgttt
    16621 caaggaaaag ataaggattg gtctttgtca attcatccaa gataaacaag ccaaggcctt
    16681 cgttttttgg atttgttttt gttgagaacc ctttttgttt caactggttt aacggtaatg
    16741 tatttgttgt tgtattgatc acacgaatca tcacggaatg attcttcttg agtatggtca
    16801 attcgatttt tttcttcttt gcagcactag ctccttcaat cgcattgtcc aagataatac
    16861 caagcatacg tacaaggttt accgtatgtt tcgtatccag ttcgattggc tcaggaaaat
    16921 gaagctggac gatcaaccct ttttcctgtg ccatcatgag ttttaatgac agtaaactac
    16981 ggatctcagg aactttcata ttatccaaca tacttagatt ggccggaaaa aggttcatca
    17041 tctggcgtgt aggtaatatc gtttgatcat aataagaccg taatccttct aagtcgcctt
    17101 ctgaaatata gctgtgaaga gaaaaaagca gattttggta atcatgtttg aattctctca
    17161 agtcttgata gtttttttct agatctttga tataatcctg ttgcatcctg acttgttgct
    17221 ctaataactg atcagctaat ttcttattag agattttcca ataataaacc aagatgatca
    17281 ggccaacaca cagatataga atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt
    17341 caccagaaaa acgggtataa aaaataatga tatagtatgt aaataatatc aatgtcccga
    17401 tcgtaccgat catacggcgg tccacagtgt gaaaccaagt ctctaacgat ttttttaaac
    17461 aaaaagctaa taaagcagat aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat
    17521 ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc gccgatgctt gttaagtgat
    17581 ctcctaaaag cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag
    17641 tcgaaaagaa gaaaaaagta agcatcagca ttcctatagc aattagagca gtaaaaatcc
    17701 ctaaaatcga atagacccat ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca
    17761 gatatttgta tcggatgtga tggtcaatca ataagataca aattgtgtaa atcaaatatt
    17821 gcaacgctat ggaccataaa aacataaagt acagctccca ttgattttta ccaaattata
    17881 gcacaattgg cgtattattt ttttaatttt tcagggattt tgaattcaga actcatacca
    17941 aagcagcagc cagatacaaa aaagtcagat aagttagtta attgtttaat gattgttttt
    18001 ttcatgaaag attcctccaa aattcatcaa gtagtttgta ataggaagca acgtagctat
    18061 ttctaaaaca atcccaagta gcaaataggt agaaaagtaa gaagctgaga aaaataacgt
    18121 aatgagagaa aaaattagaa acatgctgat cgccttcttt aataatgggg gacgtttctc
    18181 gggttcaatg ggattgattg ctgtacctct tgatccatag cgaagcagta acaaaaagca
    18241 gctgattaac aaaaagacca tcattgggaa ggagatagtc aattgcagat gtttcatcac
    18301 caagggaacg ccccataaat aaagtagtgt ccacagcaga caaacgaatt cagagtcgga
    18361 atgcaatcca taagcatatc tgcggatgca gatataagaa agaaaggtga ttcctgtttc
    18421 cgcccatgag cctgtcacca gagaaaagac tagaacgacc gctagttttc caagattatt
    18481 caataaaact tctaagacaa attttacttg gacatacacc atatcttctt ccgatttgtc
    18541 tccgctgaat aaggcttcta ataatttgtt cacaacttta tctttcatct catacgtacc
    18601 tccaccacaa attttatgct aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt
    18661 tttgacatca aaaccgtcat gatctcctaa aaaaatcttc gttttccaag aaccttacca
    18721 aaaaaagtga cagttttgat acgaaaagta ctttttatga caagtttgtg ctttttcgag
    18781 tgatttatgt taaaaaatta ctgaccttat aaaaggtgat ggagggattc ttatggaaaa
    18841 tgaacaattg tttgaagtcg gcgaaacata tgcatttaaa tctgatttat ttgaatcacc
    18901 aataaaagga aaaatagaaa aaatctatga aaatagtgtg atgatacgtg tcatgaaatg
    18961 cgcagtagaa gacaatgata ccgctcataa tctgcactgg cagttagttg tacgcaaatc
    19021 aagtacaata gaagaataaa aacagataaa atgttgagag cataaagagg ttgtgagtct
    19081 cccgtttagc tctgagttac aaggaataat gttaaaaaac agcttttcat gttttatgaa
    19141 tattgccttg tgaccgaggt gttggcagga gaatactgtt tatttgaaaa aagggctgcg
    19201 acaagacttt tgtcacagcc tttattttca tgaataggag ttacttttgg ctacttttag
    19261 agaagtagct tttaaggagc agataaagtc aaggctttta tttctataaa tgaagtagta
    19321 aaatgaagaa cagattttat tacaggaagg atgccgataa ttgtgatcat tgttgcagga
    19381 acgatcggtg cagggaaaag cacactaact gaaatgcttg cccaagattt agaaacaaaa
    19441 cctttttatg agaacgtgga agataatgaa gtattgccgc tattttattc taatccagaa
    19501 aagtatactt ttttattaca gattttcttt ttgaacaaac gctttttggc aatcaaagat
    19561 gctttcagcc atgatgacaa cgtactagac cgctccatct acgaagatag tatgctgttt
    19621 catttgaatg cagatttagg gcgtgtttct gaagtagaag taaagcagta tgaaggtctt
    19681 ttagaaacga tgctgaagga gttagaagaa atttcgccac aaaagaaacc agatctactg
    19741 gtttatattc gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa
    19801 tatgaacagc tagaacaaga tcctgaactc tattcctact ataaagaact caatcgacga
    19861 tacgaagagt ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga tggggacaaa
    19921 tatgatttcg tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg
    19981 gcaaagaaaa tgacagaaga ggcagatgcc cagtcgatcc gctacactac aaataacccg
    20041 aaagcaaagg gaatctttac aactaaataa tataaaaact aagcaaattg ctcactcgca
    20101 gatatttctc tgtgagcagc atcttgctta gtttttttga ttcattgaga tcaaatagtg
    20161 aaccgattcg tatgataaaa ttgccataaa tgaaatgtct gagtgagggc catgatcaaa
    20221 aataaaatgg aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg
    20281 acaatagctc ctgcgataaa ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa
    20341 tctgcttttt ctttagaaat acaataatca tataagttcg ttgctacccc ttttaaattt
    20401 ccagttgtaa ataaattagt gtaggcacgc ccgttgattt tatcaaaagc tacccactgg
    20461 atcgatgcag taaaagcaat caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc
    20521 aaactaacaa agaaaaaaat cactgcttca taatatagaa caaacaagcg ccagaaatgt
    20581 ttttcttttt ttctaaaata atcaattaga tactttgtta gaagaatccc taagaaaaag
    20641 aaaagggtag acaatacttt tttaccaaca cttagccatt ctccgtcaaa tgcttggatg
    20701 atcgatagaa tgatattgcc agtttgagca gaggcaaaag caccatattg gatataagta
    20761 taactgtcca tggctcctcc gataaatgtc agcaaaagtc ctattgtacg cccctcgtga
    20821 aaggaagtct gcattttttt catcaccttt tatgaatatt tttgtcttcc ttatcttagc
    20881 ataaacgatt gaaaaaaaag gaaaaatttt cagaaaacta aaatcatatc gcttcccatt
    20941 ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac tttaaataac
    21001 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
    21061 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
    21121 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
    21181 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
    21241 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
    21301 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
    21361 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
    21421 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
    21481 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
    21541 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
    21601 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
    21661 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
    21721 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
    21781 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
    21841 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
    21901 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
    21961 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
    22021 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
    22081 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
    22141 aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
    22201 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
    22261 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
    22321 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
    22381 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
    22441 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
    22501 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
    22561 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
    22621 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcgaa
    22681 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
    22741 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
    22801 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
    22861 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
    22921 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
    22981 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
    23041 aaagaaagta atcgacttta atgaaataaa agcagaacca gtaacgacga tagaagaaga
    23101 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
    23161 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
    23221 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
    23281 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
    23341 gaagctattt ctggaacaca gtttattcgg aaaagggagg tgtgacaaga at
//
LOCUS       DACCWV010000032        23359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCWV010000032 DACCWV010000000
VERSION     DACCWV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.032"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            139..996
                     /locus_tag="IVW84_001096"
     CDS             139..996
                     /locus_tag="IVW84_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7917305.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1111..2031
                     /gene="pstC"
                     /locus_tag="IVW84_001097"
     CDS             1111..2031
                     /gene="pstC"
                     /locus_tag="IVW84_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7917306.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2031..2915
                     /gene="pstA"
                     /locus_tag="IVW84_001098"
     CDS             2031..2915
                     /gene="pstA"
                     /locus_tag="IVW84_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7917307.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2930..3736
                     /gene="pstB"
                     /locus_tag="IVW84_001099"
     CDS             2930..3736
                     /gene="pstB"
                     /locus_tag="IVW84_001099"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7917308.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3752..4510
                     /locus_tag="IVW84_001100"
     CDS             3752..4510
                     /locus_tag="IVW84_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7917309.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4523..5200
                     /gene="phoU"
                     /locus_tag="IVW84_001101"
     CDS             4523..5200
                     /gene="phoU"
                     /locus_tag="IVW84_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7917310.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5438..5737
                     /locus_tag="IVW84_001102"
     CDS             5438..5737
                     /locus_tag="IVW84_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917311.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5763..6470
                     /locus_tag="IVW84_001103"
     CDS             5763..6470
                     /locus_tag="IVW84_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7917312.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6467..7234
                     /locus_tag="IVW84_001104"
     CDS             6467..7234
                     /locus_tag="IVW84_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7917313.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7334..8902
                     /gene="liaX"
                     /locus_tag="IVW84_001105"
     CDS             7334..8902
                     /gene="liaX"
                     /locus_tag="IVW84_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7917314.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9202..9519
                     /locus_tag="IVW84_001106"
     CDS             9202..9519
                     /locus_tag="IVW84_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7917315.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9519..9869
                     /locus_tag="IVW84_001107"
     CDS             9519..9869
                     /locus_tag="IVW84_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7917316.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10036..10974
                     /locus_tag="IVW84_001108"
     CDS             10036..10974
                     /locus_tag="IVW84_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7917317.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10989..11819
                     /locus_tag="IVW84_001109"
     CDS             10989..11819
                     /locus_tag="IVW84_001109"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7917318.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11863..12888
                     /locus_tag="IVW84_001110"
     CDS             11863..12888
                     /locus_tag="IVW84_001110"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7917319.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12903..13841
                     /gene="galU"
                     /locus_tag="IVW84_001111"
     CDS             12903..13841
                     /gene="galU"
                     /locus_tag="IVW84_001111"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7917320.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13966..14406
                     /locus_tag="IVW84_001112"
     CDS             13966..14406
                     /locus_tag="IVW84_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7917321.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14409..15011
                     /locus_tag="IVW84_001113"
     CDS             14409..15011
                     /locus_tag="IVW84_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7917322.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15079..16185)
                     /locus_tag="IVW84_001114"
     CDS             complement(15079..16185)
                     /locus_tag="IVW84_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7917323.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16395..17396
                     /gene="ccpA"
                     /locus_tag="IVW84_001115"
     CDS             16395..17396
                     /gene="ccpA"
                     /locus_tag="IVW84_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7917324.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17732..20077)
                     /locus_tag="IVW84_001116"
     CDS             complement(17732..20077)
                     /locus_tag="IVW84_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7917325.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20356..21258
                     /locus_tag="IVW84_001117"
     CDS             20356..21258
                     /locus_tag="IVW84_001117"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7917326.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21471..23246
                     /locus_tag="IVW84_001118"
     CDS             21471..23246
                     /locus_tag="IVW84_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7917327.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7444 a         4142 c         4926 g         6847 t
ORIGIN      
        1 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
       61 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
      121 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
      181 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      241 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      301 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      361 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      421 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      481 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      541 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      601 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      661 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      721 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      781 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      841 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      901 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      961 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
     1021 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
     1081 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1141 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1201 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1261 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1321 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1381 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1441 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1501 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1561 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1621 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1681 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1741 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1801 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1861 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1921 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1981 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     2041 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     2101 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2161 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2221 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2281 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2341 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2401 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2461 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2521 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2581 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2641 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2701 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2761 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2821 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2881 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2941 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     3001 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     3061 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3121 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3181 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3241 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3301 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3361 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3421 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3481 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3541 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3601 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3661 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3721 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3781 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3841 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3901 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3961 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     4021 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     4081 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4141 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4201 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4261 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4321 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4381 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4441 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4501 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4561 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4621 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4681 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4741 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4801 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4861 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4921 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4981 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     5041 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     5101 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5161 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5221 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5281 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5341 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5401 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5461 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ctgctttata
     5521 tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa gaggatcatc aattgattac
     5581 aggaagactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5641 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5701 tggagatgaa tggcagctag tagcaaaaaa aaaatgatgg ctggcttttc ttcttcacaa
     5761 gtatctccat tggcgccatg ttgcttattg gcggaaaaca gggtctattt gctttaggaa
     5821 gtctcatcat caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag
     5881 ggatttctct attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat
     5941 tggctttaga tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg
     6001 tttttttagc attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc
     6061 gctatgagga actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac
     6121 tgattggaac agctggagga acactcgata ccgttatcac agtcattgcg acattagaag
     6181 aattgactaa gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag
     6241 tgggtcgaga tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt
     6301 cagttccatc gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac
     6361 agcatttatc attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga
     6421 tccctatctc ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat
     6481 tatacttgtt gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta
     6541 tattctttta ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca
     6601 tcagaagtgg ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat
     6661 cttatttttt atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat
     6721 ctttttattt tgtttcctac tattgcttcc tatcactgat tttttagcga tccaaggttt
     6781 tacatcggta gaactagaag aattatctgg cttagataag actttagcga ttgattttag
     6841 attacttacg agatcgttgc ttctgattag tcttagtggt gctgttttag atgctagtgt
     6901 agcaatttct agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca
     6961 attgcgccat tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact
     7021 tctttttgca ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat
     7081 tccttttcag caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct
     7141 taccacattg tctgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt
     7201 tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat
     7261 ttttactgaa tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa
     7321 ggaggcaatt tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct
     7381 ttcgactgaa gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca
     7441 aataaaaaaa gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca
     7501 attgatcgat aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga
     7561 aagagaaagg caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc
     7621 gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc
     7681 acgcaatgag aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt
     7741 gacagaagaa gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc
     7801 tagtggcgat acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg
     7861 taagaatcaa tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga
     7921 ttggaaagat caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg
     7981 atcacagtta ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga
     8041 caacatggaa tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga
     8101 gcatgaattt tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg
     8161 aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact
     8221 ttacggaaaa atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt
     8281 taatgaagat catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt
     8341 ctacttacct aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat
     8401 gattgaaaca ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt
     8461 ggaccaatta actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg
     8521 aaacgggaac atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc
     8581 gacagcagaa aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga
     8641 agataatttg aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc
     8701 agatggcatc ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct
     8761 gtccaattac gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg
     8821 tcgtgtttca gatggtgaaa tcacgcaagt gcaactttca acgacaacag gaagtatcta
     8881 tttaaaagat acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag
     8941 aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgataggc
     9001 tatctcttga acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa
     9061 ggaataattt cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat
     9121 tttgcagaaa gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata
     9181 gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt
     9241 ttgacaggga ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt
     9301 gtgctttatg tattcctgag cttagttgct tttggaagtc caatcatttt atacatttta
     9361 ttagctttga ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca
     9421 tattatgaaa aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa
     9481 gcggaaaaaa tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt
     9541 tagttaatac gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga
     9601 gcagtatttg gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca
     9661 aaccgatttt gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt
     9721 ttgttatcaa tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta
     9781 gtttttcttc attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa
     9841 ttgtcagtga acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt
     9901 tttttattta gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt
     9961 ttttaattgg tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga
    10021 aatgaggtag catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct
    10081 ttggaaatcg tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc
    10141 tcacgcccag gactagagtt gactggttat tttaattatt attcccataa tcgtttacag
    10201 ctatttggca gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc
    10261 atcatgagaa gattatgtag tgaagatatg cccgcattta ttatttcaag aggattaccg
    10321 gctccagaag aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc
    10381 atctcaactt ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct
    10441 cgaacaagtg tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt
    10501 gacagtggga taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg
    10561 atcgcagacg atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg
    10621 cctaaaatcc tggaacatct aattgaaatc cgaggaatcg ggatcattga tgtaatgaat
    10681 ctttttggtg cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa
    10741 gcatgggcaa aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc
    10801 gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc
    10861 atcattgaag tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa
    10921 acatttgaag aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga
    10981 gtgacaatat gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct
    11041 actggtatgc attgatcatc gtatcaggta tcattatcgc catgtggctt agcagcagag
    11101 aggctgttcg ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc
    11161 ctatctccat tattggtgct cgcttatatt atgttttatt tgatcttccc cagtatatag
    11221 ctgatccaat ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga
    11281 ttgcaggcgc gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt
    11341 ttttagatat cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt
    11401 tcatgaacca cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt
    11461 taccgcaatt catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc
    11521 tttatgaatc actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac
    11581 cacacctttt gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc
    11641 gtttctttat tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt
    11701 ctcaattatt atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa
    11761 aaaaaggaaa tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat
    11821 ttttagagcg ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg
    11881 gttttaggac caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat
    11941 gacgttcgga tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg
    12001 aatcaacgtt atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg
    12061 gaagatgcag taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt
    12121 tctgttgcaa aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt
    12181 aaaggattag aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca
    12241 gcagaaaaaa gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagcg
    12301 aaacatgaca tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta
    12361 caagagctgt tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta
    12421 gagatggggg cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta
    12481 gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt
    12541 ttaggtgtag caatgggtgc caacccgctg acctttatcg gattaagcgg agtaggcgat
    12601 ctagtcgtta cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga
    12661 caagggcagc ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat
    12721 actactcaag cagcgatgga actagcccaa ataatggaaa tcgaaatgcc catcactcaa
    12781 acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg
    12841 ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt
    12901 atatgaaagt caaaaaagca gttattccag cggcaggatt aggaacaaga tttttaccag
    12961 caacaaaagc tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca
    13021 tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt gatcgttaca ggaaaagcta
    13081 aacgaccaat cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa
    13141 aaggaaagac cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca
    13201 tccgtcaatc tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg
    13261 gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat ggaagatgaa gtaccattga
    13321 cacagcaatt aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag
    13381 ttcctcatga tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg
    13441 ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt
    13501 tagctattat tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac
    13561 aaccaggagc cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac
    13621 aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt
    13681 ttatgaaaac aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg
    13741 agtatatcct gtctttaggt aaaaaattag aagcagaagg aagcaagatc gatcataatc
    13801 caaaaggaat aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct
    13861 tcagctgcta attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga
    13921 aaatgctatc cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa
    13981 attgcaggat tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg
    14041 ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg
    14101 acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttgctg
    14161 gtcaaaagta atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg
    14221 ttcacagcag ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac
    14281 atcgcgacga aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa
    14341 gtcggtggga cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga
    14401 aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggaacagccg
    14461 cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag
    14521 ctgcccaagc ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa
    14581 aatcgactga gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag
    14641 gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg
    14701 aatctgacga tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca
    14761 aaaaaacggc agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata
    14821 cttcagaaga tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca
    14881 atgaaggaat gtcagaagca aaaccagttg cagatgacac aaaagaaatg atgaaaatgg
    14941 caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta
    15001 caaatcacta aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag
    15061 ctctcacctt ttttactctt atttttctaa aactagtaat tcttttggtg tagtcgtgaa
    15121 tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc
    15181 aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt
    15241 tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg
    15301 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    15361 acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat
    15421 gttgtatatt ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc
    15481 actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat
    15541 cttcgtgttt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    15601 gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcgattt ctgcaatgat
    15661 cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc
    15721 ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg
    15781 ttggatgatg gaagctagat cagtgtaaga atccgttccc gtcaagatat cagagatagc
    15841 atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata
    15901 gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg
    15961 gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag
    16021 gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc
    16081 gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg
    16141 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    16201 ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa
    16261 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga
    16321 ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    16381 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    16441 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    16501 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc
    16561 gtggcttggc aagtaagaaa acaacaacag ttggggtaat catccctgat gtcagcaata
    16621 tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata
    16681 ttatcttagc aaattcagat ggtaacgacc aaaaagaagt caatgtattg aataacttac
    16741 tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg
    16801 gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac
    16861 aagtaggtag cgtaaacatt gattatgtat cagcaacaaa agatgcagta aacaaattgg
    16921 caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cctatcaatg
    16981 gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg
    17041 aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga
    17101 tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt
    17161 tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta
    17221 acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat
    17281 acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    17341 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa
    17401 aggacaacca ttcagaggtt gtgagcctgc cgtttaactc tgcgttacaa ggagcaattt
    17461 caaaaaacag catttcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag
    17521 aacaccgttt attcgggaaa aggggttatg acaaaatttt gtttaatcct ttttttttgt
    17581 cttaaaaaag agagaaagtg gttgaaatta catttatcgt atataaaaca atccgtgtat
    17641 agatgctgca ttatgttcac caccatcaaa aaaataaggg actgtgacaa tactattgtc
    17701 acagtccctt attactgtgt attctattaa atcaatcatc tttctcatct gattctttgg
    17761 ttgcttgatt cgctcttgca taagctgaag cggtgttcca gtaatctgta tagttttcat
    17821 cggtaccgcc aacgccaaat ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg
    17881 attcaactgt ttttcctggt gtttggatgg attttttatt gacagtaact tttccaggtg
    17941 tttgaccagt aaatccagat acttttttct tcatcacatc agatgataat tcgaatttct
    18001 catcagtgcc aaaaatgtca ggttttgctt ggtaagcttg attgatcaga ttggcaagat
    18061 aattagccga acgtatcctt gctttgctgt ccattccagt attgtcatcg tgccctgccc
    18121 agctactgat ggtaatagac ggagtagaga caattagcca tgaatctcgg tattcattgg
    18181 tagaaccagt ttttccgacc caatctgctt ttcctagatt tccgtttaag cttgagatgg
    18241 catctttgaa tggtgtagtg atttttgcat tgatcacact gcgcatcatg tcattcatga
    18301 tcgatgccgt tgcttccgaa tagatccgga cagagttgct ttcgtgttta taaattacat
    18361 ttcctgcgtt atctgtgatg gaatcgatga tatagccttc ttcatagacg ccgccgtttg
    18421 ctaaagcctg aaatccattg gtctgttgaa gcgtcgtgac atctactgta cctaaaggag
    18481 cagattcaac gccccaattg tcatttgccg ggtagttcat ctttgccaag tagtgctcgt
    18541 aagcatattg tttcgatcca ccggaattca acacatcttg atataagtga taagccggga
    18601 tattgttgga ccattctagt gactcacgga cggtttggaa ggtgttcgat ccttcgttag
    18661 tagcattgac gattttctcg ccagcatttt ctccttcttg ccaagttgtt gcataatctg
    18721 atacacggga ttctgatccg ataagcccca tgtcgattgc aggtccataa acaagaactg
    18781 gtttgattga tgagccagct tgacgttctg tatcgaaggc atggttattt tgattctgac
    18841 tgtaattccg tccgccaacg aatccgtaga tgcggccagt tctgttatcc atcaatacat
    18901 ttccggtttc tacttgactg ccagcaccat catctaacat gtatccataa ttttctacgc
    18961 cattttgcaa tgcagcatag acatctttat cgattgtcga atggattgta tatcctttat
    19021 tttggatcgt ttgctgtgcc ttttgataat aagcgtcact gaccgatgaa tcagacatat
    19081 cagcgttgtc tttttcagcc aattgttgtg caatgattct tgttgcttca ttcatcacag
    19141 tataatacaa gaattctctg tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg
    19201 taaggtcata ggcttttgct tcttcgtatt gttctttcgt aatttggcct tcacgataca
    19261 tactgaataa aacaaaatct tttctggcaa ggccggcaga aagatcttct tttagcgcac
    19321 ctgtgttcgt gtatggacta tacgtgatcg ggctttgggg taatccagcg atataagcag
    19381 cttgcggcag cgtcacatct tttgcatcta caccaaagat tcctcttgca gcttcctgga
    19441 ctccagcaat gttttgtccc ttattattac gaccaaatgg tgagacattc aaatacgtag
    19501 caatgatctc atcttttgaa aagtattttt ctacttgtgc agaaagaatg atctcgtttg
    19561 ctttacgttt gaatgttgtt tcatctgtaa ggatctgctg tttgactaat tgctgtgtca
    19621 aagttgatcc accagaagag ccaataccag tagcttcaga tactaatgct cgtaaaacag
    19681 ctttaggaac gtaaccttgg tgtttatcga agtattcatc ctctgtgctg ataattgcag
    19741 ttttcaataa aggtgaaatt ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga
    19801 tttttgatat ctctgtatta tctgcataaa caatctttga cgtttctgta atgtttccta
    19861 gttctgtctg cagtgctttt ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat
    19921 agccagcgcc tattccaata cctagagctg cgaacatcaa aagaagaaca gtaataaaaa
    19981 cagtcaatga ttgaaaaact ctaaaaataa tcttaggaac aaaccatttt tctttttttc
    20041 ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca actaatggtt
    20101 ttatttcgct aattatagca aataatagaa caaaaaaaca gacaaggctg tttttggcag
    20161 gaatttcttt tttaaaagag ttaaaacgtg agaaaatgat cttttttata aaagagttct
    20221 taagcttttc tttagagaaa atttcagtta ctatggagaa cagggcaaag aaaaatcaat
    20281 cattttcttt gtatatcatc ttaccaatat ataggaattg ttagtaacaa ttgcctttca
    20341 aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa gaaaatgctg
    20401 ttaggagcag cagaagaagc agccagttat ggatcttcga cttttatgat ctatacaggc
    20461 gcccctcaaa acactagaag aaaagccatc gaagagatga atattgatgc cggaaaagcg
    20521 tacatggcag aaaacggtct aacaaatatc gtcgttcatg ccccttatat cattaatttg
    20581 gggaatacga tcaaaccaga aatgtttcct tttgccgtcc aatttctgag agaagagatt
    20641 gttcgtgcgg aagctttagg cgctactcaa attacacttc acccaggtgc tcatgtaggg
    20701 gcaggaacag aagccggaat tgctagaatt attgaaggct tgaatgaagt tttgacaaaa
    20761 gaacagacgg cacaaatcgc tttggagaca atggcaggta aaggtacgga aatcggtcga
    20821 acatttgagg aattagcgca aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt
    20881 acttttgaca cctgtcatac gaatgatgcc ggttataata ttaaagaaga cttttcaggt
    20941 gtaatggaag agtttgataa gatcatcggg ttagatcggc ttaaagtact ccatatcaat
    21001 gattctaaaa atccgcaagg ttcgcataaa gaccggcacg ccaatattgg ttttggaaca
    21061 atcggattcg acacgctgaa taagatcgtc catgatccaa tgtttgacaa tatttcgaaa
    21121 atcttggaaa ctccctatgt cggcaaagat aaaaagaaag cttacgcacc atatggaaaa
    21181 gaaatcgcca tgttcaaagc acaaatattc gatccagaag tgtttcctga gttattagca
    21241 gaagaagtga tactctaaga ttttctatct gacagcagca aaaaaggacc aggcacattg
    21301 ccttgtcctt ttttgtttaa gaaacaaagg agtgcttttt gcagtctttt tcatcatgca
    21361 tcttctcccc cctcttttta aatttagaca aacagggtat actggtagaa aagtaaaaaa
    21421 acgaaaagtt tcacaagcaa gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca
    21481 ctttaaaacc cttgatcaac caactaaaaa agaaacatac atactttatt cttggcatag
    21541 ccctattatt tacaattatt cgtttgtatt tatatagtaa agcaatctat ggaacagaca
    21601 tgtcggcaac ctatgatgac cagctgctga ttcgctatgc tcaaaatatt ttggacggta
    21661 aatggctagg ggagtatacg actacaacac tttccaaagg aatctcctac agcttatttt
    21721 tagtacttgc acataaactg catctttctt atgcagtttt gttcggcatg ttcagtattt
    21781 ttgccagtat attggttgca ctcgcgttta aaccgatcgt gaagaacaag tatcttcttt
    21841 taagtttcta tttttatttt ttgttttctc cgattagttt tacacatgaa tattccactc
    21901 gaacgtatcg taatgccctc gtgattccgg ctgttgagat cattatcgct tgtttactag
    21961 ctatttatta ccgtaaatat agttccagca aaaaaatgct gccgtggttt ttagggctag
    22021 ctgtttcttt tcctttcttt tggttcattc gcgaagattc catctggctt cttcctttta
    22081 ctgtagtcgc gttaggcatc agtttcgtcc agatcttgct aggacaagac ttttctttca
    22141 aagaagcaaa gaagttaaaa gaacgaccat ttctcctttc gaaacaagaa tggctgaaac
    22201 tttcattgtt tttactccct ctagtcggtt tctccatcgt ctatcagtgg atcgaatata
    22261 aaaacgagac aacatatagt attgccgcca tcaatgaccg ttcgagtggc gaatttggac
    22321 gtttgatgaa acagctgatc cgaatagatg acgaaacgaa cttaaataaa gaaaacagtg
    22381 agatatgggt ctcgcatgct gcattaaata aagcattaga cgcatctcct acatttcatg
    22441 ctgtttctac tcgtattgac gaattgtata cgactcatgc gtggacccaa ggtggaaaag
    22501 taaaagaatt accaggagat atcatttttt gggcattgcg agatgtggta agcgaatctg
    22561 gttattacca aaacaatgca aaagaaacca atgatttttg gaaaaaagtc aatcaagaat
    22621 tagaagaagc ctatcaaaag ggaactatca aaaagaaaaa agaattctat ctgatgggat
    22681 caggggatgg aaagacgatg aatgatctgc caatcgtatt tgcttttctg aaatcaggat
    22741 atgcctatgc atggcgatac aaagatttcc atcaaggggg gaattatagt ttcggtacgc
    22801 aagaacaagt cgaacaggcc caagaattat tgaatcttcc actaatggat aattggatcg
    22861 atagcagtga tccgagaaca cctgatttcc agttgacaaa aacagcaaaa gtcgcgaatg
    22921 tcatgataaa aatttaccaa gttctggcca ttcccgtgct gattgcttca atagcaggga
    22981 tattacttat gatactagga accgcatttg caaaagctga tcgtgcctat tatagttcct
    23041 ctttgattgt agtcaccgga ttaatactga cacaattggt ttttcttttc ggcgtctcgt
    23101 ggttttgttc ttatgcgcca gaaaaaaagg attatttttt aagtgtctac actggcgcag
    23161 gcgtgccatt aacgcaattc atcgtagtaa caagcatgct agttttcagt aaattgaatc
    23221 tgaaaaaagc tttatcttcc aaataaaaac atactaagat tagaggagaa catcattgac
    23281 gaatgatgtt ctcctctatt ttctattttt agattatagt gaagagtgaa agaaagttcg
    23341 aacaaagcct tgaggacta
//
LOCUS       DACCWV010000033        22852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCWV010000033 DACCWV010000000
VERSION     DACCWV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.033"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(290..1087)
                     /locus_tag="IVW84_001119"
     CDS             complement(290..1087)
                     /locus_tag="IVW84_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7917328.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IVW84_001120"
     CDS             complement(1084..2268)
                     /locus_tag="IVW84_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7917329.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IVW84_001121"
     CDS             complement(2293..3540)
                     /locus_tag="IVW84_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7917330.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IVW84_001122"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IVW84_001122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7917331.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IVW84_001123"
     CDS             5520..5834
                     /locus_tag="IVW84_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7917332.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IVW84_001124"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IVW84_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7917333.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IVW84_001125"
     CDS             6519..7163
                     /locus_tag="IVW84_001125"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7917334.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IVW84_001126"
     CDS             7178..7507
                     /locus_tag="IVW84_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917335.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IVW84_001127"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IVW84_001127"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7917336.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IVW84_001128"
     CDS             8495..9319
                     /locus_tag="IVW84_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7917337.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IVW84_001129"
     CDS             9312..9659
                     /locus_tag="IVW84_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7917338.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IVW84_001130"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IVW84_001130"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7917339.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IVW84_001131"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IVW84_001131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7917340.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IVW84_001132"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IVW84_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7917341.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IVW84_001133"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IVW84_001133"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7917342.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IVW84_001134"
     CDS             15316..16083
                     /locus_tag="IVW84_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917343.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="IVW84_001135"
     CDS             16073..18115
                     /locus_tag="IVW84_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917344.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="IVW84_001136"
     CDS             18396..18545
                     /locus_tag="IVW84_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7917345.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="IVW84_001137"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="IVW84_001137"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7917346.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="IVW84_001138"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="IVW84_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7917347.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="IVW84_001139"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="IVW84_001139"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7917348.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="IVW84_001140"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="IVW84_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7917349.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
BASE COUNT         7156 a         4112 c         4725 g         6859 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt agccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcgaaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggtgcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt tt
//
LOCUS       DACCWV010000034        22641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCWV010000034 DACCWV010000000
VERSION     DACCWV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.034"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            30..842
                     /locus_tag="IVW84_001141"
     CDS             30..842
                     /locus_tag="IVW84_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7917350.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            839..1639
                     /locus_tag="IVW84_001142"
     CDS             839..1639
                     /locus_tag="IVW84_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7917351.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1800..3068
                     /gene="rseP"
                     /locus_tag="IVW84_001143"
     CDS             1800..3068
                     /gene="rseP"
                     /locus_tag="IVW84_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7917352.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3136..4845
                     /locus_tag="IVW84_001144"
     CDS             3136..4845
                     /locus_tag="IVW84_001144"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7917353.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5057..9415
                     /locus_tag="IVW84_001145"
     CDS             5057..9415
                     /locus_tag="IVW84_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7917354.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9560..10033
                     /gene="rimP"
                     /locus_tag="IVW84_001146"
     CDS             9560..10033
                     /gene="rimP"
                     /locus_tag="IVW84_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7917355.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10056..11231
                     /gene="nusA"
                     /locus_tag="IVW84_001147"
     CDS             10056..11231
                     /gene="nusA"
                     /locus_tag="IVW84_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7917356.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11253..11546
                     /locus_tag="IVW84_001148"
     CDS             11253..11546
                     /locus_tag="IVW84_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7917357.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11543..11854
                     /locus_tag="IVW84_001149"
     CDS             11543..11854
                     /locus_tag="IVW84_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7917358.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11867..14173
                     /gene="infB"
                     /locus_tag="IVW84_001150"
     CDS             11867..14173
                     /gene="infB"
                     /locus_tag="IVW84_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7917359.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14200..14547
                     /gene="rbfA"
                     /locus_tag="IVW84_001151"
     CDS             14200..14547
                     /gene="rbfA"
                     /locus_tag="IVW84_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7917360.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14704..14877)
                     /locus_tag="IVW84_001152"
     CDS             complement(14704..14877)
                     /locus_tag="IVW84_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917361.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15325..17687
                     /locus_tag="IVW84_001153"
                     /pseudo
     CDS             15325..17687
                     /locus_tag="IVW84_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18055..18978
                     /gene="truB"
                     /locus_tag="IVW84_001154"
     CDS             18055..18978
                     /gene="truB"
                     /locus_tag="IVW84_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7917362.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18982..19923
                     /gene="ribF"
                     /locus_tag="IVW84_001155"
     CDS             18982..19923
                     /gene="ribF"
                     /locus_tag="IVW84_001155"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7917363.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20275..21060
                     /locus_tag="IVW84_001156"
     CDS             20275..21060
                     /locus_tag="IVW84_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7917364.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21073..21924
                     /locus_tag="IVW84_001157"
     CDS             21073..21924
                     /locus_tag="IVW84_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7917365.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22048..22500)
                     /locus_tag="IVW84_001158"
     CDS             complement(22048..22500)
                     /locus_tag="IVW84_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917366.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7682 a         3955 c         4953 g         6051 t
ORIGIN      
        1 ggaatctttt aagtatagga ggaaaaagaa tgttacgttt ttttccgcaa aaaaacaaat
       61 atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct cagcacattg
      121 ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca cgagttgctg
      181 gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca aggcttggag
      241 tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca aaagatgaag
      301 taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca gaattgatta
      361 aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa catacgcaag
      421 atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta ttgaattttg
      481 ctttgaatta tggaagcaga gcagagatcg tgactgctgt ccaagaaatt gccgaagaag
      541 tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct aagcatttaa
      601 tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt acaagtggag
      661 aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg tattttacaa
      721 aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc tcttatcaaa
      781 atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg gatcagtcat
      841 gaaacaacgt gtggtcacag cagccgtggc gctagtcttg tttattccga ttatatggat
      901 cggaggcata gcggttgagc ttacagcagc aatcttagca gtaataggag tttacgagct
      961 gtttcggatg aaaggattga cactcttaag ttttgaagga gttttatccg ccgttggtgc
     1021 agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga atgtaagtac
     1081 atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta tttctaaaaa
     1141 tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg tgggaatagg
     1201 tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat ttggtctatt
     1261 tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg gtcagcataa
     1321 gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg gtattggtag
     1381 tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac tatttaatca
     1441 tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac aatttggtga
     1501 tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa atatcttacc
     1561 aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc ctatcatgca
     1621 tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca taaaagacgc
     1681 aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta tggtaaaatt
     1741 atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag aaaaaagcta
     1801 tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt catgagtttg
     1861 gtcatttctt ctttgcgaaa cgatcaggaa tcctcgttcg cgaatttgct atcggtatgg
     1921 gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt cgcctattac
     1981 ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa atggcaccag
     2041 gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata aatttaagta
     2101 agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat ttagaagatg
     2161 agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc taccctgttg
     2221 atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg ccaaaagacg
     2281 tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct ggacccatga
     2341 ataactttat tttggcaatc ctgttattca tcattctggc atttatgcaa ggtggtgttc
     2401 aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg gcagaagcag
     2461 gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat tcatggaatg
     2521 atctgacaac agtcattacg aaaaatccgg ataaaacatt agactttaag atcgaacgag
     2581 aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat ggcgaaaaag
     2641 ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag atcattggag
     2701 ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga tcactattta
     2761 caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg tcttcagaag
     2821 cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca atgaatctcg
     2881 gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc ttgaatattt
     2941 ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg acattagctg
     3001 gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt caacgattct
     3061 ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt ttgaggaaat
     3121 aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg cgaagttcca
     3181 aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat ccgccaagta
     3241 tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa actgaaacgc
     3301 attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc tgccatcttg
     3361 ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa cttgtatcgt
     3421 ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga aacttttact
     3481 gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct ttatcaaatc
     3541 caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg aagagagttt
     3601 atcatgaaag atgcgtattc tttccatgca acggaagaaa gtttggacga gacatataaa
     3661 gattacgaaa aagcctacac cgaagtattc aagcgttgtg gcttagaatt tcgctcgatt
     3721 atcggagacg gtggcgcaat gggcgggaaa gactcaaaag aattcatggc gatctcagaa
     3781 atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa cttggagatg
     3841 gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt ggagaaagta
     3901 gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt tgaacctcaa
     3961 aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt tttggtacgc
     4021 ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga ctttttagaa
     4081 gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc tgtcggacca
     4141 gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga tttagcgaat
     4201 gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc agaacgcgat
     4261 ttcactgtac tttcttatga agatcttcgt tttgtacaag aaggagatcc ttcaccagac
     4321 aacaatggtg ttcttgcctt tactcgagga atcgaaatcg gacatatctt caaaatcggc
     4381 actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg agaaaaattt
     4441 gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat cgtagaacaa
     4501 cattcagatg aacaaggaat caactggcca aaaggaattg cgccatttga tttgcatatc
     4561 gtccaaatga atctaaaaga cgaataccaa acaaacctga cgaatgaact agaagaagcg
     4621 atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc aggagtgaaa
     4681 ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa aaaagcagtg
     4741 gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt acgtaaagaa
     4801 gaattggtga atactttacc aatcctcttg aaccaaggcg aataataagc gttaaaacac
     4861 agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc ttttaaaatt
     4921 ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa aaaacgagta
     4981 tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg tttctcatca
     5041 gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt tggaacaaat
     5101 ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa tggatagtgt
     5161 agtggtccat cgtaaatcgc gtttatggga atttacgctg catttttcca aaattttgcc
     5221 tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag atattgcaca
     5281 agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct tgcaagatta
     5341 ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc aagtattgaa
     5401 aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca gtacaggagc
     5461 tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg tcagttacgg
     5521 attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac gagtattgaa
     5581 actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag cagctgaaag
     5641 tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag ctcttgaagg
     5701 acccatccac atccagctag gacgaaatat tccagccgat gaaccaacga caccaatgat
     5761 cagtatcgtt gaagaagaac gaagagtcac acttgagggg tacgtattcg acaaagaagt
     5821 cagagaacta cgttcaaaac gcaagatctt gactttgaaa atcacagatt atacttcatc
     5881 atttatcgtc aaaaaattct cgaacggcga aaaagatgaa caagtatttg atgcgatcag
     5941 tgtcggtagc tggttaaagg tacgaggaag tattcaagaa gatacgtttg tccgtgattt
     6001 ggtcatgaat gcgcaagata tcatagaagt caaacataca cctcgcaaag attatgctcc
     6061 tgaaggggaa aaacgagtag agcttcatgt tcatagcaat atgagtacga tggatgcaac
     6121 aaacagcatc tctgatcttg tggcacaagc agggaaatgg ggacaccgag ctatcgccat
     6181 tacagaccac ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa aaaaagcagg
     6241 tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc caattgctta
     6301 taatgatgca catgaagcat tgagtgaagc cacatatgtc gtatttgacg tggaaacgac
     6361 aggattgtct gcagtatatg acacgatcat cgagctagcc gcagtcaaaa tgtataaagg
     6421 aaatgtgatc gaaagctttg atgaatttat cgatccagga catcctttgt cacgaacaac
     6481 ggtagatctg actggtatca cggatggaat ggttcgagga tcaaagtcgg aagaagaagt
     6541 attgcggatg ttccttgaat tttcaaaaga tacaattttg gtagcccaca acgcggcttt
     6601 tgatatgggg ttcttgaata ctagctacgc aaggtatggt attcctgaag cggccaatcc
     6661 agtcattgat acgttggaat tagctagata tctgtatcct caattcaaac gtttcggatt
     6721 aggtgtgtta tctaaaaaat ttggtgtcag cttggaacag catcaccgag caatctatga
     6781 tgctgaagca actggtcact tagcatggat ctttgtgaaa gaggcaatgg acaatcataa
     6841 catgctttat catgatcagc taaatgagca tatcggagaa ggagattctt acaaaagggc
     6901 tcgacctttc catgtaacga ttttagcaaa aaatcaggca ggactgaaag atttgttcaa
     6961 actgatttca atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac cacgctccca
     7021 attgaaaaag atgcgtgaaa atctattgat cggctcagct tgtgataagg gagaaatatt
     7081 cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat tctacgatta
     7141 tattgaagtt atgccaaaag cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa
     7201 tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa gtttggataa
     7261 aatcgttgtt gcaacaggta atgtacatta tctgaacgaa gaagatgcca tttaccgaaa
     7321 aattttgatc aattctatgg gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt
     7381 ccatttccga acaaccgatg agatgctaac tgcatttcac tttttagggg aagaaacagc
     7441 gaaagagatc gtagtagaaa atacgaataa aatcgcagat atctgcgaag aagtgattcc
     7501 agtcaaagat gaactttata caccgaagat tccaggttca gaagatgaaa tatcagaatt
     7561 gagttacaca aaagcaaaac aaatgtacgg agatccgtta cctgagatca ttcaaaaacg
     7621 attaaaaaaa gagttgaatt ctattaatgg taatggcttc tcggttattt atctgatcgc
     7681 ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta gtaggttcac gtggctctgt
     7741 tggctctagc tttgttgcga cgatgacagg aatcactgaa gttaatccgt tagcccctca
     7801 ttattattgt ccagagtgcc aatattccga gttttttgaa gatggtacgt atggttctgg
     7861 atttgacatg ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca aagacggtca
     7921 tgatattcca tttgagacat ttttaggatt ccatggagac aaagtgcccg atattgattt
     7981 gaacttctca ggtgattatc aagcagaagc ccataactat acgaaagtat tgtttggcga
     8041 agactatgtt taccgtgccg gaacgattgg tacagtcgcc gacaaaacag catatggcta
     8101 tgtaaaagga tacgaaaggg acaataatct gcagtttcga agtgcagaag tcgacagatt
     8161 agcaaaaggc gctactggag tcaaacggac aaccggccag catccagggg ggatcatcgt
     8221 tattcccgat tatatggatg tctatgattt tacgccgatc caatatccag cagacgatca
     8281 aaattcggaa tggaaaacga ctcattttga ttttcactcg attcacgata atgtattgaa
     8341 attagatatt ctagggcatg atgacccaac tgttattcgg atgcttcaag atttatcagg
     8401 gatcgatcca caaacgatcc ctacggatga cccggaagtg atgcggatat ttgccggtcc
     8461 tgaagtactt ggagtaagtc aggaacaaat ttattccaaa acagggacat taggtattcc
     8521 agaattcggc actcgttttg ttcgagggat gctagaagaa acgcatccta caacttttgc
     8581 agaactactg caaatttccg gactatctca tggtacagac gtatggttag gaaatgcgga
     8641 agaattgatt cgacgaggag atgcgacact ggcggaagta atcggctgtc gtgatgatat
     8701 tatggtttat ttgatccatg ctggattaga tagcgggatg gcatttaaaa tcatggaaac
     8761 tgttcgtaaa ggacaatgga acaagatccc tgatgaattg cgagaaacct atctatcggc
     8821 gatgaaagaa aataatgtgc ccgactggta tatcgattct tgttcaaaaa tcaaatatat
     8881 gtttccgaaa gcccatgctg cagcttacgt attgatggct cttcgtgtcg cttatttcaa
     8941 ggtttatttc cctattttgt attactgtgc atacttttct gtacgtgcag acgactttga
     9001 tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga
     9061 caaaggatta gatgcttccg taaaagaaaa gaatcaactg acagtgctgg agttagccaa
     9121 tgaaatgctg gaacgaggat tcaaatttgg aatgattgat ttgtataaat cggatgccgt
     9181 taactttgtt attgaaggtg atacgttgat cgcaccattt agagctgtcc caagtttggg
     9241 aaccaacgtg gcaaaacaaa tcgttgaagc aagaaaagac ggtccatttt tatccaaaga
     9301 agatcttgcc acccgcggta aagtttcaaa aacattgatc gaatatatga acgacaatgg
     9361 cgtattgaaa gatctaccag atgaaaatca attatcgtta ttcgatatgc tataagcatc
     9421 tgttttcctg tctgcttgta attttaagca gagtatggta tagttcttat aggtaatgat
     9481 actaactgtg agtgagcgga tttttcgctc actcttttta atggaattac gcacttaatt
     9541 ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa tggtgacacc
     9601 gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag aaggaaaaag
     9661 ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag aatgcgcctt
     9721 tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga ttcctcaagc
     9781 ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag aaagcgacta
     9841 tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag atggcgaaaa
     9901 acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga aaatacgcat
     9961 aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag ctcgcttagc
    10021 tatccaattt taatcacgga gaggaatcag aaaaaatgag taaagaaatg ttaaatgcgt
    10081 tagacgcttt agaagctgaa aaagggatct caaaagaaat cgtgatcgat gctttagaag
    10141 cagcattggt ttctgcatac aaacgtcact atggccaagc acaaaacgtc gaagtggaat
    10201 ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa agaagtaacg gaagaagtga
    10261 tggattcaca gttggaagtc tcattgaaag atgcactttt aatcaatcca gcatatgaga
    10321 tcggtgatac gatccgtttt gaagtaacac caaaagattt tgggcggatc gctgcgcaaa
    10381 cggctaaaca agttatttta cagcgtgtac gtgaagcaga aagaacgatt atttataacg
    10441 aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag gataaacgtt
    10501 atatttatgt caatcttggt aaaattgagg cagttctttc taaacaagat caaatgccaa
    10561 atgaatttta tcaaccacat gatcgaatca aagtttatgt ttcccgagta gaaaatacgt
    10621 ctaaaggtcc gcaagtattt gttagccgca gccatccaga cctattgcgc cgcttatttg
    10681 agcaagaagt accagaagtc tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag
    10741 ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat gcagtcggta
    10801 catgtgtcgg gccaaaggga caacgagttc aagcaatcgt caatgaatta aaaggcgaaa
    10861 acatggatat cgtcgaatgg aatgaagatc cagctgtatt cattgcaaat gcattgaatc
    10921 ctgcacaagt catggatgtg atcttcgatg agacaaatcc aaaggcctgc acagttgtcg
    10981 ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg acaaaacgca cgtttagctg
    11041 ctaaattgac taaccataaa atcgatatca aatcagaatc tgacatgacg gaattttatg
    11101 aaaatcaaga acaacaaaaa gaaacagaag aactgcatga tgaagcaatc gttcagtcag
    11161 atttaacaga cgatgaatat gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac
    11221 cagaagctta actttagtaa ggaggcaaag agatgaaaca aaggaaaatt cctttacgca
    11281 aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc acccgttcaa
    11341 aagaagggga agtatcgatc gatccaactg gaaaaatgcc tggacgagga gcgtatgtct
    11401 cacttgagcc ggaagaagta caacaagctt gggataaaaa aattctcgat cgagtactcg
    11461 aaacaacatt aacagatgag ttttatcaag aacttcttga ctatgtcact caccaaaaag
    11521 cacgtaaaga gttatttgga aaatgaatac agttaatcgg caaaaagcca tgaatctcat
    11581 cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca ttgctgaaat
    11641 ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat gcaagcgaga atacaagaaa
    11701 aaagataaaa gataagagtt catattatga agttccttgc tttgagctat tttctgaagc
    11761 agaaatcacg cagatgatcg gaaaacctcg caaggtattc ggaataatgg atgccggatt
    11821 tgcgaagaag accaaggagc taattgaagg ttaggaaggt gattgcatgg gcaacaaaag
    11881 aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca
    11941 acaacttggc atcgatgtga aaaaccatat gggtgcgatt actagcaatg atgagaagaa
    12001 attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac aagcaagcag
    12061 aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca
    12121 aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca
    12181 aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat accagcagcc aaaatcgtca
    12241 aggaacgagc caaggcggca atcaaaaccg tactaacaat aacaacaaac caaacaataa
    12301 caatcgcggc aaatttaata ataataaccg taatcgttat aataaaaaag gtaaaaaagg
    12361 caaacagcaa caatcaacta aaccagctgt accaccacgc aaattccgtg aattgccaga
    12421 tgtattagag tatactgaag gtatgaacgt agcagatatt gcgaaaaaaa ttcatcgtga
    12481 gccagcagaa atcattaaaa aattatttat gcttggtgtc atggtcaatc aaaaccaagc
    12541 actggataaa gatacgattg aactgttagc gacggactat ggcatggaac cacaagaaaa
    12601 agtccaagtt gacattgctg atatcgataa attcttcgag gcagaagaag tcgatccaga
    12661 taaattagtc tcacgtccgc cagtcgtaac gattatggga cacgtagacc atgggaaaac
    12721 aactttattg gatacattac gccattcacg tgtaacaagc ggcgaagccg gcggaatcac
    12781 acagcatatc ggtgcctacc agatcgatat tgatggtaaa ccaatcactt tcttggatac
    12841 accaggacat gcggccttta caagtatgcg tgcacgtgga gcaagtatca cagatatcac
    12901 gatcttagtt gttgctgcag atgatggcgt catgccacaa acagttgaag caatcaacca
    12961 tgccaaagct gcaggcgttc caatcatcgt cgctgtaaat aaaattgata aaccaggtgc
    13021 aaaccctcaa catgttatgc aagaattaag tgagtatgag ttaattcctg aagcatgggg
    13081 tggagaaacg atctttgttg agatctcagc gaaattcggc caaaacatcg aagaattgct
    13141 ggaaatgatc ctgttagtcg cagaagtaga agatttaaaa gcagatccaa cacaacgcgc
    13201 aatcggaaca gtgatcgaag cacgtcttga taaaggtaaa ggtcctgtgt caaccttact
    13261 tgtccaagaa ggaacacttc atgttggtga cccaatcgtt gtcggtaata cttacggacg
    13321 tgtccgtgta atgacaaacg atcttggccg tcgcgataaa gaagcaggac ctgcaacgcc
    13381 agtagaaatc actggcttga atgatgtacc tcaagcaggt gaccgtttcg tagtcttcga
    13441 agacgaaaag acagcacgtg cagcgggtga agaacgcgcg aaacgtgcat tattagaaaa
    13501 ccgtgctgcc acaagccgtg tgactttgga taacttattt gaaagcctga aagaaggcga
    13561 attgaaagaa gttaacgtaa tcatcaaagc agatgtacaa ggttcagcag aagcactggc
    13621 tgcatcattg aagaagattg atgtggaagg tgtccgtgta aaaatcgtcc attcagctgt
    13681 cggagcaatc aatgaaagtg atgtaacctt agcagcagcc agcaacgcta ttatcatcgg
    13741 tttcaacgtt cgtcctacac cgcaggcaaa agtgcaagct gatactgaag aagtcgatat
    13801 tcgtcttcac cgtatcatct ataaagccat cgaagaaatc gaaactgcga tgaaagggat
    13861 gctagaccct gaatttgaag aaaaaatcac cggtcaaatg atcgttcgtg aaaccttcaa
    13921 agtttctaaa gttggtacga ttgcaggtgc ctatgtaaca gaaggatata ttcgtagaga
    13981 tagcggtgtc cgtgtaatcc gtgacggaat cgtaatctat gaaggccaac ttgcaagtct
    14041 gaaacgattc aaagatgacg tgaaagaagt caaaatgggt tatgaatgtg gtgcaatgat
    14101 tgaaaaattc aatgacatca aagtagatga cgtaattgaa ggatttatca tggaagaagt
    14161 caaaaatgac taaaaactga taaaaggagg ccaatcatta tggctaacta ccgtgaccgc
    14221 cgagtcggtc aagaaatctt aaaagaagtc aatgatattt tgcgcaaaaa ggtccgtgac
    14281 cctcgtgtgg agaacgtaac gatcacagat gtgaaagtta cgggggactt acagcaagca
    14341 acgatctatt acagcatttt atctgactta gcatcagata agaaaacagc acaagatgga
    14401 ttgaataaag catcaggatt gattcgtcga gagttagggc acaatctaag tatttataag
    14461 acacctgaat tgttctttga acttgatgaa tcagttgtct atggcaacca tatcgatgaa
    14521 ttgcttcgcg acttgaacaa agactaatca aaaactcccg caaatcctgt gaaatcaggg
    14581 gttgcgggat ttttttgttt tcattaagac gtattaccta agagttccat gactttagtc
    14641 ttgcggcgtg tcagctaacc aaaaaagaga aaaagaaaag taaaaaattc gactttcttt
    14701 tttctagtag tatataaatt tttgcatata gataaagatc aagataccga taacggaagc
    14761 tagattgtaa tctattttag tgacagtcag gtatagccaa gtaactagaa gatcagtgga
    14821 acctgcacca taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa aaatcacgtt
    14881 gaagttattg atatttccga ttacgccgct tcgatttggt cacgatccaa attttggata
    14941 atccctgtag aaattagcat agtagctggg ataccaattc acatattgac aaagataaca
    15001 gagactttta cataaaacgg atcaatcagg aaaggaattg ctcgatccag ccccagtttt
    15061 ccagcaatgt gttaactggc ccagcgtcat tcagcaaatt atgcatgatg aggagcgaaa
    15121 tgaactgcgg aacagccatt gtgataacga caagtttttt ggatcaatgt aaaaaacgtc
    15181 agcgaaagga aaaatttcgt ggttttttat acctgttcat ttggctagat ataaaatcca
    15241 aacaaatgaa cagaagtgct tattaggttt caattgggag tatctgcgct agaataagag
    15301 taataatgaa aaggagtgtt atgtatggat tattctagta aagaatactt tgataaaatg
    15361 acggcttggt ggcgtgctgc taattatctt tctgttgggc aactttatct taaagataac
    15421 cccttactga gacgtacact gaaacccgaa gatgtcaaaa aacatccaat cggtcattgg
    15481 ggaacgattc ctggccaaaa ttttatttat gtccatttga accgagtcat caataaatat
    15541 gatttgaaca tgttttatat tgaagggcca ggacacggcg gacaagtcat ggtatcgaat
    15601 gcttatttag atggaagtta cacagagatc tatccagaag taacggaaga tgagacagga
    15661 atgcaaaaat tattcaaacg attttcattt cctggaggca ttgcttctca tgcagcacca
    15721 gaaacaccag gttctatcca tgaaggcgga gagcttggat actctctttc acatgcagtc
    15781 ggagctgtat tagacaatcc tgaagtaatc tctgctgtcg tgattggtga tggtgaagca
    15841 gaaaccggtc ctttagctgg aagctggttc tcaaatgtct ttatcaatcc agtgatagat
    15901 ggagctgttt taccgatcct tcatttaaat ggtgcaaaaa tagccaaccc aacgatcttg
    15961 gcacgtaaat cagatggaga attagcaaat tactttaacg gacttggctg ggaacctttc
    16021 tttattgaag gaaatgattc agaaaaatta aatcctgtta tggctgaaaa aatggatcaa
    16081 gcaatcgaga aaattaaatc catccaaaaa gaagctcgcc tgaagacagc aacagatgtc
    16141 gtgatgccaa aatggccagt attgatcgta cgcacaccaa aaggatggac cggtccaaaa
    16201 gaatgggacg gggaaccaat tgaaggaacc ttccgagcac accaagtacc gattcctgta
    16261 gatcaagaac atatggatca tgcagatgct ttactccgtt ggctgaaatc ctatgaacca
    16321 gaaaaattat ttgatgcaca aggacgtatt ttagaagaaa tcagagaaat cgcgcctact
    16381 ggtgaccaaa gaatggcaaa aaatcctatc accaatggtg gaatcgatcc aagaccgctt
    16441 attatgccag attggaaaaa atatactttg caatttgaaa aaccaggctc cgttaaagca
    16501 gaagatatga ccgaacttgg taaattcgtt cgcgagatca ttgagaaaaa tccagaaaat
    16561 ttccgtatat ttggaccaga tgaaacaaaa tcaaaccgat tgaatcaagt tttcaaaacg
    16621 acaaatcgcc aatggatgga aaaaatcgaa ccagaaaatg atgaatggct ttcgccatct
    16681 ggtcgcgtca tcgacagtca attatcggaa caccaagatg aagggttctt agaagggtac
    16741 gttctgactg gacggcatgg ttttttgcaa gttacgaatc attcttgcga gtggttgact
    16801 ctatgttgac tcagcacttc aaatggatgc ggaaatcaca tgacctttct tggcgaaatg
    16861 attatccttc gctaaatctc attgcaagct caacagtctt ccagcaagat cacaatggat
    16921 atagccacca agatccagga atattgacac atttggcaga aaagaaggcg gagttcatcc
    16981 gtgaatatct gcctgcggat gcaaatacat tgctcgcagt catggataaa gcattccgct
    17041 cgtcagaaaa aatcaatttg atcatctctt ccaaacatcc gcgtgctcaa ttttactcag
    17101 cagaagaagc agccgtctta gtgaatgaag gcttgaaaat cattgattgg gcttcgacag
    17161 caaaagagga ggaacctgaa ttggtcattg ctgctgcagg aacggaatct aacttagaag
    17221 cattagcagc tgtaactttg ctgctggaag aattcccaaa actaaaaatt cggttcatca
    17281 atgtcgtaga tttattgaaa ttacgtcatc caagtcaaga tccgcgtggg ttgagcgatg
    17341 aagaatttga tcaatatttt acaaaagaca aaccgatctt atttgctttc cacggttatg
    17401 aaacattggt ccgcacgatt ttctttgatc gtcataacca tcatttaatg attcatggct
    17461 acaaagaaaa tggagatatc acgacaccat ttgacatgcg tgtagtaaat gaacttgatc
    17521 gttatcattt ggcaaaagac gcagctttaa aaatcaaagg aagtcaagca gaagattttg
    17581 cgaaaaaaat ggatcagaaa ttacaagaac atcaaaacta tattcgtgaa aatggtattg
    17641 atctgccaga agtattggat tggaaatgga aaaatcttga ccaataaaat gataaaatcg
    17701 atcccgcaaa atagaatttt ttacattgat taagaaactg aaaaaataaa taaaaaaaac
    17761 ggaaaaaaga ctggaagaat ggccagtctt ttttttcgtt tttgggaagt gaaaaccaaa
    17821 ctatttacct gtaagccacc cataaatcca atcaaatggt gcaaagaaaa aatcttccat
    17881 tctattccct cctttaaaaa agcataacat gatttctatt tacgtgagca tgaattttgt
    17941 tacgttttta ttgccgaaaa ataaaaaccg ttcattatga tgtcaaattc gtcagtaatt
    18001 gatgcaatgt gttataataa ttcagttaaa tcaaaaatga aatggagaaa agacatggac
    18061 gggttattac ctttatggaa agaacgcgga atgacaagtc atgattgcgt attcaaatta
    18121 agaaaaatct tacatacgaa aaaaatcgga catgggggta cgcttgaccc agacgtagac
    18181 ggtgtgttgc ccatttgtat tggaaaagcc acaaaagtta tcgaatattt gacagattct
    18241 gggaaaatct atgtaggcga gataactcta ggtttcagta caacgacgga agatcgatcc
    18301 ggagaacggg tagaaacaaa accagtagat atgtctttat ctgaaaaaaa aatagatgaa
    18361 gtgatggctt catttattgg tgagatcacc cagataccac caatgtactc agcggtaaaa
    18421 gtcaacggta agcggttata tgaatatgcc cgcaataatg aaacagtgga gcgtcctgtt
    18481 agaaaggctc agatttaccg ttttatccgg acaagtgaat tgacaagaga cgaagtgaca
    18541 ggaaccattt cttggcgctt cgaagtagaa tgcgggaaag gaacctatgt ccgcacatta
    18601 gctgtagata caggcaagaa acttggttac cctgcgcaca tgtctgatct gacccgaaca
    18661 gcaagtgccg gtatgtccca gaaggatgct ctgacattag aagaagtagc tcaagctgtg
    18721 aaaaatggga caatcgatga ttgtttgctg ccaatagaaa aaggagtgga acggttcaaa
    18781 cgagtagata ttactgatga agtatggcag aaagtaaaaa acggtatgag acttgattat
    18841 actgtatttg cgctcccaga aatgccgtca gaagaaattg ctttgtttta taaaggaaaa
    18901 gttgtcagta tttatcagcc aaacccgaaa gaaaaaaaca aattaaaacc tagtaaagta
    18961 ttgagaaatg aggaataacg aatgaatatc atcaagatca gacatcctta ccaatcagaa
    19021 caaattccag aagacgatgt cgttttagtg ctgggatttt tcgacggcgt gcatatcgga
    19081 caccagaaag tcataaagac aggtatggaa attgcacaaa aagaaggatt gaaattagca
    19141 ttgatgactt tcaatcagca tccatctatt gtctttaaaa agatcgatcc tagcagcgtg
    19201 aaatatttga ctagccttaa gcaaaaagaa gaaaaaatgg ccgctttggg tatagattat
    19261 ttatatgaag ttgattttac ttcttctttt gctcatttag caccgcagga atttgttgat
    19321 caatacatcg tagggttaca tgcaaaatat gctgtttccg gttttgatta tacgtatgga
    19381 ccaaaagaca ttgcagatgt cgctcatttt cctgaatatg ccaaaggaag attcaaaatc
    19441 gtcacagttt ctaaagaaga agaacaaggt gaaaaaatca gctctacccg tattcgtgga
    19501 ttactagaca gcggacaagt aaaagaagca gcaaaactat taggttgcac atatgaggta
    19561 gaaggggtag ttgtccatgg cgatgcacga ggcagactac taggattccc tacagcaaat
    19621 gtcaaagtaa agagcacggt tcatttgcct aaagaaggtg tttatgtgtc ggaaataaaa
    19681 attggggaaa catggtatcc agccatgggt tcaattggac ataatgatac attcggtgaa
    19741 ggacgggagc tgactgtaga gttgtatatt ttggacttta atcaagatat ctatggtgaa
    19801 catgtcgcgg tacgctggaa tcaattttta agagatcaag tgaaattttc tagtgtagag
    19861 gaattgattg aacaactcaa ggaggatgaa caagcaacag ctgaatattt ttctcagcaa
    19921 taaaacatta tgcataaaat ctcttgttta tgtataacta gcaattacaa taaaatgggt
    19981 gataaataag cccaggaaag gggctgtgac aaaagtcttg tcacagcctt tttttccaaa
    20041 taaatggttt ttcagaagta gcttcttcgg aaataagccg aaatttcttg aagctgacca
    20101 cttctgtcac aacctctttc tttatagaaa taaaaggaca ctagcaattc atgaaaacga
    20161 ttgctagagc gtatcatttg ctttagacta tatatcttgg tgtacactca ctttaaggcg
    20221 gaaataaaag ttatccgtaa taacaggttt aggtatgaaa aaggagtggg tactatgtac
    20281 aaggaattag aaaacaaagt cgcagtagta actggtggct caaaggggat tggaacagcg
    20341 attgctcaac gattcgggaa agaaaaaatg aatgtagttg tcaactataa ctcggataaa
    20401 gaaggagcag aaaaagcagt caaagcaatc gaagaggcag gcggaaaagc agtctctgta
    20461 caagcagatg tcagttctga agaaggaata cagcgtttac ttgatacggc tttagaaaat
    20521 tttgggacac tggacgtttg ggttaataat gctgggatgg aaaatcagca tccaacccat
    20581 gaacttagcc ttgaagattg ggaaagagtg ttaaaagtca atttgactgg cgtttttatc
    20641 ggaacgaaag cggctttaaa ttactttaca gagaataata aaaaaggaaa tatcatcaat
    20701 atgtcttctg ttcatgaaca gatcccatgg ccaacgtttg ctcattacgc tgcttcaaaa
    20761 ggcggggtaa aattatttac cgaaacggta gcaatggagt atgcaccgaa gggaatcaga
    20821 atcaatagta taggccctgg tgctatccgt acacctatta atgcggcaaa atttgatgat
    20881 cctgaacaaa aagaactctt ggaaagtatg atcccactag gacgcgtagg tgaaccaaaa
    20941 gaaatagcgg cgacagctgc atggttggct tcagacgaat ccagttatgt tacaggtatt
    21001 acactatttg cagatggtgg gatgacttta tatccttctt tccaaggtgg aaaaggataa
    21061 gggtgaataa aaatgggttt attgattgca ttgatcccgg cagttggctg ggggatccag
    21121 ccactgattt tgaaaaagat tggtggcaga ccaacaaatg agatattagg aactggtatt
    21181 ggagcggtgc ttgtcggctt actcgttcaa ttattcgctt ctccagctgg tatttcagtg
    21241 actacatttt tgattagttt actttctggt gcattttggg ttattggaca aacaggacag
    21301 tatacatctt tcaacttgat tggtgtttct aaaacgatgc caatttcgac tgcattacag
    21361 ttagtcggaa cctcgttgat tggtgtcttc gcttttggag aatggtctgg aagtatgggg
    21421 aaaatcattg gagcagcagc aatcattctt cttgtatttg gttctgcttt aacggcaatc
    21481 agtgatgatc ctggaaaaaa aggcggtatc acaaaaggaa ttagtctatt agcatcaaca
    21541 agtattggtt attgggtata cagtgcattg cctaaactag ttgatgcaag cggactttcc
    21601 attttctttc cgcaaatgat cggtgtcttt ttaggggcag tgatttatgt gattttccgt
    21661 aaaccaaccg cattttctga agacaagagt tggaaagcaa cgatcgttgg agccatcttt
    21721 agtgtgtcag cattggctta tattttttca gcaaatgaaa atggggttgc tactgcatat
    21781 atcattacac agctaaacgt agttatctct acattaggcg gtatgtacat cctgcatgaa
    21841 aataagacac caaaagaaaa aaaattaacg attatcggat tattgctgat cgtagtagga
    21901 agcatggtca cggtctttat ttgaggataa agatggtaaa atttcattta ttaaaaaaat
    21961 atttgtttga accaaacagt aagtacttat aaaaaaatag cgctagaatt tccaacatct
    22021 ctggaatttc tagcgctttt taattgttta agatacaagg gaaatatctg tctggatcaa
    22081 taatttttca ttgatccatt gactaaagtt tacgacataa ttgaagttac caccgattag
    22141 tatctccgca tgattgtctt tataaagatc gtattgtaaa agacggtctt ttttatttgc
    22201 gtaaacatcg ttatggtact ggactaattg ttctcggaat tgattgattg ctgtaaaatc
    22261 acgatattga aggttgaaac gcatcgtgat tggtgcattt agcatgaatc gctgcatttc
    22321 ttttgctgct tcctctgctt gatctccgta taggaactca aaaagattac gttcagaaaa
    22381 tttccattgc ccgccaacct ttgttccttt tagttttcct tcatgaagat aatttctgat
    22441 agaacgtgta gtaacaccta attgttcggc taattcattt actgagtaaa aatctttcaa
    22501 tgttcaccct cctatgtgaa tggttctact gacttagttg atatttgctt aatcacgaaa
    22561 tgtagaaaga acgattttaa ttaaggtcat tgtaaaaatc aagtcagaaa attctaactt
    22621 ttatttctct aaagaaattc g
//
LOCUS       DACCWV010000035        22637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCWV010000035 DACCWV010000000
VERSION     DACCWV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.035"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(226..1113)
                     /locus_tag="IVW84_001159"
     CDS             complement(226..1113)
                     /locus_tag="IVW84_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917367.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="IVW84_001160"
     CDS             complement(1110..1979)
                     /locus_tag="IVW84_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917368.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="IVW84_001161"
     CDS             complement(2116..3402)
                     /locus_tag="IVW84_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7917369.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="IVW84_001162"
     CDS             3675..4253
                     /locus_tag="IVW84_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7917370.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="IVW84_001163"
     CDS             4253..5182
                     /locus_tag="IVW84_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7917371.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="IVW84_001164"
     CDS             5179..6915
                     /locus_tag="IVW84_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7917372.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="IVW84_001165"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="IVW84_001165"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7917373.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="IVW84_001166"
     CDS             complement(7811..8320)
                     /locus_tag="IVW84_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7917374.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="IVW84_001167"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="IVW84_001167"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7917375.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="IVW84_001168"
     CDS             complement(9621..10712)
                     /locus_tag="IVW84_001168"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7917376.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="IVW84_001169"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="IVW84_001169"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7917377.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="IVW84_001170"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="IVW84_001170"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7917378.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="IVW84_001171"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="IVW84_001171"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7917379.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14675..15649)
                     /locus_tag="IVW84_001172"
     CDS             complement(14675..15649)
                     /locus_tag="IVW84_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7917380.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15630..17513)
                     /locus_tag="IVW84_001173"
     CDS             complement(15630..17513)
                     /locus_tag="IVW84_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7917381.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17531..19756)
                     /locus_tag="IVW84_001174"
     CDS             complement(17531..19756)
                     /locus_tag="IVW84_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7917382.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19871..20905
                     /locus_tag="IVW84_001175"
     CDS             19871..20905
                     /locus_tag="IVW84_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917383.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20972..21985)
                     /locus_tag="IVW84_001176"
     CDS             complement(20972..21985)
                     /locus_tag="IVW84_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7917384.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22063..22251)
                     /locus_tag="IVW84_001177"
     CDS             complement(22063..22251)
                     /locus_tag="IVW84_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7917385.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6450 a         4863 c         4095 g         7229 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac ctagtataga
    14461 cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc tgatagttaa
    14521 ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat gaacaaaagt
    14581 atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc tttgtcttgt
    14641 aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag ccgatttcat
    14701 ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag ctattgatac
    14761 ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt
    14821 cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa
    14881 atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta tccgtattat
    14941 ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg ccgcattctt
    15001 ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct tcgacaccac
    15061 cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa aattttgctt
    15121 tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct ttttgtccat
    15181 aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa attgtcactt
    15241 gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt
    15301 cattacttga atattgatta tttactggag caatcggcaa atcgatcttt ttattgtcca
    15361 ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac ataccggatt
    15421 taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg
    15481 tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg cctcttgtat
    15541 aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg ccgaagacga
    15601 ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata ataatatctt
    15661 acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat
    15721 tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg
    15781 attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc tccttggtac
    15841 atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga attccccatg
    15901 tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag gtagtcaatg
    15961 atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc ttcgaatgct
    16021 ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt tttgaagtaa
    16081 tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga ccaaaaaaga
    16141 atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc
    16201 tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc cattttttgc
    16261 catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc ttggtcgcat
    16321 aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt gatctttgca
    16381 ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg tccttttttc
    16441 ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt gaagtagatg
    16501 attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata ctccgttact
    16561 cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg aaaatgcccc
    16621 cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt taacggatat
    16681 tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag ctgactgttt
    16741 ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca tttgtcatct
    16801 aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac taagaagaca
    16861 tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc agctgtactt
    16921 cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt taaatcaaat
    16981 tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa ccaaataaac
    17041 attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag accttcttct
    17101 aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga aaagatccct
    17161 tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg tccctcccac
    17221 ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat atgactagga
    17281 acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt ttttggtcgt
    17341 accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg tttaagggag
    17401 gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg tgcatttgat
    17461 tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt catacttttt
    17521 tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat
    17581 cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc ttcgaggtct
    17641 gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg aacgctggtt
    17701 gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta ggagacacaa
    17761 acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa tggccatact
    17821 ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc tttttttctt
    17881 caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata gctgtttctc
    17941 ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct gatacatgag
    18001 ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc atacgctcca
    18061 ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac ccggcatcaa
    18121 accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca gttgtcaaat
    18181 gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct tgtctctgtg
    18241 ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat ttgatgtagt
    18301 caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc cgaacatcta
    18361 accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta cctggctcct
    18421 gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc atttcaggtt
    18481 caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt tcacgtaatc
    18541 catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg tccgcatctc
    18601 tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc tttgcttcat
    18661 caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc cagttattga
    18721 ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca cgtagcaact
    18781 catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg atcatttcag
    18841 gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg ttgatcccac
    18901 cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg ttgccggaat
    18961 aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga tgaactaaag
    19021 caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat acttttgttc
    19081 cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa
    19141 tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc gctttttcta
    19201 gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc actgctctat
    19261 cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa
    19321 tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag ccttcgatct
    19381 ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag tctgatccat
    19441 tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga gaagagtatt
    19501 cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca tttcctgaaa
    19561 atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact ggtttgccaa
    19621 aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa agaaatgtat
    19681 ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta tctgtttttc
    19741 tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact tttttttata
    19801 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    19861 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    19921 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    19981 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    20041 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    20101 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    20161 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    20221 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    20281 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    20341 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    20401 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    20461 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    20521 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    20581 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    20641 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    20701 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    20761 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    20821 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    20881 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    20941 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    21001 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    21061 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    21121 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    21181 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    21241 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    21301 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    21361 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    21421 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    21481 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    21541 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    21601 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    21661 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    21721 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    21781 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    21841 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    21901 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    21961 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    22021 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    22081 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    22141 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    22201 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    22261 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    22321 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    22381 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    22441 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    22501 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    22561 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    22621 atgtagacta actaaaa
//
LOCUS       DACCWV010000036        22319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCWV010000036 DACCWV010000000
VERSION     DACCWV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.036"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            5..403
                     /locus_tag="IVW84_001178"
     CDS             5..403
                     /locus_tag="IVW84_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7917386.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IVW84_001179"
     CDS             387..1082
                     /locus_tag="IVW84_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7917387.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="IVW84_001180"
     CDS             complement(1676..2563)
                     /locus_tag="IVW84_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7917388.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="IVW84_001181"
     CDS             2748..3587
                     /locus_tag="IVW84_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7917389.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="IVW84_001182"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="IVW84_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7917390.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="IVW84_001183"
     CDS             5055..5411
                     /locus_tag="IVW84_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917391.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="IVW84_001184"
     CDS             5414..6571
                     /locus_tag="IVW84_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917392.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="IVW84_001185"
     CDS             complement(6597..7037)
                     /locus_tag="IVW84_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7917393.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="IVW84_001186"
     CDS             7197..7703
                     /locus_tag="IVW84_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7917394.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9783
                     /locus_tag="IVW84_001187"
                     /pseudo
     CDS             7871..9783
                     /locus_tag="IVW84_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            9788..10789
                     /locus_tag="IVW84_001188"
     CDS             9788..10789
                     /locus_tag="IVW84_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7917395.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10991..11197)
                     /locus_tag="IVW84_001189"
     CDS             complement(10991..11197)
                     /locus_tag="IVW84_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7917396.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11843..12691)
                     /locus_tag="IVW84_001190"
     CDS             complement(11843..12691)
                     /locus_tag="IVW84_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7917397.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12855..13757
                     /gene="gnd"
                     /locus_tag="IVW84_001191"
     CDS             12855..13757
                     /gene="gnd"
                     /locus_tag="IVW84_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7917398.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13759..14760
                     /locus_tag="IVW84_001192"
     CDS             13759..14760
                     /locus_tag="IVW84_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7917399.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14757..15041
                     /locus_tag="IVW84_001193"
     CDS             14757..15041
                     /locus_tag="IVW84_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7917400.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15074..15871
                     /locus_tag="IVW84_001194"
     CDS             15074..15871
                     /locus_tag="IVW84_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7917401.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15885..16718
                     /locus_tag="IVW84_001195"
     CDS             15885..16718
                     /locus_tag="IVW84_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7917402.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16736..17593
                     /locus_tag="IVW84_001196"
     CDS             16736..17593
                     /locus_tag="IVW84_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7917403.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17615..18124
                     /locus_tag="IVW84_001197"
     CDS             17615..18124
                     /locus_tag="IVW84_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7917404.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18161..18562
                     /locus_tag="IVW84_001198"
     CDS             18161..18562
                     /locus_tag="IVW84_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917405.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18674..19027
                     /locus_tag="IVW84_001199"
     CDS             18674..19027
                     /locus_tag="IVW84_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917406.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19061..19504
                     /locus_tag="IVW84_001200"
     CDS             19061..19504
                     /locus_tag="IVW84_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7917407.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19521..20606
                     /gene="uxuA"
                     /locus_tag="IVW84_001201"
     CDS             19521..20606
                     /gene="uxuA"
                     /locus_tag="IVW84_001201"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7917408.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20599..21312
                     /locus_tag="IVW84_001202"
     CDS             20599..21312
                     /locus_tag="IVW84_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7917409.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22064..22264
                     /locus_tag="IVW84_001203"
     CDS             22064..22264
                     /locus_tag="IVW84_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7917410.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGTQATNIVKA"
BASE COUNT         7684 a         3429 c         4626 g         6580 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat
     9721 tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg
     9781 taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat
     9841 tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag
     9901 aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca
     9961 aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa
    10021 taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat
    10081 gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct
    10141 atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa
    10201 acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa
    10261 agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag
    10321 tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc
    10381 gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc
    10441 tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc
    10501 ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag
    10561 acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac
    10621 gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt
    10681 agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa
    10741 acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat
    10801 gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca
    10861 agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg
    10921 caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa
    10981 attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg
    11041 cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata
    11101 ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac
    11161 tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttatag
    11221 ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa
    11281 atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca
    11341 atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa atatgggaat
    11401 ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg atgtatccta
    11461 aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca aatgataacc
    11521 taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa aatttttttt
    11581 aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta
    11641 ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt
    11701 ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct
    11761 tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt
    11821 tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag
    11881 cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca
    11941 aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag
    12001 aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa
    12061 taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg
    12121 attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca
    12181 tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat
    12241 gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag
    12301 attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt
    12361 catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg
    12421 ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg
    12481 ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag
    12541 agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg
    12601 gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg
    12661 ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta
    12721 tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa
    12781 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
    12841 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
    12901 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
    12961 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
    13021 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
    13081 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
    13141 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
    13201 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
    13261 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
    13321 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
    13381 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
    13441 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
    13501 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
    13561 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
    13621 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
    13681 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
    13741 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
    13801 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
    13861 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
    13921 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
    13981 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
    14041 caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat
    14101 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
    14161 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
    14221 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
    14281 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
    14341 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
    14401 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
    14461 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
    14521 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
    14581 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
    14641 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
    14701 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
    14761 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
    14821 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
    14881 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
    14941 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
    15001 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
    15061 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
    15121 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
    15181 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
    15241 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
    15301 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
    15361 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
    15421 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
    15481 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
    15541 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
    15601 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
    15661 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
    15721 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
    15781 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
    15841 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
    15901 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
    15961 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
    16021 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
    16081 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
    16141 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
    16201 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
    16261 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
    16321 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
    16381 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
    16441 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
    16501 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
    16561 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
    16621 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
    16681 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
    16741 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
    16801 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
    16861 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
    16921 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
    16981 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
    17041 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
    17101 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
    17161 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
    17221 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
    17281 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
    17341 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
    17401 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
    17461 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
    17521 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
    17581 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
    17641 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
    17701 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
    17761 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
    17821 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
    17881 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
    17941 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
    18001 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
    18061 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
    18121 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
    18181 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
    18241 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
    18301 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
    18361 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
    18421 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
    18481 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
    18541 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
    18601 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
    18661 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
    18721 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
    18781 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
    18841 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
    18901 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
    18961 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
    19021 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
    19081 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
    19141 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
    19201 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
    19261 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
    19321 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
    19381 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
    19441 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
    19501 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
    19561 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
    19621 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
    19681 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
    19741 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
    19801 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
    19861 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
    19921 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
    19981 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
    20041 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
    20101 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
    20161 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
    20221 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
    20281 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
    20341 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
    20401 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
    20461 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
    20521 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
    20581 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
    20641 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
    20701 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
    20761 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
    20821 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
    20881 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
    20941 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
    21001 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
    21061 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
    21121 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
    21181 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
    21241 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
    21301 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
    21361 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
    21421 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
    21481 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
    21541 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
    21601 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
    21661 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
    21721 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
    21781 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
    21841 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
    21901 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
    21961 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
    22021 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
    22081 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
    22141 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
    22201 agtgtcttac gatgttgaag atggtcaacg tggcactcaa gcaacaaaca ttgttaaagc
    22261 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaa
//
LOCUS       DACCWV010000037        22194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCWV010000037 DACCWV010000000
VERSION     DACCWV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.037"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(87..605)
                     /locus_tag="IVW84_001204"
     CDS             complement(87..605)
                     /locus_tag="IVW84_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7917411.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(607..1224)
                     /locus_tag="IVW84_001205"
     CDS             complement(607..1224)
                     /locus_tag="IVW84_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7917412.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1221..2075)
                     /locus_tag="IVW84_001206"
     CDS             complement(1221..2075)
                     /locus_tag="IVW84_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917413.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2072..2983)
                     /locus_tag="IVW84_001207"
     CDS             complement(2072..2983)
                     /locus_tag="IVW84_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917414.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3024..4358)
                     /locus_tag="IVW84_001208"
     CDS             complement(3024..4358)
                     /locus_tag="IVW84_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7917415.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4566..5396
                     /locus_tag="IVW84_001209"
     CDS             4566..5396
                     /locus_tag="IVW84_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7917416.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5640..5889)
                     /locus_tag="IVW84_001210"
                     /pseudo
     CDS             complement(5640..5889)
                     /locus_tag="IVW84_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6080..6274)
                     /locus_tag="IVW84_001211"
     CDS             complement(6080..6274)
                     /locus_tag="IVW84_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917417.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6779..6964)
                     /locus_tag="IVW84_001212"
     CDS             complement(6779..6964)
                     /locus_tag="IVW84_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917418.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7814..8155)
                     /locus_tag="IVW84_001213"
     CDS             complement(7814..8155)
                     /locus_tag="IVW84_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7917419.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8155..8385)
                     /locus_tag="IVW84_001214"
     CDS             complement(8155..8385)
                     /locus_tag="IVW84_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7917420.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8672..9010
                     /locus_tag="IVW84_001215"
     CDS             8672..9010
                     /locus_tag="IVW84_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917421.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9215..9454)
                     /locus_tag="IVW84_001216"
     CDS             complement(9215..9454)
                     /locus_tag="IVW84_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7917422.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9430..9777)
                     /locus_tag="IVW84_001217"
     CDS             complement(9430..9777)
                     /locus_tag="IVW84_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7917423.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10509..11090)
                     /locus_tag="IVW84_001218"
     CDS             complement(10509..11090)
                     /locus_tag="IVW84_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7917424.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11273..11899)
                     /locus_tag="IVW84_001219"
     CDS             complement(11273..11899)
                     /locus_tag="IVW84_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7917425.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11921..13252)
                     /locus_tag="IVW84_001220"
     CDS             complement(11921..13252)
                     /locus_tag="IVW84_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7917426.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13407..13808)
                     /locus_tag="IVW84_001221"
     CDS             complement(13407..13808)
                     /locus_tag="IVW84_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7917427.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13894..14100)
                     /locus_tag="IVW84_001222"
     CDS             complement(13894..14100)
                     /locus_tag="IVW84_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917428.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14195..15697)
                     /locus_tag="IVW84_001223"
     CDS             complement(14195..15697)
                     /locus_tag="IVW84_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7917429.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15715..16170)
                     /gene="argR"
                     /locus_tag="IVW84_001224"
     CDS             complement(15715..16170)
                     /gene="argR"
                     /locus_tag="IVW84_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7917430.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16268..16885)
                     /locus_tag="IVW84_001225"
     CDS             complement(16268..16885)
                     /locus_tag="IVW84_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917431.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17106..18479
                     /locus_tag="IVW84_001226"
     CDS             17106..18479
                     /locus_tag="IVW84_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7917432.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18554..19666
                     /locus_tag="IVW84_001227"
     CDS             18554..19666
                     /locus_tag="IVW84_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7917433.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19738..21375)
                     /locus_tag="IVW84_001228"
     CDS             complement(19738..21375)
                     /locus_tag="IVW84_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7917434.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21543..22151)
                     /locus_tag="IVW84_001229"
     CDS             complement(21543..22151)
                     /locus_tag="IVW84_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7917435.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6464 a         4529 c         3871 g         7330 t
ORIGIN      
        1 tgaataaaca actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc
       61 actcatgctt tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca
      121 cttcctaaaa tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc
      181 gaataagcga tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg
      241 aacaacgcag ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc
      301 ttcggtttac ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt
      361 gtcgtttttt tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg
      421 gctagaccat attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa
      481 ataaaaccac cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc
      541 gttttccgtt gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc
      601 aacatgttat tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac
      661 ttgaaaataa aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca
      721 gaatgcgaca agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt
      781 aaaaaacaag aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat
      841 tttgtaacta aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt
      901 atagccaatt cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa
      961 ataaacactt gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt
     1021 ccagaatagt gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag
     1081 aactgttcga cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag
     1141 ctgtaaatgg ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt
     1201 tttaccaatc gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg
     1261 aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct
     1321 gccatcagat aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt
     1381 aacgtgtatt tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct
     1441 gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa
     1501 tgactccaaa tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta
     1561 ttgatcgata agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat
     1621 ggaacgatca ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga
     1681 gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc
     1741 tttttcccag gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca
     1801 ttaccgatca ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga
     1861 aaggcggcaa tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga
     1921 ggataacgaa cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga
     1981 atgatcaaaa tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc
     2041 cgattcacac tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga
     2101 cccacttagg tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga
     2161 tcattgacat agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat
     2221 gcagattata aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat
     2281 aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa
     2341 taggagtgat taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga
     2401 tttctgctga ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca
     2461 ttgctcctcc agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat
     2521 caaacagcca agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg
     2581 cagatggaga aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg
     2641 gtaaatagaa agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg
     2701 ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt
     2761 tgtataacga tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac
     2821 caatgaaatt catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa
     2881 gcataggccc tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg
     2941 cccatcttgc ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct
     3001 gtacctagac agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt
     3061 tgtgcatctg ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt
     3121 tcattcacta atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt
     3181 ttaaagtttt cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta
     3241 ttcactaaat tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat
     3301 ttttcttgta cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct
     3361 tttgctccgt atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct
     3421 tcttcttttt ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc
     3481 aaactagagt aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga
     3541 tcagatacac cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa
     3601 tcttgccagt cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact
     3661 ttttcatctt tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc
     3721 cctaaattcc ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct
     3781 agtttacgtg ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt
     3841 ccagcttctt taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg
     3901 atcgcataag gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta
     3961 gaagtgtctt tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg
     4021 gcaacggaat ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat
     4081 aaagatttag tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt
     4141 tgtgattgat tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc
     4201 caaaaggacc agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct
     4261 gcacttgaac tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct
     4321 gaacttgcca gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg
     4381 taaccgatgc cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag
     4441 gtatgattct aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc
     4501 tttgaatttt ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa
     4561 ttttgatgtt tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc
     4621 cattaatcgc gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag
     4681 ctatgcagta cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc
     4741 tgatggacat tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc
     4801 gtttattaga gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa
     4861 tcggtgccat tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc
     4921 gtcctgctga cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact
     4981 tgttagcaga ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac
     5041 gtatttacga aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa
     5101 gagatagcta tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag
     5161 gttcaatctt agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg
     5221 acttctataa cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag
     5281 cactggatgg cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac
     5341 caaaattaga accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat
     5401 ctacccgtag aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt
     5461 cattaatcat aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac
     5521 cggccagcgc ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc
     5581 agttttgccc acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct
     5641 tactctcgta cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac
     5701 tttttgatgg tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag
     5761 tgtcgatacc caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt
     5821 ggctcgatat tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta
     5881 tttacacatc tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca
     5941 agtggttttg tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa
     6001 aaacccgctg cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga
     6061 tctcttacgg caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa
     6121 gtccaagaaa taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt
     6181 caacagaaat cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat
     6241 gctttttata gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa
     6301 ttaatcacaa attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac
     6361 tcttcgactt tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg
     6421 tacctctaaa tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc
     6481 ccatccagta tacataacct tgatacttcg accacttata tatcgaacac acaaatacct
     6541 atccattgca ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa
     6601 gataggactt actcttagta aagtatctga actattaatt tttaaactat catttttatc
     6661 ctcaactaca tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa
     6721 ggaatcggga attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc
     6781 atatctttat tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta
     6841 atgtatcatt aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc
     6901 caaccaacat ataccctata aaagctaata agatggttag gaatccttta gaatttttac
     6961 tcattcatag acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat
     7021 tgacaaaaag tgactcaaaa taaaaaaaac cttacattat actggaatga actgtattga
     7081 tagtgtacct aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa
     7141 ttatacggga tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat
     7201 aattgtcgct ttgagattct actttggttc actatctgac ccaattgcac attttcgatc
     7261 attacaagta ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa
     7321 tctatgctaa tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt
     7381 cttgtcgcat gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga
     7441 cagatcttga tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact
     7501 catatctaaa gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt
     7561 ctgttaaagt attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt
     7621 ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct
     7681 atatattctt tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac
     7741 gacatttact tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt
     7801 ttacttacaa tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca
     7861 atggtaagtt ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg
     7921 taataattac ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat
     7981 tctttatagt ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat
     8041 aacgagagac gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa
     8101 aatcaatcca tacaatatca ccttttttag gaatataaat tctttcttct gacactataa
     8161 aatttctctc ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc
     8221 ttccccacca ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga
     8281 atacacaacg atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt
     8341 taccactaca gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt
     8401 aataaaagta tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga
     8461 aaattgtttt gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt
     8521 cattttttct ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct
     8581 acccaaatac tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga
     8641 gaaaatttca aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta
     8701 ttccttttgc tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt
     8761 ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc
     8821 aactagacaa gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg
     8881 gattttacat gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt
     8941 cagagaatat acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa
     9001 agagtcataa tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa
     9061 aacaaataga agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca
     9121 gcttatgagc atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt
     9181 tttttgccgc tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag
     9241 gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt
     9301 gttcttcagt caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt
     9361 cagtagtcat tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc
     9421 caatatacac tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact
     9481 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     9541 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     9601 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     9661 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     9721 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     9781 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     9841 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     9901 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     9961 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
    10021 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcta
    10081 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
    10141 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
    10201 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
    10261 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
    10321 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
    10381 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
    10441 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
    10501 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
    10561 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
    10621 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
    10681 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
    10741 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
    10801 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
    10861 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
    10921 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
    10981 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
    11041 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
    11101 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
    11161 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
    11221 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
    11281 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
    11341 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
    11401 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
    11461 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
    11521 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
    11581 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
    11641 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
    11701 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
    11761 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
    11821 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
    11881 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
    11941 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
    12001 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
    12061 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
    12121 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
    12181 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
    12241 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
    12301 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
    12361 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
    12421 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
    12481 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
    12541 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
    12601 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
    12661 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
    12721 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
    12781 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
    12841 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
    12901 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
    12961 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
    13021 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
    13081 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
    13141 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
    13201 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
    13261 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
    13321 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
    13381 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
    13441 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
    13501 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
    13561 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
    13621 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
    13681 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
    13741 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
    13801 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
    13861 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
    13921 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
    13981 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
    14041 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
    14101 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
    14161 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
    14221 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
    14281 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
    14341 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
    14401 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
    14461 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
    14521 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
    14581 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
    14641 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
    14701 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
    14761 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
    14821 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
    14881 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
    14941 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
    15001 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
    15061 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
    15121 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
    15181 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
    15241 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
    15301 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
    15361 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
    15421 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
    15481 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
    15541 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
    15601 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
    15661 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
    15721 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
    15781 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
    15841 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
    15901 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
    15961 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
    16021 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
    16081 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
    16141 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
    16201 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
    16261 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
    16321 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
    16381 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
    16441 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca
    16501 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
    16561 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
    16621 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
    16681 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
    16741 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
    16801 agaatgattt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
    16861 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
    16921 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
    16981 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
    17041 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
    17101 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
    17161 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
    17221 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
    17281 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
    17341 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
    17401 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
    17461 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
    17521 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
    17581 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg tttctttctt
    17641 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
    17701 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
    17761 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
    17821 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
    17881 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
    17941 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
    18001 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
    18061 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
    18121 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
    18181 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
    18241 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
    18301 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
    18361 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
    18421 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
    18481 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    18541 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct
    18601 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    18661 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    18721 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    18781 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    18841 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    18901 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    18961 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    19021 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    19081 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    19141 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    19201 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    19261 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    19321 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    19381 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    19441 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    19501 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    19561 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga
    19621 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    19681 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    19741 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    19801 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    19861 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    19921 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    19981 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    20041 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    20101 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    20161 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    20221 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    20281 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    20341 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    20401 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    20461 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    20521 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    20581 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    20641 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    20701 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    20761 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    20821 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    20881 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    20941 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    21001 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    21061 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    21121 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    21181 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    21241 ataagctgtc ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    21301 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    21361 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    21421 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    21481 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    21541 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    21601 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    21661 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    21721 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    21781 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    21841 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    21901 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    21961 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    22021 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    22081 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    22141 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gcta
//
LOCUS       DACCWV010000038        22136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCWV010000038 DACCWV010000000
VERSION     DACCWV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.038"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..526
                     /locus_tag="IVW84_001230"
     CDS             <1..526
                     /locus_tag="IVW84_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7917436.1"
                     /translation="IGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIV
                     ANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFG
                     SLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTV
                     LLLPLFFQPFAGLF"
     gene            526..2112
                     /locus_tag="IVW84_001231"
     CDS             526..2112
                     /locus_tag="IVW84_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7917437.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2131..2352
                     /locus_tag="IVW84_001232"
     CDS             2131..2352
                     /locus_tag="IVW84_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7917438.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2349..3110
                     /locus_tag="IVW84_001233"
     CDS             2349..3110
                     /locus_tag="IVW84_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7917439.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3490..3999
                     /locus_tag="IVW84_001234"
     CDS             3490..3999
                     /locus_tag="IVW84_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7917440.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4074..4613)
                     /locus_tag="IVW84_001235"
     CDS             complement(4074..4613)
                     /locus_tag="IVW84_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7917441.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4753..5364)
                     /gene="rpsD"
                     /locus_tag="IVW84_001236"
     CDS             complement(4753..5364)
                     /gene="rpsD"
                     /locus_tag="IVW84_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7917442.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5680..6477
                     /locus_tag="IVW84_001237"
     CDS             5680..6477
                     /locus_tag="IVW84_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7917443.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6505..7140
                     /locus_tag="IVW84_001238"
     CDS             6505..7140
                     /locus_tag="IVW84_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7917444.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7160..7933
                     /locus_tag="IVW84_001239"
     CDS             7160..7933
                     /locus_tag="IVW84_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7917445.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8341..9138
                     /locus_tag="IVW84_001240"
     CDS             8341..9138
                     /locus_tag="IVW84_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7917446.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9116..9529
                     /locus_tag="IVW84_001241"
     CDS             9116..9529
                     /locus_tag="IVW84_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917447.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9513..12158
                     /gene="mutS"
                     /locus_tag="IVW84_001242"
     CDS             9513..12158
                     /gene="mutS"
                     /locus_tag="IVW84_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7917448.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12176..14284
                     /gene="mutL"
                     /locus_tag="IVW84_001243"
     CDS             12176..14284
                     /gene="mutL"
                     /locus_tag="IVW84_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7917449.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14306..14866
                     /locus_tag="IVW84_001244"
     CDS             14306..14866
                     /locus_tag="IVW84_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7917450.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14951..16939)
                     /gene="treP"
                     /locus_tag="IVW84_001245"
     CDS             complement(14951..16939)
                     /gene="treP"
                     /locus_tag="IVW84_001245"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7917451.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17162..17875
                     /gene="treR"
                     /locus_tag="IVW84_001246"
     CDS             17162..17875
                     /gene="treR"
                     /locus_tag="IVW84_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7917452.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17952..18398
                     /gene="msrB"
                     /locus_tag="IVW84_001247"
     CDS             17952..18398
                     /gene="msrB"
                     /locus_tag="IVW84_001247"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7917453.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18568..19170
                     /gene="ruvA"
                     /locus_tag="IVW84_001248"
     CDS             18568..19170
                     /gene="ruvA"
                     /locus_tag="IVW84_001248"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7917454.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19183..20184
                     /gene="ruvB"
                     /locus_tag="IVW84_001249"
     CDS             19183..20184
                     /gene="ruvB"
                     /locus_tag="IVW84_001249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7917455.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20213..20791
                     /locus_tag="IVW84_001250"
     CDS             20213..20791
                     /locus_tag="IVW84_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7917456.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20843..21325
                     /locus_tag="IVW84_001251"
     CDS             20843..21325
                     /locus_tag="IVW84_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7917457.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21442..21816
                     /locus_tag="IVW84_001252"
     CDS             21442..21816
                     /locus_tag="IVW84_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917458.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7238 a         3991 c         4790 g         6117 t
ORIGIN      
        1 tatcgggggc aattttttga ctatgctgat tactcctgcg acagtggcac ttgccatccc
       61 tctttataaa aacttccgtt tactgaaaga gaatttttat cctgtaatgg ctgcgatttt
      121 agcaggaata gtcgcaaatt gcttggttag tgtagggttc ggttacttct tctctcttca
      181 taaagagatg gtgatctcat tactacctaa atctgtaacg acggctattt ctgtcgattt
      241 atctcatacg atgggcgggg tgaatgccgt gacacttgct attgttgtct ctacaggtat
      301 ttttggttcg ctgatcgcca ctcatatttt ccgcctattc aagatcgaaa gtccagttgc
      361 tcgcggtgtg gcgttaggaa gtacttctca tgctattggt acagcaaaag caatcgagat
      421 tggtgaaata gaaggaatca tttccggact tgcgatatgc gtcaacggaa tattgaccgt
      481 tttattgcta ccgttattct ttcagccttt tgctggcttg ttttgatgcg aaaaccacta
      541 ttttatgcta caataaccaa aacgttcgag cataaaaaag gagggaaaga cgtgtatttg
      601 tcttccgagt atcttaaaca atttgataaa gatctatatt ataagattct cctcctagaa
      661 agttttgagg atcaagcttg gcataccgct gcacaattag cgcaagttgt tcagctggat
      721 gcccgcagcg tgtcgaaata cctgaacgag ctgtctaaaa actatcagca gtttagcggt
      781 aagacacacc ctttgtttac aaaaaatcac cgttctggtt ataactttta tgatacgctc
      841 gattcaattg aacatgagcg ttttttgatc tatctcgtgc aaagtacgct gaaattccag
      901 ttgctgcacg acattttttt tgaagaattc catacaatgt accaatttgc gcaaaagcat
      961 tacattagtg aatctactgc tcatcgaaaa atcaatgaat ggaaacagca actgcaaact
     1021 tacggaattc ggctgcaacg aggtacatat atcgctcaag gcgaagaaga aatcattcga
     1081 ctttacttgc atatgacatt ctggcagttg tttcgaggga aaatctggcc atttgaaacg
     1141 atttctcaaa tggacgtaaa gaatatggca gaacatatca tggctttttt taatgttcgt
     1201 ctgaacgaga tcaaaaaaag acgattggaa tatatgctag gtgccttttt tttaagaaaa
     1261 tcacagaagc attatgttgt attgaatgag aaaaaaagac ggctgatttc tgacaatctg
     1321 ttgttccagc gattttgcca ggtcatggag ccggtatttc ctaattattt tcaagtagaa
     1381 gacgagttag gcgcattatt tcttgtactg atgactcgtg aagaatatta tagtgatcct
     1441 aagatacgga agaaaatttt tgattttcat caacaagcaa aaacaccgcc ctttacagcc
     1501 ctttcagaag caaaagccgc tctttctctt tatcaggaag agcaggggct gccagcagaa
     1561 aatctaacct tcgaggcaga aaactattta ttcagcagtc attttttcgc ctatcttttt
     1621 cctaatgcga aagaaacgat tgatggcaat agtagtgatt ttattaatca tttagttatt
     1681 gaaaacaagg aattgaaaca atggctcgta catttttttg aaagtagaca caaacatccc
     1741 aatcatctgg catttaaaaa tcatgcgttt ttgatggggc ggtatctgac tgtgtttaaa
     1801 acattaggag cctttactcc acagcttcca aagatcacga tccttttaat gacagatttc
     1861 ccattatttg aagagcaact gttagaggag ggattacgga catttttccg taatgagtat
     1921 cagctgattt ttctgccaac agattatcgg gggagagaag ttgatcttct gatttcgaca
     1981 agtaaagtcc accgcaaacc atgggcggat cttgactatt ttattgtgac tgaagaacta
     2041 aaattaattg attacatcca actatctcaa aaatttgaaa tgattcaaaa acaaaaacta
     2101 ttaaaacaat aacaaagaag gagaaaaaag atgacatttg aagaaatttt accagaattg
     2161 aaaaaaggtg caaagattat tcgtaaagga tggagtggat ttgaacttta tgttgttctc
     2221 gtatccgatg atgaatttga tggctgtccg gtgacgccgt acttattgat caaaacaagt
     2281 gacgaaggtt tttccagctt cgctcctaca gtttgtgata ttttagcaga tgattgggcg
     2341 atcgtagaat gaaaaaccag tatccagatc tagctaaaca ggttgtattt atcacaggag
     2401 ctgcttctgg aattggtgct gcgcaagccc gagcgtttct tgaacaagat gcttttgtat
     2461 ttggattaga tcaaaagttc ggtaaaatgg aagaaatttc tttgcgattt cctgaaactt
     2521 ttgcctacgc actaggtgat gttcgacaaa tgaaagaact ccaacaagcg gtatcgaaat
     2581 gccaaaatca ttttggtgaa gttacgattt tactgaatac agcggggata ttggatgcct
     2641 acaaaccctt gatggaaact gacgaatcgc tgtgggatct gatttatgag acgaatgtga
     2701 aaagcatgta tcagctgaca aaaatcatcc ttcctgatat gatccgccaa aaaagcggaa
     2761 cgatcatcaa tatggcttcg attgctggct tgattgctgg tggaggtggg atcgcttata
     2821 caagcgcgaa acatgcgatt gtcggattta ccaaacaact cgctctggat tacgcaggac
     2881 agggtatccg agtaaaagga attgctccag gagccatcca aacaccaatg aatgcggctg
     2941 attttgaagg gaaaggtgag atggcagagt gggtggcaaa tgaaacacca gtcaagcgat
     3001 gggcacagcc ggaagaagta gctgagctga cgttatttct tgcctctcct caagcaagct
     3061 acatacaagg aacgatcatc ccaatcgacg gcggatggtt gttaaaataa ccttgtgttt
     3121 ttttcaagct tctgtatgat aaaactaaag agagaaacaa cggttgatga taaaaaaagt
     3181 cgatgactgt tcccgacatt cgatgccgaa cagccagttc aaaaaattct ttttaataat
     3241 cgttaatatt cgaaactaag cggcttttca cgacctcttg atataaacga tattcaaaag
     3301 cttgcaactg cggtaacaag tccagccaaa tgaaaaatat aaggagctat ttttatttga
     3361 ccattcttat tacttgttgc agggcagctt tttcactacc ttctgcgaca cttcagttgg
     3421 aattttcttc ccatcctttg gtcgcggtca ttaccaagat aaaattatct tgcaaaggag
     3481 aaatagaaca tggaaaaaaa taatacccgt tcagccaaac gctggacaac gacagatgtg
     3541 actcggatgg cgctagtcac tggtttatat gtggcagtga cgctggtttt gtctgtcttt
     3601 agctttggag tgattcagat tcgtctctcg gagatgttca actatctgtc cttgtataac
     3661 aaaagataca tcgtcgcggt aacagcggga gtagctttgg ctaatattgc ttctcctctt
     3721 ggactgatag atgttgcagt aggaagcgtc agcacgctgc ttgtattgct tatcaactat
     3781 aaaatcacta gtcgcatcaa aaacatgaaa atcaaaatgg ccgtgacagc atgcgtattt
     3841 gctttttcca tgtttacagt agctggtcag ctaacgattt tatatcaact accttttttc
     3901 ttgaattggt gggtgattgc tttaggagaa ttggcatcaa tgattgtcgg tgggataatt
     3961 atttattgga tcggtaaaaa agtggatttg tcaaaataaa gatctgttta atttaaaata
     4021 gggcagcatg gaagagactg aatgttgagt cttcttccat gctgcttttt tgattaattt
     4081 gtatgaagaa atcgatttag ccgttttttc atcaagatac cgatataagc tgctccagtt
     4141 gccttgattg cttccggcag taaaaacggc agtagtccac tggcaaatgc agttgtaaaa
     4201 gtaagatcac cacttatttt cagccagatc gttccgcaga tcaatgcagc aagagagccc
     4261 attagattgg caaagattgc atataagtaa tgagcatccg ttctttcgat catccaacca
     4321 gttatccacg tttgtaagag aaatccaatc aaatatccgc cagttggccc aaataaaaca
     4381 gctatacctc cagtcatccc tgaaaataca ggaagaccaa tcaagccaag taaaagataa
     4441 actccaacag atatcaatcc tagttttctt ccgagaaaag ttgctgttaa tccaatagca
     4501 aatgtttgcc ctgtcaaagg aaccaaacca aaaggaatgg tcatctgaga aaggacagaa
     4561 ataatcgcgg caaattccgc agcaagtatc aattctttca atgttcggtt cataacgccc
     4621 tcttttcgtt aacttaatat ttttatttgg ttaacgaaat tgtaaaacaa agtgattact
     4681 ttgtaaatag aaaatataaa aaaagaccag ctatccaata gatagcggtc tttttcaaga
     4741 aagcttttga aattataagc tttggttgta gtattcaacg acaagagctt cgtcgatttc
     4801 tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt tttcagcgtc
     4861 aaagcttacg aatgctgggc gtccaacagt tgcttctaca gcttctttta ctgttacgat
     4921 gttttgtgat ttttcacgaa ctgagatcac ttgacccact tctacgtggt atgaagggat
     4981 atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta actaattgac gtgcttgacg
     5041 acgagtcgtt gcaagtccta aacggtaaac aacattgtct aaacgttgtt ctagtaagat
     5101 catgaagtta acaccatgtt taccttcttt gattttgcta gcttttacga acaagttacg
     5161 gaattgacgt tcgttcattc cgtacatgtg acgaagtttt tgtttttcag ttaattgcat
     5221 gccgtattct gattgtttgc cacggctatt aggtccatgt tgacctggtg cgtaagggcg
     5281 acgtgctaat tcttttcctg tgcctgacaa tgagatgcct aagcgacgag aaattttcca
     5341 tgatggtcct gtataacgag acattaaaat ttcctccaat gaatataagt ttttggagta
     5401 aaataatccg ttgaaaaatt cataatcata tagtttgttc ttcaatcttc acctttgcag
     5461 ccgcggttac acaatcgaac ccgagacggg gcaacaaact agggatgagt tattattttt
     5521 ctgctgcatt attttacaca aattacagta tatgatagat catccacgaa tgtcaagata
     5581 attagtacat aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag agggtatgtg
     5641 caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt atcaccatta
     5701 tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa cagcttttca
     5761 cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac ggctgtggca
     5821 tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc taggaaagat tttatacgca
     5881 tttatgttca gctggggtct ggtcatgatt ctatacatga acgccgaatt aggaacatcg
     5941 aatatgcttt atatgaccgt gggtgttttt cggcagagac ttgctttaag taaggcagcc
     6001 aagattctat ttgcctgtat ttttttcaat atgattggcg gtatattatg tggatttcta
     6061 atttctatga ctacgccatt tcaagattta acgttggaca actttatgtt agaatcaata
     6121 gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg aagggatgtt tgcgaatatc
     6181 gttgtcaata ccgctgtact gatcagcatg cggatgaaag aggatgtcgg aaaagtgatc
     6241 acagtcgtat tcatcatttt catttttgct tttttaggat atgaacacgt gatagcgaat
     6301 tttcctgcct ttacgcttac ttactttgct tctcacggac agatggatgg aatgacagtc
     6361 agcaatgtgt tacataattt gttttttgct ttggcaggaa attatattgg tggcggttta
     6421 gtaatgggac tgggatatgc ttggctgaat cagtcaaaat catcctatgt ggactaaaaa
     6481 gtcgttcatt aaaggggaag tgccatggaa gttttagttt ttccaaaaaa tgaggaaaaa
     6541 gcgttacaaa tggaaaagta catgaaaaat cttttttcct ttgctggagt acctaagccg
     6601 gaacgcgctc ttttacaaca accgttactg gatgagagca aaaatttatc tatttctgaa
     6661 gttttagctt ttgttcatta ttattatgaa caaatcgaaa gagagtatca gtatgtagcg
     6721 atcgatttag tccagaaaaa catcaaacgg cttacatttg aagacttagt cgtattgttg
     6781 ccactagttc agaaaaaaga atggtgggac agcatcgact cttggagaaa agtatacagc
     6841 acatggtgta aagcctaccc agagcagcgt gaagaagtct ttcaattgtt tgaaaaaaag
     6901 gaagattttt ggctacgtcg aatagcaatc acgttgcaat taggttataa acagcaaaca
     6961 gacacaggac tattgatccg agcaatagac gaagatcgtc aaacaaagga attctttatc
     7021 caaaaagcaa ttggttgggc attacgcgat tatagtaaga ccgatgccga ttgggtaaaa
     7081 agacagctga caacagattt gagtaagtta gctgttcgag aggcaagtaa gtatttataa
     7141 gaaagcgaga cgacaagaga tgagacgcaa attatttgct tttgatattg atggaacgtt
     7201 gttagattca aacaaacaag ctttagatag cacaagagaa gcattggaaa aattacgcaa
     7261 agccggtcat ctggtcacga ttgctacagg gcggagccga tttcatgcgc aagagatcat
     7321 tcgcgatctt gattttacga actacatttt atgtaatgga gcggccggtt ttttagacca
     7381 tgaacaagta tataagaatt tactagatcg cgaccagttg gatgcgttcg ttcaagaagc
     7441 gcatcatgag cagatcgata cagcttttgt cagcttagac aatatcaagc gttcttcttc
     7501 caatgatgta acggtgatgg aagaagcgat gcattctttc ggtgctgtct tgcctgaatt
     7561 agatcagttt tttgcagagg aacaagaaat ataccaagcg ttagcatttt tcgatcaaac
     7621 atatgatggg aagttctctt catatgataa aattcgattt gtccgctggc atgagaacag
     7681 tgtagatgtc gtacctaaaa atggttctaa agcagcgaca attctgcact tagcagaaca
     7741 agtaggcatc cctcatgaag acatcatcag ctttggcgat ggacaaaacg accgtgaaat
     7801 gttgcgcatg tcaggaattg ggatagctat ggggaatgct gtgccagaag tacaagcaga
     7861 agctaagatg gtcactgata caaacgatca ggatggaatt tggaaggcat tgaaagagtt
     7921 aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agcccagcca
     7981 aacaaaaata gctcctccta tttttcgttt ttctgggctt attaccgcag atgcaagctt
     8041 tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc agtttcaaga aataagaagg
     8101 gatttacgaa aattgcttct caaatttttg taaaattacg gcttatttct gaagaagtag
     8161 atgaaacaac gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat agtcccattt
     8221 ttttatttta tttttcaaaa ctaagaaata ccaggaaaga gttccttttg tttgaccttt
     8281 tctttcttat tgaaaacaac tataatatag agtgatgtta aaagagaaga ggaatttagt
     8341 gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg gacgagagat gattactgaa
     8401 tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa tggtgagaat
     8461 gccgcttctg gacgtggaat cacagaaaaa atctataaaa aatttttgca agacggcgta
     8521 gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg gaatctttga gtttgtaaat
     8581 gaagctaaaa agatggttcg tccggcaaat tttccggaag ggacgcctgg acaagggatg
     8641 gtctttgtca aagtgaacca agtcgaactg gcagtgatca acatgcaggc tcgttctttt
     8701 atggtggact tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga agccagaaaa
     8761 cgtacgccga tcatttttgt agattttcat ggagaaacaa caagtgaaaa acaagcaatg
     8821 ggctggtttt tagatgggaa agtttcagca gtggtcggta cacatacaca cgttcagaca
     8881 aatgatgcgc gaattttgcc aagaggaaca gcgtatctga cagacgttgg gatgacaggt
     8941 ccctatgatg gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt tctgactgca
     9001 ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg ctgtatcttg
     9061 gaattggatg atacgacagg tcatgcaaaa gaaatccagc tgattcagat caacgatgat
     9121 cgaccgttca tggagtagaa agagaggcag ttttttgcag caggagccgg aaattcaaaa
     9181 agaagtaagg aaactgacaa aacttttaag agagaatgaa acgatcattc gctacaaaga
     9241 acttgaagaa aagatccagc aaaaccagta tttagctgag ttgagagaaa aaatcaaaca
     9301 agcgcaaaaa gatgctgttc attttgccca ctatgacaaa ccagcagcag aaaaagaagc
     9361 aatcaaacaa gcggatcaat tcatgcaaga atttgatcag catccgcttg ttgtagctta
     9421 tagaaaacag ctgttggaag cagatgatct gcttcatcat ttaacaacta tgatacaaga
     9481 agaaatcaat ggacaaatag aggaggaaaa acatgcctca aaaaactaaa aatactccta
     9541 tgatggaaca atatctaagc atcaaggccc aataccaaga tgcttttttg ttctatcgtt
     9601 taggagactt ttatgaacta ttttacgaag atgcgatcaa agcgtcacag cttttggaat
     9661 taacgttgac gagcagaaat cgaaatgcag aagaacctat tcctatgtgc ggtgtacctc
     9721 accatgcagc acaaggatat atcgatgcct tgattgaaaa aggctataaa gtagcgattt
     9781 gcgagcaggt agaagatcca aagacgacta aagggatggt caagcgcgaa gtgatccagc
     9841 tagtcactcc tggtacagtc atggacagca aaggattgtc ggcaaaagac aacaattttt
     9901 taacagctgt agttagtcaa ggaaatgaat ttggctttgc ttatgccgat ttaagtacgg
     9961 gagaattgaa gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa gcctctgcat
    10021 tgcagacaaa agaactcgta ttaggcagtg agatcactga tacccttcgt gaacagctaa
    10081 gcgggcgttt agggctagtc ttttctcaac aaaatgagat ggaagaaaac gcggaattta
    10141 gctttttgac aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc aaactattga
    10201 cttatttatc ggtgacacaa aagcggagtt tagctcatat ccaaaaagca gtagaatacc
    10261 aaccagatca ttttttgaaa atggattatt attcaaaatt caacctagaa ttaagccaat
    10321 ctattcggac tgggaaaaag cacggcactc ttctatggtt gttagatgaa acaaaaacag
    10381 caatgggtgg ccgtttacta aaacaatggc tagaccgtcc gcttatccag gcaaaacaaa
    10441 ttaaagcaag acaagaaatg gtcgcttctt tgctagacag ctattttgaa cgtatcgatt
    10501 tgcagtcagc attaacgaaa gtctatgatc tggaacgcct agcaggtcga gtagctttcg
    10561 gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc tttagaacaa gtgccaatga
    10621 tcagagggtt gattgaagga atcgatcaag gtcaatggca aagtctgctt tcagaaatgc
    10681 agccgatgga ccatctcgtc caattgatcg accaagctat ccaagatgat ccgccgttac
    10741 aaataacgga aggaaacatc atcaaagatg gattcaatga gcagctcgat atgtaccgtt
    10801 ctgctatgag aaacggaaag aaatggttgg cagaacttga agcaaaagag cgtcaagaaa
    10861 caggaatcaa gaacttaaaa atcggattta accgcgtttt cggttattat atagaaatta
    10921 caaaagcaaa tcttggaaat gcagaactgg agaagtatga acgaaaacaa actttagcaa
    10981 atgcagagcg tttcatcaca ccagaactaa aagaattgga gacacaaatc ctcgaagcag
    11041 aagaaaaatc tgttgattta gaatatcagt tgtttttagc tgtgagagaa gaagtcaaaa
    11101 aggctatcca gccgctacaa gtacttgcaa aagcaatcag tgcagtagat gtgctacaaa
    11161 gttttgcaac gatcagcgaa cgctatcagt atgttcggcc agaacttgtc agtgacaagc
    11221 atcagcttct gatcaaagac ggacgacatc ctgtagtaga aaaagtatta ggacatcagg
    11281 aatatatccc taacagcgta gaaatggcag aagacgaaat gatcttgctg attacaggac
    11341 cgaatatgtc tggtaaaagt acgtatatgc gacagttggc attaactgtt ctgatggcgc
    11401 aaatgggctg tttcgttccg gcaaaacaag cagttttacc tatttttgat cggattttta
    11461 cccgaattgg tgcaagtgac gacttaattg ctggacaaag tacttttatg gtagagatga
    11521 tggaagccaa tcaagccctg cgttatgcta cgccaaacag tttgattttg tttgacgaac
    11581 tagggcgagg aacagcaacc tatgacggaa tggcgttggc ccaagcaatc atcgaatata
    11641 tccatcgtca tgtacaagca aaaacactct tttctactca ttatcatgaa ctgacggtac
    11701 ttgagaaaga acttccgcag cttaaaaatg tccatgttgg agccgttgaa aaagacggag
    11761 aagttgtctt cttgcataaa atgatggatg gacctgcgga taaaagctac ggcatacatg
    11821 tggcaaagat cgcaggattg ccttccaatc tactagaacg agcagcagat atcctgcata
    11881 cgctagaaaa tggcgataac ggacaccata ctgttccagc tgaagtagaa gagaaaacta
    11941 aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa gaccatatac
    12001 aagaccgtgt aaaagaagaa aaccaacagc tttctttatt cagtgaatta tccgaaaatg
    12061 aaacagaagt cattgaatca ctgaagcaaa taaatttact agaaatgacg ccaatggatg
    12121 cactaaacac attatatgaa ttacaaaaac aactgtagga agaaggcgga tatcgatggc
    12181 caaaatccaa gaattatctg aacgtttagc taatcagatc gctgcgggag aagtagtgga
    12241 gcgaccagct tccgttgtga aagaattagt ggaaaatgca attgacgccg gcagtactca
    12301 gattgatatc ttagttgaag aagcgggact gcggacgatc caagtgattg ataacggaga
    12361 aggcattttg gcagacgacg tagagaatgc cttcaagcgg catgcgacaa gtaagatcca
    12421 taatcgcgac gatctttttc gcatacgaac gctgggcttt cgaggggaag ctttgccaag
    12481 tattgcctct gtttctgaaa tgactctgga aacggcgaat caggaagaaa aacaaggcac
    12541 ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga ccagcaccac tgcgtcaagg
    12601 aacgaagatt accgtcagca atctgttttt caatacgcca gcccgcttga aatacgtgaa
    12661 aacactgcac acagaattag cgaacatcgg ggacatcgtc aatcgactgg cattgagtca
    12721 tccatcgatt gcttttcgtt tagtacatga tggaaacaaa atgctggcaa ctgctggaaa
    12781 tggcgatctg aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa aaaaaatgct
    12841 gaaaatcgaa ggaaaagatc tagattttga gttatttggc tatgtttcat tgcctgaggt
    12901 gactcgtgcc agtaggaatt acttatcgac gatcatcaat ggacgtttta tcaagaactt
    12961 ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg gtcgtttccc
    13021 tattgctgta ttggaaatca aaatggaccc gcttttagta gatgtaaatg ttcatccgac
    13081 gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca gagctaatta gtaacgccat
    13141 tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca gcggataatc tacggtttaa
    13201 gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa atcgatttgt cctacgcttt
    13261 tgaagaagaa aaacctaaaa agccaagtag tttgaatttt gatcgacaga caggtacctt
    13321 tttcgtagaa gaactaaaga acacatttgt tgacagaatt gttgataagt ctgtggaaaa
    13381 tgttgaaaac tattctggtt tttctgaaga aaacaccact tatccagaaa aaacagcgaa
    13441 aacggtggat aagtttggtg aaaatgttga taactttgta gaaaaaacag tggaaaaatc
    13501 tgaaaagaaa gaatttccca ttgtggataa catggagaaa acaaatgaag aacaagcaga
    13561 taaatccgta gaagaggatg aaaagattta cgaggaagaa ttgacactcc atcctgaatt
    13621 tgatttcact caaggaaacg acaaaacatt agaacaagcc atcaagaaat tagaaacgga
    13681 aaaaccagta caacgttttc ctcagttgga atattttgga cagatgcacg gaacgtatct
    13741 ttttgcccaa agtagcgatg ggttgtacat cattgatcag catgctgccc aagagcgcat
    13801 taagtatgaa tatttccggg aaaaaattgg ggaagtatct gatgatctgc aagaattgct
    13861 tgttccattt gtgttagact atccaaacag tgatgctttg aaattaaaag agcaaaaaca
    13921 cttgctagaa gatgcaggga tttatttaga agaatttggt caaaacagct ttattgtacg
    13981 ggcccatcct acttggtatc ccaatggaga agaagaatcg attattcggg agatgatcga
    14041 tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa cagcaatcat
    14101 gatgagttgc aaacgatcga ttaaagccaa ccattatttg aatgaagcac aagcacgtgt
    14161 gctgctgaag gatctggcac gatgcgaaaa tcctttcaat tgtccccatg gccgtccagt
    14221 attgattcac tttaccaatt cggatatgga aaggatgttc aaacgaatcc aagacccgca
    14281 ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta gcatcccaat caccaagaag
    14341 aaaagagtta ttggctcata tcgtttcaga attcgagatt gaacctgctg atatcgatga
    14401 aacgccattt ccagacgaac agcccgtcga ttatgttcgt agaatggctg aagaaaaagc
    14461 acgaactgtc tgggaacaag atgaaaaaga agaccattta gtgattgcca gtgacacgac
    14521 tgttgttctc aatcaagaga tcatgggtaa accagaagat ctggctgagg ctgaattcat
    14581 gctgaaaaaa ctaagcgggg aaacacatac tgtatataca gctgttgttt taaaaacaaa
    14641 ggaacgagaa gaacggatac tagcagaagc acatgtcact ttttatcctt tgacagacga
    14701 agagatcaaa cgctacttgg agacggggga ttatgcggat aaggcagggg catacggcat
    14761 ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga gattattaca gtatcgtagg
    14821 atttccgatt ggtgctgtca atcaggcact gaaaacattc atttaaacaa aaaagcggac
    14881 tatctgattt tcgattgaaa atccatgata gcccgttttt tttattgaaa agattactta
    14941 tctactctga ctaagatttt acttcagctt taccgactgc ttcgcccggg cgaacaagtc
    15001 ctgattggtc aagagaaagt cctgcaactt tttcattatt ggtcaaagca acaataatgt
    15061 cagttttctt gccagcttgt tctatttctg gcaaatccat ttcagcaatc agggtctctg
    15121 gtgtgacaag gtatccttct ttcacgaata cattgaaagg aacacccttt agttcaacag
    15181 tatctaatcc catgtgaaca agtacttcca gcccctcatt agaaagaata ccaatggcat
    15241 gttttgtttc aaagacactc gtcactttac cgtttactgg tgcatagact ttgccgtttg
    15301 acggttcgac agcataaccg tcacccatca ttttttgtga gaaaactggg tcatttactt
    15361 cagttatttc cttgatggtc cctgcagcga ctgcgaataa tgtttctttt gttgcaactg
    15421 ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg ccatttgctg
    15481 tttgaagttg tacatcagca gcattttcag gaacagcagg atcaatctta ttcaaaattc
    15541 cctgacgtct aaagatgatc gtcaagccaa atggaatgat gacagcaatg atcattgcga
    15601 tgatgaaagg tacccatgtt tctgcttgga tagctagtat tcctggcaaa ccgcctactc
    15661 cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat gccagctaat ccagagccga
    15721 ccattgcagc gacgaaagga tagacgtatt tcaaattgat tccgaacata gcaggttcag
    15781 ttacgcctaa gtaacaagaa atcattgctg gaatcgagat ttgttcttct tttttattcc
    15841 cacgatgtaa gaagatgatg gctaagactg cagagccttg ggcgatattt gataaagcaa
    15901 tcatcggcca taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg atggcattgg
    15961 tcatatggtg gagaccagta atcactaaag gagcatacag gaatccgaat acggcactaa
    16021 acaaccagct gatagaagac gtcaatccag tatttacgat atttgagatc cagcttccga
    16081 ctgtccagcc gataggacct aaaataacgt gtgcagctaa aacagttggt aacaatgaaa
    16141 acaatggaac aaaaatcatc gagatcgact gagggatata tttgcggaag aagatttcta
    16201 gatacgctaa cataaaccct gccagcatgg ctggaataac ttgcgcttga taaccaatca
    16261 tctggacttg agcaaaacca aaatcccaaa atggaatatc tgctgcggct gtggaagcta
    16321 cactataagc atttaataat tgcggagaaa ctaacgtaat tcctaaaacg attcctaata
    16381 tttgtgtagt acccatcttt tttgcaatac tccaagtgat acctactgga agaaaatgga
    16441 aaattgcttc accgggtaac cacaaaaagg cattgacacc attccaaaac ttcgagtgtt
    16501 caacgattgt tccaccaaga ctttcaaact ggataccttc aagtacattt ctgaatccaa
    16561 ggatcaatcc accgacaatg attgcaggaa tcagtggtgt aaagatttct gctaaaacgg
    16621 caatagcacg ttgaacggga tgaaggtttt gttttgcagc tgcttttcct tgttctttgg
    16681 aaaccccttc aactccagat actgcaacga agtcgttata aaaagtagaa acatcgtttc
    16741 ctatgatgac ttggaactgt cctgcattcg tgaacatccc ttttacgcta ggtatatctt
    16801 cgatcgcttt ttcgtccgct tttcctggat cattcaggac aaaacgcata cgagtcgcac
    16861 aatgactaac tgcagcaata ttttcttttc cgccgatatc atgtaacagc ttctcggcat
    16921 ctgcttgata ttttgccata tcaataaacc cctttctctt aacttgtcta tgcaagttgt
    16981 ttcttcattt actatacttt ttttcggaga gaattgcaag cgttttatta aaaagtacga
    17041 gagaaaatga tatcgtttta aaaagcttaa aataagcttt tcttaaaaat aggaacattg
    17101 aaatacaaaa agagctgtag tatagtgaag aaatgatgga agaacgagtg agggaaaaaa
    17161 catgaataag ttcaatgaaa tttttttaga tttggaacag aaaattttag cgaaagaata
    17221 tcctccgcat tcgcttttac ccagtgagaa ccaattgatc aaaatttatg gtgtttcacg
    17281 agaaacaatt agaaaagcgc tgaatctttt gactacggaa ggttatattc aaaaaaaaca
    17341 aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac tttcctattt ctggattgac
    17401 tagctataaa gagctacagg agacccaacg tattccaagt gaaacgatca tccatacgtt
    17461 agaagaaaca gaagtgacaa aagcattgaa cgagatcact ggctgggaga tcggtgcgcc
    17521 tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa gtagttatat tagacaccga
    17581 ttatctatta aaggaaatcg ttcctatttt aactccagca caagcacaag gatcgatcta
    17641 tgaatatttt gaaaatgagc tttctttgac gattgcttac gcgcaaaagg agatcacagt
    17701 agaagaagta acggaaatca tgaaaacagc gatggatctt cacgatgaca cttatgcagt
    17761 agtagtcaga agcctcgttc atctggaaga tacccgctgt ttcgagtatt ctgagtcggt
    17821 tcaccgatta gataaattcc gtttcgttga gtttgcccga cgccgaaaag tgtaaatagt
    17881 tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg ctatgatagg atcacaaagg
    17941 gggcattttt aatggataaa ccaataagac cagaaaaaga agaactgaaa gaaaagttat
    18001 cacccatggc ttatgcagtc acacaagaaa atgcaacaga acgaccattt agcgggaaat
    18061 atgatgattt ttacgaaaag gggatctatg tagatatcgt cagcggtgaa ccgttattca
    18121 gctctgcaga aaaatatgat gcaggttgtg gctggccatc attcagcaaa ccaataacga
    18181 aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata gaagttcgaa
    18241 gcaaagaagc agattctcat ctcggacatg tgtttaccga tggaccagta gatcaaggtg
    18301 gtctacgtta ctgcatcaat tctgcctcac tgaaattcat tccatatgaa aagatggatg
    18361 aattaggtta tggggaattc aaatcacttg tagaataatg gaaatacaca aagagcggta
    18421 caatacgggg atttactcga tcgtccgctc tttttttatt caagcgattg ctatttgaaa
    18481 gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt gctacaataa agggaacgta
    18541 tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc tgacaggtgt ggttaccttt
    18601 atcaacccct attacatagt agtagaaacc aatggtatcg gctaccagat cgcactagga
    18661 aatccttacc gctacagtag caaactaaat caaaaaataa aaatttatgt ccatcaggta
    18721 atacgtgaag atgcgcatct tctgtatgga tttgattcgc tagaagaaaa gcaactgttt
    18781 ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg cccttgcaat catggcaagc
    18841 gaagaccact ccggtttgat tcaagcagta gaatccagtg atgtcactta tttgacgaaa
    18901 tttccaggag tagggaaaaa gacagcacaa caaatgatct tggatctaaa aggaaaattc
    18961 ggtgagctga gtatcgatac accttttagt ctatttgatg aagcaaacac aaaagacgct
    19021 acagctctat ctgaagcaat ggaagctctc tcggctcttg gttacagtga tcgagaaatc
    19081 aaacgtgtag aaaaacaatt aaaagaagta gataatcaga caacggatga gtatctacgc
    19141 caagcactca agctaatgat gaaaaaataa ggaggagacc aaatgacaga tgaatacttg
    19201 ttatcaccag aatctggcga aaatgaactg agtttggaaa aatctcttcg accacagctg
    19261 ctttctcaat atatcggaca gagaaaagtc aaacaagaac ttcagattta catcgaagct
    19321 gccagaaatc gggaagaacc tttagatcat gttctgttgt acgggcctcc tggattagga
    19381 aaaacgacga tggccatggt cattgctaat gagatgaccg taaatattcg tacgacgagt
    19441 ggccctgcta tcgaacgtcc gggtgactta gtagcaatct tgaatgaact ggaagcaggg
    19501 gatgtcctgt ttatcgatga gatccatcga ttacctagag tggtagaaga gatgctttat
    19561 tcagctatgg aagatttcta tgtagatatc atggtaggac aagggccaac cgcacatccg
    19621 gttcatttcc ctcttcctcc ctttactttg attggcgcga cgacacgggc aggtatgctt
    19681 tctgcacctt tacgagatcg atttggcatc atttctcata tggaatatta tgacgaacag
    19741 gatctgcagg agatcgtttt acgttcttcg gatatttttc aaacagagat cattgaagaa
    19801 ggggcattgg aaattgctcg ccgatcacga ggaacgccta gaatcgctaa tcgcttgctc
    19861 aaacgggttc gtgattttgc gcaagtccaa ggagacggga aaatcgatcg tcctatagcg
    19921 gatcaggcac ttactttgct acaggtcgat caggctggat tggattatgt tgaccagaaa
    19981 ttgttgcgaa caatgatcga gttatatggc ggcggaccag tagggttaag tacgctttca
    20041 gtaaacatag gagaagagcg tgagacggta gaagatatgt atgaaccgta tctgatccaa
    20101 aaaggatttt taaagcgtac acctagaggg cgaattgcta ctgcttatgc ctatgaacat
    20161 tttggttacg actatcatat atgatttttg cttttagatt ggaggaaaat gaatgagtga
    20221 tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt gatttgtgcc aaacgcaatc
    20281 ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca aaaatcaatc gacaaacatt
    20341 ttattatcat tttacagata aatacgattt gcttcgttgg gtctattgtg aggatgcatt
    20401 gcattattta gatccacaat gtctttcact tgataattgg gaagagcaag cattgaaaat
    20461 gctgaaagca attgctgaga agaaagaatt ttatgcaaat actgtgcttt ctgacccaga
    20521 aattttacaa aaagagtttt ctcaactgat ccaaaaatca tttatcaaga tttttgaaca
    20581 aatggatgaa gaaagacaac tttctgcaag agacaaacta ttttatgctc gttttttttc
    20641 ttacggttgc agtggtgtat tagtcaattg gatcacgaat gaatatgaag aaacaccgat
    20701 ggaaatcgct atgcagcttt tccgtttagc caaggatacg gaattatatt cttatcgtct
    20761 ttatgcaaaa gaagagttgg aagaacagta atagaaaaat tttttgttgg aaaagcaata
    20821 atcgctaaaa ggagatgggg aaatgacaaa aattttattt gtttgtttgg gaaatatttg
    20881 ccgttcaccg atggcagaag gcttgctgag aaaacgaata gcattagaag gaagggaagc
    20941 agaatttttt gtggattctg ctgctacaag cacgtatgaa gttggaaaga caccgcaccc
    21001 tggaacgaaa aaaatattaa accaagaaaa agtagatatg acgaatatgg ttgctcggca
    21061 aatcacaccc cacgattttg aaacttttga ttggatcatt ggcatggatc aagaaaatgt
    21121 agaagaactg aaacgaagag caccacgctc agcgcagggg aaaatccatt tgtttttaag
    21181 tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca tactatacca ataactttga
    21241 tgagacatat cacttgatca cagaaggcct gaatcattgg tggaatattt ggattgatga
    21301 aaataaaata acaaacaatt tgtaaagtta acgctttctt tttaaggcga tgttagagca
    21361 tttccttgtt tttttgtaaa atgttaggta aaataaaatt aattaagtaa cactttctta
    21421 gctaaaggag gagtgcattc gatgaacgaa gagacagtat cgccaatgaa gaagcttacg
    21481 aagttagtta ctcatacttc gtatcaactt tttgattctt ttgtaaatga cactgaagaa
    21541 catgctaaga atgtacgatt gcaaggaaag tttattcttg agctgcaaaa agctgctact
    21601 gaacatagtc tagttatctt acaagtaaga gcaaatcgtt tgagtagcaa gtatgaaacg
    21661 atctcaggct gggtggcgac aaaaacggtc gaaaaagacc gagtgatgat ccgcctacaa
    21721 aacgaagagc agcagatccg gatggttcct gtagaacaaa ttgaaaaaat aacaatcctt
    21781 tcaccagcag gggaacagga acggttatca aaataattta taccagatag aaaagcacgt
    21841 atgagcttgt accatcaatc attactaatc aaaaataacc gaatgcttta agctgatatc
    21901 agtaaaacgg aacgttttag gatgagtatc atagcatgac agcattcggt tgattagttg
    21961 aatcgattgg atggtacaag tttttttgat aaaaaaaggt cctgctagcc ggggagctag
    22021 caggataagg agttaaaaat gaaaaagtgt tgttagggtt gtttgttggt atgcttatat
    22081 aatagtcatt agttgtgaat attttgtgac gaaagttttc gaaagttgtt aagttt
//
LOCUS       DACCWV010000039        21738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCWV010000039 DACCWV010000000
VERSION     DACCWV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.039"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(188..1087)
                     /locus_tag="IVW84_001253"
     CDS             complement(188..1087)
                     /locus_tag="IVW84_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7917459.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1097..1453)
                     /locus_tag="IVW84_001254"
     CDS             complement(1097..1453)
                     /locus_tag="IVW84_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7917460.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1808..2104
                     /locus_tag="IVW84_001255"
     CDS             1808..2104
                     /locus_tag="IVW84_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7917461.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2748..3701)
                     /locus_tag="IVW84_001256"
     CDS             complement(2748..3701)
                     /locus_tag="IVW84_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7917462.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3724..4479)
                     /locus_tag="IVW84_001257"
     CDS             complement(3724..4479)
                     /locus_tag="IVW84_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917463.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4476..5435)
                     /locus_tag="IVW84_001258"
     CDS             complement(4476..5435)
                     /locus_tag="IVW84_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7917464.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5428..6378)
                     /locus_tag="IVW84_001259"
     CDS             complement(5428..6378)
                     /locus_tag="IVW84_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7917465.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6528..6980
                     /locus_tag="IVW84_001260"
     CDS             6528..6980
                     /locus_tag="IVW84_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7917466.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7069..9336)
                     /gene="gshAB"
                     /locus_tag="IVW84_001261"
     CDS             complement(7069..9336)
                     /gene="gshAB"
                     /locus_tag="IVW84_001261"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7917467.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9523..10521)
                     /gene="ftsY"
                     /locus_tag="IVW84_001262"
     CDS             complement(9523..10521)
                     /gene="ftsY"
                     /locus_tag="IVW84_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7917468.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10532..11341)
                     /locus_tag="IVW84_001263"
     CDS             complement(10532..11341)
                     /locus_tag="IVW84_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7917469.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11350..14931)
                     /gene="smc"
                     /locus_tag="IVW84_001264"
     CDS             complement(11350..14931)
                     /gene="smc"
                     /locus_tag="IVW84_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7917470.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14951..15637)
                     /locus_tag="IVW84_001265"
     CDS             complement(14951..15637)
                     /locus_tag="IVW84_001265"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7917471.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15985..17766)
                     /locus_tag="IVW84_001266"
     CDS             complement(15985..17766)
                     /locus_tag="IVW84_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7917472.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17792..18706)
                     /locus_tag="IVW84_001267"
     CDS             complement(17792..18706)
                     /locus_tag="IVW84_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917473.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18733..19695)
                     /locus_tag="IVW84_001268"
     CDS             complement(18733..19695)
                     /locus_tag="IVW84_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917474.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19695..20639)
                     /locus_tag="IVW84_001269"
     CDS             complement(19695..20639)
                     /locus_tag="IVW84_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917475.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESYKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20640..21644)
                     /locus_tag="IVW84_001270"
     CDS             complement(20640..21644)
                     /locus_tag="IVW84_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917476.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6077 a         4643 c         3848 g         7170 t
ORIGIN      
        1 ttatcgttca aaaaaagaat acacgcttgc atctagtact ctgcgagtta caaatcatcc
       61 gatcaaataa acattcgctg attattttcc gcaaaaaaag cagcatatcc acgataaatg
      121 gatctactgc tcttacttat ccttatgttt tacaaggtat tttcttttac acattactaa
      181 tgctgtctca atacggaacg attgtcatcg cttgttcaaa ttcctcttgc aaatgttgtg
      241 tttgataggc ctgaaaagct gggtgatggg cacgcaagag gacacgtaat ttcataggat
      301 catagctgtc tagcaactgt tctttcaaga caaaatctcg ggtcagctgc cattcataat
      361 aataaccaaa aggtctgcag ctgtaaatcg tgtgaccgat ggatttatct tcaaagcgac
      421 gatgcgtatg gccaaaaact acttgtttga tatttgaata acggtcaagt aattctccga
      481 atgattctga tcctaaaaat gcatttaatt tgttccaacg ctcataagga gcagattgat
      541 acatgataaa tgcccgttta ggaacgaaat gagtggataa aatcacctca agtttttttg
      601 cttccagctg gtctagcaaa ttttttgcag ctgcaagcat ttctgcattg acctcgagat
      661 ctgttttccc acgctggatg aaccgatcat accaatacaa acgcttcatt cgtacgattg
      721 cctcgtaatc tttcaattcg gaaaactgat agtcgtacca gccattcatc cctaaaagaa
      781 cggtctgctc atttaaagca atataccgtt catttaaaaa atgtgggtct ggaaaatggt
      841 ccatcatctg ttcattgggc acatctgcca actcgtgatt gccgaaatga aatgtcgtag
      901 ataatccttt attgacgaaa aattcgacaa cagctaggca acggtctact ttattagccg
      961 tatctccggc tagatgaagc cgagtgatct tgtgcttttg caaaacttgc caaagctgca
     1021 tcagctctga ttcgccgaat ttattgatgt ccacatgtag gtcgctgatg atcgccaatt
     1081 ttcccatttt atccctctag tcttttcgaa caacttgcaa gttgcctttt ctcaagccgc
     1141 ttggtccttt taattgtaaa ctattataag ttggttctaa agcaagtgtg gatggatcat
     1201 ccatgtaaag tattgcactg tctttgattt tgattggtcc aactggtgta tcagccacta
     1261 cagaataaag acttagatct gtttgtgtat cttttttgac aatgatcaat cgaaatgccg
     1321 tatccaattg gcaactgact tggctttctg caaatggacc gtcaccgttt tcgatatcta
     1381 ataaaagttg atcgtctgct tctaatttat tcttcaatac ttcttgtgct tcttttgtca
     1441 cttctaattt catctgtatc acctcatgtc atagaataat cactttattc atcagatgca
     1501 actaatttgt ctggttctga ctgttctcaa tttattggaa agtaagtcat tcgtttatta
     1561 tgatttctct tatcacaaaa gagacaactt tcccttttct tcgttcagtt tcttgaacgg
     1621 aaaaagaccc aaaaagcaac ctgttatttg tttaacctta ttcctttctt aaataacaaa
     1681 aaatactata aaaaataaaa aacctttaac aactattgga aaaagaatta ctttttgttc
     1741 acggattatt aacaactctt cggtagagta aacttatcaa ataaaagact tctttggagg
     1801 aatcactatg aaggaatata taaaaaacat ctattttatc gaagaaacac aaaatatcga
     1861 gggttcttat attgaagtaa aaacactctt tgtcaatgaa gataaaacca aagcattgga
     1921 tatttataaa aaattagctt caaaaaaaac gaatagtttt ggattgatat taagcgagta
     1981 taaaatcaaa gcagaagaga gttatttcta tcaattacta aaacggtgga gtaagcttcc
     2041 cgccgatttt tatcgaaaaa tgcagatcat caactatcaa ccgcttgctg agacacatgc
     2101 ctaaatagag gttgtgaaat cagtgctttg agctaatacc gaaggtcagc tgattgaaca
     2161 ttgtttagaa gagattgtga caactgcgat ttgctttgag taataagaac gctggacaga
     2221 aaatagcttc tcatattttc tagtctagtg ggcttattgc cgagaagcag cagatggaac
     2281 atcgtttaga agagattgtg acaactgcga tttgttttga gtaataagaa cgctggacag
     2341 aaaatagctt ctcatatttt ctagtctagt ggacttattg ccgagaagca gcagatggaa
     2401 catcgtttaa agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag
     2461 tttcgctttg tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat
     2521 cacactattt tttgccactc cccattctag aatcaacccg tatgatgtag ataatgaatt
     2581 cacgttgaaa aaagatttct caaataaata aaaaccatcc gtaaaatcat caatacgatt
     2641 ttacggatgg tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga
     2701 aacattattg ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct
     2761 tgattcacat cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag
     2821 acatctggtt caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt
     2881 tcattggcag aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact
     2941 acaaataaga tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga
     3001 gtggatgctt cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg
     3061 aatctagaac cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct
     3121 tttttgtcag tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct
     3181 aattgagttt tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc
     3241 tggaaagatt cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga
     3301 cttagatctt tttgaaaatc tctttgtcgg ccagaaatga tgattagatc tggctgaagc
     3361 tgattaattt tttctaagtc tggttctttt attccacctg ctgattctac ttttttatag
     3421 gatgaaagat actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca
     3481 agagcatcca tcgtatctaa agaaccattg tcgaatacga cgactttttc tggattattt
     3541 ggcacctcga tttggtctcc attactatct gttatcgtca atgtagtttc agtttgtgtg
     3601 ctgctttttt ttgtgtctgc ctgactggtg ttgttgctat ttccacatgc tgctaaacca
     3661 aatgatgcta gaatcaagat acctactact aattttttca tctcttctct cctaatgtta
     3721 gttttagaaa taaagacaga atctgcggcc ttcaatctca caaatccgta tgtccatttc
     3781 gtataattca tccaatacat ctttttttat catttcttgg gtcgttcctt gcgtaaataa
     3841 ctgtccatct ttcattgcaa caatttcgtc tgcataactt gcagcaaagt tgatatcatg
     3901 aagaacaata acaattgttt tcccgatttc ttgtactaaa ttttttaaaa tctgcatgat
     3961 ttgaaccgca aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt
     4021 gtcttgcgct agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc
     4081 aatcgattga tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc
     4141 ctcgtctttt gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc
     4201 tttcacggtg atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc
     4261 taattcattg gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc
     4321 ttttggtatc aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc
     4381 gataaaagca gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt
     4441 tccatggtac agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga
     4501 atcaattttg tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta
     4561 ttccagccga aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc
     4621 agaaagcttc cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata
     4681 tttgcgacaa taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc
     4741 aaaagactga cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt
     4801 actgcttgtt catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg
     4861 taccagcagc cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca
     4921 aagcttgcaa ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt
     4981 aaaaaggtac tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa
     5041 aaaagattcg ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc
     5101 atcagcccaa cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct
     5161 ccgataaaga agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact
     5221 gaaggagtca aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca
     5281 gctagcgaga caaggataaa tgcaaaaatt ttcttactcc tcaatgcaca tgcaaaatgc
     5341 cagtttccat atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc
     5401 ataccaataa aaatagatgg ctttttttta agcatatcgt cgtcccctta tcagaagata
     5461 aaggaagata gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa
     5521 aatcaacacg cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt
     5581 tacgccaatt gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat
     5641 cactccgata aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac
     5701 taagccaatg cctaaaaact gcatcttctg gtaatctatt cccagattta ctgcaaacga
     5761 ttcgcccaga ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg
     5821 aacaacagca tacaataact cataatttcc tcgtatcaca gtcgcaaaat tcccttgaag
     5881 ccatgaagtc atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc
     5941 aatgatattc cctaacatga ctccaatcaa cggaagcatc aattgatttt tctggaggaa
     6001 caatcgcgta agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa
     6061 ggatcggaca atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg
     6121 cgcactatcc atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg
     6181 catgatcaac ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg
     6241 caaacgagtc actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa
     6301 ttgtacattc gatactccga tgaacaatga aatactactt aaaataacta ataatgctac
     6361 tagtcgtccg tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt
     6421 taagtgaatc gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat
     6481 atgctataat cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg
     6541 atgtaaaaag ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac
     6601 agcataaata tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa
     6661 tgatccaacc aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt
     6721 ccatactctt gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa
     6781 cgaaaaacaa tagccgtttc actgtagtca acgattacgt cactgcaaat cctgaggaat
     6841 tcggattgct cttaacagca aaagaagcag taaatgaacc tgtgattgac atcgaaaaaa
     6901 aatacccaga agaaaactct tcgccagctg aaaaagaaaa accaaaaaag aaaaaaagct
     6961 tctgggatcg tttattttaa aacgaaaaaa cacataaatt tttacatgca aaaaaagctt
     7021 ttgatcagat tgacatcaaa taggacaatc tgtcaaaagc tttttctttt aaaatacttc
     7081 aggatataac aatttcaata cattcatcgt caatcttctg ccttttcctg caaaaggata
     7141 aacatgcata tgcatggctg gattgaaatt tgcttcgatg atcccatatg cttttttatc
     7201 agttgtagga tcgatttctt tatctggtat aatcaaatcg ataccactaa tcttagctcc
     7261 tagtgcttct accgcagaaa cggcgatttt tttgtaagct tcagaaaatt catcagtcat
     7321 atcgatcgaa tcaccacctg tactgatatt tgagttctcc cttagataaa caatttgatt
     7381 ggcttgggga actgattcga tcgtcaaccc ttgttctttc aacatcaatt gttctaattc
     7441 acctaattgg atcaattcta aaggtgcacg atgattcgtt ccacgcaaag ggtcactatt
     7501 tttttcttca acaagttctt tcactgttcg gatactgtca ccaataacat tagcagggac
     7561 tcgcaacatg atggcttgga cttctccgtc gatcacaaag aaacgatatt ccgttcccgg
     7621 cataaattct tctactaaaa ccgaactgtc ttcacgaaaa gcaatggcta aacctgcttg
     7681 ataatcttcc agactagctc cttccttgaa aatcgtgata cctaacccgt aattggttga
     7741 tttaggtttg atcacaaatg cctgctcggc aaatctcgga taagctttta ccgcttcttc
     7801 catggatgaa aattctgcgc ctcctggtac ccggaaccca gcttctttta agactttttt
     7861 cgttactgtt ttgttttcca tgataagtgg gacgatatag ctgtccttac ttgtcatgtt
     7921 tgcatttttc acatattcta catggtcttg gaattgcaga cgcaaaaatt gatccgactc
     7981 atctaatact ttgacttgga cacctttttg gatggcatcg aacataaata tctgagtaga
     8041 aagttccatt tcgcgaaaac cggccaactg atagggacgt tcatggcttt ctttataata
     8101 ttgatgtccc aatgccacag caacttcttg ctgactcgat agctgtgctt cttgatagag
     8161 tttcgctgct aacgtttgtt caggatgatc catttgagtc aatgcttcct ctactaaata
     8221 aagagaatcc aaccaatcca attcaaccag catttgcttc attccttcaa gaatttctct
     8281 accttcttga tgaaattctg tttgatctga tggttgttct agtgctactt tgttatttcg
     8341 agtcgtacct tctgctaccc attggtcaca gtcttctttt tcttctgtcc ataacatgta
     8401 catcaggaat aattgtaaga accgaacttg ctctggattg atacccaatc ttgcataagg
     8461 attcaaatcg atattccgca attcaatata acgaacacct gttgttgcta aatctgcgac
     8521 tttctttccg cctcggaaac gaagcggtgt ataaaattct ttctcctccg aaagtttccc
     8581 ttcttcgatc attcgctcga tatctgctaa atactgtttc atagaagcat aggaaacttt
     8641 cacatttgga tgattcgtat agcccagcgc actgttgcga atactgcgaa caggttcttg
     8701 cggatgtgat tcatctaaaa agtagttctt ctcactcatt ggtgaagcac cgaataaata
     8761 agtgatcatc cagcgatagc gcataaagtt tctcgctgtt ttcaaataaa gttctgtttt
     8821 gaattcggaa aaatcttgaa attcttcctg atggctgaac aatgttctta gcaactcatc
     8881 gccaaattca aaattgaaat gaataccgct taccatctgt tttcgcttcc cgtatttttt
     8941 tgctaaataa cgtcgataca agacatcttc gaaatttttt aattttgcaa taatgatctc
     9001 ttcgtctttt tccggtaagg caggtggcat acttaatggc cagatcatct cttctttcgg
     9061 tatagaacga gcagtcacat cataaacagc agcgagatac tgaaataatt cgggaataga
     9121 atcggtaaca ggggtgatca tctctgtttg tgtttcgcta aaatctgttt ggatataggg
     9181 gtgataggat cgatcgccaa agattgctgg atgatcggtt tttgctagat ttcctgcaag
     9241 atcaacccgc tgtccttccc gttctatacc aaatcgagct tggtcgataa aagggcgcgc
     9301 attgacatgc aataataatt gcttaaaatt catcattaag tgacctccaa ttcatatgaa
     9361 tctaattata atcaaaaagt ctaagaaaaa gaacgaaatt gtttgagaac cgcttacaaa
     9421 taagtataaa aatgaaggca tcaaaaacac agctatgtta aaaacaatca ttcaaacaag
     9481 gagctgcaac ttttgtcaca gctcctgctt tttagacatg ggttattctt cttttagtaa
     9541 tcctttgaac aatccaacga caaaatcatt tggatcaaac ggctccaagt catcgattcc
     9601 ttcaccaaga ccgaccaatt tcactggtaa atgcaattca ttacggatag caagtacgat
     9661 accgcccttt gctgtaccat ctaattttgt caaaacaaga ccggtcacat ctgttgtttc
     9721 tttgaattgt tttgcttgaa ccatcgcgtt ttgaccagtg gttgcatcta atacaagaag
     9781 tgtttcttgg ggtgcttctg gtaattcccg ctggattact cgttttattt tttctaattc
     9841 gttcatcaag ttcactttat tttgcagtcg gcctgcagta tcaactagta aaacatctgc
     9901 ctgctctgtc tttgctcttt ccaaagcatc gaatacgaca gctgctggat cgcctcctgc
     9961 attgccacga acaacttcaa caccagcgcg ttcgccccag acaactaact ggtcgattgc
    10021 tccagcgcgg aaggtatctg ctgctgccat caatactttt ttaccttcgt ttttgaactg
    10081 atgggcaagt ttaccaatac ttgttgtttt ccctacacca ttaacaccga caaacaaaat
    10141 aactgttagt ccgtcttctt ggatatttag ttcatttact tcattgattc cttccgcttc
    10201 atagagatcc acaagtttct cgatgatcgc attttggact tctgccgatt ttttcgcatt
    10261 tctgagtttc acttcttgac gtaatgcttc ggtgattcgt atcgctgttt caaaacctac
    10321 atctgcaccg ataagtgttt cttctaattc ttcaaaaaag tcttcatcga ctgttcgaaa
    10381 attggcaaac aactcattca tccgttcacc aaatgtttta cgcgtctttt ctaaaccctt
    10441 gtcgtacttc tcctgtactt caacttcttc tgttttctct tcgccagtaa aagcacgctt
    10501 gattttatca aaaaatccca tttgactccc cctactctaa tacaaatttt tggatcactt
    10561 tggctacgcc attttcttca ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag
    10621 gcacagcatt ctccatcgct acgcctagtc cagcatattg gatcatcggt aaatcatttt
    10681 cctcatcacc gatcgccatg atttcttctt gtttcaaacc aagatctttt gccaataaag
    10741 aaataccata agcttttgta acaccttttg gcataaattc caaaagcata cttcttgttt
    10801 tgatgatctc atacatttca tggaattctg ctggtatttc tttgatttta ggatctaatt
    10861 cttcttggtc ataagcgatt actgccttat taagcacaaa ttcatctgta atatcgctta
    10921 tatccgctgg ttggaattgc agcagcttat tcaacacatt gtacaaggat tgcttccttg
    10981 gggcactagg cagctgtaaa accacattgt cagacaaaac atctagtggc agatccaatt
    11041 gctgagccag tttgtataag cgttggatat ctgatacttc catcactttc ttttccatga
    11101 tttcaccagt atcatttttt tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt
    11161 catctaccag tcctaattct tgcagataat gcgccatcgc agctaagggg cgtcctgtac
    11221 atagaacgat cttgacccct tgctgttttg cttgtgctaa tgcttgttta ttctcatctg
    11281 aaatcagctt ttgttcattt aataatgtac cgtccaaatc gattgctact aatttgatca
    11341 ttttcgttcc tacactttcg tttcgttgat tttcgcctct tcattgatat cttccatccg
    11401 tacagaaacg atttttgata caccggattc ctgcatcgtc actccgtaca gtacatctgc
    11461 tgcgaccatc gttcctttgc ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc
    11521 gctcaagtaa cgaccaaaac gtttcacgtt ggcttcatct aacgctgctt ctacctcatc
    11581 taagatacaa aacggaacag gacagacacg gataatggaa aagagcaacg cgatggctgt
    11641 aagtgctcgt tctcctccag aaagcaaact tagactttgc agttttttgc caggaggttg
    11701 tacttcaatc tcaatacctg ttttcagcaa atcagttggt tccgtcagta ctaattcagc
    11761 tttaccgccg ccaaacatat ttggaaagac aattttgaat tcttgacgga tagcttcaaa
    11821 tacttctttg aatcttgtcc gcacttcatc atccatctca tccatcgttt caaacaattg
    11881 gtttttcgct gctaacagat catctcgctg tgttgctaag aatgtatgtc tttcactgac
    11941 ttgttcgtat tgttcgattg aatttaagtt tactggtccc agtttttcaa tctgttcttt
    12001 taaggatgaa acctttgttc tactgtcttc gatatcggtt gtttcttgat agtcttggga
    12061 agctttctca aatgtcagct gatattctga ctgcagatag gtcaaatgat tatctaaaat
    12121 catttcagct cggtcttttt ggacttctaa tttggattgt tccgacagtc gtgctttttg
    12181 ctcacgatta cgctctgcca agactgcttc aagcttatcg atctcctttt gccttctatc
    12241 tcgctgctct ttggcttttg ccaactcggc ttttaatgtt tctcgctgag cagataattc
    12301 gttaatttgt ttttccaaac tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa
    12361 tgtcgccaac tgcttttcta tcgcttcttg acgttcggtt gcctctgctt tttggactcg
    12421 ggcaccgcgg agctggatct gcagatgatt aaactgctct ttcaagacag caagatctgc
    12481 ttgttcttgt gctttttgac tttgtacttg tgctcttctg gcttccatct ggtcgctttc
    12541 ttgacttaac gatttgattt cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg
    12601 tttgtctttc aattcgttcg tttgcttttc gtaatcttcg ataaactgct gcagttcacg
    12661 tgtctcaaaa gttgagattt gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt
    12721 ttgtagctga tttgtcgctt cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa
    12781 aacttcttgt tcttcggcta aacgagctgt ttcttgttgc agctcctgaa cttttttttc
    12841 ttgtgcttgg agttgtttat ctgcttcttc aaattcacta gtcaactgtt tcaattcttg
    12901 attttgtaca aacaaactgc cttggtttcc tcgtttattc gctccacctg tcatggatcc
    12961 tccggcattc atcacatcac cttctaagga aaccactcga tattgatagc ggatcgtttg
    13021 cgcgattgca ttggcactag ttaagtcttt ggctaaaagg atcgttccta gcaggttttg
    13081 aaccacagtt tggatttgat cgggataact gacttgttca ctggcaatcc ctataaaccc
    13141 ttctacagct gctgcttgtg tcaatatatg agccggtaat tgtcttggtt tgattgtcgt
    13201 cagtggaagg aatgttgctc ttcctcctct ttgctgtttc aaatacgtga tggcttgccg
    13261 agcatctttt tcattttcaa cgatgacatg ctgggctgcc ccacctaaag ctgcttcgat
    13321 agctaacgtg aaatctgcag gaacatcgat cagttcagcc actgctccga caatacccga
    13381 aagctgctgt ttatgctgca agaccaatcg aaccccttgg taaaaaccaa aatagttttc
    13441 ttggatttct tgaaggcttt tcttacgagc acgtagctgc tggacttgat tcattaattg
    13501 atacatttta ggctgttctt ttgctaatgc ttcttggaac tttttcccgt tttgttggat
    13561 catttctaac ttctcttttg tttcagttaa agatgatttt agctgcgcct gcttttgtgt
    13621 caattcttct ttttgtaaga tcaatcggtc tactgatgct tctacttccg attgcttggc
    13681 taacgtctgc ttgctttttg ctgtttcttg tatatattga cgttctagat acttcagttc
    13741 gtttcctaca gctgcttgtt cttgcatcaa atcaacatat tgatctctta attctgccaa
    13801 taattctttc gttgattttt ggtatttttc taattcttgc tgtgttttta tgagatttgc
    13861 ttcagctttt tggatctctg tttctttttc tgctgctgct ttcatcaagc tttcttgaag
    13921 cttttcaaaa tgtttaacct ttttttgtgc ttctgctaat gaagtttgat attcttgcga
    13981 gcttttttgt gtatgctttg tccgctcctg caaaacatct ttttgtcctt ctgtctgctt
    14041 cagtttttcc gaaagatcta ataatacttg ctgatttttt tcgatcagac gatcggcttg
    14101 cgcattttct ttacgctgct tcgccaaaat ggattcttgt tcttggatcg actcagatag
    14161 tttgcctaat tctagattga atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt
    14221 tgctgtcttg atttcagcaa ccatcaaaga gacatcggtt tgagtcagtg tttcttttag
    14281 ccgtaaaaac tcctttgctg cctcactctg ggcggccaaa ggagttaact gttcttccaa
    14341 ttcgtggatg atgtcttgga cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc
    14401 tgctttcttt ttccgctgct tgtatttcaa gacaccagct gcttcttcaa agatcccacg
    14461 acggtcttcc ggtttactac tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa
    14521 cgattctttt cctaatccag aatctaagaa caattcttgg atatctttta atctgcagct
    14581 ttgtttattg atgaaaaact cgctttcccc agtccgtcga taacggcggg ttacactgat
    14641 ctcattgtac tccaatggca gataatgatc actattgtct aaaatcactg tgacttctgc
    14701 aatattcagc tgcttacggg tatcggaacc ggcaaagatg atatccggca tttttccgcc
    14761 tcgcagactt ttagcagatt gttctcctaa tacccagcgg atagcctctg taatattgct
    14821 ttttccgctg ccatttggtc caaccactgc tgtgacgcta ttttcaaaat caatgactgt
    14881 cttgtctgca aatgatttaa aaccggctat ttcaattcgt ttcaaataca aagctttccc
    14941 tcacttactt ttacagtgct gccaatgcat tttccgccgc agcttgttct gctaattttt
    15001 tcgatttccc ttgtccagtg ccaatcaatt gatcatctac ataaacttcg atccaaaata
    15061 ctcgttcatg agctggacct tcttcattga tcaatcggta ttcgattgat acatcacctg
    15121 atttttgcaa gacttcttgt agttttgttt tatgatccat ctcatgtgaa aaagcacctg
    15181 ctttgacttt aggaaaaatc actttttcga tgaatgcatg ggtcgtgtcg aatccctggt
    15241 caagatataa cgcgcctaaa aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac
    15301 gtcctcctga attctcttct cccttgccca acaaaacata ttgatcaaag tgacattctt
    15361 tggcaaattt cgacaagctg tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc
    15421 cttctggcaa atgaggatat aaacggaata aatactgaga aaccatcagt tctaaaacgg
    15481 catctcctaa aaattcaagt cgctcattat ccgataattg cagattgcga tgctcattca
    15541 cataggatga atgggtaaaa gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat
    15601 agttttcttt tagttctttt gtcagctggt tgtccatttt ccacattcct tttatggata
    15661 ttttttcgat acttccttat tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa
    15721 aggtaagtaa agctttaata atcctagtga ttgacgagaa gttgtgaaaa attcgtttag
    15781 tttcgagtat gaagaaaaaa ttttgtgaaa atggctttcc atatttttgc ataaatttga
    15841 cttaataccg agaaactgga ctttgaacac cgtttattgg agaagtcatg aaatcagcgc
    15901 cttgacctga atattagaga aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg
    15961 ccctttttcg gtcattttta gtgattatga acgtgaatct gctgtaacag aaacatttgc
    16021 ccatgggttt ggtacatcat aagaccaatc ccagtctttg acacggtcgt tgactggcaa
    16081 tacttcatta cggaacaacg ttggaatcgc aaatgcttct tcaaatgcat attgttgcca
    16141 tttttgatat gcttcttttt ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt
    16201 gatcaatttt gtattttctt ctgattcata acgagtgtag ttgaacgcag catttggtcc
    16261 ccatagtcca cttggatttg gatcagttcc tgtattccaa gcagcttggt aaacatcaat
    16321 ttcaggatca tcgttttcta atttatcata gaaagaattg aattcgatca gacgacctgt
    16381 tgtgtattgg acatccaaac caatttcttt ccattgttgg atatagtaat ctgccaatgg
    16441 ttgcgctgtt tccccaccgg acatcgaagc aaagttgatt gttagtttat ttccatcttt
    16501 gtcttctctt aaaccatcgt catccacatc tttgtagcca gcatcatcta atagtttttt
    16561 cgctttgtct agatcttgtg tgaatccttt catctcatca tcatggtatg actggaatac
    16621 tggaggaatc aatgaagtcg cattgcttct caaacctgca tagaaacgtt cacctactgc
    16681 attattatct aatgcatagc ccattgcttg acgtaatgat ttatcagcca tttttgaatc
    16741 tggattgtat tccacactct ttgtatctgc attccattta cctaacttga atcctagata
    16801 agtataagaa gtttgttcac ggcctaagtt tgtatagcca gcaatttctt tataggtatc
    16861 gtaagtatct gttggcattt ggaaaatcat atcatataat ttagagttca atgcttcagt
    16921 tgcacttgcc atcggtacag atttcatcgt gatctttttc aatttaggcg taccattcca
    16981 gtagtgctcg tttggcaagt attctactga ttctcctgca acgatattac tcatgtaata
    17041 agggccaaaa gtcactggat ttttacgtac ttgatcgctt gattccatat ctttgattgc
    17101 tacaccttct aacgtatgtt ttggtgctgc atatgaccag ataccaccac ccgattgaag
    17161 catgcttggg tttacttctt tatattcgat ttcaactgtt ttgtcgtcta tttttttgat
    17221 tccggagatt gtgtcggcac ttccatcatg atattcttcc atacctacga tgttacggaa
    17281 agtgtcgtca taacggatac cagtgtaatc tttattccca atgatttcgt aagggaaaat
    17341 cacatcgtcc gccgttacat cttcaccatc tgaccatttg acgttgtctt ttactttgat
    17401 tgtcgctttt ttcgtatctt catctagatc aagcgttgca gctccgtcgt ctgtgattct
    17461 gaaatcgtca tcgtttgcaa atagcggttc atgagaagga gccataaaag ctgcatcata
    17521 tgaatcttgg taaaactccc actggaacaa tcctttaaac tgactgtccg ttgcgaccgc
    17581 agcttctaat tcgccgtctt cgattgcatc atcattattt tttgttgcaa ttggaaaagt
    17641 taattcctct ttgttattgc cgttgcctcc agagttgttt ccagaggtat ctgtacctcc
    17701 gcccccgcat gctgcaagaa cgacagcaga catcagcgct gcaaatccaa aaatcgtttt
    17761 cttcattcct atttccccct tcgcttctat actagcctaa ccgttggcgc gcatctgcag
    17821 aacgcttgaa tgcttgtcca acataattga tactcaacat caaaactaaa ataaggattg
    17881 atgcgggtaa ccagatccat tgccgattca ctaagacttc tccgttactt gcataaccga
    17941 tcaatgtacc taaactaggt acatttgccg gcaagccgaa acctaagaaa gtcaaagttg
    18001 tctcgatacc gatgtttgcc gcaaagttca tcgtcaaatt agtaatgatt aatgaactta
    18061 gattcggcat gatttcgcgg aacatgattt taaaatcatt ggttcccatt gtttttgagg
    18121 cactgacata atctcttctt gcttcagata atgtcttgct tcggaataat cgggctttag
    18181 ctacccaata aaatgcactc atgatgaata taaaggacca gacattgtat ctggaaataa
    18241 tcgatacgaa gacgataatg atcagcatga tcggtagaat catgataaaa tcaacgatcc
    18301 gcatcaagat gttgtcaatg atccctccgt agtagccaga gatgatccct aatccaaccc
    18361 cgatgatcga agtaaggatc gtgatcgcaa atccgatcgt aacagagttt ctagcaccta
    18421 tgatcaattg tcctaataca tcacgtccgc cttcatctgc tcccagaatg aacttttgtc
    18481 cttgtgtcga aactgttcct ggtgcagcgt atttgtcgaa gatactaaca tacatcactt
    18541 tgtcttgatc ggtcagcatc gcaccgataa agatcactaa taataaaacc actagcaaac
    18601 ctaatgaaaa aatagcaatt ttatcttttt taaattctcg agcaatcatt cgtatcccca
    18661 taggaggaat actttcttga actgctgtat tgtttttttc actcatcgat ccattctcct
    18721 ttctgattgg ttttattgaa tccgaatacg cgggtcaacg atactcatga tgatatctga
    18781 aagtaatgtc cccactagcg tcgcgattcc taaaatcaat actaaggatg tgatcacggt
    18841 atagtcccgt tggccgatgg aatcaataaa tagttttccg actcccggat aaccaaaaat
    18901 cttttcgata actacagaac cagcaatcaa cgaagtgatt tcatacccca tttgagaagc
    18961 catcggcaac gctgcatttc ggaaaatatg tcgactgaat actttattcg tcggtacccc
    19021 tttggaacgg gctgttctga caaaatcgag cgatttcgaa tcgattactt cacttcgcag
    19081 atattggatt gtgacggcag tcgccaaaaa tgcttgggta attgatggta acagaatata
    19141 tttcaaacgg tctatccatt gagtgataaa tccaccttgc aaacctagtg tttgtgatcc
    19201 acttgttggg aaccagccta aacgataacc aaaaacaaat agcatgatca agccgaagat
    19261 aaataaagga acagcaaaac taaagaagtt gtagattacg acaatcttat ccaagattga
    19321 attttgatac cggccagcaa gcatccccag aggcacagca atcaaataag taaggacaac
    19381 tgtcaagatc gatagatata aagtgttgat gatccgttcg ccgatcaagt cagcaacaga
    19441 gcgtttgtat aagaaacttt gaccaaaatc gccttgcaaa gcatttccga tccaacggaa
    19501 gtactggaca taccatgggt cattcaagcc agctgcttgt cgcatctgtt cgatcactgc
    19561 cggatcctga tttggattga tcaaaccggt aaatggatcc cccggcatcg cttgggctaa
    19621 taagaagatc agaacactta aaataataac ttgcgggatc atcaataaaa cacgtcgcag
    19681 aatcgttttc cacattagct tgcacctcca tctttcaacg ccactttgtg tgtagttgtg
    19741 atcgtacgca aatcgtagac tcgaccagtc gcatcatagt attctttttg gttttggata
    19801 tactcttgtt ctactcttcg tcgattttct ttgtagcttt ccctattttc tacatcgatt
    19861 tgcggaatag ctgaaagcag acgtttcgta tagatatgtc gcgggtcatt ataaatgtct
    19921 tctcttgtac cgatttctac aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg
    19981 tgttttacta cacctaaatc atgtgaaatg aataaatagc tgaggccgta ttcttgctgg
    20041 attcgtttca tgaaattcaa tacttgcgct tgaacagata gatccagagc agatacaggt
    20101 tcatcggcta cgatcagttt tggattggta gcaacagcac gggctactcc tagacgttga
    20161 cgttgtccgc ctgaaaattc atgaggatat ttatatagcg catcttccgg catcccaacg
    20221 atatctagca gtccttttac ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt
    20281 cggatgggtt cagcaatgat atccaacact cgctttttag gattcaaact ggacattgaa
    20341 tcttggaaga tcatctgcac atccttgtta taacccattt ttttgcggtt gcttcgcttc
    20401 gttacatctt ttccttcata catgatttgt ccggctgtta ccttttctaa tcccacaacg
    20461 gcttttcccg tagtagattt tccagagcca gactctccca ctaaaccata tgtctttcct
    20521 ttttcgataa taaaatccac accatctaca gcatatacat gatccgttac tcgattgaag
    20581 aatccactgc gaatagggta atgaactttc aggtctttga ttgtgattaa ttctgtcatc
    20641 tacacctctc ctttttcgtc tctaaagtgg aaatgcttat agcaggagca acgtaccaag
    20701 tggttcggtg cgacttcatg aaggataggc tcttcttcat gatcgcttgc tggtatccat
    20761 ggaatacgtg gtgcaaaccg acaaccttta cgaggcatgt ttttcagaga aggaacgaca
    20821 ccttcgatca catggagttc tgcatcatga tttccttctt gtggtataga gttcagtaaa
    20881 gagcgtgtat aaggatgctt aggatgatca aacagttctt ttacacctgc tacttcaacg
    20941 aactgtccgc catacatcac tgccactcga tcagccattt ctgctacgac tcctaagtca
    21001 tgagtgatca agatgatccc tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga
    21061 atctgtgctt ggatggttac atctagggct gtcgtcggtt catctgcaat gacgattgga
    21121 ggtttgcagg cgattgcgat tgcgatgatg acacgttgtc tcataccgcc tgatagttca
    21181 tggggatatt ggcgtccaac tctcgcagga ttaggaatcc ctacttgttc tagcaattct
    21241 agtgcacgag cttgacgctg ttctgcattc atctttgtgt gataggaaag tccttctttg
    21301 atctggtctt cgattctcat gagaggattt aatgcagaca acggatcttg gaaaatcatt
    21361 ccgatatcat ttccgcggat tttattatag agcgtttcgt tcaggtcgat taaattcaga
    21421 tcgttatata aaatctcacc tgtaattttt gtgttattag gatcaagtaa tcccataatc
    21481 gtagtagcca aggtactttt cccgcaccca gattccccaa cgattgctaa aatctcgttt
    21541 ttgtctaaag taaatgaaac gtcatctact gcatcgtaat agtcatcttt gagtcggaat
    21601 cctgtatgta ggttctgaac ttctaataat tggttatcgg acaatcgaat acccctctct
    21661 gcttcctaaa tggaagtttt tcttaataaa aaaggacatt acacagaata aagcgtttta
    21721 taacaaaggt tattctta
//
LOCUS       DACCWV010000040        21732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCWV010000040 DACCWV010000000
VERSION     DACCWV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.040"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..1374
                     /locus_tag="IVW84_001271"
     CDS             34..1374
                     /locus_tag="IVW84_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7917477.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1592..2035
                     /locus_tag="IVW84_001272"
     CDS             1592..2035
                     /locus_tag="IVW84_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7917478.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2539..3957)
                     /gene="nhaC"
                     /locus_tag="IVW84_001273"
     CDS             complement(2539..3957)
                     /gene="nhaC"
                     /locus_tag="IVW84_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7917479.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4360..5583)
                     /locus_tag="IVW84_001274"
     CDS             complement(4360..5583)
                     /locus_tag="IVW84_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7917480.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5614..6453)
                     /locus_tag="IVW84_001275"
     CDS             complement(5614..6453)
                     /locus_tag="IVW84_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7917481.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6470..7150)
                     /locus_tag="IVW84_001276"
     CDS             complement(6470..7150)
                     /locus_tag="IVW84_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917482.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7152..8225)
                     /locus_tag="IVW84_001277"
     CDS             complement(7152..8225)
                     /locus_tag="IVW84_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917483.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8872..9543)
                     /locus_tag="IVW84_001278"
     CDS             complement(8872..9543)
                     /locus_tag="IVW84_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7917484.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9630..12308)
                     /gene="mgtA"
                     /locus_tag="IVW84_001279"
     CDS             complement(9630..12308)
                     /gene="mgtA"
                     /locus_tag="IVW84_001279"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7917485.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12967..13404
                     /gene="ndk"
                     /locus_tag="IVW84_001280"
     CDS             12967..13404
                     /gene="ndk"
                     /locus_tag="IVW84_001280"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7917486.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13426..14292)
                     /locus_tag="IVW84_001281"
     CDS             complement(13426..14292)
                     /locus_tag="IVW84_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7917487.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14452..16545)
                     /locus_tag="IVW84_001282"
     CDS             complement(14452..16545)
                     /locus_tag="IVW84_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7917488.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17294..17917)
                     /locus_tag="IVW84_001283"
     CDS             complement(17294..17917)
                     /locus_tag="IVW84_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917489.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18076..19473)
                     /locus_tag="IVW84_001284"
     CDS             complement(18076..19473)
                     /locus_tag="IVW84_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7917490.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19505..20476)
                     /locus_tag="IVW84_001285"
     CDS             complement(19505..20476)
                     /locus_tag="IVW84_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917491.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20850..21275
                     /locus_tag="IVW84_001286"
     CDS             20850..21275
                     /locus_tag="IVW84_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917492.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6264 a         4495 c         3772 g         7201 t
ORIGIN      
        1 tttgattgtg aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa
       61 cttagctcca gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt
      121 aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt
      181 ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc
      241 gacacgcatc acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat
      301 tttactgatt gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca
      361 gctgcttcct ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg
      421 ttggctgttt taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa
      481 tattggctgg cttttgctaa aatcagtgtc attatcttac tgatcatttt tgctacttat
      541 cttgtcggtc agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac
      601 catggtgggt ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc
      661 tattcttacg gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa
      721 cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt
      781 cctttgtttt tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt
      841 ccttttgtga tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt
      901 gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt
      961 ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag
     1021 catcaagtac cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct
     1081 ctttcttatt ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca
     1141 gtattattga tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga
     1201 acattatggg ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt
     1261 ggtatcctgt tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc
     1321 atttatttca gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga
     1381 tagaaaattt tattttattt atgtggaata gatggagcta ttgaagaaag gagttgtgac
     1441 aaaagtcttg ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg
     1501 tgttcaaaaa ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa
     1561 gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat
     1621 gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga
     1681 tcatatcttc ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc
     1741 agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat
     1801 cttagccgct agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac
     1861 tgtcaacgac acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctggc
     1921 aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca
     1981 ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt
     2041 atccgtccaa ataaaaaaag aggatgttcc agaagttatc agcttcaaga aataagaaga
     2101 agctacttct ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat
     2161 aagaacagcc aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca
     2221 gatgcaagct tttgaacacc gtttattaag aggttgtaaa atcagcgttc tgacctgagt
     2281 attagagaga caaatggaaa aatagttcct catattttgc cattttttga gctaataccg
     2341 agggtcagct gattgaacac cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg
     2401 tattaagaaa aattttgtga aatggctttc cacatttttg tataaatttg gcttaatacc
     2461 gagaaactgg cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca
     2521 caatcccttt ttatgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg
     2581 tttcacaaat ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg
     2641 agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac caaatgtact
     2701 tgctacgaaa cttccggcaa ttccccaagg cactaagtag ttgattactg ttccaccatc
     2761 ttctagcgtt ctacctaata cagttggatc aaatcctgct ttttcataag tttctttaaa
     2821 tgcattacca ggcagaataa cagataggaa ttgttcacca ataaaaatat ttactccgat
     2881 acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg
     2941 ttccatcacg gttgagatca agccaaattc gatcaacaat ccacctaatg acaaagttag
     3001 aataatcaat gacacagtag gcatcatact ttcgattcct ccgcggctaa gcagtgaatc
     3061 gatttgttgg tttccagtat ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt
     3121 taaggaagtt tgtgggtttt gaataaaaat cattcctact gctacagcga tattcaacaa
     3181 aatcgttaaa attgccggca cctttttcca tgcacacagt aacattaaaa ataaaggaat
     3241 cagtgaccag attgaaatag taaagtttgc ttctagaaca tcagtcactt cacgaataga
     3301 tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac
     3361 aaatgccggg acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac
     3421 aatagctgct gctagatttg ttgattcgga taatggagac atcttatctc caaaaacggc
     3481 acctgaaaca atagctccaa caaccagcgc tggatttaag cctagtgttg tcccaatacc
     3541 gaaaaatgca atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac
     3601 gattgcacac acaacaaata cagaagggac aaaccactgg acactgatca atttgaaccc
     3661 aatgaccatt agagttggaa tgatccctgc ttgtatccat acagcaatca atgaaccgac
     3721 taaaataaaa ataaagattg ggatgattcc tggtttgatg ccattgatga ttccttcgtg
     3781 cagccaatct gtcgggtatc tttttatcac tgcatacaac atgaccattc cgatcgctgc
     3841 taagatcgtg atattaggtg agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac
     3901 aataaccatt aatacaagtg ttgcttctaa aaaacttggt gttccttttt ttcccatgat
     3961 aatctctcct tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg
     4021 ccgcaattca tccgcgattc ctcctttata aaaataaaaa gacctgtttt aaactaagga
     4081 cgaatgactc cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacttt
     4141 tattcaaatg tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga
     4201 ccaccgactt tctgaacaaa acacctaacc gctactgcgt gaaaagttga cttgtacgaa
     4261 gtttaccttc cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa
     4321 aattattttt ataactttca cacacaagat tattttttac tagaaagtag cgccctcttg
     4381 tttttttctt gctactattt cttgatgttc ttgcttgatt ctttctaata gctttggctc
     4441 tttcaacaga tcaagagctg tattggcgag tagttctgct gcaatcgcaa tagaagcaag
     4501 gcctttgtca cttttagcag cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc
     4561 agagattgct accgtaggct ggattgttgg tatgacttgg ctgacatttc ctacatcaga
     4621 agaaccgaga cttgttcttt gttccgtttc tatttcttca tctggaacac ctgcttcttt
     4681 tgcatgctgg aagaataagt catcaaaaga aggagtaacg atgacatcat caactgcatt
     4741 ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt
     4801 ttccactttt tcatatactt tatctaatgt ttgccgattt cctgcacgta aatagaaacg
     4861 tccagcagcg taatctggaa tgacgttggc agctactcct ccgtctgtaa tgatcccatg
     4921 gatattgaca tcttttggca attgcaagcg tagagcgttg attccattaa acacttggat
     4981 caatgcttcc aaagcattta ctcctttttc aggtgctgct gctgcatggg aagcgacacc
     5041 gtaaaatttg atgtcgactg gatcattcgc taaagattct gtcgttttcc cataacgaaa
     5101 agctggatgg acgcataacg ctacatcgac atcctcaaaa aaaccttctc gaacaaagct
     5161 gcctttagct gatccgttct cgcccccttc ttcccctggg gtgccatata cacggatttc
     5221 tccaccagtt tcatcaatga attgcttcac tatgctggcg gctaatacgg agtaagtacc
     5281 aaacaaatta tgcccgcatg catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa
     5341 ggcaattgtg ggaccgggtt tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc
     5401 agctacttct ttttctaccc aaaacctttc tttttctaat tgtttaatca acgctgctga
     5461 ggaataaaac tcatagttac ttacttcagg atgttcgtgg atatctagag caagtgcttg
     5521 atattctggt aaacgatatt ggattagatc cttcaattgg tttgttgaga ttgttgttgt
     5581 cataaaaaat tcctcctatg ctagtcattt ttcttatttt ctcccgaatg tttcccatgc
     5641 aggcacgctt gatccttttg atgttttctc gattactttg actgtgtctt ctgtttgata
     5701 agcgtctact attttttgat acgttttatt gttttcatct tcttttcttg caacgatgat
     5761 attcacataa ggacgtgcat ggtcatccac tggttctaag aagatggcat cagaagcagg
     5821 attaaatcca gcatctacag ctacaccgct attgatcact gaaatgtcta catctgacaa
     5881 agcacgtgct gtttgggaag catctaattc agaaatgtct agattttttc tattctcggt
     5941 aatatcagaa acagtgggcg cttgtccttt tgccttatcc actttgatca atccagcagt
     6001 ttgcaataac agaagagctc tgccgccatt tgtaacatca ttagggattg ctacttttgc
     6061 tccgtctttg atatcagcta catccttcaa agtgttagaa taaattccca gcggtgcatt
     6121 gacagtattt ccgatcgata ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa
     6181 gaattgatgt tggaaagaat tcaaatcaat cgatccatct gctaaagcca catttggttg
     6241 attgtaatct gtaaatttca cgtattccaa attaatgttc tcctttttca aacggtcttt
     6301 cacgttgtcc catacatctg tatcttctcc aataatccct agttttacga tagtttcttt
     6361 tttgtcagtt gtatctgctt ttccttggct acaccctgcc aaccaaataa ttgctacaaa
     6421 tgtaatgact acaaaacgaa taattttttt cattttttct cctccacttt caatgtgtaa
     6481 tttttttaat cagttgatta ctgataaatt gacttaaaaa aactaagatt agaatcaata
     6541 atgtagccac aatcgttaca tctgtttgga atcgattata ccctctcgat atagcaagat
     6601 tccctaatcc gcctgcacct actgctccag ccatggtagt cagtccgatc acgttgatga
     6661 tagtcaacgc cgagacccga atgataccag gcaaaccttc taacaaatac acacgtaaaa
     6721 tgataccgat tggactggtt cccatcgctt ctgccgcttc gatcacccca ggatcaactt
     6781 ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac aactccaaaa atcaatggca
     6841 ctaaagctgc tttcgcacca atcgtcgttc caactaagaa acgagtagtc aatgctaaaa
     6901 gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcagctta tcaagtattt
     6961 caaatagcag acggttagct agtaatccat tagggcgtgt cactactaac gtcacaccta
     7021 agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa agacatatat agtgtttgct
     7081 tcgtcgcttc gacaaattca tccggaatca aataaacatt gggcaaatat gttttaaaga
     7141 aatcagtcat tttatcttcc tccttcgatc actttgatac gactatctaa atcgtttttt
     7201 tgtaaaatat ttatttttac accagatttg atcaaataat ccaatgcttt tttccgctgt
     7261 ttggtttctc cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg taataactct
     7321 acattgccat ataaaatatt cgctgttact tgatatttgc tgtacagatg agcgattaga
     7381 ggttcgttcg tctgatttcc aatataactt aattccacca gccatttatt ggcgatctga
     7441 tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt
     7501 cgaataaaat ctttggtcaa ttcttcttca ggattgctga agatctggat gctgctacct
     7561 ttttcaacga ttcttccatc ttccataacc gctaccttgt tgcagatttc tttgacaact
     7621 tgcatctcat gagtgatcag gacgattgtt aaacctaact ggcgattcaa tttttttaac
     7681 aatgcaagga tctgaagggt cgttttcgga tcaagtgcac tggttgcttc atcacataat
     7741 aataccttag gttcattcgc caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc
     7801 gacagttgtt taggataagc agatgctttt tcttccaaac cgacaagttc cagtagttca
     7861 ttcacttttt gacgacgttc ctgtttcgat tttccagaat actttaaaga aaaatcaacg
     7921 ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct gaaagatcat accgatggcc
     7981 tttctttctt ttcgcaattc tttgggagag agctgactca gattcgtctg attgacaatg
     8041 acttctcctg ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt acttttccct
     8101 gcacctgagt aaccgacgat tccataaaca tcgccttttt caatcgttaa atctacagaa
     8161 tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaacatc tcgcaattca
     8221 atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt
     8281 cttgaggacg cttttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc
     8341 ctcttcaagt acgatcatac tctagcaaga tatcggtcgc agccgtcatc gccttacatc
     8401 tatgctcggg atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca
     8461 gctaccgaag ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat
     8521 attcaaaaaa aaagtccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc
     8581 ggtaccacct tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt
     8641 ggcgttcgtt ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc
     8701 caaggccatt ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa
     8761 gtttccaaac ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac
     8821 atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat tttttctata tttatttttc
     8881 tgttttttta tttaacattt caataacagt tcgaagatct tttccttccg caagaagttt
     8941 caatttttcc ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac
     9001 aacgcctaat cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa
     9061 ggctgaacta gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg
     9121 cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa
     9181 gtgatccaag atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat
     9241 atgacgttct tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc
     9301 gcttgtcagc tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc
     9361 agggctacct gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca
     9421 agcgtcaacg atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc
     9481 atcacgatac gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt
     9541 catctataca ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa
     9601 gaacagaagc aaagaaccag ccttctcgtt tataagagtt cttggaatcg acggatatag
     9661 attttcttca cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac
     9721 cagtaattgc caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg
     9781 acagaaccga caataattcc taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt
     9841 tggataaatg gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt
     9901 gattcgacaa accatcctgc atggaataat gcgatgaaaa tcactcgctg tgaactatcc
     9961 aatgtatgga aacttccgcc cactacagca ggacaaataa caaaatacat caacaagtaa
    10021 gtagtaatat caaatatcga acttgttgga ccaaagtaaa ccatgaattc cccaatacta
    10081 gatgcattcc atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag
    10141 gaaatgcagg aaacatcata gatcagattc aaaaacaaaa gttgtaaagg cagcattggt
    10201 aaaaatggca ggaacgtact tgcaatcagc acggaaaaca tattgccaaa attagaactt
    10261 gctgtagcct tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct
    10321 ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata
    10381 tccacagctg tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg
    10441 ataccatctc ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt
    10501 gctttttgct gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat
    10561 tcatgatcac tcatttccga gattttctca ccggtaacca attcattgat ctctagacct
    10621 acttgacggc acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct
    10681 acaccatgtt ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg
    10741 aaagcaagat aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg
    10801 acgcttggat tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac
    10861 tgtacttttg caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca
    10921 agatcaataa atttagaaat gttcagcatt tcttcaatcg caccttttgt gatcatttgt
    10981 gttttaccag ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga
    11041 atttcatcca ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca
    11101 atgatagcca catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca
    11161 tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt
    11221 ttatcttgtg tcaatgtccc cgttttatcc gtacataaca catcgattgc accaaagttc
    11281 tggatggcat tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg
    11341 accaaattcg tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat
    11401 agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact
    11461 gctggagcca tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat
    11521 tcaaagcttg attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt
    11581 tcatttccaa cagaaacaat gattccttcc gcacttccac tgatgacatt acttcccata
    11641 aagacaagat tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc
    11701 ttttctactg gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg
    11761 gacaataaac gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca
    11821 cagacgattt cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt
    11881 actttcacaa gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac
    11941 gtcattgtcc cgctgacgat aaccatagtg aagataatgg ctacaccgaa aagatcttta
    12001 tctccaggag ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac
    12061 actaatgtaa atggcgtgat gtatgctttc aatatttcta caatgaatgg tgtttttttg
    12121 ccatatgaaa tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc
    12181 aatccatttg ctgatgcatg gaatagttca aataattctt gtttcgtttt tccagcaaaa
    12241 gctgcataga ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca
    12301 aaagacagct gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa
    12361 actgaaaggg gctgagaaca gatagataat agccctggcc tttcctctag atccttgttt
    12421 ttcgaatgac tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac
    12481 ttccaatcat ttttcgaata gttatttttc caattagacc acctcctttt tatttttcac
    12541 cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa
    12601 aagcatatta aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa
    12661 aaagcagata cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct
    12721 acccattggc gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc
    12781 taacaaatct tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat
    12841 caattttcca tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc
    12901 ggttttattt gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt
    12961 gctgttatgg aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc
    13021 ggaagcatca tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa
    13081 actatgactc ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt
    13141 gatgaactga tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag
    13201 gaagtgatcg atatcgtccg taagatggtt ggagcaacaa aagctgccga tgctgttcca
    13261 ggtacgatcc gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat
    13321 tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa
    13381 ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc
    13441 agcaatggcc agcagttttt tctctaagga cctccgttga ttctttccta acatatattc
    13501 gagcactgtg aagaattctt ggtagtcttt cgtcttttct tcccctaaag cagcagaaat
    13561 caatctttga taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg
    13621 tgctaagctc cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc
    13681 tatataaaac gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa
    13741 ctgtgctaat cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc
    13801 actgaatatc caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat
    13861 ctgcatcaag tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc
    13921 agatacttct gttttttcga ccatgtgaag attcaaaacc aaataataca gatcttctgt
    13981 tttcttaaaa tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg
    14041 tacccgatct agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg
    14101 catccattca tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac
    14161 aaatgacatt tttttcactg aatttgtctc aatcccgact agagaagtct taaaacagat
    14221 tccttccgct aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc
    14281 accatcatac ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat
    14341 acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc
    14401 aaccttcaag cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt
    14461 atttttttgc tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt
    14521 accatttttt gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt
    14581 acgttccgct tcatttttat agtcatattt tttcggatca atcgtctcaa atccgtttgg
    14641 cacataaaac cgcaagagat tttcctgatt cagcttatct gaaagactta acgcagtctt
    14701 gactttttcc tgatctttgg agatttcctc atcgattcct gctgctttcg tatcgatgat
    14761 ttctcctgtc tgattattgt aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa
    14821 gttcccattt cgaaaagcaa ctacttgatc atgctgagaa gataataagt ctgtaccaaa
    14881 atgaagataa tctttatcat cgattcccaa tagatgtagt aatgtcggca aaacatcgat
    14941 ttctccaccg tattcatgat tgactgtccc tttagtataa ccaggcatat ggatcatcaa
    15001 tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat ctttaccaag
    15061 agcgcttgct aaatctttat tatctgtatt cgagatccca aaatgatccc cgtaaatcac
    15121 aaagattgaa ttttggtaaa taccagattt tttcaaatac gtaaaaaatt gttccaatga
    15181 ttgatccaga taatgtgccg ttcgaacata attatctact gtgctattgc cagtattcaa
    15241 agatggaaag tcgaaattct tgtcatccgt ataataaggc gtgtggttgg ttacggaaag
    15301 aaatttcgtg taaaacggct gttgtagatg ctcaagatat tgggcagatt ctctgaataa
    15361 atctttatct aatatcccat aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc
    15421 aaagaaattg tcataaccta agtttttata tacatgatct ctattccaaa aactagctac
    15481 attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc
    15541 ttgaaagaca ttgtctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt
    15601 gccagtttcc aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaagaa
    15661 attgtcaaag ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc
    15721 ttgtccgtct accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat
    15781 cacatttttc cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt
    15841 tgtataatct aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt
    15901 cattcctgtt ttgattccgt cataaatagt atacgcatcg attcctagat atttgacaat
    15961 atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag
    16021 atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg atagccatcg gtttgccgac
    16081 agggttgctt ttgataggta ttttcttgac cttcaaccaa accagtagcc caatgaacac
    16141 agcgatatcc agccagtaga tgacatcatg gggcttcatc aaagaaaagg agcttcctga
    16201 caatccttgc gacactttcg aaaagcctaa tacagatttg actgtaatga aatctgtgaa
    16261 ttcccgataa aaaataatat tcaagtacaa caaaagcgta tttaaaatat caatgatcaa
    16321 aagaatagct aacgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa
    16381 ggtagtcgca attgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa
    16441 tgaaaaatct gcatagtagg caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa
    16501 aacgattgct aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct
    16561 ctattcacat tttttcttat tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta
    16621 tcttaagatt tttttattct ttctaaagat tattccgtcc acttttcaca gcataaaacc
    16681 gctgcgatgt aagcgattaa actaatgaag agataaaagt cttgtggtga ccgcttcact
    16741 ccacggtatt caaaagccag tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat
    16801 tcttgtcgca gctccttatt tcttgatgca ggacagcttt ttcacaactt cttccttaaa
    16861 cggtgttctc ctgccaactc ctcggccaca agtcacaata tttataaaac atgagaagct
    16921 gttttttgaa attgttcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
    16981 cacggtgttc aatcagctga ccctcggtat tagattatgt caggacgcta attttacaac
    17041 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    17101 atataaggag ctattttttg aaattgctcc ttgtggctca gagttaaacg gcaggctcaa
    17161 gacctcttga tacacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa
    17221 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaaac gctgatttca
    17281 caacctctgt ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa
    17341 taattgaggc ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag
    17401 gacttgttcc tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat
    17461 ttgtcttttt ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat
    17521 aagcggtgat cacttttcca tttttcaaac gggctacttt gtcgccatca tgagtcaaaa
    17581 gcaaaacggt caaatgcttg tctttaaatt gatattgtct gagagaaatc gcactaagta
    17641 cgtcatcgat attaataaat tgattttctt cttttccttt tggcaaataa atgtccaatt
    17701 cgcctttttc gtaattggca gttattttat actccgccca ccaataagtt gagaattctt
    17761 ttttgaaatt atctactgat tctttgcttg ctacgatttt ttctgtttct ttctgagcaa
    17821 gatctgctgt catttttcct gaaaatgctg cggataaaat aagcagtaag aaaaaaccga
    17881 ataaccaaaa gagaagaaac ggtctttttt tcctcatgct ttcctccact cctatttaat
    17941 tttgactatt ctattatata caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc
    18001 gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg gagaatttca cgcatttgct
    18061 ctcaactatt ctttcttaag cctcaaatcc gtttttatct gatacgtctt tgaaccaatg
    18121 acccgatttt ttgattgttc tttttccatc ttgtgctaag tctactgaaa taaagccata
    18181 tcgattctta tatgcattgg tccacgacca gttatccata catgtccaca tatgatagcc
    18241 taaacaattg gcaccttctt gaatggcttg atggacatat ttcagatgag attggacaaa
    18301 ttcgatccga taatcatcgt ggatcatgcc atcttctttg ataaatcgtg cttcgttttc
    18361 tacacccata ccgttttcag aaataaagca gtcgatattt ccataatgat ctcgtacatt
    18421 gatcaaaata tcatagattc cttgttcata gatctcccat ccgcgataag gattcatttt
    18481 acgaccaggc atttcataat tgtcgaaata atcctcaggc attggaccgt ttttagtatc
    18541 gattggtgtt tcttttgctt ttacccggcg tggctggtaa taattgatac ctaaaagatc
    18601 tactgtattt tctttgataa ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag
    18661 ttcttttact atctcaatca attcacttgg aaactcgcct tttacagctg gatcaaggaa
    18721 tgaacgattg aagaacgcat ctgctatttg cgctgcttta acatcttcag ggtttgattc
    18781 gtctcttgga tagcttggtg tcagattcaa gatgatacca ataacaccac cttgctccat
    18841 ttcatggtaa gatttgatgg ctaacgctga agccaacgct tcatgatagc caacttggat
    18901 cgcttttttc atatctgact cttctggata atgccatcga tataaataac ctgcttcgac
    18961 tggaacgatt ggttcattat gagtaaacca tttttttact ttcgaaccaa acaattcaaa
    19021 acaacttttg gcatattttt cataggcttc aaccgtttct cggttcaacc atccgccttt
    19081 ttcttgcaga tacatcggca tatcgaaatg atacagattc acaaagggtt cgataccgtt
    19141 ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg gcttcttcgt tgattgttcc
    19201 agattcatca ggtatcaaac ggctccattg gatagatgtt cgaaatgaat tatgcccagt
    19261 ctgtttcatc aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg
    19321 tcctacttga tcaaagaact tttgcggttc ttttttgtac caataatcaa aaatgttctc
    19381 tccttttcca tcgcctgaaa tccttccttc tgtttgtggg ccacttgctg cagaacccca
    19441 ccaaaaattt gctggaaatt gatatttttt catcaaagca cctctttttc ttttttagtt
    19501 cgttttagtt gattccatgt atgatatttt ccatcacaat cactttttca ttttcttcct
    19561 cttctttttt gattcgtttt aacagctgtt ctgctgctcg ttccccgatt ttatctgttg
    19621 gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt tttatctaaa taatcatagt
    19681 gactgaacgc aatatccttc ccgattctga caccttcttg attgaaacat tcgatggcac
    19741 caatcgacat attgtaattg ctgacaaaaa tagcagttgc ccctttttct aataatgctt
    19801 ttgccccgta atagccgctt tttctagaat agtctcctgt aaagacctct aattggatcg
    19861 atggatcatt ttggaccgct tctttggctc ctgctaaacg ttctcttgct acgtagtctg
    19921 tttgagtggc agcaataaat ccgatttttt tatgtccttg ttcaccatga tgagcgatca
    19981 cttttgctac actttctcga tcattcacta atattgtatc aatacctgct ggtccttctg
    20041 gacaattcaa tgcgataact ggaatactca tttttgtgag catttcaaat ccagaccact
    20101 cttcttcgga taagaagaca atcagcccat cgatcacatg cttcatcagg tattcaattt
    20161 tttgttcaat tttttctggc tgatcggcaa acccgcttaa taataagcta tacccatttt
    20221 cttcagctac tctttcgatc cctgagacaa cttccgaccc aaaacggctg gaaatcccgt
    20281 ttatcaaaag tccaatagag aagcttcgat tattcttcaa ccctttagca atgatgtttt
    20341 ctttatagtc caacgctgaa atcgcttctg ctattttttt catgttcttt tccttcagtt
    20401 gctgaccatt caagtaacgg gaaactgttc ccactgaaac accagccatc ttagccacat
    20461 cacggatcga tgccatacat tctcactcct gccagttaca tttgatttct ctttcttgtt
    20521 gttcgattat gatgaccgca ttctttgcat agtgttgctt gtcatcaata tccatataac
    20581 taaagtcttc agtagagcaa cttcctctca ctattaatta tacttaaaca agtatttgaa
    20641 ccggttcatt tttaatatag cattttttta aaagattgca acactttttt aaaacgcttt
    20701 ctgaaagtgg cggatttttt gcctttttaa aattttattc taaaatttac tctatttgtg
    20761 ggtgttttgt gcgtttttct cttccatccg tttgaagttt tacattccaa tggtataatt
    20821 tctttatcct atcaaatggg ggaagtattt tgaagaaatt aatcgtacta tttattttgt
    20881 ctgcgggact tttgacattg actggctgtg atttgtttgg cggctctgac caatcctcgg
    20941 acgaaacaac cgattcttct gtcttaaaag cggaaaaagc agataccacc aacagtgtat
    21001 atacgctaaa agtacgtaaa gatatggatt tatccgcggg acagttgaca tttacagata
    21061 aaaaaatgga atggacacgc acgtatcaga cacaaggaac tgattcttct gaatcatctg
    21121 aagaaaaaac agtgctgaca gatatcaaga tcactaccaa agatgacgaa tatattattt
    21181 caggaaaaga aaataaaaaa gaaacaacag tcatatttac aaaaatcggt aattaccgtt
    21241 tgaaggatga ggatggcaac atctacagtc tatagtgccg tctgttggat cttcttttgt
    21301 ttctcccctt cttttctgct atagtaaaga agcaagatga ttgtaaagga gacgaaatgg
    21361 atgatcgata gtcctgatta ttgtacatgt aataacaaca aaaatatcca tacagaattt
    21421 aatgaccaag gaagcttgag catatgcgac aactgtcaca aactagtcaa agattcattt
    21481 aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat
    21541 ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa actcagcttc ttcctttgat
    21601 caagcttcat cagtaaaaca aacagtggat catttcagtt tacaaatcaa agagtaaaga
    21661 tttatttatt tcaaatattt gaatataaaa taaccaaacg atagaaaggc ttattctaca
    21721 tcacgtagta at
//
LOCUS       DACCWV010000041        21666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCWV010000041 DACCWV010000000
VERSION     DACCWV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.041"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            52..1011
                     /locus_tag="IVW84_001287"
     CDS             52..1011
                     /locus_tag="IVW84_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7917493.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1178..2884
                     /locus_tag="IVW84_001288"
     CDS             1178..2884
                     /locus_tag="IVW84_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7917494.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(3042..3944)
                     /locus_tag="IVW84_001289"
     CDS             complement(3042..3944)
                     /locus_tag="IVW84_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7917495.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(3941..4645)
                     /locus_tag="IVW84_001290"
     CDS             complement(3941..4645)
                     /locus_tag="IVW84_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7917496.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(4676..5317)
                     /locus_tag="IVW84_001291"
     CDS             complement(4676..5317)
                     /locus_tag="IVW84_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7917497.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(5355..5669)
                     /locus_tag="IVW84_001292"
     CDS             complement(5355..5669)
                     /locus_tag="IVW84_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917498.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(5657..6286)
                     /locus_tag="IVW84_001293"
     CDS             complement(5657..6286)
                     /locus_tag="IVW84_001293"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7917499.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(6293..7009)
                     /gene="pyrF"
                     /locus_tag="IVW84_001294"
     CDS             complement(6293..7009)
                     /gene="pyrF"
                     /locus_tag="IVW84_001294"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7917500.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(7006..7938)
                     /locus_tag="IVW84_001295"
     CDS             complement(7006..7938)
                     /locus_tag="IVW84_001295"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7917501.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(7935..8711)
                     /locus_tag="IVW84_001296"
     CDS             complement(7935..8711)
                     /locus_tag="IVW84_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7917502.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(8765..11950)
                     /gene="carB"
                     /locus_tag="IVW84_001297"
     CDS             complement(8765..11950)
                     /gene="carB"
                     /locus_tag="IVW84_001297"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7917503.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(11955..13031)
                     /gene="carA"
                     /locus_tag="IVW84_001298"
     CDS             complement(11955..13031)
                     /gene="carA"
                     /locus_tag="IVW84_001298"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7917504.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(13036..14316)
                     /locus_tag="IVW84_001299"
     CDS             complement(13036..14316)
                     /locus_tag="IVW84_001299"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7917505.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(14335..15261)
                     /locus_tag="IVW84_001300"
     CDS             complement(14335..15261)
                     /locus_tag="IVW84_001300"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7917506.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(15274..16557)
                     /locus_tag="IVW84_001301"
     CDS             complement(15274..16557)
                     /locus_tag="IVW84_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7917507.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(16571..17110)
                     /gene="pyrR"
                     /locus_tag="IVW84_001302"
     CDS             complement(16571..17110)
                     /gene="pyrR"
                     /locus_tag="IVW84_001302"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7917508.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(17345..18256)
                     /locus_tag="IVW84_001303"
     CDS             complement(17345..18256)
                     /locus_tag="IVW84_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7917509.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(18253..18717)
                     /locus_tag="IVW84_001304"
     CDS             complement(18253..18717)
                     /locus_tag="IVW84_001304"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7917510.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(18722..19420)
                     /locus_tag="IVW84_001305"
     CDS             complement(18722..19420)
                     /locus_tag="IVW84_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7917511.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(19407..19793)
                     /locus_tag="IVW84_001306"
     CDS             complement(19407..19793)
                     /locus_tag="IVW84_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917512.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(19940..21610)
                     /locus_tag="IVW84_001307"
     CDS             complement(19940..21610)
                     /locus_tag="IVW84_001307"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7917513.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         6108 a         4863 c         3847 g         6848 t
ORIGIN      
        1 gtgctacact caaaatagtt ccgacgaaag aattataagg agtgagtaca aatgacctat
       61 aaccatctta caccaactga actcgtaatg atagaagctt atttcaatca atcacaacct
      121 gtttcaaaag tagccaatct gttacaacgt tctcgacaaa ctatttataa ggtctatcgt
      181 tttcttaaat ctggtggaag cgcaatgagc tactataaac aatataagaa aaacaaacgt
      241 aactgcggaa gacatccgat tgttttacct gatgagcaac aggaatacat tcaaaaaaag
      301 gttgttcaag ggtgggctcc tgatgtcctt attggtcgag cagagtttcc tattgcttgt
      361 tcggtacgca cactgtatcg aatgtttaaa gataaaacct ttgacactca cgatttacca
      421 atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc
      481 cggtcaatcc gagacagaaa gacagactat acccagtttg atcatgaatt tggtcacctg
      541 gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg
      601 ctatctaaag tcatcattac cttgaaaccc gaaggcagac aggctaaaga cattgaaaat
      661 aggctgaatc aatggtttcg ttgtgtccca aaaaacttat ttaaatccat cacatttgat
      721 tgtggaaaag aattctcaaa ttggaaaaag attagtaata ccaacgatat ttcaatttat
      781 tttgcggatc caggaacacc ttctcaacga ggattgaatg aacattcaaa tggcttatta
      841 cgtaaagatg gtttgtataa agctatggat tttaatggtg tatctgaaac ctttatccaa
      901 tcggtggcat cgaaacgaaa tcatattcct agaaaatcat tacactatcg gacaccaatg
      961 gaagtatttt tgagttacgt cacaaaagat gatttgtcta acttaatttg acaaacgaaa
     1021 atacctaaaa atttggattt caacgataga aacactgttt tttatgtaat tagtccaaga
     1081 tttttctaga cttttgcttt actattcggc caaattggtt tttcttcttt ttttcgttat
     1141 aatgaatata cttatgaaaa gaaatgagga atgaaccatg tcctttgatg gtgtatttac
     1201 ccacgcgatg gtcaacgaat tgcgtgaaac gttactatct ggccggatct cgaaaatcca
     1261 tcagccttat gaaaatgaaa tcgtgctggt gattcgttca cgcggcaaaa accatcgatt
     1321 attattatct gcgcacccaa gttatgctcg tgtacagatt acccaaatcg attatcagaa
     1381 tcctgataat ccgcctaact tcgtgatgat gcttcgaaaa tatttggatg gcgctatttt
     1441 agaagacatc gaacaaatcg aaaatgatcg agtgatccat ttccactttg ctaaacgaaa
     1501 cgagttgggt gatctacaaa atatcatatt gatcgttgaa ttaatgggac gtcatagtac
     1561 gatcgtctta gttaatcgag aaactggaaa aatactggat gcgatcaaac acattggaag
     1621 ctctcaaaac acttatcgtt ctttgcttcc tggtgtagaa tatgttgctc cgccaaaaca
     1681 agaggtcttg aacccattta gctccgagaa agaaaaaatc tttcagcggc tttcccaaac
     1741 tgaactagat ccaaaagcaa tccaacgtca gtttcaggga atcggtttcg atatcgctca
     1801 agagctgacc aaaagattac tggaacgtcc taatgaaaag atggttgtat gggacgaatt
     1861 tttctcagcc atcagccatc agcctattcc tactttttac gaaacggaaa ataaagattt
     1921 tttcacaccg attgcttatc aggtattgtc cgaacaggca tctgctgtaa cagcttatcc
     1981 aacactcagt caattattag atagctatta tcacgaaaaa gcagagaaag atcgggcaaa
     2041 acaacaaggc ggagaactga ttcgaaaaat cgaaaatgaa ctaaaacgaa ataaaaataa
     2101 attgaaaaaa cgtgaacaaa cactcaaaga atccgaaaac gctgagaatt accgtagaaa
     2161 tggcgaacta cttactactt ttctgacaca agtaccacgc ggcgcaaaag aagttgtttt
     2221 acctaattat tatgaagaag atcggccaat caaaatagca ctagatccgg cattgacacc
     2281 aaatcaaaat gcacagaaat atttccatcg ataccaaaag ctgaagaacg ctgttaagct
     2341 gattggcgaa caaatccaag aagccaaaga tgaaatccaa tatcttgaat ctgtcttatc
     2401 tcaactggaa attgccggcc caatggatat cgaagcaatt aaagaagaat tgacttcaga
     2461 aggatacctg aaaaagaaga acttaaaaaa acaaaaacgt aagaaaccat ctcagcctga
     2521 tcaatacttt tcttctgacg gaacgctcat tcttgttggt aaaaacaatc ttcaaaatga
     2581 ccagctgaca atgaagacag ctaaaaaaac cgactattgg cttcatgcaa aaaatatacc
     2641 cggttcgcac gtcatcatta aaagtgacaa gccctcagat gaaacaatta cagaagctgc
     2701 cgagttagct gcctattttt caaaataccg atactctgcc caagtgccgg ttgatcttgt
     2761 acaggtaaag catatccgaa aaccaaatgg cgccaagcct ggttatgtta tttatgaaaa
     2821 ccaaaagacg attatcgtga caccagaaga agaaaaaatt acagctatga aacaaaacag
     2881 gtaatcaatg agtgtttcta gaatcatatt tgcgtacttt cccacaacag caattagtta
     2941 taaattgttt gctacaaaaa gaggagttgt gacaaaattc tgtcacaacc cctctttttt
     3001 gatcaacggt gttcaataaa cagcgttctc atctgctttt cttagctctg ctggtccagt
     3061 aaccgagtaa gctctttgat aaatctttga gcgatttcac tgagcggacg tttcgccgaa
     3121 taaatgatat aaaattcatt tagttcgcct tctgccaaag ggattaaccg caagtgctcg
     3181 cgaatccctc caatcacaat accagatcct gaagcgtaag catcagtttc aagaacgata
     3241 ttcatcaaag tacctcgatc actcgtataa atcactgttt cttgatcagg gatctcgata
     3301 aggtcttcat aaaaatacgg gaaattattt ccatcctgtg taaaacgaac ttgaggataa
     3361 gataacaatt cttctttggt gatctctgcg tgttcagcta atggatgtcc ttgccttaaa
     3421 aaaatatgcg ttttgaattt tccaatgctg cggccactta gctctccttg ttgtaaatat
     3481 cttctgatac cagcttgatt atgctcattc atataaataa tgccgatctc actgtgctgc
     3541 tctttgactt cttcgatcac tctcgctgtt gtcgtctcca tgatccgaaa ttgtttgatc
     3601 tcgtctttga attcctcgag tagctgtgcg gtcaaaggac ctaagaaatc ataatgctgg
     3661 gcagcgatcg aaaaattgtc tctcttttca ctcgttagat agtgagtttc taatgtatca
     3721 agctggacca atattttctc ggcacgattt aagaagtctt gcccttcttc tgttaaatat
     3781 gcccctcgat tcgttcgagt aaacagcgtg atccctaact cggtttctaa ttctttgata
     3841 ccagctgaaa gacttggctg ggagacaaac attttctttg ccgcttctct gaaactacca
     3901 ttgtttgcta cagcgaccac gtatttcatc tgctgaatat tcattgctca aactcctcga
     3961 aataataaat ttctcttcca ttcaagggct ggattggtcg gttgttcggt cgagcatact
     4021 gaccatcgaa ggggagaaga aactcttggt tcagttttcc tacatgatcc attacaaacc
     4081 attggattgg cccgctggta ggcggagttg tcaatgagcc aatataatgg aaatggcttt
     4141 tttgctccgg aaagaacaca tccggattta cccaatgctc atgtttttct aagttccaac
     4201 tttccccatt ttcttgatta tacagccaac cttcctgtac cattagctgg tacatgacac
     4261 ccacaactag attctctttt ttgctgttca tatgaacaaa atgaaattca atatcttcct
     4321 gttttccgtt gatgatatgt tcagaaggca agtggaaatg aatatctgtc aaataatagc
     4381 gttctttctt gaactctaca taactgagtt ggtcaaaagg tactaaatga atcgtgtttt
     4441 tgaattcctt gtcagtaaac cgttgctttt gataatgaaa ggaaagcgtg tctccttcta
     4501 ttttggtttc ttcccccttg gtcaaatgaa taggcgactg caatggaaat tccgcacctc
     4561 tcgcaaacca atcacataaa ctcgcccaat tttccggacc tttgtctcca gaatagcccc
     4621 attcgacatc catattcttt ttcaactata cttccccctg catctcatat catttttatt
     4681 tttcttcttt ctttacaatc gttgagacgg tttctaaaaa cgcctctttt ccataaaatt
     4741 cctggttcaa tagttccgga aatatctctt taaagaaata ctgaacagaa ggccgatgtt
     4801 caaagctttt aatcgtagtc actgcctcaa tgaacatctc cttataaact ttttccgggt
     4861 tatttttgat gatttcatca aaagattcat aaagaaggtc aaccttatct gaaatggcta
     4921 atagttctcc ttctacggtt tcatcttttc cttcgaacaa ccgacggcga taaacttctt
     4981 ggaactcagc tggaatctct tggttgatga attcgtccgt catcttttct tcaactgttt
     5041 gcagcatccc tcttaactct ttgcttgcgt atttcaccgg cgttttgata tcaccaataa
     5101 aacgctctgt gtagtcatga ttcaatgatt tttcatagag cagtttccaa tcgatcggtt
     5161 gtccattcgc ttcttcaatg tctcctagaa cttgcgcaat agatgctaca cgatacgaat
     5221 gtgctgcaac tgtatgctct gtatatttaa aaaatccagg tgcccttgtg atcgtctcta
     5281 aattgttcaa tcctaaaata aattcattca accccataaa agttacctcc ctttgagatt
     5341 tctttttccg aatatcatat cattaaataa ctgatattcg caacatttcc gctctttacc
     5401 atttttgaaa cagctccctt accaaaaaat agagaaaatc tatatataat agaaaaaaga
     5461 gaagagatta ttctcttctt tctgctaacc agtcttagaa aaatgaattt taaaaaaatc
     5521 aaaaactgat cacgatagaa gtttcttttc atacctgatg aaaagatgct tctcgcaatc
     5581 agtttttgga tttttgctat tccaattgat gattctggag acacaaatcg gatttctcta
     5641 tcttcatcaa gcgcgcttaa atctgccaat tttcaggatc ttttttccat tcttttagca
     5701 gagttaattc ttgctcatcg atataacgat cttctaaagc tgcttcaatc aatacagaat
     5761 aattggtcaa cgtcataaga gggatttcgg ctttttcgaa atttgctttt ccttttggca
     5821 actcatacgt aaagatagcc gcaacaccaa ggatatccgc accttctctt tttgctgctt
     5881 cagctgcttc caagacactt ccgcctgtag aaatcaaatc ctcgatcaca accatcttct
     5941 gaccttcaaa tatacgtcct tcgatttggt tacctttccc atgatctttc gccttacttc
     6001 gaatatagac cattggcaaa tccagtattt ccgctaccca tgcggcatga gggattcctg
     6061 ctgttgcagt tccagcaatc acttgtactt cagggtaagc ttctttgatt ttgcttgcta
     6121 atccttttgc gatctctttt cggacttttg gatagctcat cgtgattcgg ttatcacagt
     6181 aaattgggct tttgattcca cttgcccatg taaaaggatt acttggattt aaaaatactg
     6241 cttcaatttc taataagtct tttgcgatac tttgttcaac attcatttta ggttagctcc
     6301 attctgtttg aatcgtttga tatgctttat aaggattagt tgactgagtg attggtcgac
     6361 ctataacgat aaaagttgag ccgatctttt ttgcctgaga aggtgtgact actcttgtct
     6421 gatctcctgc gactgttcct tctggtctta tacccggagt taagcaaaca aattcgtctc
     6481 ctgccatttc tttgatcgct tctacttccc aagcagaaga aaccacacca tctaatcctg
     6541 cttttttggc acagctagca taatggatca cacttttttc taaagaaact tcgattagtt
     6601 gatcggcatg catttcttct tcacttgtag aagtaagctg agtgatagct agaagtagcg
     6661 gacgttttcc accttctgga gtcccctctt ctaatcctcg cattgccgcc tccatcatac
     6721 gaataccgcc agcggcatga acacaggtga gatcaacacc aagctttgcc aatccccgca
     6781 ttgctttttc gaccgtattc ggaatatcgt gaagtttcag atctaaaaat acattatgac
     6841 cagcctcttt tagataacgt acgatttcag gtccttcttg gtagaacaat tccattccga
     6901 ttttcacaaa caatttttct tctttcggga aaaatcgaag aaagtcttct acctcttgcc
     6961 tagttgaaaa atctaatgca ataattggtc gttggtctag ttgattcatc gttcacgttc
     7021 ctctctcact tctttgatta atctttccag cgattcgatg cctagttcgc tcattcgcac
     7081 agggagctct ttaattagtt taggacagat atacggatcg gtgaaattcg cggtccctat
     7141 agctacagca cttgctccag ccataaacat ctcaagaaca tcatccactg tctgcacgcc
     7201 gcccatccca atgattggaa ggctggatat acttgctact tggtgaatca aacgaatagc
     7261 gacaggcttg attgctggtc cggagagtcc acctgtttga ttggctaaaa tcggttttct
     7321 tgtcttaagg tcaatccgca taccaagaag agtattgatc atcgtaaacc catctgcccc
     7381 tccagcttcg attgcctgag cgattggaac gatgtctgtt acatttgggg acaatttgac
     7441 atagacagga acagaagaaa ctttctttac tgcttgtgtt aattgaaaag caacatcaga
     7501 atctgttcca aaagcaattc cgccatgttt gacatttggg caagaaatgt tcagctcgat
     7561 tgctttcaca tttggtgctt cgccaatttt gctacaaact tctacatagt cttcttcaca
     7621 agcaccagca acatttgcga tgatgggtaa ctcattgtac tcctctaatt ctggaagaag
     7681 tttatgcata acaacgtcta gaccaggatt ttgcaaacca attgcattca acatgccaga
     7741 tggtgtttcc gccactcttg gcgtgggatt gccaaagcgg gcatttggag tcgttgcctt
     7801 gaccatgatc gatccaagct gattcaaatc atagtatttt ccgtattctt tgccaaaacc
     7861 gaaacagccg cttgccggca tgataggatt ttttaattct aggccaggaa gtttgaccgc
     7921 taaaggattc tcattcatat aattacctct cctattttaa aaatcggtcc ttcgtcacaa
     7981 actttcacac tttttgttcc tgtttcatct tccggcgtat gacaaacgca agcatagcaa
     8041 gcccctatcc cacatgccat gcgggattct aatgataact ggacattttg atgcgaagaa
     8101 aacagctgtt ccactgtctt caataacccg tttgaaccac atgcaaaaac agctgtaggt
     8161 tccgtcttcg ttcctaacaa taaattaccg acatttccgt gcataccgta tgttccatcg
     8221 tcagtagcga ttcttgtttc tcctagacga ttaaattctt tttcataata aacgacttca
     8281 tgtgtggcaa aacctaaaaa atgcgttggc ttaattcctt tttcgactaa ctgttttgat
     8341 aactcatata gaggagggac accgattcct ccaccgatta taaatacttc gtctcctgta
     8401 tgaagattgg acaaatcaaa accattgcct aaaggaccca tgacatctaa atggtctccg
     8461 gcagaaagag tagagaagac tctagtcccc tcaccttcca cccgataaat gatacggcaa
     8521 gtatttgttt cgtggtcgat atgattcaag ctaatcggtc ggcgtaatag caagtctgca
     8581 cgcggcacga gaatatgcaa gaactgtcct ggttgctgca tttcttttac tagatcacct
     8641 ttcaatgtca tttcaaaaat ctttggtgcc agctgttttt gctgaacgat ttccatcatt
     8701 tcttgtttca tctgtcccct cctgcaacgg tttttcgtct ttttgtctta acatgtgctc
     8761 gccgttaaat agggttagtt gaaaaagcac gggattccat cactcgaacg atcgcatctg
     8821 ctgtgtctaa agaagtaaac aatggaatac catgttcaac tgcttctctt cggatgatga
     8881 aaccgtcttt agaagcattt ccccgatctt tatcaatcgt attgacaacc acttgtgttt
     8941 taccatctct tattaagtcg actacatttt tttctgctgc ttcctcagaa attttcgcta
     9001 ctgtttcaac gaccagacca tttttttcga aataagcagc tgtatgtttc gttcctatca
     9061 gactatagcc aatttctgta aaacgtttag ctaattctag tgcttcttct tttgtttcgt
     9121 ccgcaatcgt aaataatacg gctccatatt caggcaagtg taatccagaa gcttcgaatg
     9181 ccttgtacaa agctttgtct aattgtctgt cagatcccat tacttctcca gtcgatttca
     9241 tttcaggccc taagtaagta tcaactttct gcaatttcgt aaaagaaaag acaggtgctt
     9301 taacatgaac ttcttttgtt tctggataaa gtccgttttc ataaccaagt gtctttagtt
     9361 tctctcccaa aatagctttc gtcgcaattt gtgccattgg aattcctgtc accttactta
     9421 agaatggaac agtccgactt gctcgaggat tgacttcgat cacataaacc tcattttcat
     9481 ggatgacaaa ctggatattc atcatcccga cacaattcaa acctaatgct aagcggatcg
     9541 tgtaatcttc gatcgttttc ttgatcgatt cagataatgt ttgaggtgga tacacagcca
     9601 ttgaatcccc tgaatggaca ccagcacgct caatatgctc catgatccct gggatgagta
     9661 ctgtttcacc gtcacaaatc gcatccactt cgcattcacc accaagcaag taacggtcca
     9721 ctagtactgg atgttctgga gatgctttca cagcatgttc catgtaatct tctaaatctc
     9781 gctggttttc aacgatttcc atcgcacgtc cgcctaatac atagcttgga cgaacaagaa
     9841 ctggataacc aattctcgaa gcgataacca cggcttcttc tttacttgta gcagtatccc
     9901 ctaaaggttg aggaatatcc aactctttca gcgcttgctc gaataaatca cgattttccg
     9961 cacgatccaa gtcttcgatc gttgtcccta agatttttac accagcagca gtcaaaggtt
    10021 cggctaagtt gattgctgtc tgaccaccaa attgaacgat tactcctatc ggctgttcta
    10081 gctcgatcac attcatcaca tcttcgaagg tcaatggctc aaaatataat ttatccgaaa
    10141 tagaaaagtc agtcgaaact gtttcaggat tgctgttcat gatgattgct tcgtatcctg
    10201 cctgctggat tgctttgaca gaatgcacag tcgcatagtc aaattcaacc ccttgtccga
    10261 tacggatagg accagatccc agtactagaa ttgagggctt ttctgatcgt gaactttcat
    10321 tttcatattc ataggaacta taaaaataag gtgtttttga ttcgaattcc gctgcacaag
    10381 tgtcaaccat cttatataca ggaatcaaat cattttcttt tctcatctgg cgaacggctt
    10441 cttcgtttat ttcccacagc tccgccactt ttttgtctgt aaaaccgttt tgtttcgctg
    10501 ttttcaatat cttgtcatcc aacggctgct gtttcaactg ctgttcgatt tctacgatat
    10561 gcaacagttt atctaaaaag aataaatcga tttttgtcaa atccgctaat tcttctatgg
    10621 tgtaccctct tctaatagct tcagctacat aaaacaggcg atcatcttgt gctcgaacca
    10681 ctttttggat taggtcatcg tccgtgatgt ctgccaactc aggaagttcg atatggtgtg
    10741 taccgatttc taaagaacgc acagctttca ataaagactc ttcgatattc cgaccgatag
    10801 ccatcacttc accagttgct ttcatctgcg tgcctaaaag ccgttctccg ttttcaaact
    10861 tatcaaacgg ccagcgagga attttagcta caacataatc taatgcaggt tcaaattctg
    10921 caaaagtcgt tcctgtaaca ggatttttca tttcgtctaa tgttaatcca agcgctattt
    10981 tagcagctag cttagcgatc ggatagccag tcgccttact tgctaacgca gatgaacgag
    11041 aaacacgcgg attgacttca atcacataat aattaaaact atgtggatca agagcgagtt
    11101 ggacattaca gcctccctcg atcttaagtg ctcgaataat cgaaagagaa gcatcacgaa
    11161 gcatttgata ttcatggtca gacaatgttt gactaggggc aaatacgata gaatcacctg
    11221 tatggatacc caccgggtca aaattttcca tattgcagac aacgattgca ttatcagccg
    11281 aatctctcat cacttcatat tcaatttctt taaaacctgc aatacttcgt tcaatcaaac
    11341 attgggtagc gggggaaaga cttaatccat tttctgatac gatacgtaat tcttcttcat
    11401 tttcacacat acctccacca gttccgccta aagtaaaggc tggacgaacg atgacaggat
    11461 agccgatttt atttgcaaaa tctacagctt cttctactgt attgacgatt tcagactcgg
    11521 ggattggctg attcaattct ttcatcaact gtttgaatag atcacgatct tctgcttgat
    11581 cgatcgcaga aagctttgtt cctagaagtt ctatgttcaa ttcttctaaa atacctgatg
    11641 ctgccagctc cattgccata ttgaggcctg tctgtccgcc taaagttgga aggagagcat
    11701 ctggacgctc ttttcgaaga atccttgata caaattcaag tgtgatcggt tcgatatata
    11761 cttgatctgc cacttcttta tccgtcatga tcgtcgcagg attagaattt actaaaacca
    11821 cttcatatcc ttcttctttt aaggcaaggc aggcttgtgt tcctgcgtag tcaaattctg
    11881 cggcttgacc aatgatgatc ggaccagatc caatcaccat aattttcttg atatctgtac
    11941 gtttcggcat ctgtttactg ctcctttcct gcgtccatca tttccataaa ttcatcaaat
    12001 aaatgtagtg catcatgcgg accaggggca gcatctggat gaaactgcac actgaaagcg
    12061 ggataatctc tgtgtttgac tccttctact gttccgtcat ttacttctac atgagttacg
    12121 atgagttttt ctggatcaat cgtggcttca tcgactgcat agccatgatt ttgagaagta
    12181 aaatcaattc ttccggtcgc tacttctcgt accgggtgat ttaatcctcg atgtccgaat
    12241 ttcattttat aggtatctgc accgttggct aaagcaaata actggtgacc gagacaaata
    12301 ccaaaaatcg gtacttttcc ttggattttt ttgatcattt cgattgcttc tggcacatct
    12361 tttggatcac caggtccatt cgtcagcatg actccgtctg gtgatagcgc taaaatcgtc
    12421 tcggcatctg tgttataggg aagtactgtg aggttacagt ttcttttact caattctcgt
    12481 aaaatactat gtttcaagcc gaagtcaatg acaatgactt tccgacctgt tcctggactt
    12541 ggataaggtt tgttagttga gacttgctgc acttgattgg ttgctagaac ggttgctttc
    12601 agctgatcaa atgcatgttc gaaggaatct ccagcatcaa ctatactagc tttcatcgtt
    12661 ccgacttcac ggattttgcg agtaagggcg cgcgtatcga tacctgatat tccaggaatt
    12721 cctttttgtt tcaaaaatga atctagcgtc atttggtttc gccagtttga agctacacgt
    12781 gcatgttctt ttactactac tcctttgcat gttggagaaa tcgattcata atcatctcta
    12841 tttaccccat aattacccac cataggatag gtaaatgtaa tgatttgacc gttaaaactt
    12901 tgatcagtga tagtttcttg ataaccagtc atacttgttg taaaaacgat ttctccgaag
    12961 acactagcat ctgcaccaaa agcctttcct tcgaaaactg ttccatcttc taatataagc
    13021 aaacgttcca tacttttatt ctcccttctg ccacacaagt tttccgttga caaaagtcat
    13081 ttgtgtttcg ccttttactt tccagccgat aaaaggtgta ttctcccctt ttgataaaaa
    13141 gtcttcttta tcgatcgtgc aagtttgtgt aatatcaaaa actgctacat cagcgggggc
    13201 accaaccgtt agcgttcctg cattcaaacc aaaaatctca gccggtttaa ctgccaacca
    13261 attgatcact tgttccaacg tgaatcgacc agtttctaca aaatgagtat agatcagttg
    13321 aaacgctgtt tcgcttccta cgataccaaa tggtgatttc atgaaacttt ggcttttttc
    13381 ttctaatcca tggggggcat ggtctgtagc gatgcaatcg atcgtaccgt caagcaagcc
    13441 ttcgatcaaa gcttctctgt cctcccgtcc tcttagcggc gggttcattt tccaaaagcc
    13501 gaaatcttcg ggaatatctt cgtcgatcaa aataagatga tggggtgaaa cttctgctgt
    13561 tacgtggatt cctgcttttt ttgcgtctct gattacacgg acactttctt tagtagaaac
    13621 atggcaaaca tggtaatgaa caccagttgc ttcagctagc aacagatctc tagcaatttg
    13681 agaagactcg gttacgctta agatcccggg aagaccaagt tcttctgcct tcttgccagc
    13741 gtgcataacg ccgccaaata ataaagattc atcttctgta tgggctacta gggctttatt
    13801 gttttttgca gcttctttca tagccagata catcgtccca gctgtttgga cacctacacc
    13861 gtcatttgtg aaagcaaaag caccttcttc aatcagtgct tcttgatcaa ctaatttctc
    13921 acttcgcaga ttttctgtga ttggcgcata ctgcaatacc ttcacaactg catcttttct
    13981 aatgatgtca tatacttgtc tcagcttttc tgccgtatca ggtacaggat ttaaattcgg
    14041 catcgcgcat acagtagtga aaccgccacg agcagctgag cgagtcccag cttcgatcgt
    14101 ctccttgtat gtaaatcctg gctctctcaa atgaacatgg acgtccacta aaccaggtgt
    14161 gatcaattgt ccctttgcat caaacacttc atcaaattcg gcttctgaga atcctgtacc
    14221 gattgctttg attcgcccat cttctatcca aatttctgct ggtgtcgtca tattttttcg
    14281 tgtattgatc tgaccgtttt taatgagtgt tttcattttt tcatcctcct ataattacgc
    14341 ttttccattt agcactgctt ctaagattgc cattctgacg aatactccat tgctcatttg
    14401 ctgaacgatc cgcgactgta atccttccac taatgaatca gctaattcta catcacggtt
    14461 aactggtgct gggtgcataa tgattgcatg tttttgcaac ttcttcgcac gctcaactgt
    14521 taacccatgt tcctgatggt agccttcttt tgagaaactt tcttttccat catggcgttc
    14581 gtgttgcacc cgaagcatca tcatgacatc aacttgatct agtagttcat ccaatggcat
    14641 ataatgtccg tacacttcga attcatcgtc gtaccattct ctaggacctg agaagaatac
    14701 ttgtgcgcct agacgtttta acatctgcat attggattta gctacgcgag agtgagtgat
    14761 atcacctacg atggcaactt tcagaccttc aaaatgacca aattcttcat aaatggtcat
    14821 caaatcaagc aagcattggg ttggatgctg tccgcttccg tctcctccat tgataatcga
    14881 acactggatt gttttacttt gtatcagttc gtcataatag ttctcatcac catgacggat
    14941 aaccgctacg tctacgccta atgctgacat cgtcagtact gtatcgtata gtgtttcccc
    15001 tttttggaca gagcttgtgc ttgcttcaaa ttcaatcact tctagcccta gttttttctc
    15061 cgctacatcg aagcttttat gtgtgcgagt actattttcg aaaaagaggt ttgtagcaaa
    15121 gtactggcgc ttttgcggag tccaattagc accttgcttg aattcctgcc ctctgcggat
    15181 caagcccata acttcttgat cagtaagtgc ctcaacggtc aataaatgct ttaagctgat
    15241 acgttctgat ttgatgatca taatgtttcc tccttagttt tcttcgcttc gtgaagtttc
    15301 tggtaagata agatttaaaa tgatgccaag tacagttgct aatgccattg ccgacaatgt
    15361 aaatgtgcct acttcaaaga cgagtccgcc aattcctact actaagataa ctgaagcaat
    15421 caacagattt ttctttttat caaaatcgat tttgttctcg attaaaatct tcattccact
    15481 tgcggcaatt actccaaata agataaaact gattcctgag atgactggtc cagggatact
    15541 taaaatcaaa gcgcttaatt ttcctacgaa tcctaaaatg actgcaaata ctgcagctcc
    15601 accgatcaca aacacgctat ggacacgtgt gatagccaga acaccaatgt tttcaccgta
    15661 acttgttaca ggaggacctc cgacaaaggc tgccacgatt tgtgctaatc catcacctgt
    15721 caacgtctta tgaagacccg gttcttcaaa gaaattacgt ttagttagtt tattcaatac
    15781 cataagatgg ccgatatgct ctgtcatcgt cacaaaagcg atgggtgcca ttgttgtgat
    15841 tgctccaaga tatagctttg gttcatattg tacaaaggga atctcaaagt taggaacagc
    15901 aaaccaggca gcgtctacga ttggcttagt atccacgatt ccaaacagca aggctacgat
    15961 gtatccgcta ataataccta gtaaaatcgg aatcaatccg agaaaacctc ttagccacat
    16021 gttgaagaaa attgttaatc ctaatgtgat cagcgctaca gcaatatact tgaaatcata
    16081 cgtgccctga ttatacatag catttgttgc tgcatttgcc gcaagtccca acccgataac
    16141 catgatgact ggaccgacga cgattggagg caagatttta tccagccatg ctgaaccgat
    16201 ctttttgatg atcagcgcaa caatcaaata gactaatcca gttgtgactg ctccttgtgc
    16261 aatcgctgga taaccatctg ttttcatcaa aagctgcatc gctgcaatga aagcgaagct
    16321 gcttcctaag tacgcaggta tctttccttt agtagtaaat aagtacacca atgtacctag
    16381 tcctgaactg actagcgcaa tacctggatc gatacctact aagatgggga ctaacacggt
    16441 tgctccgaac atcgtgaaca agtgttgtaa acttaatcct acccaatgtc ctggggttgg
    16501 gcgatcgtgg atatctaaca ccacgtcatc attatgaaat ccttttttct ccgacatgtt
    16561 tctctctcct ctaatcttcc tctttcaaaa tcatgatgcg atcttgtcca tcaagctctt
    16621 gcatctctac taaaatctct tccgcccgag atgttggaat gttcttccca acataatctg
    16681 cacggattgg caattctcga tgcccacgat cgaccaggac tgctaatgaa atttttcttg
    16741 gtctgccaaa atccattact gcgtccattg ctgctcgaat cgttcgtcct gtatacaaaa
    16801 catcatcaat caaaataact tcttttcctt ctaaggaaac tgggatgtca gaagaatgaa
    16861 gttccggttc ttctggattt tcttttttgt catctcggta taaagtgata tccagttctc
    16921 ctacaggaat atcgatatct tctagctgct tcaggcgttc ggcaattctt gatgcaatgt
    16981 agattcctcg tgttttgata cctaccaata cgatatcttg gatactatgg tttctctcga
    17041 taatctcata agtaattcga gtcaatgctc gcttcatggt tacctgatct actacttctt
    17101 ttgcttgcat tttcattccc cctacttttt tgcataaaaa aactcctgcc ctaagaggac
    17161 aggaggaatg tttagactga gcgtatactg atacactgat agctatacta tgtcaatgag
    17221 caatctactt atggcgcacc atacgtatcc ttgctcccac ccttcttagc ctcacgggac
    17281 taaacttaaa ggaattctat taagttttat gctttcgttg ttaattacta taccgttatc
    17341 taaattaaaa tgcaatgttt tttcttaatt tttctaaagt tttttcaaaa acttctggaa
    17401 gaggtgcttc aaagatcatc ttttcgtttg tagtcgggtg cgtaaaacct aacatttgcg
    17461 catggagaaa ctgaccattg cctttcaaag ttttcttcgg tccgtatact ggatctccag
    17521 ctaacggata accgatatac ttcatatgca cacggatctg gtgtgttctt ccggtttcta
    17581 gttttaattc aaccagagta aatccttcga aacgttccaa tacagtaaaa tgtgtgacag
    17641 ccgacttccc atcttctctt atcgcctgca ttttccggtt ttccttcgaa cggccgattg
    17701 gcgcattgat ggttcctttt tcgtgaggaa tctctccatg gactaaagca acatatttgc
    17761 gcaaagaggt cttatctttt aattgctgag ctaatgattc gtgtgcctgg tcatttttgg
    17821 caaccattag cagtccggaa gtatctttgt cgattcggtg aacaatacct ggacgaataa
    17881 cgccgttgat cgtagaaaga tccttgatat ggtacatcag cgcattgacc attgtgccat
    17941 gcgtatgacc tgctgaagga tgaacgacca ttccttgagg tttgtttatc accgctacat
    18001 cttcatcttc gtaaacgatc gtcaacggga tattttctgg gaccacatct aatgtttctg
    18061 gttcggggat caagacagta atctcgtctc cttgttttac tttgtaattt gctttgactg
    18121 gctgtccatt gacagaaacc gctccatttt tcagccacag ctgaatttgc gaacgactgt
    18181 aatcggtgaa taaattagtc aatactttat cgatcctacc agattcatgg tgaatcgtcg
    18241 ttttaagttc tgtcattttt ttctttgatc cttttcatct aaaataatat aaattaacat
    18301 acaaatcact ccacatacaa gtgtcacatc tgctacgtta aaaatcggaa agctcataaa
    18361 atctgtctgg aacatatcta ctacatagcc aagacgaata cggtcgataa aattgccaat
    18421 cgcaccagct agaatcaaac tcaatccaat catgaaccag cgattacctt tttcacgatt
    18481 tttaatcatc aatgtgagaa tcacaacgac tgcaatgagt gtcactatag taaagaacca
    18541 catcttccct tctagcatac tccatgcggc tccagtgttt cgaatatgat tcagtgacaa
    18601 aatacctggt ataagatttt tcgtctctcc aagcgctaga ttatcagtga tccaccattt
    18661 gaccaactga tccactccta ctagcaaagc gctgaatatc caataaataa ttaacaaaaa
    18721 atcaatcctt ttctatttgt ttataaatat cacgaggtcg tacgatcccg agcagattac
    18781 catcgggatt cccatcttta gtgataagta cggcttccaa agtcccttgc tcacgaaaca
    18841 tcgcttcaac ctcgtaaata tgtgttttag ctggaacaaa atgataattt gtgtactttc
    18901 cgttttggat gatcaaatcc aacgctcgtt tatttgttaa atcaaccgga cctttttggc
    18961 tttcttttgc tagccaaaac ccgatattac gtaaagtaat caatgctaca aattttcctt
    19021 tattgtataa cggaaattgt gaataccttt tttctgctac tatctttaac agttcaagta
    19081 acggaagatc ttgttcaaaa cctgttacac gttttgcaaa tcgaggcagc acggtttcag
    19141 gctgaatcag ttcttgttcg atcagctgga ttcgcttgac tgtccaatca tttggctctg
    19201 caatgacaaa atcgactgcg atccgatcat ggacgatcgc attccttaac tgagcaagct
    19261 gtaaaagatc atcttcgtat tttctgatca ttaattgttt tctttgagct aggcgtctga
    19321 caagttcggt aaatcccata tttcgtgcat ttcccatctc gtctctcatc catttttcga
    19381 ttcgattgaa actgattaaa aaaagatcag agtttgccat aattttcctc cttgatcaaa
    19441 tatgcccggt tttttatata gaaaaagaac gcaatcaaca caataactgc aaaaaaactg
    19501 aaaaataaat tttgtaggaa aacggccccc aacccagttg ctagaaataa aaacggatag
    19561 atttgaagaa tcaagtaatt cattgtgatc acataagcag gctttgcttc tttcactgta
    19621 taaacggggc tttttagcca ataaaaggtg acctgcatcg taaagggcac ttccagatca
    19681 agcgttatct tttgttcatg actgagaaca ctgcgttttt cattgttgat tcttagctga
    19741 atcgagctac ccataccgta aaaaccggtt tttcttataa atgtcgtttt catcataccc
    19801 tcctattatg ctttagtata gctcaaaata caataaagaa aaagaatgat cctagtgtaa
    19861 acaaaattaa ttccctatcg tctaaaaaaa gaagctgtag tcaaaataca gctccttcga
    19921 tggtagatta taatcaagct taaaacaatc ccaacacttc tccgtcattt gtcacgtcca
    19981 tattcaatgc agcaggtttt ttaggcaaac caggcatcgt cataacatct ccagtcaagg
    20041 ctactaaaaa tcctgcccct aattttggga tgatttcgcg gatcgtgatg gtgaaatctt
    20101 ccggtgcacc tagcagtgaa ggatcatcag aaaatgaata ctgtgttttt gccatacaaa
    20161 tcggcagttt gtcccatcca ttcttcgtaa actcttctat ttgtttttgc gcttttttgc
    20221 tgaaaacaac accatttcct ccgtaaattt tcttgacgat tgtttctgtc ttttcttgta
    20281 tcgtttcttc taggcggtat aatggcttgt aattagcttc ttttctatca atcaatcgta
    20341 ctacagcttt tgctaaatca acgccgcctt ctgctccatc tgcccataca ctcgctctct
    20401 tggcaagaat cccctgattt tcacacaagt gctccaacag tgtgagttca ttgtctgtat
    20461 ctgttacgaa ttcattgatt gcgacgatca caggtagctg atattgttcc atattacgta
    20521 tatggcgctt taaatttgca aaacctactt tcaaagcatc aagattctca tttttaagtt
    20581 catctttttt cattccgcca tgcattttca atgcacggac agtggcgaca atcaccacag
    20641 catccggcgc ttttttcaag ttaggtactt tgatatctaa aaatttctct ccgccaagat
    20701 ctgcaccaaa tcctgcctct gtgacaacgt aatctgctaa tctcaatgct gtcttggtag
    20761 ctaaaatact attgcagcca tgagcgatat tggcaaacgg tcccccgtgg acaaaagctg
    20821 gtgttccgta aattgtttgt acaagattcg gcttgattgc atctttcagc agtaatgcca
    20881 gagcgccttc tgctttcaag tctccagcag tcacaggctg acggtcaaat gtatagccga
    20941 caacgatcct tgcaagacga gcctttaaat cttcaaggtc tgccgcaaga cacaagatag
    21001 ccatgatttc gctggcaact gtgatatcaa acccatcttc tcttggaaca ccttggattg
    21061 gcccacccaa gccaacaatt actttacgca gttcacgatc attgagatcg actacccgtt
    21121 tccaaatcac tcggcgggca tcgatgttca attcatttcc ttgatgaata tgattatcta
    21181 aaagagcaga taatgcatta ttagctgttg tgatagcatg catgtcacca gtaaaatgca
    21241 agttgatatc ttccataggc aatacctgtg catatcctcc gcctgcagca ccacctttga
    21301 ttcccatcac aggaccaagt gagggttctc gcaatgcaat aacagatttt ttattgattt
    21361 tattgagcgc gtctcctaac cctacagtga tcgttgactt tccttcgcca gctggtgttg
    21421 gattgatgga agtaactaag atcaaatgcc cctcagcgtt atcctttgat cgattgattc
    21481 cagaaaaatt gattttcgcc ttatatttcc catacagttc caaatcgtct tccttaagat
    21541 ctaatttact agccacttca acaatcggtt ctagctctgt ttcttgtgaa atttgcagat
    21601 cattcttcat ttgattaccc cttttcacga actttattca acaaaatatc ttctttgtta
    21661 agatat
//
LOCUS       DACCWV010000042        20842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCWV010000042 DACCWV010000000
VERSION     DACCWV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.042"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..592)
                     /locus_tag="IVW84_001308"
     CDS             complement(<1..592)
                     /locus_tag="IVW84_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7917514.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTK"
     gene            1573..2376
                     /locus_tag="IVW84_001309"
     CDS             1573..2376
                     /locus_tag="IVW84_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7917515.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2961..3416
                     /locus_tag="IVW84_001310"
     CDS             2961..3416
                     /locus_tag="IVW84_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917516.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            3535..4593
                     /locus_tag="IVW84_001311"
     CDS             3535..4593
                     /locus_tag="IVW84_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP7917517.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            4635..6584
                     /locus_tag="IVW84_001312"
     CDS             4635..6584
                     /locus_tag="IVW84_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7917518.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            6587..10228
                     /locus_tag="IVW84_001313"
     CDS             6587..10228
                     /locus_tag="IVW84_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917519.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            10628..10966
                     /locus_tag="IVW84_001314"
     CDS             10628..10966
                     /locus_tag="IVW84_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917520.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            11076..11297
                     /locus_tag="IVW84_001315"
     CDS             11076..11297
                     /locus_tag="IVW84_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917521.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            11317..13473
                     /locus_tag="IVW84_001316"
     CDS             11317..13473
                     /locus_tag="IVW84_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917522.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDGKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            13485..13718
                     /locus_tag="IVW84_001317"
     CDS             13485..13718
                     /locus_tag="IVW84_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7917523.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            13708..14574
                     /locus_tag="IVW84_001318"
     CDS             13708..14574
                     /locus_tag="IVW84_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917524.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            14589..16559
                     /locus_tag="IVW84_001319"
     CDS             14589..16559
                     /locus_tag="IVW84_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917525.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            16726..16920
                     /locus_tag="IVW84_001320"
     CDS             16726..16920
                     /locus_tag="IVW84_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917526.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFKTIS"
     gene            16954..17289
                     /locus_tag="IVW84_001321"
     CDS             16954..17289
                     /locus_tag="IVW84_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917527.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(17546..19129)
                     /locus_tag="IVW84_001322"
     CDS             complement(17546..19129)
                     /locus_tag="IVW84_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917528.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            19251..19835
                     /locus_tag="IVW84_001323"
     CDS             19251..19835
                     /locus_tag="IVW84_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917529.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            19846..20091
                     /locus_tag="IVW84_001324"
     CDS             19846..20091
                     /locus_tag="IVW84_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917530.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            20097..20819
                     /locus_tag="IVW84_001325"
     CDS             20097..20819
                     /locus_tag="IVW84_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917531.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
BASE COUNT         7615 a         2915 c         3969 g         6343 t
ORIGIN      
        1 ctttagtgaa taattgaaaa aaactgatcg catacttgct tgtcaagtta actacttgat
       61 caaatgtata ttgcgtaaaa tgatcttcta agttcgttaa taaaggcata attgaactac
      121 taaatttaat agatactgca tcagaaaaat cattgaattc agtaatttct agcggtgaga
      181 tgactttgta ttcagtctta ccgttaggtt tagttctttg aattttaatg acagattgct
      241 gatgcaattt ttcaataact tgttttaatc ttgaatgttt tgaagaagat gaactagaag
      301 acatcaacgc aaataaagcc tttttagata ctttgacggt attattaaaa ggcggatttt
      361 tagggtcaag atgtcctata gcgtaaagat aaattttttg tggcatagca tccatttcaa
      421 caatgctatc tacaagttga ttcgataaag taacagttaa atcaaacttt tttagattgc
      481 tttcggtagt ttcaaatttc gggtttacat tcttttctgt tattttcata cgattgcctc
      541 ttctttctaa gcttggtacg agcttaaaaa cttggaactg gtacgattcc attgaataag
      601 tatagacctg ttttcgatac gttacaataa gaaatgtatc gtaaatttat aacataactc
      661 aatgtcagta aaacacattt tattttttcc aatcaaattt cattgaaaat acagcagaaa
      721 tcgctatttt agaacatttt caaaataaaa atccttttca ataaaatacg aaattataaa
      781 aattaccaaa attaaaaaac aaatattttt ctagttttta gaaataaatt cacatacttt
      841 ttcttttttt ggatatattc ggaataccac atcgcttttt gtccacgttt gcatcgcttt
      901 ttgtccacat tcacatcgct ttttgtccat gttcgcatcg ctttatgtcc accctaaaat
      961 ctatttaaga ttgatataaa gcctttttgg aggttgctaa agttttaaag attaaaatat
     1021 atattataaa tataaataaa aggggaagct gaagcttcct ccttttcctt tttttcaatt
     1081 attcaaagtt acacaagcaa aataaactcc tagatagtgt tagttttttt taagattttt
     1141 tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata tctgttaata
     1201 tatctgttaa atatctgtta atatatctgt taggggaggc ttaatctctt gggagagtaa
     1261 gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa gtggacagta
     1321 agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta tcacactagg
     1381 ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa attagattag
     1441 taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa caagcagaaa
     1501 aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa aaagaagatt
     1561 ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga tgtagattta
     1621 gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac aacagctttg
     1681 cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa aatgaaacgt
     1741 caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt caaatacgac
     1801 agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa actaggtaat
     1861 ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt tcaaaaattc
     1921 cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt tgaatttatc
     1981 ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac aaaactaaga
     2041 agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa agagtggcga
     2101 aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat accttcaagt
     2161 tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga atttttagct
     2221 cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat ctatgcaaag
     2281 aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc aaacttgcca
     2341 accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg taattttaat
     2401 acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg caagaagttt
     2461 ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct atacaaattt
     2521 taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt tcttaatttg
     2581 ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg tgatatcagt
     2641 tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt ggacaagtca
     2701 actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc gtctgataaa
     2761 tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa atagctaaaa
     2821 taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg ctgattgatt
     2881 tatttatgtt attatgatat tataataaca taaatgtgtt acgcccgaac ctcattttaa
     2941 aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt aactatatgg
     3001 agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag catgggttac
     3061 cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt attaagctga
     3121 gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat gaactaaaag
     3181 gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt attggaaaat
     3241 atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct actccaatgg
     3301 ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca atagcaaaaa
     3361 tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga agataatttt
     3421 tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg aacctagaat
     3481 taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa actaatggaa
     3541 aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg gttgcttggt
     3601 gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt tctaggagtg
     3661 ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta taattattat
     3721 ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc aggagcagag
     3781 atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt aaaactcggt
     3841 tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa cttatatatg
     3901 gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat gttaagtact
     3961 gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta tcaagttttt
     4021 attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga taccaaagtc
     4081 aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat gaatgatgtt
     4141 aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac tcctgataat
     4201 atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg aagctttctt
     4261 accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa gaataatgtt
     4321 gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa aattgaagct
     4381 acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga ttcgcaagaa
     4441 agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa tgatacacaa
     4501 aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg agacatgaag
     4561 acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat tttagaggaa
     4621 gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa actgaattta
     4681 atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt gatgacgaac
     4741 tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc ttgttaggtg
     4801 aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag cgacaagatg
     4861 gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg ggagatgatg
     4921 tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag cgaattttaa
     4981 aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat aaagttgtta
     5041 gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg ttagcaaatg
     5101 aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat atcaaaagag
     5161 atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa agcgatgttg
     5221 tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat cctcaggctt
     5281 cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta caagcaaaca
     5341 agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg gaggatttga
     5401 ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg caaatatttg
     5461 gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa gaggaattaa
     5521 agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt aaacgtatga
     5581 agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct ctgtttacag
     5641 gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga ctttatggcg
     5701 atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat gatgcaatgc
     5761 gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt attgtgtgtg
     5821 atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt ttagatgaag
     5881 gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac actgtttcat
     5941 tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc gattcgttaa
     6001 acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa tatagcactg
     6061 aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg attggtgaag
     6121 gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg gctttatccg
     6181 aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa ttagtacgaa
     6241 aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat ggtcaaaatt
     6301 tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa gaggattcta
     6361 aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa gaagcgatta
     6421 ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg agtgaagccg
     6481 gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct tccgttgctg
     6541 aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga aattcaagaa
     6601 atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag ttattggagg
     6661 agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc ctgacaatca
     6721 agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa gtaaaccggc
     6781 agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag aagttaatga
     6841 tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca gtgccgattt
     6901 cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg caggtactca
     6961 actagcatct actttagcaa cttatagtta ctacggatat attgaaacag tctcaggaaa
     7021 tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga ttggtagttt
     7081 tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg aactccttgg
     7141 aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag gtggatcgga
     7201 tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg gattaaataa
     7261 tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa tactaggtta
     7321 tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat caccagtact
     7381 tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg caggatcgct
     7441 attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata taccaacaaa
     7501 tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa atcttgctcc
     7561 atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa tcaagttcgt
     7621 tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca atactagcag
     7681 ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc tattaaaaga
     7741 atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta atgtcgccga
     7801 aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt taggggatgg
     7861 aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt ggtcagcaaa
     7921 tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc ataactttga
     7981 tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt cggtagcttt
     8041 attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata atatcgctcc
     8101 ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat ggcgtacagc
     8161 atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga tgaacgctga
     8221 atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca atgtgaatat
     8281 cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa caggatcact
     8341 tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat ctctgatcgg
     8401 actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata ctgctgtggg
     8461 attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat ctgctatgat
     8521 tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata atttgattaa
     8581 tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg ctaagttatt
     8641 atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag caatcaagaa
     8701 aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa aagatatggt
     8761 tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga acaatatggg
     8821 agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa caccagaagg
     8881 aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt tcaaagattt
     8941 agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt taggacgtat
     9001 ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag atagtaaata
     9061 cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg atgaagatgg
     9121 aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta atattggtat
     9181 ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag atggaaatct
     9241 tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata gtaacggacg
     9301 tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg atgagtttgg
     9361 taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag tcgatgagga
     9421 tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag aactcgaaca
     9481 acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg agattacttt
     9541 agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt cagacagagt
     9601 gcatcgagaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc ttaacagcaa
     9661 ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat cgctaacaaa
     9721 agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc ctaatgttaa
     9781 tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca atcaatcaat
     9841 tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat ctacttcaga
     9901 taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat cagccgttga
     9961 atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt tagcaaaagc
    10021 tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta ttaaaatagg
    10081 taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa ctggtaaagt
    10141 agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta atcaaaaatc
    10201 aactgaaaat aagtctgaag acgagtaaaa agaaggagca ttgatttgct ccttcttttt
    10261 actttgattt ctaactgaag atgtcaagga aaatttctat ttatgcaaaa aaattatttt
    10321 aaattgtcta taaatgaatt aaatatttac tgttcaaagt tgtataatta aaaagggctt
    10381 atgctggtaa agaacagaat tattatctaa acaaaaactt gataaaccag catttttagc
    10441 caataaaaaa agtgaaataa tttaatggaa aatctctttt aatcatcgaa aatttatgtt
    10501 attatgataa cataaagatg taaatatcga gacgtctttt tcttagttag atcattgaca
    10561 agtgaacaaa taagatttgt cgatgatata attaacttat taaaaatatt aaaaaggagg
    10621 tttttatatg ttcgattcta ttaaaaattt ggttagcgga atgacgcctg atatgaaaac
    10681 tttagtacaa gttgttttgt atttcttggc attcgttttt tcgttgaaac caggattcac
    10741 tgcaatgaaa ttttttggtg atgcaaaatg gggagatgga ttaaaatatg ttggggcgat
    10801 tattgcaatc tttattattc ctgtaattac aaccgttatg ctttacacaa tgggtactaa
    10861 aacaggtaat gatttgaacc aaaaagctaa tatcatatca gcacttattc cagttgttgg
    10921 tatctatcta gtttcaaaat ttccaaaaaa tgaactgtta ggttaaaagt gaattgaaca
    10981 aatttggata atatgccgat ttgttcaatt tactttttac caaaaaacta cgctccaagc
    11041 gtagtttaga agtgagggaa gaaagtaaat gtaagatggt ttttgataga gaaaaaatgt
    11101 tagctcacgc caacgaagta ttaatgagca gtctgaaagg taccgaactg gcaaagataa
    11161 tgaatatgaa tgttaaccag ttctatgatt atcgaaatgg gagtaaaaag attgaaaaag
    11221 ccagacttga aacgcttatt aaattcgaaa aagcatatgt atatatgcta gataaacaga
    11281 aaagaactat tgactgaaaa aaggagtttt acaataatgg caaaattcaa agatattacc
    11341 gaatctatta attatgtggc agaaacagtt cctgtagttg cactctcttt gaagccacaa
    11401 ttactcgaag tgtacaaaaa agaagaaaaa gaagcgaagg caaaaaaagc taaagcaaag
    11461 gtatggaaag aattcgatgc gttgctgacc aaagatttac caaagaaaaa ttatatggta
    11521 attgaagatg tacaaaagct caacagtgag ggagttatag atgaggatga tgctattctc
    11581 gattctgaga agtatgtcgt tctattgaag tcaagtttga attatgtagc tgatctacca
    11641 gaaaaattta aagagaagct attagcaggt gtccgtttta tggatagtag tggtcaatta
    11701 ttagttattc cggatgatcc taatgtgttt gaatcagaag ttgaagaagc tacagacttc
    11761 tatagcgcag tagagcaatt agaagtagct actaatggat tagaacgaat tgctgagacc
    11821 ttcaactctt ctgaagaagt tgaatttgaa gccgtagcag aaaatgtacc agatggaaaa
    11881 gaagctcttt ttgtggatga taacaaacca accgaagcag aaaaccaaga agtaagcata
    11941 gaagaaacac aaatacaaac agaaacctac caacaaccaa aaattgatga aaaacccgaa
    12001 tcccaaagtg acaaagaaaa ttatgtttat gaagctgaaa aaatcacatc tgaaagtgat
    12061 gtaccgaaaa acttagatgc tgatttttta gaaaaaaatt ttgaacagat cattgctgac
    12121 aaatttcaac ctgtagtttt agatgagatt aatgaccctg tcgatttaaa cgaggagatg
    12181 ctatccatac ctgagttgaa accattgctg tttttagcta aagagagtat gaacgctcgc
    12241 atctctattg ctaataataa aatcaacagt gaattaaata agacgaagga gcaattgatc
    12301 ctcaaaggaa agcaaatgtt agctgatgag cttttgcgga tagaggaagc aacctcatta
    12361 aatggtcaaa atcagtttac aaatacacta tcggttgcta aagagagcta cgaagcagaa
    12421 gcatctaaaa ttgatgattt agtgatggaa aaatccaata tcattcgtaa agattatgat
    12481 atggcgaaac aggcttattt ggatgctgaa ctaaagaaac tagagcaacg gtatgatgct
    12541 gagcatttac cagaagttaa acaacaagaa gatgaatata gaaaagcaac tgaagaaagt
    12601 cttgatcaac agtaccaaga agcaattgaa tctattaaaa acgatgcatt gaaacaaagc
    12661 caagaagcaa aagaagttgt ggtggaacga attatcaatt cattgaaacc agaaattgaa
    12721 aaatcattaa cagacgtgca agaaaaaact ttagagattg tcgaaaaaac aaagtcagaa
    12781 aatgaagctg agttccatga tacaaaagag aaaacaacag atgtggttgt caaatatttt
    12841 gcgaacaaag aagaagttga aaagcaggta gctaacaaca cgaaagaatt agaaacaaaa
    12901 cttgtagagc aacaaaaaac tatcgaagaa tataaagatc gaatgaaaga ggtcaaagat
    12961 ttagcagagg gttataaatt aaatagcgaa aagttggcag aacaaagtga agctttaaga
    13021 gaagacttat ctcgttcccg aagtgatcta gctcaaatgg cagaaaaaac gaaatcatta
    13081 gcagttgcta cagaaaatca atcaagttca aaacgtgatt ttacaagtaa aaagatggct
    13141 actatcattt ctgtcgctgc aatggcttgc ggaacgatct tgggatcgtc ctactttatt
    13201 agtcataata atcaacaagc acaggctcaa actcaaacat ctatttcgag agtggaaaat
    13261 tcgattggga caaatacgaa ctctgcttca acaaatagta gctatgaagc aacaccaact
    13321 acagagttca aagttgggaa agatgttgtc atcagtattg atgggaaaac aactaaggct
    13381 gaagtgattt ctattgatgg agaaaaagca gttgtgaaaa ctccagacgg aaatcagtac
    13441 aaaattcaag ctcacttatc tgataataac taaggagggc tattatgcga gaagaaaaaa
    13501 tagtttatcc agtaattcaa aacaatcaag aaaacatgac ctcttctcag cacttacagg
    13561 aagcacagct cgtgtatcag gtaaatgaac taccacaaac gaacgagaca atgagcaagt
    13621 caatatcttt aattggcttg cttattgttg caatagtgat aactgtaggt gtttaccgta
    13681 agttgaacgt gaaaggaagt atttctgatg gcagataact caaactatgg atttaaacat
    13741 aacttacatc aaaaaccttc tgaaacaatt gtcagagttg aatggcttga aaagcatgtc
    13801 aatagcacct attctgttac agcaaaaaga ttaattgtat cagctatagt tgttgcagtc
    13861 gttgttcttg gatcaattgt ttacttagtt tctgatacga ttaaactgaa atttattttt
    13921 gttccaacgt tattaatgat aattggattt cttattctaa ttagccagta tatgtttatt
    13981 gatccatcta gtggttcatt gatttctggt tctattagac tgttcaaatc agtagttaat
    14041 tcagcaagag tatctatggg gaaaaagaaa ccatttcgag gcacagagat tttaaaagtg
    14101 aaacaagatg gaacaatcgt atatcgagat gggagtgtag cattaatgtt caatctagat
    14161 ggggctacat cactaaccgc ttttccagaa gaagttatgg ctcaggaatc gaaatcaata
    14221 caatatcaaa ataccagaga tcgttcctta gctgaaatca aaatcacttc atctcaacct
    14281 cagaatacgg aaagacaaac tcgaaatatg aaaatacaaa aacgaaatgc aaggagtaac
    14341 aaagcagtct ttgacataat agatcagcaa gaacgatttg tatcagaata tattgatggt
    14401 gtgaaaacaa cagtaatcca atatcttatc ttgaaatctc aatctaagat ggtattggat
    14461 gaaggtgtgg aacgtttaga aaactatacg agtagaggac tttattatag tgtcactcag
    14521 ttgtcagcta aacaaacaaa agaaatatta tctgatattt atgcactgaa atagaggtga
    14581 gagattaaat ggaaattatg ggtagatcac aactattaaa tttcaaaaaa aagagaaaaa
    14641 ctaaaaaaga acaaaaaact agaaattcaa aggtagtgaa taataccttt gttagtgatt
    14701 taacatcttt agttggttat caatttacac catcttacat taggacaggt aagcggtggg
    14761 gaacaattgt gaaattagtg aatacttatg gaatgaatag aaattatgga tttggttgga
    14821 tggttaaagc tattcctgaa gtgagagtag aaggggtaaa agcctatcta attgaagcag
    14881 ataagccagt gtcacaaaaa gatcaacaag aaataatgca cgagaatgtt gggggatcaa
    14941 cgaatagtta tgaaaataat ttagaaggaa aaaattcaga tgctgaaatt agcaaaactg
    15001 ttatgcggat tatggatttg aatacagcat ctttactaga cagcatggca aataaaatta
    15061 ttgattggaa gctagaaatc ctattagtat ccgattctcc taataaaatc agcgaacagc
    15121 taattaaact ccaacggttg tatgatgaaa aaatgtatgg attaaagctt atgtcatatg
    15181 gaggagatca acgtgatctg tttgatagac ttttaagtga accaatcggg aatgtctatg
    15241 agtatacgtc tatgagttcg gattttgcag gaaatgatca tgcaattcgc aaaggtttag
    15301 atgatgcgaa tggatgggct atcggcacta tgtctactag ctacgcaagt ggtgtagctt
    15361 ttatggcact ggatgattct tttacaagta aagttttagt ggcatcacac aaaaacgcta
    15421 caattaaagg ctatgatgaa aaaatctcag catcatcact ttgggggcaa ttgattgcta
    15481 ataatgcaat ggcaaatcat aaacgaactt ttcatattgt cttaaataaa tttgattact
    15541 ttggtgatcc aactggtaga acatttgttg ccaaaccttc gttaaacagt gtaatagaaa
    15601 aggttaattt ggctgaagaa ggtcttaact ctttggagct ttttggtaat caaaaggaca
    15661 ttggtgattt atatagacgg aatctcgata aactaactca gattttttat ttgttagcag
    15721 gcagaacatt agattctgca actagagttt tattgaaaaa agaattaggt aacttttata
    15781 taagtcagaa actttgggat attaattatg aagaacgttc aaaacttttg gtgaacagta
    15841 gtgatacaat gccagttctt ggggatttta ttagctattt gactaatgct gaaacaaaag
    15901 ccaaaaaatc tggtactgaa aaagaagtcg atcgtattat tgtattaaaa gacacattga
    15961 gaacagcatt agacagcttc tctaggatgt ttaatcaaag aacgacactg aaagaaatcg
    16021 gtaaaagtga caaattacag ttttactata acttaggtga tctaagagca gaaaccaatg
    16081 ttatggaagc tcaattttta aatacctttg atttcatcac atcacaagca aaaaaagatg
    16141 atattgttat gattcacggc ttcgataagt tatctattga aaccgttggg ctaattaaaa
    16201 aaagattaac aatgcttgaa gaagatggaa ttcgtcttgc ctttctattt gatacaatcg
    16261 gttcaggaga agacaatgaa tacaaagaag atattgaaac agctgatata ttcaatacag
    16321 atggaaaaat ttaccaaagt tttgaaagtg attttaatta cactatcatt ggaaagatga
    16381 ccagtggaga gttaatgaag tatgaaaaaa aagttgctcc tgctagaaaa ggagaagatg
    16441 atcaaaaact ttctgttgat ttgagacagt atttgacaac aggaagaagt aataaatttc
    16501 agatcaggag accagtggat ctgactaata attttgttat tggagagttt attctttaag
    16561 ttttatcttt gattttgagt gtataaggaa aggataagaa aaaacatgta tatttgagac
    16621 taaaaacagt cttagagaac atggtacgtt ttgtgaaaag agaactatag aaatttcata
    16681 aagtgaaacc ttactgagtg aaataattat agtggaggtt aaataatgaa accaaacata
    16741 aaaattttag atcgaatatt tctaggtaga gacacagaag taatcctaat tcaacatgaa
    16801 gaaggatttg aagttagtat aggtattcag aaacttcaaa aacctcatta ttgtaatcag
    16861 ttgtataaaa actttactga tgaagaaaaa gcgagagttt tttttaaaac aatttcctag
    16921 gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa tgaatggaaa
    16981 gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg cttagagaag
    17041 gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc agaccaattt
    17101 ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa tgatggaaaa
    17161 tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac atgggaaatg
    17221 agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat ttcacacacc
    17281 gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat ccttttttca
    17341 atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta tatcaaaata
    17401 aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt atatttatta
    17461 ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat atacgctata
    17521 aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt aacaattctt
    17581 tctcggaatt gatttctttt cttaaatagt cttcagactt attaataata ttatctatta
    17641 tgtctagata catatcactt ttcgattcag ctaattcata cagttttttt aattctttat
    17701 cctttaattg aaaaccctga tccatagtca ctgcatttgg atatagaagc ccactataat
    17761 gtttacgtaa attcacttgg aatttctgat tataaaagtc taacagatta gaaataaaag
    17821 gcggaaaatt actgtttttg taaaattgat tatttccatt taggttgtat agtaatttgt
    17881 taaattgctc ctgatttaat ttattgtaaa tccatggtaa tacttccata tccttatctg
    17941 ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg aacatctctt
    18001 tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat ggtagcgaag
    18061 aaactctcat tttttgctgg agtataggat caactagact atttaagtga ttttgtaatt
    18121 cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat tctatttctg
    18181 ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt tgaattgtct
    18241 cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt aaaaagaacc
    18301 cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc tctcttgatt
    18361 cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag aattctttat
    18421 tatcaaaaat tagggcatta agtagtctga aagtactttc cctatctcta tctgcttcct
    18481 catttccata agataattga aaaacaaact taaaggctgt cttacatagg ttttctttgg
    18541 ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat tcttcaggtg
    18601 taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt actttttttc
    18661 ctaatattgg attttttagg aatataattt cataggactt ccgtataaca tcctgacaag
    18721 tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact acaggatgag
    18781 ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag ctatcaatta
    18841 gctcagttga actataagct ttttctagta gttccatttc ccattttgga gagtttggat
    18901 cactttttct tagtctagct atatcttcta aaattttctt agaagtatta tcctcgtaaa
    18961 tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg taattgacag
    19021 ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct aataatggaa
    19081 taattctagg atttttaact ttttttattt catcatctaa tataaacata taaatctcct
    19141 ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga catatttatg
    19201 ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa atggatttga
    19261 aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg gctgatattt
    19321 ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat ttaatcgggt
    19381 acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt gctaacaatc
    19441 aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat gtgatccatg
    19501 aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca atgacaacgg
    19561 atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg cgtgatggtc
    19621 aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa atttcttttt
    19681 gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt aaaacagcct
    19741 ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac ccatataatt
    19801 ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa aaaaattata
    19861 aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat gtattttact
    19921 gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct attatttagt
    19981 ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt gttttattgg
    20041 atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg aataaaatga
    20101 aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc cttacagcat
    20161 gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt acgatggtta
    20221 ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa aataatgctg
    20281 aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa gtttataaca
    20341 actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg caaaataaaa
    20401 atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca gaatcaaaaa
    20461 tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat gatattacca
    20521 taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat ggtgtacctg
    20581 agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat acgccaacag
    20641 ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta atgggaatga
    20701 caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg gatttgggag
    20761 catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa gttaaataga
    20821 tctttagggc gtaagcccta tt
//
LOCUS       DACCWV010000043        20364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCWV010000043 DACCWV010000000
VERSION     DACCWV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.043"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(117..1001)
                     /locus_tag="IVW84_001326"
     CDS             complement(117..1001)
                     /locus_tag="IVW84_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7917532.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1096..1533)
                     /locus_tag="IVW84_001327"
     CDS             complement(1096..1533)
                     /locus_tag="IVW84_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917533.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(1750..2661)
                     /locus_tag="IVW84_001328"
     CDS             complement(1750..2661)
                     /locus_tag="IVW84_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7917534.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3038..3676)
                     /locus_tag="IVW84_001329"
     CDS             complement(3038..3676)
                     /locus_tag="IVW84_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917535.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(3680..4450)
                     /locus_tag="IVW84_001330"
     CDS             complement(3680..4450)
                     /locus_tag="IVW84_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7917536.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(4868..5284)
                     /locus_tag="IVW84_001331"
     CDS             complement(4868..5284)
                     /locus_tag="IVW84_001331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917537.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(5335..5716)
                     /locus_tag="IVW84_001332"
                     /pseudo
     CDS             complement(5335..5716)
                     /locus_tag="IVW84_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5842..6507
                     /locus_tag="IVW84_001333"
     CDS             5842..6507
                     /locus_tag="IVW84_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917538.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(6904..8937)
                     /gene="brxL"
                     /locus_tag="IVW84_001334"
     CDS             complement(6904..8937)
                     /gene="brxL"
                     /locus_tag="IVW84_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAP7917539.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(9055..11577)
                     /gene="pglZ"
                     /locus_tag="IVW84_001335"
     CDS             complement(9055..11577)
                     /gene="pglZ"
                     /locus_tag="IVW84_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAP7917540.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(11693..15325)
                     /gene="pglX"
                     /locus_tag="IVW84_001336"
     CDS             complement(11693..15325)
                     /gene="pglX"
                     /locus_tag="IVW84_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HAP7917541.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(15389..19060)
                     /gene="brxC"
                     /locus_tag="IVW84_001337"
     CDS             complement(15389..19060)
                     /gene="brxC"
                     /locus_tag="IVW84_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAP7917542.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(19085..19660)
                     /locus_tag="IVW84_001338"
     CDS             complement(19085..19660)
                     /locus_tag="IVW84_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HAP7917543.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(19660..20259)
                     /locus_tag="IVW84_001339"
     CDS             complement(19660..20259)
                     /locus_tag="IVW84_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAP7917544.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6306 a         3901 c         2866 g         7291 t
ORIGIN      
        1 tgttaggtga aaatttcttt tttgtacaaa aaaaggctgt gacaaaagtc ttgtcacagt
       61 cccttattcc gaacaaccga tgtcctcctg acaactcttc ggtcacaacc tccattttaa
      121 caataaacct gtaaatgtga gggtaacact ttgattgtca caggtaattt atccccttca
      181 tctccatcaa cattcgtcgt caattcttcc tcatcagcta atgacacaac taattgctca
      241 caagtaagat aggctagatt tttcgtagat tcatctactc cttttaatag gtctggcaat
      301 gttttaagcg tatcgatcaa agaagaatct tttaaataaa ccaaatggag cttaccatca
      361 tccacttttg catttggaag taatgtttca aaaccgccta ctgagttagt tagtcctatc
      421 agcaagatac tgctctcaat ttcttccttc tttccatcaa cttctacgtg aaacgagtag
      481 gattgtgtgg aagctaactg tttgattcct gacatgaaat aagctagttt tccaaatttc
      541 gtcttcttct ccgcatctac atcattgatt gcttcaggga tagagccaat tgcgacgaca
      601 ttcataaaat agtcgtcatt tatttttcca atatccaatg gcttgactga ttcaatcgaa
      661 aaatgattga tcgcttcctc aggttctagc ggtatcccta atgctcgagc caagtcattc
      721 accgtaccca atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc gattccactg
      781 attccttcgt tgactgttcc atctccgccc atgacaaaca cactatgata cccttccgta
      841 gccgcttcgc gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa gacgaccact
      901 tcatcaaaca ccgattccaa ttttgctatc gctaattgct caaattcctt tgcttgttca
      961 ccgcctgaac tggggtttac cactaataaa acttttttca tcactttctt tcccttctga
     1021 ttgatttctt taatataacg atagcataaa gaaaaaaatt aggctgtgat gaagtactgt
     1081 tcatgaaaca ttcatttatt ttttgacaag ataaaatacc catactatga aactgaggaa
     1141 aattaaaaca atgatatagg aaataaaaga aaatgatagg cattgtatac ctaataaagt
     1201 aaaaatctta ggtggcagat aaaaacacaa aaatagcaag agaatgtaat agcgtttctt
     1261 ccactttttg ttatcagacc ttatagcgat cagaggaata ccaaatgcat aactgaaaaa
     1321 ctcttgatgt actgttaata tgtcacgacc ttgaataata aatgcaacaa tccccgctat
     1381 aattagtgtg cctaatacca aaacggaaaa ttgatgaata tattttatat acaaatgatc
     1441 cattgtactt cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt gaataacttc
     1501 tagaaataaa agaaatttgc aaaaccactc catttatcta cttctacgtg cattttttaa
     1561 tatcgttttc tttatttaag attatacaga aaaatatcta gggtggctgg attctaagta
     1621 aaaaaaattt ttaaaataaa aagaactaaa tcaagtaata attaaactac tgttgattta
     1681 gttctaataa aaagatttag atgaacagtt ttattgattt aaatagttca tattttttat
     1741 ttataccgct tatttactac ttgctgtact agaatctgaa gtgaatgtac tcaaaacatt
     1801 ttcaaagtct ttgtctttga tttttacatt agcgtctttc aatacttcgc caattacttt
     1861 attctggaag gtactgtcac ttaattttgt gtctgttgca atttctttca attctttttt
     1921 gtatttatcc atgtcgttgc ctttgttttg gtttttcacc attttaacca cataatattc
     1981 cgtagtataa gttgaagcat tggtagaagt gatcacatca gaaacttgac catctttcaa
     2041 cttaaaggct gcttctttga cttctgccgg aaccgttgta gtagtggaat caaatttcac
     2101 ttttccgcca tcttcttttg tcgtagtatc agttgacttg tctttggcta atttagagaa
     2161 atcgtctccc ttatcagcag ctttcttcac atcttttgca tcatcttcac ttgctacttg
     2221 gataatttga gcttccactt ctggatggaa agattcccaa gccgttttta aatcttcatc
     2281 ggtcaatttg atatgtttct ttagaccttc ttgaaaggct agactttgtt tgatactgtc
     2341 tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg aatcacccaa
     2401 ttgtttcttt gtttgatcat attgggaatc gatttctttg gttgatacgt catctccata
     2461 tttatcttca aatacttgat aaacgatcaa ctgttgcaca gcggaatctg ctccttgcat
     2521 ttttttcaag ctatccatta cttcttgttc gctgattgta ccgcctttat aactaacggc
     2581 tgttttttca ccggcattcg tacagcctga caataagcca gtgcctagta cgatggcaag
     2641 tgctaaaaat ctttttttca tttatcaatt ctcctacttt tcgttttttg cgaattgcta
     2701 aaaacaaagg tgttcaagat gaaaatcatc aagaacacct ttactaatct gctcgatacg
     2761 tcctgaatag atataaggct acacaatcca gaaagttcgc tcagcagaat aaatgtcaag
     2821 tagcacatca caccttaata tactacttct tttgttaccg tttttcaagt ctatcttgcg
     2881 ttccgtattt aacgtaacta attcttagaa caaatgttat cgattttgtt tttcttcttc
     2941 cgctgttggg atattcaaat cagacacttt tatgttcgat tttccagaca tttttttgtt
     3001 gtttttatta gaaaaagttt gttttttcat ttgttttcta aagcatgatc ttcttgtttc
     3061 ctattttggt cagatatgga tcatgcgtga ccacgataac taacgtacct tgggaagcct
     3121 gttgcgataa gatttcaacg acttgctcct tatttcgttt atctaatgat ccagttggtt
     3181 catcggctaa aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct
     3241 tctcaccacc agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt
     3301 tcaatgcctc atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc
     3361 caatatctaa attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa
     3421 aagagagggc atttcttttt atcaagcgct tctctcttcc agttaaatgt tttgtttctt
     3481 gattaaataa ataaaattgc cctgaactga attgatcatt caatcctaat atattcaata
     3541 aagtactttt ccctgctccg ctctctccaa ctacagtcac aaaatcccca gcatccgcag
     3601 tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca
     3661 ctatcaacgg ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg
     3721 tagacaaaat aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa
     3781 aaagatattt aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt
     3841 tgatgaggtt catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac
     3901 gaaagatagc gattttccga caattgtaga caaaatatat gcataacatg acatagctca
     3961 acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc
     4021 ttaacacttt gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat
     4081 cacgtaactg actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct
     4141 tgatctttaa cggtggatag atttctccgc tgagaatatt tctttcccaa tacgtactgt
     4201 ttttcaatgt cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa
     4261 atgtataaat gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt
     4321 cttgataata ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt
     4381 cagggatcaa tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga
     4441 catcaatcaa aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact
     4501 gttcattttc ttcattcaag taatctgata cattgactaa tatacttccg tttttattca
     4561 aagtttcata taattcctga tcggctgctg atgcaaaacg attggaataa ataaaatcca
     4621 cgtggttttt cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt
     4681 ttggtgtttc atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga
     4741 cattggcaac ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga
     4801 tatagttgaa cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag
     4861 agatacccta tattccgctg aacaagaaaa cgtacatctg gaacaaataa agaaattgct
     4921 ccttataata cataaaaata aaaccaccaa tgattcctgc aattatgaat gtaataatga
     4981 ttttcttttt ccccaataaa caccacaata cttgatgtaa tgaaaagcca ttattcaaca
     5041 ataaactgat ttctttattc atcaataaga aaaaggatga taacaaaagg aataaaagaa
     5101 tcaatccaat agttaaatac actcgataat tatatacgtc tgtacttgaa ataaccgaat
     5161 acgaatagct attttcaaag tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt
     5221 gattgaatcg ttccacaaat agtaaaatga accgttcata ggcttctttt tctctcgtca
     5281 tcaagaaaaa ttcaccttca aaatctttta ttttgatcga ttcttcaatg gacgttacgc
     5341 gaaaggaggt attagaatag ggtaccttct cgtgagaaga tttgacatcc ccttgataat
     5401 acaatgtcat ttcaagcttt ggctgtatct tgaagttcca agatgtatct acttcattgg
     5461 attcatttac tgtttttttc atataagaaa gctcagtttc tgatgagaca ttttcaaata
     5521 ccatgattag ctgctcatat tcttcttgcc caataaaatg ttgtggcaat cgaatcggag
     5581 tataagtatc tgtttgatgg aaaaattcat ttgtccgata gagtacctgt ttatttataa
     5641 taaccaaggc aaaaccactc atgataccgg tcactatgat tgtgagtaaa actgtgaact
     5701 tttttttgat tatcaccact ctcccttact taaatgattc tttgataaaa tcataccgtt
     5761 tttcatcaaa aaaacgtatc tatcaaatgt ataggtcatg tagaataaat ctatatactg
     5821 tagaatcgaa aggagaagaa gatgaacatc ggagaaaaaa tacgaactta ccgcagaaat
     5881 tgtaagctta cgcaaaaaga attaggcgaa aaaatcggtg tttccgataa aacaatctct
     5941 agttgggaaa attctcgtac aatgccagac cttgaaatgc tgtcactttt acatcagact
     6001 ttaggtctgc cgattgattt tccttctatt gaatcagcta ccgaatcaat agaaccaaca
     6061 ttgccggatg aacctcttgt tatcaagaaa tcttattcat caaaacaacc cttctcactc
     6121 aaaaagacac tatctttctt tctgctcgtc ttttgtttgg tctatctttt tcttatcaat
     6181 tatcaatata aaaatgaaac ggttgatcga ttcaatcctt ttttgaaacc acaaatcggc
     6241 tataccacac tccctgtagg atacgtggaa aattccatcg aacattgggt atgtaatgat
     6301 atctttgttc atggtacatg gctgtctttt cacgctggag aggtagaaaa aggtaagaat
     6361 catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa
     6421 gccattccct cagattacca agaaatcctc atccaagact atgacgatgc cttaaaaaga
     6481 agccctcagg agccagatag gcattaagta ggagatgaac gtagagatag aaaaaatcaa
     6541 tgcacatgat cttatcaaaa aaaaacaggg gtacaaatta attgaaaaag ctttgagatg
     6601 aaagttccta aataaaaaag ttcagaatga taggtaaaaa aatgactaac tattctagta
     6661 ttaggctatt tgtattattc ttctgttatt tttatatcaa aaaatccaga aataactaaa
     6721 tataggtatt tctggcttat agtataatat ttacgccaca gcccccaatg attctttttg
     6781 gaatcatgat caaggacgga catacacaaa aatattttgc ccattcgatc cgaatagatt
     6841 aacgactaat ctagtaagta atatagattg gtacgtagtc aaaccagcaa atggaaaagt
     6901 tgtctattcc accccaaggg ctttaaagac agcatcttcc ggcgtttgat aaaaaatcaa
     6961 attaaaggta cccattaatt ctgggggtac agttccaata tcagcagcgc ttatcatagg
     7021 tacaagtact tttttcgctc cggcatcttt tgctacttgt aaacagttag ctaaattatc
     7081 taccttaatc atagaaccac caattgtaat atcccctaaa ataactaaac ttggtaatac
     7141 aggtttattt aaggaaactg agcataaagc tattatagtt gataacgata atgtttttgt
     7201 cataccaatg ccattcatat cttgaacatg aacgacaaaa tcttttgtcg tcaaactaat
     7261 agactgacta acacttccat aattagctct taaatatcgg tatgccgaat ccatcgcttc
     7321 tcttgctcca gcatctgaat tgatcccagt acgttcaaac tttccatttc ccgaagtcat
     7381 ttgcgtttcc aatttatagg tacctaattg cccagttttg ccataagata ccgtgtacaa
     7441 atgccctggc ttccctaacc cttctggaat caaagttcct ccaccttgtt caggaacaga
     7501 aacatactct tctcgctctt cttctaaatc aatgtatgaa aaattgacat cataaaattc
     7561 cattccacca attttcttta attgttcttt tacacgacga cgcatttcaa gagcaaatct
     7621 tagtatttct tcgacatcac ttttttcaaa tctaccatca ggatacaaca gtttcatcat
     7681 cccagataca atccgacgaa ctgcaatcgt atcccgctga tttaattgag aaccaagttt
     7741 gaaatattta tcaaaactat cgccaaaact aacttttcga agctcacgca tgacttcaga
     7801 aaaataatct gtaatgaagc catagtcatt tgtaaaaaaa tctggtcgat atttaggaat
     7861 ttcccaacct ggtaaataac aatgcatccg atccaaaaag gctgaatccg tccccataac
     7921 ctccggaaat ggctcaaata aattcgaagt tttcaatatt acatcaacgc tttgattaat
     7981 atttcctaca aaagccattg atgctgaagc attcttttct tctttgcctc tagcaaaaga
     8041 tcctgaagcc atgtaatctt tcatgatttg aacgccatca ttatccttaa atttaatccc
     8101 agcaacttcg tcaaaagcaa cacagtccca catacctaca agtcccattg tacgactagc
     8161 catattatag aaaaggtttg ctactgttgt ttgtccacct gaaacaagga ttgagtttgg
     8221 tgaaatttct ttatatacat gcgatttacc tgtaccacgt ggtcctagtt cacagagatt
     8281 aaaattattt tcaattaatg gaataagtct tgctaaatga agccatttta cccttttatt
     8341 aaattgtgta ggttccatac caatggaccg tagaagtaag tcaatccatt catttgtagt
     8401 aaactccttg cgagccatct tgtaagcctc aatgtctaaa ccaggcattt ggattggatc
     8461 aacctttttt gcaataaaag ggctattcca acgatcattc tcatcatacc aatattccat
     8521 cgaaacaata caccagatcc ccccacctaa taatcgatca aattttttcg gatactcttc
     8581 actaatttga acatttttta atcccaggtt agagaattct gcttcataac aatctttctt
     8641 ttcatttaaa tgtacagtta ctttatcaat aactgtataa ccacccaatt cacgaatttt
     8701 tgcttttact ttttctgctt catcaggacg cacatagttt cgagcaagaa tatttttaac
     8761 attttccaca cctttttcaa ccaatgtttc atccaatgac gaagcataca taccaagtaa
     8821 atattccaat acataaacag gtacattcgc accttcttta attttctttg ttaaatcttt
     8881 tctgacaatc tttccgggaa agaattcaga taactttaaa atttcctgtt gttccactag
     8941 aattcctcca tgtgttcagt cattataata gcaattaaag tatatcatac ttaaccagta
     9001 ggcttgataa tacaaaagaa aaagtatcca caaaaaattg tggatacctc gtttttatat
     9061 atcaaaatca aatgattctg caaaaggatt atctataatg aattttattc gctcggtcaa
     9121 atcatttttt tgatttttta tcactaaata ataagtatct tcaactgtat atcgacgatt
     9181 tacaaattca aaaacaaatt tagtaaaccg ctgatttgca tcttctgttt ttgaatccgc
     9241 aacatagatt tcttcatttg agatacgttc accatattca tcttcaaaat aaagccagaa
     9301 agatgcgggg tcataaacat cactaattgc atttttttga tagaattcta acgtggttga
     9361 taatcctgta attctgcggt tgctagtcat taattcaatt gctactggtt cctttgcatt
     9421 gctacctttg gctaccgtaa tttctagaac agggactagc atttcttgaa tagatgaccc
     9481 tccatgaacg tagctttgtc ccccgccagc tttcttaaaa atacttgaag taatcgggac
     9541 acaaatgttt ctagtgtcat catttcctaa agaatctccc aaagagtaat ttttcgtacc
     9601 aattatttcg tattcttctt cactaagaat aaaacgacgt tctacacgat ctttttcatt
     9661 tctagatgga ttttcaatct tatctacttc ttcaacgtgg cttcttttat aaataaatcc
     9721 atgatcagcc gtaactataa aacgatagat actgccatca ttatgcaagc gattaataag
     9781 tcgggaaatt tcgcttatcg ctaaatcaca ggcctcaaaa actcgatttt cattcgagtg
     9841 atcacctgtt ctatccactt gatcgtgata aatataaatg actgatttgc cattaaatac
     9901 ttctcgtacc tcagccttat tcatcttaac cacatcatca taatttaaag ctacagcatt
     9961 tgcatctcgt tgctggagaa tcgcatttcg attggataaa ccattcgttt ttttcccatc
    10021 aatcagtact tctgttcctt cattgtaagt tagttgattg tttggtaata aggcggcttt
    10081 accgaattca gtaactgaag gtaatacaga aagaaagcta gccatgtttg ttgaatactt
    10141 cttttctcta actaacttac gttctaaatt catccctaac tcatatcgaa aagcatctga
    10201 aataataact acagttttaa cagagcgttt cattacagct tgattataaa attttcgaat
    10261 ggatggacga ttcttaaatt caaaagtttg attccaaatt tgtcctgatt gatcaagaaa
    10321 cttcgaataa tttgcttcaa ctaatcgata caattggcca tatttttgct tttcttctat
    10381 atgatcacta ttccaaataa atttccgata atgcgtatca acccaataca tttccttctc
    10441 atattgagtt agcaaattat ccatgttcat cggcaagcac aatctgtttt tatccacaag
    10501 ataataagca tgtttcaata attgatactc ataaatataa tccgatgcga aatgcatttt
    10561 ttcgcgctgc tttaaactat caaaaattgt ttgtcctgca attttcgttg tagtgtcaga
    10621 agcctttaat cgttctgcat aaaacattaa tatttttgca tgaatctcag aaaatacgtc
    10681 tgcttcaatt agatcatcaa tttctatact gtctaataat ccatcaccat caatagcctg
    10741 atatactttt ttagatagct gttcaaagct ttccatgtaa cgtgtatcat tcataaactg
    10801 atccatgaaa gtaataatgt ttgctttcaa atgtagtatt tcaaatcctt tgaacttctt
    10861 cggcatctct tgatcaattt gataataaaa atagttcaaa taaagacaca tcgtcagctt
    10921 ttccaatgtt ggctggctat ccgtaaagcc aaatttcatt tgaataaatt cccaaaattt
    10981 agctgttaac tgatacttat caagttccct taaaggattc tccctacttt ctgtaaagct
    11041 ttttagaact acacgcagta aatcatttaa tcgaatactt ttcgatttca acaaaacagc
    11101 cattatcatt aattcaatcg tttctgattt attaattgtc atatgcgtac taaattcacc
    11161 aaaacttctc atgcgatcct ttgcattaaa gaaattacga tactgtttaa ctgtctgatg
    11221 aaatctaagt ggaatagcca attcattcat gataatcgct aagcggtctg cactaaattc
    11281 ctctgaataa ttcaatacac tcaacaaatg attatcttca tctttatcat cccaacgagg
    11341 aaatggcgca tagattaaat agttagtttc gggatcttca tattccaata aacgcttagt
    11401 tttaaattgt gcgtcctttt ctaatcgata gatttttgca ttttgaatga ccaatgagtc
    11461 aatttcttca agaaaatcct gatttgaatc aaaccaaaaa atgattttcc tacatccata
    11521 attaggaaaa cgttgattta atttttcttc aatttgttgc gtatttatat ctgtcatcaa
    11581 tctcacctaa tcgttctctt tctatacccc ttatattgac ataatttgga taatttcgca
    11641 cgcaatttaa gaataaaaaa gaaagttgtt agaaaacaac tttcttagat aactattttt
    11701 ttggttcttt gacccctttt gcaaaaattt ggaatttctc gccggtttta ggatcacgct
    11761 gcaacttatc ataattgaca attacaccat catctaagtc taattcaatt cgttttaatg
    11821 ctaagtgacc caacttctcg tcatactcac gaacttcttt taattgcttc tgaatcttta
    11881 caatttcttt tgtcattgca tttctttctt ttgggttatc acttgttgaa atttgttgct
    11941 gacgcaattc gattcgattg tcatacgctt tttgtagttc gtgtaggtat tcagtacgta
    12001 cttttcctaa ttgatctggt gtatagcgat gcaagtacat taacgcttta aagccatttt
    12061 gttttcctga gtccaattgc caataaattg gtcgattgga gtatgttttt aaatggtctt
    12121 tgtaaaaatc ttgtacgaag taacgacgaa taatttctcg acttgtccct ttgccaccta
    12181 aagtgtcagc gataaactgg agattttctt caagggtttc tttaccaaaa gtaatctcaa
    12241 cgaaatcaat gaaacgattg acaatgtcaa tttggctatc cttgaaatac tcatcatcgg
    12301 tgatgagtaa aacattatca gtatcaggtt taaatttttg atagcgggaa ggatcccatt
    12361 cgccgccagc gaagatgaga ccatcttcgt ctagggagta acgaccgaag atacaaccta
    12421 ttgaataaga aataaaatcc ttaatttccc tcgccttatc aggtaacctt gcagagatat
    12481 attcatcttg aacctctttt gttaattctt tttcaagaga ataatgatta atgaatatgt
    12541 cgttcagttc ctcttcattc gattttagag tattaaattg attaaccaac tgcctactta
    12601 gacagttaaa tgcctctgag agatcttttt taaacatata acttaacaaa atgtttcttc
    12661 taaactcgta tgacgtttca agagcattat agtcttttct agctatatcg atattttcat
    12721 ccactttttc tcttattttt tttaattttc tctcagatgt aactttaaga aaaggtagtt
    12781 ttcccattgg tccttcatgg taatccaagg tgggagaaat aaaagttaaa aagtgattaa
    12841 ctaatttaga gtttacatag ccaaatattt cttcaaagtc agttatatcc tttgagaaca
    12901 gaacagatcc tttagtttca aaaattgcct gattctgcaa tctaaaagat acttgtccag
    12961 atgaaagtgt cgaccaagta attccctttt taaaatagta attaagattt tgtggtctag
    13021 attttaactt cccattttca ttaacataat tttttatttc atctccatca ttctcccagt
    13081 taaccacgta ttcattgttt ccataccatt ttctaaaacg accaccttta gttgtaggaa
    13141 accatttttt tctagacttt ttagcgtcct ctaatgtttt agcatcaaac tttattttgt
    13201 taaattctac ttcaaaccaa taccgcaaaa atttattgtt atttccggtt gccaaccctt
    13261 gttttgcatc tgcaacatcg ttagttaatt ttccttgagc aaaatcagtg attacgttat
    13321 cgcttgccca gtaagcaatc ggcattcctg ggattttttt gaagttgtct tgattagtac
    13381 tataaaaata atcgcattca ccatgctgat gaattgcttc taacgtttta attttttgaa
    13441 cttccattcc acctttaaaa tctgataatt ttatatagct tccatcttgt tcatttgaat
    13501 tttttatcgt aaacgtacaa attggaacgg ttgcttcttc aaaagctgaa tactccattt
    13561 gaatcaaact agaaatgttt ctttcattaa ttaaaaatga gcgtaacttt tcatagctag
    13621 aaatgaacat ccaaacaaat ggcgtcataa aggaagcata tccattatca atcagcattt
    13681 ggctattcaa tttaataaac acagaaaata gatctttttt gacatcagaa tagttatcat
    13741 ttacatattt ttttaatttc ttatccattt tattaagata aggcggatta gtgactaccg
    13801 ctacatattt atcaactagc atttcagcca cgtcaagaat gtgcaagtat ttttcttgta
    13861 tttccttcat cccataaaga atatccatat tcgtaaattc gttgaatttt gatatatact
    13921 ctcttacttt tcctaagtat tctttagtta ttgcctgatt atttttatcg actaacttaa
    13981 tgattgatcc aagttctttc ccattttcaa attgatcaaa aattaactct ttgtattcat
    14041 tccagcgttc actatactta tcattgttca attcactaat acgctcttga tactcgtctg
    14101 tcactttagg agaatcaata aactctctta aattcggcga tagacttttt ttcaaaaaaa
    14161 gacgatcgta ttgacgccct ttcatcataa ttgcaaaata agacaattga tacgctcttt
    14221 tatcgatttc aagaccatat aaattgtatt caacgattgc acgtgcagca tcacgctcag
    14281 aatatcctaa cgtaagataa atttccatca atacatcgaa ggcgtaaact aaaatatgcc
    14341 cagaccccat tgcgttatcg ataattttta gttcttctgg ttttaaatta cttacagatg
    14401 ttgcttgttc aaaagcatat ttatctggaa ttaagtattt caacttattt cttaattcac
    14461 tattgggata atgatctaac caatactttc ctaaactatt gtcaaccata taacgaacga
    14521 cccaatctgt ggtaaatagt tgagttgctg ccggaatatc ttgttttttg acaatccctt
    14581 tataaatatt caccacttga ttatgtggct cactattata gtactgatac aaccacccaa
    14641 taatctctac ctgtccacca gcttgaacat agaaatcatc ctctggaata cttaaaagat
    14701 cagcgattac accatctttt ttaatataag aagctgtaaa caaaagttct gtataatctt
    14761 ctacttcttc aaatagctca ggtaagtaat cattcaattg attacagatt tttataaaaa
    14821 tcaattgata cagcttgtcc attgcttctg ctgaattatc tgttttccaa gttctgactt
    14881 gttgctgttc attactagat aattcttcaa agatatcggc atcataaata tctgtaataa
    14941 tatctggctc aattttccca cttatttcac tagataatac acgatacgtg tcaggtaaat
    15001 aatcatttac ctccataaac cgaatggcaa tcaatcggtt aaaccacgta taagcaacct
    15061 cttcgattaa ctcttgaaaa gcttctgaat aagaagtgac agaagcacgt tgttttagtt
    15121 ctgcaatcaa tttgtttctt tgcgctattt cttttccttg aattcttatt cctgattttt
    15181 cgatttcttg taaattgcta tcaattttct tcacatcttc aatttttgat tcatcaattc
    15241 ctaaacgttt tgctttagtc attacttcat caattaattt tctacgggca ttgaccgcga
    15301 aattctttat cgctttctta tccatttttc tttcccttct atatgtaacc tttgaaaaga
    15361 caggcacctt tttatgcctg tcttttcact agaattgaat cgtgatttct gtattttctt
    15421 gaagttgttc cttcagtttt tgattaattt cagctgttaa ctcatcaata tctgctgctg
    15481 acttaatagt tttaacatga acgttaagtg ttctcattgg tactgttttc tgttgaatag
    15541 taggttttac tatcgctatt tcttcagctt ttgctgcaga gtctatttca tctttttcag
    15601 tttcacgaat caagtttgct gcttcaattt gtttacgtgc ttgttcttct tgacgtctgc
    15661 ggttttcttc attatcgaaa cgttgattaa agatcaaata ctgttctgct acgtttaatg
    15721 ataagaaaac aagatcatct atagtattcg cttgttcaat ttttgctttc agttcttgaa
    15781 attcttggtc aactattggt acaaattgat ccttaaaatc ttttccttct aataatttaa
    15841 atgttgcttg tttttcttcc gttaatttat ttaatatttc ctgactttcc aactctaaca
    15901 gttgattata ttgttcaata aattcttgaa tcaactcatt tagtttcatc atatctgaat
    15961 aaggatcagc ctttttcaaa atggttttca tctcatgaat caattgatca atttcttggc
    16021 tagaagtttg acgccgactt ttttcgatta atctaatttt atcttgtgca ttctcccaga
    16081 tttttttctg agtactattt tgcttgtgga aatttttaat tttatcatat gtttcagccc
    16141 agtcgaataa ttcatcgcga tattttttga ttgtataaaa aaagtcttca acatctgacg
    16201 tttgaacaat tacttcaaga tattttttta tttgctcata ttcataagca ctagggtagg
    16261 tataagtgtt ttgtaaatca aaataagagc gtaaattgtc taattgacct tttaaatatt
    16321 tcttgaaatt ttccattgta tgttcattgt tattttcatc aacaatatta cgactttgaa
    16381 acagttctct accgatttcg tcaaccaatt tttttgcttt tggcgaaatc accgcgcttg
    16441 gttcaatttt tatcttttca ctttctcgtc gtttttgaat aatcattaca atactatttg
    16501 gatcatccat ccgtgaaatt tgtacattgt tataaagtaa atcaatttta ccagacatga
    16561 aaagtttagc gatgcaccat tccacatcaa gttctgtgaa tccatacggt tgttttgttt
    16621 caaaacgatc acgaattgtt ttcatcgtaa tcgttaggtg atttctcttt tgatcattga
    16681 taaaattgaa aatttcctga actgcttgct catttgatgg atcttcaata atttcatttt
    16741 tgctttcttt cactaaatat aagatatctt ctttttcttt aggcgcatca atttggttta
    16801 atttatggta tacttcttca gccattttct gaagtgctga ttggaatttt gctttaaagt
    16861 cggagcctgt cagttgaatt tctgcaccgt tatagaaaaa ttttgcagat ttcaacagtt
    16921 catttaattg atcgttgata cttgcactta gttcctgtaa ttcaccagat ttttccgaac
    16981 gaatcgcgtc aaagttcttt actacactcg aataagtcgt tgttaaataa agattcattc
    17041 tagcatacat tgctatatca tctttaaatt ctttatctga tggcaattct acgaataata
    17101 agttacggga actcttaagc gcaagttctg cctcttgacc tgctaaatca gagtatggtg
    17161 aaataaaatt aactgtcatt tcagcatttc cagcttgctt gtatggacgt tgatctaaca
    17221 cttgtgcata agtaaacgta taccggtttt taaatttatt tccacctgac atgcctaatt
    17281 tttcataggc aaaactattt cggtctagaa cgccgtcaaa aattcgttta gatagttcta
    17341 cgatcaaatc actatctgta atattttgat ttttaatctc acgagtaatt tcctgttctt
    17401 catcagtttg gaaaatataa ctatctaagt tcttccgcac aaagttttcc ttaattaaaa
    17461 ggtccaatgc ttcttctact cgttttttag tttccaggcg gtcttcgttg atatttctta
    17521 tcatcaagct cgtaatattt tcaacatttg gtttgatggc ttgatcaaca tatttaatca
    17581 tgaataaaat tcgtaagaca tttacgttaa agttatctat atcagttggt ctttgaatat
    17641 gagaattatt tgtagcttgt gtaatcactc ggctaattgc tgtatcaatc caacgttcta
    17701 acgagtaata aaactgatct aaagaaacca aactggttgt atcattttcc ttatttcgtt
    17761 cagcggcttc tttaaacatt gctagtaatg aacgttcacc agacgataag tttgatcctt
    17821 gatagctgtg attacgaatc tcggttagaa tttgccccaa taaattgaat tgatagggaa
    17881 taaatggata aacttctgcg taattttcag catcttggaa gagctttaat tcaggactat
    17941 caacaaagag taatttattt ttcaattctg tttgaacagt ttcaaattgt aaggctaaag
    18001 aatcattggc aaactctttt ttcttcaaaa tacgttcttt aattacttca tcggcatttg
    18061 ctgaagataa actcacagtt gtggcaaaac gatccttgat cttagaaaaa tcattatcag
    18121 aattgttatc aaagtttgtt acgtgatcaa tcgcttcctg acttgtcact aatacccaag
    18181 ccttaccttc acaaattcga cccaaatctt ccacaacggt ttgtaactgc aaagtcaact
    18241 cacgattacc gccgatatat tgtccgacct catcgactag aaaggcaata tgatggtctg
    18301 cttctttgga atcaagatag cttttaacta gatttgcaaa gtcatcaatg cttattaaat
    18361 aatttcctga tgctttttct aaaaaattat caatagcggc ttcactcata aaatcgattt
    18421 taagtgccgc ttctttgata tcatcttgga tccaatcaaa ctcatggcgc tcttcttgcc
    18481 attttgcccc attaatctct tcaaaagcag ctttgaatgc atcaaacttt tcttctttaa
    18541 ttaaccgacg ttcaaaatct gctagatgtg gaatcgtgct taatccttgc atttcgttaa
    18601 atacctttaa aaaaacattc acgattccgt ttttgtcttt tttaccacct gcatcacttt
    18661 ttgaatcaat attaaacaga ataacatctg tatttatttg tgagcaccgt tccatcgttg
    18721 ccaaagtcag tggatctttg attttcccat cttctttgaa gtaatcaatg gctttcttgt
    18781 catttatttc tcgatttgcg agcaagtaag aaataatttt taagaaatga gatttacctg
    18841 aaccaaagaa cccctgaatc caaacaccca ttttatctgt tggtgtattg atactccgtt
    18901 ggtaggcatc aaaaattgtt ttaaagtgtt tttgtagttc tttcgtaacc acgtattctt
    18961 ctaattcttg ttgaacattt tcatcctttg cttgaccgac cttaataact ccttgaatat
    19021 ttcggtcaat tggttttgca aaaatatctt taattagcat ttaatctcct accttccatc
    19081 ccaactaaat tctaaatgct cgataataat tatcatcttt attggtatta agaattgtca
    19141 aacttaattc atcatattcc cctggataca tcatcacaac tggtacgcga tcaaatacct
    19201 gatgcatttt atttaaaata gagtgcgctc taattaatgg atataattcg ccaattcctg
    19261 tcaggaacac aactgatttt tctggtgtat gggcatccac atattcaaaa aatacatttt
    19321 cttcctcagg taaatccatt ttcaataaac gaccaatgct ttccactaat ttttgtaaac
    19381 cacgtcgttc ttccatctta atcgcttttt cagtgaaacc gcgtttttct agatactcta
    19441 acataatcac atagagatta taaactttca cttcataatt atcaccaaca gatgttccag
    19501 atatatcatt aatataatct cttacaatta actcgtcttt cggatcgtag tcaaagatat
    19561 aatagcctac ttcattacct aaaccacgat tcaataaaaa atcttctttt tgaattactt
    19621 cattcaactt ttccaaatgc tcttgaatcg ataatgacat tataaaattc ctcccagtgc
    19681 tagtacaata tctttacgat tcttgtcaac taaccattga atcaatcgtt tatctaataa
    19741 tatcggagtc attcgtccat attctttttc ccctctgact aatcctgttt caattagatt
    19801 cgtttgataa gcggcaataa gccgtttaat cgtataatct ttccattttg aaatatcttc
    19861 tgaacttctc cgcttctctg taaaaaaaga acggtaatca actggtgtta attgcaaatt
    19921 tcctttacgt agattttcag catatacctc agtcataaat tcattaaaaa gatcatcaat
    19981 cataaggatg gaaatcaaat tcactaactt ttgattatct atatttaact gattgaaaag
    20041 ttgcaatcca gcttcatcca gttgactaac tctccttttc acgttctgat agatttcttt
    20101 ttgacggtta ctgctcactg caaaaaaaat attttcatgt tcatttttct ctttaatttc
    20161 tatatctgta tatccttcta gtcgtaattg aatatataac ttgaactctg aaaaccaaaa
    20221 gcgtttagaa actagaccag cagaatattt tttattcaat agattgcctc ctaatcatct
    20281 ataattcaat aagtaaacaa gtgttttttg ctcatcgtta gcattcattt tttagtatct
    20341 actatacaat acatatttat tata
//
LOCUS       DACCWV010000044        19869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCWV010000044 DACCWV010000000
VERSION     DACCWV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.044"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..899)
                     /locus_tag="IVW84_001340"
     CDS             complement(36..899)
                     /locus_tag="IVW84_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7917545.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1095..3194)
                     /locus_tag="IVW84_001341"
     CDS             complement(1095..3194)
                     /locus_tag="IVW84_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7917546.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3414..4523)
                     /gene="rpoD"
                     /locus_tag="IVW84_001342"
     CDS             complement(3414..4523)
                     /gene="rpoD"
                     /locus_tag="IVW84_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7917547.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4547..6388)
                     /locus_tag="IVW84_001343"
     CDS             complement(4547..6388)
                     /locus_tag="IVW84_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7917548.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6570..8963)
                     /locus_tag="IVW84_001344"
     CDS             complement(6570..8963)
                     /locus_tag="IVW84_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7917549.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9070..9930)
                     /locus_tag="IVW84_001345"
     CDS             complement(9070..9930)
                     /locus_tag="IVW84_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7917550.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9989..10610)
                     /locus_tag="IVW84_001346"
                     /pseudo
     CDS             complement(9989..10610)
                     /locus_tag="IVW84_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            10819..11538
                     /locus_tag="IVW84_001347"
     CDS             10819..11538
                     /locus_tag="IVW84_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7917551.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            11564..14155
                     /gene="mprF"
                     /locus_tag="IVW84_001348"
     CDS             11564..14155
                     /gene="mprF"
                     /locus_tag="IVW84_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7917552.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14343..15530)
                     /gene="tuf"
                     /locus_tag="IVW84_001349"
     CDS             complement(14343..15530)
                     /gene="tuf"
                     /locus_tag="IVW84_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7917553.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15898..16254
                     /locus_tag="IVW84_001350"
     CDS             15898..16254
                     /locus_tag="IVW84_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7917554.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(16495..17499)
                     /locus_tag="IVW84_001351"
     CDS             complement(16495..17499)
                     /locus_tag="IVW84_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7917555.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            17665..18516
                     /locus_tag="IVW84_001352"
     CDS             17665..18516
                     /locus_tag="IVW84_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7917556.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            18533..18778
                     /locus_tag="IVW84_001353"
     CDS             18533..18778
                     /locus_tag="IVW84_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917557.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            18965..19669
                     /locus_tag="IVW84_001354"
     CDS             18965..19669
                     /locus_tag="IVW84_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917558.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5604 a         4074 c         3497 g         6694 t
ORIGIN      
        1 ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt
       61 gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta
      121 aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta
      181 aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct
      241 gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca
      301 ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga
      361 tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg
      421 tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat
      481 ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg
      541 gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca
      601 agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt
      661 cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta
      721 gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt
      781 ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag
      841 tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt
      901 tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc ataacataat
      961 gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca
     1021 gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt
     1081 tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg
     1141 gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt
     1201 gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg
     1261 taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca
     1321 cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat
     1381 ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt
     1441 gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct
     1501 tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct
     1561 tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga
     1621 gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg
     1681 cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct
     1741 gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca
     1801 aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca
     1861 tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata
     1921 tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca
     1981 ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat catatctgca
     2041 cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc
     2101 atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca
     2161 taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact
     2221 ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga
     2281 tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta
     2341 ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat
     2401 tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg
     2461 ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat
     2521 tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt
     2581 actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt
     2641 tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc
     2701 gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt
     2761 tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca
     2821 agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc
     2881 tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt
     2941 tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat
     3001 tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc
     3061 atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt
     3121 atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt
     3181 ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc
     3241 tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa
     3301 tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt
     3361 ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct
     3421 aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc
     3481 tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt
     3541 ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc
     3601 aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct
     3661 ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg
     3721 attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct
     3781 gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc
     3841 agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata
     3901 cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta
     3961 gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt
     4021 cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta
     4081 gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg
     4141 ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg
     4201 ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa
     4261 agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat
     4321 ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg
     4381 tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat
     4441 acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt
     4501 tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt
     4561 ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc
     4621 cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag
     4681 atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg
     4741 gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa
     4801 actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt
     4861 ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa
     4921 cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc
     4981 tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt
     5041 gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc
     5101 cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa
     5161 ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta
     5221 aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag
     5281 ccactaattc ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct
     5341 tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc
     5401 ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg
     5461 tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc
     5521 cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa
     5581 tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt
     5641 tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt
     5701 tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg
     5761 gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca
     5821 atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa
     5881 aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc
     5941 cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat
     6001 gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat
     6061 tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg
     6121 ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca
     6181 cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa
     6241 aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt
     6301 tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga
     6361 taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg
     6421 ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag
     6481 tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca
     6541 agtataacac atttctatct cattaagcat caatttgcta ctcttttcag taactgattt
     6601 tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag
     6661 aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt
     6721 gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag
     6781 ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa
     6841 gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata
     6901 ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg
     6961 taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc
     7021 gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca
     7081 catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc
     7141 gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca
     7201 gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca
     7261 atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca
     7321 accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact
     7381 cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga
     7441 gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca
     7501 gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct
     7561 agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca
     7621 acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg
     7681 cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa
     7741 cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg
     7801 cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa
     7861 tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata
     7921 atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt
     7981 ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt
     8041 tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc
     8101 gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct
     8161 gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat
     8221 agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa
     8281 tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca
     8341 taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt
     8401 ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga
     8461 gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct
     8521 gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta
     8581 ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt
     8641 cttgcatgta tttcttgata aattccaata acgatattga cgacaataat tgctaaatac
     8701 gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa
     8761 ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc
     8821 tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca
     8881 ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta
     8941 gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg
     9001 attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc
     9061 aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc
     9121 ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt
     9181 aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct
     9241 atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc
     9301 accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc
     9361 tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc
     9421 caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc
     9481 aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat
     9541 gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt
     9601 tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa
     9661 actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa
     9721 aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc
     9781 atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga
     9841 accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc
     9901 ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt catttaattt
     9961 tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta
    10021 ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat
    10081 agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat
    10141 ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt
    10201 ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat gcttggacag
    10261 ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga
    10321 caccttgatc aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt
    10381 ccgagatctg tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg
    10441 tagttttcca tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact
    10501 cgttttaccg aacgataagt tcttcctcct aaaaacaaca ccgcaccaat aaccgtcaga
    10561 atgatcatgt aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct
    10621 agttattttg acatcttttt ttatcttact atttctttat cttcttggct atttcttcct
    10681 tgatttctgt gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga
    10741 aaatgatcgt tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt
    10801 tacacagtag gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc
    10861 gaaatttact atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac
    10921 ggcagcggcc gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat
    10981 ctagtagata gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag
    11041 ttaatggccg aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt
    11101 cttggattta gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa
    11161 gttcatcgat tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc
    11221 agagtcatca gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt
    11281 cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat
    11341 gatttaaaaa aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag
    11401 ctaaaacatt ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa
    11461 gaacaaggac atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa
    11521 tttctttctg aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat
    11581 acaatggctg aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt
    11641 cattattgtc ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga
    11701 aacgtttgcc actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc
    11761 tgtgttacct atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa
    11821 tcctcgttac ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt
    11881 cggcgggttt gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag
    11941 aaacgtgatc caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta
    12001 cagtctgctt tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata
    12061 ttggatatgg ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt
    12121 caaaaagaaa ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt
    12181 ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga
    12241 aatccggatt gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat
    12301 cgtgtctatg attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc
    12361 cgctattggt gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta
    12421 ctacatcatt ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt
    12481 tgatcagcgt tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt
    12541 ggttgtctta ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc
    12601 ttttacagag tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca
    12661 atttcctagt atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg
    12721 tgtgaaacga gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt
    12781 attaagtgat tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct
    12841 tgcttctaaa aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat
    12901 cgatggaatc attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct
    12961 gccagatttc cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa
    13021 gatttggttt tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt
    13081 tcatttcctt caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct
    13141 gaagatcctt acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa
    13201 gcggatgttt gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt
    13261 caataataaa tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc
    13321 aatcgaagct tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac
    13381 aagcgaagag attgttatga ttttgcatga attcggctac gatttcatta agatggggga
    13441 agaagcttat gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg
    13501 tgctgtcttg aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt
    13561 ttctactgag caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa
    13621 ggaaaaaggg ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc
    13681 agttgtcaaa aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac
    13741 gaacaataaa gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg
    13801 cagtatggat ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg
    13861 gttcaattta gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca
    13921 ggaaaggatt gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt
    13981 aagagaatat aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag
    14041 agatagttgg attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga
    14101 gcagacacaa ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact
    14161 gcataccgat agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt
    14221 catgcttcta caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc
    14281 ctgtttggac cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt
    14341 tcttatgctt cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt
    14401 gagaatgtcg ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg
    14461 ttgtcaccag gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca
    14521 gttgtgcgga agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct
    14581 tcttcttttg tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct
    14641 ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg
    14701 cctacgttat ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt
    14761 acaactgctt tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga
    14821 acagcaccac gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg
    14881 tcttccactg gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca
    14941 tctactgtat ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct
    15001 tttaatgcgg aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat
    15061 aactcgcgaa cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact
    15121 ttgttcaaga aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca
    15181 cgtgtttgag gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt
    15241 gctgcccctg taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg
    15301 tagtggcgtt tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca
    15361 cgttcttctg gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg
    15421 cctaatactg tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc
    15481 gtaccaatgt taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa
    15541 cctcctaaat aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt
    15601 ttttgactaa atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt
    15661 attatacgtc aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa
    15721 aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa
    15781 ctttcttttg aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac
    15841 aactttcaat agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg
    15901 aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct
    15961 tatcattatt tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg
    16021 tcagctaccc aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct
    16081 tataataaag atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta
    16141 aagatgaatg cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa
    16201 gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg
    16261 gtcttaaaat tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
    16321 agaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
    16381 ctttactttt gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga
    16441 cacaattcat ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa
    16501 acgattcaat acttcatcca catttttgat catcacggat atcgtaccat ccggattatt
    16561 cacaaattca atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc
    16621 gtttgatagc ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt
    16681 tacaggaggt gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt
    16741 tgccgtgatg ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt
    16801 ttcttctatc gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt
    16861 ctcaaatttt tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa
    16921 agaaggtgca ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga
    16981 aaactcatat tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc
    17041 ttcatctgct ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc
    17101 gatccctgct tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa
    17161 taaagataag tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg
    17221 tgcttcttca ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc
    17281 cacaaaattt tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg
    17341 tccagttttt gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat
    17401 atattctttt gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc
    17461 aataatatgc agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt
    17521 agtttacgga aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat
    17581 ttcaagagta gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga
    17641 acgaataaaa aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat
    17701 ggttacgaaa tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct
    17761 gtttcctctg ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga
    17821 tacaagaatg aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag
    17881 atattcttga caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct
    17941 tttgaagaat cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg
    18001 ccaaaccctg ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca
    18061 tttgaagaac tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc
    18121 caccatctag atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc
    18181 cttgcaccag gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc
    18241 ctcgaagcat atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa
    18301 atcgctgaag cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac
    18361 gaattccttc ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta
    18421 tttgattttg aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc
    18481 ggcaaagcaa aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc
    18541 aatcgaacgc ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt
    18601 ctttttgatc gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca
    18661 aatcatcgaa gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga
    18721 acatgaagtc atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa
    18781 agaatggaac ggattatgta tatatacata atccgttcca ttttttattt ttctttcaaa
    18841 aaacgggaaa atacgcaaat ttttctgttg taatttgcgt atacgcaagt ttttttgcat
    18901 tactcctttt catttggctg tttttttctt ataatcgaaa gggtatgagc gaaggagaga
    18961 acatatgaag aaaaaacaga aacaaaaatt attaaatcaa ttccgtcctt cattggatgg
    19021 tgtaagagga caattattcc gttctctaga agaagagagc ttgcgacgtt atggtttgcc
    19081 gttacaagtc atgcttgatc ccaaaaaccg tcaaatctta acgatcggat tagctgggaa
    19141 aacaaatgaa gatacacgga tcaacgtgcc aatcgatgac aacttcaata ctgtattaaa
    19201 gagaattcgt tcaggagaaa acgggatttt cgaacgattc cgtgacaatc ttcttattga
    19261 aatcgtgtct tactggaatg atcaacgatt gaaaaacgat gaaccaactg cgcaagctgt
    19321 tgcgacacca gttacagaag aaacagtcag tgtgccagaa gaaaaaacgg aaaaaccaaa
    19381 agcggacaca actgaaaata cacgctcaac tgatacgaat gccgctttaa gttttgctga
    19441 tttttcaaaa gccgttgctg agtatcctaa attttacaca gaacaaaatg aacatgaagt
    19501 agttattaat gaaaaaaatg ctgatgaacc tcgtaaatta gcaactattt ctatgactac
    19561 tgaaaatgag ttcgttatcg aaaaagcact cgaacggaaa tacaaagtca aattgacttt
    19621 gattcctttg atcgaacaat ttgcagcgac agcgattact gatcgataaa aattctattt
    19681 gtcataaaag gctgggattt aaaatcccag cctttttagt catataataa tttctacagc
    19741 aagtaaaaag aaattccttc taacatcagg ctcaaatatc agcttgacct gtccaacaaa
    19801 tcacctatga tcctacaatg ttaggcgact tctcttctac ctgaagatat cttggagttg
    19861 cctgatttc
//
LOCUS       DACCWV010000045        19509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCWV010000045 DACCWV010000000
VERSION     DACCWV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.045"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(462..1091)
                     /gene="udk"
                     /locus_tag="IVW84_001355"
     CDS             complement(462..1091)
                     /gene="udk"
                     /locus_tag="IVW84_001355"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7917559.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1175..1870)
                     /locus_tag="IVW84_001356"
     CDS             complement(1175..1870)
                     /locus_tag="IVW84_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7917560.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1883..2599)
                     /locus_tag="IVW84_001357"
     CDS             complement(1883..2599)
                     /locus_tag="IVW84_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7917561.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2929..3534
                     /locus_tag="IVW84_001358"
     CDS             2929..3534
                     /locus_tag="IVW84_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7917562.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IVW84_001359"
     CDS             3859..4182
                     /locus_tag="IVW84_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7917563.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IVW84_001360"
     CDS             4364..5734
                     /locus_tag="IVW84_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7917564.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IVW84_001361"
     CDS             complement(5864..7165)
                     /locus_tag="IVW84_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7917565.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7395..9386)
                     /locus_tag="IVW84_001362"
     CDS             complement(7395..9386)
                     /locus_tag="IVW84_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7917566.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9530..9796)
                     /locus_tag="IVW84_001363"
     CDS             complement(9530..9796)
                     /locus_tag="IVW84_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7917567.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10306..12432)
                     /gene="pnp"
                     /locus_tag="IVW84_001364"
     CDS             complement(10306..12432)
                     /gene="pnp"
                     /locus_tag="IVW84_001364"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7917568.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12620..12889)
                     /gene="rpsO"
                     /locus_tag="IVW84_001365"
     CDS             complement(12620..12889)
                     /gene="rpsO"
                     /locus_tag="IVW84_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7917569.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13140..13703
                     /locus_tag="IVW84_001366"
     CDS             13140..13703
                     /locus_tag="IVW84_001366"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7917570.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13956..14477)
                     /locus_tag="IVW84_001367"
     CDS             complement(13956..14477)
                     /locus_tag="IVW84_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7917571.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14480..15322)
                     /locus_tag="IVW84_001368"
     CDS             complement(14480..15322)
                     /locus_tag="IVW84_001368"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7917572.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15315..16040)
                     /locus_tag="IVW84_001369"
     CDS             complement(15315..16040)
                     /locus_tag="IVW84_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7917573.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16216..16512
                     /locus_tag="IVW84_001370"
     CDS             16216..16512
                     /locus_tag="IVW84_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917574.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17080..18672
                     /locus_tag="IVW84_001371"
     CDS             17080..18672
                     /locus_tag="IVW84_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7917575.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18685..19425
                     /locus_tag="IVW84_001372"
     CDS             18685..19425
                     /locus_tag="IVW84_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7917576.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5581 a         4002 c         3702 g         6224 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa aacagttggc tggtgactgt tcaaaaagca gaatccttgc
      421 ttttcaaacg gtcgcagtcc gttcaactgt ttcgctgcgc actatttatg gtttaagaaa
      481 ctagctactt ttgttgtaat caaatcaata gcaacgtggt tttctccgcc ttctggaaca
      541 atgatatccg catagcgctt cgttggttcg ataaattgat gatacatcgg tttaacaact
      601 gtcaaatatt gttcgatcac ggagtccaat gtgcgtcccc gttcttccat atcccgtttg
      661 atacgacgaa tgatacgaat gtcatcatct gtatctacgt atactttgat atccatcaag
      721 tcacgcagac gttcgtcttc taaaatcagg attccttcta gaatgatcac ttcttttggt
      781 tcctgaatga tcgttgcctg acttcttgta tgcgctacat agtcatacac gggtttttca
      841 atggctttat agttcagtaa gtcgcctaca tgctgaatca gcagatcatt atcaaaggca
      901 aatgggtgat cgtaatttgt gttcaatcgt tcttcaaaac tcaaatggct ctgatccttg
      961 taataagaat cttgttcaag catcatgatg gaatggtccg ggaagttgtt gaaaatcgca
     1021 cggctgacac ttgtctttcc gcttccggat ccacccgtca caccaatgat aattggcttg
     1081 ctttttgtca ttgactaaaa cctctttcgt atccgttttt tcattctaat ctattatagc
     1141 gaaaaaacac gaaaatgcta tatttttctg aaaattaatc cttttcaatt tccaataatg
     1201 aataaagttc ctctaatgaa tcgattcgat aagtaggctg gatttcttcg atttctggta
     1261 atttatttct attgaaccaa atcgtatcga tccctgccat atttcctcct ttgatatcag
     1321 aagtcaaaga atcaccaatg atcacagttt gctttttatc aaaattagga atgtgggaaa
     1381 agacatgatc aaaaaattct ttcatcggct tttgcgctcc gacagcttct gagacaaaga
     1441 tatccttgaa gtaatcaagc attcctgcat ctgttaatcg ccgatgttgc gtgtgcgaca
     1501 caccgttcgt cacaatataa agatcagcat gcggatggac ctttttcagg atttctagac
     1561 ttcctggaac aagatcatga ccttggttca agtagtggcg gtagcgtttt tccatttgat
     1621 cactatctac tgttttccca aattgctgga aaagtaaccc aaaacgactg cctattattt
     1681 cttctcgtga tagtctgcct ttttcaaact cctgccataa atgatgattc aaagccttat
     1741 agctggtttc gatttctggt gtcaatgtga ttccttgttc tgcaaacaaa gcacttaaag
     1801 cattggcttc agttaattga aaatctaaca atgtatcatc tacatcgaac aataaagttt
     1861 tatatttcat ctttttgacc tcctatttaa aataaatact taactttttc atctctgcta
     1921 atgtcaacga tcgatactcg cctactccta gctgttcatc taattccaat ggtcccatgc
     1981 ttagccgttt caatgcggtc actttctttc cttgggctaa gaacatcttt ttgacttgat
     2041 ggaattttcc ttcttggatc gtcaataaaa ccgtactttg gtgcttgtct gctttgagta
     2101 tttttaaatc agccggctta cattgatacc catcagcgaa tacgattcca ttttcaaatg
     2161 ctgttacgtc tgccggtgtc accctttcgt tcacaactac ttgataagtc tttgaaacct
     2221 ttttttctgg aagtagcatt tcatatccca attgtccatt cgaagtgatc agcaatagtc
     2281 ctgttgtatc tctatctagt cttcccactg gatataatgg ttggaaacga tcggcacttg
     2341 caatcagatc gattactgtc gtcttttctg gatctgatac agctgtaaca gctttttcag
     2401 gtttattgag aagataatag gactcatttg tttctaagtg ttcacctgct acttcgattt
     2461 gatgcagtaa actatctaca tttcgatttt gtcgaaattc gactgtgcca tctatcatca
     2521 cttgcttcaa agcaaacagc cgcttcattt ccttgcgtgt cgtttctaat ttttcttcta
     2581 ttaatttatc taaacgcatg ctgtttcctt tcttattact actaattaat tataccttga
     2641 atattcttgc ttgcaaatac atcatcatag ttattccttg acttaattaa cagttcacgt
     2701 tacggttaaa tagtaaagtg aattggattt ctcatacaca gcgtaatgac ctggtggaga
     2761 aaagttgaat cctgactttc taaggggaga tgcgcgcatc tctccttttg cgctttttta
     2821 agcgccctgc cttcgatgat gtgcacgaga ttagccttta gaattcactt aaataaaatc
     2881 tgtccgttac caaagcagac agtcacattt taaggaggaa ttttggttat gtcagtatca
     2941 ttagaagtaa gtaaaagaga agtacgtccc cgttcattaa gaaacaaact tcgtcacgaa
     3001 gggaaagttc cagctgttgt ttatgggtat aaagtagaaa gtacccctat ctcattcgat
     3061 ggtttgacac taagcaaatt gttgcgtgaa catggagcga acacagtcat cacgatggac
     3121 atcgatggac aaaaagtcaa tacattggtt cacaagatcc aaacgaacac ttttaccaat
     3181 catttcgaac atgcagaatt tctatctgta aacatgtcgg aagaaacaga agtcgaaaca
     3241 gaagtcgttc ttgttggcga atcagtgggt gctaaatctg gtggagtgtt agctcaaaac
     3301 ttgtatacag tagtcgtatc tgctacacca gacaaattgc ctgaacgaat tgaagttgac
     3361 gtgacaaact tggaattagg tgattctatt acagtagccg atcttccaaa aaacgacgat
     3421 tacaaattcg tgaccgacag tgaagagcaa atcgctgctg tagtcgctca aagtgaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct atttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gctttccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa tgatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaacggtgtt caagtagctg acattcgaca ttagttcagg
     7381 tcaaagcgct actttcacaa ccgctctttc aaccatgttt tccgagtatt tccaatgata
     7441 agcggaacag tttcatgttt tacttcgctg tactctagcc caaaccatgt ttctggcgag
     7501 gtagcgattt ttttgattgc cagttttgcc tgcatgattt gacgttgcca ttttcctaga
     7561 gttgtcacat tggaaactac ttcatcaatc aggacaaatt ggaaatcgcc aacttctcgc
     7621 cctggtgtca gagagtatct ttgtggctgt tttggcaaac ggccttgttt catcaaatca
     7681 tggatgactt ggcgaacata tacgttgatt tcctgttgga ccctgaaacc taagaacaga
     7741 ttcacttgga caataaaatc agtgtccatc atatctactt gatattcttt cgtatatggc
     7801 tcatccgtca cttccacatg aacaaaccaa tagactcgtg ctcttttagg ccgtttatct
     7861 aaaatcgaat agatgaactg tcggccaact ttatcttcag ccatatcagg aaccaagaag
     7921 accacatttg tctgatagat tggtaaagaa gtatcatcac gtaattctct aagctgcggg
     7981 atataatctc tcaaagacac atcctcggtt gacgattcgc gaataatgtt cccttgttcc
     8041 caaataaaca tcacgactaa gatgatcaaa ccaatcagaa cggctacaaa accgcctcgg
     8101 aagaatttga caacacttga gataaagaaa acgacttcta tcgcaccgaa gaaaaaggta
     8161 actaaatatg caagccaacg agaatgccca ttttggataa gataaaagta aagcagtgct
     8221 gttgtcatca acatcgtcac agtgatcgat agaccgtaag cagcttccat atgatgagac
     8281 gttcggaatg tgacaaccac tagagaacag gcgatccata aaatcagatt gactgctggg
     8341 atatacattt gcccgatact gttcccagga tacatgatct ttaaccgagg cagcagtttc
     8401 aacttgattg cttctgaaac tagcgtaaac gaacctgtaa tcaatgcttg cgaagcaatg
     8461 actgcagcga ctgttgcaaa agcaacgccg atcacgatcc aactttgagg catcatttgg
     8521 aagaatggat tcaggttctt gatttgctgt gcttcagggt tttgataaac attcaacaac
     8581 cacgcagcct gcccaaaata attgataacc aaacaaattt tgacgtaagg ccagctcaga
     8641 cgaatatttc gtttgccgac atgtccaaga tcggaataaa gcgcctctgc acctgtcgta
     8701 gccaggaaaa tatttccaag aatgaacaag cctaatttgt tatctgcact aaaaagcagc
     8761 tggatagcat aataaggatt cagtgcacgg ataactgtcc agtcttgact gaagttcatt
     8821 aatcccatta cgcctaaaaa agtaaaccaa gcgaacatga ttggtccgaa tgcttttccg
     8881 acaatatctg taccaaagcg ttggaccatg aataaaacaa aaataataac taaagtgata
     8941 atgacgatca cgttctggtt attaccgaac cggtcaaaaa actgcggaat tcctcgcaag
     9001 ccttcaatcg cagtcgttac cgtcacagca ggggtcaata ccccatctgc tagtaaggca
     9061 gctcctccga tcattgcagg aatgatcaaa tatttcccac cttttcggac aagtgtgtag
     9121 agtgagaaaa ttccaccttc accatggtta tctgcattca atgcaatcac gacatatttg
     9181 attgttgtca gtagagtcaa tgtccaaaaa atcaatgaga ctgatccgat aacaaaatca
     9241 ggattgattc ctgacaatcc gccattatct tcaacaatcg atttcataac gtaaagcgga
     9301 cttgtcccga tgtctccgta gacgactccc atcgctacaa gcaggccagc cattgatatc
     9361 tttttggtat tgtgcgttgt tgccacgttt ttccctcatt tcttgttata tggatatcct
     9421 ttaggtaagc ttgaatgata gatgatattc ttatccatga cacccgcttt cctaaattgc
     9481 ttctaattat ccaagtaaca cactgaagtg tcaacttatt tgaataaaat tattctttct
     9541 ttggacgttc tctgatacgt cttgccacaa atgaataaag agcaaggcaa aaaattgcac
     9601 ccaataatgc tggtatcaaa taaaaaccgc cgagttccgg tcccagtggt cctagtaaaa
     9661 attcgccgag ccaactacct aaaaaaccga taatgatatt tccagatacg cctcccggga
     9721 cttcctttcc taaaattgcg ccagctatca aacctaatat accgccgata aataacgacc
     9781 ataaaaaatt cagcatctcc tttccctcct ttatccatat ttctcacttt tattttacgc
     9841 tttattcaga aggactccaa aaatatgcgc caagaaactc cagaaaaaaa gaagttgtaa
     9901 caaaagtgtt cagcttattt ccgtagaagc tgcttctgga acaccgtgaa tcagaggttg
     9961 tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
    10021 ttgccgtttt ttgagctaat atcgagggac agctgattga acatcgttta ttggagaggt
    10081 tgtaaaaaag ctgttctgca tcaagtaata agaacagcca agtaaaaaca gctcctaatg
    10141 tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
    10201 aaaaaaggac tgtgacacgg cttttgtcac agtccccttt tttcgaataa ttattgtccc
    10261 cctgtcacct tcacgatcac aagtacatta ttcctatgaa ttaaattatt tttcttcatt
    10321 tttcgttgaa ccatcttcgt tcaacattgc ttttcttgac agatttacgc gtccttgttt
    10381 atcgatttca gttactttca caaggatctc atcacctagt ttaacaacat cttcgacttt
    10441 attgacacga tcgtttgcta attgggaaat atggacaagt ccatcttttc ctttgatcaa
    10501 gttgacgaat gcaccgaatt tttcgatacg gacaactttc cctagataaa cttcaccaac
    10561 tttgacttct ttggtcaaat cttcgatgat cttgatagct tttttgatca tttctgcatc
    10621 agctgaagca atacttactt ttccatcttg atcgatatcg attttcacac cagtttcatc
    10681 gatgattccg ttgatcgtat cgccgccttt accgataaca tctttgattt tagcaggttc
    10741 gatctggatc atttcgattt ttggtgcata ttggcttaat tcttcacgtg gagcagcaat
    10801 cgttgaagtc aattcttcta agatttccat acgtgcttgt tttgcttgag ttaatgcttc
    10861 agttaggatt tgttctgtga ttccttgaat cttgatatcc atctgtaaag cagtgatacc
    10921 atcttttgta ccggcaactt taaagtccat atcgccaaga tgatcttcca gcccttggat
    10981 atcagttagg atcgtatagt tttcaccatc agaaacaagt cccatcgcaa taccggcgac
    11041 tggcgctttg attggtacac ctgcatccat caatgctaat gtgccagcac agatacttgc
    11101 ttgggaagaa gaaccatttg attccaatac ttctgctact aaacggatcg tataaggaaa
    11161 ttcttcttct gaagggatta cttgtgctaa cgcacgttca cctaatgcgc catgtccgat
    11221 ttcccgacgc cctggagagc ctgcacgtcc tgtagaacca acagaaaatt gagggaaatt
    11281 gtagtggtgg atgaatcgtt tgctgacttc cacccctaaa ccatcaatga tttggtgctc
    11341 tcctagaggt gcgagtgtac aagctgataa agcttgtgtt tgtcctcgtg tgaacaaacc
    11401 tgatccatgt acacgtggca ataagctgac ctctgatgac aatggacgga tttcatccaa
    11461 tttacgcccg tcaggacgga tcttatcgat ggtgatcaat tcacgtacga catctttttc
    11521 aagatcttct gtgatttgtt taacttcttt caacaagtga tcttcatctt catgacctgc
    11581 aaatttctca gcataagctt cgcggatatc gatcttcacc tgctcgatat tatcttcgcg
    11641 agcaagtttt tcttcagtca ttactgctgc tttcattgta gcgtaagaag catcaaagat
    11701 ttcttttttc aatacttcat caacttgcag taaagtcact tccatttttt ctttccctac
    11761 tgcttgaacg atttcttctt ggaaagcaac taactctttg atagcgtcaa aaccaaacag
    11821 caatgcacca agcatgtctt cttctgaaac ttctttcgcg ccgctttcaa ccatattgat
    11881 ggcttgtttc gttccggcaa cagtcaattc gatatctgtt tgttctgctt gttctactgt
    11941 tggattcaag acgtactcgc catttacacg accaacttct acgcctgcga ttgggccgtc
    12001 aaatgggata tctgagattg atagtgctaa agaagaacct agcattgctg ccatagctgg
    12061 cgagcagtct gtttctacac tcatcacgat gttcgttact tggacttcat tacggaagcc
    12121 ttctgcaaac atcgggcgaa ttggacggtc gatcaaacgg gcagtcaatg ttgcttccgt
    12181 acttggacgg ccttctcgtt tgatgaaacc tccagggatt ttccctgctg catacatttt
    12241 ttcttcataa ttgatcgtta atgggaaaaa gtctgtatct tttgcttctt tagaggccac
    12301 agctgcactt aagactactg tatctccata acggactaaa acggctccgt ttgcttgttt
    12361 tgctagctga ccgacttcga tttgtaaggg acggccgccc catgtcgttt tgaatacttg
    12421 tttttctgac ataacttctc cttttccttc tcgtgaaagc aagacgctac taaacaaatg
    12481 aaaactgccg aaagccgtct tttgagcagc tttcatttgg ttagtaggag tccatctagc
    12541 ttgatcacgg aattttactt aattgatata cgagtaaaaa aagtgagatt catgtgaatc
    12601 ccactctttt tgactaaaat taacgacgca agcctaaacg tttgatcaat tcgcggtaac
    12661 gttgaacgtc agttttacgt aagtaagcta acaagttacg gcggtgacca acttttttca
    12721 ttaggccgcg gtatgaatga tggtcttttt tgtgcacgcg tgcatgttca ttcaagtggt
    12781 tgatttcttc agttaataca gcgatttgta cttctggaga accagtatcc ccttcatggc
    12841 gagcgtattc gttgatgatt tcattcttgc gttcttttga aattgccatt tctttcacct
    12901 ctttcgatta ttcccctgcg actgagtaaa acgttggtga ttcgcttaac caagtggcag
    12961 gtcatgtgcc tagagcacac agctatactt tacctgaaac cgggttgaaa atcaagtagt
    13021 taatcattaa gaaaaggtag ttttcaaaca atcttactac tacttttcca tttttgtggt
    13081 aaactataat gcgaaaatag acttgaaaca cagtctttta agaaaggggt acatattcca
    13141 tgattacaat ggatgatatc attcgtgaag gaaatccgac cttacgcgaa gttgcaaaag
    13201 aagtttctct tccattaagc gaagaagata tctctttagg aaaagagatg cttgagttct
    13261 taaaaaacag ccaagatcca atcaaagctg aagaactaca cttacgaggc ggtgttggct
    13321 tagctgctcc tcaattagat atttctaaaa gaatcattgc tgttcacgta ccaagtcctg
    13381 acccagaggc agatggacct tctctaagta ctgttatgta taatcctaaa attttgagcc
    13441 attctgttca agatgcttgt ttaggggaag gtgaaggctg tttatctgtt gatcgagaag
    13501 taccaggata tgttgttcgc catgccaaaa tcactgtgtc ttattatgat atgaatggtg
    13561 aaaaacacaa gattcgtttg aagaattatg aatcgatcgt cgttcaacat gaaatcgacc
    13621 atatcaacgg tgtgatgttc tatgaccaca tcaacgatca aaatccattt gcactaaaag
    13681 aaggcgtatt ggtgatcgag tagagaattg gcaggttcct tcgatactat gctacaagaa
    13741 agctttataa aaaaggggag tgctcaaaag ctgcacttcc cttttttatg tttctccact
    13801 aatcgaaatt tagatagaca tctagaaatc gcaattccaa aaaattctgt gtattggata
    13861 cgcatctttt cttatttttt cttatttctt ccatatgggt gtttatctca gagatgatag
    13921 aatagtttct aggataaaaa gtagcagcta cgatgttatc cttcgtagag gattcctgct
    13981 tcagctagtt tttgtttgat gtttttcagc atatgctttt ctgggtagcg gatcgtatgc
    14041 agatggatgc cgcctgttaa ttcggataac aaagtagcat tacttttttg ataacgtctc
    14101 ataaaggaat cgatatcttg tggcgttttg atgtgcaaag ttcctgctaa ctcaccgtaa
    14161 aggggatgtt ccacaattac atcaatgatt tcaccaccat tttcaacgat tagtctcagt
    14221 tcttcttctg tttgatcttt tccatgctgg acagcgattt tactgatgtg cccagtattg
    14281 gtttcttctg tttcgaggat ataacctctt gctgttgcca cgatactttt acctgcagct
    14341 cgtagtaaag caatatcgcc tacaatgatc tgacggctga ctccaaaagt tttcgcaaac
    14401 ttgctagcac tcactggttt ttctgtattt gccaaggttt gcatgatttg ttttcgccgt
    14461 gtctctcctt cgatcacact tattcctcct tcattttgtt ttggtattcg cgcgagtgtt
    14521 tcatcaaatc aacatacgtg tctgttattg tttccttata tcccttattt ttcagcagac
    14581 taacaacttt atccaatact tgctgctctc tttttgcatc ataaactgct tggttttctt
    14641 cttttttcac ttgtcctatt ttagttaccg tatccattct tttttctaat aaagcaacca
    14701 actctcgatc gatcatatcg atcttatgac gtaatgtttc aagagactct tctgttagtt
    14761 cttcctctac tgtcttgatg atacgagcag gatttcctgc aatcactaca ttgtcgggaa
    14821 aagatttggt cacaaccgaa cctgcgccga ctactacgtt gtcccctaga gtgacacccg
    14881 gcgtgacgat aactcctccg cctaaccaaa cattattacc gattttaatg ggttttgcgt
    14941 actccagacc gctgttacgt ttcactggat gtagtggatg cgtcgccgtg taaagctgga
    15001 cattcggagc gaacatgcag ttatcaccga tttcaatcgt gctgacatct aaaaatgtac
    15061 aattgaaatt tgcataaaaa ttctccccaa caaatatatt atatccgtaa tcaaaattga
    15121 taactggctc catatagatt tttttccctg tacggccaaa agtctctttc aacaattggg
    15181 atcgcagttc cgctgattct gcttggttga tgatttggct ttgacttctt gcaaacatac
    15241 ggtcggcaac cagctcagga tcattggcaa aataaagctc gcctgcaatc attttttctt
    15301 tttcggtttt gaattcagtc ataatctgct cctaattctt gtattttttt gaaaacaacc
    15361 gctccgtctt cttgaataaa cgtataaggc tccgctaaat aaacaggcgt ttgattgatt
    15421 actaagattt tggctgacgg ctggcgaaac tgtaccagat cacaaaacgg atgaacttga
    15481 aaactcgttc caacaatgac gatcagctct gcttggctga cggcataagc cgcctgctcc
    15541 atgactccat cttgaaaccc ttcaccatat aacacgatgt ctgggcgtat ttgtcctccg
    15601 catgcttgat gtttgtcgct gtgaagatat tctttccagt ctaccgtttg ttgacatttt
    15661 cgacaatgac attgatacag actcccatga aaattgacga gttgcgggct tttagccttt
    15721 gcgtgcaaac cgtcgatgtt ttgggaaaca acccaaacat ttttttcttt cgctagatgt
    15781 gctatttcca aatgaatgat attgggtttt gcttcaggat gataaatctt tttgacaaat
    15841 tgatagaatt tatccggttc attcaccatt gcttgatgac tcagcaagta ttccggctgc
    15901 tccatcccgt ggtagattcc tgacaaagat cgatagtctg gcactccaga aggtgtggat
    15961 acccctgcac cggtcaaaaa tgtaatttgc tgataagcta atatctcttt tgctgcttcg
    16021 tctactgtta attgtttcat gttttgtcac ctcactttcc attatagccc atactcgaac
    16081 aaatttattg ttgaaaagtc agaaaaatgg gaataaaaaa gtgttatggg catttgagca
    16141 atttatcaca atgttcactg acaaaagaat gaaaacgtgt tacaatactg ctgtataaaa
    16201 gtgaggtgaa tcgatatgtt accattcttt atttattggg gcgttattct ggcttgtatc
    16261 gcctggttag cgttaagcgt ttatttctct gttttttatc ttgtacgcaa agaaaatgga
    16321 aatctttggg cttttgcttt attcaacgtg attgccgcaa ttgttttagc cattacatta
    16381 gccgtatatc gaacatgggg ctggggaatc acccaatact caagtttgat ttatctaata
    16441 ttagccattt atggcgtcgt tgttcttttg caagcgatcc taggacgtga gccaaagaaa
    16501 gcggcagctt aaatgatcga gatggaactg gacacaggaa attttctgtt ctagttcttt
    16561 ttttattgta ttacatacat ttttgtgtat aattatacta tcgtttgcaa tggatagatg
    16621 attcacattt tgattgtttt ttcggtaaaa gtaaagattt ctatatttat cagtattttt
    16681 tcaaaaacca gcagttatag attgacaaag cctctcgagt atcgtaaact ttttttgtat
    16741 aaataactga atacgagatg cctcaatcaa tgaggtagag gtcgcgagaa tcattaaccc
    16801 tgtggagtga agcaaacatg gagaagcagg aatagggagg atcgccgaaa tgtgcacttt
    16861 tttgcttgga gtgtatgttg ggacgcaagt taagagcttg cggactgtct tagcagtgat
    16921 gctaagttgc gctatgtttt gtgagaggtt tatcacctta tggaatttta gcggcctttt
    16981 tatatctata aatcggtcgc tttttatttt ttcagataaa gctttaccgc atttcatcta
    17041 gaccgctcgt gtatcatata atcagaaagg gagaaaaaaa tgaaaaaaaa tatggtttca
    17101 gttgccgtac tatttgcttt tgtcttctca ctattcagtg tgggatctct ggcaaatgca
    17161 gatgaaaaaa caccgagcga tgagtttcgc gtaggtatgg aggctgggta tgcacccttc
    17221 aactggtctc aacaaacaga tgagaataat gccttgccaa ttcaaggcca aaatagttac
    17281 gccggcggtt atgatgttca aatcgctaaa aaagtagcag atggtttagg gaaaaaatta
    17341 gtcatcgtcc aaacgaaatg ggatggtctg gctcctgccc ttcaatcagg caagatcgac
    17401 gcgatcatcg ccggaatgag ccctactgct gaacgacgaa aagaaattga ttttaccgat
    17461 ccttattacg aatcacaatt agtagtagta gttcaaaaag atagcaaata tgcaaaagca
    17521 aaaagtttag cagatctttc tggagcaaag atcactgctc agttaaatac attccactat
    17581 actgtgatcg atcagatacc agatgtgcag aaacaacaag caatggataa tttctcagca
    17641 atgagaacag ctttagcttc tggaatgatc gatggttatg tcagcgaacg tccggaaggt
    17701 gtaacggcaa ctagtgtgaa caaagacttg gaaatgcttg aattcagcgg tgcagatggc
    17761 ttccagacaa acccggaaga tgttcagatt tctgttggta tgcgtaaagg cgacccggat
    17821 atccaaaaag taaaccagat tctttctggc atctctaacg atgaacgaac caaaattatg
    17881 gaccaagcag tcaaagatca gcctgctgca actgatagtg aagaaaaacc tggcatcatt
    17941 tctgatttca ctacgatttg gaaacaatac ggcaatatgt tcttacgagg tgctggactt
    18001 accttgttca tcgcattgat cgggacagtt gtcggtacta cacttggatt actgattggt
    18061 gtcttccgtt cggtacctga atcaggaaat gcactgactc gcttcttcca aaaaatcgga
    18121 aatgcacttc tttctatcta tgtcgaagta ttccgcggaa caccgatgat ggtacaagca
    18181 atggtcatct tctacggtct ggcactcgct tttggcattt cattgaaccg aacgattgcc
    18241 gctttgttta ttgtctctct caatactgga gcttacatga cagagatcgt tcgtgggggg
    18301 atttttgcag tagatgaagg gcagttcgaa gctgcgcaag ccattggtat gacccatggc
    18361 caaacgatga gaaaagttgt tattccacaa gttttacgga atatcttgcc agcaacagga
    18421 aatgagtttg ttatcaatat caaagataca gcagttttaa gtgtaatcgg cgtagctgat
    18481 ttgttcttcc aaggaaactc tgcttccggt gcgaatttcc aattcttcca aacatttacg
    18541 atcgtcggga tcatctactt agtcatgacc tttacgatca ccagaatcct gcgggcggta
    18601 gaaaagaaaa tcgatggtcc ttcagcttat accaagcttg aagaaatcga caattcaaat
    18661 ttgcaggaat aaggaggaag aatcatgaat gaaaaaatta ttgaaatcaa acatctaaga
    18721 aaaagcttcg gtgaaaatga agtattgaaa gatatcagcg taacagtcaa taaaggtgaa
    18781 gttgtgacga tcatcggttc ttccggttct ggtaaatcta ctttcttgcg ctgtatcaat
    18841 ttactggaaa aaccaacagg cggcgagatc atttataatg gagaaaatgt attagctcct
    18901 aaatataatt tacctaaata tcgaacgaat ctaggtatgg ttttccaatc cttcaacctg
    18961 ttcaacaata tgaatgtctt ggaaaactgt atgtctggtc aaacaactgt cctgaaacga
    19021 gataaagaaa ctgccaaaaa agtagcaatc gaaaatctgg aaaaagttgg tatggatcgt
    19081 tatatcgaag ccaaaccagc acaattatct ggtggacaga aacagcgtgt cgcaattgct
    19141 cgggcattat caatgaatcc agatgtattg ctattcgacg aacctacttc ggcgttagac
    19201 cctgaaatgg ttggcgaagt gcttaatacg atcaaagact tagcacatac tggcttgaca
    19261 atgcttatcg tgacgcatga gatggaattt gcaaaagatg tatctgaccg tgtcattttt
    19321 atggataaag gtgtgattgc agaagaaggt acaccagaag atatctttgt ccatccgaaa
    19381 gaagaacgta caaaagaatt cctttcacgt attttaaatg cttaggatca atagctgatt
    19441 gggacaaaga gctgcggtgt ttaccgcagt tcttttttat gaattactca taaaaggtaa
    19501 atttctgaa
//
LOCUS       DACCWV010000046        19388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCWV010000046 DACCWV010000000
VERSION     DACCWV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.046"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVW84_001373"
     CDS             352..1041
                     /locus_tag="IVW84_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917577.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVW84_001374"
     CDS             1028..2236
                     /locus_tag="IVW84_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7917578.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVW84_001375"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVW84_001375"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7917579.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVW84_001376"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVW84_001376"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7917580.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVW84_001377"
     CDS             complement(6339..7034)
                     /locus_tag="IVW84_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7917581.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVW84_001378"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVW84_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7917582.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVW84_001379"
     CDS             complement(8233..8424)
                     /locus_tag="IVW84_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7917583.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVW84_001380"
     CDS             complement(8445..9335)
                     /locus_tag="IVW84_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7917584.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVW84_001381"
     CDS             complement(9322..10089)
                     /locus_tag="IVW84_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7917585.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVW84_001382"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVW84_001382"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7917586.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVW84_001383"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVW84_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7917587.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVW84_001384"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVW84_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7917588.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVW84_001385"
     CDS             14705..15043
                     /locus_tag="IVW84_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917589.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVW84_001386"
     CDS             complement(15211..15969)
                     /locus_tag="IVW84_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7917590.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVW84_001387"
     CDS             complement(15987..16805)
                     /locus_tag="IVW84_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7917591.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVW84_001388"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVW84_001388"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7917592.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVW84_001389"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVW84_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7917593.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="IVW84_001390"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="IVW84_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7917594.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         5495 a         3985 c         3571 g         6337 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccac
//
LOCUS       DACCWV010000047        19178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCWV010000047 DACCWV010000000
VERSION     DACCWV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.047"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..1629)
                     /locus_tag="IVW84_001391"
     CDS             complement(61..1629)
                     /locus_tag="IVW84_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7917595.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1633..2043)
                     /locus_tag="IVW84_001392"
     CDS             complement(1633..2043)
                     /locus_tag="IVW84_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7917596.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2040..2963)
                     /locus_tag="IVW84_001393"
     CDS             complement(2040..2963)
                     /locus_tag="IVW84_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7917597.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2960..3838)
                     /locus_tag="IVW84_001394"
     CDS             complement(2960..3838)
                     /locus_tag="IVW84_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7917598.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3825..5162)
                     /locus_tag="IVW84_001395"
     CDS             complement(3825..5162)
                     /locus_tag="IVW84_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7917599.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5165..6268)
                     /locus_tag="IVW84_001396"
     CDS             complement(5165..6268)
                     /locus_tag="IVW84_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7917600.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6342..7850)
                     /locus_tag="IVW84_001397"
     CDS             complement(6342..7850)
                     /locus_tag="IVW84_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7917601.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7816..8508)
                     /locus_tag="IVW84_001398"
     CDS             complement(7816..8508)
                     /locus_tag="IVW84_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7917602.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8530..9504)
                     /locus_tag="IVW84_001399"
     CDS             complement(8530..9504)
                     /locus_tag="IVW84_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917603.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9804..10676
                     /locus_tag="IVW84_001400"
     CDS             9804..10676
                     /locus_tag="IVW84_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917604.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11258..13456)
                     /locus_tag="IVW84_001401"
     CDS             complement(11258..13456)
                     /locus_tag="IVW84_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917605.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13688..14680
                     /locus_tag="IVW84_001402"
     CDS             13688..14680
                     /locus_tag="IVW84_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917606.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14740..16161
                     /locus_tag="IVW84_001403"
     CDS             14740..16161
                     /locus_tag="IVW84_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7917607.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16154..17653
                     /locus_tag="IVW84_001404"
     CDS             16154..17653
                     /locus_tag="IVW84_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7917608.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17692..19086
                     /gene="uxaC"
                     /locus_tag="IVW84_001405"
     CDS             17692..19086
                     /gene="uxaC"
                     /locus_tag="IVW84_001405"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7917609.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5780 a         3903 c         3387 g         6108 t
ORIGIN      
        1 caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta aagaattaaa
       61 ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac ttcctctttt
      121 tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc ctgagagtat
      181 acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt ctttcaaatg
      241 aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg
      301 aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac gtaggcattt
      361 tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat ttaagtacag
      421 taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg tctccgcatc
      481 aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag attgaagaga
      541 ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc aatcagcatt
      601 tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg tatccataaa
      661 ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt cttccatcgg
      721 agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca cttcttgaat
      781 agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa gatatgtcat
      841 gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt gcaatgtaga
      901 agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt aaggaccagt
      961 aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc cttctgccgt
     1021 catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt tcgctaattc
     1081 tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg catcatattc
     1141 ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc atagttcatt
     1201 gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa agccgttact
     1261 atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga tcaaataatt
     1321 gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa gataaaacaa
     1381 tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat ttccttgcaa
     1441 agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat ttttcgactc
     1501 atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat accgctcttt
     1561 cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt tcactttgat
     1621 agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga atgccccagc
     1681 tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca acaatatgaa
     1741 tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag cagtcaaaaa
     1801 caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca gaccagtcat
     1861 gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta cagcaagcga
     1921 ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg ctaacccgcc
     1981 aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc attttctttt
     2041 catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat cgatgtttca
     2101 taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc atcccaatta
     2161 tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa gacatttgca
     2221 tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc agtcaaggta
     2281 cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc tgtgatgtat
     2341 ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc atctgcccca
     2401 ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg acgacaatac
     2461 tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac
     2521 ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa agatacgcga
     2581 tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc tgctgtattt
     2641 tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt agcacttcgg
     2701 aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt accggagata
     2761 actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat atattcatca
     2821 tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc tgccaaataa
     2881 tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt
     2941 atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa atatttctca
     3001 tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt tctggactac
     3061 ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct tcgtccaagc
     3121 acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt ttttcttgga
     3181 ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac ccatgatgat
     3241 ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga tcaatacaaa
     3301 ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc atagaaagac
     3361 caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc
     3421 tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa aattgtttga
     3481 acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa
     3541 cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag taaggagcac
     3601 catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga
     3661 ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact gtttccaatt
     3721 catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct cggtataatc
     3781 gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc aactgcataa
     3841 gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga ttggaatttc
     3901 tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg attttcaatc
     3961 gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg tctgctcatt
     4021 tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc catcgagaca
     4081 tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata aataaaacct
     4141 gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct gaatgctggt
     4201 gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt ccctcgcgga
     4261 ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat gactatattg
     4321 gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa tcgtattcct
     4381 cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact accactcatt
     4441 tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt gatcgtgata
     4501 ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc aatacagtca
     4561 tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc tggatcaatt
     4621 ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa cgttgcatta
     4681 ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat atctttcaca
     4741 gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc gagattatct
     4801 ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt accgaaacca
     4861 gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac ttttcgatgg
     4921 actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga gaatttcaga
     4981 accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc tcctgttaga
     5041 aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc atcaatggct
     5101 tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa aatatcatac
     5161 atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat
     5221 cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc tgatctgctc
     5281 cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct tttgtttcca
     5341 gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc cattcttctt
     5401 tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata ctactgccgg
     5461 aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac catgtcccgc
     5521 tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt tctttcagca
     5581 aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc atcaaatacc
     5641 agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca gcccatggtt
     5701 ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg aatagatgtt
     5761 tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca ctaaaattct
     5821 ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa atttgataag
     5881 gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt gccaactgct
     5941 gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg tacaataaaa
     6001 agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg tagccacgga
     6061 tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca aaataaattt
     6121 tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca tagtcataag
     6181 accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc atgatacttt
     6241 ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt aaaataggat
     6301 ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc cgctgctttg
     6361 tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca gtcttctaca
     6421 gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt agcatcaatg
     6481 acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa
     6541 ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa ttctttttca
     6601 gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga taacggttgc
     6661 acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc attcaattca
     6721 taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc taaaagatca
     6781 tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga tttgggaaat
     6841 gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc gccttcacca
     6901 tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg acccatcgtc
     6961 ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg tgcaaattgt
     7021 tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc attctcaaat
     7081 agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc tggagtgaat
     7141 ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt gaaaagttta
     7201 aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt gtcgtccaca
     7261 ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag ctcatccatg
     7321 gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac taagccagca
     7381 cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact cgcagcttcg
     7441 attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc tgatacatcc
     7501 cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat cgcaatttga
     7561 agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct ttcttctttt
     7621 gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc
     7681 acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt gattttagct
     7741 tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc ggcaagtgct
     7801 agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat aaattttctc
     7861 cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg agttcttcca
     7921 ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga agagactgcc
     7981 accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa tccctaccat
     8041 acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa atgccatttt
     8101 cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa tatagagtgc
     8161 tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc aattgtgtat
     8221 gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc tcatcagcaa
     8281 ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc
     8341 ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag gaccgaaagt
     8401 aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat aaaccaactg
     8461 taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat ctttccctct
     8521 ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat tagctaaaat
     8581 taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga aacttaactg
     8641 tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt
     8701 caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct gaccgatttt
     8761 tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat gccatgtttt
     8821 gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat cgactgcagt
     8881 tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca cttgtccgat
     8941 atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa atcccattgt
     9001 tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag taatcgaaac
     9061 aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa
     9121 tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg gaaaaaacac
     9181 gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga atgtcggttc
     9241 tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta ttcctaaaaa
     9301 tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc taaaaatcaa
     9361 gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt tgattcctgc
     9421 aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca gttgaaatgg
     9481 ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt ttgctgctta
     9541 tgacttcatg atagataact atctgaatta cgtatataat catttttacc gtaagtaaaa
     9601 acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa
     9661 taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat aggaaatgat
     9721 tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa catttaattt
     9781 attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa agaagtttta
     9841 cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt ccttttttaa
     9901 atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag tttatccttt
     9961 ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg accattttta
    10021 gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa
    10081 cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact
    10141 ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc ttagttgttc
    10201 gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct gttaatgcaa
    10261 tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg gaagaatcag
    10321 caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg ccattagctt
    10381 taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac acttatatga
    10441 ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt ttacgccaaa
    10501 tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg attccaccag
    10561 cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg attgtttatc
    10621 cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat
    10681 gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa taagaaggca
    10741 tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg aagaatctac
    10801 tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag cgatgataaa
    10861 aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa
    10921 tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc ttgaacgcca
    10981 tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg cgtgcttagc
    11041 cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc ttttattgct
    11101 tgaatcagtc cacaagaaaa acagcctctt gattaaacgg cgtttatctt gttgattctg
    11161 cggcaataaa atcagccagc agcaaaacat gaagtgctgt tttttgctgt cctcttttat
    11221 tgcttgaatc agatcacaag aaaaacagcc tcttgattca caaatatttt tttcgatatt
    11281 ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc ggatttcgat
    11341 attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac catttttttg
    11401 ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt tcctttttaa
    11461 atacattact taaatagtta gggttataat gcaacctatc agcgatcaca tctaaagaca
    11521 gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa tttttaaatt
    11581 catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt tctaaaacaa
    11641 gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt tgtttcatgt
    11701 tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt tcattgatca
    11761 agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa
    11821 tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct tcaaacaatc
    11881 gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca ggaacgattt
    11941 cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg gcttcttttg
    12001 cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg atgcctatac
    12061 tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa tactccatga
    12121 cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct tgcatttctt
    12181 cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg atcatgttgt
    12241 tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac agatcgattt
    12301 gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc tgcaaccgct
    12361 gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat agcgtttcca
    12421 gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct atgatccgac
    12481 tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat ttaagattca
    12541 gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag taggaaaaaa
    12601 gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc agattgcgga
    12661 tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta ttataatctg
    12721 atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga ttgtaatcta
    12781 gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga gtagtaaaaa
    12841 ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca acaacttgtt
    12901 ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt tcaaacattg
    12961 gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa ttatttttgt
    13021 tatttatgta cttctgttga tacttttcaa cttcctcttc ggtcagttga ctcaacgatc
    13081 cattgattac tccccattta ccagccaaac tgactaaatc ataattatta tcatccataa
    13141 caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag taatctgtat
    13201 atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac gtagaaaacg
    13261 aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg agcaaattct
    13321 ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa atgaataaac
    13381 taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt ttataaaata
    13441 ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt tcatttttct
    13501 gtaaaattga tactcatcta attaaatgag cattccccgc tttcaagaat agcataatta
    13561 aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa gatctattga
    13621 cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa
    13681 tgatggaatg gtaacgatca aagatatcgc taaagcagct ggcgtttctc atacgacagt
    13741 ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca
    13801 actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa
    13861 aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat caaatttcct
    13921 agcagattcc attgcaggca tccattcagt attagacaca aattacagtt tatctgttga
    13981 aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg gactgatcgt
    14041 tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa aagaatttcc
    14101 tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca ctgccgatga
    14161 tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata cgaagatcgc
    14221 ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag gggtattgaa
    14281 aagtctaaaa aaagcgagca tccctgtcaa tctagcctat tttttaacag gagattacag
    14341 cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc tgcctactct
    14401 ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac atttagctgg
    14461 cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag tcgtctccta
    14521 tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac
    14581 aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc
    14641 caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta attttatata
    14701 aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact gacaaaagaa
    14761 ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg caattttatg
    14821 cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt taaaggtaat
    14881 gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca gcaagataat
    14941 ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac ttccgagatt
    15001 atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta tctatctcta
    15061 gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg aatccaattc
    15121 gtggagactg ataatttaac agatactccc ccacagagct tcccgggcaa actgacagcc
    15181 tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc ttgcgaactg
    15241 attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga aaaatgggat
    15301 ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg tagtttagtt
    15361 gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca acggcataac
    15421 tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat cgaagggcct
    15481 aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat catcacagat
    15541 gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca tacagcgatg
    15601 gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa agatccttta
    15661 ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct ttctttacct
    15721 aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc
    15781 catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac ccgtctgctt
    15841 ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat cactgtttca
    15901 ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga tgatgaaaag
    15961 acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc tcgattattg
    16021 caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac agatacagtt
    16081 caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa
    16141 ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc gacgagacag
    16201 tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg atcagcaaac
    16261 gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg atttgaaagc
    16321 tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag acgtaactgc
    16381 cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat tggattatcg
    16441 atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat ttcaaggtta
    16501 cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac ctaccgttgg
    16561 gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac atccagatct
    16621 gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc agcttggaaa
    16681 agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca atgccggagg
    16741 agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca aaaaaatttt
    16801 aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt acgatgagat
    16861 cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg accagcgcat
    16921 atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc
    16981 gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg ctcaaggagg
    17041 cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat tgatggcacg
    17101 agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact tcaaacagta
    17161 tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata aagcaggtgg
    17221 gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt
    17281 cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc
    17341 tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc agttggtctt
    17401 gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca aggtatctac
    17461 gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag gcgtcttatt
    17521 agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag
    17581 tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt ttaaaaacgg
    17641 agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt tatgttttta
    17701 tctgatgatt ttttattaaa agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa
    17761 aacatgccca ttattgatta tcactgtcac ttagatccaa aagaaattta cgaaaacaac
    17821 aatttcagca atctgacaga agcttggtta ggaggcgatc attacaaatg gcgcttgatg
    17881 cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg cttctgattt tgaaaaattt
    17941 ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc cgctttacag ttggtcacat
    18001 cttgaattga agcgtttctt cggtattgac ttgttgataa atgaagaaaa tgcagagaaa
    18061 atttggacac aagcaaatga aattttggca gctcctgatt ttcgccgtcg ggaaatcgca
    18121 aaaaaatcga atgtagaagt gatttgtaca acagatgatc ccgcagacga cttgcactat
    18181 catcagttgc ttgctcatga agaaccagat ttgaaggttc taccttcatt ccgtccagat
    18241 aaagcactca atattgaaaa agaaggattt aatgcatggg tcacactact ggaagaagca
    18301 gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag tcttagggca gcggatagaa
    18361 tacttccatc aacatggatg tcgtgtttcc gaccacgctt tagacatctt acgctataaa
    18421 gaagcggatg aaacagtatt agaagagatt tttcaaaaag cacgagctaa caaactatta
    18481 tcttcagatg aaatcgatgc ttatcttaca gaaacactga tacatttgac ccacttctat
    18541 cattctcata attggacgat gcagctgcac atccatgctt atcgtaactg taatacacaa
    18601 atgttggaaa aattagggcc cgataccggc tatgatggaa tgaatgatct ttctttgact
    18661 attcctctgc aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc
    18721 ctttattcat tgaatccgaa tgactatcct gcgattttag ctttgatggg ctgtttccaa
    18781 aaagaacaga atggaaaact gcagttaggt tctggatggt ggtacaacga tactcgagct
    18841 ggtatgcgcg atcaattgac acaatttgct gatggtagtg ctttggggaa ttttgtcggc
    18901 atgctgacag attcaagaag tttcctgtcc tacactagac atgaatattt ccgtcgtgtt
    18961 ctttgtgaat ttttaggcga aatggtcatg cgaggagaag cacctgaaga tgaacaactt
    19021 ttaggaagtc tagttcaagc gattagttat acaaatgcta aaaattactt tggctttttt
    19081 gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc
    19141 cgtttatcca cgttttagag aagaagacgg accaaatt
//
LOCUS       DACCWV010000048        19017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCWV010000048 DACCWV010000000
VERSION     DACCWV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.048"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(240..1940)
                     /locus_tag="IVW84_001406"
     CDS             complement(240..1940)
                     /locus_tag="IVW84_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917610.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2447..3607
                     /locus_tag="IVW84_001407"
     CDS             2447..3607
                     /locus_tag="IVW84_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7917611.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3623..4066
                     /locus_tag="IVW84_001408"
     CDS             3623..4066
                     /locus_tag="IVW84_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7917612.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4549..5079)
                     /locus_tag="IVW84_001409"
     CDS             complement(4549..5079)
                     /locus_tag="IVW84_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7917613.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            5184..5735
                     /locus_tag="IVW84_001410"
     CDS             5184..5735
                     /locus_tag="IVW84_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7917614.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5888..6361)
                     /locus_tag="IVW84_001411"
     CDS             complement(5888..6361)
                     /locus_tag="IVW84_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7917615.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6499..7971
                     /locus_tag="IVW84_001412"
     CDS             6499..7971
                     /locus_tag="IVW84_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7917616.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7968..8678
                     /locus_tag="IVW84_001413"
     CDS             7968..8678
                     /locus_tag="IVW84_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7917617.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            9030..9314
                     /locus_tag="IVW84_001414"
     CDS             9030..9314
                     /locus_tag="IVW84_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7917618.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9631..10014)
                     /locus_tag="IVW84_001415"
     CDS             complement(9631..10014)
                     /locus_tag="IVW84_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7917619.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(10016..10600)
                     /locus_tag="IVW84_001416"
     CDS             complement(10016..10600)
                     /locus_tag="IVW84_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7917620.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10647..12122)
                     /locus_tag="IVW84_001417"
     CDS             complement(10647..12122)
                     /locus_tag="IVW84_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7917621.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12303..12614
                     /locus_tag="IVW84_001418"
     CDS             12303..12614
                     /locus_tag="IVW84_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917622.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12729..15065)
                     /locus_tag="IVW84_001419"
     CDS             complement(12729..15065)
                     /locus_tag="IVW84_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917623.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            15207..15875
                     /locus_tag="IVW84_001420"
     CDS             15207..15875
                     /locus_tag="IVW84_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917624.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15878..16921
                     /locus_tag="IVW84_001421"
     CDS             15878..16921
                     /locus_tag="IVW84_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7917625.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(17158..18039)
                     /locus_tag="IVW84_001422"
     CDS             complement(17158..18039)
                     /locus_tag="IVW84_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7917626.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            18198..18926
                     /locus_tag="IVW84_001423"
     CDS             18198..18926
                     /locus_tag="IVW84_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7917627.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
BASE COUNT         5911 a         3404 c         3782 g         5920 t
ORIGIN      
        1 tgaatggtca aatacaagaa aaggtatttg gtcagtctcc aaaagtcata ttcttcatcg
       61 atcattaaca gacaaagaac tggcccgcca aggatatgag gacatctcct tgaagtacca
      121 gttcgtacac tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg tggtgtgaga
      181 ggggcggaag gtcagttggc tttcccctct actcgatcgg atgtatttat cccttttgcc
      241 taagcacgta aaagcttgta tctatgataa ataaataaac atagcaaggt aaaccagcca
      301 atcaaagtaa gagaaagaat gaaatagaaa tagattggcg tttgataatg aagcgtgagc
      361 tcgttgattc ctttttgctg cttcacgatt ggggtcccga tatctgttac ttcataatca
      421 gtcaatgttt ttccattttg ctgtagcaca gtccgatcgt agacgataac agggatatga
      481 acagttttgt ccccagtgtt tttccacttg attgtcaaaa gaccgcctgg atgtgttttt
      541 ataaaagttg tttcgttttc tagtatttct tcgttatacc ggtcgtattt attgtctttt
      601 gtctccttat agattggcag atagtcaggt gtggtttttt ggaaaacgaa caagctggta
      661 gacatatctt tgctgaagaa tgttttcctt gcttcttccg gcgtatcgaa tagataagta
      721 tgtcgactga caaatgtttc attttcccac ttttccagat gctggtataa gttctggatc
      781 gtctgtgctg aaaaaataag aatgccaaat atacttaaaa gacggaacca cttttgttca
      841 gaccactggt tcagtgaaag ggcaaggtaa agtagaagca atagcgtaaa tggcacgaaa
      901 aatcgaaatg gaaactggat caaactaata aaaggcacat ttttttcgat caatgtcgtc
      961 catggaatca agctagtaga aagaaaaaag aaaacaagac ttgtgtacaa cacgattttt
     1021 tggaataagg tgttcttttg ttgaaagaaa ataaaaagca aaaaacctga taatatcaat
     1081 ataggaaagg ccttagggta atggagccaa taactgccat ccagatctat cgccttttga
     1141 ctcatttctc tgttaacaaa gggaggaaga atcttattcc cactgtaaac aatagctaat
     1201 cctgcccaaa tatttgctgt cagaacaaaa taaagcaaga ccgacttaat cagttgaagc
     1261 agtgcttttc ctttttcagt ctggttaaaa aagtaataaa gataaaaagg gatatacatc
     1321 aaagctaaca tgatcgaaga cagtaaatgg atctgtgtca taagtcccac tgagacagcg
     1381 actttgataa cagggaactt tttatgaagg acgaaatcag caatagggat catacataaa
     1441 ggataaaaac aagtccccca gctagtgaat ccttggttga tagaccagta ttgaatagaa
     1501 tacgtactca tgaataaaac ggctactggt acacttaccc aatttcgaac attgacatat
     1561 ctcagtaaaa tcagcataga aaatcctgaa ataagataaa gaatgaaatt tgaaagaatc
     1621 tgataatgaa accagctacc tgcaagcaac acgatcaatc cttgtaaata agcgaaaaat
     1681 gggccgtaaa cggcattcac gatcctgcca ctttgctgga atccataaag ggaaggaaaa
     1741 taatggaaat ccccatattt aatttgttgc gcggcatcat agaaccgatt gtaatggaat
     1801 ggggcatcag cacccaaaat tagtttatgt gtgaataaat gaggcatgag aaatgccaaa
     1861 gcaatgaaaa aaattgctaa aaatgggcca tattttttca attgtcgaac tcctttgcaa
     1921 actttagata aattcatcat atatcctatg cgagaggtag gcaagaaatt agaaaattca
     1981 tgaaaatatt tatgaatttt acaagaaaaa cataagtgaa agttctaagg agagaggatg
     2041 cgacataagt tttgttgcat ctccttgttt tgaaacagaa tttttcttaa tactcgaaac
     2101 aaagtagctt tttcatgacc tctcggtaaa cggtgttcaa tcagctgctt ctcggtaata
     2161 agctcactag cctagaaaat atgagaagct attttctgac tagcgctctt attactcaaa
     2221 gcataacgca gttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     2281 aataagccca acccaacaaa aaatatgagt agctattttt ttgggctgta cttattactt
     2341 gatgcaggac agctttttca caacctcatc ggctttcatt ttctattttt ttcctgaacc
     2401 catataataa aagaaagaac aaaaaaactt gcaagggagg acacatgtga agcagagtaa
     2461 gcaaaaacag tctcgtcgac ttttgtttcg tttgttttta ttaatatgta tcgtcgtagg
     2521 gttactgatc gctttatatc cattttatgt ggattcttta aatagtttta ttgatcaaaa
     2581 acgtatcgaa caagtacaaa aacggaccgc agctgaaatt gaagtgcagc gaaagaaaat
     2641 ggaggagaaa aaccaacgat taactgacca aggattcaat cctggtactg atcctttcaa
     2701 tgaaaagaac agaaatgaaa gtacaacatc cagccaatta gaggaatggc tgatcggaag
     2761 tatcaatata cctaagatcc aattaaatat ttcactttat gatcggctca atggtaggat
     2821 ccttgaaaat ggagcaggcg tcttgcaagg aacctctttc ccgcttggtg gaaattcgac
     2881 gcattcagtg atctctgctc acagcggttt acctaatcga cggttattta ccgaattaga
     2941 tcgtttagaa aacggagaca cgtttatttt gactgttttg ggggagaaat tggcttatca
     3001 agttaaaaat atacaagtgg tactgccaga tgacacgtct gttctaacga tttcagaagg
     3061 agaagactta gttaccttgc ttacatgtac gccgtatatg atcaacacgc atcgtttgct
     3121 agtgacagga catcggattc cttataacga atcggtaaaa aaagaagaag gaaaaggaaa
     3181 tcaggaaaga accatgcggc aatggtttat tcttgcagga acgataatag ctgtagtcat
     3241 actgttagtg tttatcggac gactaatcta tcagtataga ttaagtaaaa aagtgcttga
     3301 tttttcattt attattagtg attcgtcagg gaaaccggta aatggtagtt cattcatttt
     3361 gaaacataag aaaaaaacac ttactagaaa tggtgtgccg ttttcagtac aaagcgacca
     3421 tcatgggaaa gtcaagctgg atcaattgcc tggtggaact tatcgaatcg tttcagctga
     3481 tgatcctaaa gttgctgttt cttttggtat tagaaaagta aaacaagaga agatatactt
     3541 ttttgagggg cgaaaacttg taaaagagct tcaaaaaaat ggtttttatt ttaaaatcaa
     3601 tgaataagta aggagagaaa aaatgatccg cacaacaaca aaattgtctg ccaaagaact
     3661 ggaatctgtt ctatcgattt ggctgcatgc taacttggat tctcatgaat tcattccatc
     3721 aacttattgg gagaatcaag tagaatctgt taggaaagct ttaccagatg cagtgattta
     3781 cttagcagaa atagatcaag aaattattgg ttttgctggg gtgattgatc agtttatcgc
     3841 aggaatcttt gtgaaaaggg aataccggca acaaagaatc ggtacgcaac taatgaaaaa
     3901 aataaaagaa accaataaaa cactgacttt gacagtctat caaaaaaatc aatcagctat
     3961 tcatttctat ctttcacaag gttttacaat tcaaacacag caaaaagatg aagcagtaaa
     4021 tgaggaggaa cttgtgatga aatggaatca aaacgctgat ttttgatgaa cagaataacg
     4081 aaataatgct cccagcctaa ttttttgctg ggagcattat ttcattttga agtcactaat
     4141 ttaactgtgt gaatatgcag ttgttacaat ctttgataaa cggtgttcaa tcagctgacc
     4201 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccatt tgtttctcta
     4261 atactcaggt caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct
     4321 tctcggtaat aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct
     4381 tattactcaa agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg
     4441 accctcggta ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct
     4501 ctaatactca ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg
     4561 tttataattt tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg
     4621 ttcttcgcta ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc
     4681 aaattcagga acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc
     4741 tgcgatatgt tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg
     4801 gaaaaagatc attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt
     4861 ccctgttggc tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc
     4921 ttcttggtct tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt
     4981 atggtcattt ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg
     5041 agttccaata aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa
     5101 ttaaaacatg taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa
     5161 gatttgtcaa ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag
     5221 ggttattaag taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa
     5281 taggagaatt ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag
     5341 agcaaggaat cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt
     5401 atgaattaac agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg
     5461 aactaccggt taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg
     5521 atccggcatt agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc
     5581 atctattaga gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg
     5641 gacactacct gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa
     5701 cagatgtatt agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga
     5761 attcctttct atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg
     5821 ccttttttca agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta
     5881 gtggcgttta gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga
     5941 gcttgtaagt taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt
     6001 ctttgaagtt accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga
     6061 ttgcttttga aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt
     6121 ctgcagcttt tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta
     6181 tgactgtagg taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga
     6241 atcccttttg ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt
     6301 cactgatgcc aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca
     6361 tttttgtcac tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt
     6421 caagtaggtt gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat
     6481 tcagaaagaa ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat
     6541 aatcggacac ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag
     6601 accttattga cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat
     6661 gtgcaatggt taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca
     6721 tggctgatca ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg
     6781 atcggaacga ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg
     6841 atccaagctt gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt
     6901 ttcccgccag aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct
     6961 cctgctttag gccctacatt atctggttat atcattgata actaccattg gcgtgacttg
     7021 tttggtatgg tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa
     7081 agtgttattc cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt
     7141 ggattcggta gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg
     7201 aaagtaattg gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa
     7261 ttacatttgg aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt
     7321 gcagctgttt tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca
     7381 ttgtatattc aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg
     7441 ggtgcattga tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc
     7501 gcgaaacgtt tagccatcac agggatgttc attttgacag cggcaactgc accatttgct
     7561 ttcttgacaa aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt
     7621 gggatctcca tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac
     7681 ttgatcagtc atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt
     7741 acagctattt tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct
     7801 gtattggata atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt
     7861 taccatgccg cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt
     7921 ttattgaaga aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag
     7981 ttttgattgt cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc
     8041 aaaccttttt atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg
     8101 ctaatttgtc caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg
     8161 tttccagtgc tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa
     8221 aagaaaaagt gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta
     8281 cggataacga aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa
     8341 ctacttattg ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca
     8401 atcacgaata tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa
     8461 gtacggacca agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa
     8521 gtgaagcaaa agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa
     8581 tgagcaaagc agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag
     8641 cattcgcaaa attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgtaggag
     8701 gttgtgaaaa agctgctctg catcaagcag taagaacaat cagataaaaa tagcactcca
     8761 tatttttcat ctgattgggc ttagtgccgt agatgcaagc ttttgaacac cgtttaagga
     8821 agaggttgtg agcctgccgt ttaactctga gtcacaagga gcaatttcaa aaaacagctt
     8881 ctcatgtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat
     8941 tcgaaaaaag gaactgcgac aagacttttg tcacagttcc ttcttttttt tgctatgcta
     9001 tactagatgt aaaaaatagg agaaataaaa tgagggaaat ttattctttt gaatcaaaaa
     9061 tctatgcttc cgaagtcggc aagggcgggg catatattat ttttccgtat gatataagaa
     9121 aagaattcgg tgctggaaga gttaaagtcc aagcaacttt tgacaatgtg ccgtatgaag
     9181 gcagtatcgt gaatatgggc gtgaaaaatc cagatggttc agtctgctac atccttggac
     9241 tacgaaaaga tattcgtaaa aacatcggaa aagaaatcgg tgatattgta gcagttacag
     9301 tgaagcaaaa ataaaagagg ttgtgacaaa agacgtgtca caacctcttg ataaacggtg
     9361 ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga aacccatgtt
     9421 tacaaaattt ttcttactac tcgaaacgaa gcggctttat cacaacctct tgataaacgg
     9481 cgttcaatca gctgtccctc ggtattagct caaaaacggc aaaatatgag aagctatttt
     9541 tccatttttt tctctcatac tcaggtcata acgctgattt cacaacctct tctttcataa
     9601 tggtttttca ccatctttat aataaactgg ttatttgata tggaaatcta ttttttccaa
     9661 ccaatctaaa atcttgtaac ctaactcttc tgacatcgga aaagaggcgt tgatttgtgc
     9721 attggagata tggtcaacat ggataccgga agtgatcgta caacttcccg gaacggcagt
     9781 tttgatgatc acagctattt tttcggctaa gacatcgtct ttatggaacc gaccatcgtg
     9841 gctagggaaa cgtacagttt gcatctctgt atcacgagaa aatgtagtga ctgttccgat
     9901 atgtggtgta tctccgcctg ttaaagtgat caataagtct ttaccgatta ctttaacagc
     9961 agcttggatc gtgaaaccta gtttggttac ttcaactatc gtttcttttt ccatattaat
    10021 caccctgcca acgttttgaa tgctcattta caatttcaaa ttgatccaaa agtttcattg
    10081 tatggtgatt aacaagatct tggatcgttt gaggctgatt ataaaaagca ggaattggtg
    10141 gtatgatttg cacacctagt cgtgctaatt tagtcaagtt ttccaagtga atggcattta
    10201 atggcgtttc acgaggaaca atgactaact ttcgttgctc cttcattgtt acatcagcag
    10261 cacgtgcaat caggttgtcg cctaaaccaa cagagatacc tgcgactgtt ttcatacttg
    10321 ccggtacgat gatcattcca tcggtcaaaa atgatccgct ggcgacagca gcacctaaat
    10381 ctttgtttga ataatggtaa tcaaacaggc tttcgaattc agttagtgat aagtcggatt
    10441 ccagctttaa gttctgtttt gcccatggac tgatgattcc gtgtgtttct atcatcggat
    10501 attcttttag tttcttcact aggttgatgg cataaatggt gcctgaagcg ccacttacac
    10561 caatcacgat ttttttcttc cgttgtgcca attgttccat tttccgcact cctttttatg
    10621 atgtcaaaac agttgcagat cattcattat ttcaaatatt tttcccaatc ttctacttct
    10681 tggaattttg cacgtacaaa atgttctttc atatcgaaag gaactgtacc gtcaaagata
    10741 gtttttgcac ttatcccacg gaaacgaata gagccaggat tatattctgg taattctgat
    10801 gggtccaatg gatggttcct cattcctggg atcacgatga tatctttatc tccttggaaa
    10861 cgagtgttga aagtccaaac tacatcgttc atgtcgaaaa tatcaacatc ttcatcgact
    10921 aaaatgactg tcttcaattc tttgaaggct gataaagcaa gaatagctgc ttgacgctga
    10981 ataccttcat ccgcttcgtt ttctttacgg atttgcatga tggtcatcaa tttaccacca
    11041 cctgcaggag gattgtagac atttactact tttcctggaa tagctttatc gaccaaggca
    11101 agaatacttg cttctgtagg aataccggcc atagatacgt gttcttcact gggaccaatc
    11161 gttgtttgca tgattggatt gtcttttcgg tgagtaactg ctttgacttt cactacttgt
    11221 acagctggat tagcatcccc agtatagcca ggaaattctg gcattgcttt tcctgagttc
    11281 gtgttgatat cttcttgcat tgtttcattt ggcatgattt ctgcttcaat gatgaattca
    11341 gcacgagcaa tacccacttc atcgactgct ataccatcaa cgatttgaat aggttcgtta
    11401 cgcaaagctc ctgcacacca tagttcattg tagccaagtg gagtggtagg tggttcgaaa
    11461 gtcgtgccaa tggcaattgc tggatctaaa ccaatattga tagtgatagg cattggttta
    11521 ttcaaggctt cgaattgttt ttggaagtga ccaatgtggc gtccgccagg catgatgtac
    11581 ataccgatcg tatctttatc ttctagaacc atacgatgga tcgttacatc gctcattgtt
    11641 ttttctggat ctgatcctaa gactacaccc attgtgatgt atggaccagc atcttcagga
    11701 gtatttgtag gtgcaggcaa aatgttgcga atatcgaagt ctgcatcggt agctagatga
    11761 acgacttctt gtgctggagc gtctgctttg tctactttca caggtttgat tggattttct
    11821 acagcgtctt tcaagaaacg accaagtgtt cgataatcat ggtgcaaaat ttcacctaca
    11881 cgtttacgac tagccattgt tccgatgttc acacgtgtgc cggggaaacc tttgatattg
    11941 ttgaaagtca tagctggtcc ttcttgagtc ggacgtttta ctgtaccgcc agcaccgata
    12001 taacggtaaa caccagataa ttctgcttca ggatcgattt caatatctgt ttcgtgatat
    12061 tgacctggaa gtgtttttag ttcttcgatg acttttctta aatcatacgg ttgatctgtc
    12121 atgagagatt cctccttagt tgtctttacc tatctatagt aaaaggtgaa aaagaggaaa
    12181 acaattcaac ttgttcggta aagtattcta caatggaatg gatgcgtttt tttgataata
    12241 gaataaacta tgatatactt atcactaaaa gaaagcgcat acttagtgtt ggaggaagga
    12301 aagtgaaaaa aatggaggag tcatttccta aagcagtaaa agtggaaaat atagcgaata
    12361 ttttgaaagt tacatttgaa aacggagaag taaaatacgt gaaatctcat tggacagaag
    12421 agataaccga tgccttgcaa tttggaaaaa aaggaagagg aaaaagaaaa aatcttctcg
    12481 cactttctcg taatatgtgg attgggactg aagtaacgat tgaagcagat ggaacagtat
    12541 tcataaatgg aaaagacagg tatacgccag aagagttgtg gtacaaagga aagaaaagta
    12601 taccagaatt ataaaaaata attaaaaaaa acagcgcgaa acaaggaaca tattacttcc
    12661 tcgtttcttg ctgttttcta ctatttcagc gctcttatga ctcaaatcta aatgtggttg
    12721 tcacaacctt attctgcacg caaagcgata gcggcgtctt ttttcgctgc cattcgtgca
    12781 ggaagatgac cgccaagcat cgtcaatata gtagagacga tcactagaat aatcgcatgg
    12841 acaggattta gttgagcaac attttttaga tccgtcatgt tgtataagat gctattgata
    12901 gggaacgtag ctagccatgc aataaacaca cctaatatcc cagaagaaat acctaaaata
    12961 cacgtttctg cgtcaaatac gcgagtgatg tcttttttac gtgcacctaa agcttttagt
    13021 acaccgattt cttttgtccg ttcaatcaca gatgtataag taatgatact gatcatgatc
    13081 atacttgtta ctaaagagat ccctgcaaaa gcaataagga cataagtgat agcatccatc
    13141 aatccgccag tcagttcagt catcgttcca gctaaatcag tataaatgat tttatcttca
    13201 tctgatttgc ctttattgta atcatctaga taatccaaga tcttttcttt gtcttcaaaa
    13261 ttgttcggat aaatcatgat acttgaaggc aagctgtctc cacctaaata actaagcaat
    13321 gtttgtttgg cattttcgtc aactttttca gtagttaaga catttacacc actgtctctt
    13381 tgagctttga cgatttctga ttctttgttt tcgttgacga tttgagtcgt taattgatcg
    13441 ctataagcga ttcctggaga taagagattc attgtagaag aagatttcac gcgtaggatc
    13501 cccgagatct tcaattcatc agaagcattc tgatacatcg catcataatc tgtattaggg
    13561 ataaaattcc cagttggaag ttttgtatag taagtattgt tgtttactaa tttgaatgtt
    13621 gtaccaacga tatcatcaaa atcaagtttt tcgtcttctt tcacatcaaa tccaagattc
    13681 ttcaatgcgt tgatattagt attattattg ccgtctacga tcagcacgac atctgttgct
    13741 gaggctggat aacttccggc aagtaaagaa taattgtctt ttaaaaagtt ttctttgcta
    13801 gtgtcaagct gtgtagggaa acttgacact cccacacctg tcattgctga catcgtacta
    13861 gataaagaaa gagattccgt atcaggattt tgattcgaga agctgacagg ctgtactttg
    13921 ccatttacat cacgtaaaag attgattcct gtagtacggg taaatccgat attattactt
    13981 aagttaggat cgatgtctgt tacgtaatcg acgtatgttt gatcaatctt attcgtgtgc
    14041 tgtgctttat cttcttcgct gacttttgca gtaatcgttt tgctgtctgg ataatccgct
    14101 ttgcttgcac ccaatccggc atcgtcacga gtctggttag tcgttacctt agaaatagta
    14161 atcggaaatt tcgccatcgt ttcagcttgc gtgttatcga tttgtttttg gaatccagaa
    14221 gaaagggaca gaacgatcgc gattccgatg atcccaatac tggaagcaaa ggaggtcaag
    14281 aaagtacgtc ctttttttgt tcggatattg ttgaaggaaa gctttaacgc tgtccaaaaa
    14341 ctcatctttg ttctgcgtaa gttaaattga tcatcttttg gtcgttcgat gtgaggatgg
    14401 gaatctgtta agatcttacc atcagaaaat tcaatgattc gatctgcgta ttgatgggcc
    14461 agttcaggat tatgtgtgac catgatcact aatttttctt tcgataactc ttggatcaat
    14521 tccatgatct ggatacttgt ttctgtatct aacgctccgg ttggttcatc gcacaaaagg
    14581 atatctgggt cattggctag tgctcgagcg atggccactc gctgcatttg tccaccagat
    14641 aactgatttg gctttttatg catatgattt gtcaagccga ctcggtgcaa tgcatcttct
    14701 gcccgttttc gtttttcgtc tttagaaaca ccacttaatg tcatcccaag ttcaacgttt
    14761 tcaataatgc ctagatgacc aatcaagttg taactttgaa aaatgaaacc gatagagttg
    14821 tttcggtagg catcccagtc actgtcctta aagtctttag tagactttcc gttgattacc
    14881 atatcaccgg aatcataatt atctagtcca ccaatcacat tcagcatagt cgttttacca
    14941 gaaccactag gacctaaaat cgctacgaat tctttttgtc tgaaagcgac agacacacca
    15001 tccaatgctt tggtggtggt ttcgccgact ttataatatt tttttatatc ttttaattga
    15061 agcataatca cccttctccc gtattgaaat attcggtata ctaataagta tatcagaaca
    15121 atgatcgtag tatacgaaat aaatgttaac tgaagatgaa ctacaagaaa aaatctagaa
    15181 atttttcaaa aaacagaggg agatcaatga atcgtatttt agtagtagaa gatgatccgc
    15241 aattacagtt gctttaccgt tctgtgttgg aacgagcggg cttttcagtc atcaccgcat
    15301 tgaatggaga ggatgcgctg aaaaaactag aagcgacacg tatagaatta atcattacag
    15361 atatcatgat gccgaaaatg gatggctatg acttgctaga ctcattacgt agcaccagat
    15421 ttgacacacc tgttctaatg attacggcaa aggccgattt tgaggataag aaacgcgggt
    15481 tcaaattagg agcagatgat tatatgacaa aacccatcga tgtcaatgag atgatcctac
    15541 gtgtggaagc attgcttcga cgttcaaaga tcaattacag cagtagcttg ataatcggac
    15601 aaacaaaatt agagctagat gcttatacag tgttacaaga tcagcattcg ttgattctgc
    15661 ctcaaaaaga atttctgctt ctgtataaat tactgtctta tcccaacaag atcttcactc
    15721 gccaacagtt gatggatgat atttggggat tagatacgaa tacagaagaa cgaacgatcg
    15781 atgtacatat caaacgttta aggacccgtt ttgaaaagac ggaagatttt caaatcatca
    15841 cagtacgggg actaggctat aaggcggtta tttaaagatg aaaaaaaagc tattttcaca
    15901 attatggatc tattttgcta gtattgtctt catttcgatc ttagtgacac tgctgtgttt
    15961 tctcggtttt attttcttat tgtctcgaca ttctatctcg cctgctgaat cggctggaaa
    16021 accaacgtta tacccgttat tcgtctttgc ggggttcagt atggtcgtgg gtacaggaat
    16081 atcgattttt gttgggcggc gtatcctgca tccaatcagt gcgctaggga caaatatgag
    16141 tctggtagca acgggagatt tttcgattcg aatggatgaa cagcagaaag tagcagaagt
    16201 acagcaactg tataaagatt tcaacgttat ggtccaagaa ctcaatagta tcgagacact
    16261 gaggaatgac tttgtgtcca gtgtatccca tgaattcaaa acaccactag caacaatcca
    16321 aggatatgtt caattgttac aagccccaaa tctctctgac gaagaacgcc aaatattttt
    16381 atatcggatt atcgaaagta tcacgcagct ttcacaatta acagagaaca tcttaaaatt
    16441 gaacaaactg gaaaaccagc gtatccaact ggaaaagaaa gagtatcgat tagatgaaca
    16501 aatcagagaa gtaatcgttt tttttcagcc taaatgggag aaagaacagc tagaattgga
    16561 tatcgaactt gctgcagtaa attacacagg caatgaagag tttttgtacc aagtgtggct
    16621 gaatatcatg gataacgcca taaagtacaa tcaaatcaat ggacagattc atatcaaact
    16681 atttgagaca gccacagaga ttgtactgga agtgaccgat tcaggtgtag gaatgaatga
    16741 agagaccaga gatcgaatgt ttgaaaaatt ttatcaagga gatacaagtc gtcaaatttc
    16801 tgggaatggg ctaggacttt cactggttaa aaaaattcta gaactgcatg atggacgtat
    16861 tgattatagc agtatcgaag gagtaggaac gacagcaatg atacggttaa gtaaacaata
    16921 ggaaaaagaa taaaacccct aaaaattcta ttgaaggttt ttaggggttt ttagtcgttt
    16981 ttttgtgatt agggaaatgt tcttacggtt cttttggcat aaaaatcgct gctttcttat
    17041 tgaactacga gaaatgttag aggtgtcatt gaaacaagga catgatgcaa gaaagagagc
    17101 gaaagtcatg cgtcgctctc tttttttggg tggatagatg gacagaaaat gtagggctta
    17161 gttttcttct tgatttaaat actcccaaaa tgtatctgaa gccaaagaat gctcaccgac
    17221 tttacgagta aatgcaagat aactctctgt tgttggagaa atagggagaa tcacagtatt
    17281 ttcttttaat agattcttga ctgttttttc cataacaata gcaatcccca tgtcatttgc
    17341 aatcatattg atgatcaagt cgattcttgc tcctttataa gagatttttg gttcaaatcc
    17401 agcttcgcga cacagatcta gtacaggttg atacaagttc gtagtggggc caaggagcaa
    17461 aaagttctcc tttttcagtt cgcttagatc caatttcttt ttactagcta aaggatgatt
    17521 tttaggtaga actgctacaa aatcgtctgt ttcagtaggc aaccactcaa tcgaacttgg
    17581 tggggcctgg aaacttctag caaagtggat cgtgtcacta tgttcattgg cgtcattgta
    17641 agttaattcg acactttcca cttctttcaa ctggactgta aattcaggat ggcgtttcaa
    17701 aaactcggtg attttggaaa aacctttata attgatcatt gtcggtatcg ttaagatatt
    17761 taatgaaaga ttgttgtctt tcgtataggt ttcaagcact tcttgcatag catggtattg
    17821 ttctacgaca ttttttacat atgggagcaa taagcttcca gcttcggtca attcgatttt
    17881 tctatgggcg cgttcgaata atttgacgtc taattctgtt tctaacgaaa gaatctgttt
    17941 agaaatattt ccttgggtgg tgaattgtcg atcagcagtt ttggtgaaat tcattgtttc
    18001 ggctaaatca agaaaaactt cgaatttatg gatatccatc atattcctcc tacttattcc
    18061 aataaagaat actttaacac aaaaaatgaa ttggtgtaat cttctctgtc tgttacaatc
    18121 tttcttgcag cgaaagcaat aattacagaa atttttctga tttttttcgc ttcgtagaaa
    18181 aagagaggag ttttttgatg ccaactggag ttttgatcaa tgtaggttcc gttttgtttg
    18241 gcggactaat tggcggatta gtaggaaata aattaagtga acattttaaa gcacagctga
    18301 cgatggtttt tggtgtgtgt tcgatgggga tgggaatcta ttccattgcg ccgatgaaaa
    18361 atatgccggc tgttattttt gctttagtga taggaacagc gattggtttg attgttcatt
    18421 taggtaatgg gataaataag ggggctgcgc tgatgcaagt acctatttct aaaatcttcc
    18481 ctagtgaaaa gcttggtatg acccacgatg aatttatcag cacattagtg acggtcattg
    18541 ttctattttg tgcaagtggt acggggatct atggaagttt agattctggg atgacaggag
    18601 acagtacgat tttgatttct aaatctgtcc ttgatttttt cacagcagct atttttgcgt
    18661 gtaatttagg gtatgtcgtt tctgtgatag caattcccca attcattatt ttttatattt
    18721 tgttcttatt ggcaaaattt atttatccat tgactacacc agacatgatc ttagatttta
    18781 aagcttgcgg cggattcttg atggtcgcta ccggttttag aatgatcaac gtgaaaatgt
    18841 tcccagtagc tgacatgata cctgcaatga tcgttatcat gccactgagc tggatgtgga
    18901 caaattggat tatgccactg ttataataaa aatcaaagag aagccatttt ttgctgacta
    18961 aaaaggatca aatcttttag tcagtttttt tgtatttgac aaactgattt tgaaact
//
LOCUS       DACCWV010000049        18750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCWV010000049 DACCWV010000000
VERSION     DACCWV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.049"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            10..1149
                     /locus_tag="IVW84_001424"
     CDS             10..1149
                     /locus_tag="IVW84_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7917628.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1142..1942
                     /locus_tag="IVW84_001425"
     CDS             1142..1942
                     /locus_tag="IVW84_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7917629.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1942..2769
                     /locus_tag="IVW84_001426"
     CDS             1942..2769
                     /locus_tag="IVW84_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7917630.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2747..3481
                     /locus_tag="IVW84_001427"
     CDS             2747..3481
                     /locus_tag="IVW84_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7917631.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3581..4447
                     /gene="rfbA"
                     /locus_tag="IVW84_001428"
     CDS             3581..4447
                     /gene="rfbA"
                     /locus_tag="IVW84_001428"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7917632.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4461..5033
                     /gene="rfbC"
                     /locus_tag="IVW84_001429"
     CDS             4461..5033
                     /gene="rfbC"
                     /locus_tag="IVW84_001429"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7917633.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5055..6083
                     /gene="rfbB"
                     /locus_tag="IVW84_001430"
     CDS             5055..6083
                     /gene="rfbB"
                     /locus_tag="IVW84_001430"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7917634.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6181..7032
                     /gene="rfbD"
                     /locus_tag="IVW84_001431"
     CDS             6181..7032
                     /gene="rfbD"
                     /locus_tag="IVW84_001431"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7917635.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7066..9099
                     /locus_tag="IVW84_001432"
     CDS             7066..9099
                     /locus_tag="IVW84_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917636.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9143..10423
                     /locus_tag="IVW84_001433"
     CDS             9143..10423
                     /locus_tag="IVW84_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7917637.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10633..11439
                     /locus_tag="IVW84_001434"
     CDS             10633..11439
                     /locus_tag="IVW84_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917638.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11451..12671
                     /locus_tag="IVW84_001435"
     CDS             11451..12671
                     /locus_tag="IVW84_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917639.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12661..14247
                     /locus_tag="IVW84_001436"
     CDS             12661..14247
                     /locus_tag="IVW84_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7917640.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14286..16424
                     /locus_tag="IVW84_001437"
     CDS             14286..16424
                     /locus_tag="IVW84_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7917641.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(16792..17775)
                     /locus_tag="IVW84_001438"
     CDS             complement(16792..17775)
                     /locus_tag="IVW84_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7917642.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18153..>18750
                     /locus_tag="IVW84_001439"
     CDS             18153..>18750
                     /locus_tag="IVW84_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7917643.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETE"
BASE COUNT         5931 a         3125 c         3900 g         5794 t
ORIGIN      
        1 ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta
       61 tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa
      121 ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt
      181 atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt
      241 gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat
      301 gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt
      361 gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc
      421 tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg
      481 attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga
      541 atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt
      601 cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta
      661 ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg
      721 aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca
      781 gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat
      841 aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg
      901 acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga
      961 agaatcggaa cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa
     1021 ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg
     1081 aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata
     1141 gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa
     1201 aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat
     1261 ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa
     1321 atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa
     1381 tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt
     1441 ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat
     1501 gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct
     1561 ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga
     1621 caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg
     1681 gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt
     1741 tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc
     1801 aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc
     1861 gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa
     1921 gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag
     1981 caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa
     2041 attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa
     2101 gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt
     2161 gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg
     2221 ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct
     2281 gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat
     2341 atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca
     2401 atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct
     2461 gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa
     2521 actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa
     2581 gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat
     2641 gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc taaacagttc
     2701 ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg
     2761 aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag
     2821 agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga
     2881 tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg
     2941 aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca
     3001 gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa
     3061 agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag tagtttctga
     3121 tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa
     3181 ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc
     3241 tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga
     3301 tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct
     3361 gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa
     3421 aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg
     3481 ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga tgagataata
     3541 acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc
     3601 tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc
     3661 gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa
     3721 agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa
     3781 cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc
     3841 acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg
     3901 ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga
     3961 tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga
     4021 atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa
     4081 ttacgctgta actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat
     4141 caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa
     4201 aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca
     4261 tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa
     4321 agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct
     4381 ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc
     4441 tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg
     4501 tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa
     4561 aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg
     4621 aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga
     4681 ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga
     4741 cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac
     4801 ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt
     4861 gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag
     4921 cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc
     4981 cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa
     5041 ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta
     5101 atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac
     5161 tgacttatgc aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg
     5221 tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag
     5281 ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg
     5341 tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac
     5401 gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc
     5461 caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa
     5521 gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt
     5581 ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg
     5641 aaaaatttat tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg
     5701 gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc
     5761 tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag
     5821 ataataagac cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg
     5881 accatgtaaa cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac
     5941 gggaagagtt aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga
     6001 tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag
     6061 catatgccaa aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca
     6121 tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat
     6181 atgtttttat taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat
     6241 gaaaaaaatc tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag
     6301 aaaacaatgg cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac
     6361 acagcagtag ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat
     6421 ggtacaagaa atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt
     6481 acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg
     6541 aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt
     6601 ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc
     6661 gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag
     6721 tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa
     6781 aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc
     6841 gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt
     6901 ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta
     6961 ggatttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa
     7021 atgaataagt aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca
     7081 agaagtgcac gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt
     7141 gacctgtttc taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact
     7201 tttaatttaa acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat
     7261 gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg
     7321 gtgcagacac ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat
     7381 tattcattaa acggacaaaa catgatcatt gcgtataatt ctcctgtaaa ggatattacg
     7441 gttatcggaa aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc
     7501 ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata
     7561 ttaacgaaga gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca
     7621 gcagtccagt ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg
     7681 atgactgctt tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg
     7741 atgcttttgg tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa
     7801 gtgatgctgg cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag
     7861 aaagtttcat gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc
     7921 ggagtcatgg ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc
     7981 ttatatccag gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct
     8041 ttccttacaa attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg
     8101 gccctgttct atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc
     8161 aaaaaagata gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct
     8221 atcttttgga ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat
     8281 gttcctccat taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg
     8341 tttatcgctt atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg
     8401 gcagtaaatg catggtctta tttctatgcg attacgcatt cgaaaatgga taattatttt
     8461 tcggattttg aaaccatcgc agttgctgtg ttgggaacag tgattttagc ttttgcatta
     8521 tttggctgga gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt
     8581 ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa
     8641 atccaaaaaa tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat
     8701 aacttcaccc caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata
     8761 gctgcatgga agaaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct
     8821 catacaagag cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg
     8881 ttttcggtca cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt
     8941 aaacgaccat taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga
     9001 ccggataaag acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca
     9061 caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc
     9121 tgaaatgtta gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa
     9181 caaaatgagt gtcggtttct tgcttttgtt actgacgcta gtcttttttg tttggacagc
     9241 agggttaggt tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt
     9301 tagtgtggct attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta
     9361 tctaggtgtt gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga
     9421 gtctttattt ggagacttac tgtttccttt gattatttta ttttgtttag taattaaatg
     9481 gatcgagtgg acaaaatggg atcgcggtat ttatctgatt gtcttttcgg tcttcctcgg
     9541 aatcacgtta tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc
     9601 tccaggcaat tcgctttctg atatttggat caatacgaat ataatcggaa gttctttgat
     9661 gactcttgct ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat
     9721 catcggtgca gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg
     9781 tgcattagtc gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg
     9841 tgtcattcgt gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc
     9901 ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca
     9961 taaattctat cagacgctag gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt
    10021 tatcttccaa cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa
    10081 tatctatgga ttgataggat ttgggattgc tgctttgctg cttatccttt ttattggtcg
    10141 cttaacgttg aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc
    10201 ggtgatgatg cagtcattta tggaggatac attgacttca tacccatggg ttccgattat
    10261 ttatatcctg ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt
    10321 ctatgttatg gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca
    10381 accagatagc gctcattctt ccaggatcca acgctatcat tgataggcac aaaatacgag
    10441 agaggctgtg acaaccgtcg cagccttttt tggtttattg caattgtcta gcatcttctg
    10501 taaaatagaa acgttagaga aaatcttgta tcatagaaaa ttaagaaaga aataagaacg
    10561 cagctcggta ccaaaaatat ggtaaaatgg cacttgactg taattcactg aaaatcgctt
    10621 ggaggaacac aaatgtttaa agatttgtat ttatttatga aggacattta tcagaacaga
    10681 aaattattgc tacagttttc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta
    10741 ggaatcttat gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt
    10801 ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt
    10861 accgggatga ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt
    10921 cgggaatata cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg
    10981 aaaatcctat ccaatctgta cacccactta ttcttcattc tgattggcat agctgtcgcg
    11041 cttataaatg gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc
    11101 atgtgtgctt tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct
    11161 gatatcatgc aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta
    11221 tggagcccta atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac
    11281 tatgttgttc aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga
    11341 acatatactt tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg
    11401 ttccgtagat tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat
    11461 atgcaattag actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc
    11521 gatttcgtga agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga
    11581 aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat
    11641 cgggaaaatc tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga
    11701 tggaaatcga aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt
    11761 attctggcta tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag
    11821 tcgatgaaat ggtcgatgat atcattgaat ttgctgacat cggagaccat atttatcaac
    11881 ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg
    11941 tgaatcccga tattttgatt gtggatgaag cattggcagt aggcgatctt gaatttcaac
    12001 taaaatgtat ggaaaaattt acagaaatca agaactcggg aaagacgatt ttattcgttt
    12061 ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc ctattggttg aaaaacggag
    12121 aagtcgtcga atatggcgat acgatggaag taacgacgaa ttatgaaaac tttttaaaga
    12181 aaaaatcagt taaaacagtg gatcggaata aaaaccaagt agaacggttc gccgctccag
    12241 atatcgtcga cgtgacgaaa gcggaattgt tggataaaga caaaaatccg gtggatatgc
    12301 tggaacaagg agaaaaacta tacgttaaag taacttatga agtaaaagat gatacaatca
    12361 aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa tttttatgta tgcggactta
    12421 atactctact agatggtatc aagattcctt ggaaaaaagg agagaatatc ttttatctgg
    12481 agtatgaaaa gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag
    12541 aaaatgcaac ggttcctttg gtatataaga caaagtacat gaacttgttt gtcagcggtt
    12601 cttatatcgg agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca
    12661 atgaaatatg attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca
    12721 cagatcaaac caggaagtaa agttttagag tttggtcccg gaaacggccg attaacgaaa
    12781 catttaatcg gagcaaaaca atgtgaagtg agcattgttg agttagataa ggaattgttc
    12841 gattttgtca gcgaatttgc tcaagatgga ttttatggtg atatcgaaag ttttgagtgg
    12901 gcaaactatt atgctggaca gacatttgat tacgtcttgt ttgcggatgt tttagaacat
    12961 ttggtagatc ctggaaaaac attgaagaaa gtcagagaat ttttgaatga agaaggagag
    13021 atcctgatta cattccctaa cttggcgcat aattctgtca tgatcgattt gttcaataac
    13081 caattgccgt gggcttctta tggtctccta gatgaaaccc ataattcttt ttatactcat
    13141 gatggcttcc aaaaagtttt tgaaaaagcg ggattattca ttaatattga agattatttg
    13201 tacttagctg taggtgatac agaattaaaa tcaacttatg aagaattgcc ggaagctgta
    13261 cgctatgatt tcaaaatgcg accatttggt gaagtctacc aatacttttt ctcattgatg
    13321 aaacatccag tagcacaaag ttccattgca gagccacaaa attctaatta tgtaaaagtg
    13381 ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc aacaaatacc attcaataat
    13441 ttcacagggg aaaatgagac actaagtttc cccgtatcag aaacaacaga aagaatggtg
    13501 tttcgcttcg cgcaacaacc tagctttatc gaattcagcg cagaagctgc cggagaaaaa
    13561 ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat
    13621 gggaaagaat taccagagtt cgttctaacg gatatttctg ggaaagaagt gactatccat
    13681 tgtcactatc gctttatcgg ggaattgaca ccaacaatga aagaattgtt ggaaacgatt
    13741 cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa atgacgaatt agaaagagaa
    13801 aatcatcatc ttcttgagga aaatacaaga ctagaccata cgttaaaaac aaccacaaat
    13861 cgctattgca cattgttgga aagtgatgaa tggacaataa aacatagact aggacgacgt
    13921 aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt ctatatgtat tgacgagaaa
    13981 atctgggatg cagaaacaaa aatattaaaa atcatcggat ggggaatcgc taattcggat
    14041 cgccagccct tgtcctataa gttatcagct gaccaatcgc catttttcga agcaattcct
    14101 gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa aaggaacaga agcgggattt
    14161 gagttacgaa tcctttgcga gcaagaacgt tcattcttag tggaagcagt agctcaaaat
    14221 ggccaatcat ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac
    14281 gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca
    14341 ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga
    14401 taaaggtaga aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca
    14461 atcatttata taatcttgat gttaagaccc aaagcggttt taaggtacgt ttatcaggga
    14521 aaatgagagg aaaagccatc ttagacttcc aaacagcgag acatcaaaat ggcatagctg
    14581 tgaaattaaa cggtaaatac ccttatgatg acggcattga atcttcatgg gaaagaaaaa
    14641 aaagattact gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaagcgatcc
    14701 gtcgggtcaa attggaattg aagccaggat cgatcgacta tgcagagtgg atcagtcgtc
    14761 atgagaaacc agatttgaaa gagcaaagga aagaagcaca aggatttgcg taccgtccat
    14821 tgatttccat acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg
    14881 attcggtact ggatcaaacg tatgatcatt gggaactatg tatcagtgat gatgcttcta
    14941 ctgatccagc tatccgaaag tgtctggaat cctatcaagc aaaagatgat agaatcaaag
    15001 tcgttttccg aaaagaaaat ggacacatca gtctggcaac taattctgca ttggaaatag
    15061 cagaaggaga atttatagca ctcttagata atgatgatga attaccgcca tttgcattat
    15121 atgaagtggc aaaagtgcta aacgtacatc cagaactgga tgtgatctac agtgacgaag
    15181 ataaaatcga tgcagatggc aatcgctttg atccgcattt caaagcagac tggtcgcctg
    15241 atacattgat gggaaataat tatatctctc acttaggcgt atatcgaaca agcattgtta
    15301 aagaacttgg cggtttccgt aaaggctatg aaggttctca agattatgat ttggtccttc
    15361 gtgtgacaga acaaatccct gcagatcata tttaccacat tgaccgagtc ttgtatcatt
    15421 ggcggacgat cccaggatca actgcaagta atggcgaagc caaaagttat atctatgatt
    15481 ccggagtgaa agcattgaca gatgccctgt ctagaagaaa catcaaagga agcgtacgtc
    15541 ctggcagaat ttctggattt tatgagattg cttatgacgt cttgcaagag gatttggtca
    15601 gtgtgatcat tccaacgaag aatggctatg aagatctaaa aacttgtgtg gattcgatca
    15661 tcgaaaaaac gtcttattca aattatgaaa tcattattgc ggataacgga agtacagatc
    15721 caaaaatgca agaattattt gcagaataca agcatcagct gaaagaccgc tttatcgtag
    15781 aattgattga cattccattc aattattctc gaattaacaa tttagcagca gaaaaagcaa
    15841 acggcaaata ctttttattc ttgaataacg atacagaagt gattgagcca gattggatga
    15901 cggtgatggt ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt
    15961 atccagatga tacaacgcag catgcaggag tattattagg aatcggtggt gttgcagggc
    16021 atgcattgaa taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg
    16081 tcaattactt agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg
    16141 ttaacgggtt tgatgaaaca ttagaagtag cattcaacga tgttgacttg tgcttgaaag
    16201 tttatgaact gggtcgttac aatgtctatg cacatcaagc agaattgtac catttcgaat
    16261 ctaaatcaag aggttatgaa gatactccag aaaaacaaag acgttttgct ggtgaaatca
    16321 aaaaaatgca agataaatgg ccagcatata ttgcgcatga tccattttac aatgataatt
    16381 taacaaaaga aggaatcggc gacttttcgt tgagaccaga ttaaactata ataaaaagct
    16441 gttatgttgt gattcgctcg cacataacgg ctttttatag ttttttgtca aaaaaaggca
    16501 gaaggtgccg actcaaaaaa ataagtcgag gttgggacag aagtgtttaa ctccgagaaa
    16561 taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttaagaa
    16621 gaagttgctt ctgctcccac cgtttattcg tttttaaaga atgagacaaa agtgtttctt
    16681 acttttgtcc cacgctctta ttcatgctca gagcgccaat agatacaaaa tcatagccaa
    16741 agtttcacga accctagctg tcaaagaatc gatattttat tttaatatag gctattttaa
    16801 aataaatggt ttaaggtatt cctctacaaa agccactcta tcaattatca tgggtttaaa
    16861 ataagaagtg atggagataa ccagattgtt cttagcgtca atataaagaa catttccact
    16921 attaccaatt gctgaatata tttcgttctc agaatctaat acccaccata aatagccata
    16981 tctcatattt tgtaagtctt ctaaagcact ggttctaggt ttgaacatct cgtttatcca
    17041 catgggtggt acgatttgtt ttcctttata catcccttta tttaggcata atacaccaat
    17101 ttttgccaga tcacttgccg ataaacacaa accatatcct gccgtcccga tatttagtgg
    17161 atcacaaaac cagacattgg ttttaggaga cttatccatt gtaaactgct tatgttcttc
    17221 agctgtttca gcataataat tgacatggtt tggtatccct ataggatcaa atagatactt
    17281 attggcaaaa tcaacagttg ttagcttact tgtctgatac aacaagccag ttaatatgtg
    17341 taaacagaca gtttgatagt tgaattcgcc ggttatacct tttcttcctc ccaataaatc
    17401 tagtgatgtt tttgtccaat cttcacttga acagactttt gtccatggat cgcctttaca
    17461 tttgtagggt gctttcattg ttagcaagtg cttgattgta atagcgtaaa tagttttctc
    17521 cccgcgtttc actttatact ctggaaaata atctaatact ttatcatcga tacttttgat
    17581 taatccttgc tctaaagcaa tgccaatcag caaagataca atacttttcg ttgctgacat
    17641 gatatgacaa gtatcgtctt ttttataacc attccattca tctgtataaa cttctacccc
    17701 atccttatat gcgacaattt gacatatatt tggttgtcga aaaccaataa aatcatgcaa
    17761 ctcttgttta ttcataagat attacctcac tttcttctat aagcataatg tcattaaaaa
    17821 tcgatttcaa ttttttcgat tcttcacgta atacaggtat tcttatggac tagacagcgc
    17881 tttgggaaga tggattctct gaaaaatata aaggttcact ttatgagtgg ataaaaaaag
    17941 cagcaaatac ttcgacttct ttaatttacc aacattagtg aaaatcgatt catctggaga
    18001 tttgtaattt agagtccttg tcttgattag aggtgtataa agctttttat tagacatttt
    18061 taaattccat tacaatttgc ttataaacta ggtttttaaa ggtgactatg gtaagataat
    18121 agagatatta ttttgtgatt aggagtgtat atatgaataa aaatggagaa tggagtgctg
    18181 cacgacggat attcattata ttaatggatg ttattgtata caatgcagca atatatagta
    18241 gtttcttact gaagtttcgt ggagagatcc cattacgaaa tttagaaact ttccaacact
    18301 cagcaattat tatttcagtg atttttattg cgttgaacat tttgttgggt gcatatgttt
    18361 tttataatcg aatgatcagt gacatcgtgt ttgtcacagt catcggtcag gtattgatga
    18421 cactagggat catggtagtg acttttgcgg gccgctggtt tgcttttcca cgtcttgtta
    18481 tcttactttc atttgttctc ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata
    18541 aactttattt acgcgtaagt cgagataaaa aggtcgtcat tattgggtta gaaaaggatg
    18601 tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa caaacataaa gtcgaagcag
    18661 tcattcttga taactattat gcaaacatca aaaatatgat taaagacatc gatattgtct
    18721 atcttgcttc tcatatcgaa gaaactgaaa
//
LOCUS       DACCWV010000050        18749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCWV010000050 DACCWV010000000
VERSION     DACCWV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.050"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            54..1739
                     /locus_tag="IVW84_001440"
     CDS             54..1739
                     /locus_tag="IVW84_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7917644.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1753..3036
                     /locus_tag="IVW84_001441"
                     /pseudo
     CDS             1753..3036
                     /locus_tag="IVW84_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            3106..3975
                     /locus_tag="IVW84_001442"
     CDS             3106..3975
                     /locus_tag="IVW84_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917645.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            3972..4808
                     /locus_tag="IVW84_001443"
     CDS             3972..4808
                     /locus_tag="IVW84_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917646.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4834..5901
                     /locus_tag="IVW84_001444"
     CDS             4834..5901
                     /locus_tag="IVW84_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7917647.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5898..6725
                     /locus_tag="IVW84_001445"
     CDS             5898..6725
                     /locus_tag="IVW84_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7917648.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6770..7795
                     /locus_tag="IVW84_001446"
     CDS             6770..7795
                     /locus_tag="IVW84_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7917649.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7792..9975
                     /locus_tag="IVW84_001447"
     CDS             7792..9975
                     /locus_tag="IVW84_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7917650.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            9975..11103
                     /locus_tag="IVW84_001448"
                     /pseudo
     CDS             9975..11103
                     /locus_tag="IVW84_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            11100..11777
                     /gene="pgmB"
                     /locus_tag="IVW84_001449"
     CDS             11100..11777
                     /gene="pgmB"
                     /locus_tag="IVW84_001449"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7917651.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11861..12388
                     /gene="msrA"
                     /locus_tag="IVW84_001450"
     CDS             11861..12388
                     /gene="msrA"
                     /locus_tag="IVW84_001450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7917652.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12589..13275)
                     /locus_tag="IVW84_001451"
     CDS             complement(12589..13275)
                     /locus_tag="IVW84_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7917653.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13355..14893
                     /locus_tag="IVW84_001452"
     CDS             13355..14893
                     /locus_tag="IVW84_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7917654.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15013..15480
                     /locus_tag="IVW84_001453"
     CDS             15013..15480
                     /locus_tag="IVW84_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7917655.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            15999..16442
                     /gene="rplM"
                     /locus_tag="IVW84_001454"
     CDS             15999..16442
                     /gene="rplM"
                     /locus_tag="IVW84_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7917656.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16458..16850
                     /gene="rpsI"
                     /locus_tag="IVW84_001455"
     CDS             16458..16850
                     /gene="rpsI"
                     /locus_tag="IVW84_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7917657.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16939..18165)
                     /locus_tag="IVW84_001456"
     CDS             complement(16939..18165)
                     /locus_tag="IVW84_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7917658.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(18237..18395)
                     /locus_tag="IVW84_001457"
     CDS             complement(18237..18395)
                     /locus_tag="IVW84_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7917659.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
BASE COUNT         6068 a         3429 c         3966 g         5286 t
ORIGIN      
        1 ccataatatg atcaaatcgt tgggaacgct cccaaaaaag aaaggggaat ccgatgtata
       61 caaaactttt acaagcgatt tatcgcgact cagatttgtc tgagaaaacc gcctctatga
      121 ttgaacaaaa agtcagtgtt tatcagcaga agaaaggcca ggcaggcaaa ctgcattggg
      181 acgaagaaga tattttcttg attacttatg gggatcaatt ttttgaagag cagcaaccaa
      241 ctttgcgtac atttaagaaa ttttatcaaa agtatttggc agatacattt agagttgtac
      301 atttcttgcc atttttccca tatacatctg atgatggttt ctccgttgta aattatcagg
      361 caatcaacga ccgtttgggc acatgggaag atatagaaga aatgagaagc gaaacacggt
      421 taatgttcga ctttgtctgc aatcatatgt ctgccgaaag tacttggttt caggaatatt
      481 taaacttgaa tgatacgtac aaagattttt ttattgattt gccactggat acagatacaa
      541 gcagcgtgac tagaccaagg accagcccgt tacttacaac gttcattgac aaaaacggga
      601 aagaaaaaca cgtatggacg acttttagcg aagatcaagt agacttgaat tttaaaaatc
      661 cagaagtttt attgaaaatg atcgatattc ttttgtttta cttagaaaaa ggcgcttcat
      721 ttattcgctt ggacgctgtg ggatttttat ggaaagaact aggaacttct tgtatacacc
      781 tgccagaaac gcataaaatc atccaattat tccgtgcaat cgctgatgaa gcagctccag
      841 gaacggtatt gatcactgag acaaatgtgc cacacaaaga taatatcagt tattttggga
      901 atggcgagaa tgaagcacag atggtctatc agttttcgct accacctttg acccttcaag
      961 cgattcgttc gggtaataca agtaagttga aagaatggtt aaaaaccatc gatttcgcat
     1021 cgaaaaaaac aacttttttt aattttttag cctcccatga cggtatcgga ttgaatccgg
     1081 tacgaggtat tctgccggaa aaaacgatcc ttgaattagt ggaacagctg aagaaagagg
     1141 gtgcccgagt aaactacaag aaaaacccag atggaacgac cagtccatat gagataaacg
     1201 ctacgtatct agatgcgtta agttctccaa cagatacgac ggaatggaga agcaaacgct
     1261 ttttagctgc tcattcattt ttggtaacgt taccaggcgt gccggcaatt tatattcaaa
     1321 gtgtgctggg aagtcgaaat gattacaaag gcgtagaaaa aacaggagag aatcgaaaaa
     1381 tcaatcggca aaaatttttg ttagcagata ttgagaaaga gttgcagaca gatccattaa
     1441 gaacagcagt atatggtgaa ttgacccaga tgctatctat tcgaaaaaaa gaaaaagcct
     1501 tccatccgaa tagtttgatt tacttactgg actcagcaga ttcttttatc agttttattc
     1561 gcgaagcgaa ggaagagaaa gtcttagtta tccataattt ttccaatcaa tcagcaggtt
     1621 atcttttacc aaaaggagat tacctagacc tgttttctgg acaaattatc cattgcagtc
     1681 aagaacgaaa gttgatattc cagccgtatg aatatatgtg gctgaaaata ataaaataag
     1741 aggtggaaaa aaatgaaaaa aattattgtt actacctgtc tgctagctac tggatttctt
     1801 cttactgctt gtggtgaaag tcaaagctct aacgaatcac aacagacaac gattcaattc
     1861 atgcactctt ctgtcgaaca ggaacgatta agtgtgatca atgaactggt tgctgatttt
     1921 gaaaaagaaa atccagacat caaaatcaag caagtgccgg tagaagaatc agcgttcaat
     1981 acaaaaatcg ttacgttagc tcgttccggt aaattaccag aagtaatgga agtcagtcag
     2041 gattttgcta aagtgatgga taaagatgaa ctgattgatt agacggcagt ccaaaacgtt
     2101 atcgaggaag ctggagaaga taattattat gacggagcca aaaaccttgt tcgttcagaa
     2161 gatggaaaaa gctatattgc tgcaccaatt agcggctggg ttcaaggtat ttggtatgac
     2221 aaagaaaaat tatcagaagc tggtttttca gaaccagaaa attgggatga tattttaaaa
     2281 attgctcaac atttcacaga taaagagaat aaacaatatg gaattgctat gcctactgct
     2341 gaaggaacaa tgagtgagca agcgttctct caatttgctt tgtcaaatgg agcaaatgtt
     2401 ttggatgatg aaggcgaggt aacgatcgat acggaaaaga tgagagaagc attgtcgttt
     2461 tatcaaaact tatcccaata cacgatgcca ggctctaatg atgtgacaga aatcaaagat
     2521 gctttcatga atggaacagc accaatggca atctattcaa cttacatttt gccttctgtt
     2581 tatgaagcgg ggaactcaga aaatatcggt tttgctatcc caacacaaaa agatcaagct
     2641 gtctatggca cagtatctgg attgacgatt tcttctggtc tagacgagaa acaaaaagag
     2701 gctgctgaag cattcacagc tttcttatct caaccgaaaa acatggaaaa gtgggtcttg
     2761 atgtctcctg gtggtgctca accagtgaat aaacaagtag tcgaactgga tggttacaaa
     2821 gaaaatgaag tcatcaagtc ttttggcgag ctgccatctg agattgctag tgcttttgat
     2881 gaagtacaag tctttggact agtcggggaa aagaatttca cgaaaatggg agatatcaca
     2941 agctcaggtg tgatcggaaa agcagtgaac caagtaactg tcggaaacga agatgtcgat
     3001 caagctttag ctgacgcaca aaaaaatacg aaataacaag aagttctgcc aatggtgttc
     3061 gaaaaaagta gatagctatt ggcagattat cagggggagg tttcaatgaa tcgtccaagg
     3121 aaacattctg acttaaaatt agccatggga cttttagcac caagcatgct tttattagct
     3181 gttttagtgc tgattccaat gctttccaat attcagatca gttttttcga tatgccgctg
     3241 aatcctaact cagacgccgt ttttatcgga ttaagcaatt atcttgaaat tttaagtgat
     3301 agtgaatttt atcgttcttt aggtttaaca gttgcttaca cattcttagt tgtagcaggc
     3361 agtacagctt taggattagc cgtagctatt ttgcttaacc gcaagttcag atttaggaaa
     3421 acggtgcgtt cactgatttt attaagttat gttacgccat ctatttcttt gatttttgct
     3481 tggaaatata tgttcaataa tagttatgga atcgtcaatg atctgttagt agataggttg
     3541 cagcttttca acgaagcacc gctgtggttc gaccagccag ttgctagctt ctttatggtc
     3601 gttttgtttg ctatctggag atactatccg tatgcgttta tttcattttt ggctattttg
     3661 caaacagtcg acaaaacgct ttatgaagca gctgaaatgg acggtgcaaa tgcttggcag
     3721 aagttcaaga tcgtgacact gccggcaatc aaaccagtac tagcaactgt gattgtttta
     3781 cgatctatct ggatgttcta tatgtttaca gatgtctatc tactgacgaa taaagtcaat
     3841 atcttaggcg tttatttgta tcagacagcc tttgcgttca atgatcttgg aaaagcagca
     3901 gctatatcga ttctattgtt catcattatt gcagcggtcg tcttgctctc acgaaagaag
     3961 gtggacttga aatgaaaaat aaaaagagca ggaaaatctt attttacgcg acgattttgt
     4021 tgctattagc tgtcaatttg tttccatttt tgatcatgct gatgacatca ttcaaggatt
     4081 cacaagaagc aattgcaacg acacaaacta tttttccgaa agaatggact tttgaacatt
     4141 atcaggatat tttcaatgct gagatttttc cattcatcat gtatttcaaa aatagtttag
     4201 tgatctcctt gatagcctcc gtaactgcag tagtgattgg gatcagcggg gcatacgctt
     4261 tgtcaaaact acgcttcaaa gctcgctcga aaatcaatgc tagcttttac accgtttata
     4321 tgttttccgg tattttgatc gttgttccat tatttaaaat catttccggt ctaggtttgt
     4381 atgataccaa tacagcactg attatcacga tggtcgttca aacacttcct actgcgatat
     4441 tcatgctaaa aagttatttt gacacgattc cgactgatat tgaagaagca gcaatgatcg
     4501 atggtcttag ccggatccag atcattattc ggatcattat accattggct atttctggaa
     4561 tcatttctgt cttcgtttat tgctttatgg tttcgtggaa tgattattta tttgcttcga
     4621 ttttcctgtc gacctccgaa aaatttactt taccaatcgg tttgaacaca ttattcagta
     4681 caccagacta catttgggga cgaatgatgg ccgcttcatt ggtgaccgct ttaccagtca
     4741 tcattatgta tgcgttatct gaacgattta ttaaaggtga tttagtcgaa ggcggcgtaa
     4801 aaggataaaa agaaaagaaa gaggaaagaa aagatgaaaa aattagttgc attagaacca
     4861 agaatagccg gattcaaaga ttacgaagac cgtgagatcg aaaagaacga agtacgtatc
     4921 cgagtaattt tcgctactcc aaaaccagga acagaaattg tcgactttag aggattaagt
     4981 ccatttatcg atgaagaatt ttcagaagaa tggcgcctat tcttgccaag agaagaagga
     5041 agccaacgcg gtgtggtatt tggcgaattt caattaggaa atatgattgt cggtacgatc
     5101 gaagaaacgg gcagtttggt gacagagtat caaaaaggag agttagtctg cacttatgga
     5161 cccattcgtg aaacgctgat tacaaacggt gttgataatt ataaactacg taaacttgat
     5221 cggttggatc aatggaaaaa tgcagtatgt tacgatcctg ctcaattcgc tttaggtggt
     5281 atccgggaag cacatgttcg agcaggtgat gctgtagcaa tcttcggttt aggcgctatc
     5341 gggcaaattg cgattcaatt agctaaaaaa gcaggtgcta ctaccatcgt tgggatcgat
     5401 cccatagcgc agcgtagaga attagcagaa aaatacggtg ccgattacac gctagatcct
     5461 atgaaagcag atgtcggttt tcgtctaaag gaattgacag ataaacgtgg agtagattca
     5521 gttattgaaa cgagtggcag tgctgccgca ttacaagctt ctttaagagg gatcggctat
     5581 ggcgggatca tttcttatgt tgcctttgct aaaccttttc cagctgggtt taatcttggt
     5641 cgcgaagcac atttcaataa tgcaaaaatc gtctttgctc gagcatctag tgaaccaaac
     5701 ccagattacc caagatggaa ccgtaaacga atcgaggaca catgttggga attattgatg
     5761 aacggttact tggattgtga agaaatcatt gatccagttg tgccatttgc agaaagcgca
     5821 gaagcttaca tgcgttatgt agaccagcac ccagaagaaa gtatcaaaat gggcgttcaa
     5881 gtccgaggag gagaacaatg aaattcggta cgcaagatca agcatttttc ccaagagaca
     5941 ttttagaaaa atttagattt atcaaagact gtggatttga agaatacgaa atcgacggta
     6001 agttgttaat agaaaatatc gacacagtca aacaggcaat caaagaaaca gggatcccag
     6061 tagtgacagc ttgtaacggc tacgatgggt ggatcggtga ctttatcgaa gaacggcgtc
     6121 ttaatggtgt acgtcaaatt gctgagattt tgcgtgcact aagcgaggtt ggaggcaaag
     6181 gaatcgttat cccagcagct tggggaatgt tcacttatcg attaccgccg atggtttcac
     6241 cccgaagcaa agaaggagac tataaagcag tcagcgaatc acttcgttat ttagataaag
     6301 tagccgaaga aacaggtacg gttatttatt tggagccttt gaaccgatac caagaccata
     6361 tgatcaattt gttagcggat gcgcgtcatt acattgactc taatcaattg aaacatgtgc
     6421 agatcatcgc tgatttttat catatgaata ttgaagaaga tgatatttca aaagcattga
     6481 ctacgcaaaa agagtatgtg ggtcatatcc atttagcaga taatcatcgc taccaaccag
     6541 gatctggcgc aattgatttt actcgccatt ttcaaacgct acttcaaaat gagtatgaag
     6601 gttatatggt atatgaatgt cgtttgcgag gagaaaatga agtgaaaatt tatcaggaat
     6661 cattgcaata cttgaaaaac tgtctggaaa cagcgcgtca tttaaatgat ttagagaaag
     6721 aatagagaaa gaataacaat aagtaaataa gcaaaggaga gagaaataaa tgaagaaagt
     6781 agcaatcata ggcgctggac aagtagcaga aaaagtacat gctgcttatt atcaaaaaag
     6841 atcagatgtc ttagaattgg ccgctgtggt tgatccagtc agccagcgag cagaagactt
     6901 ttgccgacgt acaggatttt ctcatgcgta tacatcagtt acacaaatgc tgaatgagat
     6961 ttctcccgat atcgtgagtg tctgtacagc caatcgcttt cacttcgaac atgtattggc
     7021 tgccctcgaa gctaatgctt cagtgatgtg cgagaaacca ccagctatga cagctgatca
     7081 agcaagaaag atggcagaga cagccgataa aaacgggcag attttggctt atggattcca
     7141 gcatcgtttt tctgacgaag caacaatgat caagaatcat cttgatgttc tcggggatat
     7201 ttattacgta gaagcgaatg cgcttcgtcg gtctggtgtt ccaggctggg gaacgtttat
     7261 cgacaaagaa cttcaaggag gcgggccttt gatcgattat ggcattcaca tgcttgatac
     7321 agcgatgtat attactggat ttcctaaagt aaaaagcgtt cgggcgtaca gcttccaaaa
     7381 aattggtacg aaaaaaaatt cagggaaatt cgggaaatgg gatccaagca aattcacagt
     7441 agaagatgca atttttggca cactggaatt agaaaacggc aagatcatcc aactgaatac
     7501 ttcgtttgct ttgaatatca gagaagacaa actgaatgtg gctttttgtg gcgatcaggc
     7561 aggtgctaat ctgtatccag gcgaactatt cgaagatgta gatggaaagc tggtttcctt
     7621 acaaaagacc acgccagtta atgacccaca tgaactaagt atggctcatt ttgtgaatcg
     7681 ggcgctcggt gaccagactc agatgattgc agatggctgg caaggatatg tcatccagaa
     7741 tctggtggaa aatttatatc gttcggcaga aacaggagaa gttattacct tatgaaagta
     7801 gaagaaaaac agtttgattt agagtatatc aataagtatg cgagtcagat gaccattgga
     7861 aatggatttt ttggtatcag aggaagccag gaggaagact atcggaatca agtaagggga
     7921 atgtttgcag ctggtgtata taaccgacct ttaggagcag aatcagccga gttagtgaat
     7981 ctcccagatg tcatcagatg ggaaataaaa atcgacggtg aacatttttc tcttgagacg
     8041 atgaaagttc ttgcatacga gcgggcacta gatttagaaa tgggagagct taaacgctat
     8101 gcgctcattg aaactgctca aggaaaaaaa gtggaagtca ttacttgtcg tgttgctgat
     8161 caaaataatc tccatcaggc agccctgaaa gtggtcatca agccgttgac cgaagactgc
     8221 caagtagatg tgaaaacggg tatcgatgga cagcaaacca attttggtac acagcagttg
     8281 gttgaaaaag aactgcgagt atttggtgac gatttgatca gtgcatccta ccagacaaca
     8341 gaaagtaaaa ttgatattgg gattgcagct aaattcaata aagcaggtgt attccaagca
     8401 aaaaatcgcc ggatcaccgc ttccttttca gaaaagatca gaaaaaatac gtcctttacg
     8461 ctggagaaga tcatcgtgct ctctacctct cttcagcaaa cagacccgtt taagaaaagt
     8521 attgcccaag cccagaaaag cgttagttat acagaaattt tagagcagtc gatgtcttat
     8581 tggacagatt tttggcatca gcatcggata acgatccaag gcgattcttc tttcgatcag
     8641 ttagcgatgg acttcgctgc ttaccattta cagattatga ctccccgcaa tgatccaagt
     8701 gttagtgtag gggcgaaggg actaactggc gaaggttata aagggcatgt tttctgggat
     8761 acggaaattt ttatcctgcc tttcttttta tatcagtttc ctgaagtagc aaagaatttg
     8821 ctgctgtacc gttatgatcg attagaaggc gcacggcaaa aagcaagaga ttatcactat
     8881 caaggtgcac ttttcccatg ggaaagcgca cacactggtc aggaagagac acccaaattt
     8941 gctgcattga atatcaaaac cggaacaaga caagaagttg cttcagctgc cgcagaacat
     9001 catatcagtg cagaccttgc ttatgccgtt caagagtatg tccaagcaac agaggatcaa
     9061 tcgtttttag aaacaaaggg aaaaacgctc attcatgaaa cagcagcttt ttggctgagc
     9121 agagctgtaa aagtcgatga tcatttagag atacacagag tcattggtcc agatgaatac
     9181 acagaatata tcgacaataa tgcatatacg aactatctgg cgcattataa tgtggcctta
     9241 gctgttaaat atcaagctgg cgatccgctt tttttgaaag aatgtcaaaa ttttttagat
     9301 aagttatatt tgccagtgcc taacaaagaa cagctgattc cgcaagatga cacgtatctc
     9361 agtaagccag ttattgattt gaccacttac aaagaacgtc ctggcagcca gtctatttta
     9421 cttgattatg cccgtagcga agtaaatgaa ctccaagtca gcaagcaagc agatcttgtc
     9481 atgctgttat acttattacc tgagtatttt tctcaaccaa tcgtttctaa gaatttgcat
     9541 tactatgaaa acaaaacaat ccacgactca tcgttgagta aagcgattca cgccatagag
     9601 gcttgccgta tccaagaaat ggaatgggcg tatcatttat tccaagaagc gtgcctgatt
     9661 gatttaggac cagatccgca ttcatcagat gacggaattc atgctgcttc attaggagca
     9721 ctatggctag cagttatatt tggtttttca ggtattacag taacgaatgg aactctttct
     9781 atttctccaa gacttccttc agcatgggaa aaaatttctt ttccttttat ttggaaaaat
     9841 cacaggttgc tatttgtttt gacaaaaaaa caaatagtaa taaaaaaaga gacgaaagaa
     9901 acattaccta tttttgtcaa tggagaccaa taccagctgg aaaaagaact gatcttggaa
     9961 cggaggggaa aataatgcgt attctgattg cgccagattc tttcaaagaa agtctgacag
    10021 ccttagaggc agcacaagcc attcaatcag gttttcaaga agtttttgtt caggctgaat
    10081 atgatctgct acctataggc gatggaggag aaggaacggt ttcatcatta gcagacagtt
    10141 taaggttgaa gcggcaaaca gcagtcgtta ctaatgcatt tggcaaaaac aaagaaatcc
    10201 agtacgcttc agacggtcaa actgcgattt ttgaaatggc agaaatcgtc ggattgaaag
    10261 atgtcccact aaaccaacaa cagccactta ggttatccac gcgaggagta ggagaactga
    10321 tacgacaatt gttgcaacaa ggtgttcgac acatcatcat cggcgttggc ggaagtagta
    10381 ccaacgatgg cggcatcggc atggcagaag gtctaggtta tgctttttac gatcaagata
    10441 atcaaaaagt agaagcactg ggagaaaatc taggtaaaat tgctacagtt gataaaacga
    10501 acgtgcctat tgagctgatt aaagccaaaa tcactgtcat tactgatgtt actaatcttt
    10561 tatgcggaaa agaaggcgct tcttttgttt tcggaccaca aaaaggcttg aaagaaaatg
    10621 aattagcagt ggctgaccaa gccatgcatg atttttatat gagatttgcc cctcaagtaa
    10681 tcgaggcatc gggcagtagt gctggtggtg gaatgggtgc aggactgtta gcatttgctt
    10741 cagggaaaat agtctctggc atcgactacg tattggattt actggagttc gatacgggta
    10801 cagcaagcag acctcgtaat cgtaggcgaa ggacgtctag atggacaaag tctaaaagga
    10861 aaagcgccag ttgggattgc tagaagagta ccagagggcg ttcctgtgat agctatttgc
    10921 ggaagtgttg gtaaaggaag cgaaaaagca gcagatgttg gcatttcggc cgtttttccg
    10981 atccttcctg cagctgtgcc tttacaagaa gctttacagc aagccactga taatcttcat
    11041 cgcacagcta aaaacatcgc atctttactt caagtcgttc attttcaagg aggtaatcga
    11101 tgaaaatcaa agcagcacta ttcgatttag atggcgttct tgtagacaca gctcgttatc
    11161 attacgaagc gtggctggtt ttagctaacc agctgtccat tccattcact gagaaagaaa
    11221 acgagcaact gaaaggaata tccagaaccg agtcactaga gcgattgctg tcatttggaa
    11281 aaatggaaca gaaattctca gaaaaagaga aatcagcctt tgctgagcaa aaaaacaatc
    11341 tatatcttca agcgattcaa aaaatggatg aaacatccgt tttgcctggt gcaatagctg
    11401 ttcttgagta cttgaaaaaa acaaacatca agatcggatt gggatcagca agcaaaaatg
    11461 cacgcctcat tttagaaaaa acgaatttga caagttattt tgatgtcctt attgacggca
    11521 cgcaagtatc aaaagcaaaa cctgatccag aagtttttct caaaggagca cagcaattga
    11581 atgtaccgcc aaatgcatgc ttggtcattg aagattcaga agctggttgt caagcagctt
    11641 tggctggtaa catgcatgta ttgggaatag gtgaaaacat aaatcttcct tctgctgaat
    11701 atgtgattcc tgatttaacc gtttttgatc aagtacgttc gttttggcat ttatcagaag
    11761 ccgtatcacg tatataatag tttccgtttt ttctgtcaca agcaaaacgt tatcctcctt
    11821 ttggaaatca aggtatgatg agaaaaaagg gggattttat atgacaaaaa aagcagtttt
    11881 cgcaggcgga tgcttttggt gtatggtcaa accatttgaa gagcagccag ggatcatttc
    11941 tgtgacatcc ggttatactg gcgggcatgt accaaatcca acttatgaac aagtaacaac
    12001 aggcacaaca gggcatacag aagcagtaga gatcgaatat gatccagaag ttatttctta
    12061 tgatcagttg gtggaaatct attggcagca aaccgatccg actgacgcat taggacagtt
    12121 cgctgatcgc ggtgattctt atcgtccagt gatctactat aatgatgaag aagaacgaaa
    12181 tattgcagag cgttcaaaac aagcattgca agacagcggg cgattcgata agccaatcgt
    12241 gacaaaaatc gaaccaagaa gcgaatttta tcctgcagaa gattaccatc aggattatta
    12301 caagaaaaac aagctccatt acaatcttta tcgagaaggt tctggacgtg caggtttcct
    12361 tcgtcgacat tggggcgtca agaaataaga agccgaaaaa gctacttatg gaacaccgta
    12421 tatcagaggt tgtaaaacca gcattttgag ctaatactga gggtcagctg attgaacacc
    12481 atttagtgga gaggttgtga acctgtcgcc tagctccaag tcacaaggat ttggtaagag
    12541 aacactatat atcagaggtt gtgacaagtc gtttgtcaca accttttttc aatacctaag
    12601 atgaaggaca atcagcaacg cactgctcaa aaaaggaatg aaaggcagct tttccaactt
    12661 ttttttatga cagataaagt aaacagaaaa aacggatatt ccaaacagac tagcaagaaa
    12721 caagatctga agcaactctt ctagagacaa gccaccactc caagaaagca tcaacagagt
    12781 atcgccagct ccgagatagg attgaccata gcgaaggcac acaccgccta cgataaacgt
    12841 taacagcaca ctttgccatt ggaagacgcc ggcctgcaac caaaaaatcc ataaaattgc
    12901 ccaagaacag aatagcgtgt gagaatggac catgaagtaa aataaatcca tcaacgaaaa
    12961 tagcaaagcc gaaagcatcc atatgaacgt cgaaaaattt cttgcggaag aaaaagtaaa
    13021 gaaccaggca aaaagaccac cacataaggc ttctgccaac aaatagtaga agggcaaccg
    13081 acacctgcaa tagcggcagc gaaatcgttg gacgatgatc gagagaatcg gaatcaattc
    13141 ataccaagaa agcgaacgac agcatttgac acaatgagat ctaggataaa taatagagtg
    13201 acctaacgga atccgctggg cggttaaaca taaaaatgat ccgatacagc aaccaatgat
    13261 aaaataaagt aacacgccat ctcccccttt tcaaaaccaa gatacgcaag cttaaactaa
    13321 atttttgtac atacataaaa tttgaggtga aaaaatgaac gtttcaatcg gattagatat
    13381 cggaaccaca caaaccaaag cagttgcttt cacagatgaa gcccaagaag cagcatccgc
    13441 ttattttcgc taccctttga tccaagaaac acaagggatg gcagaacaag atcaggaatt
    13501 gatctttcaa ggtgtctgca ccgttataaa agaagtaatt aaacagttgc cttcagacac
    13561 aatcattcaa tttgtctcct tctcctctgc gatgcatggc ctgatattgt tggatgaaga
    13621 gaaaacaccg ttatctcgaa tgatcacttg ggcagataac cgagcagcgg acgaagcaga
    13681 aaaactgaaa tataccgtgc ttggcaaaga attttatcgg cagactggga cgccgattca
    13741 tccaatgaca ccattgaaca aaatcaagtg gctgcaaaac agccaacctg aattattcaa
    13801 aaaaactgct tattttgtcg gtatcaaaga ctatatcttt tatagattgt ttggcgaact
    13861 gatcagcgac tacggaacag cttcaggaac tggatatttt gatatccatg aattccaatg
    13921 ggcagaagaa gtgcttgact acttgaacat tcgaatagag caattgccgc aagtctttcc
    13981 ttccacctat caagtaacag gtctaccatc aaaaacagca gcagaactag gtttgccaaa
    14041 tgatatatca tttgttttgg gaggggcaga cgggccgctt tctaatctag ggttaggtgc
    14101 ttttggtgat acgatcgccg ctttgaccgt aggaacgagc ggagcacttc gttttattac
    14161 tactcagcct cagttccatc cagaaatgga gacgttttgt tatgttctgg atcagaaaca
    14221 ttgggtagtg ggaggagcga ctagcaatgg tgctggcatc tttgattggg cagggcatac
    14281 atttttacag gaagtcgttc aatctgctaa agaaaaagga gaaaatccat atgatgcact
    14341 gctttcggct atcgcttttg tgccagcggg tgccaatggt ctgatttttc atccctattt
    14401 acttggtgag cgagcaccgc tttgggacgc agatgcgttt ggcagtttta tcggtttaca
    14461 gcggcaacat acagaaaaag aaatgatgaa ggcgattcta gaagggatct gtctcaacct
    14521 ttatcggatt ttaaaaggag tttgccagga aaatcagctc acagagatcc gtgctacagg
    14581 cgggtttgct caatctccga tttttcgtca gatgatggct gatgtactgg gttacccgct
    14641 tgttttccct actgtcacag aagcatccgc attaggagct gtgattttag gctggagaag
    14701 catcggaaag atccaaagta tcagtgaagc acaggaattg atagaattga aagagcaagt
    14761 tggagtacaa gaaactgatc ataaaaaata tcagaaaatc tatccattat ttgtttcgac
    14821 acaaaagcat ttagcacaga cttatcagca atttgctcaa ctgcgagaag aattagctga
    14881 ttctgaaaaa tgattggctt tacggataaa aaagcgaact gaaaatcgtt ctcaggtaaa
    14941 tgattttgtt gtacttttca ttgaaagtct actattattt taagtgtaag aaaatgttag
    15001 gaggcaagaa agatgaaatt ccaacaaacc aaagaagtat taaatcaatt agttgcagat
    15061 ctaagccaaa tgtctgttgt tgttcaccaa acacattggt acatgagagg accaggtttc
    15121 cttacattgc acccaatgat ggatgagttc atggatgatt tgaacgctca gttagatgaa
    15181 gtatccgaac gcttgatcac attagacggt tcaccttatt ctacattgcg tgaatttgca
    15241 gataacacaa aaatccctga tgaaattggc cgctgggatc gtactattcc agaacgtttg
    15301 gaaacacttg ttgcaggcta tcgttatcta gcagatcttt accaaaaagg aatcgaagtt
    15361 tctggagaag aaggcgacga tccaacacaa gatatcttta tcgaattcaa aacagccaca
    15421 gaaaaacgta tctggatgat ccaagctcat ttgggcaaag cgccagagat cgacgcgtaa
    15481 aaactgttgt tgaacttgac cgaaaaagag gttgtgaaat cagcgttttg acctgagtat
    15541 tagagaaaca aacgtcaaaa tagttcctca tattttgccg ttttttgagc taataccgag
    15601 ggtcagctga ttgaacaccg tttaccaaga ggttgtaagc ctgccgttca gctccgagtc
    15661 acaagaaaca attttaaaaa acagcttctt atgttttatg gatattgtga cttgtgaccg
    15721 aggagttggc aggagaacac cgtttattca aaataagggg ttgtgacaag acttttgtca
    15781 cagcctcttt ttgtttttca ttttgacagg aaacgaccct tttatataaa agaaagaaaa
    15841 gaagtaaagt ttcatataaa tttttcaatc aagcagaaaa acgttgacat tgactagatt
    15901 agcgggtaat atgttaaatg gtattgtttg ccccacaaag agccccggaa actcgaagtg
    15961 tatgtcaaac atcagaactt aaattggagg aaaataacgt gcgtacaaca tatatggcca
    16021 aaccaggcga agtagaacgt aaatggtatg tagtagacgc aactgatgtt ccattgggac
    16081 gtctttcaac agttgttgca tctgtactac gtggtaaaaa taaaccaact ttcacacctc
    16141 atttggatac tggcgatttc gtcatcgtca tcaatgcaga caaagtgaaa ttaacaggta
    16201 aaaaagcgac tgacaaaatt tattaccgtc acagcaacta cccaggagga ttgaaatcaa
    16261 tcactgctgg cgaattgcgt gctaaaaatt ctcgtcgttt gatcgaaact tctgtaaaag
    16321 gtatgcttcc taaaaacact ttaggccgca aacaatttac aaaattgaac gtatacggtg
    16381 gagcagaaca tccacatgca gcacaacaac cagaagtttt agacatcaca aacttaatct
    16441 aataggaggg aatttcattg gcacaagttc aatatatcgg cacaggccgt cgtaaaaacg
    16501 cagttgctcg cgtacgtcta gtaccaggaa ctggcaaaat tactgtaaat aaaaaagatg
    16561 ttgaagaata cattccacat gctgacttgc gtgaagtaat caatcaacct ttcgctgtta
    16621 ctgaaacaaa aggcgcatat gacgtatttg ttaacgtaaa cggcggagga tacgctggac
    16681 aatcaggagc tatccgtcat ggtatcgctc gtgcattgct agaagttgat cctgacttcc
    16741 gtttagctct taaacgtgct ggcttactta ctcgtgacgc acgtatggtc gaacgtaaaa
    16801 aaccaggtct taaaaaagct cgtaaagctt cacaattctc aaaacgttaa tattcttttt
    16861 atatcaatat ttcaggacac ttttcaatta tttggaaagt gtcttttttt ataataagaa
    16921 cacatttgag aacacatgtt aatcacactt attcaatcct ttttgaaatg cttcaaaaac
    16981 agatttatca actacgtcag gtgtgttcac gtatattttt gtagtagaaa tatccgagtg
    17041 cgttaaagca ttagagattt gattcatatt tgctccacct tccctagcta tagttgcata
    17101 ggtatgacga agtttgtgtg gagataattg agccaattca ggatgccttt tttttattgc
    17161 attgattcga tagtttaagt agtcagcgtg aacaggacaa tttacttctc cagaaggttt
    17221 ggaataggta aagaggtact ggtcaggtgt ttgtataatt tttagtttca acaattgttc
    17281 agcctgagct gatttccatt ctcttaataa tgtcatcaca acttctggtg cctgaataac
    17341 ggtgtctttc caaccttttg tatgtgtctt tctttgatat ttatctaaag catatttgag
    17401 acgaaccgta tttttattaa aatcaatata tttccattgc agagcataag tctcactttt
    17461 tctatcacct agatataagg taagcatgaa aagtgtgtaa tcgtgaagtg taagcaagtg
    17521 attatttaag tcattgttta ctgcttctat ccaatcagtc agctcttctg atgataatgc
    17581 ttgattccct tcccttatcc ttttttcttc taaagccatt ttcttaggcg cggtaataca
    17641 ttgaataatt ttggtagttc ggttgaattc gatataacat agtatttcgg caatatcaaa
    17701 catagagcga acatagcctt tgataatttt tatatttgcg tattctttcg atttctttgt
    17761 taattcatct gatattatct cagtatttga attcagataa ttcattgcat attcaccaaa
    17821 cattggaagg atgtgcaatc ggaatatatc cttagtatta ctaattgtta cattacttgg
    17881 agccctgttt gacctagtag tttgacctag ttcatatcta ggaagccatt ttgattcata
    17941 aaaatcttta aattttattt ttcctttcaa ttctagtgag tttgcattct cctctcttaa
    18001 aactgtttca atcttctttt tcatttccct ttcggcctta atagctaatt gcctagttgg
    18061 aaatactttt tgatacttct ctgatgcaat tccagtgatt tctctgaatt tttttggata
    18121 cctcagatta gttttatatt tcccatttga aagctttgta ggcaaacttc ctcactcctt
    18181 taaaaagtaa ggcagtacat ttcgctattt atagtatatg tgacgagaag aaaatttcaa
    18241 ttttttttga atgtattcat gaaatctttg atatcctcta cgtcaaagcg aactacgcca
    18301 tcaattttta ttactggaaa attatataca cgaatccact tatctaaagt attattatca
    18361 attcctagaa attcacaggc ttgtttttta ttcaagtagg ctttcgattt tataaaatca
    18421 tctcctaaaa taatagttta aaatttttga accgctttgg gtaagcacat aggaatttca
    18481 cctctcccca gtttacggcg agccttacgg aagtatcatt atttactgct attgtcatgg
    18541 cattttgttg tcccagaaca aaagaaatct ctaagtagtt gtcgctaact aggtcatggc
    18601 gtacttgtat ttctagctcg aatagcaggg aacgttattc aaaaattata tctaattatc
    18661 aaagatcaat ataggaatat catcctaatc actattaata acaagaagaa gattaaaaaa
    18721 gaaacaaaaa cggaaaaaaa tttatcttg
//
LOCUS       DACCWV010000051        17589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCWV010000051 DACCWV010000000
VERSION     DACCWV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.051"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            363..731
                     /locus_tag="IVW84_001458"
     CDS             363..731
                     /locus_tag="IVW84_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7917660.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IVW84_001459"
     CDS             911..1852
                     /locus_tag="IVW84_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7917661.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IVW84_001460"
     CDS             1921..2652
                     /locus_tag="IVW84_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7917662.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IVW84_001461"
     CDS             2796..4976
                     /locus_tag="IVW84_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7917663.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IVW84_001462"
     CDS             4996..5478
                     /locus_tag="IVW84_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7917664.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IVW84_001463"
     CDS             5494..6255
                     /locus_tag="IVW84_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7917665.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IVW84_001464"
     CDS             6386..7720
                     /locus_tag="IVW84_001464"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7917666.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IVW84_001465"
     CDS             8027..8512
                     /locus_tag="IVW84_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7917667.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IVW84_001466"
     CDS             8505..9188
                     /locus_tag="IVW84_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7917668.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IVW84_001467"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IVW84_001467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7917669.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IVW84_001468"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IVW84_001468"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7917670.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNWQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IVW84_001469"
     CDS             12804..13433
                     /locus_tag="IVW84_001469"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7917671.1"
                     /translation="MSELAKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IVW84_001470"
     CDS             13485..13817
                     /locus_tag="IVW84_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7917672.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IVW84_001471"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IVW84_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7917673.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IVW84_001472"
     CDS             14643..16016
                     /locus_tag="IVW84_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7917674.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IVW84_001473"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IVW84_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7917675.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IVW84_001474"
     CDS             17071..17511
                     /locus_tag="IVW84_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7917676.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5993 a         3066 c         3867 g         4663 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagata aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattggcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat ttccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaat
//
LOCUS       DACCWV010000052        17458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCWV010000052 DACCWV010000000
VERSION     DACCWV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.052"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..725)
                     /locus_tag="IVW84_001475"
     CDS             complement(27..725)
                     /locus_tag="IVW84_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7917677.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IVW84_001476"
     CDS             complement(718..2070)
                     /locus_tag="IVW84_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7917678.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IVW84_001477"
     CDS             complement(2293..3693)
                     /locus_tag="IVW84_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7917679.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IVW84_001478"
     CDS             3854..4591
                     /locus_tag="IVW84_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7917680.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IVW84_001479"
     CDS             4716..5195
                     /locus_tag="IVW84_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7917681.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IVW84_001480"
     CDS             5192..5959
                     /locus_tag="IVW84_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917682.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IVW84_001481"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IVW84_001481"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7917683.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IVW84_001482"
     CDS             complement(6862..7464)
                     /locus_tag="IVW84_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7917684.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IVW84_001483"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IVW84_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IVW84_001484"
     CDS             8145..8729
                     /locus_tag="IVW84_001484"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7917685.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IVW84_001485"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IVW84_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7917686.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IVW84_001486"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IVW84_001486"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7917687.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IVW84_001487"
     CDS             10630..10809
                     /locus_tag="IVW84_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917688.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IVW84_001488"
     CDS             10831..11856
                     /locus_tag="IVW84_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7917689.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12047..13291
                     /locus_tag="IVW84_001489"
     CDS             12047..13291
                     /locus_tag="IVW84_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7917690.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13444..14391
                     /locus_tag="IVW84_001490"
     CDS             13444..14391
                     /locus_tag="IVW84_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7917691.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14525..15154
                     /gene="upp"
                     /locus_tag="IVW84_001491"
     CDS             14525..15154
                     /gene="upp"
                     /locus_tag="IVW84_001491"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7917692.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15293..15535)
                     /locus_tag="IVW84_001492"
     CDS             complement(15293..15535)
                     /locus_tag="IVW84_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917693.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15656..15728
                     /locus_tag="IVW84_001493"
     tRNA            15656..15728
                     /locus_tag="IVW84_001493"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15689..15691,aa:Thr,seq:cgt)
     gene            complement(15847..16485)
                     /locus_tag="IVW84_001494"
     CDS             complement(15847..16485)
                     /locus_tag="IVW84_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7917694.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16608..17240)
                     /locus_tag="IVW84_001495"
     CDS             complement(16608..17240)
                     /locus_tag="IVW84_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7917695.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5963 a         3086 c         3454 g         4955 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg tagattacaa
    12061 aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac aagaacataa
    12121 tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg cacaaggaag
    12181 tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg gtgggtgtga
    12241 atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat tcggagcgaa
    12301 atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt atctcgcttt
    12361 ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac atctaacaca
    12421 tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc
    12481 gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac atcaaccaaa
    12541 attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga aattcagaga
    12601 aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg caggattagt
    12661 agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga ctaccactca
    12721 taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag cattagcaaa
    12781 aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc atgtgattgc
    12841 gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat atagtgaaca
    12901 aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg gaaccagagt
    12961 catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt taggtataaa
    13021 tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata aaaactcgat
    13081 tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca cgccagcaat
    13141 cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc
    13201 attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg tacgcgaatt
    13261 aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt aaacagaaaa
    13321 gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga caatatcggt
    13381 gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa
    13441 gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga tgcaaatgcg
    13501 cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact agaagaagta
    13561 caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt ggatactggc
    13621 gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga taaattacca
    13681 gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac ccatgataaa
    13741 ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga atatagtcga
    13801 tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg
    13861 aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc aggagattca
    13921 gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa gggcctccca
    13981 atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac agattcctat
    14041 catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa
    14101 tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag agcaaatata
    14161 aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga tgtattaaga
    14221 gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt tacacaagta
    14281 cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca gacaaaggca
    14341 acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata agctttgctt
    14401 gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc gagtttgact
    14461 agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa ggagagctta
    14521 aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac taactattat
    14581 tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa tcgctatgtt
    14641 gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg aaacaccgat
    14701 cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc caatcttgcg
    14761 cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta aagtgggaca
    14821 tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg tcaaacttcc
    14881 agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa caggcggatc
    14941 agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta aatttgtttg
    15001 tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg atattgacat
    15061 ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc ctggactagg
    15121 ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg gtgtgacaca
    15181 acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag ctttttcaca
    15241 gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa attcaaatct
    15301 tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc aagttcgcat
    15361 tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt gaaatcgttc
    15421 cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg aatttttcac
    15481 caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt ttcataaaac
    15541 ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt aaactaattt
    15601 tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg ataaggccag
    15661 cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt cgatccctat
    15721 agctggcatt aaaagagaag acagatatat caaggatttt taatccattg atacatctgt
    15781 ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta ttatttctat
    15841 aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata tcacatgaac
    15901 tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata acaatcttgc
    15961 caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat tatcataagt
    16021 actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag gaatgctgaa
    16081 cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa gtatcatgat
    16141 aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc ctgttaaacc
    16201 ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta cattgataaa
    16261 aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt ttttcgtatc
    16321 ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag aaaaaatgct
    16381 aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa tagtgcccaa
    16441 tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa taactcctac
    16501 tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc aatgtttttt
    16561 tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta tactactttt
    16621 atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt acaatttttt
    16681 agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc gatatttttt
    16741 aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat tattttagtg
    16801 agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt actttctttt
    16861 atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa cgtgttccca
    16921 atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt cacgcgatgc
    16981 aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat acagtcggta
    17041 atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat agatttgtaa
    17101 ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag gatattatac
    17161 agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa gcttgcttca
    17221 ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat attaacaaaa
    17281 gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc gataacgata
    17341 gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag agacacaaat taggcgtaat
    17401 tctaataaaa atcttagaat aaaaattttt actgatttaa tttagaatgt ttttaaaa
//
LOCUS       DACCWV010000053        17379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCWV010000053 DACCWV010000000
VERSION     DACCWV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.053"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            23..253
                     /locus_tag="IVW84_001496"
     CDS             23..253
                     /locus_tag="IVW84_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7917696.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            350..1651
                     /gene="murA"
                     /locus_tag="IVW84_001497"
     CDS             350..1651
                     /gene="murA"
                     /locus_tag="IVW84_001497"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7917697.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1664..1840
                     /locus_tag="IVW84_001498"
     CDS             1664..1840
                     /locus_tag="IVW84_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7917698.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1975..2271
                     /gene="yidD"
                     /locus_tag="IVW84_001499"
     CDS             1975..2271
                     /gene="yidD"
                     /locus_tag="IVW84_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7917699.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2335..2802
                     /locus_tag="IVW84_001500"
     CDS             2335..2802
                     /locus_tag="IVW84_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7917700.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2887..3771
                     /locus_tag="IVW84_001501"
     CDS             2887..3771
                     /locus_tag="IVW84_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7917701.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            3888..4121
                     /locus_tag="IVW84_001502"
     CDS             3888..4121
                     /locus_tag="IVW84_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7917702.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4209..4289
                     /locus_tag="IVW84_001503"
     tRNA            4209..4289
                     /locus_tag="IVW84_001503"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4243..4245,aa:Tyr,seq:gta)
     gene            4296..4367
                     /locus_tag="IVW84_001504"
     tRNA            4296..4367
                     /locus_tag="IVW84_001504"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4328..4330,aa:Gln,seq:ttg)
     gene            4883..5590
                     /locus_tag="IVW84_001505"
     CDS             4883..5590
                     /locus_tag="IVW84_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7917703.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5649..6293)
                     /locus_tag="IVW84_001506"
     CDS             complement(5649..6293)
                     /locus_tag="IVW84_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7917704.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            6420..6707
                     /locus_tag="IVW84_001507"
     CDS             6420..6707
                     /locus_tag="IVW84_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917705.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6815..7135
                     /locus_tag="IVW84_001508"
     CDS             6815..7135
                     /locus_tag="IVW84_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7917706.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            7453..8529
                     /locus_tag="IVW84_001509"
     CDS             7453..8529
                     /locus_tag="IVW84_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7917707.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            8526..9245
                     /locus_tag="IVW84_001510"
     CDS             8526..9245
                     /locus_tag="IVW84_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7917708.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            9373..9924
                     /gene="raiA"
                     /locus_tag="IVW84_001511"
     CDS             9373..9924
                     /gene="raiA"
                     /locus_tag="IVW84_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7917709.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10146..12680
                     /gene="secA"
                     /locus_tag="IVW84_001512"
     CDS             10146..12680
                     /gene="secA"
                     /locus_tag="IVW84_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7917710.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12806..13907
                     /gene="prfB"
                     /locus_tag="IVW84_001513"
     CDS             join(12806..12868,12870..13907)
                     /gene="prfB"
                     /locus_tag="IVW84_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7917711.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14012..14698
                     /gene="ftsE"
                     /locus_tag="IVW84_001514"
     CDS             14012..14698
                     /gene="ftsE"
                     /locus_tag="IVW84_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7917712.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14682..15575
                     /locus_tag="IVW84_001515"
     CDS             14682..15575
                     /locus_tag="IVW84_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917713.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15727..17175
                     /gene="cls"
                     /locus_tag="IVW84_001516"
     CDS             15727..17175
                     /gene="cls"
                     /locus_tag="IVW84_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7917714.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5813 a         2954 c         3792 g         4820 t
ORIGIN      
        1 aatcgttaga aaggacgtca ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt
       61 gagtcatttg gcgtttatct acttagcctt ctggtcttta cgttcgttac gcattgaaac
      121 ttttttcaga ccaatgcata atacgcaaat tagaatggca atcgtacttt tttcaatttt
      181 tttagggtac actgcaagta attttttctt agaggttatc gctttatgcc gaaatctttt
      241 cgttagcctg taaattttaa aaaaaggtac tattttaaaa gcaaggatgt tataatagaa
      301 aagattttga gtaagtgtat gctcattgga attttttgga gggaacaaca tggaagagat
      361 catcgtaaga ggtggcaatc aattaaatgg aacagtaaga attgaaggag ctaaaaatgc
      421 tgttttgcct attttagctg ctagtttgtt agcagaagaa ggtattacga cattagacaa
      481 cgttccgatt ctttctgatg tcttcactat gaatcaagtg attcgtcact taaacgtaga
      541 tgtagatttt gatgaacaaa agaatcaagt aacaatcgac gcttcaagac aattagaaat
      601 cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct
      661 acttgccaga aatggacatg caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg
      721 accaatcgat ttgcatttaa aaggatttca agctttagga gcaaaaatta ttcaaaaaaa
      781 cggctatatc gaagcaattg cggatgaatt gatcggtaac acgatttatt tagatttccc
      841 tagtgtaggt gctactcaaa atatcatgat ggctgccgta aaagcaaaag gaacaaccat
      901 cattgaaaac gtagctcgcg aaccagaaat cgttgattta gcaaacattt tgaataaaat
      961 gggcgctcaa gtctatggag ctggtactga aacaatgcgt attgaaggcg tggatcattt
     1021 acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc
     1081 tgctgcaatg acacaaggca atgtgctgat tgcagatgct atctctgaac ataaccgccc
     1141 attgatttct aaattgatcg aaatgggtgc agaaatcatt gaagaagaag gcggagtccg
     1201 tgtcatcgga ccaaaacata tcttacctac agatgtaaaa acgatgcctc atcctggttt
     1261 ccctacagat atgcaagctc aaatgacagc tatccaatta gttgcagaag gtacaagtgt
     1321 ggtgactgaa acagtgtttg aaaatcgttt ccaacattta gaagagatgc gccgtatgaa
     1381 cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg aattgcaagg
     1441 tgcagaagta tatgcaacag acttgcgggc agcagcagca ttagttttag ctggcttgaa
     1501 agcaaatgga atcacacgtg tgcgtaactt gaattatcta gaccgtggat attataattt
     1561 ccatatcaaa ctacagcaat taggtgcaga cgtcgaacgt gtagacatgg atcaaacttc
     1621 tgctgaaaaa acagcacaaa cgattgctta aggaagtggc tgaatgagta accaacgata
     1681 tatcttatta actttgataa agattttagt ggtgatctta ttgcttatcc ttctttttgt
     1741 ggccggaacg atgatcggat atggtgtcat cggtggcggc aatccattta aagtattcca
     1801 gccaagtttg tggattcata ttcgagactt ttttcattga taaataagaa tcacagtggt
     1861 tctagtcgat atgaagtgag ccatgaagca gaacatgata aaagaaaaca gaagggaacg
     1921 ttgacgttag tcgcgatccc tttttttagt aaaagaaaag aaggaatcta attgatgaag
     1981 aagatattga tttatttagt tagaggatat cagcgcttta tttcgcctgc atttccacct
     2041 agctgccgtt attacccaac ctgttccaat tatatgataa aagcaattca aacccatggt
     2101 gcgctaaaag ggacggtaat ggggactgca cggatcttac gctgtcatcc ttttgtcaaa
     2161 ggcggtatcg attatgtacc attgacattc cgattaacgc gaaataagaa agataaaaaa
     2221 gaaccagatt ataaaaaggt tgtgaaaaat acgggtagca ctgagcgata aagaaaaaga
     2281 ggaaatttat tgtcaaagta acaactccgc aactacttat atgggggaca aaaaatgatc
     2341 gaagaactaa acaagaaaga tttaccatgg tccttgttat tagatgcaga tcctgataaa
     2401 gaaaaagttc aagtatatgt ttccagaggg agtggctgga tatggaaaga aaaagaaaaa
     2461 acaataggtg tactgatcta tgtggcaagg gaaaaagaat acgaaatcgt caatgtagca
     2521 gtaaccccaa cgtatcaagg aaaggggatt ggcggaaaat tacttgagac agcttttcag
     2581 aagctttcac agctagcttc aaaccaaact cggatcatta tacgtactgg cactactagt
     2641 agcgctgcgt tacacctcta tcaaaaaatg ggctatgtgg agattggtcg agtcaaagac
     2701 tattttatcc atcattactc tgagcccatt tttgaaaatg gagatcaatt aagggatcaa
     2761 gtgattttag caattcactt acctaaaaat agctttaact aataaacttt caattcgaga
     2821 aaatgctttc aaatgctaaa tagatacatt acaatagctt tagcaaacaa aaaggagaat
     2881 atttttatgg gaatattagt ttttgatttt ggtggatcgg ctgtcaaata tggttgttgg
     2941 gatggcaaag agattaaagg taagggacaa tttgcaacgc ctgaatcttg ggaagaaatg
     3001 aaaacgcaat tatttcaagt ttataaaaaa atactcgttt cttttgaagt cgaaggaatt
     3061 ggaatcagct ctcctggagt agtgaataat gagaaacagg tgattgaggg aatcagtgcg
     3121 attccttata ttcatggatt taatattttc cgagatttgg aaactctgtt tcaactccca
     3181 gtaacgattg aaaatgacgc caactgtgcc ggaatggctg aattttatga aggtgctgcc
     3241 cgtgattatc aagatgttgc ttttgttgtg gcaggaactg ggattggcgg ggcgttgttc
     3301 acgaaaggac aaataaataa aggggcacat ctttacggtg gagaatttgg gttgatgttc
     3361 ttagatggcg ataagacatt cagtaaactt ggtacagcgg tacagatggc ttggcgttat
     3421 tgtgaacgca aaggtctcga taaaaatgct tataccggaa aagatgtctt tgaattagca
     3481 gaaactggag atgcaatagc aaaagaagaa gttgagagtt tttatacgta tcttacaaag
     3541 ggattattca gtattcaatt ttcgtttgat ccagaagtga tcgtattagg cggcggggtt
     3601 tctgcaaaag aagggctcat tgatgagatc aaaagccgaa tgaagaagct gacagaacaa
     3661 ttcgatctgc atgatttcga accgcagatt ttattatgtg agtacagaaa cgatgctaat
     3721 cttataggtg cagcagcaaa ctatctcgct gtacgtcaag gagagaatta agagctttag
     3781 agaaggtgca gcttgcataa aagaagagaa tgaaatgaat aggagatttt tacctaagga
     3841 atgttttatt tgctgcagta tgataaaata tctgagaggt gaaaatgatg tcaaaaaaaa
     3901 ataaagatat tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg
     3961 aagtcactca gttatttata gggaaaaaaa tgattggtga gatcttagcc tatgggccaa
     4021 aagaatatga aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg
     4081 cgatagaaac agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt
     4141 tcaataaagg gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca
     4201 aacctttcgg aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg
     4261 gttcagtggt tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca
     4321 agggactttg actccctcat gcgttggttc gaatccagct accccagtat accacaatcg
     4381 taagctctcg ttagttcgag agctcttttt ttttgctttg ctaccttgtt ccttgaagat
     4441 tctgctgaat taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg
     4501 gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata
     4561 caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg
     4621 cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca
     4681 taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta
     4741 aaaaattgtt tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat
     4801 ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga
     4861 aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga
     4921 ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc ttccttcaga
     4981 aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa
     5041 cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga
     5101 aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa
     5161 tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat
     5221 ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc
     5281 agatgatgtc cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta
     5341 cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa
     5401 gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta
     5461 tttagaggta aaacgaagag atgcagttct tcgtttgcgc cagatttctt attttgaaga
     5521 cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt
     5581 ggaaaaataa aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc
     5641 acctgttttt atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca
     5701 aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtt agcacttttt
     5761 tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa
     5821 tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag
     5881 atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca
     5941 tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg
     6001 actactgcag ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc
     6061 gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg
     6121 atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa
     6181 ttttttgctt ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg
     6241 atctcaatct cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca
     6301 cctctttttt tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt
     6361 aacggttgcg caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga
     6421 tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct
     6481 ctcaagtaac gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc
     6541 ccaaaacagc aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag
     6601 gtgcctatgg ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat
     6661 atgtcgtgtt caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt
     6721 agaaaacgtt gacaaatttc tttcttttta tataataaag taacagacgc gaagaaaacg
     6781 tttgcgtagt ttgttttaaa aaggagagaa aaatatggct aaaaaaacaa tcatgcttgt
     6841 atgttcggct ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc
     6901 aaaaggaatc gactctgata ttttcgctgt gtccgcttct gacgcagata acaatcttgc
     6961 aaataaagac gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt
     7021 tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg
     7081 tatgatgaat ggcgaaaaag tgttagatca agcaatcgcg ctcataggtg aataaataga
     7141 acagtataac tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt
     7201 tttagggcga ataatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattaaa
     7261 gcaatagact gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt
     7321 ttgggcaact ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgttggacag
     7381 cttgcagatt acggttattt ttgtacacat tgtctatatt tagggtgatg cgacactcaa
     7441 caagatttat atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg
     7501 acaggacagc ttactgaaaa acaaacggaa atcgcaaaga gaatactttg ccattcagaa
     7561 aaaagacatc atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct
     7621 attttagtgg aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat
     7681 gtttgtaatg aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg
     7741 ctccatggga atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat
     7801 cagctatacc gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt
     7861 ccttataacg gggatgctgg gctagctcac gctgtgcaaa cagcaatcag atcaaacggc
     7921 cgattcatct acttaagtgc cacgcctgat gagaagttac taaaggaaat caagcagaca
     7981 tttgagctcc acaaacttgc cttgcgcttt catcggcgct tgcttcctga acccgtgctt
     8041 gttttcatga ataattggcg acaaaaatgt ggaaacaaaa agaaaatcaa ctctttggtg
     8101 cggatcattc agcaactaat caaagaaaac cgtatccttt tattttgtcc aagtattacg
     8161 atgatgcatc gtttaaaaat cacactgcaa gaagcttttc cagaatatga gataacagat
     8221 gtttcttcac aagacgatca acgggcaaag aaagttcaga aaatgcgaga agcaatatac
     8281 gagatactgt taaccacaat gatcctagaa agaggagtca catttgaaag aatctctgtc
     8341 atcgttttag gagcagatca tccagttttt actaaatcct ctttggtgca aatcgcagga
     8401 agagctgatc gtaaaggtcc ttatacgaac agtcgagtct attttttcta tgaagaaaaa
     8461 acttcagcta ttcgaaaagc ctgtcaggaa atcaaagaaa tgaacgaaga agggaagcag
     8521 atgctgtgag atgtagttgc tgcggtaaag acatcattca aaatttaaca ttcagcgaga
     8581 tcatcggagt gtcgaaatct agacttcctc agacatgttc agtttgccgc gcacaattcc
     8641 atttattaga agcaaaaggt tgtgcaggtt gtcaaaaacc aatccatttg aaatacgagg
     8701 agacgaaaaa gaaagcaaat actagtttcg tttactgcaa cgactgtcaa gaatggaaga
     8761 agaaatatcc tgcatatgat tttcaacacc gcgccttttt tacgtacgat gaaaagatgc
     8821 aggcttggat caatcggtac aagtttatgg gagatatccg attagcaggg acttttgcat
     8881 ataaatggac tcagataaaa aaagattacc cggcatatat ttattgtcca atacctttat
     8941 ctgaaaaaag gtggctagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
     9001 aaattccttt tcagatgctg ctcaaaagag agcaacatct gcttgctcaa gcaaaaaaca
     9061 cgcgaaagga aagattggag atgcctcagc cttttgagct tgccgtacct ctttctaaga
     9121 tacaacataa aaaaatattg attattgacg atgtctatac aacaggacag acgctttttc
     9181 atgcagcaga ttgcttgcag aacgcacagc cgaaaatcat ccgaaccatt tctttggcaa
     9241 gatgaatcag atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata
     9301 taatagaatt aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa
     9361 ggggtaattt ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg
     9421 attcgcgact acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc
     9481 gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta
     9541 acgatcccat taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca
     9601 agtatcgatt tggttgttga caaattggaa cgccaaatcc gcaaattcaa aacaaaaatt
     9661 aatcgtaaat cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa
     9721 gaggacgaaa acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg
     9781 aaaccaatgg atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt
     9841 atttttgaag atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa
     9901 tacggactga ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca
     9961 gctactgaca gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat
    10021 aaattatgaa gttcttactt taatacgctt aagatatttc aattatctat aactaatgat
    10081 aaaatgaaaa gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac
    10141 aacgcatggc caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt
    10201 tagatagcat tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg
    10261 aacaattaca ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag
    10321 atcaattact tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattaggtt
    10381 tatatccata ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg
    10441 aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc
    10501 tgactggcga aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga
    10561 cggaaatggg cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa
    10621 aatcttcgga tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg
    10681 aattaggatt tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac
    10741 gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa
    10801 cgccgctgat catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca
    10861 acttcgtgaa acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa
    10921 tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat
    10981 acgacattga aaatactgca ttgacccatc acttggatca agcattgaga gccaactata
    11041 tcatgctttt agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt
    11101 tcactggtcg gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag
    11161 ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga
    11221 actatttccg tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag
    11281 aagaattcag agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta
    11341 tccgtgatga tcgtccagat ttgctttacc caacattaca aagcaagttc cacgctgtcg
    11401 tgcaagatat caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg
    11461 ttgaaacatc tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc
    11521 tgaatgcgaa aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg
    11581 ctgtgacgat tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg
    11641 taagagaact tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg
    11701 ataaccagct tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca cagttctact
    11761 tgtcattgga agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag
    11821 atcggatgaa gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag
    11881 tagaatctgc acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac
    11941 aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa
    12001 tcatggaaga aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc
    12061 gtgtagtaga tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg
    12121 attttgccgg cagtactctt gtccatgaag atagcattac tcttgcagat atcgaaggaa
    12181 aaacagctca ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca
    12241 aagcggctca actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc
    12301 gtgttgtgga ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa
    12361 tcggcttacg tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata
    12421 agatgtttga agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag
    12481 ctgagatccg tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag
    12541 aagacggaga tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga
    12601 ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc
    12661 atggaaaaaa tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa
    12721 gaattcgtct caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa
    12781 aagtatagtg aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga
    12841 aatgcaggaa aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag
    12901 acatcgcgga agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg
    12961 cacaaaaagt gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc
    13021 ttgccgaaga actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg
    13081 cagcaatgca agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt
    13141 atgaattatc tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc
    13201 atccaggggc tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata
    13261 cacgttgggc agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg
    13321 aagcagggat caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa
    13381 aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac
    13441 gtcatacttc tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag
    13501 aaatcaatcc agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc
    13561 acatcaataa aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag
    13621 ctagtcaggc acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag
    13681 ctaagcttta tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag
    13741 aacaaaaaga aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta
    13801 tggtaaaaga ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg
    13861 accttgattt atttattgac ggctatttaa aaatgcatct acaataagaa cccattgtta
    13921 aattgaaata aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc
    13981 taaagactga aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa
    14041 gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt
    14101 tgtctatgtg gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg
    14161 agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa
    14221 aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt
    14281 attaccaaga aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa
    14341 acctcgagat atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa
    14401 agttcgagtc tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg
    14461 ggcaatcgtg aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc
    14521 agaaaactca tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt
    14581 cgtgatggct acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat
    14641 cgaaaacggc cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa
    14701 cctttttccg ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag
    14761 ttgcttcagt cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta
    14821 tcatgaatgc aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg
    14881 tagatatcgg tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag
    14941 atcatgttaa aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg
    15001 aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg
    15061 tagtaagtgc tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga
    15121 agaatgtttc tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg
    15181 gtgcagttcg gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc
    15241 tcatttcgaa cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc
    15301 gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga
    15361 tcggtctgat cggtgcaatt cttccaattt tgatcatcac atttggttat gcatggtttt
    15421 tccaattgat caatccatca ttattacgtt cccattattc attgattcat ccacaaaata
    15481 tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct
    15541 cgatcatttc tatgagacgt ttcttgaaat tctaatacca caatataaat aaaatgagaa
    15601 agttggacag tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg
    15661 cttttttccc tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt
    15721 gaagccgtga aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg
    15781 ctttccttta tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg
    15841 ctcgttttga tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg
    15901 atttccaaag agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa
    15961 attgaagaag acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa
    16021 gtagatgtga aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact
    16081 aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat
    16141 atcaagcagg cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta
    16201 ggtgaagaag ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc
    16261 cttcttgatg catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa
    16321 gcaggcggag aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc
    16381 aattatcgca atcatcgtaa aatcgtagtt attgatgggg aaatcggata tacaggtggg
    16441 ttcaatgttg gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat
    16501 catttgcgta tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg
    16561 aattctcagc atacaaaaga agtaggctat tctcccaaat tttttccgtc cattcacact
    16621 actggtgata tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc
    16681 aaaatgactt atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct
    16741 tattacatac cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta
    16801 aaagttcata tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat
    16861 tctttcgctg cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt
    16921 attcatgcaa agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc
    16981 gatgttcgtt catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg
    17041 gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa
    17101 gaaatgtatg aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt
    17161 tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg
    17221 tcctttttta acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg
    17281 agcgaaatta actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat
    17341 atgctattaa aagagacaca tttcaaagcc agtatagca
//
LOCUS       DACCWV010000054        17366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCWV010000054 DACCWV010000000
VERSION     DACCWV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.054"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(127..672)
                     /locus_tag="IVW84_001517"
     CDS             complement(127..672)
                     /locus_tag="IVW84_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7917715.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(684..1376)
                     /locus_tag="IVW84_001518"
     CDS             complement(684..1376)
                     /locus_tag="IVW84_001518"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7917716.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1395..1679)
                     /locus_tag="IVW84_001519"
     CDS             complement(1395..1679)
                     /locus_tag="IVW84_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917717.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1692..2255)
                     /locus_tag="IVW84_001520"
     CDS             complement(1692..2255)
                     /locus_tag="IVW84_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7917718.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2483..3145
                     /locus_tag="IVW84_001521"
     CDS             2483..3145
                     /locus_tag="IVW84_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7917719.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVILVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3269..4465
                     /locus_tag="IVW84_001522"
     CDS             3269..4465
                     /locus_tag="IVW84_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7917720.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4566..5942)
                     /locus_tag="IVW84_001523"
     CDS             complement(4566..5942)
                     /locus_tag="IVW84_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7917721.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5979..6845)
                     /locus_tag="IVW84_001524"
     CDS             complement(5979..6845)
                     /locus_tag="IVW84_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7917722.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6934..7959)
                     /locus_tag="IVW84_001525"
     CDS             complement(6934..7959)
                     /locus_tag="IVW84_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7917723.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7977..8651)
                     /locus_tag="IVW84_001526"
     CDS             complement(7977..8651)
                     /locus_tag="IVW84_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917724.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8835..9728)
                     /locus_tag="IVW84_001527"
     CDS             complement(8835..9728)
                     /locus_tag="IVW84_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7917725.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9802..11499)
                     /locus_tag="IVW84_001528"
     CDS             complement(9802..11499)
                     /locus_tag="IVW84_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7917726.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11492..12805)
                     /locus_tag="IVW84_001529"
     CDS             complement(11492..12805)
                     /locus_tag="IVW84_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7917727.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12805..16842)
                     /locus_tag="IVW84_001530"
     CDS             complement(12805..16842)
                     /locus_tag="IVW84_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7917728.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16855..17313)
                     /locus_tag="IVW84_001531"
     CDS             complement(16855..17313)
                     /locus_tag="IVW84_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7917729.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4874 a         3896 c         3019 g         5577 t
ORIGIN      
        1 tattaacgta aaaagaataa gtttttttaa aataacgtat aaaaaagagc ataaaacaaa
       61 gtcgtttgtt ttatgctctt cttttattat tttgtgcttt ttattttatc aaatcacttt
      121 gtttttttat aaaatttctg cgcctagtgt ggactcaaaa tgtctcaatg cccattcatg
      181 tccaacggga tcgaaacttg ccacggaatc tttgaaaata tggagtttgt atcctagatt
      241 atacgcatcg actgctgtat gcaacacaca gatatctgta catacgccgg tcaaataaat
      301 atctgtgatc tggcgttctc gcaaacggat gtctagatct gttccactaa aagcggaata
      361 atgccgtttg tctatccaat aaacattttc ctcttgctca tagtcttggt ataaactttt
      421 taaagagccg tacaattccc gtccacccgt tcctatcaca ttatgcggag gaaataagcg
      481 gttctccgga tgaaaggaat cttttggatc atgcgcatca atggcaaaaa cgacaaaatc
      541 cttttgatca ataaacttct ttgtatgagc agtcagcttt ttttcaatta cttgtcctgc
      601 tgtcccagta gtcagctttc catcctctgc aacaaaatca tatgtgtagt caatcgagat
      661 caatgcgttc atcttagtcc tccttatacg agatgtttga caaattcgat caccatttct
      721 gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc tttgtgtcgc cgagtgatct
      781 gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat tgaactgacg agctgtttgt
      841 ccaacagcgg ctccttccat ttcgcaagct aatgcttcag ggaaattcgt taagatttct
      901 ttgattttat ctggcgagtc gacaaatgta tcacctgtaa cgatcaatcc ttcttttgcg
      961 ttcaaattcg ttgcttttgc tgctttaacc atttcactgc gcaaatatgt gcttgcttca
     1021 taatatagag gcattcctgg taattgtcct gggttgtagc caaatcctgt tgcatctgca
     1081 tcaaaataag ctactttgtc tgatataacg atgtcaccta cttgtaagcc ttcaccgatt
     1141 ccgccagctg aacctgtatt gatcaccatg ttcacgccgt attgttggat caacagactt
     1201 gtggtaatag atgctaagac ttttcctata ccggaacgta cgacgatcac ttcgtggtta
     1261 cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc atgataatgg ttcgcttagg
     1321 ttctctctca aaatttttac ttcttcttcc attgcgccga taataccgat tttcatcttt
     1381 ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa ccacacataa cattaggata
     1441 acaataataa gagatttcat caaaaaagag ttccatttgg tcgtcttttc ccgttcgctg
     1501 atccttgttt tcgtgattgg tttattcttt ttgctttctt tacgataaaa gctggcaatc
     1561 tttttttctt cttgctgata agctgtttct gcttttcgct gtttttttaa tgactcgctt
     1621 gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa taagcggtcc tttggccata
     1681 agccgatccc tctactttcc tttggtcaat agttcccagt attgaatcgc ataaatcgtc
     1741 tttgcatcac aaatcaactg atctttcatc gcttgttgcg cctcttcaag agttagatga
     1801 tacaactcca gtacttcgtc ttcatcttgt gctaaaggat tctcgacctt ctctacccct
     1861 tgtgcatgat aaatatgaag gacttcattt gcaaaacctg gtgaaagata catcgaagta
     1921 agatggctca gcgacttcgc acgatagcca gtttcttctt ctaattctct agctgctgtc
     1981 atttcgggat tttgtccttc tcctggatcg atttttccag ctggaatctc caaaatcact
     2041 ttttctaaag gttttcgaaa ttgtttgaca agaagcagac gatcttgttc gtcaaaagca
     2101 atgatcccga caccgccagg atgaaatacc agttctctct tcgaagttcc tccatctggc
     2161 aaacgaactt cgtctacagc aacatcaata atttttccgc tatagatttc tttgcgactg
     2221 attgtttttt cttcaaattg ctcattgttc agcatatctt ctcctgcttt ctttgtaatc
     2281 ataactgctt ttttttcatt tttcaatttt tctcaaaaac tccttattag tttaccatag
     2341 ataaacctta gattttctct aaatatagaa aatccttctt tagtccgata ctctttctag
     2401 tagtccgtat gctaaaataa gtatgtaatc gaataacgaa aagaattcaa tactcaacga
     2461 taaatgacat ggagggttta ttatgataaa acgaaattgg ccgtggcttc tagcattggt
     2521 cttgctattt ctctttgtcc gaaacaatag tttttctttt gttctaggac ttttattgat
     2581 aggtgtaatt ttagttttgt ggggactctt tggttcgcgc aggaaaaagg gaaaacaaga
     2641 agaaattcct gcactgactg acgaacttga aactcactat gaaaaaagcg gtatgacagc
     2701 gaatgaaatt tcattttttc gacaaacaat gaatcaaaca aaaaatgaga tcaatcaatt
     2761 gcaaatgaat atgcagcaga cagccaaact taaagcaatc gatctccgcc atgatccagt
     2821 caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa ccaaaccgac tgcatgaagc
     2881 aagtcagttt ctttatacgc atctgccgaa tctagttgat ttgacaaata agtataatga
     2941 gatcaatgac catgagatca aaaacaaaca gacctatgaa aaactagaag aaagcgcgca
     3001 gatcatcgac cagctgtcag ctttaatcgc acaagattat caaaaattcg ttgctgagga
     3061 tctagatgat atcgacgtag agatatctgt tgcgaaacag agcttaaagc gtgataataa
     3121 atatgaacca gaacaaaagg attaactctt ttgttcataa aagaagactc ggaacattca
     3181 gatgatgtct ggctgtttcg agtacttttt ctaaagtaga gacattcatg taagaatcga
     3241 gaacagattc aacaaggagg aattttttat ggatcataaa ccgactgaaa caaaagatgt
     3301 tacaccagtt aacgatacac ttgaagattt attgaacaac ccgttttcaa ctccaacaga
     3361 tacgctgacc acttcacaac aagcggagat tgatcagctc caagagcaac agacagctgc
     3421 tcgtttaatt gacaaactac cggcggaacg ccaagaacaa gctaaacagc tagcagcaaa
     3481 aatcgatgct aatgatgcac agtctgtcat cagctacgga tcggctgccc aagcaaaatt
     3541 aagtgaattc tcacaatcga tgttgaatca tgtgcaagca caagatattg gtcctgtagg
     3601 agattcctta actgaactga tgtatcgtct ccaagaagcc aatcctgacg aactgcgtgc
     3661 cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc aaacaatcga tctatgaagt
     3721 gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag atttcagtga aattagataa
     3781 agaaaaagac ggtctgctaa aagataatct gatgttagaa cagctttacc agaaaaacaa
     3841 agactacttt gatgcactga atatttatat cgctgctgga gaattgaaaa tggaagaact
     3901 tcaaacaacg atcattccag aagcgatgaa aagagcagaa gaaactggcg atcagatgga
     3961 tgttcagatt gccaatgatt acacgcaatt cctagatcgt ctggataaac gtacgcatga
     4021 tcttcgctta gctagacaga tcacgatcca gcaagcacca caaatccgtt tgatccaaaa
     4081 cacaaatcag gccttggctg aaaagatcca agcatctatc gctacagcca ttccgctgtg
     4141 gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa aaagatgcag taactgctca
     4201 acgccaagtc tctgaaacaa caaacgactt attgaagaaa aattcagaaa tgctgaaaat
     4261 ctcagccatc gaaacagcga aagaaaatga acgtggaatc gtggatatcg agacccttca
     4321 aaccactcaa aatgatttga tcgaaacaat ccaagagaca ttgcgtatcc aaaaagaagg
     4381 aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac atggaagaag atctgaaaca
     4441 aaaactgttg gatttaaccc aatagatcac ctttcgcttg ttactaaaac aaacgcccaa
     4501 caacagttta ttacaactgt tgatgggcgt ttttcttatc tttcttctaa cttctccatt
     4561 ggagtctatt tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt
     4621 ccaataacca ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat
     4681 acaatacaag tcaaaatgga gacgattggc aatagcggaa cgaatggcac tttgaattct
     4741 ccagcttttg gtttgccaaa catttttctc aacttcagta agcctagact aacaagcatc
     4801 aaataggcga gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg
     4861 gcaaagaata cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt
     4921 tgatcgattt ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa
     4981 cgagcaagtg aatacatcat cgagatcgtt accgtcagta gcgttaaaat tgcccctaca
     5041 gcgatcaccg cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca
     5101 ttgactccta gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa
     5161 aggacagtag caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt
     5221 ttgacttcct cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga
     5281 gctgcaccat ctagcacacc ggaaaaacca aaaggcatga atggctgcca gttatcaggt
     5341 ttaacgaaaa atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc
     5401 atggcattat tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat
     5461 atcactacga gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca
     5521 gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac
     5581 catccggaag caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc
     5641 agccaggcag caaattcacc aaatacaaca tacagatagg catatggccc accaatcact
     5701 ggcactcgcg aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct
     5761 aagacaaatg aaagtgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc
     5821 acaaatattc ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt
     5881 agttcttttt tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc
     5941 attgaagttc ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct
     6001 aaaaggatac cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt
     6061 ttcgtttttc tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt
     6121 tgtgaaagtg agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg
     6181 tttcctaagc cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta
     6241 actttacgga aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata
     6301 attgacatcg catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc
     6361 gtagagaaga tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct
     6421 atttttcctt cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa
     6481 gcaataatac cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc
     6541 gcaatcgtat tcaaaattaa ttgaaaacca gtagacaatg gtagccctac agaaacaccc
     6601 atgtacttca ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa
     6661 aatccgatca agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa
     6721 gaaaacagaa atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt
     6781 ttaccactta ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt
     6841 tccataacca atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca
     6901 acacaagcac tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa
     6961 gattgggaaa aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg
     7021 gatacctaat ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt
     7081 tttgctaagt cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc
     7141 cgctgggata ccgatccctg aatctttaac tgaaaggctg actccttgcg cttccttttc
     7201 tatgctcaca atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg
     7261 gttgaagata aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacgat
     7321 ctcgtagcga atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat
     7381 gattcctttt agtccatatt cttggatgag gtagtcccgc gagaaactgt cgagtcttgc
     7441 ataatacaaa acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt
     7501 atacttttga tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag
     7561 tggcactttg atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact
     7621 gattgctgat tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac
     7681 cagtttttct tcttctgtcc tcccaccact caaataattt tgcaaagggg aagttttttg
     7741 tatgtcggct aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa
     7801 aatcagaaag acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat
     7861 gtttggcgcc agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat
     7921 caatgtccac tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag
     7981 ggaacgatat atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg
     8041 atttttcggc gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg
     8101 tcatcccaca gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc
     8161 aaaataaata atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc
     8221 atacttccgt tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat
     8281 tctgtgtagt tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt
     8341 tggaaatgtt ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata
     8401 ttcgtattcc tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga
     8461 caccaataat agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct
     8521 tctctttcaa agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc
     8581 catttctcaa gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg
     8641 atttttgcca taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc
     8701 ttgtgaaaca agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg
     8761 aaatatgcga tttaatcgcg tttgtctctt ccaagcagct taaatttatt atgttattct
     8821 taataattgg tctattatct gttatttttc tttcgttgtt tttgtctttt aaattttcga
     8881 taacaccaat aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat
     8941 cgataaagat ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga
     9001 actcgtcttc ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata
     9061 agtgtgacta gatcctgctt ttctctaatt gctaatgtat ttgtttttgt tggaaggaca
     9121 acaattttct caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg
     9181 tcatcttgct ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg
     9241 gcggtaatca cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact
     9301 gtcgctcctc tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg
     9361 attttcggaa tatagatggc tccgatcata tgttgttcgt aatattcttt tcctggattt
     9421 tggacatttc taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact
     9481 gatgtatgag caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt
     9541 tcttctttat cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa
     9601 ttgctgagag catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc
     9661 actgccataa ataattgtaa taaaagactg atttgggttt gaggtctatt acctattttc
     9721 tttttcattt tcttcacctt cttatttaaa aaaactgcag caacctttat aaaaccgatt
     9781 gctgcagctt atttttgttg cttatacttc agacttcatt ttagatttac ggtaccagct
     9841 gtacgctcca atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc
     9901 agtagcagga agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac
     9961 tcctacgatc tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc
    10021 tgcctcacct gttggaagta cataaccttc tggcgccatg gtctctttca tttgataggt
    10081 tccataatct aacccttgaa cctcaaagta tccatttcca tctgcgacaa attctgttgc
    10141 ttgtccgctt gttgcccact caattctatc tgctcctgat tcataaacag ctaaaggcga
    10201 ctccccttta taaaatattg cgtattcaga acggtcttca ttccacaatt cgaatcttgc
    10261 atctttaagc aattcactgc ttgatgcatc atatttttca aactttcttc cacctgttgt
    10321 gactttctct ggcggctcta ctccttcttc ataatcaaat tcatcatttc gtccgatttc
    10381 aataaccgct gtgttgttat ggaaatcatc cgcttctagt tctgtggaaa ccttcaaatt
    10441 atacgtgatc gttaaatctc ctccacgtgc atctgctaat ctacctaaag tatctacttt
    10501 ggctactcca ttttcttctg tcaaaatcgt cacgacaaac ccgttgctta ctacttctac
    10561 tatataatct tgtccttttg ttaaaatcgt ctcgccggct cgcacttcta tttcctgatc
    10621 ataataagtt agaccaactg taggcgtatc tgtgattttg taatttttga taacagcgct
    10681 tccgttttca aggacagaat cgatgaaata aggaataggt gctgtgatcg tgtaagaagc
    10741 cgtttttcct ctttctagat caatataaga aatcgtttct tgttctccag cttcattaat
    10801 aatcgttact tgtctaaggt cgctttcctc tacattcatc tgtttatctg cttctctgat
    10861 ttcgtttttc ggatacaaat gcacatctgt attgtatgtg tctttccatg tagaaccatc
    10921 ccacacgcca tccaccatat caggcatcat cactggcatc ataacaacca ttggagacgc
    10981 gattctggaa atatgagctg gcgaataagt ttctaagaaa agatagactt tatctctaga
    11041 agaaggtgct tcttccgtca cttggacggt agagaatacc gcttctccag cttcattcgt
    11101 tgtttgtttg tcaatacttg tcagtcgata ttttaaaaac caactttctg tgtgccgttt
    11161 ttgaatccat tcaatcgctt cggcagaatt aagaccgtct tctcgttccg gtgttaaagg
    11221 attatctcga atcaaatcgt aatatacttc cgttacgtca aacaaatcaa aatcgactcc
    11281 tggaatattt tcttcaccaa aatccgtaga aaccaatccg ctattcggtc gttcttccgg
    11341 aacagatgag aaaccttttt tgtgtaatgt aatcgtcact tcacttggtt tttgtgcgac
    11401 ttcttcggct ttgactagtt ttccgttccc gaaaatccct attcccgcag taacgagtaa
    11461 cagtacactc attcccgtga caatcatttt tttattcatt tttttctccc cgttcttttt
    11521 ttattaataa aacagcacct aacaataaaa gaattcctag taaagaaatc aacgttcttg
    11581 cttcattcgt ttgcgggaat acggaccatt ttcctgattg acctggattg cttggaccac
    11641 tcggctcttt cggtttgtcc gttgtttctt ttacggtctc gttataaaca cgtggtgttg
    11701 cggattcata ggcagagact ggaactttcc cggattctaa ttcagccatc tcctgtccgt
    11761 taactactac atattgtggt tggccttttg catccaccca aaattcaggg atgatgacct
    11821 cgattctctt acttgcttca tccatctcgt aaccttccac accttttaat tcttcaaaat
    11881 aataggtgcc agaaggcaag aaacgttgtc ctgtcgtaac aaggccctct ttatccgaaa
    11941 tgaatttgct tacatttttg tcattcaaag gatcattgtc tgctggtttg atccattgat
    12001 ttttcaagtc attagcagga gccatatcta aataatattt ccccccttct atcatctgat
    12061 atagagcaaa aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc
    12121 catacttgaa gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct
    12181 cttgcagttc ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga
    12241 tagctgtctg atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa
    12301 tcaaataaac tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt
    12361 ctcctaatcc gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc
    12421 tttcttctgt gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga
    12481 attctttagg agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt
    12541 aaatggcaaa tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc
    12601 ctgcttcctc agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat
    12661 cgatatctcg aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt
    12721 ctgccgacat cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa
    12781 gcatcaaaca caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa
    12841 caaaccatag gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa
    12901 tcgtccggat cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg
    12961 taatctgatt cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt
    13021 ctctgcttct tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc
    13081 tggaactacc cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa
    13141 gtaacctgga agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc
    13201 ttccaacaag tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc
    13261 ctcgatgatt tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc
    13321 aagacgtcgg ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc
    13381 atcttctaag gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc
    13441 agtttcgtct tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa
    13501 ctggaacact tgatcagata gccgataccc atttggtgcg acagattccg agacctcata
    13561 ttcaccaata gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc
    13621 tacggcacga gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa
    13681 cggattacca gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc
    13741 gaaacctgca ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg
    13801 ataatcaaac gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt
    13861 atacgtgaga tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc
    13921 attatcgtaa agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg
    13981 gttctggctc tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata
    14041 aacaaatgaa tctggctctc cgattggatg aataactttc tcaccatcca cgattacata
    14101 tttcccgtct tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt
    14161 cacaccagaa agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc
    14221 ataaagttca aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat
    14281 attcatgtgg attggcttga attcatttcg catttccagt ttgacataca actcatcata
    14341 ctctgtgcct tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata
    14401 ataggaattc ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc
    14461 tacaagctcg tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa
    14521 ttgatacgtc tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa
    14581 tatgtcgctg actgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc
    14641 tattccgtcc gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt
    14701 ttggtcatat aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct
    14761 gacttggaaa ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg
    14821 tccagtagtg atatcattct cataatcctc caggttctga atataggcca tattgttcac
    14881 tgtgcaaaaa tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac
    14941 catttcccct tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac
    15001 atacgttgtc ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca
    15061 tatttctggc tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat
    15121 atctgccgat ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt
    15181 ggcttgctgg atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc
    15241 attcttgttg ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat
    15301 atttttcaat acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc
    15361 gattcctaaa ccaatggaga agatacttgg cgggttctct tgatacttgt ttttcagcca
    15421 atgatgataa gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt
    15481 cgtattgtta ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt
    15541 tacctctcca taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc
    15601 ataggtaact ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt
    15661 tctgaacaaa acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca
    15721 agctccatta gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt
    15781 cccatctccc acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt
    15841 atacaatgtt tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt
    15901 acgatttaat gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa
    15961 gatgtccgaa ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac
    16021 cataccgatt cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac
    16081 tgcctcaatc aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat
    16141 actcgctgac ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc
    16201 cccgatgaca tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga
    16261 tgctgtgctg cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc
    16321 caagtaactg ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac
    16381 gtttctatct tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca
    16441 gttcgctccc ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga
    16501 gcctttgtct ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata
    16561 gtcattcgtt gtaaacttcg ggtagcgatg ctgttcgttt tcaacttctc cgctaggata
    16621 gatatattca taggggtctt gcgtagctgt tggcccaaga cgctccaaaa taccgttgaa
    16681 taactggcgt tgaatgcgct ggagtgcttg accgctgggt acgattggtt cgtccagctt
    16741 ttccatctca ttttcttcgc tacgttgaat ttcagcggaa tcttctgtat cagtttcatt
    16801 ttcttgtaat tcttggttat ctattctttc atcaaccacc acttgaagag gtagttaccc
    16861 ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt
    16921 tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc
    16981 tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc
    17041 ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc
    17101 aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc
    17161 atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc
    17221 cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac
    17281 taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt
    17341 gtcttctttc acaacaatca acaaaa
//
LOCUS       DACCWV010000055        17328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCWV010000055 DACCWV010000000
VERSION     DACCWV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.055"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(307..1440)
                     /locus_tag="IVW84_001532"
     CDS             complement(307..1440)
                     /locus_tag="IVW84_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7917730.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="IVW84_001533"
     CDS             complement(1431..2099)
                     /locus_tag="IVW84_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917731.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="IVW84_001534"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="IVW84_001534"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7917732.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="IVW84_001535"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="IVW84_001535"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP7917733.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="IVW84_001536"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="IVW84_001536"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP7917734.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="IVW84_001537"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="IVW84_001537"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP7917735.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="IVW84_001538"
     CDS             complement(5475..6248)
                     /locus_tag="IVW84_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7917736.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="IVW84_001539"
     CDS             6413..6892
                     /locus_tag="IVW84_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7917737.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="IVW84_001540"
     CDS             6949..7251
                     /locus_tag="IVW84_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7917738.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="IVW84_001541"
     CDS             7273..8730
                     /locus_tag="IVW84_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP7917739.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9323..10075)
                     /locus_tag="IVW84_001542"
     CDS             complement(9323..10075)
                     /locus_tag="IVW84_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7917740.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10290..10526)
                     /locus_tag="IVW84_001543"
                     /pseudo
     CDS             complement(10290..10526)
                     /locus_tag="IVW84_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10536..11494)
                     /locus_tag="IVW84_001544"
                     /pseudo
     CDS             complement(10536..11494)
                     /locus_tag="IVW84_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11700..13382)
                     /locus_tag="IVW84_001545"
     CDS             complement(11700..13382)
                     /locus_tag="IVW84_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7917741.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(13393..13716)
                     /locus_tag="IVW84_001546"
     CDS             complement(13393..13716)
                     /locus_tag="IVW84_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7917742.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(13739..15145)
                     /gene="lacG"
                     /locus_tag="IVW84_001547"
     CDS             complement(13739..15145)
                     /gene="lacG"
                     /locus_tag="IVW84_001547"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7917743.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            15612..16619
                     /locus_tag="IVW84_001548"
     CDS             15612..16619
                     /locus_tag="IVW84_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917744.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17013..>17328)
                     /locus_tag="IVW84_001549"
     CDS             complement(17013..>17328)
                     /locus_tag="IVW84_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP7917745.1"
                     /translation="LTIPGAMSPTEIFKMFEMGADIVKVFPANCLGPEYFNQVQAPLG
                     SLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKSAVHQLNYERLMELAESFIDSLR
                     VE"
BASE COUNT         5200 a         3657 c         2845 g         5626 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
     8941 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
     9001 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
     9061 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
     9121 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
     9181 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
     9241 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
     9301 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
     9361 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
     9421 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
     9481 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
     9541 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
     9601 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
     9661 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
     9721 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
     9781 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
     9841 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
     9901 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
     9961 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    10021 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    10081 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    10141 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    10201 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    10261 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    10321 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    10381 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    10441 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    10501 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    10561 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    10621 atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc cctaaagtcc
    10681 attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcatt tagccctctt
    10741 tgcgaaggtg ttcctggatc tgcgaaaaag atggcaatat cgttcgagtt acttatagat
    10801 cgccagttgg aaaattcttt tccacaatca aatgtcatgg atttaaacaa gtgtttaggc
    10861 atggatttga accaatcatt taacctgttt tcgatatctt cggctcgtct gcctgtcggt
    10921 ttcaaagtaa taataacctt ggataaacac tcaaccaatg taataacagc gctcttgtgt
    10981 ttaactccca caatggtgtc gccttcgaga tgaccaaatt ctttagtcag gtgtggatag
    11041 agactgttcc gttcatgaat cgttctgcga aaggcctgtt ttccacgctt ttcttgatga
    11101 ttattcggtt tacgtttccc cttcataggt aacgactgtg tgtcaaaaag gcctttttta
    11161 aacatccgat agagtgtacg aacagaacaa tcaattggaa actcgccacg gccgataatc
    11221 acatcgggtg accacccttg agctaccttt tcttgaacat atgttgattg ttcatcagat
    11281 aaaacggttg aacgtcgtcc acaacgagat ttattttgtt tgtattgacg gtaataatct
    11341 agcacttttt tccttgtttg agaaacgaat aaacattata aatggtttgt ctagagcgat
    11401 ttaaactttg tgcgacaaga gcgacaggtt tctcgatatt gaaataagac tctatgatta
    11461 ctagttcgtc tgttgtaaga tgggtgtagg tcatttgtac tcactcctta ttaattcttt
    11521 ggtcggaact attttgagtg tagcacaaat gatcttttta gttgtctagc ttaattttac
    11581 aatcgacgcc tcaaaaagaa tacccgaaat ttaaacagca aaaaaagata gcgcttacca
    11641 ttgtgtttaa gataaaacgc tagctaagtt ggctagcgct ttattaatct aagcaagctc
    11701 taaaattgct tcaatacaaa atcaactgct cctttaggat cacgtgttaa attgatatat
    11761 tctgcacctt tagttgcaat tacttttatc cctaatttgt ctgcatcttt acggagatca
    11821 tccaaatagg aattcacttg aggtgctaga acgattaaat caaaattttt catcatatca
    11881 taatgagaac cataagctcc tgctttagca gaaagattaa cacctgtctc ttttgcgccc
    11941 tcttctaatg cattagccaa cattgcactc gttccggcac cagcacaaag tactaaaacg
    12001 cgctttcctt caccaaccga tgcttttgct tcatcaacta taactgccgc agttgcacca
    12061 acttcttgga tttcttcgac caattcttct tttacttctt ctgcagcaat gttttttaac
    12121 tcttgttcta ctaattcaga atcataggcc ttaacaaaag gtaaataaat aatcgcatcg
    12181 acaacaatca atactaccgc taatacaatc gccaacacac taaacccggt tcctaaaacc
    12241 aagccaatcg gtgcgggcgt cgcccaaggc aatacggata taaagctatt catccccaag
    12301 acatcgacaa aaagtttgaa tatccatacg ttagcgattg gtgctaataa aaatggaatt
    12361 agaaagtacg gattcaaaat aataggagta gcaaatagta acggttcatt taccgcaaac
    12421 gaaactggaa taaatgaagc tttaccaact gctttcaatt gttttgattt agcgaacaac
    12481 atgaacagaa aaggaacaac tagtgtcgct ccagtaccac ccatcgttcc aacaaagttt
    12541 cctagaccaa ctgttaatac ttttgaggct tgtgcaccgt ttttaaataa ttcaaagtta
    12601 gtttctacgt tcgcataaat aattgcagca atcgctggtt caacaatcga tggtccatga
    12661 acaccaacaa accagaacat tgccatggct ccccaaatga ttgcaatacc gacatatcca
    12721 tcagcagcac taaataatgg ctgcaataat tgaattacac ctgctgcaaa tgatgttcca
    12781 aaaaattgac gaactcccgt atcaatcaaa acggcaataa atactgcaat tgaaaagggg
    12841 ataatatctc taaacatctg tgtgattgtt ccgggaactt cttttggcat acggatcgtc
    12901 aaatctcttt taacacagaa tttatacaca tttacagtaa caaacgcaga aacgaaagct
    12961 gctaataatc cctttgtccc taaataatca gtagctatcc caccatcaat ttgatcagct
    13021 gccatcatta caaaaccaca aattgacgct aacataacag aaacttcatt aatctgctta
    13081 cctgaatcag ataattgacg gttcattgaa ttagctaaac atcgagctgt tgtagcaaca
    13141 accaaaagac caactaatcc cattgaataa ccataaactt tccaaagcca cgtcgagaat
    13201 gcctctggta acgtcacgtt aaatacttct ggaacagccg caatcataat aaaaatactt
    13261 gaaaatagaa tagctggcat agcagttaaa aagccatcgc gaattgcccc taaataaata
    13321 ttacgtgaga ttttttcaaa aaacggtttt cccttctcaa tttgcttaat aatcgtattc
    13381 atttttcttc tcctatgctt tagcaaattt ttctttgtac aattcaacaa aatcattgat
    13441 aagttctctt aatagaatcg ttgtcattaa atggtcttgc ccatgaataa aaattacgcc
    13501 tacttccatt tcgttccctg acgcttcttc agctaaacat ttcgtttgtg agttatgcgc
    13561 taaggttaaa ttttcatcag catccgctaa taatcgttca acattatcaa aattaccttc
    13621 tctcacttcc ttcaacactg ttaataatga acttctggca tctcctgcat aagcaacaat
    13681 ttcaaatcct aataattgca attctttcct atccatttcc ttgccctcct aactattttt
    13741 atattatcgt ttttgtttcg ctaacaaatt tataccaata tgcagactct ttcggatatc
    13801 ttttttgtgt atcgaaatct acatagaata gtccataacg cttagtgtat ccattaaccc
    13861 aagagaaaac atccattaat gaccaaatga aatacccctt tacattaact cccattttga
    13921 tagcatcgga aatcgcatta aaatgctggc gtaaataatc gattcgatca tcatctagaa
    13981 taatcccatc ttcaaaatta tccttatacc ctagcccgtt ttcagtaatg tatattttat
    14041 tataatttgg atattcgtct tggattcgta caagtaaatc atataaccct tttggataaa
    14101 tgatccaatc ccaatcggtt ttcggaagat cctcacgcac gattctctcg ccaatccctt
    14161 taatgcaata gacttggcta cctttttctc cggtagtgtt gtagtgaagt gtgctttctc
    14221 ctttgtactc tttaacccaa tggctaccat agttattgat accaagaaaa tcatttcgat
    14281 ttgctgcttt tttcatctct ctaaaatcat tagaatcaat atctgcagat tgcccatttg
    14341 ctgctaaaat ctcattaata tgtgccattg tttcatctga gtaataccct aaataagtag
    14401 catctaataa gaacttaatt gataacgcat catctaattt cgcagcatgc ttatcttctt
    14461 ctttgtcact tgctggatat tttgtctcta gcgagtgaac aacaccaatt tcccctttat
    14521 atccaccatc tttaaaaatg tttactgtct ttgcgtgcgc atacatcatg ttgtgtaaac
    14581 aagttacagt cttctctata tcaaattgaa cttctggagg aaacgatccg atcaaatatt
    14641 gatttgaagc aactggataa atctcattaa atgtactcca ataatgaacc tcagaaaatt
    14701 catcgaaaca gaactgtgca tagtttacga aatgttcaac attttctcga tttaaaaaat
    14761 ctccattatc aaatagttct ttaggggtat caaaatgatg aagagttaca aaaggaataa
    14821 taccatgctt attcaattca gcaaatacct tatgataata ttccactcct gtttggttca
    14881 cttccccata gccttttgga aaaatacggc tccatgcaat agacatccta atactatcaa
    14941 ttttaaattt tttgcataat tcagcatcaa ctggatattg attataaaaa tcactagctg
    15001 gatcagcagt atagcgtccc ttttctacaa ggaaagtatc ccacgctact tgcccttttc
    15061 caccttcttt tgtagagcct tctgcttgat aagctgccgt agctcctccc ataataaagt
    15121 cttctggtaa agttttataa ttcattttaa tctctccttg aaaattagtt tgatcaatat
    15181 gccaataatc cataaatcac ctcagcataa ttttaacatg tataagtaaa tttttttaca
    15241 caacgaaaaa ccgacctcga atatctattt tattgcgata tacaagcact tatatccctt
    15301 gatgtaaaat aaatatacca ttcaaaaacc gttttgtaaa cgtttttttg taaaataaat
    15361 ttatcttttt actaatttaa tttacacgaa acaagaaaac ggactgattc ccactaaaat
    15421 caatcccacc tactaatcat tctccaagtg gctatccatt atcgattcaa tcttctctgt
    15481 taatggatta ggctctctgc tgatatcaga gtttcgtttc ctgctagaaa aagtaattta
    15541 agttactaaa agagcagttt cgatttactt taacaaagaa aaggattatt atatccctag
    15601 gaggatgaac aatggttact attagagata ttgcaaaaaa atctggttac tcaacggcta
    15661 ctatatcaag actttttaaa ggtgatgagt cactctctat tacatctgat actaaaaaca
    15721 aaataatcgt tacagccaaa tctttaggat atgatctgac aaaaattaag actactctgt
    15781 acaagattgc cattcttttt tggatctcag aggaacagtt aaaaaaggat gtctactatc
    15841 aacatgtcaa agatgatttg aaaaattata gtaaaaaagc taatctagat ttgattttca
    15901 tctctaaaaa cgatggtgta aaaagcattc ctgacgatat tactggattt atcgcaatag
    15961 gacaattgac aaaaaaggaa ttgtactact taaatcaaaa agggtataag ggggtaatcc
    16021 gaggaataaa cccattacct gaacacttcg atactgtaga acctgatttc cacgaaatgg
    16081 ccagacaagc aattaactac tttattaatg aaggtttcac aaaaattgga tttattggag
    16141 gaaatttctt taacctagaa acgggtaaag aagagattga tagccgtgaa tatgccttcc
    16201 gatatcattt agaaaagagc ggactactca atgaaaagta tattttctcc caaggatcat
    16261 ttactgttga cgaagggtat caattaactc ttaaaatgaa agctgagtta aatgaagata
    16321 tgcccgaggc atgtttcata ccatctgaca ctatagctgc cggcgttctt cagggctgcc
    16381 atgaattggg tattaacatc cctaaagata tggcagtaat aagtattaat gatgacgaca
    16441 tcgcaaaatt cgttgcacca ccacttacaa cttttaagat agatatcgaa gagatagcta
    16501 aaacctgtgt agatttatta agtgatcaac tggtatattc gcgggacata tcaaagaaaa
    16561 cattaatcgg cgcaaaattg accgttagga aaagttttat ccctaaagaa gaggtgtaaa
    16621 ataagtaatt gaaacttatc tttaaaacta taattgtgtc cacatagtag ccgagcaatc
    16681 gggtttatgc ctattatgtt ttccataaat aatttgatgg tgatattttg acgtaaatac
    16741 aatatgattt tttcatcgcc aaattgtgcg cgctaatttt tgtttatcat tcagaacatt
    16801 tcctcctttt tatctaagta tgaggttgga taaacgcttc atcttagtat cttgggggat
    16861 tttttatctc gttgggaata tatcaagcat cagagcgcgc aaaaagaact ggtcgaaaaa
    16921 tcaatctacc agttcaatct cattcactgt gatactatgt tttatacaga taacgccata
    16981 ttataaaaca agttgtattc cgcaaatggg ttctattcaa ctcttaaact atctataaaa
    17041 gattcagcca actccattag cctctcgtaa tttagttggt gaacagcact tttttcaaaa
    17101 aattttgaac caattcctac ataagaggct ccattgttca aatagttttg tgcattagtc
    17161 tgatctaccc ctcctacagc catcaaaggt aaacttccta aaggggcttg cacttgattg
    17221 aaatattccg gtccaagaca gttggcgggg aacaccttaa caatatcagc acccatctca
    17281 aacattttga atatctctgt gggcgacatg gccccaggaa ttgttaga
//
LOCUS       DACCWV010000056        16855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCWV010000056 DACCWV010000000
VERSION     DACCWV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.056"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..177
                     /locus_tag="IVW84_001550"
     CDS             <1..177
                     /locus_tag="IVW84_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7917746.1"
                     /translation="ILTSLTIILTIPTIVGSIYGMNIKLPIAERDDAFIWLVLLTLFI
                     CIVTTYYLKKGKFL"
     gene            250..366
                     /locus_tag="IVW84_001551"
     CDS             250..366
                     /locus_tag="IVW84_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7917747.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(421..2103)
                     /gene="recN"
                     /locus_tag="IVW84_001552"
     CDS             complement(421..2103)
                     /gene="recN"
                     /locus_tag="IVW84_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7917748.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2121..2570)
                     /locus_tag="IVW84_001553"
     CDS             complement(2121..2570)
                     /locus_tag="IVW84_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7917749.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2677..3495)
                     /locus_tag="IVW84_001554"
     CDS             complement(2677..3495)
                     /locus_tag="IVW84_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7917750.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3504..4394)
                     /locus_tag="IVW84_001555"
     CDS             complement(3504..4394)
                     /locus_tag="IVW84_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7917751.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4396..4620)
                     /locus_tag="IVW84_001556"
     CDS             complement(4396..4620)
                     /locus_tag="IVW84_001556"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7917752.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4625..5962)
                     /locus_tag="IVW84_001557"
     CDS             complement(4625..5962)
                     /locus_tag="IVW84_001557"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7917753.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5963..6817)
                     /locus_tag="IVW84_001558"
     CDS             complement(5963..6817)
                     /locus_tag="IVW84_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7917754.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6914..7363)
                     /gene="nusB"
                     /locus_tag="IVW84_001559"
     CDS             complement(6914..7363)
                     /gene="nusB"
                     /locus_tag="IVW84_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7917755.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7356..7781)
                     /locus_tag="IVW84_001560"
     CDS             complement(7356..7781)
                     /locus_tag="IVW84_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7917756.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8027..9091)
                     /locus_tag="IVW84_001561"
     CDS             complement(8027..9091)
                     /locus_tag="IVW84_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7917757.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9263..9508)
                     /locus_tag="IVW84_001562"
     CDS             complement(9263..9508)
                     /locus_tag="IVW84_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7917758.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9647..10021)
                     /locus_tag="IVW84_001563"
     CDS             complement(9647..10021)
                     /locus_tag="IVW84_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7917759.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10104..11018)
                     /locus_tag="IVW84_001564"
     CDS             complement(10104..11018)
                     /locus_tag="IVW84_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7917760.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11039..11842)
                     /locus_tag="IVW84_001565"
     CDS             complement(11039..11842)
                     /locus_tag="IVW84_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7917761.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11875..12864)
                     /gene="manX"
                     /locus_tag="IVW84_001566"
     CDS             complement(11875..12864)
                     /gene="manX"
                     /locus_tag="IVW84_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7917762.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12967..13458)
                     /locus_tag="IVW84_001567"
     CDS             complement(12967..13458)
                     /locus_tag="IVW84_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7917763.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13649..16483)
                     /locus_tag="IVW84_001568"
     CDS             complement(13649..16483)
                     /locus_tag="IVW84_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7917764.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         4732 a         3754 c         2798 g         5571 t
ORIGIN      
        1 attttaactt ctttaacgat tattttaact ataccgacaa tcgtcggcag tatctatgga
       61 atgaacatta agttacctat tgctgaaagg gatgatgcct ttatatggct agttctatta
      121 acattattta tttgtatcgt aactacctat tatttaaaaa aagggaagtt tttataaaaa
      181 agccctcttg tggaaatgga aaaaagattg tacacttgaa tcaactaatt aatgacgagg
      241 ttgaaaacaa tgcgtgaaaa aaaacaaaca agtacttttg ctaaagtaac aaaatttgtc
      301 atttggacaa tgctgatttt aacgattgga tctgttgtat tgactgctgt aatgagtgtc
      361 atgtaaaaat aaaatatagc tatcagctgc tgaaaaggac ctgaaccact gcgaggtggc
      421 tcaggtcctt tttgcgagtt tcaatagttc ttttgcatgt tcgagtgtga gttttgtaat
      481 ctcacttcca gccagcatac gtgcaatttc ttcttctctt tcttttgttt tcaactctga
      541 aacagaagtt tgcgtacgtc catcaatcac tgctttcaca atataatatt gatagtcggc
      601 gactgcagca acttgaggca gatgagtgat gcacaaaact tgagagtatt tcgatatttg
      661 agaaatttta tctgcgatgg cctgtgcaac tcttccgctc acacctgtat ccacttcatc
      721 aaaaataata cttgtcacac cttgctcaga agaaaaaata gttttcaaag caagcaacat
      781 ccgagacagt tcgccaccag aagcgacttt caccaaaggc ttcagtggct ctcctggatt
      841 ggtcgtaatg taaaattcga tttggtcaaa tccatcatga ttcaattgtc tattttcttc
      901 ttttaagaaa cgtacctcaa actcagtgtt ttccatatac agacttttca attcatgcag
      961 aatggatttt tccaattctt ttgcgatttt tttacgcgct tcatgaagat ctgcggcttg
     1021 ttgctgaagt agaagctctt tttgctctag ttcctctttc attttgtcca attgaccttc
     1081 tgaaaaatca gaagaagcta gctcttcggt gatttcgtca tagtaagcca atattgactc
     1141 aatggattcg ccgtatttac gtttcaactg tcggattaat tctaaacgtt gcgtaacttc
     1201 ttctaaacga ctctcatcta actccaataa atcaatctga cgactcatat cgccgattgc
     1261 atcttgcagc aaatagtaag cactttggat attttcataa attgcttcat actccacatc
     1321 tagatgggcg atcccttgca gttctgaaac ggccaagcca acaccatcta cactgttttg
     1381 ttcattattc gtcagtgctc cgtaacctgc agctaatcca tcagcaattt tttgataatt
     1441 gctaagtttt tctctttctt cttttaattt ctcttcttct ccgacttgca aatcagcagc
     1501 agctatttct tcttgctgga aatggagcat atctattctc tgaacatagg atttttcatt
     1561 ttgctgcact tttcgtaccc ttttttccag ctcacgataa gagatatatt cttgctgata
     1621 actctttttc ttctgttgga aagctacatc tccaaaacga tccaataaag caagatgtga
     1681 ttcaggctgg agcagttcct gatgttcgtt ttgtccttgg atatctacta aataactgcc
     1741 gattcttctc agattagcta atgtgataat atgaccattc actcgacaaa cattttttcc
     1801 agttaaggac atatcccgtc gaacaatcag attgtcctca tctgtttcga tccctaattc
     1861 aaccatcagt tcagagaatc cttcttgttt tggtagttca aacaaccctt caagcacaca
     1921 tttttctgct ccttgacgta tataatcgct tgaacctctt cctcctgcta acagacctaa
     1981 agcatcgata ataatggatt tacccgcacc agtctctccg gtcagcgcgg tcattccttc
     2041 gtgaaaagaa agccgcaatt ccggaataat cgcaaagtta gtaatactga tttcttgtag
     2101 catcacattc gctcctcaac ttaaaggtaa tgcaggaagt ctttcttcaa taaaactgcc
     2161 tggtcttcag ttcgtgtaat catcaaaaca ttatcgtcat cattgataat cgaaaataat
     2221 tctttcttgt aacgtttatc aatcaaattt gctgctgccg aagcatttcc cggcaatgtt
     2281 ttcaaaataa caaatttttc catttggtca actgccacga aagcgtcttt taatagtttt
     2341 tccaatttag tatttacatc ttcgtttgtt tcagctggta tgctataacg gtagccgcca
     2401 tctgaagccg gtaccttgat cagtttcagt tcctttatat ctctggaaat cgtagcttga
     2461 gtaacagata ttcctttgct tttcaggatt tcaacaaatt cttcctgctt tcggatatct
     2521 tgctcactca acaatcgagt aatcaaacga tgccgatctt tttttctcat tacgtcttcg
     2581 ctccttagca tattcttctt aatcaatgat agcttatttc cttggtataa taaagaagaa
     2641 tccgcatact tctcacttga ttttcacagt tacaatttat ttatggtcaa actgttcgtg
     2701 tgcatttgaa accacttctg ccatattaat agctgaattg attgttccgc tttcgggtac
     2761 tttcctcaag tgagccaaga actcgatatt tccttctccc cccgtgatcg gagaataact
     2821 taattccagc acatcatatc caccttgtgc cgcaaatgac aaaatgtctt ctaaaacttt
     2881 ttgatgggtc tgagggtcac gaacaattcc gtttttccct acagcttctc tgcctgcttc
     2941 gaattggggt ttgattaaag caatcacttc tccctcttct tttaaaatag catgtaatgg
     3001 aggcagcatc agttttagtg aaataaaaga aacatcgata acagcaactt ctggaattcc
     3061 taaggtaaag tcttcgggtt ttgcataacg aaaattcact ctttccatta cgacgactct
     3121 aggatcttgt cttattttcc aagctagctg attgtagcca acgtctaaag cataactcat
     3181 ttttgcgcca ttttgcaaag ctgcatcagt aaatccgcca gtggatgcac caatatctaa
     3241 caatgttttt ccttcaacag atagatcaaa tgctttgagt gctttttcta attttaatcc
     3301 ccctcgagaa acataaggaa gttttttacc tttgactctt aattcactac tcactggaat
     3361 tttttctccc ggtttatcaa accgctcgtt tttttcgttg taaatcaaac cagccatcac
     3421 tgaacgtttg gcttgttctc ttgtttcaaa taatccttgt ttgaccgcta atacatctac
     3481 gcgttctttt tccattcttt ccctcatttc tccacatcaa attgcttgat catctggcgc
     3541 atcagcccgc catcaaaaga aaaaacgtcc tcttctaatt tgtctatgat cttgttggcg
     3601 gataacagct caatctccaa cgcctcacgt gtcttttcta ggcctaataa acgcgggtaa
     3661 gtattttttt ccatacgttc atctttacca gctgttttcc ctaatgtttt cgttgtacca
     3721 atcacatcga gtagatcatc tcttatctga aaagctaaac ctaaatgttc ggctaatcgt
     3781 tgaagtagca ataagatttc ttcaggttgc ttcgctaata tgccaccagc cagcaaggca
     3841 taacgaatca aatgccctgt ctttctttca tgaataaatg ctaattcttc taaagaaagc
     3901 tttttcgatt cgccctcgat atctgcagct tgcccagcaa ccataccttg tgatcccgca
     3961 caaacagcta attgctgaag cagcagaagc ttaggagaat tacctaagtg tgccatactg
     4021 attagctgga atgctcctgt aagcaaacca tctccggcca atatagctaa tgcttcgccg
     4081 aatactttat gattggtagg ttttcctcta cgaagatcat cattgtccat cgcaggcaaa
     4141 tcatcgtgga tcaaggaata cgtatgcacc atttctaaag cagctgctac ttgatagacg
     4201 ggaacatcaa taggctcatc aaaggaagca acggccgtta aaagtaataa gggacgtatt
     4261 cttttcccgc ctgctttcac tgaataactc atcgcatcat agagtcctct atccgttgta
     4321 tgctcatcta aaaaagacaa gatttctttt tctacagcag gtagatgaat agctgaaaag
     4381 tcagttaatc tcatctcagc tttcctcgtt tccgtcaaaa tcaacttctt catttgattc
     4441 tgtcatcatt ttcgtcaagg tctcttctgc tttagctaat gtatcttggc aatgtttgct
     4501 taattccatt cctcgtttaa acgaatctag agctgattct aatggaacgt ctccttgttc
     4561 taactgcatg acaatttttt ctaattcttg cagtgactct tcaaaagttg gattttccat
     4621 atttttattc tcctttgtct atctcttgta cttgtccttt aacgattcca tcttgataat
     4681 ggatcgtcag caaatcccct tgatgaatct cggtgacact ttttactact tgatcattta
     4741 acgtcgtata gctgtagcct cttcccatga ttttcaatgg actcaacaaa tcaagagatt
     4801 gaacagcttg agcaaaacgc tgctgctgtt gtttcatata ttgttggatt ttctcttcta
     4861 agcgtttttc tagataagca gtttcttggc gagctgcttt aactcgataa agcggagcca
     4921 cttgttccaa ttggtggctt aatgcgatcg tttgtttttc tttcgcatga taaatttgct
     4981 gtgtagcttg atataatcgc tgtttcaact ggtctaattt gatggtctga ccttcataca
     5041 atctttcagg ctgccgaaat acataggaac gtcgagcacg ctcgaaccgt tcttgtttgc
     5101 gctggatctg gcgtacaaac gcctgctgca atctagcttg ccgttcttga atgcgcatca
     5161 actcttctga caaaacagga acagctaatt ctgcagctgc tgttggagtg gcagcccgaa
     5221 catctgctac taaatcagcg atcgtggtat ctgtttcatg tcctacagat gaaatcaccg
     5281 gcgtattagc ttgataaatc gcccgagcaa cccgctcttc attaaacggc cataaatcct
     5341 caatcgaacc acccccacga ccgatgatca ttgtatcgaa attgcccatc tcttctactc
     5401 tctggatatt cctgacaaca ttgtccgctg cttggtttcc ttgtacaact gttgggaaca
     5461 aaaccaattg gacaattgga tatcttcttt tcaccgtcgt gatgatatcg cggataaccg
     5521 ctccgcttgg actggtaagg accgcgattc gctttgggaa acgtggcagc atttttttag
     5581 gcccttgaaa aaggccttcc ttcatcagct tttcttttcg ttcttccaat tcttgatata
     5641 aagcacctat tccatcaggc tccatgtgct ctacatagat ttgataagaa cctcccgctt
     5701 catataatga aatacgccca acgacaagga ctttcattcc ttcttttggc tggaatcgta
     5761 atttttggaa tgctcctttg aacataatag cagatatttt tgcatggtca tcttttaagc
     5821 tgaaatattg atgggcattg gcacgtaaac ggaaattcga tatttcgcct gttaaatata
     5881 cgcgttctaa ataaggatcg gcatcaaatt ttcttttcaa atacttcgtt aatgtagtga
     5941 cagttaaata ttcttgtgcc atctattcac ctgctccaaa agcttcggca gattttacgg
     6001 tttgatacat cagcatcgtg atagtcattg gccctacacc ctttggcaca ggagtgatcc
     6061 agcctgcaat aggctccact tcatcaaatt tcacatcacc gatcagctta ccgttagcat
     6121 cacgattcat ccctacatct atcactacag cacctggttt tacgaattct tttgtgacaa
     6181 aatgtccacg tcctattgct acgactaaaa tatctgcttc ttgtgccagt tcagccagat
     6241 tttcggtttt agaatgagca acagtaacag ttgcatctgc catcaagagt agctgagcca
     6301 tcggtttacc gacgatattg cttcgtccga taactactgc tcgtttaccc gccggatcga
     6361 tagaataagc ttcaaacatt ttcatgatgc cgtatggagt acaaggaatc ttgtccggtt
     6421 tacccgcaag gagtctgcct aaattcatcg gatgaaaacc gtctacgtct ttttcagggc
     6481 ggatagctaa caggatcttt tcttcatcta tatgctttgg caatggtaat tggaccaata
     6541 taccatgaaa actaggatct tgattgtatt tttcaatttc tgcaagtagt tcgtcttcag
     6601 aaatcgtctc aggataacgc tctaccaatg aatggattcc gatttctttt gctacacgtt
     6661 ctttatttct tacatagatt tggctggctg gattttcccc taccaataaa acgactaatc
     6721 cgggatggat accattatct ttcaattttt ggattttttc agcaatctct gcctgcattt
     6781 tttcagctaa ttcttttccg ttaatcaatt ctgccatcta accactccca gatatttttt
     6841 gtgttttcat tctaccatag aattttcccg atttataaca ggtttatgat gattcataga
     6901 aaaaagctag gacttacgcc ccagcttcta attctttcaa tacattggat aaaacaccgt
     6961 tgacgaattt acgtgaacgg tcatcactaa atgtttttgc caactcgatt gcttcattta
     7021 aagcaacagc cgctggtaca tctgtcacat aagtcatttc aaaaatcgcg atacgcaaga
     7081 tcaccaaatc catttttgag atccgattga tgtgccaatt gttttttaaa tgtttttcaa
     7141 tcactttatc caattcatct ttttttgcac agacaccgcc aactagcgta tcaagataaa
     7201 caggaacaaa ctctgattca tcttcgttga tcatgtcgcg gtggtctaat tcgattgcgt
     7261 tgaaaatcgc atcttctttt gttaggtctg cattgaagtc taatggaaat aaagcctgca
     7321 atgccatttc ccgaatctcg tgtcttgata attctttatt cattctcttc ctcttcgtct
     7381 tcaaaaagtt cttcgattgt tggttgttct aatttttcag gaacaacagc tactacatgg
     7441 atattgactt cagataaatc gatatccgtc atgaaaagga cttgctggct gacccgttcc
     7501 tgcatttcca ttgctacttt aggaactgaa acaccatatt ctagataaca gtaaagatct
     7561 actttcaagc catcttcctc gtttgctaag taaacacctt ttccgtgagc tgcacgtcct
     7621 aataattcag tgacattgct ggcaaaagtt ccgcgcattc cgtatacgcc ttctacttta
     7681 gaagctgcaa tcccgataat tacttcgatg acttcaggag cgatcacgat ctctcctaaa
     7741 tcttggtttg cgtttaaaat caaatttttt tcatctgcca tttatttatt cctccctttg
     7801 cctaagcacg agatacatcc gaattctcta tgatgcggca tagtgatcac gtggtaagaa
     7861 aactgccgcc gatcagctgc aaactgctat gaattggaga aaacacccaa ctttattatc
     7921 tcttacagct taaaagttct tctatagtgt atcattaaat cgagtgattt tctataacaa
     7981 agtctgtgaa cactgatttt tacgcttact tcttctattg atgaaactat aagatgatca
     8041 attcttttgg tgaacgagtc aacacacgat ttccatcttc tgtgatcaac aagtcatcct
     8101 caatacgtac tccaccgatt cctggtaagt agatccctgg ttcatcagtg atcacatttc
     8161 ctggtacaaa ttgcttttct gcacgagaag aaacgtttgg tccttcgtgg atctctaaac
     8221 cgatcccatg tcctgtagaa tgtccaaatg cttccccgta accatgtttt gaaatataat
     8281 ctctagcaac tgcatctaat tggacaccag tcattcctgg ttttgcagca tcaataactg
     8341 ctaattgagc atctaagact atttgataaa tttcttttaa tttttcccca ggatcaccaa
     8401 tagcgaatgt acgtgtcata tcggaaacgt acccttcgta atagcaacca aagtccaatg
     8461 tgatcaagtc accttgttcg atgatttttt tgcttgctac accatgcggc atagccgaac
     8521 gcaatccgct agcaacgatc gtatcaaaag aaacacctga agcgccaaga gaacgcatat
     8581 aaaagtctag ttgatttgcg acttcaatct cagtcatgcc cggttggatc attttcagga
     8641 tgtgatcata tgccatatcc gcaatatgac atgctttttc gataatagcg atttcttctt
     8701 catctttcac ttctctgagt tcttcgatca tctgtgaaac aggtactaat tccgcatcga
     8761 tgatttcttc taaaaccgaa tattctaaaa atgagactgt tgtttcctca aaagctaatg
     8821 agtgcagttc ttctttgcgt actaagtttg ctacctcatc aaaaataggt ccgacatttt
     8881 taataatttc aaacccttga gcctgcgctg ccgcctgctc ggtataacga aaatcagtaa
     8941 taaaaaatgc tttttccaaa gtaataacag ctaatcctgt cgtacctgta aagttcgtta
     9001 agtaacgaag attgtacgga ctggtaacca aaaatgaatc caaattttct tcttgcattt
     9061 ttttgcgcaa tttttctaca cgtagcatca taatgaaact ccctctttct ttgcacgctt
     9121 ttttccatta tagcaaactt ttcttcgtac acgacagtta aataaaaatt tccggaaaac
     9181 ttctttttat tttaaataaa taaagccaaa aaggagtcac agtctcgctg caactccttt
     9241 tagtttacta ttttgtaaaa acttatgatc gttttccaaa gaaaaatgag acaactgcta
     9301 ccaaaatcac tgcgcctaaa atagatggaa taagtgccat acctgcagct tttggtcccc
     9361 attcgccgaa aatcgcttgt ccaatacttg aaccgactag tccagcaatg atattcgtta
     9421 cacagcccat cgaatttcct ttactggtga tcgcacctgc aattgctccg atcacggctc
     9481 caacaatcaa tgtccataac caactcataa tataacctcc ttctttgcta atcaatttta
     9541 gcatatggga tttaaagggg acaaataatt ggttttgcta tttcatattt tcttctgcaa
     9601 agtaaaaaaa taaagaaaac ctcctcttag aagttttctt tattttttac ttgcgtttaa
     9661 acatactacg aataccacgt ttcactacat caaagaaact caatgattgt accattctat
     9721 cttcatctac atattctctg atcgcacgaa gcactttttt aggttctttg gaagaaaatg
     9781 tgtatgtacc attttttttc gtttgaatgg catatcgagg aatccatttt ccctttagca
     9841 tgactgatgc tagtacataa tccacttctt cccatggaat ttggatatat ttgcgtctat
     9901 ctcgcgcatg gtaaaactca aaaccattat tacctatcat gatttgcccg taatctgtca
     9961 gacccgtaaa cgaagtagca tcaattacca agtctacttt tgtattgatt gattgaacca
    10021 ttttctccct ccagatattt gtttatgaac agtatactat ataaaaaaag ctcgggacaa
    10081 cgcccgagct atatttttat ggattataaa agtccgatga cgtgtccaac aacaccgact
    10141 acgaataaac caaggatgat aacgattgga gaaacctttt tcttaagcaa ccacatacat
    10201 aacaatgtta atcctaatgc agctaaacca ggaatcaatt ggtctaagtt gtcttgtaat
    10261 gttgttacct tttcaggaga aagggctaag ccattattaa cttgttcaaa tgcactcttg
    10321 attccttcac cacctgaagg caatttatcc cattcgatgt aagcaccttt atctaatttt
    10381 acagttgata caactggtaa gaatttgatt gatacccatc tttgtacaag ggcagctaat
    10441 acgaacatcc ctaaaattga agcacctttt gttacgtctt gtaacaatcc gccagaaagg
    10501 tcatcagtaa ttttagaacc agctttgtag ccaaattctt gtgtatacca catgaatgcc
    10561 caacgaataa tgttccaacc taagaagaag atgattggtc caaggatatt tcctcccata
    10621 gctaatgaag cacctagtgc acctagcatt ggacgtactg taaaccagaa cactgggtca
    10681 ccaacaccag ctaaaggacc catcatacct actttaaccc cttgaatcgc tacatcatca
    10741 acaggcgcgc cattcgcacg ttcttcttcc agagccaatg taacccctaa aattggagaa
    10801 gcaatgtatg ggtgtgtatt aaagaattcc aagtgacgtt ttaatgctgc agaacgttct
    10861 tcttttgttt tgtataattt tttgattgct ggaatcatag aaaatgccca gccaccgttt
    10921 tgcatacgtt cgtagttcca tgaaccttga atgaaggtag agcgccacgc aacagctaaa
    10981 cgatcttttt ttgataattt gattttttct tctgccattt tattttcctc cttcaagatt
    11041 aatagtcatt caaaatgtcg cctaaagggt caccagagtt gcttccgcct ccattatttg
    11101 atgaaccacc cattttagaa aggttcaagt aaataagagc taatgcaaca cctaaagcac
    11161 ctagagcaat caatgttaat tgagaaattg cagctacaac gaaaccaata acgaagaatg
    11221 gccatacttc ttttgtagcc atcatgttga ttaccaaagc gtaacctaca gctacgacca
    11281 ttcccccacc gatagccata ccatctgtta accatgctgg cattgattca aggaatgatt
    11341 gcacagtttg tgctgggata aatagcaacg ctgctgctgg gaccgcgata cgaacccctt
    11401 gtaaacatac agcaatgatg tgccacattt caaccttacg gatgtttcct tcttcagcag
    11461 ccgcatccat caaatgaacg attggaacag cgattgtacg tacgatcatt gttaagaaca
    11521 accctgcaac agccaaagga acggcaatcg cgatcgcaga aggaacacct ttaacacctt
    11581 gtccacctaa aactaaaata attgctgatg ctactgatgc taatgcagca tctggtgcta
    11641 cagcggcacc gatatttgcc caacctaaag cgatcatctg aagcgtaccg ccaagtacga
    11701 taccagcttc gaggttaccg gtaactaaac cgatcaatgt acatgccact agtggttgat
    11761 gaaattgaaa ttcatcaaga ataccttcca taccagctag aaaggcaact aatacgacta
    11821 aaattattga aataatagac atgataagcc tcctataaat tttaatatta tttattacgc
    11881 ttttgctaat tcagcttttg cttttttcaa aatatcatcc atgttatcgc gtgaatcatt
    11941 aggtacttta cgtacatcga atttcacacc tttttgttca agcttctcaa acgcttcaac
    12001 gtcttcttgt cccatagaca atactttact tacaacgact ttaccgaccg aatgcgccat
    12061 tgacccaaca tttaattctt tgatgtctac gccaccttca acagctgtca atacgtcttc
    12121 aggattttca aataaaagca atgctttggt atttccaaaa cgagggtctt ttgctacttc
    12181 gatcattttg ctaataggaa taacatttgc ttttacacct ggaggagcag cttgttcgat
    12241 tagtctctta cgcagatcat cctttgatac tgcatctgaa acaacgataa tacggtttgg
    12301 ttgcactgct tttgtccaag ctgtcgcaac ttgtccatgt aacagacgtg agtcaacacg
    12361 tgctaataca tatttgattt ttccgtctcc gaccacagtt ccttctggaa gtgctccttt
    12421 aggttgtgcg tcttcaactg ctgctttcgg tgcttctgcc ggttctaatt cttccggttt
    12481 tacttttaca ccgtcttttg ctgtttcaat gatgtgtgct gcaatttcat gagcagattc
    12541 cattgaaaag cgtgatgcgt aggcttcaat caacattggt aaattcaaac cgctgacgat
    12601 tgcccattta tctttgtgct cttcgaataa tgtgtttgat tgattgaacg gtgttccgcc
    12661 ccataaatca actaagaata acacttcgtc ttgattatca aaagaagcaa ccgcttcttc
    12721 caatttggct ctcaagtcat ctggaccttc acttggtttc aaaatcacgg ctttgacgtt
    12781 ttcttgttcg ccaaagatca ttgaaccgga ttgtaagatt ccttcagcaa attgtccatg
    12841 acttgctagg ataatcccta ccatatttat acctcctatt tacttttgtt gtaacaaatt
    12901 caaaaatatt aataattgct aaccattaca acagaattat ttgacctttt ccaacaacca
    12961 ttcttgctac acctttttga ttttcgctag tagttccatc aaaaggattt tcctgtcagc
    13021 aggtactttt cgaatctcta actccacttc ttgactgttc aagtaacgaa aggaatcaat
    13081 atcttgttga tttactgaaa caaaattggt aatcattgat ttgccttcag taaaactcat
    13141 accgccgata ttcaacgact tcaactgtac accgcagcgg acaaccgctt caacatcttg
    13201 gggattggtg aataaaagca tcacttttgc tcgtgataat aatccactat gatagatttg
    13261 aatcattttc tgcacggtta ttacatttgc tgtgatcccc ggcggtgcag cttcttttag
    13321 tagaaacttt ctcaatttat cttgagcgac tacatcgctg atcacaatga tacgttcaac
    13381 acctgtttgc ttcgaccaaa ctgttgcgac ttgcccgtgg attaaacgat catctatacg
    13441 tgccaatcga atatccatct gcaaaactcc ttcttcctca ctacttcaca gaaaccccta
    13501 tcatttctgt gtgaacaacc acctcctagc acacactact ataagcaaaa agcgtgccaa
    13561 cttttttacg atacactaat tgaatttaaa ggattcttaa ccaatacaca aaaacgatac
    13621 actataaaag tgtatcgttt ttgtgtattc atggcacagt gtattctttt ttatctaact
    13681 gattatggac tagacgtagc agataataca tttcttctat cggcactttt acttttatct
    13741 gatcttccaa cagaacaact gaggctgcca attggctata aagaggatca ttcgttgtat
    13801 ttgtcaattc gttttcttcc acagtcaatg gttcattccg aataatacgt tcgatcattc
    13861 ctgctgtgtg catcactagg ttgattttga atccatattc ttcatcgccg attcccgtcg
    13921 tctgagagat ttgtgtagaa aattgccaca ttggatcgat taacttacta ccattaataa
    13981 agatgaagtt atcagagata aactcaacac acgtattttt agctgactct tcatctaggg
    14041 agatgaattc agtttcctcc aattctgatt ccatcagcaa ctgatccaaa atgacttcga
    14101 tattttgatc aataaaacgt tctaaaggaa taaatggggc ttggatattc aaatcagtaa
    14161 tcccagtagt agctatgatc tgatattctt tttggataac tggcagttct tctttcactt
    14221 ccacaatcga caaagctaga acttctaatt ctgtttcttg acgcttgttt accgctcgtt
    14281 cgatcaattc tttgattctt tgtgctgtac cttcgccaga tgcgcagata gccaaaacag
    14341 cttttttctt atgaataggt tttgcttcct gaacagattc tacagaagat atttctgcgt
    14401 aacctcgaaa atttttcaat gattcatgca acatatctag atctgtccct aatactgaga
    14461 cctttcgtac agtttccaaa actagagaag ttgttaccat atcaacagta cgtactggaa
    14521 ttccagtgtc tctttgaatt tgcgaattaa acgacgcaag ggaccccata tctactaaga
    14581 gtatgacccc gcttccttca tctacttctt gtacattgcg ttcaattcgg tccaatgctg
    14641 ttttcggatc catatccaaa ggcatgtcta ctgctcgtac gttctctgca tctaacaact
    14701 gttggacaac ttggaccata ctgcttgctg tgctgtttcc gtgagctgca acgactacac
    14761 cgattctgcc gcttgcttga tttgcccgta acgagacgag tagaacggtc aagtaatagt
    14821 cttcactttc tggaatctcc acttgaaaat acgtttcgat ataactacgc acttctttcg
    14881 caacagcata ttcatctgga aaatcaaaag ccatctcacg gatgttatca ttcgtatgcc
    14941 gttcttctcc taagttgatt tttttcaaaa aagaactgat atgcaagctc atagcataaa
    15001 taaagttttg ctgaaactca tatggtaaag atcttttgac cattacataa atctgattgg
    15061 tcacttctat gattttcgga tctacaaatt ctgctaattt gctgtctgca ttaaaagaaa
    15121 aaccatggtc tttatagaaa gacttcagat ggatgttgat atctgtagaa atgaaatgat
    15181 tgattgcttc ttgatccaaa ccatccgact tcagtaaagc tgctttatct ccgatgatat
    15241 catatagatt ataaggtaac tcataggagt ccgattggat tttcatgatc gtatcatttg
    15301 gtgctacagt tatctttggc tccaatattt tagaaagctc tgccattgca ctgcggttgc
    15361 ttgctaattg aatcaatccg ctacgaatcc cttctgtcaa atcatcgatt gtgatcgaga
    15421 tttcgggtga ttgcatatga tttagaaatc ctcgtgcaca tactaactgg atatttgact
    15481 tcaattgtcc tatatttcca taggtcacac tgccgatcaa tgcttttgct acatcttctg
    15541 tcaaagaaat ctttcgctgc gtgcggtttg cttcgtgggc aatcatgatt tttactaaat
    15601 cgactttctc atttgcagga cgtttttcaa aagccggaag tttgatattg attgggatac
    15661 gccgaacaaa agtttctaat agagaagaac ctgggtcttc tgtggtcgca cctacgatcc
    15721 gtacatttgc ttcatgggat ttcgttgttt ccccaagacg actgtaagtt ccatgatcca
    15781 taaagtaaaa gatcatttct tgaccttcag gcggcaaccg gtgaatctca tccaaaaaca
    15841 gcataccgcc atccgcttga tcgataatcc ccattttttc ttcatctgcg cctgtaaaag
    15901 caccttttgc gtacccaaat aaatgactca tcaaaagctc agggttattg gcataatcag
    15961 cacaattgaa aacaatcagt tccgtttcct catctattac atgatgcgct tttgcaaaat
    16021 gatacatcgc gtgtgcaaaa taagtctttc ctgatccggt aggtcctgta atcaaacaat
    16081 ttaatcctct tggcggatac aaaatcgcag cttttgcctg ttccactgaa tttttcatgc
    16141 tgccgttagc tccaatgatt tttgcaaaga tatccttagt atctatttgg agtgtatttt
    16201 tttcttcaga gaccggcgtt tctttgtaac tgactacatg ttttgtaact ggctttacgt
    16261 gcgtaacgag tgtttgatat atgtaacgta ctggtcgtcc gtctgtcttt atcagttttc
    16321 cttcgcgcac tagctgattc agatctttac tgctatttgt tcgctgaatc cctaacactt
    16381 cagcaatttc ctgtgttgtt actccttgat catattctgc ctttgttaaa tgagcggttt
    16441 tttctttgac atacatataa attcggtcaa tgcgttttgc caatggtatc gctccttttc
    16501 tatagactat tgtacttagg cttcaaaaca taatactatg tatttatttc tgtttcgtca
    16561 actatttacc tgattttgtt ttgttgataa cacgcggata gatagaaatg ttcaatttaa
    16621 aagaacgaaa aatcccgata cgatttttat aatggaaaca agaaaaagaa cactgtttac
    16681 catataatta tttcactgat agcaaacaat atagcttcca tcaattgaaa acgatagtta
    16741 tcaaaacatc ctgattccaa attggcaaaa aaatattaag tgtaaaaaga caaagtaaac
    16801 ttcgtagcct ccactacaac ttttattttt ataaacaaaa aaggctgtga caaaa
//
LOCUS       DACCWV010000057        16629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCWV010000057 DACCWV010000000
VERSION     DACCWV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.057"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            37..3717
                     /gene="nifJ"
                     /locus_tag="IVW84_001569"
     CDS             37..3717
                     /gene="nifJ"
                     /locus_tag="IVW84_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7917765.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            3906..4793
                     /locus_tag="IVW84_001570"
     CDS             3906..4793
                     /locus_tag="IVW84_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7917766.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4790..5959
                     /locus_tag="IVW84_001571"
     CDS             4790..5959
                     /locus_tag="IVW84_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917767.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFPPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            5983..7338
                     /locus_tag="IVW84_001572"
     CDS             5983..7338
                     /locus_tag="IVW84_001572"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7917768.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(7410..9215)
                     /gene="glmS"
                     /locus_tag="IVW84_001573"
     CDS             complement(7410..9215)
                     /gene="glmS"
                     /locus_tag="IVW84_001573"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7917769.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            9625..9915
                     /locus_tag="IVW84_001574"
     CDS             9625..9915
                     /locus_tag="IVW84_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917770.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9928..10953
                     /gene="sppA"
                     /locus_tag="IVW84_001575"
     CDS             9928..10953
                     /gene="sppA"
                     /locus_tag="IVW84_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7917771.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            10956..11615
                     /locus_tag="IVW84_001576"
     CDS             10956..11615
                     /locus_tag="IVW84_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7917772.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            11772..12704
                     /locus_tag="IVW84_001577"
     CDS             11772..12704
                     /locus_tag="IVW84_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917773.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            12858..14147
                     /locus_tag="IVW84_001578"
     CDS             12858..14147
                     /locus_tag="IVW84_001578"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7917774.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            14394..15644
                     /gene="clpX"
                     /locus_tag="IVW84_001579"
     CDS             14394..15644
                     /gene="clpX"
                     /locus_tag="IVW84_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7917775.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            15727..16314
                     /locus_tag="IVW84_001580"
     CDS             15727..16314
                     /locus_tag="IVW84_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7917776.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5457 a         2961 c         3660 g         4551 t
ORIGIN      
        1 gataataaga gagaatatga acaaggatga tgaatcatgc gaaaaatgaa aacaatggat
       61 ggcaatacgg cagccgcata tatttcttat gcatttaccg agattgctgc aatatatcca
      121 ataacaccca gttcgaccat ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa
      181 aatgtattcg gtcaaccagt aaaagtagta gaaatgcaat ccgaagcagg agcagctggt
      241 gtggtccatg gatcattgaa aactggtgca ctgacaacta catataccgc ttctcaaggg
      301 ctgcttttga tgataccgaa tatgtacaag attgctgggg aactactgcc aagtgttttc
      361 catgttgcta gtcgggcagt gacgacaaat gcgttgaata ttttcggaga tcacggagac
      421 gtgatggctg caagacaaac aggatttgct atgttagctg aaagcagtgt acaagaagtg
      481 atggatttag cacctgttgc ccatcttgct tctattgaag gagaagtacc ttttttgaat
      541 ttctttgatg gattccgtac gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa
      601 gaattagctc ctctgatcaa tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat
      661 cccaatcatc cgactgttag tggtacgaac cagaatccag acatccattt tcaacaacgg
      721 gaaacgatca acccctatta tgataaatta cctgaaatcg ttcaaaaata tatgaaagag
      781 atcaaccgat tacgaggaac ttcttatgac ttggtgactt attacggagc agacgatgcg
      841 gaagaagtca ttgtttctat gggatcagtt gcacagacga ttgaacaaac gattgattac
      901 ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc atctttatcg tccttttcca
      961 gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa caattgctgt tcttgatcga
     1021 agtaaagaac caggggcagg tggagaacct ctcttattag acgttcaaag tgcgttatat
     1081 gattctgaac ttcgcccggc tgttattggt ggtcgctacg gattggggtc taaagatgtg
     1141 actcccgacc aaatctcagc agtttttgat gaattgaaaa aagacccaag catacgcaaa
     1201 aaacggttta ccatagggat cgtggatgat gtgacttatc aatctttgga accaaaggaa
     1261 agtctggatt tgacagaacc gcaaacattc caggccaagt tttggggatt tggatctgac
     1321 ggaacagttg gagcaaataa atcagctatc aagattattg gagatcacac cgataaatat
     1381 gcgcaaggct atttttatta tgattcgaaa aaatctggcg gtctgactgt ctctcatctt
     1441 cgttttggag atacgccgat acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc
     1501 tgtcacacac cagcttacct gcatagctat gacttggtca aaggattgaa gccaggtggc
     1561 atttttttat tgaatactct ttggagcgac gaacaattag aaacgcatct gcccttaaaa
     1621 ttaaaacgtt acttggctga aaataacatt agattctata cgatcaatgc catgagattg
     1681 gcccaagaag tcggtttggg acgccggatc aatacggcaa tggaaacggc tttttttaaa
     1741 ttagctgaca tcattccttt tgatgaggtg cttccgctgc taaaagaaga agccctgaaa
     1801 agctatggac ataaatcaat ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg
     1861 accgaactgc ttcatcaagt cgcgatccca gaccattgga gaacgttgga gatgcctgaa
     1921 aaaatcaata aaaggaacac tggttatata caagaaatac ttgaaccagt gaatgcacag
     1981 gaaggaaatc aactctctgt tggtacactt gtagcaaatg gaatgacaac tggcgaaatg
     2041 cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt
     2101 agtgatcgct gtacgatgtg taatgaatgt gcctttgttt gtcctcatgc tgccatccgt
     2161 ccatttttag cagatgaaga ggaaatggaa gaagcgccag aaggatttat cgtgagagaa
     2221 atgcgaggcg cagacggcgt aaaataccgt atccaagttt cagtggaaga ctgtacaggt
     2281 tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag ctttagtgat gaagccttat
     2341 gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa tgacattaag acaaaaagca
     2401 aatccagtca aaggaaagaa tactgtcttg tctacacaat tcaatcaacc gcttcttgaa
     2461 ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg taaaattatt gacacaaatg
     2521 ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt cttctatttg gggcggtgct
     2581 tctcctgctt ctccttatac gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct
     2641 ttattggaag ataatgctga atttggttat ggtatgcttc tagcggcaca gactcgtcgc
     2701 gaggctttgg caagttcaat caaagaagta ttgccaaaag tatcagaaga actacgagaa
     2761 ttgatggaag actggttggt acacatgttt gagagcgaag gaacgcagca aagagcagca
     2821 aaactgcaag ctgcgatcga aagcgaacga tcaattgtcc ctgaactgga ggatatatac
     2881 aagagaaagg atctattcgt aaaaaacagc caatggatga ttggtggaga cggatgggct
     2941 tacgacattg gttttggcgg gatcgatcat gtacttgcca gcggagcaga cgtgaatatt
     3001 cttgttttgg ataacgaagt ctattccaat actggcggtc aaacctcaaa agcaacacca
     3061 gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg cttcaaagaa agatctagga
     3121 atgatggcga tgacatatgg aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa
     3181 atgcagacga taaaggcttt tgaagaagca gaaaagtatc ctggaccttc tttaatcatt
     3241 gcctatacgc cttgtatcac acatggtttg cttggtggaa tgaaagaatc gcttaaagaa
     3301 gcaaaagaag ctgtttcttc tggctattgg tcattatatc gatataaccc tgcaatggtc
     3361 gaaaaaaaca agaacccaat gactttggac tacaaaaaac ctgatttctc tgctatgcct
     3421 gatttcatga ggaaacaagt tcgcttttcc tcgctagaga acgcaaatcc aaatcttgca
     3481 aaagctttat ttgaaaagac tatccaagac gctaaaacac gtttttacaa ctatgctgca
     3541 ctgacgggag aattagaaaa aatcaagaaa cgacttgaac cagaggaatc tatcgatgta
     3601 cccgttaaga ctagaaaaga aaaaacgacg gaccttgaag cagaagaaag aagagctgcc
     3661 agagcagaac gacgtaaaaa aatggaacaa gctacaaaag aaaaaaagga aaaataacag
     3721 aaaaaatata atgaataatt ctctgaagtt taaaaatatt tattgcagag aaaaataaaa
     3781 cgcatacttg caattttcct gaaaattatg cgtttttctc aagaaaaata cgagcagatc
     3841 agcctctaaa attaaaacat atgatatact agagaggaaa tctcaaaaga gaagagaggc
     3901 tttttatgtc atttcaaatc ggggaacttt ttaatttgga ctattggcag caaatgtttt
     3961 ccggtgactt gttctctctg aattttttgg tgaacatact tgatattctg gttgtctggt
     4021 atcttgtata caaactgatc caactgctgc gaggcacaaa agcgattcag cttctgaaag
     4081 gtgtagctat ttttattgtt atccgaatcg tagcggaaat cattggtctg cacaccttgt
     4141 cttggctgat gaaccaagtc attacgtacg gggtgatagc tgctatcgtg atttttcagc
     4201 cggaaatccg gagaggactg gaacatcttg gacgcagttc gcttttcaaa caatcaaaaa
     4261 gcgaacagca ggaagatgag aagatgatct tgtcttttga caaagccatc caatatatgt
     4321 ccaagcgaaa aatcggtgcg ctaattacta tcgaacggca tactggatta gatgagtata
     4381 ttgagacagg aattgcatta gatgcagata tcactggaga gctgctgatc aatatcttta
     4441 ttcctaatac accgctgcat gacggtgcag ttattgtaaa agaaggaaag atcgcagtag
     4501 caagcgccta tcttcctcta tcagaaagta tgttgattcc aaaagaattc gggactcgac
     4561 accgggcagc tgtcggtata agcgaagtca gcgacgcgat taccatcgtt gtctctgaag
     4621 aaactgggga tgtcagcatt acattagata atgaactgat ggctggctta tcccagcaag
     4681 aatatttggc aattttgcgt agagaactga ttgctgaaga gaaaacaaaa aacaagaaaa
     4741 acgtgattca atctttccta gacggtatga ctaagggagg ggatcgcaaa tgatctcaaa
     4801 agagaaacgg acaagcattc tttacagttt tattgcattg ctgttcagta ttgcgctgta
     4861 cttcaatgca aatggccagt ccgttccaaa tacgctttct ggaaatgaag cgtataatca
     4921 aacagttact ggggtgccaa tcaagatttc ttatgattca aagcgctact atatccatgg
     4981 atatgaaaat gcagtgactg tcaaactttc cagtgcaaac cgagttcagt tgaatgcaga
     5041 ggcaaacgaa gatacacgga tgttccgcgt cacagcagat atcactaagc tgggcgaagg
     5101 aacacatgag gttccgctaa aaattcaaaa tttgagcagt gctgtcacag caaagatcga
     5161 accatcgact attacagtaa cggttgagaa aaaggtaata aaagatttcg atgtggaaac
     5221 gttgattcct tcaacgtttc caccatctgg atatgaactt gatgatactt ccgttagtcc
     5281 taaaaaagtc tctatcacta caggtgacaa aacattggca gaaatcaaac gagtagtagc
     5341 tgatgtggac gcttcgatgg tcacagatga tgggattaat agtgaggtcc caatacaggc
     5401 gctgaatgca gcaggggaag tattaagtat cgtctctgat ccagtgcaag taacagtgag
     5461 agctgattcg atcaagccaa gcaagtcggt acgactttat gggatacagc aaggaacacc
     5521 agctgccggg gtagaaagct atgatttcag tttttcaaaa ctggaagcag aagtaagcgg
     5581 ctctagcgat ttgcttgcat cgatcggtga cagtattgct gtaccgatca atgtctcagg
     5641 tatcaatcat cgaacaactc gtaaaattga aataccagta gaagaggggt taagtatcaa
     5701 gcccgaatca gttagtgtgg agattacacc cgtgttgcaa acgtcaagca gtagctctgc
     5761 ttcgacgagc agtcggacac aagaaaatac aagtacgagt agaagtattt catctgctga
     5821 ttcaggaacg gaaccatcga caacaaccat ttcttccagt tcggaatctg ggacaagcag
     5881 ctcgaccaca agtacagaag aaacatcaga aacaagcgaa tcaacacaaa tgagcagaac
     5941 aaattcctca gaaaattaag ttaaaggagt aattttacaa ctatgggtaa atattttgga
     6001 acagacggcg tacgcggtga agccaacaag gaacttacgc cagaacttgc atttaagctg
     6061 ggacggtgcg gaggttatgt cctaagccag catgaagaag aaggaaaacg cccacgcgtg
     6121 ttagtagggc gggatacaag aatctcaggt caaatgttag aagaagcgct gatttcagga
     6181 cttttatcgg tgggtatcga agtgttccag ctaggtgtga tctctacacc aggtgttgct
     6241 tatctgacac gtttgcaaaa agcaagcgca ggggtcatga tttctgcttc tcataatcca
     6301 gcacaagata atggaatcaa atttttcggt gcagatggtt ttaaactagt agatgaccaa
     6361 gagctagaaa ttgaagcatt attagatgct gaagtggatg aattgcctcg tccatcagct
     6421 gaaggattgg gaactgtaga agaatttcca gaaggcttat tgaaatattc tcaattcctt
     6481 gtacaaacga tcaacggtga cctttccggt ttgaccattt gtgtcgatgc agcaaacgga
     6541 gcaacttcaa caagtgttaa tcgtttgttt gccgatcttg aaacagattt ttataccatg
     6601 ggcacaaatc cagacgggtt aaatatcaat gatggcgtag gctctaccca tccagaacgt
     6661 ttagctgaga tggtcgttga aaaaggagca gatgctggac tagctttcga tggcgatggc
     6721 gatcgtatta ttgcggtgga tgaacttgga aacattgtcg acggtgataa aatcatgttt
     6781 atctgtgcta aatacttggc agaaaaaaat cgattgaaaa aagatacgat cgttacgact
     6841 gtcatgagta acttaggatt ccacaaagcg gtggaaggaa tcggtttgaa agatgtcgtt
     6901 acacaagttg gtgatcgtta cgttgtagaa gaaatgcgca aaaacgatta taactttggc
     6961 ggcgagcagt ctgggcacat gattttctta gattttaaca caactggaga tgggatgctt
     7021 tcaggaattc agttactgaa tatcatgaaa cagacaggta agaaattatc agagcttgca
     7081 gcagaggtaa caatctatcc gcaaaaatta gtgaatatcc gagtaagtga taaaaacggt
     7141 gcgatggatg ttccggcaat caaaaaagtg attgaagagg cagaagctga aatgggatca
     7201 gaaggaagaa tcctagttcg tccttccggt acagaaccat tacttcgagt gatggcagaa
     7261 gcgcctactg atgagaaagt taattactat gtcgataaaa tcgcggcagt agtcaaagaa
     7321 gaaatcggac tggattaaga gatattttca taaaaaataa aagaacaagg accagtgtca
     7381 gatgtctgac actggtcctt gttcttttat tattctactg tcactgattt cgctaaatta
     7441 cgtggtttgt caacgtcgaa tccccgtaat agcgttgcgt aatatgcaag taattgagtc
     7501 ggtacgactg tggttagagt agttagataa gggtggacag ttggtagcac cagctggtca
     7561 ctttcctttt ctaatccctc tgagacaata actagtgttt tagcgccacg gctttcgact
     7621 tctttcaggt ttcctcgagt atgcgcacca gtcacttctt cactgatgat tccaatcact
     7681 ggtgttcctt cttcgatcaa agcaatcgtt ccatgtttca attcgccagc ggcaaaacct
     7741 tctgcctgga tataactgat ttctttcaat ttcaaagcag cttcaagaga aacgttgtag
     7801 tcagcgccac gtccaatgta aaaggcattt cttgtatcgc tgagatatac ttctgccaac
     7861 tcttctaaat aatctttttg ggcgatgacc gattccatcg cattagcaac taaactcaat
     7921 tcatgagtga tatcgaatac aagagaagtt tcaaattctt ttttatcacc gatagctttg
     7981 gctaataaag ttagtacagc tatttgtgca gtataagctt tcgttgatgc tactgcgatt
     8041 tcgggtccag catgcaatag cagtgtaaag gatgcttccc gcgaaagagt agaaccagca
     8101 acatttgtaa tcgtaagtga agggtagccg agacggttga tttttaccag aacttggcgg
     8161 ctatctgctg tttcgccact ttgactcaag aaaataaaga atggtttttt tgaaagaaga
     8221 ggtgtgttat agccaaattc actggctaaa tgaacctcaa cagggatctg tgtcaatttt
     8281 tccagagtct gtttacctgc taaccccgca tggtaactag ttccgcaagc aacgatatag
     8341 attcgatcgc tatcagacaa tgtatcgacc agcttttgat caagagagac acgattgtct
     8401 tctgttacgt atttttgagc gatacgacgc atgataatag gctgttcgtc gatttctttc
     8461 agcatataaa aaggataggt acctttctcg atatctgaag aatctacttg tgcttcgaat
     8521 gaagaacgtt ctacgatgtc acctgcaaaa ttttgaataa taactttttc tgatgtgacg
     8581 gtaaccatct cttcatcttc aatctcaacg aattccttcg tatgagcaag catagccatt
     8641 gcatcgctgg caatcacatt gaatccatcg ccgagtccga ttaatagtgg acttttattt
     8701 ttagcgacgt aaataacatc aggtgctgtc cgatcaatca aagcgaaagc gtaagaacct
     8761 ttgatgaccc gcaaagcttt taaaaacgcc tcttgcgttg tagtatcctt cgcgaaagtt
     8821 tcaattagat gagcgataat ctcagtatcg gtctcaccaa taaagtggtc atttgctaaa
     8881 taagcaattt taagttcttc aaagttttcg attacgccat tgtgaacgag cacgaagcgt
     8941 ccactttggg aagtatgtgg atgggcgttt tcttcagaag gttgcccgtg agttgcccag
     9001 cgtgtatgac caatacctat atttcctgta gtatcaatat ttattttgtt tttcaaattt
     9061 tgaattctgc cttgtgcttt gaccagataa tcagttgtgc catctgagac aaagatacca
     9121 gctgaatcat aaccacggta ttctaatttt tctagaccgt caatcaatcc tggtgttaca
     9181 ttttttaatc caatcattcc aacaattccg cacataaaaa acatcctact ttctataatg
     9241 aattcgtatg aatgcttcca gctgcaggaa aatccttttg ttttatcttt gcagatattt
     9301 tttgtggatt ttctgtagag tgcagtccct agggccatcc gccgaatctt tcgataaacc
     9361 ctctcctcgt catctgttta cacagacctg gcgctatcct aaaaggtata actatcctcc
     9421 tcttttgttt tttatctgga aacatttaaa atcataacct tttgcgaatg ttcttgtcaa
     9481 caaaaaaaga ataaaaaata aattggtata tatcaaatgc tttgtcaaat gctcttttta
     9541 tgtcaaaaaa taatgggttt ttatctatca tctttttcag gagttcggta caatagaaag
     9601 taatcggata gaggaggagt aaaaatggat tcaaaaattg agatcatgac actgggaatg
     9661 ttaaaaaaac aattgtccaa attcgaagca tcagctggtg tatcagatga tacgaaaata
     9721 tttttagata ctggctggga tagcatccaa gaaatcgcac ctgatgcctt ggaagttgct
     9781 gaagctcgtg agtttacggt agaagatgaa ttgacaaaag agtcgttttc cgggtatgcg
     9841 cgggaagaaa aagcagagcg gttcgacact tctgagcagt cagaaactgt gatcgtcata
     9901 aaaaacttgt attagaaagg agaaagaatg aataagagac gttgggcagc agttctgatt
     9961 gctgcaggat tatttgtggt ttcgttagtt tcagctagtt tgactaagcc tcaggaaaac
    10021 gatcaaattg agggattgaa cagttggtta tatggggatg aagaactagc ttcaaatgtg
    10081 ttggaagagg gagacagtac gcaaaaaatc gtaaaattat cagtagatgg aacgattgct
    10141 gcgggagggg cttcaggttt atttgcttct gaaggttaca atcataaaaa ttttatggaa
    10201 caactgaagg cgattgaaga agatccaaca gtcaaaggta tctttttaga agtaaattct
    10261 cctggtggcg gtgtctacga aagtgcggaa atcgctaaga aattggatac gattcgtaaa
    10321 gaacatgata tcccaatgta tgtcagcatg aaaaacatgg cagctagcgg tggctactat
    10381 atttctgctc aagcagataa aatctttgca acagaagaaa cagtgaccgg ctcaattggt
    10441 gtgatcatgt caggattgaa ttattcagga cttctagaaa aactgggtgt ggaagataca
    10501 acagttaaaa gcggagcgtt gaaagatatg ggttcaggaa cgcgaccaga gacaaaagaa
    10561 gaagaagcgg tgcttcaagc atatatcgac agcgcatata gtcgttttgt agcaatcgtc
    10621 agcgagggac ggaataaatc tgaagaagaa gtcaaaaaaa ttgctgatgg aaggatttat
    10681 gatggtgtcc aagcaaaaga agtaggttta gtcgatgaat tgggatttcc tgaagatgct
    10741 ttaaaagcta tgcgaaagga acaacagctt gaagatgcgg aactagtaga atattcttcc
    10801 acgccatctg ggtttgctag tacatggtta ggaagtaaac tagcagaatt ccagggattg
    10861 aagccttctg agactagtca gatcttatcc gttcttgaga atctagggac agcggaatca
    10921 ccaaaagcaa tgtattatta cggaggtgag taagcatgtc agagctttca aatgaaaata
    10981 aggaacaaga aaaagataag caacagcagc ctgctgtagg acctgactcc aacttttccc
    11041 aaaaggtact gagctctttt gaaagagagc ctttgacgcc cgaagaaatc aaaaagaaac
    11101 aacgtaaatg gaaaaagtat acggacacaa gaaatgaacc gccaaaagca aatgattttc
    11161 ctaagtattt ctatgcaggt ttttttatcc gaatgtttgc tttcttggtc gatcttttgt
    11221 gtatcggagc tattacgcgt ttaacacttg gtattgccaa aaatctaggt tggcttaatt
    11281 tttcagattc ttatctgagt atctacggat tcacagcctt acttatttat ctagcgtatt
    11341 ttattttatt gacgaaattc aatcatggtc aaacgattgg gaaaatgatc tttggtattc
    11401 gagtcatcag ctttgatgaa gaagaactta gctggtcaac cgttttagtt agagaaggtg
    11461 cttgccggtt cattctaaaa tttcctttgc tgatgttagg gtatcttcca gcaatcttta
    11521 atcagaaaaa gcaacatcta ggcgattttt tcactaatac aagtgttgtt acgatcaatc
    11581 tgatcaaagc attcaaccag gaagtcaaca gctgatgagc aataaaaagc agtaacagtt
    11641 gaaagcacct tctaaaagtc agatctaaaa tctagctttt agagggtgct tcagttattt
    11701 ttaattgtca tgcttttttt gttttgctac tattgaacat gagctgaaca tgagcaagag
    11761 gaggacactg cgtgaagccg attgattttc cagaaaaata tgagaattta atgcgagtag
    11821 cacaacaagc actagcaaac cagcaatatc agcaggcaaa agaattattt caacgagctt
    11881 acgagttgaa agaaagcttt gaggctaata gcctgcttgt tttttgtctt tacgagttgg
    11941 acgagaaaaa agaagcattg aagcaagcac tcttgcatga aaaacagtat cttcaaaatg
    12001 aggagtttgc tgaattttat tttgacttgt taatcagtac acaagatttt ttatatgcta
    12061 gaaaattgat agcaagtaca gatttttatg aagctttcga gcagcgggtc attgaaaaaa
    12121 tccaatttgc agaagaatta tccggacaaa tggagaggca gaagatcaag gcactccaca
    12181 ataaatcgga ggaacttcct tcgttggaac ctgcacgtca gcttgcgtct attgatgaga
    12241 tcgaacagct tccttatcat gagtttatcc aaacagcgag taaacttatt gtatcgccag
    12301 aggtccatat tttagcaagg gcaaaattac tggagacact taggcaattg aacgaatgcc
    12361 atcctgtttt ctatttgacg attgaagaaa agttagtcaa agtcatacca aaagatctgc
    12421 caaagccgca gcagcaggca agttaccagc aattatgtgt attttcagat cgttacggta
    12481 atgaagatgc gctgcttagt tccgtgttaa atgaagaatt cacgttgcaa tctgcaattg
    12541 tttatcctgt ctatgataca tacatcgaag acccgaagcg ctggtttcaa ctgactgtag
    12601 aggcgtgtac aggaaaaacg atgtatgaag gtacagaaga tgaaaaacaa gattttttaa
    12661 aaaaaagaga aaaaattttg cagcaaatga tcttcttcca ttagatttta tcagtgaaaa
    12721 agataaaaat gttatgaaaa acaaggattc tatccattca atatgtttac aatttgaaag
    12781 ggttagtgta ctatttaaag gtatgcatga aacgtttttg atgtataaaa tgaatttcta
    12841 aactcggagg gaattaaatg actgcaacat ttgaaaaaaa aggcacgaat gatggtgttt
    12901 taaaatttac gattgcgcaa gacgaaatcc aaaaaggatt aacaactgcg tttaataaag
    12961 tgaaaaaaag cttgaacgta cctggattcc gtaaagggaa agtttctcgt caagtattca
    13021 accgtatgta cggtgaagaa gctttatatg aagatgcatt gaatgctgta ttaccagaag
    13081 cttatgaagc tgctgtcaaa gaagctggca tcgatcctgt ttctcaacca aaaatcgatg
    13141 ttgaaagcat ggaaaaaggt caagactggg taatcactgc tgaagtaaca gtgaaaccag
    13201 aagtaaaatt aggacaatat aaagatctag ttgttgaaaa acaagaccgt gaagtaacag
    13261 atgctgacgt ggatgcacgt atcaaacgtg aacaagaagc acaagctgaa ttagttatca
    13321 aagaagatgc tgcagctgaa aatggcgata cagtagtaat cgacttcgaa ggctttgtag
    13381 atggcgaagc gtttgatggc ggaaaaggcg aaaattactc acttgaatta ggttcaggct
    13441 cattcattcc aggttttgaa gatcaattag ttggtcataa atcaggtgaa agtctagatg
    13501 taaatgtaac tttcccagaa gattatcaag cagaagatct tgctggaaaa gaagcagtct
    13561 tcaaagtgac aatccatgaa gtaaaagcaa aagaagtgcc tgaattagat gacgaattcg
    13621 caaaagatgt tgatgacgaa gtatcaacat tagacgaatt gaaagaaaaa tatcgcaaag
    13681 aattagtaga aacaaaagaa aaagcagcgg acgatgcaaa agatgaagca gcgatccgta
    13741 tggctgttga aaacgcagaa atcgttgaat tgccacacgt gatggttcat gatgaagtac
    13801 atcgttcaat ggacgaattc ttgaacaaca tgcaacgtca aggtatctct cctgaaatgt
    13861 actatcaatt aactggttct acagaagaag acttgcataa acaattcgaa ggtgaagcag
    13921 aaatgcgcac aagaacaaat cttgtgatcg aagcaattgc agctgctgaa aatcttcaag
    13981 caacagaaga agaaatcaat gcagaaatcc aagaattatc agaaacatac aacatgccaa
    14041 tcgaacaaat caaacgcgtg ttgacagaag atatgctgga acatgatgtg acaatgaaaa
    14101 aagcaatcga attgatcaca ggtacagcga cagaagcgaa agaatagaaa tcaagtaata
    14161 aatcatttct gaagaagcta cttctagaaa attgtttatt cgaaataagg gactgtgaca
    14221 cgattttgtc acagttcctt attttttgtt aagagtaaaa aatgcaaagg tataggtcag
    14281 ttgatccttt gttaaacact ttttcttttt actaaactat gttatgatag taaaagactg
    14341 taaagtcttt gatagtttct tgcatacttt ttgcaagtta aggggtgaag accatgtatg
    14401 acaatccaag tagcaatgag accgtacgct gttctttttg cgggaaaacc caagaagaag
    14461 tgaaaaagat cgtagctggt ccaggggtct acatctgtaa tgaatgtatc gacctatgta
    14521 aagaaatcat tgatgaagaa ttttatgaag aagctgtacg cgaattcact gaagtcccta
    14581 aaccactaga aattcttgaa gtattgaata actacgtgat tggacaagat cgcgcaaaac
    14641 gctcgttagc tgttgccgta tacaatcact ataaacgtgt caatcagcaa gcacaagaaa
    14701 acacagatga tgttgaactg caaaaaagca atatctgctt gatcggacct acagggtctg
    14761 gtaaaacatt tttagcacaa acattagcaa aaacattgaa tgtacctttt gcaatcgcag
    14821 atgcgacaag tttgacagaa gctggttatg tgggggaaga tgtagaaaac attttgctta
    14881 aattgcttca atctgctgat tacaatgtgg aacgtgcaga aaaagggatc atctatattg
    14941 atgagatcga taaaattgcg cgtaaaagcg aaaatgtatc tattactcgt gacgtttccg
    15001 gcgaaggtgt ccaacaagcc ttgctgaaaa ttctagaagg aactgttgca agcgtgccac
    15061 ctcagggagg acgtaaacat ccacatcaag aatttattca gattgatacg acgaacatat
    15121 tgttcatcgt aggtggtgca tttgatggca tcgaaacgat tgtgaagaat cgtatgggcg
    15181 aaaagacgat tggatttggt acacaaaacc aaaaactttc tgaagacgaa agtgtcatgc
    15241 agcatatcat tcctgaagat ttactaaaat tcggattgat ccctgagttt atcggacgat
    15301 tacctgttat ggcagcttta gagaaattga caacagatga tctggtacgg atcttgactg
    15361 agccgaaaaa tgcgctagtc aaacaatatc aaaaacttct ttctctagat aataccgaac
    15421 ttgaattcga acctgaagcg ttgagagcca ttgcgaaaaa agcaatcgaa cgcaataccg
    15481 gtgctcgagg acttcgttcc atcattgaag aaatcatgat ggatgtgatg tttgatatcc
    15541 cttcaaatga aaacatcgaa aaagtgatta tcacaaaaga agcagctgaa ttgtctggtg
    15601 agccaacagt aatctataaa gattcagaga aaaaagcggg ttgatgaaaa gaggctgccg
    15661 taaaagtcat tttggctttt gcgacagccg cttgctttat tcatcacctt gttaaggaga
    15721 gagaccatga aagtccatca agctgaaatt atcataagtg cagtggcacc aaaacaatat
    15781 ccagatacac aattgccaga aatagctctg gcaggacgat caaatgtagg gaaatcttct
    15841 tttatcaaca cattgattga tcgaaagaat ttagcccgta cttccggtaa gccaggaaag
    15901 acccagacat tgaattttta tttgatcgag aacgcattac attttgtcga tgttccggga
    15961 tatgggtatg caaaagtatc caaaactgag cgagcaaaat ggggaaagat gatcgagaca
    16021 tatttaacga ctagagacca gctacgtgcg gtcgtttctt tagtagattt gcgtcataag
    16081 ccttctcaag aagatatcca gatgtatcaa tttttgaagt attatgatat ccctgtaatt
    16141 gttgttgcca ctaaggcaga taagatccct cgtggtaaat ggaacaagca tgagagcgaa
    16201 atcaaaaaag cgttggattt tgatcgcaat gacacgttta ttctcttttc ttccgttaca
    16261 aaacagggaa aagaagaagc gtgggcagca atcgaacaag caattgaatc ataaaaagaa
    16321 taaaaaagac ttaccagtca aactctttca taaaagtttg gctggtaagt ctttttttga
    16381 gcttaactta cttttgtttc ttgtcttttt tcttttcatt ttgtggctgt ggttttagaa
    16441 atcgatcgat ctcttctttt ggtgcaactg ttttttccgg atcgacagca aatgattcga
    16501 aaagtttatc taaattatct gtacgttcaa atttcaaccc cattgtaaac acctctttgt
    16561 ttctttttac ttagtctatc atgtaatcgt ccaaattggt ttctaagaca taaagtttta
    16621 gtttttctt
//
LOCUS       DACCWV010000058        16346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCWV010000058 DACCWV010000000
VERSION     DACCWV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.058"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(131..787)
                     /locus_tag="IVW84_001581"
     CDS             complement(131..787)
                     /locus_tag="IVW84_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7917777.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            980..1258
                     /locus_tag="IVW84_001582"
     CDS             980..1258
                     /locus_tag="IVW84_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917778.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1277..2485
                     /locus_tag="IVW84_001583"
     CDS             1277..2485
                     /locus_tag="IVW84_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7917779.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2633..2998)
                     /locus_tag="IVW84_001584"
     CDS             complement(2633..2998)
                     /locus_tag="IVW84_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7917780.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3145..3918)
                     /locus_tag="IVW84_001585"
     CDS             complement(3145..3918)
                     /locus_tag="IVW84_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7917781.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4092..4952)
                     /locus_tag="IVW84_001586"
     CDS             complement(4092..4952)
                     /locus_tag="IVW84_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7917782.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4952..6370)
                     /locus_tag="IVW84_001587"
     CDS             complement(4952..6370)
                     /locus_tag="IVW84_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7917783.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6427..6876)
                     /locus_tag="IVW84_001588"
     CDS             complement(6427..6876)
                     /locus_tag="IVW84_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7917784.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7321..7584)
                     /locus_tag="IVW84_001589"
     CDS             complement(7321..7584)
                     /locus_tag="IVW84_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7917785.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7683..8978)
                     /locus_tag="IVW84_001590"
     CDS             complement(7683..8978)
                     /locus_tag="IVW84_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7917786.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8975..10246)
                     /locus_tag="IVW84_001591"
     CDS             complement(8975..10246)
                     /locus_tag="IVW84_001591"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7917787.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10411..11280)
                     /locus_tag="IVW84_001592"
     CDS             complement(10411..11280)
                     /locus_tag="IVW84_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7917788.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11572..13182)
                     /locus_tag="IVW84_001593"
     CDS             complement(11572..13182)
                     /locus_tag="IVW84_001593"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7917789.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGAVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14375..15715)
                     /gene="glnA"
                     /locus_tag="IVW84_001594"
     CDS             complement(14375..15715)
                     /gene="glnA"
                     /locus_tag="IVW84_001594"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7917790.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15743..16135)
                     /locus_tag="IVW84_001595"
     CDS             complement(15743..16135)
                     /locus_tag="IVW84_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7917791.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4663 a         3465 c         2781 g         5437 t
ORIGIN      
        1 cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
       61 gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
      121 tctttgttca tcaaaagata atattgtctt ctaacaagtc catattcaaa ataacgatat
      181 gatggtcttt aacggtgata ataccatctt ttttcagttg ttttaaaatt ctgtttacac
      241 tgctagcagt tgagatgcca caaaatttgg cgatttcttc attcgtgatc gaaaaatcga
      301 tcagcatccc ttcttctgtc cgcacaccga ataaatcata taattcgtaa agttgcgtac
      361 aaacagctcc cagctttccg tttaatagca tttgctgcat ttttttcatc gaataaagca
      421 gacgatagcg gtaatactct tttacatagt gttgtagctt tgggtcttgg ttgatatttt
      481 gccaaaagcg cacacgattg attcgataaa actctgcata ttctgattca actcgaatat
      541 tatacggcga atcgatgaat tgtgaatatt cgtcttttaa aagagagacg atttcaagat
      601 ctttgatata gcgaaggtta aattctctcc catcatttga aataacactt gcttttaccg
      661 ttccctcttt caagatatac gtatgggaat cttccatccc ttcgaatgtc aaatatttat
      721 gatatccttt gcgtataatc ggaaaatcat ttttatccaa atattccttt aagactctac
      781 tatccaatga tgctccccca tccaaacttt catctaaaaa gatcatacag tactttaaaa
      841 ctttttaaag tccaaaggta ttttactaat gatattaaca tttgttaagt acctttttcc
      901 atcacttatg cgattttttt cctttttttt attcgttata ctgcagaagt aaaatttttt
      961 aagaatagag ggaaaaaaca tgaattactg gatgaacaca atcattaatc gattagaaac
     1021 agcatatcaa acaagatttg atatgaaagc ttctttagtc tttttaaatg atgcgtatca
     1081 aaactcgatc gaattgatca aagcagtgga tgagaatcca acaaacgaat gcgaagaatt
     1141 tttaaatctg tttatgtcta caagagattt atttattcgc caacttgttg atcgctatcc
     1201 atccaattat cacgatgtag aagttcaaat acaaaaatta aaagcatatt cagcttaagg
     1261 ggaatgtata acatacatgg aaaagaaatt atttaataaa tattttattg gaatcacatt
     1321 gatcaatttt attgtctacc tgatttatta tctgttgatg gtcatcatgg cagtcattgc
     1381 gcaagacacc ttacacgcca gcttgagcca agcaggtttt gcttctggga tctatatcat
     1441 cggaacattg ctggcacgct tattcatggg gaaacagtta gaactatttg gtcggaagtt
     1501 taccttaaga ggcggcgcga tcttctatct cctttcaaca ttagcatatc tttatacgcc
     1561 aactatcggg gcattgtatt tagttcgatt cttgaatgga ttcggctacg gtacagtttc
     1621 tacagctaca aatgctatcg ttactgcata tatccctgca tctaaaaaag gggaaggcat
     1681 caattattat ggattaagta ctagtttagc tgccgctgtt gggccatttt taggaatgat
     1741 tttgctgaat atgactgatt ttcgtttcat tatctggttt tcaattgtac tggtcttctt
     1801 tgttacgatt gcctgccttg tctttcctgt aaaaaatatt ccattatctg aagaacaaaa
     1861 aatatctctt cgctcatgga atatcaatag ttttattgaa aagaaagcac ttttcattac
     1921 aggaattgcc tttttgatgg gtctttctta ttcaagtgtt ttatcatttt tatcttctta
     1981 tacaaaagta atccacttag ttgctgctgg gtcatttttc ttcgtcgtat atgctttggt
     2041 catcacattt acacgtccat tgactggaag gattttcgat gtcaaaggag aacaatacgt
     2101 gatgtatcct agctatttat tcttaacagc aggattattt ttactgagtg taacgacgaa
     2161 tagtgtgaca ttgcttatct ctggagcatt agtcggccta ggttatggta cgtttatgtc
     2221 aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta ttgctttatc
     2281 tacctacttt attggtttag acctaggctt aggcgttggt ccttatttga tggggatgct
     2341 aaaatctctt acttcattta gaggattgta tgtgattgcc ggagtcattc cattgatatg
     2401 tacagcactc tatcttgtta atggccgaaa acatagtcat gaagcagagg gcgaattgat
     2461 tgaatcattg aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc gttcagatct
     2521 gaaaataatc tgagcggttt tttgagtaaa aatagtttat gattattctt aattaaacat
     2581 ctatttattt ttaaagcttt catttatcga taagaatagt ttaactgttg atttattcca
     2641 ttttccgttc atctggtacg atgccttgac ttagcaacca cttgtggaag aaccattctg
     2701 ccacaccgat caatagagca ggaatcaata ctttcatcca aatatcgact gtatcattca
     2761 taaaatatag atagagccac aagataactg cagctgaacc agcatctgcg atcgtcgctg
     2821 cgatgttccc tactttacgt aagatcaaca gatcccctag cacatagatc atgactacaa
     2881 taattacccc aacaatcgtt gaatccataa agctaacacc gtataaacct gtcagcacga
     2941 cccacagaat cgcccagatc atgattgcct tcactaccaa cgccttcaca tgtttcattt
     3001 tctgcactcc tttctcctgg ataaaaaagt aagattatct aacaagataa ccttttcatt
     3061 attataccat cggctgagag aagcgagcaa agataaggtc ctatctaaaa atgaggaagc
     3121 cacttagaac attcacgtaa taatttatcc gtacagacat tcgatatggt gttcctttac
     3181 tttcgcatac tgttcttttg tcagttcttt acttgtcacc agataatcga tttcatgaaa
     3241 agcaatggct tgatgcatga atttatgtgc aattttttgc tgtgtaacta aaacggcaag
     3301 ccggtctgat tgttttctaa aagctttttt gaccattgcc tctccttcat gagtttctgt
     3361 tactccttgg ctgaaatcta gcccgcgaca actcattatc gctaaatccg cataatatga
     3421 caatgcatcg ttataggctt ttgaaccgtt gattgttgct cgtttcggaa caattgcccc
     3481 tcccaataca gatacttttg ccgttgtata ttcactcaac atagcagctg tgacgatgcc
     3541 attcgtaatg atctgcaaac catcaaatct tgctaggttt ctgactaagt gtaaagaggt
     3601 tgtactggaa tccaagaata agaccatatc atctcgaatc aaagaagaag ctgtatctgc
     3661 aatcttacgt ttttctgttt cttcttctaa tttggtgatc ttcatcaggt aaggcacgtc
     3721 aatttttcga ttgtcaataa gcatggcgcc accgcgaact ctacggatca atccactttt
     3781 ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta taatttacta actcgatcaa
     3841 atcggtgact cttgcaaatt ggttgttttt tagataattc acaatctcct gctgtctatt
     3901 ctccacttca ttcaacataa aatcacccca tttactttat atagtaacag gaaaaaagag
     3961 gttgtgacag aagtgattgg cttcgagaaa taagaagaaa tttttcgaaa aaactacttc
     4021 tgaacatcgt gtgtggttaa gagggtatga caagactatt gtcataccct cttgaccaca
     4081 catttttatt tttagtataa actagccttg cctactgtgt tcaaaatttc cattttatgt
     4141 ttgaccactt cttgtacctt tttattagct gctggataaa ttgcattcgg ctcgtacatc
     4201 gatggatttt cactcaatat ttctctcaaa gcattgaaaa agacgctttt aagatctgag
     4261 ctcaaattca ctttggcaac tccgtatttt actgcttcgc tgacttctgc gtccggatta
     4321 cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt ggttcaattc tttcagtaag
     4381 tcgatattta attttggagt tctgtttttc ggatacaagc catgcgctgt tccaattgct
     4441 acagcaagtg tatcgattcc tgtttctttc acaaaaatct tcgcttgatc aggatctgta
     4501 taaatgattg tttcagcacc tccttcagca gaaccattat tcccaatcgt tccaagctca
     4561 gcctccactg agacatttac cgcatgagct aacgcaacca cttttttcgt aatctgtaca
     4621 ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg aagaatagcc gtttcgaatc
     4681 gcccgtaaga catcttctat cgtaccgcca tgatccaagt ggataacgat cggcagttta
     4741 cttgtataag cataggctct tactgccgca acaaactcat cacttaaata ggcgatttca
     4801 tcaggatgaa tttctaagat tactggtgct tctaatctct ctgcttcttc gatgattgct
     4861 ttcatcatat catagctgca aatattgaag gctggtactg caaattgatt ctctttagcc
     4921 actttcaata actctttcat tgtatataac attacgcttc cacctcattt gttagatttt
     4981 catttttcgt ccacttgtct tgttttttct ctttcactgt ttttttcaat agtccaagca
     5041 taaaggctgt agtgactgta cctgatgcaa ttgccaagac atacataggt aaattgttca
     5101 taacatttgg aattagcatc acccaaatcc ctccatgagg tgccattgaa gtaactccta
     5161 ctgccatgga aatccctgca gcgactgctg aacctgtcat caaactaggt atcacgcgga
     5221 tcgggtcaga taccgcaaac ggtatagcac cttctgtaat atacgaagcg cctaaaatcc
     5281 agcatgattg acctgaagtc cgttcttctt tggtaaattt atgtttgaat aataaagtgg
     5341 ctaaagcaat acctagggga ggtgtcattc ctgcagccat acaagcagca atcggtgcat
     5401 ttactccagc tgacatcaaa ccgatcgaga aagtcgaagt cgatttattg atcggcccac
     5461 ccatatcagc tgccatcatg atcccgataa gtaatccgaa caatagcccg gatgtatttc
     5521 ccaagctatt cagccaattt gtcaaagcat ccaacaccca tgtgatcggt tgttcaatca
     5581 caaataccat caaaagccca ataattaaga tcgataaaaa ggggacgaca atcagctgat
     5641 aaatagcaga aaattctttt tttacgctaa ttttatttgc cagaaaatca cagatatacc
     5701 ctgctagcac gccaccaatc attccgccaa gaaaacctga accacctgta gaggccagta
     5761 atccggcaac aattcctggg gcaaaacctg cttttccagc aatcgatgta gcaatccccg
     5821 cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc tccgatcctt gaaaatgcag
     5881 ctgctaacac attatagtct gctgagttgg gatcagccgc tgtaatccca aaggcaaaac
     5941 tcagtgcgat gatgattccg cctgcgatca caaatggaag catgtaattc acaccattca
     6001 tgaaatgatt atagattgtc ggctttttct catttgcagc tggttctttt tcattagaca
     6061 caacgaatac gccaatatct ctcaatgctt cgtctattac tacttgcggt tctttgattg
     6121 ctcggcttgt agagacacgc tttacttttt tcccggcaaa tcggtccaat tcgatttctt
     6181 tatctactgc aagaatgatt gcatctgcct ctttgatttc ttgttcagat aactgatttt
     6241 ctgtttttac accttgtgtt tcgaattttg cttgatggtt tagatttctt gctgttactt
     6301 ctaatttttc tgccgacata taagtatgag caattccagt cgggcaagca gttacaccaa
     6361 cgattttcat gattattttc ctcctttttc ttcactatcg atactcggac aaagactcta
     6421 ctattttcat tcttgactaa tctgtctgat gatatctgct ggcgtagtgg cttcttttat
     6481 tccctttcta aaatctgcat tcatcaatcg tctggagagt gtgctgagaa tctctagatg
     6541 catatctgct gatttttcag gaactacgat ggcaaaaata tattcaattg gcatttcatc
     6601 tatcccgcta tagtccgtta atttggctgt atgaaaaaaa cacagtgctg gttttgtcac
     6661 atatttgctt tttgcatggg gaatcccaaa tccatcttct attcctgtgg agatctcctc
     6721 ctctcgtttg aatagtgcct taacgacttt ttgaacattt cttaccttat ttgttttttt
     6781 caacacatcc cctattcgct caaataatga tccttttgaa tctatcgatg gatcgaaaac
     6841 aatcagttct tctgcgatca attgatcaaa cctcataaaa aacctctccc ccacaaaaaa
     6901 tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc tagctgtacc gttcaatatt
     6961 atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat aagtcattaa aatgaacgat
     7021 ctagctcaca aacaatcgca agtctataac aaaacaaaaa gccttccttg aaaggaaagc
     7081 ttagcttcta attttaggta aatggttttt tacttcgaaa taacccctct tcatttcgtt
     7141 aatataaggt gtttttatca agtggataat taaaactata tactagtttt tcattgtttt
     7201 gtgcattatg atcctacgac tttttcagca tcaaacgaag ttttaaattg gcaaaaaaca
     7261 taaaaaaaca gactatccct atcagatagt ctgctaaacg ttgagttatc agcgttttta
     7321 ttattccgca gcgtttgcgt ctttgagacc gtattttttg ttgaaacggt ccacacgtcc
     7381 gtctgcttgt gtgaattttt gacgtccagt gtagaatgga tgtgagtcag aagttacttc
     7441 gacacggatg actgggtatg tattaccgtc ttcccattca actgtttctt gtgaagtttt
     7501 tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc atgaatacta ctgggtggta
     7561 atctggatgg atgttttctt tcatgatttt ttcgctcctt tgccctgctc catttgctga
     7621 accagagttg atttgtcgat ttacaacatt aacatcttaa catgtttcag tcaaggttgc
     7681 aattatcttt tcagatttct ggtcggattt tttttaccaa aggagacatc ttgaaattct
     7741 ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc ggatcagttg ttccgtatat
     7801 tctaatgaat ctccagtcat attttttcga agtttccaaa tttcttctaa ttcttcactt
     7861 ggcatcaata attcttcttt tcgggtacct gattttttga tatcgatggc tgggaagatc
     7921 cgacgttcag ataattcacg tgaaagttgt aattctagat ttccagttcc tttgaattct
     7981 tcatagatca cgtcatccat acggcttcct gtatcgacta aagcggtcgc taaaatcgtc
     8041 aaactgccac cttcttcgat gtttcttgct gccccgaaaa atttcttagg tttatacaat
     8101 gcggctgggt caatcccgcc actcaatgta cgtccgcttg gtggaacgac taagttatag
     8161 gcccgagcaa gtctagtaat actatccatc aagataacga catctcgttt atcttcgact
     8221 aaacgcattg cgcgttccaa tactaattca gaaactcgag tatggttttg cggctgcaga
     8281 tcaaatgtag aagataccac gtcgcctttc acgcttcgtt ctagatccgt tacttcttcc
     8341 ggtcgttcat caatcagtag aacgattaat tctacttccg gatagttttc ggtgatacca
     8401 tttgcgattt cttttaatac agaagttttc cctgcttttg gcggtgcgac gatcaatcca
     8461 cgctgaccaa aaccgattgg agaaaagata tcgatcattc gggtagatag tctttgcggt
     8521 gttgtttcca gttgaagctg tctttgcgga taaagaggtg ttaaggctgg aaaatgcgga
     8581 cgttcttttg cttcttccgg atcttttcca ttaacgcttt ccacgtgcat caaaccataa
     8641 taacgttccg attctttagg tggacgggct tttcctgcca ctttgtcccc atttctcaaa
     8701 ctgaatcgtc tgatttgtga ggcagaaata tagatatctt ctgcacttgg tccataattg
     8761 attggccgta aaaaaccgta accatcttga ccaacgatat ccaatattcc ttccatataa
     8821 taaaatcctt gtttctctgc ttgtgcacga atcacagcta gagataattc ctttttattc
     8881 atcttactgt aataaggaat tttaaattct ttggcataac tataaacttc ttttaacgtt
     8941 ttgttctcta gttctgccat tgtcaaataa tcgctcattc agctcccact tcctctatca
     9001 tttcaattga tgcccctaag gctgttagtt tctcgataat atgatcataa ccgcgtaaga
     9061 tataatctac attgtaaatc gtcgttgtcc cttctgccaa tagtccagca atcactaaac
     9121 aagcacctgc ccgaagatca gaagccacga cttctgtgcc gtgaagttta tttggaccat
     9181 tcacgatgat catatttcct tccacggtaa tatcagcgcc cattcgtgct agttccggca
     9241 catggttcac acgtttcgca tagatcgtat cgatgatttc tgaagtgcct gtagtcatca
     9301 atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa tcctggataa ggataggtca
     9361 tgatgtttgc gggttttaat tctttcgagg ggtaaacttc gatttcatct tcgctgattt
     9421 tcatatggac accaatttcc tgcaattttg ctataaagct ttccaaatgt tcaaagatca
     9481 catttttcac tttgatacca tttcctactg cagctgctaa tgctagatag gttcctgctt
     9541 cgatgcggtc aggaatcatg aaatgccgac agccatgtaa tgtttcgact ccttcgatac
     9601 ggatcacatc tgtacccgct cctcgaactt ttgcccccat attattcagt aaagttgcca
     9661 catctatgat ttccggttct cgtgcagcgt tttcaattac tgtctgtcct tttgctttta
     9721 cagcagctaa catcacattg atcgttgctc cgactgaaac cacgtccatg aaaatccgat
     9781 tgccacgcaa tcctttattt tctgtccgta aatacatggc accatgttca tttgtcactt
     9841 cagcacctag agcttcaaat cctttgacgt gtaggtcaat cggtctaggg cctaaataac
     9901 agccacctgg caggcctact accgcttcgc caaatttacc aagcagtgtt cccataaaat
     9961 aataagaagc acgcaaacta ttgatttttc cgtgcggcat cggaatcgat acgacttctc
    10021 gaggatcgat ttctagtatg ttgttggaaa aatggacagt tgcgcccatg atctctaaaa
    10081 tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc ttccaagatc actggggaat
    10141 cagctaagat tgcagctgga atcaatgcga ctgcactatt ttttgcaccg ctgatcgtta
    10201 cttcaccagc taaaggtcgg tttcctttga ttacgatttt tttcatctgt ttccaccttg
    10261 tttattaatc ttttttcatg gatatgcttg tattttatca tagataaaaa aaagaaacga
    10321 gcatggtctg ccgttttaag taaaaaagaa tggagagcga cttcaaaagt cgttctccat
    10381 tcctatatat cgtatctgtt atgtggatgc ttacgctttg ttagctgaac cgaaccagtc
    10441 gatatgagat ttagcatctt caacgattgc tgctttacct ggagctaata atttacgagg
    10501 gtcgaatcct ttaccttctt tatctttacc agcttcgata tatttacgag tagctgcagc
    10561 aaatgataat tggaattcag tgttaacatt tacttttgag atacccattg agatagcttt
    10621 ttcgatttga tcttgaggaa tacctgatcc gccatgtaat actaaaggca tgtctggacc
    10681 tacagcatca gcaattgctt gtaagtggtc aaaagctaat cctttccagt tttctggata
    10741 ttgaccatgg atgttaccga taccgcaagc taagtagtcg atacctgtag caaccatttg
    10801 tttgcattct tcaatatcag ctaattcgcc gttaccgatg attccgtctt cttctccgcc
    10861 gattgaacca acttcacatt caactgagat accttttgca tgtgcttttt caacaacgtc
    10921 tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt
    10981 atagcctaat tcgatacatt ccaaagcagc ttcgtaatca ccatggtcta agtggatagc
    11041 aactggaaca gtgatgttca ttgcttcaac taaatcagtg attaaatctt tacagatttt
    11101 gtaaccaccc atgtatttag ctgcacccat tgatgtttgg ataagtactg gtgcttgttt
    11161 tgcttcagcc gcttcaagga ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc
    11221 gacagcgtaa ccgcctttac gagcagcttt taaaaattca gctcctgata ctactggcat
    11281 aataaaattc ctcctaagtt ttgtaaaact aaatttgtta agacaattct taaccaacga
    11341 ccctatttta ccaaacattt cggtagtttt ctactaaaat caatgaaaat gaaaattttc
    11401 aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt taatcgaaaa aagtcttttg
    11461 ctttctatct tttttcctac aaataataac aaatcagaaa aagagaaaag tgacaagaaa
    11521 attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa attattttgt
    11581 ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg
    11641 acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct ctggcagctc
    11701 tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc cttgttcttc
    11761 aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt cttgcacaac
    11821 atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac atgggtataa
    11881 tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca ttaaatcaat
    11941 aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta atccaacgac
    12001 gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta aaaacggaac
    12061 gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc gatcaccaaa
    12121 tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat tttctctcgt
    12181 taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga aaccagaatg
    12241 tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt taccgacaag
    12301 cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt tccattctgt
    12361 catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat ccattccttg
    12421 tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact cgataactgc
    12481 ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag aaacaggttg
    12541 ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct cttttacact
    12601 gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa tcaatgttgt
    12661 atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa gtgcttcaag
    12721 gaatggcaaa gattctatat cccctactgc tccccctact tctgtaatga tcacatctga
    12781 atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa tatgaggaat
    12841 aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa cttcagaata
    12901 gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa aacgttcgta
    12961 gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt ctccgtgctg
    13021 atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt ggatagttac
    13081 ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc cttttccgat
    13141 tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa aaactccttt
    13201 ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc ttcaataaga
    13261 atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca aggtgtataa
    13321 tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt acaatttttt
    13381 ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca aaaaaaattt
    13441 aacccacata ataagcttga tataaacata gctaacgtta aacctaagca aatcagaaaa
    13501 tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa cattatgata
    13561 gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag tttatatagc
    13621 ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt aaaaaacgta
    13681 gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa aaacaaaaca
    13741 attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg tagggattca
    13801 ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct
    13861 tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccatc
    13921 aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat ggtaggctca
    13981 caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    14041 aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa acgtcttttt
    14101 caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag ttcagccaat
    14161 tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc agggcagctt
    14221 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    14281 acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg tcaaaacgct
    14341 gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga tattgttctc
    14401 tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa
    14461 aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta cgcaattctt
    14521 tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct ctttcttcct
    14581 catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga ttacggatac
    14641 cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca gatggatcaa
    14701 ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt actaatggcg
    14761 aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga acgagacgtt
    14821 tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac
    14881 cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta tcatagaaaa
    14941 catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca ttgatcccaa
    15001 ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa
    15061 cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat ttaaagtcaa
    15121 tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag cctagacttt
    15181 ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt gcaaaatcaa
    15241 aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct aatttaaata
    15301 agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg cgcatatctt
    15361 tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca tccggattat
    15421 agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg aagatcatcc
    15481 aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca aatccttcaa
    15541 tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg ctcactggaa
    15601 cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt
    15661 tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt accatggtat
    15721 ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct tgcgactgat
    15781 aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa
    15841 cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt
    15901 cgtatacacg tttgatccca gccatattca atccctctga aagatagtct ttaatctcaa
    15961 gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt cgttccggat
    16021 gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca gtcagcttca
    16081 taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct cccatcagac
    16141 gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg
    16201 catatgttag gttttctaac atgagatctc tattttttat aaaataacaa aaaaataaga
    16261 acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt tattatactg
    16321 tactatcctt tattagtgtt tttatt
//
LOCUS       DACCWV010000059        16344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCWV010000059 DACCWV010000000
VERSION     DACCWV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.059"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..933)
                     /locus_tag="IVW84_001596"
     CDS             complement(13..933)
                     /locus_tag="IVW84_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7917792.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            1120..1611
                     /locus_tag="IVW84_001597"
     CDS             1120..1611
                     /locus_tag="IVW84_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7917793.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(1635..4010)
                     /locus_tag="IVW84_001598"
     CDS             complement(1635..4010)
                     /locus_tag="IVW84_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7917794.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            4336..4962
                     /locus_tag="IVW84_001599"
     CDS             4336..4962
                     /locus_tag="IVW84_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7917795.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            4985..5689
                     /locus_tag="IVW84_001600"
     CDS             4985..5689
                     /locus_tag="IVW84_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7917796.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            5881..6714
                     /locus_tag="IVW84_001601"
     CDS             5881..6714
                     /locus_tag="IVW84_001601"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7917797.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            6911..8839
                     /locus_tag="IVW84_001602"
     CDS             6911..8839
                     /locus_tag="IVW84_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7917798.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            8977..9432
                     /locus_tag="IVW84_001603"
     CDS             8977..9432
                     /locus_tag="IVW84_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7917799.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            9902..10171
                     /locus_tag="IVW84_001604"
     CDS             9902..10171
                     /locus_tag="IVW84_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917800.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            10571..10825
                     /locus_tag="IVW84_001605"
     CDS             10571..10825
                     /locus_tag="IVW84_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7917801.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(10914..11960)
                     /gene="holA"
                     /locus_tag="IVW84_001606"
     CDS             complement(10914..11960)
                     /gene="holA"
                     /locus_tag="IVW84_001606"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7917802.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(11998..14271)
                     /locus_tag="IVW84_001607"
     CDS             complement(11998..14271)
                     /locus_tag="IVW84_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7917803.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(14264..14803)
                     /locus_tag="IVW84_001608"
     CDS             complement(14264..14803)
                     /locus_tag="IVW84_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7917804.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(14939..15157)
                     /locus_tag="IVW84_001609"
     CDS             complement(14939..15157)
                     /locus_tag="IVW84_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7917805.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(15154..15399)
                     /locus_tag="IVW84_001610"
     CDS             complement(15154..15399)
                     /locus_tag="IVW84_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7917806.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(15515..16243)
                     /locus_tag="IVW84_001611"
     CDS             complement(15515..16243)
                     /locus_tag="IVW84_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7917807.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5000 a         3174 c         2901 g         5269 t
ORIGIN      
        1 aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata cgccagttgt
       61 tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat gagttggtaa
      121 aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat catacatttc
      181 gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc caccgacaga
      241 cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta cctcttctgg
      301 cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg catcaattac
      361 ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt taaagaagaa
      421 gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga ctgtttttcc
      481 agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa agcccatttt
      541 taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat tcatcaaaat
      601 attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac atacaacatt
      661 cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata caaacacagg
      721 gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc attcatataa
      781 aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat tggctggaat
      841 caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa ccactatttt
      901 ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca cctctcccct
      961 actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat acttcgaact
     1021 caaatgagaa gaattttagc aaacaggtaa gaatctgccc taatcctatg ataagataga
     1081 tgtatggtaa atatttatat gtatatcagg agggttccta tggcaaaaga tgctagcttc
     1141 gacattgtat cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca aacaacaaaa
     1201 gaattgtcca atcgctttga cttcaaggat tctacagtag aaatcaaatt agagaacagc
     1261 aatctagttg tattgagcga tgatgatttc aaaatcgaac agatcaaaga tgtattattt
     1321 ggtaagctga tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga atcagaacac
     1381 gctctaggtg gcaaagctcg acaaactgcc gatttggtca acggaatcga tcgtgaaaac
     1441 gcgaagaaaa tcactacagc tatcaaaaat gaaaaactga aagtaaaagc acaaatccaa
     1501 gaagatcaga ttagagtgac aggtaagaac cgtgatgatc tacaagctgt gattgcttta
     1561 ttacgtaaac ttgacttacc aatcgagttg caattcacta actatcgtta atcaaaaaac
     1621 gaggctgacg tctgtcattc ctcgtttttt tgatttactg cttcttttga taaccaaaaa
     1681 cgctgtattc cttcttccaa ttccccaaca cttcgtactt ctcttgctgt ttgccaatgt
     1741 tgtgggaata aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa tacaacgatt
     1801 ccttgatccg ccatgccacg aatcactcga ccaccagctt gcaagacttt gttcattcca
     1861 ggaagctgat aagcgtaagc aaatccaagg tgttccttct catcataata tgatttgatc
     1921 agttcctgct catgattcat ctgcggcaaa cctacaccta cgatcatact gccgatcaat
     1981 cggtcccctc tcaaatcgat tccttctgaa aaaatcccgc caagtacgca aaatccaatc
     2041 aatgtttcag cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc ttcctcattc
     2101 atgtccgttt cttggattag cacattgctg tcgggatatt gctgtctgaa tgcttccacc
     2161 gcaagatcta aatattgata agaaggaaag aaaacgaaat aattcccgac ctttccatca
     2221 gcaaaagcat gaatcgtctc tactaatttt gccagactgt gttccctttt tcgataagtc
     2281 gtctctaggt aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg actcggcagt
     2341 ttgtaagcca gacttttttg tccccccagt gtttcctgat aataagagag tggtgaaaaa
     2401 ctagcagaaa aaagaatact gctttttcct ttatccaacg actgctcaag aaacaagctt
     2461 ggatcaatac aaaactcttt tactatcaga tcgcgatacg ttttttcgac tgttgtttcg
     2521 tagtgatcat cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa ataataagga
     2581 agcaattgtt cttgtgatgg gtgctcagga aattctgcca gccattcttt aattttttcg
     2641 cttaattgat aacctgcctt tactaaagat tcagccggtg ctttttgatg atgataatcc
     2701 caatgatctt cttttgctat ttctcggatc gaatcaaatt cttttaatag tttgttgaaa
     2761 cggcgatgaa gttttttgaa ttcctttggt atagcttcct tacttcgttt cgttttttca
     2821 taagatagtt ctgccgaata catctctcgg gaacgattca ccaaattatg tgcttcgtca
     2881 atcaaaaaca gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa ataaacagtt
     2941 ggatcaaata aataattgta atccccaatg attacgtcac accagatact gacatctaat
     3001 gatagttcga aaggacaaag tgtatgcttt cttgcataag tttcgatgac ttcacgagtg
     3061 atctgattct catgattcaa aagatcccat aaaccttcat taatccgatt gtaataacca
     3121 ttggcatatg ggcactgatc tggattacag gtcgtctcat ctaaaaagca gatcttgtct
     3181 ttcgccgtca aagtgacact ttttacttca gctcctgtat cttttaatgc tgtcatagca
     3241 tcttcagcta cttgtctagt tattgtttta gccgttagat aaaagatctt ttctccctct
     3301 tcttccccta cagctttcaa agcggggaat aacgtagaga ttgtttttcc tgttccggta
     3361 ggtgcttcta caaacaattt ctgtttcgta cggatcgttt tataagcagc taccgctagt
     3421 tcacgctgac ctttcctata ctcatcatgg gggaatttca aagccatcaa agaggcattg
     3481 cgtacagtgc gccagttttc ttgaaaaaca agccattctt tataatcttc agtcagcttt
     3541 ttaaaaaaag tttctaactg ctctcttgtc tgatgttcaa cctttctcgt gatcacttct
     3601 tctgccgtct ggaagtaagt caattgcaga tttatttctt gtaaatcatg ttcttgacta
     3661 tagatatagg catacaccct tgcttgatga aagaaaagtt ccacttgttc tggttctaaa
     3721 tcttcaaatc gtggttcgga ggttttgatt tcatctatat aataaatacc gtctcttttg
     3781 aaaataccgt ctgcacggcc ttcaacgatc aggtaatatt catctaattg aactgttgtt
     3841 tttaaaaaga cttctttttc atagttttct cccgcatctt tttggagctt gcggtggatt
     3901 ctcgctcctt ctaatgcagt atggttactt tttttacgat tgtcgatact tccacgtcgc
     3961 aaaataaatt ctactaagtg ccgcacagat atttttgact gtttgatcat ttccaccacc
     4021 tctttctctc taagattacc tgaaaaaacc tagtttagca aaaagagctc tctttccaaa
     4081 cataaaaaaa caccctgctg attaggcagg gatataggat atagaatttg gatttttgga
     4141 tgaatctaat gtaacaagga catattaaaa tgctatgaaa acttactttc cttctcttta
     4201 gaatatccaa atacttttca tatagtctga taattcagat aagtaaagag aaataagtac
     4261 ctgtttttgt ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag ttttggctat
     4321 caaggaggaa tgcacatgca tatcgaaaga ttaaaaacag gaatgattga agaaaactgt
     4381 tatttggtct acaacgaaga agcacttttg atcattgacc ctggagaaga tgcggaacga
     4441 atcaaacaag aaatcaaaaa aacaaaccaa cagccggtag cgattttgct tacccatact
     4501 cactacgatc atatcggtgc agtggaacct ttacgcgcgt attatcaaat cccagtttat
     4561 gtcagcccac tagagcaaaa atggctaggt gatccgattt tgaatctttc tggactcggt
     4621 agacatgatg atattgctga tgttattgtc tcgcctgcag aatatgaatt tgaaatgaaa
     4681 acttatactt taggaaacat gtcttttcgc gtcgtcccta ccccagggca ttctatagga
     4741 agcgtcagct ttatttttga tgattttgtc gtttctggcg acgccctgtt tagaggaagt
     4801 atcggtagaa ccgatctgta tacagggaat ttagaacaat tacttcatag tattcggacc
     4861 cagctgtttg tattacctga tgaatttgca gtctatccag gacatggcga tgcaacgacg
     4921 attgaacagg aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt aggaggagaa
     4981 taaaatgaca gaaactacac tttatatcgt acgacacgga aaaacgatgt tcaatacaat
     5041 tgaacgagta caaggctggt gtgatacacc cctcacaaag caaggacaag aaggcattca
     5101 ttatttaggt cgcgggttaa aaaatgtcga cttttcattt gcttattcta gtgacagtgg
     5161 tcgtgcaatc gaaactgccc gtattattct aggagaacat tctaagggaa aagaaattcc
     5221 ttattttatt gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag gtggatatga
     5281 catggaaatg tggggtgtga tccctcgtgt attgaatttt aaaacatatg atgaaatgtt
     5341 taccacagaa gtttcttttg aacaaattgc taatgctgta tacaacttag ataccgctgg
     5401 gtgggcggaa acttatgaaa cattgcgaga ccgtgtatgg acaggatttg aagatatcgc
     5461 ccatcactgc gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg gtttgacgat
     5521 cgcctttttg cttaatctga tcgatcctaa acagcctgtt cgtgctgggc ttttaaatgg
     5581 cagcgtaaca aaggtcactt atgcaaacgg tcgattttct atccacggca tcaatgatac
     5641 gtcttatatc gataacggga aaacacatag tcgcgactcg tttctataat ggggatattt
     5701 taatttttac cagagaatca taaaaggcaa agtctgaacg agggctttgt cttctttttt
     5761 ctcttcgtgt acttatctgc tttatttctc aagtttttct taatattaaa aaacagattg
     5821 ggtttctcaa aaaataacgg tatactataa gatgtatctt aattgggaag gaaggaattt
     5881 atgttttttg caaatattat caaagcagtg attctcggta ttattgaagg aataacggaa
     5941 tggctgccca tcagtagtac tggtcatttg atcctagcag atgaattcat caaactggat
     6001 gccagttcac aatttatgtc gatgttcaat gtcgttatcc aattaggggc gatcttggct
     6061 gtcgtcgttc tttattttca caagttaaac ccattttctc caagtaaatc aacggtagaa
     6121 aaaaaagata cgtggacact ttggtttaaa gtgatcgttg cttgcatacc agccatcatc
     6181 attggtttgc ctttggatga ttggttagaa gcaaatttcc acaagttctt accagttgcc
     6241 attgttttga tcgtttacgg tattgccttt attattgtcg aaaaacgcaa caaaaacaaa
     6301 acacctcgtt ggacttctct gaatgacttt acgtaccaag cagctcttct tgttgggtta
     6361 ttccaagtct tggcacttgt tcccggaaca tcccgttctg gcgcaacgat cctaggatct
     6421 atcttgattg gtgcttcgcg ttttgtcgct acagagtttt catttttctt agggattcct
     6481 gtcatgtttg gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg gaattcattc
     6541 aaaatggaag aaactgttgt tcttcttgta ggaacattcg tatcttttgt ggtttccgtc
     6601 ttagctatca aattcttact aagctatttg aagaaaaacg actttaccgc ttttggctgg
     6661 tatcgtatta tccttggtat cattttgatt ggttattggt ttattgcaat gtagaaaaaa
     6721 ccagaacact tctcttattt gagaaatgtt ctggtttttt attttcttta ataataatca
     6781 ttataaataa taaaaacaac atcaagctta atcaccaaat gataaccatt atcgttctca
     6841 tttagctgtt attattatca attacatttt tattatgcta tccttgtttt gacaaggagg
     6901 tctaataacg atgactaatc agaaaaaagc ttttgcagca acaatcggct gccttttatt
     6961 tatgatcttc ggttttatct tcgaccgatt ctctcttacc atcaccccac ttttttatat
     7021 cagtgccatt atcatcggtg gatataaaca aacatcggaa ggcttgaaag aattgtggaa
     7081 tgatcggaca ctgaatgtgg atttattgat ggctttagct gctattggtg cctgtttgat
     7141 cggtaattac tttgaaggag caatgttgac attcatcttt tgtttaagcg gtgcattaga
     7201 ggaatacaca actaataaaa gtcaaaaaga aattactgct ttgatgaatc ttcaaccaca
     7261 aaaagcccaa ttattaaaag aaaatggaca aatgcaagaa gtagatgttt ctcaactaaa
     7321 ggtggataac ttggtatttg tcgctaaagg agcagctgtt ccgatagatg gctttttaga
     7381 aagcacacag gcaacgatcg atgagtctgc attaagcggt gaatctgtgc ctgtagaaaa
     7441 atatttaggc gatgagatgt atgcaggaac gctgaatcaa ggaaatccca tcacgattcg
     7501 agtaacaaaa acgagttccg aaactgtctt tgctaagatt atccaattag tagaagaagc
     7561 gcagaatacg ccaactcaaa cagcttcatt tatcgaacga atcgaaaata attatgtaaa
     7621 attgattttg cttgctgttc ctctaatgat ccttctcccc cactttttcc taggttggag
     7681 ttgggatgaa agcttttatc gaggaatggt attattagtt gtggcttctc cttgtgcgct
     7741 tgtcgcatct gctacaccag cttctctagc tgctctttca aattctgcaa aaaatgggat
     7801 tttaatcaaa ggcggtatcc atttagaaaa gctttctgaa ttaaaagcga ttgcctttga
     7861 taaaacagga acgctcacta aaggaaaacc agttgtaact gacagctttt tctttgatga
     7921 aaaggatttg gcacaacaac ttcttgttgc tatggaacaa aaaacgacgc atccacttgc
     7981 tcatgcgatt ttgcaatatt tcgattgcac aatacctgaa gaaatccaat acttaccaat
     8041 agaagaaatt actggttttg gattacaaac tacctattta ggtgcgcaat ggaaagttgg
     8101 caagcacgca tatgatcctg agacgatgat aatcagcaaa gagattgcgg aaatgatcga
     8161 acgtttggaa aatcaaggca aaaccgtgat ttatctttct aaagatcagc agttaattgc
     8221 tgttttaggt ctgctagata ttccaaaagc aaatacccaa caagtaattt cctactttaa
     8281 atcccaaaac atccatacaa gtatgataac cggcgaccat tctggcacag caaaggccat
     8341 tgctgaacaa gtagggattg aacattacta tgcaagctgt actccggaag aaaaaacaca
     8401 actagtcaaa aaagagaatg aactttacca agtaaatgcg atggttggtg atggagtcaa
     8461 tgatgcgcct gctttagcag ccgcttcttt aggtgtcgct atggggcaag ggacagatgc
     8521 cgctatggat atcgcagaca tcgtcttaat gaaaagcgac ttaaataaat ttgtctacag
     8581 tcatcggcta tctttaaaaa tgcggcggat catcaaacaa aatattattt tttcgattct
     8641 tgttattctc ttgttgattg cttctaactt cctgcaattt ttaaatctac ctcttggagt
     8701 tgtaggacac gaaggaagca caatattggt tattctgaat ggcttgcgtt tactaagacc
     8761 tttacctgat ctttctgatt cttcaaaaaa atgtaagtct tgtccactat acaaagcgcg
     8821 gctgtcttct gtcaaataag caaagtgttt ttttatgatt ttgtttattt tatgaaaaaa
     8881 acagcttatg aataggtctt gaaggatgaa ttcctacttt tgtaactttc taaaaacatg
     8941 ttatgattgt ttcgaagaaa gtgaggaata tatttcatgg aaagttggct ttttttagga
     9001 attattttac ttgtagcaat tttagctaaa aatcaaagtc tgatgatagc ggcagctttt
     9061 gttttgatta tgaagctcat accagcctcc cgttcctttt tcccttggct tcaaggcaaa
     9121 ggaatcaata ttggggtgac gatcatcaca gcggctattc taatccctat cgcaacagga
     9181 gaaatcggtc tacgtgatct attagaagcc tttaaatccc ctattggctg ggtagctgtt
     9241 ttttgcggtg ggctagtggc tgtcttatct tccaagggtg taggattgat tgcttctgat
     9301 ccacaaatca ctgttgcact tgttttcggc acgattttag gtgttgtctt cttaagagga
     9361 atcgccgccg gaccggtcat tgcttctggt atcacttact gtatcttaca agtatttgct
     9421 ttaggaaaat agaacaaatt ttgtctattt taggagattc ttaacacccc caatttactg
     9481 atccgtgata tactgacaac gaggtgatac aatgttcgaa aaattaaagt atttcatagg
     9541 aacaatcggc tggtttcttc tagcggtact tttgattctg ggcttagctt atctattatt
     9601 tcctgatttg atacaaaatg tgtttgatac tgctttttga ttgacctatt aaaacgatat
     9661 aggggatgct cctgcgatgc aggggctttt tttaatcaat gttttgtaat aatttaggcc
     9721 gtgccaatga gacttcctat agaattcgat gctctttttt ctttttggct cttctgctct
     9781 tttttgttat actagattac agtggacaaa agaaagaatg atcgcatcca cttcaacgaa
     9841 tatcaaaatg gaagaattag tgcctgtttt caggttactt tgaaaagaaa ggaagacgct
     9901 tgtggaaact tttgaaggtc ttttaattga tacgtacgtt ttacaagaac agccaatgat
     9961 gctgactggg atcaacttgc agcttgagga ccgtgtgatc aatggcctgc ttaaagatcg
    10021 ctatttggca aaatatatct tacgtttgcc cctaaataaa tatgtgctgg aagtaagtgg
    10081 caaatggaac cgtcggaacc agttggtcat aaaaaaaatg ttgataaaaa atatcgatga
    10141 atacattaaa ataatcggta caccagaata gcctttactg atcaaaggat actggttata
    10201 aaataactgc agtttcttaa tagaaaaaca aagaacaaca gggaatcgta tcccttttca
    10261 aaaaaaactt tgcaaacggt tctctttgtt atataataac ttagtaccaa acattcccat
    10321 tcaaactttg tttaaataga taaataaaaa tctagtttgg tttgacgcta ttgcctccca
    10381 caatagcgtc tttttgattt ttttcagctt gtactatccg tttttactga aaaaaaatcc
    10441 taataaaaga ctgttaagga aaaaaacttg tcatgttcat attgcaaagc acgctgcctt
    10501 taggtataat aatgaatgtt gaattatgcg gtaacacgcg taagaactta agttatcgga
    10561 ggtgaaacta atgccaaaca ttgaatctgc aatcaaacgt gttcgtacaa gcgaaaacgc
    10621 taatgtgaaa aattcatctc aaacaagcgc tatgcgtact gcaatcaaaa aatttgaaga
    10681 tgctgtagct tcaggtgctg acaacgtaga tgcgttatat aaagaagctg ttaaagctat
    10741 tgatatggct gaatcaaaag gtctaatcca caaaaacaaa gctaaccgcg acaaatctcg
    10801 tttaagcaaa aaaatcgcta aataaaagaa tcggcttcgg ccgatttttt tatttgtcaa
    10861 aaaaaggaca aaaacaaacg accttcccgc atgctttgcc ctcgtctttc ttcttatcga
    10921 tttcttaaca actgccctgt gcgcaaaacg aatagttgaa aacttaattc tttttcgatt
    10981 tgtcccgttt tgatttgata atccatctca actaattgat caaacaactg taccagcaaa
    11041 tgttcatcga atttttttac ttcctgcatg gcaagtttta cacgatatgg atggattttc
    11101 aatgtttcag caatatttgc ctgctgataa ccaatcttta caagaaattt tgtctgtaaa
    11161 aataaacgta attgagaaag caaaatcgca ttaattttga ttgtttcttc cccttgcata
    11221 actaaatctt cgtataaacg caaagcttgt tccgtcttcc cttttaaaat atattgggtc
    11281 atgtcaaaaa tattatgttc taacgtttta ggaactaatt cttctacttc tcttaccgtg
    11341 atttgctgtt gattttgtcc ataaaggacc aatttctgta actctctcat cattttggat
    11401 aaatctaagt cggtcaacct caagaataaa tcaattgcac ttcgttccat tttgattgag
    11461 gaattctcta aggtattcat cagatattga cgtacttctt tttcattcaa ctgttggaca
    11521 ttgatgataa ttgctgtttt tttcaattgt ttcgttactt ttttccgttc atccaacttt
    11581 tcatatggcg caaagaatac taatacagtc gattctaatg gttctttcaa ataagccgtc
    11641 aataaatcag tattttgttc gattccattg tttacttttt cacctgttaa aaaataagga
    11701 ttctctacga atactaaccg ttgatctccg aaaaacggca acgtctctgc ttcagctact
    11761 acctcatcta aacttgcatt ttccatgtca aaacttaaaa aatttaagtc atcttcgccc
    11821 tctaattgga tttttttgac aatttctgtc cgaacttgat cttggagaaa cttttctgtt
    11881 cctaaaatca agtaacaaga tgcaagtttt tctttacgta ttttttgtaa ctctgcttgt
    11941 atactcaaat tactcaccac tttcaaaaat tatcctacca tgagcagatg agaattacta
    12001 gtctatcatt gtttccacta gcccatgagg atgaaaaact ggatgccagc gataggtgat
    12061 cattccttgt tgatccgttc gccatgtttt tatctgattt tcttctagtg tctccaatac
    12121 ttcgctgtgc gggtgaccat aacgattatt tacgccgcag gatataatcc catgttttgc
    12181 ttcaatatgg gaaagaaaat ctgtgttact tgacgtacgg ctaccgtgat gacctaattt
    12241 gactacatcg gctttcagat taggataaat caaaagcaat tgtttttcca ttgcttgatt
    12301 caaatctcct aaaaataaaa agcgcctatt gttatattcc atccatagac caatcgaatc
    12361 ttcatttctt ccctctccct tttcaaaagg agtcaataca taaagatcat aataaccgct
    12421 gatcctgtcc ccatgtttga tttcaatgat ctcagtacca tttttttgag cttcagataa
    12481 atgcctttgt aatggctcat ttcgtccagc gccccaaccg atataaattt ttttgatagt
    12541 aaaatgctga gataacgtgg gtaattctcc cacatgatcc gtatcgtcgt gtgtcaagat
    12601 gagtttatcg attacactta tcccctgacc ttttagatat ggaacgatat ttctttcagc
    12661 aggaggacgt atcatacctt gcgcccactt ttctctttca aagtgtaact gacctccagt
    12721 atcgatcaaa atcgtttctt ttcgaaaagg actttgcaaa aagatactgt ctccttgacc
    12781 gacatcgata aaagcgatcg ttgaagaaaa gcgtaaaaat ggcaaagtca aaaaaataaa
    12841 agaaaaaaga ccaacataaa acaagtgttt cacaggttgt tctttcaacc tatccataca
    12901 aatcagtaat ccgaaaatat acatcccaga tagcacaagg ggcaaacgtc ctataatgag
    12961 agacgaccct gaagaaaaat cgattattcg ctcaaataag attaatccct cttctatcag
    13021 caaaagtaaa gtttccggca gccagcgatt gaagagtgtg agaaataaca caccaggaag
    13081 aaaaagaaac ttgaataccg gtgcaatcag taatgtaaaa agactaccta aaataggcca
    13141 ctcataaaaa tgccatacaa tcaacggcat cgtgatcata gtaaacagga cgtgagaggc
    13201 cgtttttttc aaccaatctc catcaaaaga aatcaggata aacaaaaaac tcatgaaaaa
    13261 actaagctgt ccacccgcat gaaaaagaaa tcgaggatca aatagtaaaa gaagccaaaa
    13321 gacaactgca aataagtcca tggtggaata atgcttattc agcaatttga ctagcaatcg
    13381 caataaaaac agcaaactag cacgcaaaac agaaatagaa cttcctgcaa tagaaacata
    13441 acagatagtt agtagactca acggaataat actgacgtgt agtggccatc cgatacgtcg
    13501 aaataaataa tacagccacc ctaaataaaa ttggatgtgt agacccgata gactgaacag
    13561 atgcagcaaa ccactctccc taaaaatttc ttctggttct ctgaatcctg catctttata
    13621 gccccataat aaagaattaa tatatacact tgttttttgc ggaaattcag attggacaaa
    13681 ggagataaaa gatcctcttt ttctctgaag ccagtttcgc cagtgatttt gcatggtgat
    13741 ctgttcaatc ttttgaattt ggaaccgtcc agaaattcta tggttcctga agtaagcttc
    13801 ttggtcaaaa ttataaagat tccgcttttt tgctggagag atgaactcgc cttcagcagt
    13861 caattctatt ccctttcctc ggaagttaag ccatgttttc aattctgctt ctgtgtttac
    13921 ttgataagaa agctggagct tttgtttatt ctctgataac cttccttcca ttgtcaaaaa
    13981 attaccattt gcacgaatcg tatctgtaaa tacagtaatt tgcgcagatg ttgttttttc
    14041 ctctaaaaat gaatactctt gtagcctata agaaatggat accgttatgc tacacaaaat
    14101 acacaagtac gcgataaaac gtccttcttt tagcgaaaac cataaaaaaa ttccggcaag
    14161 aagcaaacga taaccccaat tgtcagtgta atacaacaaa cagataatca tcgacgtaat
    14221 tgccggaaaa atcaaccgat ttcgaacatg cctttctaaa acgctagaca tgttttcctg
    14281 ttaattcatt ttgaagtttt gaaaaatgct gtgagtctaa agcaacttgg tgtacagata
    14341 cacctaattg ttcgatcaac gcttgggcat aaggatcatt tcgatagtca tggagataat
    14401 tgatcccttt cacaccagct tgcaacaaag cctttgtaca ggccaaacat ggaaaatgtg
    14461 tgacatagat ttctgaacct tccgtcggga taccaagttt tgcacactgt aggagtgcat
    14521 tcatctctgc atggatcgtt cgcaagcagt gtccatccac gacgtaacac ccttcatcta
    14581 tgcagtggac atctccactg actgctccat tatagccgcc agcaatgatc cttttttctc
    14641 gaacgagtgt cgctcctact tctaaacgtg tacacgtact acgtaaagat aacagtactg
    14701 cctgagccat aaagtactgg tcccaaggaa tgcgttcatt atgtgactta tcagtaactt
    14761 tttctttatt cgtatcgcat gcttttgaag atttttctaa catgttttat attcctccgt
    14821 tttttcaagt tgattaaaaa gtactcattg cttaaacaaa atattcttta aatataacga
    14881 atcatcctgt ctgacaaaag atcctaccct tcactgccga aatgaagtcc gtcatccatt
    14941 agcccattat aaaaattctt cgaatccatt tgttcaagaa attccgtata aaaatcgata
    15001 cttcacaatc atggcgtttt gctacttctg tgagaacaga gatatattct ttgatcaact
    15061 tgttattccg atatttttgt tttgtctcat cgactgctgg tggcgtgata aagaaagcat
    15121 gatcagaact atagtctttt ttcagctgaa ctatcatcca ctgcagattt tttggtacgt
    15181 ttctttagaa agttgtttat gctctgctag atcattcgtc ccaatgagaa taaatacttt
    15241 atctgctgta tagctaagta catctttggc aaagcgttct cgtagagctt gactgttatt
    15301 gccgctgaca gccgtattca taatatgaat cccagatatt ttattttgga gatagacatt
    15361 cagcattggt ttttttaatg tttctttgcg aacaatcaag ctatttccta tccaaaaata
    15421 ctacctcctc gtcctgccaa gctcttagaa ttggattatt cttagtatcc attagcgtcc
    15481 atttattgct cgtgccagtc gattttgaat cattctattt aattgtaacg aagtttttta
    15541 gtttttcaac tgtcttttca ccgataccag aaatctcctg caggtcttcc actttctgga
    15601 aggatccgtt ttcttctcta aatcgaatga tttcttgcgc tttgactgat ccgatcccag
    15661 gaatctgctg aagttctgat ggagtggctg tattgatatt tattttggga ggctcggaat
    15721 ctgctgtagg ctgttttcct tccgaggatg ccaactgatc aagatgttct ggcagctctt
    15781 cacctacagc agggatatat aggacttgct gatcttctat cttcgcagaa aagttgattc
    15841 gatcgctatc cgcttcttct aataatccac ctgcacgctt tagtacatca tagacccgtt
    15901 cttctgaaga aaaggaatag ataccaggct ccttcacact tcctttgata tcggtgtaca
    15961 tgacctttgg acgagcatcc tcttctacat cgtttccttc agctgatgga tcttctccac
    16021 tggaactaaa cgaagaaagt atttcttttg cagcagtctc ttctgtttct ctataaaata
    16081 gcaaatttga taataaaaaa ccacttgcga tacaaaagat aagaactatc atagacatcc
    16141 aaatgatcaa tttgggtgaa gctagtattt tcttgctgat tttttctatc attgttcttt
    16201 tttcaaagaa tgaccagtaa aaatcattca ttactccttt catttaaaat tgaagaaaca
    16261 gtgagctctg gcaaaatcgt cccttcaaca tctaattacg tcaatttttt cttttgtttt
    16321 tatttttatc gtgaagattt ttga
//
LOCUS       DACCWV010000060        15985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCWV010000060 DACCWV010000000
VERSION     DACCWV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.060"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            226..894
                     /locus_tag="IVW84_001612"
     CDS             226..894
                     /locus_tag="IVW84_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7917808.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="IVW84_001613"
     CDS             complement(1094..1324)
                     /locus_tag="IVW84_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917809.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="IVW84_001614"
     CDS             complement(1451..2833)
                     /locus_tag="IVW84_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7917810.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="IVW84_001615"
     CDS             complement(2989..4176)
                     /locus_tag="IVW84_001615"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7917811.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="IVW84_001616"
     CDS             complement(4183..5214)
                     /locus_tag="IVW84_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7917812.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="IVW84_001617"
     CDS             complement(5260..6684)
                     /locus_tag="IVW84_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7917813.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="IVW84_001618"
     CDS             6859..7764
                     /locus_tag="IVW84_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7917814.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="IVW84_001619"
     CDS             7855..8532
                     /locus_tag="IVW84_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7917815.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8813..9709
                     /locus_tag="IVW84_001620"
     CDS             8813..9709
                     /locus_tag="IVW84_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7917816.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="IVW84_001621"
     CDS             complement(9764..11134)
                     /locus_tag="IVW84_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7917817.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="IVW84_001622"
     CDS             11372..12118
                     /locus_tag="IVW84_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7917818.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="IVW84_001623"
     CDS             12115..12954
                     /locus_tag="IVW84_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7917819.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="IVW84_001624"
     CDS             12955..13905
                     /locus_tag="IVW84_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7917820.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="IVW84_001625"
     CDS             14046..14798
                     /locus_tag="IVW84_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917821.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="IVW84_001626"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="IVW84_001626"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7917822.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5190 a         2995 c         3221 g         4579 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa catta
//
LOCUS       DACCWV010000061        15897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCWV010000061 DACCWV010000000
VERSION     DACCWV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.061"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            189..1214
                     /locus_tag="IVW84_001627"
     CDS             189..1214
                     /locus_tag="IVW84_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7917823.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(1809..5462)
                     /gene="rpoC"
                     /locus_tag="IVW84_001628"
     CDS             complement(1809..5462)
                     /gene="rpoC"
                     /locus_tag="IVW84_001628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7917824.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(5541..9158)
                     /gene="rpoB"
                     /locus_tag="IVW84_001629"
     CDS             complement(5541..9158)
                     /gene="rpoB"
                     /locus_tag="IVW84_001629"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7917825.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            9886..10854
                     /locus_tag="IVW84_001630"
     CDS             9886..10854
                     /locus_tag="IVW84_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7917826.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10872..11777
                     /locus_tag="IVW84_001631"
     CDS             10872..11777
                     /locus_tag="IVW84_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917827.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11774..12580
                     /locus_tag="IVW84_001632"
     CDS             11774..12580
                     /locus_tag="IVW84_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917828.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12737..13705
                     /locus_tag="IVW84_001633"
     CDS             12737..13705
                     /locus_tag="IVW84_001633"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7917829.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(14022..14546)
                     /locus_tag="IVW84_001634"
     CDS             complement(14022..14546)
                     /locus_tag="IVW84_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7917830.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14833..>15897
                     /gene="scm"
                     /locus_tag="IVW84_001635"
     CDS             14833..>15897
                     /gene="scm"
                     /locus_tag="IVW84_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7917831.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4611 a         3383 c         3063 g         4840 t
ORIGIN      
        1 aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc
       61 ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga
      121 caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc
      181 atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa
      241 ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac
      301 ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa
      361 atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc
      421 cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact
      481 tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc
      541 aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa acacttcttt
      601 ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca
      661 cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac
      721 tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat
      781 ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta
      841 atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt
      901 taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga
      961 gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag
     1021 gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc
     1081 cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat
     1141 accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac
     1201 aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact
     1261 ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt
     1321 cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt
     1381 tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt
     1441 ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa
     1501 acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata
     1561 atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca
     1621 aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata
     1681 ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac
     1741 aaattatttt agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt
     1801 taaaatatct attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata
     1861 tctgaaatac tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga
     1921 taacgttgca taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa
     1981 ccaagaagag gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct
     2041 tggaatgatg cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt
     2101 aagacaggtc gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct
     2161 ttaaagtctg cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt
     2221 acacgaactt tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct
     2281 accccttgca tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat
     2341 acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca
     2401 cggtggatca tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta
     2461 cgtgtatctg tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag
     2521 atatcgatga catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct
     2581 tcaaagattt cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct
     2641 gtatggaatg tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc
     2701 gtaccaactg cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa
     2761 tgtttgcaga caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg
     2821 acacctgcat caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg
     2881 atcaattcat ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga
     2941 tcttctaatg attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg
     3001 tcagtaccgc aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga
     3061 gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg
     3121 tgagttgaga taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc
     3181 aattccatga ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta
     3241 aagttggaaa tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct
     3301 tccaaacctt ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg
     3361 acacgttcat aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt
     3421 tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca
     3481 gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg
     3541 tcaagcatct tagaagtttc ttttactttg aatcgtttga agacttctgc gatgatgttt
     3601 cctaagtttt tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata
     3661 tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt
     3721 ggttcattca gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact
     3781 gttgtgatca tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta
     3841 gggttcacac cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct
     3901 tcgttttgat ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag
     3961 tagtagttac ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg
     4021 ttcaagatgt tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc
     4081 aatggaacgt ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca
     4141 agtggatgca aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca
     4201 agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct
     4261 aaaacatccc atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt
     4321 gaagctagtt cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc
     4381 atttcttttg gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa
     4441 cgacctgagt agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct
     4501 ttcaacatat gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg
     4561 cgacgaccgt tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga
     4621 acgatgattc caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt
     4681 acacgacggt aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc
     4741 attgggcgta aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga
     4801 tttcctgaag cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt
     4861 tgtccttgtg cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca
     4921 ttatcaagta attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat
     4981 tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta
     5041 tttcctggtt cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg
     5101 ctcatgtcta atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa
     5161 acaggagctg ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact
     5221 tccactccgc atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag
     5281 gcacattccc agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca
     5341 ggttttaagg tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt
     5401 ctgatttttt cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc
     5461 aaaaggggtt ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc
     5521 cttgcagctc atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg
     5581 acatcgtctt tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt
     5641 tgctgttcgg caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc
     5701 atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt
     5761 gattgcaatt ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt
     5821 tcacccttga cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat
     5881 gtcaagattt cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt
     5941 tcaccaaaac gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat
     6001 gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc
     6061 atgtacatca caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac
     6121 agaacagttt tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca
     6181 tcacttgcac catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata
     6241 cccaagtgta attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc
     6301 agcatgatat cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata
     6361 cgggatacaa cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta
     6421 cgtttttgaa cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca
     6481 gcttcacgag taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt
     6541 aatgatgtat cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct
     6601 tctgcagata attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct
     6661 tggacttctg caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc
     6721 ccgacattag ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct
     6781 gattcatatt cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc
     6841 atgatgatcg catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt
     6901 tggcctagag ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt
     6961 tcgactttct cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa
     7021 cgacggaatt ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt
     7081 tcagaagcat caacgtattc aacgacacca tcatgtttac ataacaaagc agctcctgag
     7141 tcatgcgctg atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac
     7201 ggcactgctt gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc
     7261 aagaaaggaa tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag
     7321 tctactttat caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct
     7381 tgagcaaatg ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct
     7441 tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca
     7501 cgacgataag gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta
     7561 ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg
     7621 gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct
     7681 aaggcgaaaa gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac
     7741 tgtgacaact gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg
     7801 atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca
     7861 cgttccatac gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg
     7921 atacgacgat ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc
     7981 aagaaatagc tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct
     8041 ggatagccgt ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg
     8101 atgacttgga cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact
     8161 gaatttaggc cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact
     8221 gttccttttt caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc
     8281 agattcaata gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt
     8341 gctaagtcgt aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct
     8401 gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt
     8461 cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg
     8521 tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg
     8581 gttaaaggaa tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt
     8641 tccatttcta gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg
     8701 tttttgtcga cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt
     8761 actcgctctg ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg
     8821 aagaatactt cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca
     8881 tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt
     8941 tctttcaatt cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc
     9001 aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt
     9061 tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt
     9121 tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt
     9181 cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta
     9241 acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg
     9301 aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata
     9361 tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact acacttattt
     9421 tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt
     9481 tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg
     9541 ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat
     9601 cgcaccaaca caactgaata acttttactt tagctacttt agataggttt gtattcatca
     9661 tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg
     9721 cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa
     9781 gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt
     9841 gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata
     9901 atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat
     9961 tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac
    10021 tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa
    10081 ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt
    10141 atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca
    10201 gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac
    10261 ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg
    10321 acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc
    10381 aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg
    10441 gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca
    10501 aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct
    10561 actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa
    10621 acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga
    10681 atcgactacg aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc
    10741 aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa
    10801 gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca
    10861 ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct
    10921 tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact
    10981 tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg
    11041 gctatcatcc attgacagct tgtcttctag gctttttagg gagtgcggca gcagggcttg
    11101 tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca
    11161 tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg
    11221 gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac
    11281 tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta
    11341 cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg
    11401 ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc
    11461 tttctggctc cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa
    11521 cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac
    11581 tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt
    11641 tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag
    11701 tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag
    11761 gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa
    11821 aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga
    11881 cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc
    11941 cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa
    12001 atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac
    12061 agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg
    12121 cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg
    12181 gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca
    12241 acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga tcttattaga
    12301 tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa
    12361 gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat
    12421 ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca
    12481 agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg
    12541 cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa
    12601 aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat
    12661 caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg
    12721 aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc
    12781 agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg
    12841 gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta
    12901 tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt
    12961 tattcctgat ttgaaagtac aaacgatgat gacttctgaa tctacgatga aagatgccaa
    13021 actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca
    13081 agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag
    13141 tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc
    13201 cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt
    13261 caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga
    13321 tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc
    13381 tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca
    13441 agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct
    13501 ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac
    13561 cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca
    13621 tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag
    13681 tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata
    13741 cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt
    13801 tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt
    13861 aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag
    13921 gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac
    13981 ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac
    14041 aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg
    14101 agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg
    14161 gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct
    14221 agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa
    14281 tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa
    14341 attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa
    14401 gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc
    14461 tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc
    14521 tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt
    14581 tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta
    14641 acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat
    14701 tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt
    14761 caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa
    14821 ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat
    14881 atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat
    14941 atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta
    15001 tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa
    15061 cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata
    15121 aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca
    15181 agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct
    15241 acagtcaatg gatccaggct tgatcactta actccagatg atttttataa agatcgtgct
    15301 gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat
    15361 cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga catgaacaat
    15421 cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca
    15481 gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta
    15541 tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat
    15601 cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat
    15661 gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct
    15721 actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat
    15781 gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg
    15841 acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DACCWV010000062        15880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCWV010000062 DACCWV010000000
VERSION     DACCWV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.062"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            108..860
                     /locus_tag="IVW84_001636"
     CDS             108..860
                     /locus_tag="IVW84_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7917832.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            857..1771
                     /gene="pfkB"
                     /locus_tag="IVW84_001637"
     CDS             857..1771
                     /gene="pfkB"
                     /locus_tag="IVW84_001637"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7917833.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1789..3720
                     /locus_tag="IVW84_001638"
     CDS             1789..3720
                     /locus_tag="IVW84_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7917834.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3831..5390)
                     /locus_tag="IVW84_001639"
     CDS             complement(3831..5390)
                     /locus_tag="IVW84_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7917835.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5545..7779
                     /gene="pcrA"
                     /locus_tag="IVW84_001640"
     CDS             5545..7779
                     /gene="pcrA"
                     /locus_tag="IVW84_001640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7917836.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7796..9832
                     /gene="ligA"
                     /locus_tag="IVW84_001641"
     CDS             7796..9832
                     /gene="ligA"
                     /locus_tag="IVW84_001641"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7917837.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9938..10243
                     /gene="gatC"
                     /locus_tag="IVW84_001642"
     CDS             9938..10243
                     /gene="gatC"
                     /locus_tag="IVW84_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7917838.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10243..11715
                     /gene="gatA"
                     /locus_tag="IVW84_001643"
     CDS             10243..11715
                     /gene="gatA"
                     /locus_tag="IVW84_001643"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7917839.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11712..13142
                     /gene="gatB"
                     /locus_tag="IVW84_001644"
     CDS             11712..13142
                     /gene="gatB"
                     /locus_tag="IVW84_001644"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7917840.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13152..14207
                     /locus_tag="IVW84_001645"
     CDS             13152..14207
                     /locus_tag="IVW84_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7917841.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14318..15691
                     /gene="rlmD"
                     /locus_tag="IVW84_001646"
     CDS             14318..15691
                     /gene="rlmD"
                     /locus_tag="IVW84_001646"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7917842.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15694..15822
                     /locus_tag="IVW84_001647"
     CDS             15694..15822
                     /locus_tag="IVW84_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7917843.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5397 a         2902 c         3480 g         4101 t
ORIGIN      
        1 ttttattttt ttttgactga tttataattt ttatgaacgt ttttgactga tttatattgc
       61 gagtagaaaa aaaatatgct attatttgta ttgaggtgat cgtgaaaatg cttacagagg
      121 aacgtagaca aaaaatccta gacttgcttg aacagcagaa aatcgtgaaa tcacaagatc
      181 ttgtaaacct ttttaatgct tctgaatcaa cgataagacg tgatttacaa gagctggaga
      241 atagtgggtt tctagaaaga gttcatggcg gagctaagaa agagcaatta ttgaattttg
      301 agcagaatat gagtgaaaaa tcactcaaaa acgttcacga aaaacagcaa atcgccaaat
      361 cggcagctga aaccattaat gacgaagatg tcatttattt agacgctggc tctactacgt
      421 tagaaatgat tccttttctg caaggaaaac agatcacagt agtgacaaat tccgttcaac
      481 atgcggcaaa tttagttgat ttggagcttg ctaccattat tttaggtgga acgataaagc
      541 tggcaactaa tgctgtttta ggatctaatg cgatagaaca actaaaaaat tttcatttta
      601 ataaagcatt tatgggaatg aacggtgccc atcttgaaca aggattcacg actccggacc
      661 cagaagaggc agctgttaaa cgtttagcca tgaaaaattc cgaagagaca tttgtattga
      721 ttgatcatac caaattcaac cagttgtcct tcacctctgt tgcacctttg gaagcagcca
      781 cgatcataac cgagcgttgt ccattagaat tcgttaatga attccgtgaa aagacaacaa
      841 ttaaggaggc aatacaatga tttacacagt aacacttaat ccatcaattg attatatcgt
      901 acgcgtagat gggttaaaac taggcgattt gaaccgaatg aaggaagact tcaagttacc
      961 tggaggaaaa gggatcaatg tttcccgtat tttgaaacgg atcgattctc aatctacagc
     1021 actagggttt ttaggagggt tcactggcga atttatttcg aagtggcttg aaaaagaaca
     1081 aatccagtct ttgttcacaa ccgtaaaaga agatacaaga atcaatatca aactaaaatc
     1141 agatactgaa actgaaatta acggtttggg cccatctatt tcagatgaag aaatcgatga
     1201 gttgaagcaa gcattaggaa cagtaggaca aggagatatc gtcgtattgt caggaagcgc
     1261 gccggctagc ctgcgaaaag gattttatca agaactgatt gatatcattc gagaaaaagg
     1321 tgcagatttc gtgattgata caacaggtga tgacctaaaa aatgcacttg ttaagaaacc
     1381 attgttgatc aaaccaaaca atcacgaatt agctgagctt tacaatacaa ccttccaatc
     1441 tgtagaagaa atcattccgt atggtaaaaa attgctagct gaaggggcac agaatgtgct
     1501 gatctcaatg gctggagacg gtgctttgct cttcacggaa caaggaactt ataaaacgaa
     1561 tgtattagtt cgcccattga aaaattcagt aggtgcaggg gattcaatga ttgccggctt
     1621 tattggggct tacagccgta cgaaagatcc aatcgaagcc tttaaatggg gagttgcttg
     1681 cggaagcgca acagcttttt ctgatgactt ggcagctcgc gaattgattg atgagttgct
     1741 gccagaagtt gaaatcacaa aaattgatta gtttcaagga ggataatcat gaatatcaag
     1801 gatttaatgg tcaaagaagc gatgatcatg gatttgcaag cgactgataa aaaaggcgca
     1861 atcgatgaaa tggtccaaaa aatgtttgat gccggccgga tttccgatat cgaaacatat
     1921 aaagaaggaa ttctagcccg cgaagcacaa acttctacag gtcttggtga tggaatcgcc
     1981 atgcctcacg cgaaaaacag tgcagttaaa gaagcaacag ttttattcgc aaaaagccaa
     2041 aaaggagtag attatgaagc cttagacgga cagccaactt atctgttttt catgatcgca
     2101 gctccagaag gcgcaaacga cacacatttg caagcattag cagcactatc tcgattgctg
     2161 attgatgcag atttcgtagg aaaattaaaa gaagcaaata caccagaaga agtccaagac
     2221 ttgttccaaa cagctgaaga gcaaaaagaa gcagaagaaa aagcagaaca agaaacacaa
     2281 gctgcagaag cttctgctca tccagaaaga aaattcgttg ttgccgttac agcttgtcca
     2341 acagggatcg ctcacactta catggcagaa gatgctctga agaaaaaagc gaaagaaatg
     2401 ggtgtggaga tcaaagtcga aacgaacggt tcagaaggaa tcaaaaaccg attgactgct
     2461 gaagacatcg cccgtgcaga aggtgtaatc gttgcagcag acaaaaaagt agagatgaat
     2521 cgctttgacg gaaaagaact gatcaatcgt cctgtcagtg atggtatccg taaaccagaa
     2581 gagctgatca atctagccct tagtggtact gcaccagtat tccatggtga tggaaaagaa
     2641 agcagttcag aagaaggaaa tgcggatggt actatcggac aaagaattta caaagatttg
     2701 atgaacggcg tatcacacat gttacctttc gttattggtg gaggaatcgc tattgcgctt
     2761 tctttcatga tcgaccaatt tattggcgtg ccacatgatc aattatcaaa tctagggaat
     2821 tacaatcaag ctgctagctg gttcaaccaa atcggacaag ctgcatttgg tttcatgctg
     2881 ccggtattag caggatttat tgcttcaagt atcggtgacc gccctggttt gatcgttgga
     2941 tttgctgctg gtgcattagc taatacaggc ggtgcaggat tcttaggtgc actgattggt
     3001 ggattcttag ctggttatgt catcgtattt ttacgtaaat tattcaagaa cttaccaaaa
     3061 tcattggatg gaattaaaac gatcctcttt tatccagtgt ttggcttact gattactggt
     3121 ttcttgatgt tgctggtcaa tgtccctatg aaagcaatca atgatgcatt gaacggtttc
     3181 ttgacaagta tgagtggttc gaatgctgct ttacttggtg cattattagc tggaatgatg
     3241 gcggcagatt taggcggtcc aatcaacaaa gccgcttatg tgtttggtac agcaacttta
     3301 gctacgactg ttgcaactgg cggaagtgtc gtgatggctt ctgttatggc tggaggaatg
     3361 gtacctccat tggcaatttt cgttgctaca cgtttgttta agaataaatt cagcaaaaca
     3421 gatcaagatg ctggtttgac gaacatcgtg atgggactat cgtttgtcac ggaaggatcg
     3481 attccatttg cagcagctga cccgattcgt gccattccaa gttttattat tggttctgct
     3541 ttaactggcg gattagtcgg agctttcggg atcaaattac tggctcctca tggcgggatt
     3601 ttcgttgtat tcttactgag tcacccggtc atgtatttgg tgtttattgc tatcggagca
     3661 gtcgtatctg gtgtgattta tggtttattg agaaaagctc ctgcagcagc agaagcatag
     3721 aaataaaaaa gaagaggctg gtttttccca gtctcttctt tttttataga atcatataga
     3781 atcaagtagt gtagatgaat agttgttgta aaatagtttt tccatgttcg ttaaaatgat
     3841 tgcttcaaat ttgtgataga agatttagcc tcgatttcct gcttgattct tggcaagttt
     3901 gcttcatcag tcaagataac caaaatatct cctacattca tgatcgtatc accgtgagtc
     3961 aagatttcct gatttcctct gcgtatagag attagcagca tatttttagg ccaattgaag
     4021 tctcgtacca ttaatccgtc taaagaactc tctgccgtta ctggaaaatc aaaagttgtc
     4081 cttgcacctt ttagtgaggc ggtctgagta tttccaagta gacgttccaa taatgattcg
     4141 taaattggat taccgccgag aaggtcattg acgatataag caacaagagc agccaatcct
     4201 aaaggcatca gatgattaag agagccaacc atttctgtca ctaaaataat tgctgtcagc
     4261 ggtgcttttc caatcgctgt gaaataacca gccatggcaa taacgacaaa atccttgatg
     4321 tagattggat ccatcccgaa atcaaccata atgtttccat aaaagctacc gattaaagcc
     4381 cccaatgtca atatcggaag gaaaatcccc cctggaagac caccaccgta agaaatcatg
     4441 gaaaaaacga atcgaagaac aaacaaaccg ataatcataa tgattggcaa ggaagcatgt
     4501 ccaaaagcta aaacgatttg attgcctccg cctagagtac taggccataa ataaccaatt
     4561 ggcaaaatca gcaagaaagg aacgaacccg tgaaaataag ctggtaaagg caatcggttg
     4621 taaaattttg gcaaagccag caagaccttc tgataaataa aacctaagat gcctagaagt
     4681 actcctaaga tcactaagga accataatat ttcaacggca aagaaggaag gtcacctaga
     4741 tataaaacag gtctcaagcc gaaaaagtaa agagagacga agttagcagt gattgctgaa
     4801 gcaaatgatg tcagccaaac aagtggagaa aaggagtgat ggatttcttc taaaacaaag
     4861 agtaatcctg cgataggagc attgaatgct gctgcaagtc cgctacttgc accgctagaa
     4921 attaaaatct tttcttctga agatggactc cgaaacaatt gtccagctcc ttgtccaatg
     4981 gatgcgccaa gctgtataga aggtccttct ctacctagaa aaaggcccgc accaactgaa
     5041 agtacaccgc caatgaactt tttccataaa acagagaacc agttcattga taattccccg
     5101 cgaagctgtc cctctacttg tggaatcccg cttcccttga tatccggttc atttttgacc
     5161 agataaccca cgaaaaatgc tgcaaggatg cagcaaatag ctaggccaat taagtaaatc
     5221 ggctgttccc gactcatttc atagatactt cgaactaaat cgcttagctt ttcgatagat
     5281 aatcgaaaca ggctaacgac gattcctgtc agcagtccaa caaatattcc ttttagaata
     5341 aaatatatct gagtcttatc taatttcttg atttttcctt ttttttccat tcaaacgcct
     5401 cgctttcttt taatttagcg cgaaatcaac gaattgacaa ggaaaaatcc ataaaaagaa
     5461 acaaatgatt ttgaactagc tagtttaatg aaagccaaga aaatgctata attaacaaga
     5521 ttgataaaag aaaggatgtt gttaatgtca tccaaagctg aattattaaa cgggatgaac
     5581 ccccgtcaga aagaagctgt tcttcacact gacggaccat tactgctaat ggcaggtgca
     5641 ggaagtggaa agacgagagt gctgacacac cgcattgcct atttgattga ggaaaaagaa
     5701 gtcaatcctt ggaacatcct agcgattact tttaccaata aggctgcaaa agaaatgaaa
     5761 gaacgtgtca atgcgattct cgcatctgga ggcgaagatg tctgggtatc gacttttcac
     5821 tcgatgtgtg tgcgtatttt aagaagagat gttgatttta tcgggtataa ccggaacttt
     5881 acaatcatcg attcttctga acagctgacc ttgatgaaac ggatactaaa agaactgaac
     5941 attgatccga aaaaatatga tcctcgaagt atattaggaa caatctctca agcaaaaaac
     6001 agcctgcaga cccctcagga ttttgcaaaa atgcaaggca gttattacga agagattgcg
     6061 gcaaaatgtt atgctgctta ccaaaaagag cttcaatata atcaatgtat ggactttgac
     6121 gacctgatca tgaatacgat tcgattattt gaagaacatc ctgattcact cacatattat
     6181 caaaacaaat tccattatat tcatgtagat gagtaccaag atacaaatca cgcacagtat
     6241 acgctagtca atttattggc aggtagattt aggaacctgt gcgtcgtggg agatgcagat
     6301 caaagtattt acggctggcg cggtgcagat atgcagaaca tcttggattt tgaaaaagat
     6361 tatccagatg ctgcagtaat tttgctagaa caaaactatc gctccacgaa aaacatcctt
     6421 agcgcagcaa accaagtcat tgaaaacaat agcaatcgaa aaccaaaaaa cttgtggaca
     6481 gaaaacaaag aaggaaacaa gatcacttac taccgtgcag ataatgaacg agacgaaacc
     6541 cgttttatcg tagatcggat gcaagaagag attcgaagca atcatcggaa ttacggcgac
     6601 tttgccatct tgtaccggac gaacgcacaa tctcgtgtaa tggaagaaac tctgttgaaa
     6661 gcaaatattc cttataaaat ggttggaggg cacaagttct acgatcgtaa agaaatcaaa
     6721 gatattttag cgtatttgaa tgtgctggca aatcctcaag attcaatcag ttttgagcgt
     6781 atcgttaatt cgcccaaacg aggcatcgga ccaggatcta tcgaaaaact acgcagtttt
     6841 gcttctcttc atgaatggcc actgcttgaa gcagcgcaaa atgtggatct ggcaaatatc
     6901 ggtggaaaag caggacagca attaggtgca tttggagaga tgatccaaga agtcactcag
     6961 atgatcccat acttgacagt gacagaattg acgaaagagg ttttggatcg tagcggatat
     7021 ctagaagact taaaaatcca aaatacatta gaagcacaag cacgcatcga aaacttggag
     7081 gaatttttaa cggtcacaca agaattcgac aaacaattcg aacagcaaaa tgaagaagac
     7141 gctgatgcac cagaagaaaa actaacggtc ttcttaaatg atctggcact tgtttccgat
     7201 attgacaatt tagaagaaga tgcttcccaa gtgacattga tgactttgca tgcagccaaa
     7261 ggattagaat tcccagttgt attcctgatt ggtctagaag agggggtttt tcctttatct
     7321 cgagcattga tggaagaaag cgaactggaa gaagaacgcc gtctcgcata tgtggggatc
     7381 acgcgagcag aagaagcgtt gtatttaaca aatgctttct ccagaacatt atatggacgg
     7441 acgcaatata accgaccttc tcgttttgta gaagaaatcg atcaggagtt actggaaatc
     7501 gaaggcatgc gtcctacgcc taaaaaaaca cctgtattcg ctaaaaaaac agcatatagc
     7561 tataaacagc cagagacagc ggttgttccc tctaaaagtg ctactggagg ggaaaataac
     7621 agctggaaac ctggagataa agtcaaacac aaaaaatggg gtctaggaac agtcgtccga
     7681 gtaagcggga cgtcgaagga tctagaacta gatgtggctt tcccaagtca aggagtaaaa
     7741 cgtcttttgg ctgcctttgc gccaattgaa aaagcttagt agtggagggt aaatgatgga
     7801 acatgaaatg acgattgacg aagctgctaa acgagcagaa gaacttcgta caagactgaa
     7861 ccaatggtct cgcgaatact acgtagaaga taaaccaaca gtggaagact atgtttatga
     7921 taaagaatac gcagaattag tagcaattga ggaacaatat cctgatttga tcacgtctga
     7981 ttctcccact caacgagtag gcggaaaagt attagaaggt tttgaaaaag tcactcatga
     8041 tattccgctt tacagtttga acgacgtttt cagcaaagaa gagctgatag cttttgatca
     8101 acgtgtacaa aaagcagtgg gacgtgtagt tgactattgc tgtgaactaa aaatagatgg
     8161 actttccgtt tctttgcgtt atgaagatgg aaatttcgtg agaggtgcga ctcgcgggga
     8221 tggaactgta ggagaaaaca tcactgaaaa cctaaaaact gtccggtcgg tcccaatcaa
     8281 attaaaagaa ccaatgaaca tcgaagtaag aggcgaatgt ttcatgccaa aacggtcatt
     8341 tgtccaattg aaccaagaca gagaagcaga gggaaaagat atctttgcga atcctcggaa
     8401 cgccgcagct ggttctttgc gtcagttaga ttctaaaatc acagcgaaaa gaaatctgga
     8461 cactttttta tacacagtag ctgatttcgg tcctatgcaa gcaaaaaccc agtatgatgc
     8521 gctagaagag ctagaaaaaa ttggattcca cacgaatcgg gaaaaacgtc tatgtcattc
     8581 gattgatgaa gtgtggtcat acattgaaga atatcatgac aaacgtgtgg atttgcccta
     8641 tgaaatcgat ggaatcgtca tcaaagtgaa tgaattctcg ctacaagatc aacttggttt
     8701 tactgtcaaa gcaccgagat gggcagcagc ttacaagttt ccgccagaag aagttgaaac
     8761 gctaatcgaa aacatcgaat ggacagtcgg tcgaacaggg gtagtaacgc caacagctat
     8821 catgacacca gtccgagtag caggaacgac agtaagccgt gccagcctgc ataacggtga
     8881 ttatattaag ttgaaggata tccgcttgaa agatacagtt ctgatttata aagcgggaga
     8941 catcatccca gaagtttcgc aagtcgtgtt agataaacga cctaaagaca gcgaagaata
     9001 ccaattgcct acacattgtc cagtttgtgg aagtgaactt gtccatttgg atgaagaagt
     9061 agccttacgg tgcatcaatc ctaaatgccc tgctcaaatg aaagaaggtt tgaaccactt
     9121 tgtttcaaga aatgcgatga atatcgatgg gttggggccg cgcgtactag aacagatgta
     9181 tgataaaaaa ctagtagcag atgttgctga tctttacaaa ttgacagaag aagagttatt
     9241 gacgttggac aaaataaaag aaaaatcagc aaataatatt ttgacagcaa tcgacaacag
     9301 caaagataat tcggtcgagc ggttgatttt cgggttagga atccgacatg tcggagcgaa
     9361 agcagcaaaa attttagcag aacatttcgg tgatctagaa acattgagta aaagtgacta
     9421 tgagtcgatt attgcgcttg atacgatcgg tgatatcatt gccgacagtg tagtcactta
     9481 tttcagtaac gaagaagtcc atgaattgat gaatgaatta aaacaagcag gcgtcaattt
     9541 cgaatacaaa gggcttcgaa atgcccaatt gcaagaagtc gagtctcctt ttaaagaaaa
     9601 aacagtagtc cttactggta aactaacacg atttactcgt gaagaagcaa aagagacgat
     9661 cgaaaatctt ggcgggaaag tgactggtag cgtatcgaaa aaaacggata tcgtggtagc
     9721 aggagaagat gctggcagca agctgaccaa agcacaagaa ttaggaatcg aagtttggac
     9781 agaagatcaa atggcagatg cattggctaa aagtcgctca gtagaagaat gaaaaatgag
     9841 ttttttcaga caaatttcaa taaacaattt cagacacggc actcttgtgt taaaatagag
     9901 agcaaagttt ttaactcgtt tagaaagaag gggtagtatg gcgattagtg aagaacaagt
     9961 gaaacacgtt gccaaattag caaaacttgc gttcgcagat gaagaacttg caagttttac
    10021 cgaccaatta ggtaaaatca tcgatatggt tgaaatgctg gaagaagtag atacagaagg
    10081 tgttccattt acgtcgaatg tggttgaaac agtaaacgtg atgcgacaag atcgggcgca
    10141 agctggatgg aaccgcgatg aattattgaa aaatgtgccg gaaacagaag acggctttat
    10201 caaagtaccc gcaatcatcg ataatgggga ggctggcgca taatgacaga attacacaat
    10261 cgttctctag aagaactgca tcatatgctt gtatcgaaag aaatcactgt acaagatttg
    10321 acaaaagcaa catttgaacg aatcaaagaa acagagcctg aggtcgaagc attcattaca
    10381 ttgaacgaag aaaaagcatt ggaacaggca aaagctttag atgaaaaagg gattgatgaa
    10441 agcaacgtcc ttgctggtat tccaattggt atcaaggata atatcgtaac aaaagatatc
    10501 ttgacaaccg ctgcatctaa gatcctttac aattttgaac caatttatga tgcaacggtc
    10561 atggataaaa tctatcaagc agatatgatc cctgttggta agctaaacat ggatgaattt
    10621 gctatgggtg gaagtacaga aacttcttat ttcaagaaaa caaaaaatgc ttgggaccca
    10681 acaaaagtac ctggtggctc ttctggtggt tctgcagcag cagttgcagc tggacaaatt
    10741 ccagtttctt taggtagtga tactggggga agtatccgcc aaccagcagc ttttaacggc
    10801 atcgtaggga tgaaacctac ttatggccgt gtctcacgtt ttggtttgat tgcctttgct
    10861 tcatctcttg accaaatcgg accaatgacc cgtacggtga aagataatgc attagcattg
    10921 acagctatca gcggttatga cgaaaaagac ggtacttctt caggcgtttc agttcctgat
    10981 tttgcagagg gactgacagg cgacatcaaa ggaatgaaga ttgctttacc taaagaatac
    11041 ttaggcgaag gtgtggcccc tggcgtaaaa gaagctgtat tgaaagcagc agaaacattc
    11101 cgttcattag gtgctgttgt ggaagaagtc agcttgcctc attcaaaata cggtgtggct
    11161 gtttattata tcattgcctc atcagaagca agttccaact tgcaacgatt cgatggtatc
    11221 cgctacggtt atcgctcaga aaatgtgaaa tcattagatg atgtatatgt caactcacgt
    11281 tcagaaggat tcggtgaaga agtaaaacgc cgtatcatgt taggtacatt ctccttaagt
    11341 gctggttatt atgacgctta cttcaaaaaa gcgggacaag tccgtacatt gatcaaacga
    11401 gattttgaaa aagtctttgc agattatgac ttgatcatcg ggccatcttc accaacagtt
    11461 gcattcggaa tcggtgagaa cattaacgat ccaatcacaa tgtacatgag tgacatctta
    11521 acgatccctg tcaacttggc tggacttcca ggtatgtctg taccggcagg tttatcagaa
    11581 ggattaccag ttggattgca gatcatcggt aattattttg atgaaaaaac gatgtatcaa
    11641 gcggcatatg cgtttgaaca agcaacagaa ttccataaac aacaaccagc tattttagga
    11701 gggaaagacg aatgaatttt gaaacagtca ttggactaga agtccacgtc gagttaaaaa
    11761 ccaactcaaa aattttctct ccagcgccag ctcattttgg tgcagaacca aacagcaata
    11821 caaacgtgat cgactggggt tatccaggcg tgctgccagt aatgaataaa gctgcgctgg
    11881 aattcgggat gaaagcagct ttggcattaa actgtgaaat ctctaaagat actcattttg
    11941 accggaaaaa ttacttttac ccagataatc caaaagcata tcaaatttcc caatttgatc
    12001 aaccaatcgg tcatgacgga tggatcgaaa tcgaagtaga aggtaaaaag aaaaaaatcc
    12061 ggatcgaacg tgtccattta gaagaagatg ctggaaaaaa catgcatggt attggcggtt
    12121 attcttacgt tgatttgaac cgacaaggca cgcctttgat cgaaatcgtt tctgaagctg
    12181 atatgcgttc accagaagaa gcatacgctt atctagaagc attgcgatca attatccaat
    12241 ttacagaagt cagcgatgtg aaaatggaag aaggctctat gcgctgtgat gccaatattt
    12301 ctcttcgccc ttatggacaa gaagagttcg gtacgaaagc agaattgaaa aacttgaact
    12361 ctttatcttt cgtgaaaaaa ggtttagctt ttgaagaaaa acgtcaagca aaagttcttc
    12421 tttcaggagg agaaatccaa caagaaacac gccgttttga cgaaacaacg aataaaacag
    12481 ttttgatgcg tgtcaaagaa gggtctagtg attatcgtta cttcccagag cctgatatcc
    12541 cgaaattcgt gatcgatgac gaatggattc aaaaagtaaa agctagtttg cctgagatgc
    12601 cagcttctcg tcgcgaacgc tatatccgtg agtttggact tccagagtat gacgcaatgg
    12661 tattgacact tacaaaagaa atgtctgatt tttttgaagc tgttttggaa aatggtgcag
    12721 atgccaagca agcatctaac tggttgatgg gtgaagtttc tgcttacttg aacagtgaga
    12781 aactggaatt agctgagaca aaattgacac ctgagaactt agcaggaatg attcgtttga
    12841 tcgccgatgg aacgatcagc tcaaaaatcg ccaaaaaagt cttccgtgaa ttgatcacaa
    12901 atggcggaga agcccaagaa gtagttgaaa aaaatggttg ggtgcaatta tctgatccga
    12961 acaaactact gccaatcatt aatgaaattt tggataacaa ccaacaatct gtcgatgatt
    13021 tcaaaaatgg aaaagatcgt gcggttggct tcctagtcgg tcaaatcatg aaagcaacta
    13081 aaggacaagc aaatcctggt gtcgtgaata agctacttaa agatgaatta gcgaaacgat
    13141 agaggctgaa aatgaaaaaa gcacgcgtaa tttataaccc cacctcggga aaagaattat
    13201 tgaaaaaaaa tttggcggat atcctacagg cattagaaga agcaggattt gaagccagcg
    13261 cctatgcaac tacaccagaa ccagattcag ccaagaatga agcatgtaga gtcgcagaac
    13321 taggttttga tctagtagtg gcagctggtg gcgatggcac aatcaacgaa gtggttaatg
    13381 gaatcgctcc gcttgaacac cgtcctaaaa tggccatcat cccagctggt acaacgaacg
    13441 actatgctcg agcattaaaa attcctagag ataatattaa agcagcagca gaagtgataa
    13501 aaaaacacca aaccgtaaaa atggacatcg gcctttctga taacagttac ttcatcaata
    13561 tcgctgcagg tggctatttg acagaattga cttatgaagt tccatcagaa ctaaagagta
    13621 tttttggtta tcttgcttat cttgcgaaag gagcagagat gctccctcga gtaaagccaa
    13681 tcaaaatgcg cttgaagtat gatgaagggg aatatatcgg aaatgcttcg atgtttttcc
    13741 tagggctgac gaactctgtt ggaggattcg aacagatcgc gccagatgcc aaattggatg
    13801 atgggaagtt ttctttaatc attgtgaaga cagcaaatat ctttgagatc ttgcatattg
    13861 cagcactcat gctgaatggc ggtcgacatg tcgatgatcc aagaataatc tataccaaaa
    13921 caagcagttt gttcgttgaa acagaagatg atatcaatcg accagtaatg atcaacttag
    13981 atggtgaata cggcggggaa gcaccgatgc atttccaaaa tcttcatcaa cacattgagt
    14041 tctttgccaa taccgatcag attcctgatg aagcaatcac aggtacagat gaagaagaat
    14101 tagaggttgt cagcaaagaa ttcgtaaaag aagtcgaacg tttaacggat gaagatatcg
    14161 acggtgacgg aaaaatagca gacgaaacac ctaatcaaaa gaattaatca aaagccggtg
    14221 ctgaacgtag taaggtcagt gccggtttta gttgtgtaat tagctgattt atagtagagt
    14281 tcagtaggat tgaaaaatag gcttaaagga gcaaaatatg acagaacagc aaatagtaaa
    14341 gaaaaacgaa cgatatacag tcgaaatcgt cgacttgagt tatgaaggaa tgggggtagc
    14401 aaaaatcgat ggttatccat tatttatcga aaatgcactc cctggagaag aagtagaaat
    14461 actagtagta aaagcaggca ataagtttgg atatggaaaa gtcgaaacat ttattacaca
    14521 atcacctgat cgtcagcctg tagaaaacaa tgcattactt cgcataggta tcgcaccttt
    14581 agcccatttg aagtatgaac aacagcttgt cttcaaacaa aaacaagtag agcatgtaat
    14641 ggcgaagatt gctaagatgc cagaagttcc tgtacttcca acggtcggaa tggcagaacc
    14701 attcggctac cgtaataaag cacagatccc tgttcgccgt atcgatggac agttagccac
    14761 cggtttttac aaaaagaata gccatgatct agtccctatc gaagattttt atatccaaga
    14821 tcctgtcatc gatcaagcaa tcaaagtagt cagagatatc ttgcaacggt tccaagtacg
    14881 aggatataat gaagaaaaaa atgaaggaca gcttcgtcac atcattatcc gtcgtggtca
    14941 ttattcccat gaaatgatgg tcgttttagt tactcgtaaa gaaaaattct ttaaatccaa
    15001 ggaaattgct gaagctattc atgaagaatt gccagaagtg gtttcggtca tccaaaatat
    15061 caatgaagaa aaaacaaatg tgattttagg agacaaagaa aaagttttat atggcagaga
    15121 ctatatcgaa gatcagctat taggaaaaac ctatcgaatc tcagcaaagt cattttatca
    15181 agtaaatact caacaagcag agtatctgta ccaaacagcc atcgagtttg ccgagttaga
    15241 taaaaaagac attgtgatcg acgcttattc aggaataggt acgattggct tgtctttagc
    15301 agacagagtg gataaggtgt atgggatgga agttattcca gaagcaattg aagatgcaca
    15361 gttcaatgct ttaaataata aaatcgaaaa tgttcattat gaagtcggaa aagcagaaaa
    15421 agttatgaaa aaatggcaag aaaaagggat caaaccatct gtgatcgtag ttgaccctcc
    15481 acgaaaagga ttagatcatc attttattga atcagcaatc gaaatgtcac cagaacgaat
    15541 cgtctatatc tcatgtaatc cggcaacttt tgcaagagat gctaagttat ttgcagaagc
    15601 aggttatgcc gtgaagaaag ttcagcctgt agatcttttc ccgcaaaccc atcatatcga
    15661 attagtcgct tcattcgtta aaggagaata aaaatggacc aaaaagaaaa aatgaaagaa
    15721 ttgatcaaaa agaaacaagg tggaggccga tccaaacaaa tggaaacacc taagaatgat
    15781 cgtaaaaaca tgagaaaagg ccctaaaatc tttaataaat agacgtgcta aagaaagact
    15841 aagaagtgtt aacttttgtt attgtttctt agtcttttta
//
LOCUS       DACCWV010000063        15821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCWV010000063 DACCWV010000000
VERSION     DACCWV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.063"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            93..614
                     /locus_tag="IVW84_001648"
     CDS             93..614
                     /locus_tag="IVW84_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7917844.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(1313..1878)
                     /locus_tag="IVW84_001649"
                     /pseudo
     CDS             complement(1313..1878)
                     /locus_tag="IVW84_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2057..2713)
                     /locus_tag="IVW84_001650"
     CDS             complement(2057..2713)
                     /locus_tag="IVW84_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917845.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(3281..4216)
                     /gene="whiA"
                     /locus_tag="IVW84_001651"
     CDS             complement(3281..4216)
                     /gene="whiA"
                     /locus_tag="IVW84_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7917846.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(4250..5248)
                     /locus_tag="IVW84_001652"
     CDS             complement(4250..5248)
                     /locus_tag="IVW84_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7917847.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(5245..6129)
                     /gene="rapZ"
                     /locus_tag="IVW84_001653"
     CDS             complement(5245..6129)
                     /gene="rapZ"
                     /locus_tag="IVW84_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7917848.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(6264..6995)
                     /locus_tag="IVW84_001654"
     CDS             complement(6264..6995)
                     /locus_tag="IVW84_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7917849.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(6999..8264)
                     /locus_tag="IVW84_001655"
     CDS             complement(6999..8264)
                     /locus_tag="IVW84_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7917850.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(8527..11343)
                     /gene="uvrA"
                     /locus_tag="IVW84_001656"
     CDS             complement(8527..11343)
                     /gene="uvrA"
                     /locus_tag="IVW84_001656"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7917851.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(11353..13347)
                     /gene="uvrB"
                     /locus_tag="IVW84_001657"
     CDS             complement(11353..13347)
                     /gene="uvrB"
                     /locus_tag="IVW84_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7917852.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13606..15108
                     /locus_tag="IVW84_001658"
     CDS             13606..15108
                     /locus_tag="IVW84_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7917853.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            15110..>15821
                     /locus_tag="IVW84_001659"
     CDS             15110..>15821
                     /locus_tag="IVW84_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7917854.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKD"
BASE COUNT         4643 a         3396 c         2674 g         5108 t
ORIGIN      
        1 gaattggtca tctaattttt agacaaacag tctaaatcgt tgcctttttt tgtcagacat
       61 gtataaacta taagcgaaag aggtgtttta acatggatat atcagtgatt gatgcaacga
      121 aaacaaatac acaaaaagga atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg
      181 aattcatcaa cctatcttgt ccgtattgcc gacaatggtt tgaagaatct tatgagctat
      241 tagaagaagc agtccaaagc gggcaattgc agcgagtgat caaattgttc gataaagaaa
      301 aagaaagctt gcagcgagga aacgtgatgc accggtatct cacaatcagc gatggtcaaa
      361 aagcgatcaa agagatcaaa caaatattcg atactcaaga cgagtggaaa catctttctc
      421 tccaagaagt agcggatttt gcagtagata aactaaagct gacagaacaa aaagacgaac
      481 agctgagcca agctgtcatc aacgaagctg agcaagcaca tattcgattt gttccgactg
      541 tcatcttggg taaagaaatc tttgatgaat ctatttcaat agaggaatta aaagaattga
      601 tccaaacaaa ataaaaaaat aaggagcgta ggacaaaagt aaagaacact tttgtctcac
      661 gctctaaaca cctataaacg gcggaagcag aagcaactcc ttcttatttc ttggagttaa
      721 acgcttctgt cccagcctcc ttattccgaa taaatggtgt ccaaaagctt gcatctgcca
      781 gattatctca tttcttatga caagctgagc aaattatctc caccaaataa tttattcata
      841 tttttgactt gcgcatacta actaattctt aaattttcat acaaaatttc tcaaattccc
      901 ttaacgagta atatttatgt acggtgattc acactagtat cccaatcact ttttaagtta
      961 tcccgatttc aattctactt caaaaatagt tgcttttatg tatgctgtta gttgattaga
     1021 tggtcgtttg ttgagctagg tcgtttattt taaacaagtt gtttctttag cttataccta
     1081 tctcgtctac caaacgcaag atggcaaaca caaatgcata cgcatctgta tttgccactt
     1141 tgattcgtat aaagctaaca actcctaact cctctcattt aatagttgct ctctttttta
     1201 ttcagataca tttttgctga tcacagccta gtatttcaat ggttttgaac actaaaccaa
     1261 attgttccat tacaacattc actggctgat gaaatggatt aatagatttc gattattcaa
     1321 agcgattttc tattggtgga ttgtgttccc aactaacaag acggattcca aagtcattat
     1381 ataactcttc aagagttaac cctttcctgt tcccaataga tgtatctctc ccacgctcga
     1441 tataaaatat ggagtttcga atgccaagac ctggcaaatc tttccttgtt acagttacac
     1501 tgctactaac ttccccatat aaaatccagc tcctccaaaa tgataatata tttttcgacc
     1561 atcatctaac tcatgtaaca ataattgttg tgccctcttg tcactcgcca aatgcaaacg
     1621 ccacgtgcga taccatgcca tgctacctgc atctgcagaa aatccatcaa ataatgcaac
     1681 aattgtatct tctatcacaa tcctttcccc atctacttca tattctaatc taaaatcaaa
     1741 ctcccctcgg gtaatcgcag gtcttggtgg cttaggaccc aatattattc tacttaaaac
     1801 cgcattcgtt cccagatgaa acacagctat aacaacaata atgattaata ttttgtttct
     1861 atttgataaa tttctcatta ttcgcaaatc acctctacat tctcacatac tagttcgtaa
     1921 tactccttta atatcaaaat atctactgat cgtaataata ttcgctatga attcctacta
     1981 ctcgtataag aaaggccaaa tcatcagctt ttacttgctg atgatttggc ttgtttctat
     2041 ttatcgtaat tgttttctaa tcaccaaaat tgttaacaat cggttcagat atttcccaat
     2101 tcactagcct tatattatat tgatttaaca attcttcttc atttattgta ttttgatcaa
     2161 gcggaccaaa ttgttctaat aattctttgt catgtaaaat atcccttcta gtaatgcctc
     2221 tttccctttc aatgaaagaa gcactaggaa aagtatgatt ataaccattc aacctatctc
     2281 ccatgtaata attagcaggt cctggaaaat aaaatatttg cttattatca ctaacttcta
     2341 acagcaacac taaatcttct ccgctggcta aacgacttgt ccatctacga tatctcccta
     2401 tgccttcgtt cattccaaat ccatcaaatt caactattaa cgtgtcttct atcacatgta
     2461 attcttcatt caattcatac tcaagtctaa aaggaaattc tccatatgta atctctggca
     2521 atggtggtgc tggagttctc caaataaata caataactgt gaatagaaaa aatccaacga
     2581 tcgatataaa tgttatgaga cctgctcgaa gtaaaacccg atataataaa aatgctgtat
     2641 ttcgctttgc tatatctaga aaaataaaaa gtactgcaat cataatgata gtaaaagttg
     2701 taaaaaaacg catttttgcc ctccctttat aaacatcgat tctcaaattc taaaccaaat
     2761 cttggtagtt caagttcagg tataacgtat tccaaccata catctcaatt actatttatg
     2821 aacttatgat tatcactgaa taaatcactc catccccttg ttaaaacttc tacattaatt
     2881 acacctggtt gttttcgact agctaatcca atgaacttat caaccatcct tatccaccaa
     2941 ccacgagaat cttgaccaga agtaagaaga gcagttctca aggatgatcg agcagttcct
     3001 tccagttttt taattaatca ggccagttat agataaatac ttgatcttat ctatatcagg
     3061 acttctatat taagcaaaaa tttatgtaca aaactaaaat cttcattcag ttgtctcccc
     3121 gctttttata cgattgtatt aaaaacaata gaagcctttc caaattatgt tactccagta
     3181 aagatggaat tatagtttga cggaagaaaa gcaagtataa cttttaaaaa aaactgatac
     3241 attcgcatct cctgcttcat gtatcagttt tttctttgga ttaaactgct tcttcacgca
     3301 gtttttccgc aaattcattt attttgcgaa ttcggtgatt aattcccgat tttgatatcg
     3361 cacctgaggg aatcatttcc cctaattctt tcaagctgac ctctggatgt tgaagccgaa
     3421 gttctgcgat ttcctgaagt ttttcaggaa gggcatgtag accgaccttt gcttcgatca
     3481 attcgatgtt ttcgatttgt ttagaagccg catcaatcgt cttgttcaga tttgctgtct
     3541 cacaattgac tagacgattg accgaatttc tcatatcccg tacgattcga acatcttcaa
     3601 atcggagcat tgagttggtt gcaccaatca atgttaagaa atcagcaatt ttttctgctc
     3661 cttttaaata agaaatatag ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat
     3721 aatttaacat atcgcaaatg tcttggttat gctgttcata aatagaaaat atttccaaat
     3781 gataacggct cgtttccgga ttattgactg aacctgaggc cataaaagca ccacgtaagt
     3841 aagaacgcat tttttgcgaa tttcccataa tctcgtccga aacgtgtcca ttgaacatca
     3901 taccatccat gatatcaaga tccatcaaca catcttttgt gccttgcttc aaccggacga
     3961 tatagacatt gttctttttt aatttcattt ttttacgaac gagtagctca cttcgtacct
     4021 gataatgatc tttcaaaaga gaatagatac gtctggcaat tgcagcattt tctgtttgga
     4081 cgttcaaaat aaactgctgg ttcacaatac ctaaagagcc gttcattcgc agcaaagcag
     4141 ctaattccgc tttcgcatgt tctcgatgaa cttccaaacc agttagttct ttcttcacat
     4201 ctgcagcaaa tgacatccta ttctcctcct ttctccaaca ataaattgct taatattttg
     4261 aaccgaaaac taaacggaac aattcttcta ctactttgtc tccatcgtgg aagacaccac
     4321 catctttcaa ttccaaaaag tcggtggaaa tcactcgaca tccttcattt ctcaatccgt
     4381 tgaagtcgtg tgcgacttgt actagatact catcatagat ctctgggtcc atataatctt
     4441 caggcacttt ttccgtattg accaatactg tatcgataaa aggctgattc aaatgcttgt
     4501 gtaaaacacg tatatgatcg gcatctgtga aatgttccgt ttcccctttt tgggtcatga
     4561 tattacagat atagacggtt tcagcagttg tcttgagcat tgcttcgccg atttctggta
     4621 tcactaaatt tggaaggata ctggtgaata aacttccggg tcccaaaacg accatgtcgg
     4681 cgtccataat tgctgaaacg acttttcgag ccgctttggc ttcactttta tcatttgtgt
     4741 tgcagacata cacacggtca atggttttac ggtcgatcgc gattttagat tctcctactg
     4801 cagtcgttcc gtctttaaag acagcatgca aggtcaaagg agtttctgaa gaaggataaa
     4861 catgtccatc tacatgcatc atttttgcta ataattgaat cgcctcataa gtgcttcccc
     4921 gcatctcgga aactgcggca atgatcagat taccgatcgt atgattggct aaaaactgat
     4981 cttccttttt gaaacggtat tggaaaatat cttcatagaa ttgtggcata tccgataacg
     5041 ccactaagac atttctcaag tcaccaggag gagccatagc gatcgattct ctgattgcac
     5101 cgctgcttcc cccatcatct gcaacagtca ctactgctgt gatatctgct ccttgatttc
     5161 gcaaactttt caggataact ggcagaccag tgccaccgcc gaccactacg atctttggtt
     5221 ttcttatccg gtaggttttc attttcacga ccggttcacc gtctctttcc ttttatcctt
     5281 gtcccgatgt gtcacattga ctttatagtt tttccctaaa gcatttgcca aacgttcagt
     5341 caatgccaca gaacggtgtt gaccgcctgt acagccgatt gcaactgtca agctgctttt
     5401 tccttcttta atgtatccag gcaatacagt ttccagaaga tccaaaaatt tatggtagaa
     5461 tgattgcgtt tcatcaaagg acatcacata atcatagact ggctgatctt ttccggtcaa
     5521 tggtcttagt tccgggatat aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc
     5581 cgaatcgatc ggcaacccgt atttaaaacc aaatgataca aattcgatcc gaaatccttg
     5641 gtctcccttt gatttgaatg actcattgat tttttcacgt aattgtcgtg gtgtcaatgt
     5701 cgtcgtatca atgatcacgg aagcttgggc tttcaagtcg tctagtatcg cccgttcttt
     5761 acgtatccct tcagtgatca aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt
     5821 gtagcgagcc actagttctt cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa
     5881 gttcgtattt tcgatttcga tcagcatgtt ttggatttct tcaaagaagg aacgagaacg
     5941 taaatccact cctaaagcca tttttgtgac tttgcctgat tctttgatta attcccagaa
     6001 ttttggaatc aatcgaggcg gcatattatc gatacaaaaa tagcccatat cttcaaaact
     6061 ttggatagct actgttttcc ctgcaccgct catacctgtt atgatgacta attgcaaatt
     6121 atctgtcata attacgaact cctctcacat tgtgcatagt aaaattatat cattccgggc
     6181 gtatcattac cagttttctt tttcttttac aaaaagattt ggaccaaata tcttttagga
     6241 tatttgcccc aaatccaatt tttttacttt tccgatgctg tatccaatgc cttgcttctt
     6301 tcagctagcg ctcgttcaat cgtccgatct gctaaaatag attgggcatc gatcactgga
     6361 taatggttat cttcagcaaa ttcatgcatc aatgacaatt ctgtgcaacc taagataact
     6421 ttttcacagt ttaggcgctc taccgcttct tctaagattt cgtaatatag ctcttgattc
     6481 aaataatccg attccttgat ctcatgatag atcagataat tgattttttc ttgaagaaca
     6541 gtatcaggaa tcactgtttc gaatccaaga tttttgactt cgcgttcata gattccagct
     6601 ttcatacttc cttctgttcc tagaatcgct actctccctg tcgtatgctg acggacaagt
     6661 tcatttgctg cttctcttgg catatgaagg ataggaatat ccgttgccgc ttgtaattct
     6721 tcaaaaaagt agtgtgcggt gttacaagtc aacacaatga agttcggctg caataaattt
     6781 tgtttctcaa tatcatccag cagaaacggc attggatttt cttctgatcg atctaaaata
     6841 taagctgtac gatcaggaac tgttgcatga ttgaataaga cataattcaa atattcttga
     6901 tcttttgtag ctttcgttcg gtggttgatc aagcgaacaa aactttctgt cgccatcgtg
     6961 cccattccgc ctaaaatact gaaaaaattc tccatgaact agccttcttt cacatgaaaa
     7021 tactttttga atcttggaat atagttatgg aacatatatt tcatcaacaa ccagcgcttg
     7081 attgaccggt ctttttcata aaacacagta gtaccgtaac gattttcttt gatcatttgc
     7141 tcagctagtt ttttgtcttc attttcccga gcatattcta aaaagatttt tttgggcaca
     7201 cccatccata atcttgcttt atctgattga ttcgttccat aggtcgtttc aacaaagggt
     7261 ttgttaaaca ctcgatcttc tgtgacgaaa cgagccagat tcaagccgtt caatgtcaca
     7321 aagaaactgc tacgaccttg tctcaaattg atttcaaata atttatattc tccatctcta
     7381 ggatcatact tcatatcgaa attggcaaag cctgtatatt cgattttttc caaaaatgcc
     7441 ttaatggttt gatagatttt ttcgttataa tctggcatga tcacaacgta attaccaatc
     7501 gatgctggtg taggatcttc taacagtgga tgaccaagac acatcatccg aacttgatga
     7561 tcttcatcta catacgcatt tagcacacgc atattactat catcacccgg gataaaatct
     7621 tgtacaatca tttcactttt ataaccggct tcgtatatcc ggcctaaaat taaatcgaac
     7681 tcttctctag tctctaaaat aaaagctttt tttcttcctt caaattggac agaaagatac
     7741 tccacactgt ttgcaggttt caaagcaact gggaattcaa aaggcaattc ctgttccaaa
     7801 tgtccattta ctaacatttc ttctctgacg atcaatgttt taggatacgg aagatcatat
     7861 tcttcacaaa cttcataaaa gcttacttta ttgatcagtc tttcaaataa agaataatca
     7921 atatatggac agataaaata ttctgacagc tcttgtttat gttgggagat caattctgca
     7981 tatccatcac cacaagcaat caaaagatat tttttactct tatcagaata tctttctttt
     8041 gccaaacgaa gcatcgtctc gataaacaca gggtctttat caaatcctgg aataacttct
     8101 acattgacaa ttttactgta gcgggtaggg gctaactgat ccgaagcata tgcttggcag
     8161 attttgccgt atgcttcatt aaaagaacgt gccataccat aaacgttcat gtcgcttcct
     8221 aataatatag ggataaattc ttcattttca atactgttca tcattatctc tcacttactc
     8281 ataatctctt gtttttcttt tccaactaca caaggaatat cataccataa agaaaaaagc
     8341 aatcaaagga aactcctccg attgctcaat tcttttttga acggattgaa cttataggaa
     8401 gtttttgtct ataccaatca gtgtagaaag aactaccatg taatgattct agaacactga
     8461 tattaataaa acaagcaaaa attcattttt ttatgttttt ttaactatct cagctatcta
     8521 tcctctttat accattattt ttttcaaata gtgacctgta taactttctt ttacgttaat
     8581 gatttcttct ggtgttccag tcgcaaggat cgttcctcca ccttctccac cttctggtcc
     8641 taaatcaatg atataatctg ctgttttgat tacatctaga ttgtgttcaa tcactaaaac
     8701 tgtgttacca gcatctacta atctttgtaa tacatgcaac aagcgggcga tgtcatcgct
     8761 atgaagtcct gtcgttggtt catctagtat atagaaattc tttccattag agattttgtg
     8821 caattcactg gcaagtttca tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg
     8881 ccccatcgtc acataaccta agccaacatc aacaatcgtt tgcagtttgc gatgaatctt
     8941 tggaatgtgc ttgaagaatt ctacagcatc ttctactgtc atttccaaaa tatcagcaat
     9001 gctttttcct ttgtaatgca cttctaatgt ttcagagtta tatcgtttgc catgacatac
     9061 ttcacaagga acatagacat caggcaaaaa gtgcatttcg atcttaataa ttccatcccc
     9121 gcgacaagct tcacaacgac cgccttttac gttgaaacta aaacgccctt tcttataacc
     9181 ccgcattttt gcctcgttcg tttgagcaaa taacccacgg atatcatcaa atacacttgt
     9241 ataagtcgct ggattacttc tcggcgtccg gccgattggg ctttgatcga tatcgatgat
     9301 cttttcgata ctttcgtagc cggaaattgt cttgaattta cctggcttag cagaattctt
     9361 atttaatttt tgcgctaacg atttctttaa gatactattg accaatgtac tctttccaga
     9421 accagacaca cctgtgactg ctacaaattc tcctaatgga aattcaacac taatattttt
     9481 cagattgttt tctgaagcac cagtgatctt gattgctttc ccattacctt tacggcgttc
     9541 ttttggtaca gggattgatt ttttccctga aagatatttc cctgtcaaag aattttcatc
     9601 attagctact tcttcaggag tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc
     9661 aggtccgacg tcaatcagat aatctgctgc ccacatcgta tcttcatcat gttcgactac
     9721 aacgagggtg ttgccaagat cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc
     9781 gcgttgatgc aatccgatcg atggttcatc taaaatatac aaaactccag aaagattcga
     9841 tccaatttgt gtagccaatc tgattcgctg cgcttctccc ccagataatg ttccggaagc
     9901 gcgactcaaa gttagatagt ctagtcccac attttccaag aatgaaagtc tgtcatttac
     9961 ttcttttaaa atcggtttgg caattacttt ctcttgttca gataacgtta agtttgagaa
    10021 gaaatctact gctttttgaa tagacaactc atttgtttgc ccgatattcg ttccattgat
    10081 ttgaacagat agagcttggg gattcaatcg ataaccttta catgtctgac aagttaattc
    10141 tgtcatataa agacgcattt gctcacgcgt gaaatcactg ttcgtatcat gataacgacg
    10201 ctcgatattc ttcaataccc cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc
    10261 attttcataa tggaaatgga aaagatcatc tgtcccattc aagatgatat cctgatgctc
    10321 ttccggtaat tcttcgaatg gtgtatctaa atcgataccg aaactttcac aagcctgcgc
    10381 taacatttga ggataatact gagagctgat tggattccat ggaacgatag caccttgact
    10441 aagtgtcttc gttctatctg gaatcactaa atcttgatcg acttctaatt tgacacctaa
    10501 accatcacag tctggacaag ctccaaaagg cgcattgaat gagaacagtc ttggttctag
    10561 ttcgccaaca gtaaatccgc aataaggaca ggcatagtgt tcgctaaaca acatttcttc
    10621 ttcaccgatc acgtcaacca gagcatagcc gtctgccaat cgcagagccg cctctaaaga
    10681 atcaaataga cgagaacgaa tcccttcttt cacaatgatc cggtcgatga cgatcgcgat
    10741 atcatgtttt ttgtttttct ccagctcagg cacttcactt aaatcataga tctcaccatc
    10801 gactcgaacg cggacgtatc cctctttttg gattcgttcg aatatttttt tatgttgtcc
    10861 ttttttcttt accacgatcg gtgccaacac ttggatcttt gtgcgctcag gcaaagccat
    10921 tacttgatct gccatttgat ctactgattg actggtaatc tcgatatgat cgttgggaca
    10981 aatcggatgt cctacacgtg caaacaacaa gcgtaaatag tcattgattt ccgtcacagt
    11041 acctactgtc gaccgaggat ttttacttgt tgttttctga tcaatagaaa tcgccggact
    11101 caatccgtcg atgctatcta cgtccggctt atccatttgt cctaaaaact gacgagcata
    11161 agcagaaagg ctctctacgt atctccgctg cccttcagcg tacagcgtat caaatgctag
    11221 ggaacttttt ccagaaccgg acaaacctgt aacgaccacg aatttatctc gtggaatcgt
    11281 tacatctata tttttcaagt tatgggcacg tgcaccgtga atcgtaattt tatcgttagc
    11341 catgttgtcc tcctactttg aagcttttaa ttctaaaatc atatctcgca cattagcggc
    11401 tgtttcaaaa tccaatgctt tcgccgcatt tttcatttct ttttctaatt tcatcaataa
    11461 cgtatctttt tcttctttcg tcatttcttc atacgagact tgtaccgctt cttttgtatc
    11521 atcttccgat tctttcgtaa tagaaatcaa atctcggatt tctttaatga tcgtttttgg
    11581 aacgatccca tgttcttcat tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc
    11641 catcgctaga cgcatagaat ctgttacttt gtctgcgtac atgattactt taccttcttc
    11701 attccgagca gctcgtccaa tcgtttggac taaggaacgt tcactacgta aaaagccttc
    11761 tttatccgca tccaagatag caacaaggga aacttcaggt acgtctaatc cttcacgaag
    11821 caaattgatt ccaactaaca catcaaattc acctaatcga agatctcgaa tgatttctgt
    11881 tcgttccagc gttttgatat cactatgcaa atatttgact ttgatcccta attctttgaa
    11941 ataatctgtg aggtcttctg ccattttttt cgtcaatgtc gttacaaaca cccgctggtc
    12001 tttttcgact cgttcattta tttcgcctac tagatcatcg atttgtccca tgattggacg
    12061 aacttcgatc actgggtcaa gcagccctgt cggacggatg atttgttgga taacggtatc
    12121 tgtttgttca tgttcatatg gtcctggcgt cgcagagaca taaatgatct gattgacatg
    12181 tttttcaaac tcttctaacc gaagtggccg attgtctaaa gcagatggca aacggaaacc
    12241 gtaatctact agcatctgtt ttctcgctcg gtcaccatta tacattcctc tgatttgagg
    12301 catggttacg tgcgattcgt ccgccacgat caaaaaatct tcaggaaaga aatccaatag
    12361 cgtataaggc ggttcacctt ctttacgtcc gtccatatgc cgagaataat tttcaattcc
    12421 cgacgtgtag cccatttcta acatcatttc aatatcgtaa ttggttcgct gttctaaacg
    12481 ttgagcttcc agaagtttgt tttcatttcg caagacagtt aaacgctgtt ctaattccgc
    12541 tttgatattc gctacggcat gctccatatg ttcatcattc gtcacaaagt gagtcgccgg
    12601 gaagattgct acgtgttccg tttctcctaa aacttcacct gtcagtgcat tgacttctcg
    12661 gatacgttcg acctcatctc caaaaaactc cacacgtaag gcacgttcat ctcttgaagc
    12721 cggaaaaatc tccacgacat caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc
    12781 atttctttcg aattggatac tgactaagtc acgaatcaac tgattgcgat ctagttctgc
    12841 tccctgtcga atcgaaacta cttgtttttg gtactcaaaa ggcgagccta atccaaaaat
    12901 acatgaaacg gatgcaatga cgatcacatc atttctttct aataatgaac tagtagccga
    12961 atgtcgtaat ttgtcgatct catcatttac acttgaatct ttttcgatat acgtatcgct
    13021 tgaaggcaca taagcttcag gctgataata atcataataa gaaacaaaat attcaacagc
    13081 attgttaggg aaaaattcct tgaattcccc gtacagctga ccagccaatg ttttgttatg
    13141 ggcaataatc agtgttggtt tattcacttt ctcaataaga ttggaaatcg tatatgtttt
    13201 ccctgttcca gtcgcaccta ataagatttg tgcttttttc ccgccgacta cgccatccac
    13261 taattggtta atcgcttcag gctgatcacc agccggttgg tatttcgaga caagctcaaa
    13321 gtgtcgggag gtatctctct caatcattct tcgtcctcct tttcattcat tccttttcat
    13381 tttacgacca ataaaaagaa aaggaaagca aaggcattcc ttactaaaaa attataccac
    13441 accgaacata ctttcgccac ttttttatct tattatacat gttatgtttt ataacataaa
    13501 atgttagaaa ctcgcatttt tttcattttt tcgagcttac agtcttgtct gattcagcac
    13561 ttttcgccta taataataaa atattggaaa tttaggaggt tgattatgaa aagaaagcat
    13621 ttatttcact catttgtttt cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt
    13681 gcacaagctg acaacactac aacggcagag tcaagcacaa gcgagacaat gcaaaaaata
    13741 gagcccaaaa aagacgtata cgtaattgcc agtgactctg cttttgctcc atttgaattt
    13801 caaaacgcaa agggcgagta cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa
    13861 ctgcaaggat ttaacatccg aatggactac cgtggtttct ctgctgcttt gcaagctttg
    13921 gaatcaggac aagcagacgg aatgattgcc ggaatgactg taacagatga acgaaagaaa
    13981 agctatgatt tttctgattc ttactttgat agcgggatcc aattagctgt caaaaaagga
    14041 gatacttcga tcaagtctta tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt
    14101 accgaaagtg ctgaattttt aagcgaccat gaatctgaat atggctattc tatcaaatat
    14161 tttgatgctg ctgatcaatt gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg
    14221 gatgattatc cggtaatcgg ttatgcgatc aaacaaggac aagaattgga aacaccgatc
    14281 aaaaaagaat ctggcggaaa atatgcattt gccgttaaaa aaggacaaaa tcctgagctt
    14341 cttaatatgt tcaatgaagg attgaaagaa ttaaaaagaa caggcgaata cgataagatt
    14401 attaaagaat atgtagcgga tggttcagaa acttccagtg agtcttccac tgctgatgaa
    14461 tcaacatttc ttggacttat ccaaaacaac tatcaagcat tgttgaaagg actttggaga
    14521 acaattctcc taactttgat ctcctttgct ttggctatag taatcggtgt gatttttgga
    14581 ttattcagtg tagcaccaat acgcgctctt cgcacaattg ctagtattta tgtcgatatc
    14641 atccgaggaa ttccaatgat ggttcttgcg ttctttattt tctttgggtt gcctggtatc
    14701 gttggattta cgattcctga cttccttgct ggtgttatca ccttgactct taacgcaagt
    14761 gcttatatcg cagagattgt tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa
    14821 gcttccagaa gtttaggtct ttcttataat cggacaatgc aaaaaatcat tttgccgcaa
    14881 gcgatacgca tcatgatccc atcattcgtc aatcagttcg ttatctcatt aaaagacaca
    14941 acgatcattt ccgcaatcgg tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca
    15001 cgtacaacac aaagtacgta tgtttacttg atcattgcga tcatgtactt gattttgatc
    15061 acagcattga caaaattagc aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa
    15121 aatcttagtt gagcatttag tcaaaaaata cggcgataac acagtattaa acgacatcaa
    15181 tgtatccatc aacgaaggag atgtcgtctg tatcattggc ccttcaggtt caggaaaaag
    15241 tacattcttg cgttgcttga atcgtttaga agaaccttct gacggtgaaa ttattattga
    15301 cggcgcccac ttgatggata aaagcacaga tatcaacttg gtgagacaac atatcggaat
    15361 ggtattccaa cactttaacc tattccctca tctttctatt ttggaaaaca tcattttagc
    15421 accgatggac ctgaagaaag aatctagaga agaagcagag aaaaaagcaa tcaaattact
    15481 gcagacagtt ggtctagaag ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca
    15541 aaaacagcgt gtggctattg ctcgtgctct agcgatgaac cctgacatca tgctttttga
    15601 tgagcctact tctgcactcg atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa
    15661 gttagcagaa caaggtatga cgatggttat cgttacgcac gaaatggggt tcgctaaaga
    15721 agtggccaat cgcgtcatgt tcattgacga cggaaacttt ttagaagatg gaacgcctca
    15781 acaaatcttt gaaaatccgc aaaatgaacg gacaaaagat t
//
LOCUS       DACCWV010000064        15534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCWV010000064 DACCWV010000000
VERSION     DACCWV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.064"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            19..294
                     /locus_tag="IVW84_001660"
     CDS             19..294
                     /locus_tag="IVW84_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917855.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            336..758
                     /locus_tag="IVW84_001661"
     CDS             336..758
                     /locus_tag="IVW84_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917856.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            777..2369
                     /locus_tag="IVW84_001662"
     CDS             777..2369
                     /locus_tag="IVW84_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917857.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2381..2716
                     /locus_tag="IVW84_001663"
     CDS             2381..2716
                     /locus_tag="IVW84_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917858.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2863..3057
                     /locus_tag="IVW84_001664"
     CDS             2863..3057
                     /locus_tag="IVW84_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917859.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            3050..3622
                     /locus_tag="IVW84_001665"
     CDS             3050..3622
                     /locus_tag="IVW84_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917860.1"
                     /translation="MFRQTIFYKEETDMAEEKEPLDNSRLGKSKRKLVRLQNELNNQF
                     QKLFDHQKLTNGQPMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEEI
                     KEMGYNKYGGLDMTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSE
                     KGKENLLPEVQAIIDSGRVTQWKKIRPFIF"
     gene            3649..5460
                     /locus_tag="IVW84_001666"
     CDS             3649..5460
                     /locus_tag="IVW84_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7917861.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            5521..6981
                     /locus_tag="IVW84_001667"
     CDS             5521..6981
                     /locus_tag="IVW84_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7917862.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            6992..9196
                     /gene="topB"
                     /locus_tag="IVW84_001668"
     CDS             6992..9196
                     /gene="topB"
                     /locus_tag="IVW84_001668"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7917863.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            9435..10028
                     /locus_tag="IVW84_001669"
     CDS             9435..10028
                     /locus_tag="IVW84_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7917864.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            10044..10943
                     /locus_tag="IVW84_001670"
     CDS             10044..10943
                     /locus_tag="IVW84_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7917865.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            10946..11431
                     /gene="ssb"
                     /locus_tag="IVW84_001671"
     CDS             10946..11431
                     /gene="ssb"
                     /locus_tag="IVW84_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7917866.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            11797..12057
                     /locus_tag="IVW84_001672"
     CDS             11797..12057
                     /locus_tag="IVW84_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917867.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            12498..12704
                     /locus_tag="IVW84_001673"
     CDS             12498..12704
                     /locus_tag="IVW84_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7917868.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            12704..12955
                     /locus_tag="IVW84_001674"
     CDS             12704..12955
                     /locus_tag="IVW84_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917869.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            12971..13408
                     /locus_tag="IVW84_001675"
     CDS             12971..13408
                     /locus_tag="IVW84_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917870.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            13853..14032
                     /locus_tag="IVW84_001676"
     CDS             13853..14032
                     /locus_tag="IVW84_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917871.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            14150..14413
                     /locus_tag="IVW84_001677"
     CDS             14150..14413
                     /locus_tag="IVW84_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7917872.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            14407..14757
                     /locus_tag="IVW84_001678"
     CDS             14407..14757
                     /locus_tag="IVW84_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7917873.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(14781..15395)
                     /locus_tag="IVW84_001679"
     CDS             complement(14781..15395)
                     /locus_tag="IVW84_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7917874.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKRSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         5853 a         2522 c         3160 g         3999 t
ORIGIN      
        1 ataaatttgg aggaaaatat ggacttatta attagaaata ttgaagaaaa gtatattaat
       61 aaaattgaca aacgttgtga agaacttacg gtcaagactg gcaaaaaatg gagtcgaaat
      121 caatacctaa aagttttgat cgaaaatgat tttgatcacg ctcttctgaa ttataaaaaa
      181 gaccaatttg atcggttgtt agaaaaattt gtggatattc aaacttataa tacgaaaact
      241 ttagaagaat atattgcaac aaataatcaa ttaattggtt tgttaattga ataataaaaa
      301 tagtattgtg aatttataaa aggaggaaat caattttggc aaaagcaaca agaaaacatg
      361 tatatcttta cgatatgcat atcgaagtac ttgatgaaat aatttctact catgaagata
      421 tatctaatta tagtgaagca gtcagacatt tgtgtatgat gtatgatcca aaaatcgaag
      481 aaaaaataaa tccaattcaa attaaattga atgcaatggg aaaagaaatt tctattttaa
      541 ccgaaatggt ttcagagttt gcggatcttc atttgaaaga tacaggaata ctaattggta
      601 caaattcaac tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt aaatccaatc
      661 aaaccaaaaa atattcttct aagttaaaga aaccagtgaa tgaatctgtt cataccagag
      721 tagatccatt tgatccaacg aagattctaa aaagataaaa gtagaaggag aacgtgatga
      781 ataaatcgcc tgcagttatt cttacttgtc aatttacctt gcccaataaa aaatcttttt
      841 caacatacat tgattatatg acaagagaaa aagcactgga agaaatggaa cgacgtacgc
      901 ccgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg
      961 aaggagaaac tttttcggaa agtaagacgg ataaagaatt cagtgaaacg tcgaatgaag
     1021 ctcaatcgtt gatggaacat ggaatcaatt ttgacaagtt agaggaacaa gattttacca
     1081 aatatctttc ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa
     1141 gggaacaaaa agaataccaa cggttgaacc aagcaatgaa aaaatatcaa acagagccac
     1201 ataaaaaagc ttcaggaaat cttcctggag ttttttctat ggcttcagac gaagtgaaag
     1261 gaagtgatct gaaggagatc aaacaaatct tccaaagagg acaacaaaaa ggctcaatca
     1321 tttatcaaga tgttatctct cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc
     1381 ccaaaacaga taccttagat gaagaaggtt tgaaagcagc tggaagaaaa atgatggaga
     1441 cattattcga gaaagaacaa ttgaatgaaa caggttactg gatggcaacg attcaccgta
     1501 acactaagca tatccatatt cattttgcag ttgttgaaaa agaaaatacg cgtaaaccgt
     1561 atactgagat tgaaaatggt gtgagttata tcgtaccgaa ggggaagaga agacaagcaa
     1621 cattagacgc aatgaaaacc aattatgtac atgagttaga acgtttctct tatgaaaagg
     1681 atcgaatgat cggtatggag aaacgaaatt tattgacacg aaaaagtgat ttgcgtaaca
     1741 ccttaacaaa agtagtaaaa gaaccagcac gttacgatcg acacgccatg aacttattaa
     1801 atgaagtata taaaacccta cctgaaaaac gttcggaatg gaattatgga gatgaaaatc
     1861 gaaccaagct ttcaccattt acaaggggaa aacttgatga gttaaccaaa tatgttttaa
     1921 caaaagaacc tggatatcaa gaatatatac agatttcaaa agcattgaaa gaagaagcca
     1981 aaagccttta tggggaaagc aaaagagaaa caaagaatgc agaaaaaaac gccctctttg
     2041 atttaaaaaa gcgcacagga aatgcgattt taacagaact gaagagacag gatagtcaaa
     2101 taaaaccatt aatagaaagt gtcaatcaga tgcaatctat ttacaatgaa cgatcctttc
     2161 gaacactcaa acagttgcca acaaatgaat acaagtatag ttatcaagaa ttcgtaaagc
     2221 gtaggaaaca attccaacaa agagttgtca cgcagcgaag cttgcgtcaa ttaaatcgaa
     2281 caattaatgc acatgaagaa aaataccacg caagtaaagc atatgaagag caacagcgaa
     2341 gaatccaaat ggaacaagaa agacaatagg aggaagagaa atgaaacaat atttagatca
     2401 atggaaagtc atagaaggat cactaaaaga agaacgaatc gaacaacttc ctgattgttt
     2461 agaaaaagag catttgtttc agataagcga aatgttgaga aacgaacagt ttgatccaaa
     2521 tcaattttta gttgttgagt acccagctac tggtgtgtat tgttgtaatc atgtgaaggg
     2581 tgaaaaatat tttattattc aagaatacga aggtaagtta gcgccttatt atactacttg
     2641 ggaaataaat gaagagggaa tcaataattt cccatgtgaa agcattgaag agtctatttc
     2701 tctcacggaa tgttaaataa atagagacgt aaaagagtcc tgaacgtgat tgtccaggac
     2761 tctttttcaa tgaattgttt tccgccaatt cactgagtgg tgcgattcaa ttttattata
     2821 tcaaaagaaa gtgagattcc aaagtgaagg gaggaagaag aaatgactca gacaacgatt
     2881 ggtgttgtgg ttgaaaaaag cagggataat ctcgtttttg tgacagaaat tcaaacaggt
     2941 agagcttttg tagtcacaga caaagctgca aaagcgtatc aaaatggcaa tattttaacg
     3001 ttaaatctga ctacgaagat gttcgttgat gcagtagaaa attatccatt tgtttagaca
     3061 aacgatattt tacaaggagg aaacggacat ggcagaagaa aaagaaccac tagataatag
     3121 tcgtttggga aagtccaaaa gaaaattagt acggttgcaa aacgaattaa ataatcaatt
     3181 tcaaaagcta tttgatcacc aaaaattgac aaatgggcaa ccaatgaatg acaaacgaaa
     3241 tggtcgttca tggtttaacc agcaagaaaa aatagaaaat aaaatacaaa gtctgagaga
     3301 agagatcaag caacaagaaa aacaagtaga aaaacttgaa agacaagaag agataaagga
     3361 aatgggctat aacaagtacg gcggactgga tatgacgatc gagaatattc caagaatcaa
     3421 agaagaaatc gaacgctttg aaaaaggtga atcgactttt tcagccgcta cgatccgaaa
     3481 ataccaacgg aaattggaaa cattagaaca gctgaaagaa agatcggaaa aaggaaaaga
     3541 aaaccttctt cctgaagtac aagcaatcat tgacagcgga cgagtgacgc aatggaaaaa
     3601 aatccgacca tttattttct gaaaggctat cgaaaagtcg ctttagaact gaatgaaaac
     3661 ggggaatttg aggaatctaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
     3721 cgtgaattac ttgaagaggc tgtaccaact gattttttga aggagaaaga aaacgatcat
     3781 ttttatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
     3841 gagatcgtaa cattcgaatt gatcgatcaa ctcaaaacgt tggataacca atacgccgca
     3901 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtcgatcag
     3961 atccgaatgg atctagggga tggcgtcaac agtaatcaac ccatctattt agagttagca
     4021 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
     4081 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
     4141 acggcagaag agattaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
     4201 aaaaagcata ctcattctcc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
     4261 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
     4321 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
     4381 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
     4441 cgagaaatag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
     4501 atcaaaaaaa cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
     4561 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
     4621 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
     4681 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
     4741 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
     4801 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
     4861 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
     4921 tttggaatgg acacgtcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
     4981 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
     5041 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
     5101 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
     5161 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
     5221 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
     5281 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
     5341 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
     5401 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
     5461 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
     5521 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
     5581 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
     5641 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
     5701 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
     5761 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
     5821 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
     5881 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
     5941 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
     6001 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
     6061 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
     6121 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
     6181 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
     6241 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
     6301 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
     6361 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
     6421 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
     6481 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
     6541 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
     6601 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
     6661 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
     6721 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
     6781 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
     6841 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
     6901 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
     6961 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
     7021 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
     7081 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
     7141 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
     7201 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
     7261 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
     7321 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
     7381 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
     7441 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
     7501 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
     7561 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
     7621 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
     7681 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
     7741 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
     7801 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
     7861 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
     7921 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
     7981 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
     8041 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
     8101 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
     8161 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
     8221 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
     8281 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
     8341 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
     8401 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
     8461 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
     8521 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
     8581 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
     8641 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
     8701 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
     8761 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
     8821 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
     8881 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
     8941 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
     9001 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
     9061 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
     9121 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
     9181 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
     9241 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
     9301 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
     9361 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
     9421 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
     9481 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
     9541 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
     9601 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
     9661 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
     9721 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
     9781 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
     9841 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
     9901 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
     9961 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    10021 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    10081 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    10141 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    10201 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    10261 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    10321 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    10381 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    10441 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    10501 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    10561 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    10621 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    10681 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    10741 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    10801 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    10861 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    10921 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    10981 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    11041 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    11101 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    11161 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacagc gtgtctatgt
    11221 gactgaagtt gtggccgata atttccaact attagaaagt aaagcagcga cagaaagccg
    11281 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    11341 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    11401 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    11461 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    11521 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    11581 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    11641 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    11701 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    11761 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    11821 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    11881 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    11941 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    12001 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    12061 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    12121 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    12181 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    12241 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    12301 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    12361 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    12421 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    12481 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    12541 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    12601 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    12661 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    12721 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    12781 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    12841 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    12901 agaaaacatg gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg
    12961 agaaaaaaat atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat
    13021 ggcacaaaaa atggaggata tgacgttcct atacaacgaa acacgtgttc caaagaaaca
    13081 ttataaagaa aaattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa
    13141 tctcattgcg acgtactatc gaacactgga agaactgaaa aagcaaaatg caaaatggtt
    13201 tttccaagcc ttactatgct tggaagtagg cgtcaaacca agcacaatca aaccttctga
    13261 ataccaagct ttagaactaa cgtatgcgaa atttatcgaa acgaaaaaag ccaagactgt
    13321 ttcaagtgaa tggttagatt attttgaaaa tatcaataag tatggcgcat attacaccat
    13381 gaaaaaggaa gacaacgaaa atgaataagg tgatttatca actaacaacg gattccatat
    13441 caacgatttg aaagaaaaaa aggtcaataa tcatttatac cgaaaagtga gaacgtgtga
    13501 aataaggaca aaaaaagcct agtaactaag ggaatggttc ttatttcgca ccaatcgctc
    13561 caaccgcccc cgccgcttag gtgatgcgaa ataaacgggc aaagcccgat aactcaagag
    13621 aaagaaggtg atccatttga acaagattat tcccttattg ttagtactgt ttgttggcga
    13681 aataattatg cgacatagtg cagctgtgca tttaaacttc gtagtatttt tggtaatact
    13741 ggtgttttgt ttgatctttt ggcgctttgt gttagccctt gtgagtctaa tattcaagtt
    13801 gattgcagcc atatttttca tagggttatt tcgatgaaag gagagagtac taatgccaat
    13861 tgcaatagga aataaacggt tacccgttac gttggatgaa aagagacaaa aagaattgca
    13921 gaaactaaag cagaagtacg gaaaaagtga atctaagatt atgtgtattg cgttagatct
    13981 attgatcgcg caagaaaaag caggatttga tgtaccagca ctcaaaaagt gacgtcactt
    14041 ttcatttgaa gaaacaaaag tgatagcact taatagaaaa ggataattaa gtatatacgt
    14101 tttttcttat gtatgttata ctatgtatat acagaaagag gtgtctacta tggaaatgtt
    14161 acctataaaa aaatggggaa atagtaatgg ccttagattg ccaaagtata ttatggaata
    14221 tcttggaatt catacagaag acaaagtgaa gataatacaa gaagaagtaa acggtcagaa
    14281 gcggctaatt atcgaagcgg ctaatataga aaatgaatta acaattgaac agttatttga
    14341 aaattacact gatgaaagaa atcatgtgac gattcaaaat ttaggtgaag cagtaggtaa
    14401 tgaaaaatgg tgagaaaacc tagacaaggc gatattctat tattgaatac agcacctcga
    14461 tctggacacg aacagacagg taaaagacct tatattgttt taagtcatga tattattgcg
    14521 gattatagta acgttgttac agttgctcca atttctagta caactagaaa ctatccgcta
    14581 tatgtaaata taaactcaga atatgaaatg aagacaacag gaaaagtatt attagatcaa
    14641 ctaacgacaa ttgattatga agccagagag tgtatttttt tggaaaaagc gcatgatagt
    14701 ttagttgagg agttattgat gaaagttaga acagtttttc aaaaagtaag caaataaaat
    14761 agaaacagca gcatgatcaa ctattcgtga ttgaccatgc tgtctttttt tattctatat
    14821 acggtttgtc tcgtaacatt gtagtctttt gcaatcttag cgatcgcaac accttttttt
    14881 agatcctcaa cgatgttttt ataaacaaga cgacgttggg gatcttttgc attggcactg
    14941 tatagcttag gacgtccttt ataaacaccg ttcgcttttg caatcttaat gccttgtgcc
    15001 tgacgtcgtt tagattcggt acgttcttgt tcagcgatca tggccaaaat ttgaatgatc
    15061 aaatctttaa tgaatttatc caggagagga ttgccaattg cttcggccat gatcggtaga
    15121 cttgtgatga tcagctgcac atttttcttc ttcaagtaat tgactgaagc gatgatctcg
    15181 tcgtaatttc ttccaagtcg atcaatagct tccacaataa aaatatcttg atctcggacg
    15241 aaatccagtg cttcttgaaa aacaggtcga tgagtaatcg ttgcgccaga tcgtttctca
    15301 atgaaaattc tctctgcacc gaattttttc atttcttcaa tctgccgttg ttcattttga
    15361 tcgatggatg agacacgtat gtatgcaact ctcaaaattt ttacctactt tctgcaacta
    15421 tttaaaaaac accctaatca tacacgagaa aaccagattt ttcaatgtcg cggtcgtgtc
    15481 tagttagggt ataccttata tagacaaaaa ctaatgatgg aggaaatagt atgt
//
LOCUS       DACCWV010000065        15292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCWV010000065 DACCWV010000000
VERSION     DACCWV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.065"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            19..1137
                     /locus_tag="IVW84_001680"
     CDS             19..1137
                     /locus_tag="IVW84_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7917875.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1265..2641
                     /locus_tag="IVW84_001681"
     CDS             1265..2641
                     /locus_tag="IVW84_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7917876.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3042..4622
                     /locus_tag="IVW84_001682"
     CDS             3042..4622
                     /locus_tag="IVW84_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7917877.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4820..5044
                     /locus_tag="IVW84_001683"
     CDS             4820..5044
                     /locus_tag="IVW84_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7917878.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5249..5569)
                     /locus_tag="IVW84_001684"
     CDS             complement(5249..5569)
                     /locus_tag="IVW84_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7917879.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5717..7951)
                     /locus_tag="IVW84_001685"
     CDS             complement(5717..7951)
                     /locus_tag="IVW84_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7917880.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8430..8696
                     /locus_tag="IVW84_001686"
     CDS             8430..8696
                     /locus_tag="IVW84_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7917881.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8696..10423
                     /gene="ptsP"
                     /locus_tag="IVW84_001687"
     CDS             8696..10423
                     /gene="ptsP"
                     /locus_tag="IVW84_001687"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7917882.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            10554..11633
                     /locus_tag="IVW84_001688"
     CDS             10554..11633
                     /locus_tag="IVW84_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7917883.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            11650..12873
                     /locus_tag="IVW84_001689"
     CDS             11650..12873
                     /locus_tag="IVW84_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7917884.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            12962..13849
                     /locus_tag="IVW84_001690"
     CDS             12962..13849
                     /locus_tag="IVW84_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7917885.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            13935..14165
                     /locus_tag="IVW84_001691"
     CDS             13935..14165
                     /locus_tag="IVW84_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7917886.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14222..15187)
                     /locus_tag="IVW84_001692"
     CDS             complement(14222..15187)
                     /locus_tag="IVW84_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7917887.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         4962 a         2629 c         3121 g         4580 t
ORIGIN      
        1 aagaaaggag gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg
       61 cttgaattat tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc
      121 gaaccgatcg ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt
      181 tactatttat tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg
      241 gctgtcgcaa ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc
      301 atcccaagtc tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa
      361 caggtagaaa gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc
      421 aataaatatt ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt
      481 ggagtatcca atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc
      541 attacagcac cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac
      601 atccaacagt ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg
      661 aataaaacat tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc
      721 ttcacatttt taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt
      781 gcgggattga caaacctaat tccatattta ggaccttatc taggactagc acctgcattt
      841 ctagtaacag tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc
      901 gtccaacagt tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc
      961 catccattaa cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt
     1021 atcttcttag gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa
     1081 atcgtcaaag aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta
     1141 gttttagtac acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg
     1201 ctatagtgcc acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc
     1261 gattatgaat gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt
     1321 tttgacattg atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa
     1381 gaatcgaatc gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat
     1441 attgcagaat ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat
     1501 tttgtctggt gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg
     1561 cggcacagcg gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc
     1621 gattgttttt ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc
     1681 aaagttcact tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct
     1741 gctttctgct tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga
     1801 ttcaaaaatg accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa
     1861 tgaagaatta gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt
     1921 catggtccca cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt
     1981 gaatgaagtt ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa
     2041 agtagtcgga atccttcata caaagaattt actaaaagct gcccataaat tcggctttga
     2101 taatttagat atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat
     2161 tgatgatctg ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga
     2221 atatggcggc gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga
     2281 aatcgatgat gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata
     2341 tatcatccaa ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca
     2401 tatgagtgat gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga
     2461 tgaaggcgaa aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga
     2521 aggttctcga atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga
     2581 acctgaagaa gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata
     2641 attggaaact tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt
     2701 ttagtcagct gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga
     2761 atccatgcta tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc
     2821 attaagtaat aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct
     2881 tattgctgat aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc
     2941 ttttgtcaca gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag
     3001 agaaaatata aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa
     3061 agcaacaagt ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa
     3121 caacgattac agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa
     3181 aaggcaaaaa aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg
     3241 ggatttctgt tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct
     3301 tggatacacc aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg
     3361 atagtgcggt catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat
     3421 tccaagttgt aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg
     3481 atggtcgtga accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt
     3541 atccgatgaa ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc
     3601 ataatcgtgt agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg
     3661 atgaagatgg actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag
     3721 tcttagaaga agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg
     3781 ctcgtggcga tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga
     3841 cttttttaga gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg
     3901 atcagatggt aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa
     3961 atatgaatcc agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg
     4021 aacgaggaat ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca
     4081 cacaatttat ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg
     4141 gtgtatatga tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag
     4201 ttgcttatga agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa
     4261 atgtcatgaa acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga
     4321 ttcagcttta taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac
     4381 aattcgaagt attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc
     4441 caatgggaca caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt
     4501 caagtcgcaa tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc
     4561 aatttgcaga acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt
     4621 aaagaagtct tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag
     4681 cctctcatat tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt
     4741 aaaatcatag aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa
     4801 agaaaggaga gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa
     4861 aaaagaagag atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga
     4921 gcaaattgca caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga
     4981 cacgcgtaga actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag
     5041 ctagaaagaa aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca
     5101 caaaaatttg aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc
     5161 tttcgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
     5221 ctctttaatt attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt
     5281 ttggaatcga gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt
     5341 aaacggcgtc tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct
     5401 ggattttacg tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga
     5461 caatagtggt atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct
     5521 ggtttaatac aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct
     5581 cttttatgta tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac
     5641 agtattttga cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca
     5701 cgctataaaa ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg
     5761 gtttagaagt aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag
     5821 aagggtgatc aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc
     5881 gaagaggacg agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat
     5941 tcgtaggaac ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca
     6001 ttagtgtgac gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt
     6061 caaaacggtt cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag
     6121 taacaccttc gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat
     6181 tacttgtcat aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca
     6241 atcgaccatc atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta
     6301 cttcatctaa caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag
     6361 cttcttcgta tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt
     6421 ccatatattc agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg
     6481 caagttgttt cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc
     6541 cgattggacg attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc
     6601 gatctacagc gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca
     6661 gttgtgtctg ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga
     6721 ttttttccat atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt
     6781 tcattttttg aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt
     6841 ctcgagaagc taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag
     6901 gatcaacgat ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg
     6961 ctttatccgg caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga
     7021 tggcctcatc agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca
     7081 aaatactgat cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt
     7141 ctaaagcagc gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt
     7201 gcaattctcc tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac
     7261 cagcagaacc agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg
     7321 cttgacggat ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag
     7381 taccttggac taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg
     7441 gtacatcacc atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac
     7501 ctggttctcc aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc
     7561 gtttgatttc gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg
     7621 tgatattgat tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac
     7681 cattgaagtt gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct
     7741 gttgcatttg gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat
     7801 cattttgggc catattagtc agacctccat tttgttgatt tttgattttt tgataacagc
     7861 tctgacagta atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg
     7921 cctcattctt gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg
     7981 gtacatccat attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta
     8041 agagatttat gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt
     8101 attatactaa aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc
     8161 aaaggaaaga atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac
     8221 gtttgttata aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt
     8281 atctgaaatt gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg
     8341 aaagggtggc atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa
     8401 caacactcac ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag
     8461 aaactgggat ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact
     8521 ctgatgtaaa cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta
     8581 tgtctttagg tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag
     8641 ctgatgcaat ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt
     8701 tgaaatgcta aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct
     8761 agttcaaccg gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc
     8821 tcgtttggat gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc
     8881 agcgcaaagc ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc
     8941 agatccagaa atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga
     9001 atcagcgcta aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa
     9061 tgcttatatg caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca
     9121 cttattaggc gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc
     9181 acatgacttg actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt
     9241 aacagacatt ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc
     9301 agcaatcgtt gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt
     9361 caacgggatc gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga
     9421 aaaagcaggt gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga
     9481 aactgtcaca gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga
     9541 tctagttggt gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct
     9601 gtatatggat tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt
     9661 attagaaggt atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa
     9721 agaactacca tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt
     9781 gcgtatcagt ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct
     9841 tcgtgcatct gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga
     9901 attccgcgca gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa
     9961 ggaagtatct gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt
    10021 agcagacaag tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca
    10081 atacacaatg gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa
    10141 cccatcaatt ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg
    10201 ggcaggtatg tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat
    10261 gggtctagat gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa
    10321 acgcttggac acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat
    10381 gatggaagaa gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct
    10441 ggtgtggctt gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg
    10501 tcagtaagaa tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat
    10561 actatataag tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga
    10621 tgcttgcaaa atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag
    10681 tcggtatctc ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat
    10741 acggaatcaa tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct
    10801 atcatgcgca ttctacagag gaagatttcc gaaattcctt tatcagatcg aaccagcttt
    10861 ctcctatcgt aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc
    10921 caacacctta ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta
    10981 tttccaatgg gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca
    11041 gagaatattt caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg
    11101 aacgtaaagg aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta
    11161 tttggtttgg tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag
    11221 aagatcatcc tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg
    11281 cgtattcagg tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg
    11341 ttttagaagc tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg
    11401 gctggttaga agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac
    11461 tgatccaaca gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag
    11521 caaaagagcg aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac
    11581 tgaatgaaaa agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag
    11641 gcgaaaaaca tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt
    11701 gctacatcga tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt
    11761 tttacaacga ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt
    11821 gtaccattta tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat
    11881 ttggttgcca aagaactaga actagatctt attcataccc atacagaatt cggagcaggg
    11941 atacttggga agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg
    12001 atgtatgaag actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta
    12061 aaatacttgt ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt
    12121 gttattgata cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt
    12181 atcgacattg aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg
    12241 gagtcactag gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat
    12301 gagaaaaata tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac
    12361 gccagattag ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca
    12421 gaactgcagt tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca
    12481 ctttattata aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt
    12541 acttatacag aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg
    12601 aaccgattgt tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca
    12661 ccagcactta ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct
    12721 aaactttatg atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac
    12781 atggttgttt atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga
    12841 atcaaaataa aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc
    12901 cttgtttcta gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa
    12961 aatgaaactt ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact
    13021 agaagcagga cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt
    13081 aacagaagga gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct
    13141 ctttacaatg gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat
    13201 tttttctgca gatatctcag gacatatttt agttgatttg actacaagta caccaagcct
    13261 ggcagaaaaa attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt
    13321 atccggtggt gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga
    13381 agccgtttat gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca
    13441 tggttctgct gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac
    13501 gatgacagga atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa
    13561 agtgattgag acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc
    13621 tcgtatttta aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt
    13681 aaagattgca ttagaagaag cagaaaaaat ggatcttgta ttgccagcaa ctacccaagc
    13741 attgaaacta tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt
    13801 gatcaaactt tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt
    13861 ttttagagaa aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa
    13921 aaggaggacc tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta
    13981 tggtcgagtc agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga
    14041 agtgtacagt aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc
    14101 atcagcaata cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc
    14161 aataaatttt aggataattg acatttgaag ttgaagatgc ctaaaagaag ccgagaagca
    14221 actaatttag ttgcttttca gcttcttttt tgtttaacag ttcaatttca aactgttctt
    14281 tttctaattt tttgcggtaa ctgatcaaga tttgtacagg atcgatttct tcttttgaca
    14341 attgacgttt caaagataaa tgagcttctt tcttttcata atagaagtca ggagaaacgt
    14401 caccagaggt aatcattgtt tctgcaagtt gaccataaca aacgaggtag tgctttttct
    14461 gactctcatc tgctgctaat ggcatgctga aggtgagcga tggtgttctt aaaaactgct
    14521 tttgttcctg gatccgtcta ataaaataag taactaattc gatatctttt tgaagcatac
    14581 cggctttttc gaaattcaac tgatagatga aatgttgttg tctttttttc aagtaagtca
    14641 gtagcttttt atttcggcct tgaaacagcc cttggagttc cttttttttc tgttcaaaag
    14701 tcatttcctg taggtcagga agctggactc gcaaagctaa caaactaatt tcagacaacc
    14761 aaggcatttg ataagtttct tccattatac gcaaaataga cggcattttt ttatagagac
    14821 gaaaaggccc gtatgttcga gccgtaggag tatctgttaa tacaaatccc ttattggtaa
    14881 tatgtacata attataatta ctgaaataat tcatttgtcg gttataaaaa ggacggtaat
    14941 gttggatcaa ttgacactcg agcaataaag catctaattc cgtgtctact acgacgaaat
    15001 caaaatctgt tatatgatga atcatacgta atactttttt tgaatgctgt ttattctgat
    15061 gaaaatagct gcttactcta tttttgagcc gttttgcttt accaacatag atgaccttac
    15121 catctcggtc tttcataaga tagacacccg gcagcatggg gagagttttc attttttctt
    15181 ttaacatagg atctcctttt acgctttgaa taaaaagact ataacaaatt ttgaaataat
    15241 ggacaagttt tagggaattc tatagttctg ttcacagaaa atactttgat aa
//
LOCUS       DACCWV010000066        15281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCWV010000066 DACCWV010000000
VERSION     DACCWV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.066"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            71..481
                     /locus_tag="IVW84_001693"
     CDS             71..481
                     /locus_tag="IVW84_001693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917888.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            562..1182
                     /locus_tag="IVW84_001694"
     CDS             562..1182
                     /locus_tag="IVW84_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917889.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1883..2167
                     /locus_tag="IVW84_001695"
     CDS             1883..2167
                     /locus_tag="IVW84_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7917890.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2170..3087
                     /locus_tag="IVW84_001696"
     CDS             2170..3087
                     /locus_tag="IVW84_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7917891.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            3080..3862
                     /locus_tag="IVW84_001697"
     CDS             3080..3862
                     /locus_tag="IVW84_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7917892.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3863..4543
                     /locus_tag="IVW84_001698"
     CDS             3863..4543
                     /locus_tag="IVW84_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917893.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4536..5432
                     /locus_tag="IVW84_001699"
     CDS             4536..5432
                     /locus_tag="IVW84_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7917894.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5627..7276
                     /locus_tag="IVW84_001700"
     CDS             5627..7276
                     /locus_tag="IVW84_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7917895.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7870..8805
                     /locus_tag="IVW84_001701"
     CDS             7870..8805
                     /locus_tag="IVW84_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917896.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8798..9568
                     /locus_tag="IVW84_001702"
     CDS             8798..9568
                     /locus_tag="IVW84_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917897.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            9984..10967
                     /locus_tag="IVW84_001703"
     CDS             9984..10967
                     /locus_tag="IVW84_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7917898.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            11414..12451
                     /locus_tag="IVW84_001704"
     CDS             11414..12451
                     /locus_tag="IVW84_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7917899.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(12540..12863)
                     /locus_tag="IVW84_001705"
     CDS             complement(12540..12863)
                     /locus_tag="IVW84_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7917900.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(12850..13557)
                     /locus_tag="IVW84_001706"
     CDS             complement(12850..13557)
                     /locus_tag="IVW84_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7917901.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13643..13984
                     /locus_tag="IVW84_001707"
     CDS             13643..13984
                     /locus_tag="IVW84_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7917902.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(14155..15003)
                     /locus_tag="IVW84_001708"
     CDS             complement(14155..15003)
                     /locus_tag="IVW84_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917903.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         4890 a         2604 c         3222 g         4565 t
ORIGIN      
        1 ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg
       61 tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta tttacacaaa
      121 atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta aattatttaa
      181 gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa gaaaagtaag
      241 cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt atggtgatga
      301 tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa gtattcctac
      361 taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg caatttctaa
      421 gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac ctaaaaaata
      481 acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc aatttattca
      541 gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat aggagctaaa
      601 aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa ctggtttttt
      661 tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa aggaatcggc
      721 ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc tgaacaaaca
      781 tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa aaatgtaact
      841 gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca tgaatgtaat
      901 tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg ttgcaaggac
      961 gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc cttatatatg
     1021 tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa aataggtatt
     1081 gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt agaaattttg
     1141 atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt ttttattgag
     1201 atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag gtaatgtttt
     1261 gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac aaagacttat
     1321 taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg tttaaccagg
     1381 tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat tatcgttcga
     1441 gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt accttaatcc
     1501 taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt tagctatgtt
     1561 aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg gaaaaagata
     1621 aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag tggtcagctt
     1681 caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata ttgtgacttg
     1741 tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt gaaaagtctt
     1801 ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt attatttttc
     1861 ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct ggcttcagcg
     1921 agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga ttaccatggt
     1981 ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga caactcaacc
     2041 acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac actttcttta
     2101 tacatttctg gctgctttct ttatacccgt caactatact tcacttatca ataagaaaga
     2161 aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat aaccaaaaaa
     2221 tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg aatgatctat
     2281 ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat tatgggtttg
     2341 atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca ggcagcaaga
     2401 cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac tgcaagagat
     2461 aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga agacctgctg
     2521 aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc tttaggtatg
     2581 cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt gattttggat
     2641 gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat catccaacgt
     2701 ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga tgaattgtca
     2761 cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga attatcgaaa
     2821 gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc gacggaacaa
     2881 gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc tagccgtgtt
     2941 atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt ggtagaggca
     3001 aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta tttcttacaa
     3061 ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa aagtatggaa
     3121 gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt tttattcttg
     3181 gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa cggatacagt
     3241 catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga ttcttctggc
     3301 tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta cgattcgcaa
     3361 tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc tcgttacgct
     3421 tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg cttacacact
     3481 tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg ttctaaaagt
     3541 atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg ctttgacatt
     3601 catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca tgggaacggc
     3661 cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg agtggtttac
     3721 ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc aagcaccgaa
     3781 aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa tgtttatttt
     3841 ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta tcgaagacga
     3901 tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac ttcttgatgc
     3961 ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt tgatccttct
     4021 agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc gacagacatc
     4081 gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag tttccttgtt
     4141 ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac tgttgttacg
     4201 aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt cgcaacgcat
     4261 ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga atggtgtggc
     4321 tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac atccgaaaaa
     4381 agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt ttgatagtga
     4441 gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg cacatgctgt
     4501 gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg ttgcttctcg
     4561 ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag agattaacta
     4621 agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca gtcgaatttc
     4681 gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat gaaaatgaac
     4741 aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc gtcggccttt
     4801 ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta ctggaacagg
     4861 tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct gttgcaagat
     4921 tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag caaaaagaaa
     4981 tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc tctttttatg
     5041 aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata aatacaactg
     5101 tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag aatttattgc
     5161 gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc attcgattaa
     5221 cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc ttccagcgtt
     5281 tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg ctttacctgt
     5341 caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac gggggcatct
     5401 tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg aagtgaatga
     5461 caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt ttattttggt
     5521 gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt tcaaatagcg
     5581 caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg gccaaagaga
     5641 atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag acattttttc
     5701 acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt atcaaccgag
     5761 atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat tccgttcatg
     5821 ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag tatttgaagg
     5881 gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt tacgtagctt
     5941 tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg aaaatcctca
     6001 actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc ataattttgc
     6061 aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt tatttatttt
     6121 tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag tactaggaat
     6181 ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga agaaacaaac
     6241 actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag ccgccaatca
     6301 gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta aaccattaag
     6361 agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag cagaattgat
     6421 ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt tcgttctcac
     6481 taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg gagaattgga
     6541 agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc gaccagagtt
     6601 tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag aagcggtagt
     6661 taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat caggaaaatc
     6721 tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc aaacagcgat
     6781 tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa tcgaagatga
     6841 tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac gtttgctgga
     6901 ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa aaggaacaaa
     6961 tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg agtatccatc
     7021 tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga attattgtga
     7081 gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg atttcaaatt
     7141 aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt tgaattatcc
     7201 taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac agcgtcagtg
     7261 gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa aagacgatta
     7321 tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg agtctgctgt
     7381 ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc tgtcttcctt
     7441 ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc catggcaaag
     7501 ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca ttttatttat
     7561 ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc ttaacatgaa
     7621 taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag tagttgctgt
     7681 cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct atcgaagatg
     7741 atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt gtggaagcca
     7801 agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct ataaggagat
     7861 aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta tggaagtggg
     7921 gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta tgcgctatta
     7981 gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt agtcaataaa
     8041 acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt tcgagcaaag
     8101 caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac ggtccagcaa
     8161 atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat gaaacgcagc
     8221 gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc acgtatgctt
     8281 tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga accacgtttg
     8341 ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga gatgtgggag
     8401 tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca ttatctagaa
     8461 gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact gatcgagaat
     8521 acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt tgatttagcg
     8581 ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga tgagcagaca
     8641 atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca gcttacaaga
     8701 caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga actgttcttg
     8761 aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt attttactgc
     8821 attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg tccaaacact
     8881 ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa tgatcggtgg
     8941 gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg gtttgattat
     9001 gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct ctcaaaaatt
     9061 tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga ttatttgggg
     9121 ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa cgatcatttc
     9181 cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta cattattgat
     9241 gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc agtcttatga
     9301 tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag gtggcgtttt
     9361 ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca atccgatcgt
     9421 ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc cagtagcaac
     9481 atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt ggtatttgat
     9541 caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg ttttgacctg
     9601 agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt tgagcgaata
     9661 ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc tgttctgcat
     9721 caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gttggactta
     9781 ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaatggactg tgataagatt
     9841 tttgtcacag tctttttttt gctcaatcag aaacatcttt taagctgttc actttgcttc
     9901 tttttttgtt tcctagtata atgacaatgt ttttgagaaa gattgcgact tcactgcttg
     9961 atagaatgga gagtaacaaa taaatgacta ctaatttttg gaaagagctg ccaaagcctt
    10021 tttttgtttt agcaccaatg gaagatgtaa cagatgttgt atttcgccat gtcgtaaagc
    10081 atgcagcagc accggatgtc ttctttactg agtttacaaa ctcagacagt ttctgtcatc
    10141 cagatggaaa agacagtgta cgaggaagat taacatttac agaagatgaa cagccgatgg
    10201 ttgcccatat ttggggagac aagccagaat tttttagaga aatgagcatt gctatggcag
    10261 aaatgggctt ccaaggaatc gatatcaaca tgggctgccc agtccctaat gtagcagaac
    10321 gtggaaaagg cagtggcttg atccttcgtc cagaagtagc ggcacaattg attgaagcag
    10381 ctaaagctgg cggtttgcct gtcagcgtca agacgcgtat cggctatacg gaaatgtctg
    10441 agatggaaga atggatcagt cacctacttc atcaagatat agccaatctt tctgtccatt
    10501 tgcgtacgcg taaagagatg agtaaagtag atgcacattg ggaagtcatt cctaaaatca
    10561 ttgctttacg tgaccaaatc gctccacaaa cactaatcac gatcaatgga gatatccccg
    10621 atcgccagac aggagaagaa ttggccgaga aatatggcgt tgatggcatc atgattggcc
    10681 gagggatttt caagaatcct tatgcatttg aaaaagaccc gagggaacat acagaaaaag
    10741 aactgatcgg actacttcgt ctgcaactag atctgcaaga tcattattca gaaatcatcc
    10801 cgcgttccat cacaggacta catcgtttct ttaaaatcta tatcaaagga tttccaggag
    10861 caaatgactt gcgagttcag ttgatgaata cgaaatctac tgatgaagta cgagagatct
    10921 tagagacctt tgaaaaagaa catccaacgc ttttcagtga acaatgattt cttgcttgaa
    10981 caccgtgaat tagaggtcgt gaaaaagctg tcctgtatca agtaataaga acagccaaac
    11041 aaaaatagct cttcatattt tgtcgttttt tgagctaata ccgagggtca gttgattgaa
    11101 caccgtttat atcagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggagcaattt
    11161 caaaaaacag cttctcatgt ttgatgaata ttttgacttg tgaccgaaga gttggtagga
    11221 gaacaccgtt tataagaggt tgtgacaaaa gtcttgtcgc aacctctttt ttaaccaaaa
    11281 aagtgcaaat agtttacttt aagtaagaga ataattatga tgaataaaaa aggtggaaca
    11341 aatagcatac tatatatatt gaaatgaaca ctgtatggat gctccaactt gatgaagcta
    11401 aggaggaatt cgtatgtcag aaaaggaatt aatgaaggta gtcggttttt atgaagggtt
    11461 gccgatcgat gatccaaaaa gctttattga tgaaaaagaa cagatacctg tacccagttc
    11521 acgggatctt ctggtaaaag tcaacgcagt atctgttaat cctgtcgata cgaaacttcg
    11581 ccaagataac gggataagaa atgctttacg tatcttaggt tttgatggag tcgggaaagt
    11641 tgttgctgta ggtgacgaag tgcaaaaatt ttctgtcggc gatcgcgttt tttacgcagg
    11701 tacaacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac gtattgtcgc
    11761 tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga cctcattgac
    11821 cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg ccaatcgcgg
    11881 gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga atcaactagc
    11941 gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg aatggctgaa
    12001 aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa gttttcgtga
    12061 attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc gctactttga
    12121 tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag ggatcaaaga
    12181 gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg agtatatgtt
    12241 cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag cgcacattgc
    12301 tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt cagatgggat
    12361 gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata tgttaggaaa
    12421 agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt tttcttattt
    12481 gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg tttttttatt
    12541 tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac caataagctt
    12601 tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc gagattgatc
    12661 tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc agacataatg
    12721 acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa ttttttcagt
    12781 aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa aatagtcagc
    12841 aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc caatcagtgt
    12901 tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata ccagaatcaa
    12961 agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag aaatgacttg
    13021 aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa actttttcgg
    13081 atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc acgtcaaata
    13141 agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag tatagttcaa
    13201 tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca gcagattttg
    13261 aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa tcattcggga
    13321 attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag caagcatgct
    13381 gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg tgacgactag
    13441 tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac cgatataacc
    13501 gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt catccaataa
    13561 caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt gaatattcta
    13621 aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag cgggtcgcag
    13681 atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc tacaaatcca
    13741 agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac ctgcattagg
    13801 cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa gttgttgtca
    13861 gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag aaaagtatca
    13921 taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt gtcatgactt
    13981 ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg ttaagtcttc
    14041 tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta tttcttgtgg
    14101 atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct ttctttaccg
    14161 tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt cccgaaagtc
    14221 ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat gatcgacttg
    14281 gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg ctaataattc
    14341 tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct caggctccca
    14401 atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg gaagataggc
    14461 attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct ccatgatgaa
    14521 cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat ggatgatagt
    14581 tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga agcgttcgat
    14641 aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag aggaactagc
    14701 ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta ttggacgttc
    14761 ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta tggcatatag
    14821 acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat aatcttccgt
    14881 gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga caaaaccgtg
    14941 cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa gccaattatt
    15001 catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa cgtttaagaa
    15061 aaaagtagct gattttacat atttgataat cacttttgct cgaattgtca aaaattcatt
    15121 ctccttttct tttcttagag aatgtgagaa aaagtaaaag aatagcgtgt tttaaagatg
    15181 gaactttttt tgttttttat ttatctgaca aaaatgaagt gattttgtta gaaataaata
    15241 attgttccaa ttgataaaag aggtatgatg tatcgcaaag a
//
LOCUS       DACCWV010000067        14815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCWV010000067 DACCWV010000000
VERSION     DACCWV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.067"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(296..1024)
                     /locus_tag="IVW84_001709"
     CDS             complement(296..1024)
                     /locus_tag="IVW84_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7917904.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVW84_001710"
     CDS             complement(1002..2576)
                     /locus_tag="IVW84_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7917905.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVW84_001711"
     CDS             2805..3743
                     /locus_tag="IVW84_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7917906.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVW84_001712"
     CDS             3755..4666
                     /locus_tag="IVW84_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7917907.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVW84_001713"
     CDS             4690..6162
                     /locus_tag="IVW84_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7917908.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IVW84_001714"
     CDS             6213..6824
                     /locus_tag="IVW84_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7917909.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IVW84_001715"
     CDS             6854..8767
                     /locus_tag="IVW84_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP7917910.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IVW84_001716"
     CDS             8895..11876
                     /locus_tag="IVW84_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP7917911.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IVW84_001717"
     CDS             11873..13534
                     /locus_tag="IVW84_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP7917912.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IVW84_001718"
     CDS             13574..14506
                     /locus_tag="IVW84_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7917913.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2334 c         2978 g         4688 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DACCWV010000068        14806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCWV010000068 DACCWV010000000
VERSION     DACCWV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.068"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(285..851)
                     /locus_tag="IVW84_001719"
     CDS             complement(285..851)
                     /locus_tag="IVW84_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7917914.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(851..1807)
                     /locus_tag="IVW84_001720"
     CDS             complement(851..1807)
                     /locus_tag="IVW84_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7917915.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1807..2463)
                     /locus_tag="IVW84_001721"
     CDS             complement(1807..2463)
                     /locus_tag="IVW84_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7917916.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2833..4851
                     /locus_tag="IVW84_001722"
     CDS             2833..4851
                     /locus_tag="IVW84_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7917917.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5278..7692
                     /locus_tag="IVW84_001723"
     CDS             5278..7692
                     /locus_tag="IVW84_001723"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7917918.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8040..9593
                     /locus_tag="IVW84_001724"
     CDS             8040..9593
                     /locus_tag="IVW84_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7917919.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9974..11758)
                     /locus_tag="IVW84_001725"
     CDS             complement(9974..11758)
                     /locus_tag="IVW84_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917920.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11773..13494)
                     /locus_tag="IVW84_001726"
     CDS             complement(11773..13494)
                     /locus_tag="IVW84_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917921.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13594..14355
                     /locus_tag="IVW84_001727"
     CDS             13594..14355
                     /locus_tag="IVW84_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7917922.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4813 a         2725 c         2938 g         4330 t
ORIGIN      
        1 atatcaatgt ttttgccatt cttttatata tctgttatat gtcaaaaaaa tgtgttcaat
       61 ttcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
      121 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
      181 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
      241 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
      301 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
      361 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
      421 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
      481 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
      541 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
      601 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
      661 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
      721 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
      781 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
      841 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
      901 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
      961 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     1021 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     1081 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     1141 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     1201 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     1261 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     1321 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     1381 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
     1441 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
     1501 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
     1561 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
     1621 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
     1681 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
     1741 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
     1801 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
     1861 cactggataa gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt
     1921 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
     1981 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
     2041 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
     2101 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
     2161 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
     2221 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
     2281 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
     2341 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
     2401 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
     2461 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
     2521 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
     2581 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
     2641 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
     2701 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
     2761 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
     2821 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
     2881 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
     2941 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
     3001 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
     3061 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
     3121 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
     3181 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
     3241 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
     3301 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
     3361 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
     3421 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
     3481 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
     3541 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
     3601 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
     3661 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
     3721 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaac
     3781 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
     3841 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
     3901 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
     3961 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
     4021 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
     4081 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
     4141 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
     4201 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
     4261 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
     4321 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
     4381 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
     4441 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
     4501 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
     4561 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
     4621 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
     4681 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
     4741 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
     4801 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
     4861 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
     4921 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
     4981 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
     5041 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
     5101 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
     5161 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
     5221 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
     5281 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
     5341 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
     5401 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
     5461 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
     5521 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
     5581 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
     5641 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
     5701 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
     5761 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
     5821 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
     5881 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
     5941 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
     6001 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
     6061 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
     6121 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
     6181 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
     6241 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
     6301 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
     6361 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
     6421 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
     6481 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
     6541 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
     6601 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
     6661 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
     6721 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
     6781 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
     6841 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
     6901 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
     6961 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
     7021 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
     7081 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
     7141 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
     7201 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
     7261 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
     7321 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
     7381 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
     7441 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
     7501 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
     7561 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
     7621 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
     7681 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
     7741 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
     7801 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
     7861 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
     7921 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
     7981 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
     8041 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
     8101 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
     8161 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
     8221 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
     8281 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
     8341 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
     8401 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
     8461 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
     8521 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
     8581 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
     8641 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
     8701 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
     8761 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
     8821 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
     8881 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
     8941 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
     9001 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
     9061 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
     9121 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
     9181 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
     9241 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
     9301 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
     9361 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
     9421 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
     9481 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
     9541 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
     9601 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
     9661 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
     9721 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
     9781 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
     9841 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
     9901 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
     9961 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    10021 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    10081 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    10141 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    10201 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    10261 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    10321 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    10381 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    10441 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    10501 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    10561 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    10621 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    10681 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    10741 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    10801 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    10861 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    10921 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    10981 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    11041 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    11101 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    11161 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    11221 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    11281 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    11341 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    11401 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    11461 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    11521 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    11581 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    11641 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    11701 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    11761 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    11821 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    11881 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    11941 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    12001 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    12061 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    12121 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    12181 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    12241 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    12301 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    12361 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    12421 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    12481 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    12541 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    12601 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    12661 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    12721 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    12781 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    12841 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    12901 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    12961 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    13021 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    13081 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    13141 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    13201 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    13261 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    13321 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    13381 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    13441 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    13501 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    13561 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    13621 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    13681 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    13741 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    13801 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    13861 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    13921 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    13981 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    14041 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    14101 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    14161 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    14221 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    14281 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    14341 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    14401 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    14461 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    14521 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    14581 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    14641 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    14701 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    14761 gaataaacat attttttaat caaatagtcc ctactgtgac tttcct
//
LOCUS       DACCWV010000069        14750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCWV010000069 DACCWV010000000
VERSION     DACCWV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.069"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            10..1749
                     /gene="yfcC"
                     /locus_tag="IVW84_001728"
     CDS             10..1749
                     /gene="yfcC"
                     /locus_tag="IVW84_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7917923.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1906..3090
                     /gene="iadA"
                     /locus_tag="IVW84_001729"
     CDS             1906..3090
                     /gene="iadA"
                     /locus_tag="IVW84_001729"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7917924.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3201..3404
                     /locus_tag="IVW84_001730"
     CDS             3201..3404
                     /locus_tag="IVW84_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7917925.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3505..4167)
                     /locus_tag="IVW84_001731"
     CDS             complement(3505..4167)
                     /locus_tag="IVW84_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7917926.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4180..5682)
                     /locus_tag="IVW84_001732"
     CDS             complement(4180..5682)
                     /locus_tag="IVW84_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7917927.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6230..7909
                     /locus_tag="IVW84_001733"
     CDS             6230..7909
                     /locus_tag="IVW84_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7917928.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8036..8515
                     /locus_tag="IVW84_001734"
     CDS             8036..8515
                     /locus_tag="IVW84_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7917929.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8734..9414
                     /locus_tag="IVW84_001735"
     CDS             8734..9414
                     /locus_tag="IVW84_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7917930.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9422..10753
                     /locus_tag="IVW84_001736"
     CDS             9422..10753
                     /locus_tag="IVW84_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7917931.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10768..11730
                     /locus_tag="IVW84_001737"
     CDS             10768..11730
                     /locus_tag="IVW84_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7917932.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11841..12179
                     /locus_tag="IVW84_001738"
     CDS             11841..12179
                     /locus_tag="IVW84_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7917933.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12258..12965
                     /locus_tag="IVW84_001739"
     CDS             12258..12965
                     /locus_tag="IVW84_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7917934.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13068..14549)
                     /locus_tag="IVW84_001740"
     CDS             complement(13068..14549)
                     /locus_tag="IVW84_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7917935.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4664 a         2543 c         3169 g         4374 t
ORIGIN      
        1 gaaataaatt tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta
       61 tcgacacccc atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact
      121 tttgtcgttc cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc
      181 gaaacaagta ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat
      241 aagtcgtatg tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa
      301 ctggatgttc cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg
      361 caggaaaaac tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa
      421 aatatctatg atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat
      481 tttggcggct ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat
      541 ggatctgctg tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt
      601 atgcgtaccg gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg
      661 gagcgtttag ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg
      721 atggcagaag aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga
      781 tatgactcga tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc
      841 tcttggatga gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg
      901 tctggtgcga cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat
      961 atgatgatat atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca
     1021 gatgattatt tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt
     1081 gggcataaat tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg
     1141 actcaaaaag gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt
     1201 gctggaatca ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct
     1261 tctttccaat ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa
     1321 gggattcttc ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt
     1381 ttgcatggga tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg
     1441 tacatcttcc aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg
     1501 actatgccaa tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt
     1561 cttgcttatc agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg
     1621 attggtgtgc taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag
     1681 atgcaaggat tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt
     1741 ctgcagtagt actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc
     1801 aagaaccgtc attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt
     1861 ttaagatcct atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga
     1921 caaattgaag tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg
     1981 aagattttgg ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa
     2041 ctttcaggtg agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta
     2101 ggtggaggag gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg
     2161 actacagctg gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat
     2221 atgactgcgt tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta
     2281 tacgaaggtt cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt
     2341 ttgacaattg ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct
     2401 cagccaagtt ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta
     2461 tccggaaaag ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta
     2521 ttgacacgaa tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca
     2581 aatcgcacac cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt
     2641 gacttcactg ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc
     2701 agcaaagcac tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc
     2761 tcagatggac aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga
     2821 gtaggaagtg ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc
     2881 cctttggaaa tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat
     2941 aaaaaaggaa aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg
     3001 cttgatattg atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa
     3061 gtatggggga ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa
     3121 cgagcgttct gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca
     3181 aactaagagg aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata
     3241 aagtagtcgg tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa
     3301 cagaagggaa agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg
     3361 acgaagtcaa agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta
     3421 attgaatcaa gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt
     3481 tttatttgat cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag
     3541 ttcaaagact tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca
     3601 gcgattttgt tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc
     3661 atctagattt ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg
     3721 aacgatagcg actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa
     3781 tatttcatca attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg
     3841 cttggtctta gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag
     3901 caagttgtaa gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat
     3961 tttgttcagt gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga
     4021 aataagcgaa agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt
     4081 tccagcttcg aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca
     4141 aaatcccaat tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct
     4201 tgggtttaat cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa
     4261 tgctactccg gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac
     4321 ttgatcagaa acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc
     4381 ttgttcttgt ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt
     4441 ggtttctgtc tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct
     4501 aagcgaagca actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag
     4561 aatgatttta cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc
     4621 ccgaatggta atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa
     4681 aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat
     4741 ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag
     4801 aaactcatcc attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc
     4861 gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg
     4921 attcatgaag ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc
     4981 tttgatcgtc atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc
     5041 tccgactgcc aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc
     5101 tttgtcgctg tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt
     5161 actattttgt gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg
     5221 attcccgccc attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc
     5281 acttgaatca gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact
     5341 ttcaaaggaa taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga
     5401 aactggtgta agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga
     5461 agaagtttgt tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc
     5521 tccaacttct ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc
     5581 agctagcaca aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca
     5641 ttttactttt gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag
     5701 tagatgcatt tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc
     5761 acaccatcaa catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt
     5821 ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa
     5881 agaaatccta gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat
     5941 actagcgatt tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt
     6001 attctggcta tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa
     6061 atgcccatcc aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa
     6121 gttgagaaat agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga
     6181 agaaaatcga catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat
     6241 taaaattatc ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt
     6301 gggcgaggat atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg
     6361 gatcgacgtc gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg
     6421 tgtcttttta acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa
     6481 aatttctgta ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag
     6541 tcgtaatgaa gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat
     6601 agactttggt gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt
     6661 ggggatcaat ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga
     6721 ccaaacggct atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga
     6781 aggagtctta gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc
     6841 tgaagcccaa atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat
     6901 cgtcgcttgt gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa
     6961 agcacaacga aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat
     7021 gcgtttagaa aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa
     7081 aaactatgat ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa
     7141 gtctctgcaa aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt
     7201 ggtttatatc actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga
     7261 tatcatttat cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg
     7321 ccatgccaat cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt
     7381 gccagttcaa ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg
     7441 gatgccatac aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa
     7501 gcgtatgagt gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat
     7561 aggtgatatc ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt
     7621 cgtagcggtt gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac
     7681 ttcacgggga ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat
     7741 gattgaaaca atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa
     7801 gcaagacatc cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt
     7861 gatcttgcca gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt
     7921 aagatgaacc tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt
     7981 ttgaatacag catccctcgc tttataataa acgatagaat aaagggagtg aatcaatgat
     8041 gagtcaaaca aaagtcgaac gttatttaga agaacaaaag atcagctatc aaccacttga
     8101 ttttagtcat gtggtagaag aatctttcac agaagaacta caaaaaagag ggattgatcc
     8161 agctttgata tgtaaaactc tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt
     8221 gattcctttg aacgatcact tagattacaa aaaaacgaga caagtgacga aagatcgtaa
     8281 agttggtttt cctggaatgg attttgtatt aatgcataca ggatatcccc acggagcaaa
     8341 cacccctatt ggaataaaac tcgcacatcc tgattattgt tttttagcag acagttcttt
     8401 aaaagaaaaa gaagaagtaa ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa
     8461 agtagccgat ttagaaaaga tcgtccatcc tatttacgct gatttaatta gttaagggac
     8521 gagaagagaa gctaagcaga gcagagacct caaacttgag attatatctg cttggtactt
     8581 ggtgcatcct gtattatctg ttacaattgc tttgtacata ctgtactatc aatccaatga
     8641 aaatatattt agcagctata ttttttcatt gatgtgcaag tgatgaaatc gtttttaata
     8701 aaaaagatac agagaaaacg cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc
     8761 tgtttaacaa ttgaaatgga aaatggccaa cgcgtgatga ttgacccgtt tattacagga
     8821 aatccattga cagacttaac tgcagaagaa gtaaagacag attggttgct gattactcat
     8881 ggtcatagtg accacatagg agatatgatt cctattgcaa aagaaaatca agccacaatt
     8941 atcagtattg ttgagattgc tgattatgcg aacagcagag gagtagattc attcggtatg
     9001 aatatcggtg gaaaacatgc gtttccattc ggcgtggtaa aattcgtaca tgcacagcat
     9061 agttcaagct atgaagtaga cggtatcgtt cagtatatgg gcgagcctag cggaattatt
     9121 attcaagcag aaggaaaaac gatttatcat gcaggggata ctgcttattt cagtgatcta
     9181 ggattgcttg cagaagaatt cgatattgat gtcgctttct tgccgattgg cgataactat
     9241 acgatggggc cagaagatgc agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt
     9301 ccaatccatt acaatacttt ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta
     9361 ctggaagata gtgaaggaaa aataatgagc gtctcggaaa cgctggaagt gtaggcagaa
     9421 aatggcgaca aaacatgatt tgattttaga atatattgaa agtttacctg ttggcaatcg
     9481 aatttctgta agaagcatcg caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc
     9541 aatcaaagat gcagaaaatg tcggactagt ttcaacaatt caacgagtag ggaccatccg
     9601 tatcgaacgc aaattaaaaa aacatattga acgtttgaca tttggcgagg tcgtcaggat
     9661 cattgaaggg gacgttttag gcggggcggc aggattagac aaggtcttga ataaattcgt
     9721 catcggcgcg atgactaaac atgccatgga acgctatatt acgccaggat cactaatgat
     9781 cgtcggcaac cgggcggaag tccaaaaatt agcattggaa gatggcgccg cagtattgat
     9841 cacaggagga ttcaatacga ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc
     9901 gatcttacgc acctcttatg atacatttac agtcgcaacc atgatcaacc gagcgttaag
     9961 cgaccaattg atcaaaaaag atatcatgct tgtcagtgat atctatatgc cagctgaaaa
    10021 gactcattat ttgcagcaga cagatacgat ttctgattat cagcgtttgt cagaagaaac
    10081 gcagcattcg cgttttccag tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc
    10141 aaaagatgtt ttagggaaat cgccaaacca gatcatcgat cgggtgatga cgaaagaacc
    10201 aatcagtgta aagaaaacaa tgagtattgc ctctgttagc catcaaatga tctgggatgg
    10261 gctagaagtc atgccggtcg tttccgatga cttgacattg gaaggcttga ttacccggca
    10321 agatgtaatg aaagcgatgc agcttgtcca gcgacaaccg cagattgcag atacgatttc
    10381 ggatcagata tctggaaata tccatacgat tgatactgac cgtgaaggaa atcgtttgga
    10441 aaaccctaag ttcaaattcg tagtagcacc acaaatggtc aatggcgttg gaaccatttc
    10501 ttttggcgta cttagcgaaa tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca
    10561 aaaacgaaat atcttgattg aacaagtgaa cctacattat ttgcggctga tccaattgga
    10621 aagcgaatta gacattcgtc cacgtgtatt ggaaatcggc agacgttccg ctaaactgga
    10681 tatcgaagtg tttattgaaa acaccatcgt ttccaaagca atcgttgttt gtcaagtaat
    10741 ggaacgttct tagaaagcag gagtgaaatg acagtacagc aagaaatagt agatacaata
    10801 aaaaaatacg ataaaatcat tatccacagg catttgcgcc ctgatcctga tgcattagga
    10861 tcacaagtag gattagccga actattacgg gaaagtttcc cagaaaaaga aatacgtcaa
    10921 gtaggtgaaa cgattgaagg gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc
    10981 ttctaccaag gagcgctagt catcgtgact gatacagcta attcacccag aatcagtgat
    11041 caaagatatc agttggcaga aaaactgatc aaaatcgacc atcatcccaa tgatgaccct
    11101 tatggagatc ttgtgtgggt aaatacaaaa gcaagtagct gtagtgagat gattgcagaa
    11161 tttgctttga tgtttcctga atctttaaag atgaacgtta aggctgcacg gttactttat
    11221 gcaggaatcg tcggagatac agggcgcttt ttgtatccct ccacgacgtc ccgtacatta
    11281 gagattgctt cgatattgag aaattatcca tttgatgcaa gtgctttgaa cagagagatt
    11341 gaacaaatgc cgatgaaagt cgctaaactt tccggttatc tttatcaaaa tatccaggtg
    11401 gatgaaaatg gtgcaggaag aataatcctt ccgcaagaaa ttcttgataa atatggaatc
    11461 gatgattcag agacagcttc gatcgtttca cttcctggag tgattgacga agtgctggct
    11521 tgggggattt ttgttcagca gccttcaggg tattaccgcg tacgtttacg ttctaagggg
    11581 ccgatcatca atgaattggc taaacgccat catggaggag gccatccatt agcaagtggt
    11641 gcgaatgcaa aagatttagc agaagtagag gaaatctata aagagatcca agatatatgc
    11701 caatcgtata agagcagaaa aacagaatga tacttcgttc gaaaacgtgc cttttgtagt
    11761 aaaaatcaca aatgtatctt ttcaagatct tctttttccg ttacaatagt gctcactaaa
    11821 gattttaaag gagaaagaag atgacattac caaattgccc agaatgtggc tcagagtaca
    11881 catatgaaga tcgaggcttg ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag
    11941 aacaagaaac tgtcatagaa gacggattag tggtattaga tgcaaatgga aaccgtctgt
    12001 tagagggcga tagcgtgaca gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca
    12061 ttaaacaagg aacaaaagtc aagaacatcc gtttagtaga aggcgatcat aatatcgatt
    12121 gcaaggtaga aggatttgga ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa
    12181 gaatattcaa ggtcttttca cattcttttc ctactactaa tgataaaata aagtaaacga
    12241 aaaggagtga aacgaaaatg tatggttatg gaatgtatgg ttttttagat ccgacatttc
    12301 ttttggtcat tcttggatta gttatttccg gaattgcgtc agcgtacgtc aatagtactt
    12361 tccgaaaata cgatcaagtg agaagcaaaa acaatgtgac tggtacacaa gcagcacaat
    12421 atatattaca aagtcaacag ataaataatg tcggtgttca acaaattgcc ggagatctca
    12481 cagataacta caattcaggt aataagatgt tgagtttgtc tcaagcaact gcacagtcta
    12541 cttcagttgc tgcgataggt gtagcagctc atgaatgcgg acatgcggta caagatgctt
    12601 cgggttatgt cccattgaag attcgtgcag cacttgttcc agcagctaac tttggttcga
    12661 tgatttcgtt ccccttgatt atggtaggtg tattgcttag ctggaactct acgttaatca
    12721 atatcgggat tttcgctttt tctttagcat tacttttcca attggtgaca ttaccagtcg
    12781 aatttaacgc gtctcggcga gcaatccaaa ttttgagtga aggcggacta ctgacggaag
    12841 aagaagtacc gatggcaaga cacgtattat ttgcagcagc actgacttat gttgcagcag
    12901 cgctttcgac attcctgcag ctgttgcgct tgatcttatt gttcggcggc aaccgaagag
    12961 attaaaataa agaagttgtg aaatcagcgc tttgatctga gctaataccg agagttagct
    13021 gattgaatac catttatttg gaataagagg atgtgacaag aattttgtca catcctcttt
    13081 gttttgttct cataaccttt tttcatatac agttcatgaa ccgcttttat taacttgcca
    13141 aataacactg tattgtgcgt tgtatccaga tagaaagtct ccgtttcatc tgaaattttc
    13201 tcatgctgat ctgtaatgat caaatcagca tgttctgaat atgcggtaat tttaacagca
    13261 tctttgcgaa ataactgctg caaatggctt tgaagatgat aatttcctgt aaactgtttg
    13321 gttatctgca aatggatgag taactgctct ttcgtttctg tttgtagtaa gtgttggatc
    13381 aattgactaa ggtattgact gatttggttt ctttccccat tttttgaaaa aattgtatcg
    13441 attttttctc ttaatttctt ttcctgtata aaagaactag aatgattgga cgtggcttct
    13501 ggaaagcaaa gtgtagaaaa actactgctg gattcaccca acagttcgaa aaaaacaact
    13561 gccaacacaa gaaaataaaa ataaattggt attttatttt ctaaatggtt gagaggaaac
    13621 tgttgagtta agtaagtcat caattgaccg acttgtcgac aaataaggtt tttgcttgtg
    13681 gaaaataaaa aaccaatctc ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa
    13741 tagaaaaaat aattaaagta ggctctctct gtttctgtct cttcacaagg gcgactcact
    13801 gaaaaagaag taaagtattc tgaataatca tgtgtatgct gcaataagtc catcaattca
    13861 gtgatttcag aagaagtttt tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa
    13921 gttaacagca agtagatatt tttttcttgt tttgaaattt tttctatttg ctcatctgta
    13981 ctgttcattt ttagtgcatt taattcaagt gaggaaaatg gccaatcgtt acctccaaat
    14041 gtatctgaaa gaaaaaagta aagatagaga cggatagata actcatttcc ttcaagagta
    14101 cagcaatttt ttacagatag gatccgtaaa tggaatttta ttaaggaatg attgagctgt
    14161 tttgtcagtt tttcgagata ggagggcgat atataaagtt gttcaagtaa atattttttt
    14221 ggtacagcag tttgtggata attgattagt aacatcaaat agttaaaacg attggattct
    14281 tttaggtagc ataattttaa cttagaaaaa acagctaact ttttagtttg atcggtttct
    14341 ttggtttgga tctctccttt atgattagaa taaataaatt catcaatcaa taaaaattgc
    14401 ttgagtgata catttatttt tcggatcagt tctttgattg tccgttttgg aatatgtaag
    14461 tcattggata attcttgtaa agaacaatta tttttttgaa tgaccatttg tagaacaagt
    14521 aactcttttt ttcttgcttc aattagcata aatacctcct ttttattctg tttttgtata
    14581 tagttagaca aaaactgtga gtggaaagtc atttcagtct gtgttattct cacagagtgt
    14641 cgccttcttt tttatataac gttatttaat ttttttgtta ccttgtttat tctattgttt
    14701 atttcctagg gtattttgtc aagatattct tgtttttaag gcggaaaaat
//
LOCUS       DACCWV010000070        14476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCWV010000070 DACCWV010000000
VERSION     DACCWV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.070"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..601)
                     /locus_tag="IVW84_001741"
     CDS             complement(26..601)
                     /locus_tag="IVW84_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7917936.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="IVW84_001742"
     CDS             complement(606..1580)
                     /locus_tag="IVW84_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP7917937.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="IVW84_001743"
     CDS             1863..2831
                     /locus_tag="IVW84_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7917938.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="IVW84_001744"
     CDS             2928..3221
                     /locus_tag="IVW84_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7917939.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="IVW84_001745"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="IVW84_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7917940.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3687..4433
                     /locus_tag="IVW84_001746"
     CDS             3687..4433
                     /locus_tag="IVW84_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7917941.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="IVW84_001747"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="IVW84_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7917942.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="IVW84_001748"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="IVW84_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7917943.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="IVW84_001749"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="IVW84_001749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7917944.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="IVW84_001750"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="IVW84_001750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7917945.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="IVW84_001751"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="IVW84_001751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7917946.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="IVW84_001752"
     CDS             9170..9712
                     /locus_tag="IVW84_001752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7917947.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="IVW84_001753"
     CDS             9735..11291
                     /locus_tag="IVW84_001753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7917948.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="IVW84_001754"
     CDS             11305..12207
                     /locus_tag="IVW84_001754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7917949.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="IVW84_001755"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="IVW84_001755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7917950.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="IVW84_001756"
     CDS             13650..14072
                     /locus_tag="IVW84_001756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7917951.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4780 a         2608 c         3113 g         3975 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt cctttt
//
LOCUS       DACCWV010000071        14292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCWV010000071 DACCWV010000000
VERSION     DACCWV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.071"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..586
                     /locus_tag="IVW84_001757"
     CDS             <1..586
                     /locus_tag="IVW84_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7917952.1"
                     /translation="GKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEFGHLEGDTIV
                     GIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKE
                     FSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRKVNQTFISSV
                     SNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1092..1694
                     /locus_tag="IVW84_001758"
     CDS             1092..1694
                     /locus_tag="IVW84_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7917953.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1710..2387
                     /locus_tag="IVW84_001759"
                     /pseudo
     CDS             1710..2387
                     /locus_tag="IVW84_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2899..3195
                     /locus_tag="IVW84_001760"
     CDS             2899..3195
                     /locus_tag="IVW84_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7917954.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3196..3612
                     /locus_tag="IVW84_001761"
     CDS             3196..3612
                     /locus_tag="IVW84_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7917955.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3632..5176
                     /locus_tag="IVW84_001762"
     CDS             3632..5176
                     /locus_tag="IVW84_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7917956.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5319..5543
                     /locus_tag="IVW84_001763"
     CDS             5319..5543
                     /locus_tag="IVW84_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7917957.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5558..6427
                     /locus_tag="IVW84_001764"
     CDS             5558..6427
                     /locus_tag="IVW84_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7917958.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6408..7142
                     /locus_tag="IVW84_001765"
     CDS             6408..7142
                     /locus_tag="IVW84_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7917959.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7175..8083
                     /locus_tag="IVW84_001766"
     CDS             7175..8083
                     /locus_tag="IVW84_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7917960.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8080..8622
                     /gene="sat4"
                     /locus_tag="IVW84_001767"
     CDS             8080..8622
                     /gene="sat4"
                     /locus_tag="IVW84_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7917961.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8715..9509
                     /locus_tag="IVW84_001768"
                     /note="aph(3')-IIIa"
     CDS             8715..9509
                     /locus_tag="IVW84_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7917962.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9617..9805)
                     /locus_tag="IVW84_001769"
                     /pseudo
     CDS             complement(9617..9805)
                     /locus_tag="IVW84_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9785..10357
                     /locus_tag="IVW84_001770"
     CDS             9785..10357
                     /locus_tag="IVW84_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7917963.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10697..11551
                     /locus_tag="IVW84_001771"
     CDS             10697..11551
                     /locus_tag="IVW84_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7917964.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11650..11859
                     /locus_tag="IVW84_001772"
     CDS             11650..11859
                     /locus_tag="IVW84_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7917965.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11876..12148
                     /locus_tag="IVW84_001773"
     CDS             11876..12148
                     /locus_tag="IVW84_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7917966.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12150..13013
                     /locus_tag="IVW84_001774"
     CDS             12150..13013
                     /locus_tag="IVW84_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7917967.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13276..14013)
                     /gene="erm(B)"
                     /locus_tag="IVW84_001775"
     CDS             complement(13276..14013)
                     /gene="erm(B)"
                     /locus_tag="IVW84_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7917968.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14138..14221)
                     /locus_tag="IVW84_001776"
     CDS             complement(14138..14221)
                     /locus_tag="IVW84_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7917969.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5058 a         2243 c         2948 g         4043 t
ORIGIN      
        1 aggtaaaaga aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc
       61 aatccatgat cgatctgata attatcctga tttcaattct gagtttggtc accttgaagg
      121 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
      181 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
      241 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
      301 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
      361 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
      421 taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt
      481 cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat
      541 atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa tcatactatg
      601 atacgtgttt gtttttctgt gctgtttagt gaatgattag cagaaatata gagagtaaga
      661 ttttaattaa ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg
      721 actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac
      781 acttgatctt ttaagttgct atctttttaa ggtcaataga gtatacttat ttgtcctaat
      841 atggttttag cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag
      901 gagtaaaatg aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt
      961 taatgagcaa caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca
     1021 atttgaaatt gaaaaagtag atatagctga ttggttattg caaccgagag aattttagag
     1081 gttggttgaa aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag
     1141 atagacaact gaaagcgtta gagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc
     1201 aatcggtcga acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatatag
     1261 ttgttgttac tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga
     1321 atcaaattca aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta
     1381 ttgaagatga aaatttaaga cggctgatta ataatttagt gattgaattg tataagtacc
     1441 aagcggaatc tgaacgcaaa cgaattaaag aacgccaagc ccaaggaatt gaaattgcta
     1501 agaaaaaagg aaaattcaaa gggcgacaac ctaaattcaa agaaaatgat ccacgtttac
     1561 aacacgcttt cgatttattt ttgaatggtt gttccgataa agaagttgaa gaacaaactg
     1621 gaattaatcg ccgaacgttt agaaggtatc gatcaagata caacgtgaca gtagatcaaa
     1681 gaaaaaaaca atgaaaagag ggatagttaa tgagtacggt tattttagct gaaaaaccaa
     1741 gccaggcatt agcctacgca agtgctttaa aacaaagcac caaaaaagac ggttattttg
     1801 agatcaaaga cccactattt acagatgaaa cgtttatcac ctttggtttt gggcatttag
     1861 tcgaattagc agaaccaggt cattataacg aaaagtggca gaactggaaa cttgaatctt
     1921 tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga aagcagttta
     1981 aaattgttgc tgaacttctc aaaaaggcaa atacaattat tgtcgcaaca gatagcgaca
     2041 gagaaggtga aaatatcgcc tggtcgatta tccataaagc aaatgccttt tctaaagata
     2101 aaacgtataa aagattatgg atcaatagct tagaaaaaga tgtgatccga agcggttttc
     2161 aaaatttgca accaggaatg aattactatc ccttttatca agaagcgcaa acacgccaaa
     2221 ttgccgattg gttgatcggc atgaacgcaa gccctttata taccttgaat ttacaacaaa
     2281 aaggcgtaca aggtacattt tcattaggac gtgtacaaac accgacttta tatctcattt
     2341 atcaacgcca ggaagcaata gaaaacttta aaaaagaacc ttttttcctg aataattcat
     2401 agttttacta atagtctttc ctaacattga tttaatagtc tttaaagtat tctgcttcat
     2461 cacccattag gtgatgaaaa aagaacccct ttctgattta gcggacaaat actttgacct
     2521 aatctcagaa gatataaaag aaaaaataat aaaatatccg atttatactt tgtataaaga
     2581 actaaagtag ttagcaaatc atttacagca aatcactatg ttccacatac cattttacat
     2641 acttacctta tccacgacac atgttgagat agtatcgaag ataaagaaag ttgctccatg
     2701 ttgtttttgc gagatgggga aatcaaagcc acattgacaa ccatgatgaa taagcttgct
     2761 ttcagtcata agctaatcct agaaccactt ttcaagtcag ttagccaaat cgatgaagaa
     2821 agcgaccgtg aaagaatgga cgctattgat aagctaatgg agcaactatt ggaagaacgc
     2881 aataccctta ttgcactgat gtccaaaggt ttcctagaac ctgctctttt taatcaggaa
     2941 cgaaatgttt tagatagtga gattaaaaat cttactaccg aaaaaacaaa cctggtaacg
     3001 aactctgcga gtggggtttt gcgagcaaac gagataaagg acctcattaa ttatgtgtca
     3061 gcagataatt ttaatggtga ctacacggaa gaactatttg aagaatttgt agtgaacatc
     3121 attgtaaatt ccagggatga gctgacattc aatttgaaat gcggtctttc cctgaaagaa
     3181 aaggtggtga gataaatggc atatattcca tatggataca aaattcaaga tggagtggtt
     3241 actgtcgatg aaaaggcagc aggtcaagta aaggtattct ttgagaaata catatcagga
     3301 ctatccctta cagtggctgg cgaacaggca ggtattgaaa agacacattc tgtgatgggg
     3361 cgcattttga aaaacgtcaa ctaccttgga aatgatacgt atccagcaat tattgataaa
     3421 gagatatttg ataaagctga agaagttaga gataaacgtg caaaggattt aggacgagtg
     3481 gtagagcttg ccgctttcac ctctccccct cccaaagaac gattcaaaat gaaaaaggca
     3541 gataataaga tgccagtgga tccttttgaa cgagcagaat acttatacag tctgatagaa
     3601 agcgaggaat aaagtgacag agaaaaatat aatggttatt cctgctcgta aaagagtagg
     3661 aagtacagcc gcaaaagaaa agataaagaa acttcgtgtt gctgcctatt gccgtgtttc
     3721 tacagaaaca gaagaacaaa attctagcta tgaggttcag gtctcacact atacagagtt
     3781 tataaagaaa aataatgaat gggagtttgc tggcatcttt gcagatgatg gtatctctgg
     3841 tacgaacact aaaaagcgtg acgaatttaa tcgcatgatt gcagagtgta tggacggtaa
     3901 catcgacatg gttattacaa aatccatcag ccgatttgcg cgtaacaccc tagactgcct
     3961 tcaatacatt agacagctca aggataagaa catatcagtt tattttgaga aagagaacat
     4021 caacaccacg gatgccaaag gtgaggtttt gctgactatt atggcatctt tggcacagca
     4081 ggaaagccag agcctttcac aaaacgttaa gcttgggcta cagtaccgat accaacaagg
     4141 aaaggtgcag gtcaaccata agcgatttat gggctacacg aaagatgaag atggaaattt
     4201 aactattgtc cccgaagaag ctgagattat caaacgcatc taccgagaat accttgaggg
     4261 tcagagtcta gtgggcattg gtcgagccct tgaaaaggat ggtattttaa cagcagcggg
     4321 aaaaccaaga tggcgaccag aatcagttaa gaagatactt caaaacgaaa aatacatcgg
     4381 agatgccctt ctgcaaaaga ctgtcacagt agattttctg accaagaaac gagtgaaaaa
     4441 tgaagggcat gttccccaat attatgttga aaatagccat gaagcgatta ttcctaaaga
     4501 gttatttttg caggcgcaag aagaaattca tcggagaagc aatatctaca caggagaagg
     4561 caagaacaaa agaatttaca gtagcaagta cgctttaagt gccatcacct tctgtggaga
     4621 ttgtggcgat atttatagac gggtctattg gaatatccac ggaagaaaag aatttgtctg
     4681 gcgatgcgtg actagaatcg agcaaggtcc tgaagtttgt aagaaccgaa ccgtaaaaga
     4741 agatgaactt tatggtgcag taatgactgc gattaataag ctacttgcag gtgggaacaa
     4801 tatgataaag acactggaag agaatattca tgctgtgatt ggcgaaacca cagaatacca
     4861 aatttcagag attaacaact cactagagga aaaacaaaaa gaactaatca agctggcgaa
     4921 caagggtcaa gactatgacc acttagcaga tgagattgat gagctgagag acaagcgaca
     4981 gatcctttta gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat
     5041 tgaatttatc cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa
     5101 gataatccag agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat
     5161 cgaaatggaa atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg
     5221 ttttcttttg cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat
     5281 ggtttataat attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta
     5341 aagaactgtg tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga
     5401 ctccacagaa tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg
     5461 ccattgctga tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag
     5521 aaataaaaat atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat
     5581 agtaacagag aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg
     5641 tgcaagaggc acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga
     5701 ctcatttgac ctgacagcga ttaatcaaat tgctacagag ttggatgatg agaatagaaa
     5761 caaccttgtt gtacctcccg gagcgtgggg tgattgggtt aatggcggtg gatggttagt
     5821 tataaacggg tgtcatgttg acttgatatt acgtgatata aaacgggtgg aacaaataat
     5881 caaagatacg gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta
     5941 tataagtgct atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga
     6001 aagcttatgc gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt
     6061 gatgaacttt tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg
     6121 ggcagaggat aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca
     6181 agtactattt gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct
     6241 tgaaactttt gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt
     6301 actcggtctt tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa
     6361 tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca
     6421 aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac
     6481 tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc
     6541 gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg
     6601 cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa
     6661 cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg
     6721 agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt
     6781 taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac
     6841 atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac
     6901 tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag
     6961 aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata
     7021 gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg
     7081 ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt
     7141 aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga
     7201 tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc
     7261 acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta catattttgt
     7321 aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat
     7381 aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta
     7441 tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt
     7501 agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat
     7561 taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga
     7621 gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg
     7681 ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct
     7741 gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa
     7801 ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta
     7861 ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag
     7921 ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa
     7981 tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga
     8041 tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca
     8101 gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata
     8161 ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta
     8221 aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt
     8281 gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt
     8341 aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat
     8401 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     8461 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     8521 tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     8581 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     8641 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     8701 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     8761 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     8821 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     8881 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     8941 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     9001 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     9061 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     9121 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     9181 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     9241 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     9301 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     9361 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     9421 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     9481 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     9541 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     9601 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     9661 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     9721 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     9781 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaaat
     9841 aattcctgcc aaagaaattg cacagcgatt taatataagc ttaaggacag tttacagaga
     9901 tatccggaca cttgaagaag ccggaatacc aattggatct gaggccggaa aaggatattt
     9961 tcttgtagag ggcttcctac ttccgccggt aatgtttaca gcggcggaag tgggtgcatt
    10021 gattacagca gggaaatttt taaattgcca tggagatgaa tcatttataa aggattttga
    10081 ttcagctatg tataaaatca aatctatttt gaagcatggt gaaaaaaact atgcacagga
    10141 gttggagaac agtattaacg tgtacagcac atctggacag aaaaatacat tggcggataa
    10201 cgttattgca gcaattcaga ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc
    10261 tgcatcagga gggcaagaac cggaaagcag aatgatagag cctatatcac tggggtttta
    10321 tgaacagaac tggtacttaa tcggttttgc aggctaagaa atgaatatag gaattttcgt
    10381 gtggatcgga ttaaaagcat ttgcatagaa gaagagctct gtcaaagcca tgatggctct
    10441 ttggaacaaa tattaaaaca aatgctttca tataaaaaac tatatgccct acaatgccgt
    10501 tgtaaaaatt ggggaaaagg gatatattga ttttatatct ttctcaaaat aaatataaaa
    10561 gccctttaaa gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga
    10621 ttaatcacaa atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa
    10681 agaatggggc gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat
    10741 tttaaacaat aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg
    10801 aaagtccaaa ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt
    10861 aatgatcgat aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt
    10921 agaattgcca cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat
    10981 tattcatttg actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc
    11041 aaaattaact cgctcatgga cgtttgaaaa tgaaagtaga ttgcttgcta ctcttttagc
    11101 acctttaaaa agtgactatg atcttattat tattgatact gtaccaacgc caagcgttta
    11161 tacaaataat gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga
    11221 aagtacaaac aacattcaaa actatatttc ctatttgatt gatttgcaag aacagtttaa
    11281 ccctggacta gatatgattg gttttgttcc ttatttagtt gatacggaca gcgcaacgat
    11341 aaaatcaaac ctggaagaac tgtacaaaga acataaagag gataatttgg ttttccgaaa
    11401 tattatcaag cgaagtaata aagtaagtac ttggtctaaa aatggcatta cagaacacaa
    11461 aggctatgac aaaaaagttt tgtctatgta tgagaacgta ttttttgaaa tgattgagcg
    11521 aatcattcaa ttagaaaatg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa
    11581 atcacttgtg atttgtgata ggtgatgata aaataaatag taagaagaaa tagaaagaag
    11641 tgagcgatcg tgggaaattt aggcgcacag aaagcaaaac gaaatgatac gccaatcagc
    11701 gcaaaaaaag atataatggg ggataagacg gttcgtgttc gtgccgactt gcaccatatc
    11761 ataaaaatcg aaacagcaaa gaatggcgga aacgtaaaag aagttatgga tcaagcctta
    11821 gaagaatata tacggaaata tttacctgac aaactttaaa aggagtgaaa atgaaatggc
    11881 agttacgtat gaaaaaacat ttgaaattga aatcattaat gagttatcgg caagtgttta
    11941 taatcgggta ttaaattatg ttttgaatca tgaactagat actaaaaata ctcgtttact
    12001 agaagtgaat cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc
    12061 atttgaagaa ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca
    12121 aattttgaat aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc
    12181 aatttgaaga tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg
    12241 aatcgccaac cgctttttta cttggtgggc aaccagggtc agggaaaacc agtttgcgat
    12301 cagcaatttt tgaagaaaca caagggaatg ttattgtcat tgataatgat acctttaaac
    12361 aacagcaccc aaattttgac gaattagtga aactctatga aaaagacgta gtaaaacacg
    12421 ttacccctta ttcgaatcgc atgacagaag cacttattag ccgtttaagc gatcaaggct
    12481 ataatttagt gattgaagga acaggacgaa caacagacgt tcccattcaa acagccacaa
    12541 tgcttcaatc taaaggttat gaaacaaaaa tatacgtcat ggcagtaccg aaaattgaat
    12601 catacttagg aacgattgaa cgatatgaaa ccatgtatgc agatgatcca atgacagcta
    12661 gggcaacacc aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa
    12721 cccttcataa aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac
    12781 tctattcaag cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa
    12841 atcgtaaagt atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg
    12901 ttcaaaatca acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct
    12961 tgcagctacc tacaccacca atacccaaaa cacctaaact tccaggtatt taaaccttaa
    13021 aaaaggaaaa gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc
    13081 tcatgttggg gtcccgagcg cctacgagga atttgtatcg ataagaaata gatttaaaaa
    13141 tttcgctgtt tttttctaat ttcattttga ggtgaattaa ttggtagtta ttagaggtgc
    13201 cctataccta ataatttatc tacattccct ttagtaacgt gtaactttcc aaatttacaa
    13261 aagcgactca tagaattatt tcctcccgtt aaataataga taactattaa aaatagacaa
    13321 tacttgctca taagtaacgg tacttaaatt gtttactttg gcgtgtttca ttgcttgatg
    13381 aaactgattt ttagtaaaca gttgacgata ttctcgattg acccattttg aaacaaagta
    13441 cgtatatagc ttccaatatt tatctggaac atctgtggta tggcgggtaa gttttattaa
    13501 gacactgttt acttttggtt taggatgaaa gcattccgct ggcagcttaa gcaattgctg
    13561 aatcgagact tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca aggtacgctt
    13621 gtagaatcct tcttcaacaa tcagatagat gtcagacgca tggctttcaa aaaccacttt
    13681 tttaataatt tgtgtgctta aatggtaagg aatattccca acaattttat acctctgttt
    13741 gttagggaat tgaaactgta gaatatcttg gtgaattaaa gtgacacgag tgttcagttt
    13801 taatttttct gacgataagt tgaatagatg actgtctaat tcaatagacg ttacctgttt
    13861 acttatttta gccagtttcg tcgttaaatg ccctttacct gttccaattt cgtaaacggt
    13921 atcggtttct tttaaattca attgttttat tatttggttg agtacttttt cactcgttaa
    13981 aaagttttga gaatatttta tatttttgtt catgtaatca ctcctgaagt gattacatct
    14041 ataaataaat acagaagtta aacgatttgt ttgtaatttt agttatctgt ttaaaaagtc
    14101 ataagattag tcactggtag gaattaatct aacgtattta tttatctgcg taatcactgt
    14161 ttttagtctg tttcaaaacg gtagatgttt tatctacatt acgcatttgg aataccaaca
    14221 tgacgaatcc ctccttctta attacaaatt tttagcatct aatttaactt caattcctat
    14281 tatacaaaat tt
//
LOCUS       DACCWV010000072        13731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCWV010000072 DACCWV010000000
VERSION     DACCWV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.072"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            130..1557
                     /locus_tag="IVW84_001777"
     CDS             130..1557
                     /locus_tag="IVW84_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7917970.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1649..2014)
                     /locus_tag="IVW84_001778"
     CDS             complement(1649..2014)
                     /locus_tag="IVW84_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7917971.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2011..2376)
                     /locus_tag="IVW84_001779"
     CDS             complement(2011..2376)
                     /locus_tag="IVW84_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7917972.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2568..2816)
                     /locus_tag="IVW84_001780"
     CDS             complement(2568..2816)
                     /locus_tag="IVW84_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917973.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2815..2943
                     /locus_tag="IVW84_001781"
     CDS             2815..2943
                     /locus_tag="IVW84_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7917974.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3000..4694
                     /locus_tag="IVW84_001782"
     CDS             3000..4694
                     /locus_tag="IVW84_001782"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7917975.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4698..4817
                     /locus_tag="IVW84_001783"
                     /pseudo
     CDS             4698..4817
                     /locus_tag="IVW84_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4899..5090
                     /locus_tag="IVW84_001784"
     CDS             4899..5090
                     /locus_tag="IVW84_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917976.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            5468..6601
                     /locus_tag="IVW84_001785"
     CDS             5468..6601
                     /locus_tag="IVW84_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7917977.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6632..6952
                     /locus_tag="IVW84_001786"
     CDS             6632..6952
                     /locus_tag="IVW84_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7917978.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6981..7445)
                     /locus_tag="IVW84_001787"
     CDS             complement(6981..7445)
                     /locus_tag="IVW84_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7917979.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7576..8829)
                     /locus_tag="IVW84_001788"
     CDS             complement(7576..8829)
                     /locus_tag="IVW84_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7917980.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8858..9175)
                     /locus_tag="IVW84_001789"
     CDS             complement(8858..9175)
                     /locus_tag="IVW84_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7917981.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9201..9521)
                     /locus_tag="IVW84_001790"
     CDS             complement(9201..9521)
                     /locus_tag="IVW84_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7917982.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9985..11400)
                     /locus_tag="IVW84_001791"
     CDS             complement(9985..11400)
                     /locus_tag="IVW84_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7917983.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11417..12733)
                     /locus_tag="IVW84_001792"
     CDS             complement(11417..12733)
                     /locus_tag="IVW84_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7917984.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12883..13581)
                     /locus_tag="IVW84_001793"
     CDS             complement(12883..13581)
                     /locus_tag="IVW84_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7917985.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4364 a         2787 c         2359 g         4221 t
ORIGIN      
        1 ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg
       61 atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga
      121 ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt
      181 gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt
      241 tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc
      301 gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga
      361 tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat
      421 ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc
      481 aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc
      541 gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg
      601 tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat
      661 cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa
      721 atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata
      781 gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca
      841 tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca
      901 ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa
      961 cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt
     1021 gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat
     1081 ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta
     1141 aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc
     1201 cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg
     1261 agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat
     1321 gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt
     1381 gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac
     1441 cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt
     1501 cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg
     1561 gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa
     1621 tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct
     1681 ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa
     1741 tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca
     1801 aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt
     1861 cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa
     1921 caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat
     1981 atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc
     2041 tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag
     2101 tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac
     2161 gactagtact tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt
     2221 acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag
     2281 aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat
     2341 ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat
     2401 ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc
     2461 taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc
     2521 ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc
     2581 agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa
     2641 ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca
     2701 gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct
     2761 aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt
     2821 aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta
     2881 ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc
     2941 tagcatccta tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa
     3001 tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt
     3061 atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc
     3121 acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc
     3181 atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta
     3241 cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga
     3301 ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt
     3361 tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc
     3421 gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata
     3481 tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta
     3541 tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata
     3601 tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac
     3661 gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat
     3721 gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag
     3781 atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg
     3841 ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta
     3901 ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc
     3961 tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg
     4021 atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga
     4081 atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa
     4141 ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctatcgtc gtgcatcatc
     4201 aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc
     4261 caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc
     4321 tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa
     4381 aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt
     4441 ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga
     4501 ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac
     4561 aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc
     4621 gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa
     4681 aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
     4741 ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag
     4801 cttttgaaca ccgtttacca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     4861 cacccaagct aaaactgctt cttatgtttt tgacttggtt ggacttatta ccgcagatgc
     4921 aagcttttga acaccattta ccaagaggtt atgaaaaagc tgccctgcat caagtaataa
     4981 gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta ttaccgcaga
     5041 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag tttcgagtat
     5101 gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga
     5161 gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa
     5221 ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat acaactattt
     5281 caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca aatacataag
     5341 actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct
     5401 ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc
     5461 atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga
     5521 tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg
     5581 cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt
     5641 aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa
     5701 agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa
     5761 tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg
     5821 atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta
     5881 tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt
     5941 tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg
     6001 cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt
     6061 tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc
     6121 ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga
     6181 attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg
     6241 attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc
     6301 tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt
     6361 ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt
     6421 caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa tgattgcaga
     6481 aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca
     6541 agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg
     6601 aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga
     6661 gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gcttctattt
     6721 ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga ttttctatat
     6781 ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct atttttattt
     6841 ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga acatttttat
     6901 ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat gagaattttt
     6961 caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg agcggtcgtc
     7021 ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata aaacgtggta
     7081 tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt tctgatttcc
     7141 gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa cagggcacaa
     7201 agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc cgctcctccg
     7261 acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc cttattagcg
     7321 attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt tgatgaaaac
     7381 caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca gttatatgta
     7441 accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca agtctttatg
     7501 aaaattagca atgacaacaa tagtgatgta aaaaaaaaac ggaaaacaat cacttgttct
     7561 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7621 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7681 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7741 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7801 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7861 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7921 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7981 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     8041 gctatgccca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     8101 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     8161 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8221 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8281 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8341 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8401 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8461 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8521 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8581 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8641 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8701 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8761 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8821 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8881 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8941 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     9001 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     9061 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     9121 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9181 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9241 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9301 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9361 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9421 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9481 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9541 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9601 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9661 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9721 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9781 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9841 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9901 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9961 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
    10021 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
    10081 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
    10141 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10201 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10261 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10321 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10381 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10441 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10501 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10561 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10621 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10681 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10741 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10801 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10861 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10921 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10981 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    11041 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    11101 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    11161 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11221 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11281 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11341 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11401 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11461 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11521 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11581 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11641 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11701 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11761 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11821 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11881 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11941 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    12001 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    12061 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    12121 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12181 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12241 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12301 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12361 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12421 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12481 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12541 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12601 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12661 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12721 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12781 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12841 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12901 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12961 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    13021 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    13081 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    13141 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13201 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13261 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13321 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13381 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13441 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13501 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13561 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13621 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13681 catagacata caaacgaaga agatatttac cataaaaaag actaggataa a
//
LOCUS       DACCWV010000073        13650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCWV010000073 DACCWV010000000
VERSION     DACCWV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.073"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            26..268
                     /gene="yaaA"
                     /locus_tag="IVW84_001794"
     CDS             26..268
                     /gene="yaaA"
                     /locus_tag="IVW84_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7917986.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            255..1379
                     /gene="recF"
                     /locus_tag="IVW84_001795"
     CDS             255..1379
                     /gene="recF"
                     /locus_tag="IVW84_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7917987.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1376..3322
                     /gene="gyrB"
                     /locus_tag="IVW84_001796"
     CDS             1376..3322
                     /gene="gyrB"
                     /locus_tag="IVW84_001796"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7917988.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3342..5813
                     /gene="gyrA"
                     /locus_tag="IVW84_001797"
     CDS             3342..5813
                     /gene="gyrA"
                     /locus_tag="IVW84_001797"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7917989.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6049..6348
                     /locus_tag="IVW84_001798"
     CDS             6049..6348
                     /locus_tag="IVW84_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7917990.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6398..6910
                     /gene="ssb"
                     /locus_tag="IVW84_001799"
     CDS             6398..6910
                     /gene="ssb"
                     /locus_tag="IVW84_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7917991.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6936..7172
                     /gene="rpsR"
                     /locus_tag="IVW84_001800"
     CDS             6936..7172
                     /gene="rpsR"
                     /locus_tag="IVW84_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7917992.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7315..9288
                     /locus_tag="IVW84_001801"
     CDS             7315..9288
                     /locus_tag="IVW84_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7917993.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9294..9746
                     /gene="rplI"
                     /locus_tag="IVW84_001802"
     CDS             9294..9746
                     /gene="rplI"
                     /locus_tag="IVW84_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7917994.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10020..11387
                     /gene="dnaB"
                     /locus_tag="IVW84_001803"
     CDS             10020..11387
                     /gene="dnaB"
                     /locus_tag="IVW84_001803"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7917995.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12167..13459
                     /locus_tag="IVW84_001804"
     CDS             12167..13459
                     /locus_tag="IVW84_001804"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7917996.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4543 a         2470 c         3009 g         3628 t
ORIGIN      
        1 taataatgaa aatgagggat gggcattgaa acaacaagtc attctaaata cggaattcat
       61 gacgttgggc caaatgttga aagaagtcac agttatcggt agtggcggac aagcaaaatg
      121 gtatttggca gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc gtggacgcaa
      181 actttatccc ggcatgatgg ttgaagttcc agaagtagga acttttttta tggtaaaaaa
      241 aggaaacgac gataatgagg ctgaataagc tgtatttaaa aaattatcga aattacgaag
      301 agctgaacat cgaattttcc aagaacctcg tcatcttttt aggagaaaat gcgcaaggaa
      361 aaacgaatct tttggaaagt atctacgtat tagctatgac gcgaagtcat cggacaagta
      421 gtgagcagga attgattggc tggactgacg atcaggcgat gattcaagga gagatcacaa
      481 aaggttcatc cactcttcca ttagaaatct tattatctaa aaaaggccga aaaaccaaga
      541 tcaaccatat cgaacaaaaa aagttaagta cttacgtggg acaaatgaat gtcattttat
      601 ttgcaccaga agatctttct ttagtaaaag gcagcccgca gatcaggcgg aagtttttag
      661 atatggaatt aggccagatc aatccaatct atctttatga tcttgtccaa taccaagcaa
      721 ttttgaaaca aagaaaccag tatttaaagc aattgaatga aaaaaaacaa actgatttgc
      781 tttatttgga tattttgtcc gaacaactag cagcgtttgg cagcaaagta ttgaaagcaa
      841 gagcacagtt cgtcaagcga ttggagtttt gggctaattc tttgcatcag caaatcaccc
      901 atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt cgattcttta gaaacacata
      961 cgcaagagca aatccaagaa caatttctcg cacttttgaa ccaaaataaa aagaaagacc
     1021 tttttcgggg aacaacgaca gttggtcctc atcgtgacga tctttccttt tttattaatc
     1081 agaaaaatgt gcagacttat ggatcgcaag gacagcaaag aacaactgca ctaagtgtca
     1141 aattagcaga aatcgatttg attaaagaag agacggggga atatccaatc cttttgttag
     1201 atgatgtgat gagcgagctg gatgatagtc gtcaactcca tctgctagaa acaatcgaag
     1261 gaaaagttca aacgttctta acaacaacga cacttgacca tgtaaaaaat aaaatgactg
     1321 ttgaacctga gattttttat gttcaacaag ggaaactaaa aggagaagaa actgaatgac
     1381 agaagaaaga agtttagtag aacgcgctaa agagtatgat gccagtcaga ttcaagtact
     1441 tgaaggactt gaagccgttc gtaaacgacc tggcatgtat atcggttcga ctagttctga
     1501 gggtctgcat caccttgttt gggaaattgt cgataattcg atcgatgaag tattagcagg
     1561 ttttgcaacg aaaatccatg tgattattga aaaagacaac agtatcaccg ttattgacga
     1621 cggtcggggg atacctgtag atatccaggc aaaaactgga cgtcctgcag ttgaaacggt
     1681 ctttactgtt ttacatgctg gaggaaaatt cggcggtggt gggtacaaag tttctggtgg
     1741 acttcacgga gtaggatctt ctgttgttaa cgcgctttct acacagcttg atgtcaaagt
     1801 atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag tggacgactt
     1861 aaagattatc gaacaaacgg atcgccatgg aacaacggtt cactttacgc cagaccctga
     1921 aatttttaca gagacaactg agtttgattt ttcaaaattg gctacccgga tccgtgaact
     1981 tgctttcttg aaccgcggga tgcgtatatc gattgaagac aaacgagaag aagaaccagt
     2041 catcaatgaa taccattatg acggcgggat taaaagttat gtcgaatact tgaatgcaaa
     2101 caaaaccgtg atttttccag aacctgtata tttggaaggt gaacagcaag atatcgcagt
     2161 tgaagtctct atgcaatata cagacggcta ccattccaat atcatgagtt ttgccaacaa
     2221 tatccatacg tatgaaggcg gaacacatga atcaggattc aagactgctt taactcgtgt
     2281 gataaatgat tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca acttgaccgg
     2341 tgaagacgtg cgagaaggat tgacagctgt tatttctatc aagcatcctg accctcaatt
     2401 cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta cggacagtca ctgatcggtt
     2461 atttagcgaa catttcatga aattcttgct ggaaaaccca agtgtgggcc gtcaaattgt
     2521 tgaaaaagga ttactagctt ccagagctcg tctagcagcg aaacgtgcgc gtgaagtaac
     2581 gagacgtaaa ggtgctttag agattagtaa tctgccagga aaattagcgg actgctctag
     2641 caacgatcct gaaaaatgcg aattgttcat cgtcgaagga gattcggccg gcggatcagc
     2701 taagcaaggt cgtaatcgag agtttcaagc catcttgcct attcgtggga aaatcttgaa
     2761 tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa gagatccggt ctttatttac
     2821 tgctatgggt acagggtttg gagaagattt tgatgtatcg aaagctcgct accacaaatt
     2881 agttatcatg actgatgccg atgtcgacgg cgcgcatatc cgtacattgc tgctgactct
     2941 tttttatcgt tacatgcgtc ctattgttga agctggatac gtctatattg ctcagccacc
     3001 tttatacggg gtaaaacaag ggaagaatat tacgtatgta caaccaggaa aaaatgcaga
     3061 agaagagttg aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa gcgttcagcg
     3121 ttataaagga cttggagaaa tggatgatca tcaactatgg gaaacaacta tggacccaag
     3181 caatcggatg atggcccgcg tcagtgtgga cgatgctatt gcagcagatc agatatttga
     3241 aatgttgatg ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa atgcacacta
     3301 tgtgaagaac ttagatattt aatcatgaag gaggaaaaag aatgagtgaa gaaatcagag
     3361 aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc gattatgcga
     3421 tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa cccgttcatc
     3481 gccgtatttt atacggcatg aacgaattag gtgtaacacc ggataaagcc cataaaaaat
     3541 ctgccagaat cgtcggagat gtcatgggta aataccatcc tcatggagac tatgcgatct
     3601 atgagtcaat ggtacggatg gcacagccat tcagctaccg ttatatgcta gtggatggtc
     3661 atggaaactt tggttctgtt gacggtgacg gtgccgccgc tatgcgttat accgaagcac
     3721 ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa taagaacact gttgatttcc
     3781 aaagcaacta tgatgataca gaaaaagaac cagttgtact acctgctcgc ttcccgaatc
     3841 ttttagtgaa cggaacaacg gggatcgccg taggtatggc cacgaatatc ccacctcata
     3901 acttgtctga ggtagtagcg gctatcgatt tgctgatgga taatcctgat gtgacgacca
     3961 atgaattgat ggaagtatta cctggaccag attttccaac aggtggtttg gttatgggaa
     4021 aatcaggaat ccgaagagcg tatgaaacag gtaagggttc tattaccgta cgtgcaaaag
     4081 ttgagattac tgagatgcca aatggtaaag aacggatcat cgtgacagaa ttgccttata
     4141 tggtcaataa agcaaaattg atcgaacgga tttctgaact gcacagagac aagcgtatcg
     4201 aagggatcac agatttgcgg gacgaatctt cccgtgaagg tatgcggatc gtcattgaca
     4261 tccgtcgtga cgccagcgca tctgttatct tgaataactt atataaattg acatccttgc
     4321 aaaattcatt tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa gttttgagct
     4381 tgaaacaaat tcttgaaaac tatatcgaac atcagcgcga agtcatcaca agacggacag
     4441 tctttgaaaa agaaaaagca gaagcaaggg cacacatttt agaaggtttg cgtattgctt
     4501 tggatcatat cgatgaaatc attgcgatta tccgcgggtc taaatcagat gacgaagcaa
     4561 aagcgacgat gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct attttagata
     4621 tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa taccaagaac
     4681 tcttaaaact gattgaagat ttaactgaca ttctggcaag accagaacgt gttacagaaa
     4741 tcatcaagaa tgaattagca gacttgaatc aacgatttgg tgataaacga cgcacagaat
     4801 tactagtcgg tgaggtctta agtctggaag atgaagattt aatcgaagaa gaagaaatcg
     4861 ttattacatt gacaaacaat ggttacatca aacgtttggc aaataatgaa ttcagagctc
     4921 aacgaagggg tggacgtggt gtccaaggaa tgggtatcca caatgacgac ttcgtcaaaa
     4981 atctggtttc ttgttctacc catgatacat tgttattctt tacgaataac ggaaaagtgt
     5041 atcgggcaaa aggatacgaa attccagaat acgggcgcac agccaaagga ataccaatta
     5101 tcaatttact aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg gtgacgggag
     5161 aagcagaaga aggccactat ctcttcttta cgacgcgtca aggtacagta aaacgtactt
     5221 ctgtcactgc ttttgctaac atccgaagta atggtctgat cgcaattggt ttgaaagaag
     5281 acgatgagct cgtcaatgtg ctactgacag atggtcaatc taatatcatt attggaacac
     5341 ataacggcta ctctgtcact ttcaaagaag aagcagtccg cgatatggga cgtacagctt
     5401 ccggggttag aggaatacgc ctgcgtgagg aagattatgt cgttggggca gccttaatga
     5461 aggaagatca agaagtatta gtgatcacag aaaaaggtta cggaaaacgg acaaaagtat
     5521 cggaatatcc agtgaaaggc cgcggaggta aagggatcaa aacggcgaac atcactgaaa
     5581 aaaatggtcc actagctgga ttgaccactg tttctggaga tgaagacatc atgctgatta
     5641 cagataaagg tgtcattatc cgtttcaacg tatcgactgt ttctcaaact ggacgttcaa
     5701 cattaggcgt acgtttgatg aagatggaag ctgatacgaa agttgtcacg atggcagcag
     5761 tcgaaccaga agctgcagaa caaacagttg aagaacaagg atcagaagaa tagccatcaa
     5821 gactaattgt gtaagctagc aagaatgacc gattaaaaaa tctggttatt cctgctggct
     5881 tttttgatta gttgaatttc tcttaaaata ttcgaaactg ttcattctgt attgcttttg
     5941 accaaaatag agtgtataat aagagcttgt gagtagtcta attaattaga taactctcct
     6001 tgctcttagg aacctaagag ccgaaagtcc acagggaggt gaatatcaat ggaaaacacg
     6061 aaatatgaaa tcatgtacat tattcgtcca aacattgatg aagaagcaaa aactgcttta
     6121 atcgaacgtt tcgatgcaat cttgaaagat aacggagcag aagttatcga atcaaaagat
     6181 tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat ctatcacatc
     6241 gttaaagtat cttctccatc atcagcaaat gctgtcaatg aatttgaccg tcttgctaaa
     6301 atcaacgacg atatcattcg tcacatgatt gttaaagaag aagcttaatt gttgtttcat
     6361 gtgaaacaat agaaaacgat gagaggagat gactattttg attaataatg ttgtattagt
     6421 tggacgtctg acgaaagatc cagatttgcg ttacacagca agtggtacgg cagtcgcaac
     6481 atttactttg gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg aggctgattt
     6541 tatcaactgt gtgatttggc gcaaatcagc agaaacttta gcaaactatg ctcgtaaagg
     6601 aacactttta ggtgttactg gacgtatcca aactcgttct tatgataatc agcaaggaca
     6661 acgtgtatac gtgacagaag tagtagctga caacttccaa ttgctggaaa gtcgttctgc
     6721 atctgaaaat cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag ctggcggatt
     6781 tggcggaaac aataactcta atcaaacatc tcaatcatcc aacggtatgc ctgatttcga
     6841 ccgtgatact tctgacccat tcggctcaag ttcaacgatc gacatttcag acgatgattt
     6901 accattctaa acgaatttga taggagggaa atagaatggc acaacaaaga agaggcggac
     6961 gtaaacgtcg taaagtagac tacattgctg ctaaccatat tgaatatatc gattataaag
     7021 atatcgaatt actaaaaaga tttatttcag aacgcggtaa aatcttgcca cgtcgtgtaa
     7081 ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc gctcgtatca
     7141 tgggcttgct acctttcgtt agcgaagact aataaatcct aggaattgaa tgactgatcc
     7201 ttttgatcag ctattcaatt cctttttttc tgtaaaaata acctctattc gtttagtcgc
     7261 tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat atgggaggaa aattatggaa
     7321 aataaaaaca atcatttatc cccgaaacct ctcttcctta tcctgtttat attaatcgaa
     7381 gtattggtag tcttaatttt accatttcgt tttgttagcg cttcggtcat tctattgatg
     7441 aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt aaataaacgt
     7501 gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc tagcttatgt ctcagaagtc
     7561 atgcctgtag gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg gctcaatcca
     7621 ttagcattga aaatcaaaga tcaaagtgga ttaacagagg aagaattgct agatactttt
     7681 tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac atttcagttt
     7741 gatatggata cagtaaaggg tgttgtttat tttaatgata ttacccaaaa ccagttgtta
     7801 aaactccatc agacgaacag tcagccggtg attggtattt tatctattga taactatgca
     7861 gatgccattg ataaattgga tgacaaagag atttcttatt tgaactcttt cgtcactaca
     7921 ctagtttctg actggatgaa tgaataccat gtctttttca aaagaatcaa cgcagaacgg
     7981 tttttcttcg tagcacagat gatcgatctg aaaaagatga tggataaaaa atttgatttg
     8041 ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct tagtatgggg
     8101 attgcttatg gctcaagaac cctcgatgaa atcggtggag aagctcagaa taatttagat
     8161 gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga aggagcaaaa
     8221 cctgtttatt atggggggaa atctgctaag aatgtcaaac gcacgcgtgt tcgttctcga
     8281 gcgatgagta ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt tatcatgggg
     8341 cacaagtttc cggatatgga tgctttaggc tctgcttttg gtgtcagttg tctggcacgc
     8401 ttcaaccaga aaaaagctta catcgttatc aacgagaacg agatcattcc agatgtagaa
     8461 cggtgtcttg aagaaatcca taagcatcct gaattagaaa tgcaactaat ctctccagaa
     8521 cgtgccatag agctgaaaaa agaagaggac ctcctcgtga tggtggatta ccataaacct
     8581 tctctatcga tctctcaggg cctatatgag caatttgata agattgtgat cattgatcac
     8641 catcgtcgtg gggaagagtt tccaagtaaa cctctgctga cttatatcga atcttcagct
     8701 tcttcagctt cagagctagt ttctgagctg atccaatacc aaagcagcaa aacaaatcgc
     8761 ttagatcgtt ttgaggccac attattgctt gcagggatgg ttgtagatac aaatagtttc
     8821 aatgtccgta caactgcacg aacttttgac gttgccagct atttaaggtc ttgtggagca
     8881 gacgcatcct tagtacaata tttattgagc tcggatttag cgacatactt ggagatcagc
     8941 aatttgattg ctggaagcga ctatgtgact tctgatattg ttgtggcggc agggacagac
     9001 gaaaaagagt ataatagtgt tatagcagca aaaacagcag atacattgct ttcgatgatc
     9061 aatatcaatg cagctttcgt cattacaaag cggacagacg gcttgattgg gatcagtgcc
     9121 agaagtaccg gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg cggaggacac
     9181 ttcacaaacg cagctactca actggaaaat acgacagttg aggaaacaaa acagaagcta
     9241 tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata aggagtaatc caaatgaaag
     9301 taattttttt acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa gaagttccag
     9361 caggttatgc acaaaattat ctaatcaaaa ataacttggc aaaagaagca acgaaaagca
     9421 gtatcagtca gttagcagga caaaagaaag cacaagaaaa acacgaagct gaaatcttgg
     9481 aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc gaattgaagg
     9541 cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa attgctgaag
     9601 ggttaaacaa acaatacaaa atcaaattag ataaacgaaa aatcgaatta gaaaatccga
     9661 tccgttcact aggctacaca aacgtacctg ttaagcttca tcatgaggtg acggctacga
     9721 tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc tggtaagtat
     9781 aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa aacagctgca gcaatgaaag
     9841 caaaagctgc aaatcgattt cagattgatt tcggtttctt ttttgggaac agcaaaatag
     9901 cataaagaaa acgagtataa taaaagagaa ctattttttt attacacttt cggtacgaat
     9961 gatgactttt ctctcatttt cttgtaaaat gaagcaattg aagacaagga gcgatacgta
    10021 tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa caagcggttt
    10081 taggctctgt ttttcttgac gcagaaacga tcattgatgc catggagtac atagagccaa
    10141 aagactttta tcgtcgtggc catcaaatta tttttgagac aatgatcgag ttaaatgatc
    10201 gtaatgaagc gattgacgtg gttactgtca aagaccggct ggaacaagcc aatttgttag
    10261 aagatgccgg tggtttaagt tatctctcag acttggcatt agcagttccg acagcagcaa
    10321 atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca ttgatccaga
    10381 cagcaacagg aatcgttacg aaaggattcg agcaaggaga agaagttcaa acaattttag
    10441 atgatgccga acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc ggctttttat
    10501 ctattgctga agtactgaac tccacgattg aaaatatcga tcgactagct caaaacaatg
    10561 aagaaatcac aggtctacca actggctatc aaggtctgga taaaatgaca gcaggtttcc
    10621 agcccgaaga actaatcatt cttgctgcac gtccagcggt aggtaaaaca gcgtttgcat
    10681 tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata tttagtttgg
    10741 aaatgggggc agaatctctt gtaaatcgga tgctttgtgc agaaggttcg attgaggcca
    10801 gccatttacg tacaggtcag ctttcggaag aagaatggtc gaatctgatc gttgcgatgg
    10861 ggagtctttc tcgagcaaat atttttatcg atgatacacc agggatcaaa atctctgaaa
    10921 tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg atattgatcg
    10981 attatttgca gttgattgaa ggtaacggta aagagaaccg tcaacaagaa gtatcagata
    11041 tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt gctctttcac
    11101 agttatctcg tggagtagaa cagcgacaag acaaacgacc agtgctaagt gatattcgtg
    11161 aatccggatc aattgaacaa gatgcggaca tcgtagcctt cttgtatcga gatgattatt
    11221 atgaaagaga aggcggagat gacgatgatg gaaacgagcc aaaaaatgac aacgtcatcg
    11281 aagtcatcat cgagaagaac cgaagcggtg cacgaggaac agttgaacta ctgtttatca
    11341 aagaatacaa taagttttcg tcgctttcgc caagagaaga attttaaaga agaggttgtg
    11401 agccttgagc aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg caggaacgac
    11461 tagagtactt aatttaaaca gatctccaaa gggattgtga caaaagtctt gtcacagtcc
    11521 ctttttccga ataaacggtg ttcaatcagc tgacccttgg tattagctca gcaaacgtca
    11581 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatctt
    11641 acgacctctt ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    11701 catcaaacat gagaagcagt tttttgaaat tgctccttgt gactcagagt taaacggcag
    11761 gctcacaacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa tacgcccaat
    11821 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgcaggaca
    11881 gctttttcac aacctctgat tcacggtgtt ccaaaagtag cttcttcgaa aaataagtcg
    11941 aaatttcaca aaaatttgag aagcaatttt cgtacattcc ttcttatttc ttgaagctga
    12001 ccacttctgt tacaacctct tttttaataa ccattctaaa aagataaagt tcgtttttta
    12061 agcgttttta taaaataaaa ataaaaatca ttcgatttta tttgaaatat gtatctatta
    12121 ttgatacaat ggttcaggat taaataatat agaacgaggt gttcacatgt catctgtagt
    12181 agtagtaggt acgcaatggg gcgatgaagg gaaaggaaaa atcacagact ttctcagtga
    12241 aaatgctgaa gtgattgcac ggtaccaagg tggcgacaat gcagggcaca caattaagtt
    12301 taacggtgtg acttataaac tgcatttgat tccttctggg attttttata aagacaagat
    12361 cagtgtgatc ggaaacggcg ttgtagtcaa tcctaaatca ttggttaaag aattagctta
    12421 tttacatgaa aaaggtgtag aaacaagtaa tttgcgtatt tctgaccgcg cgcacgtaat
    12481 cttgccttat catatcaaat tggaccaatt acaagaagat gctaagggcg aaaataagat
    12541 tggtacaacg attaaaggaa tcggacctgc ctatatggat aaagcagctc gtgtaggtat
    12601 ccgtattgca gacttattag ataaagaaat ttttgaagaa cgtcttcgtt tgaatctaga
    12661 tgacaaaaac cgtcaatttg taaaaatgtt tgattcagaa ccacttgatt ttgacgatat
    12721 ttttgaagaa tattatgaat acggacaaca aatcaaacaa tacgtcacag atacttccgt
    12781 tatcttgaat gatgctttgg atgcaggcaa acgcgtgttg tttgaaggcg cacaaggtgt
    12841 aatgctggat atcgaccagg gaacttatcc ttttgttact tcttctaacc cagtagccgg
    12901 tggggtaaca atcggaagcg gtgtagggcc ttcaaaaatc aataaagttg tcggcgtctg
    12961 caaagcctat acgtcacgtg tcggtgatgg accatttcca actgaattat ttgatgaaac
    13021 aggagaacaa atccgcgaag taggacgaga atacggtacg acaactggtc gtccgcgtcg
    13081 tgtaggctgg tttgattctg tagttatgcg ccattctaaa cgagtttcag gtattaccaa
    13141 cctatcttta aactcgatcg atgtattaag tggattggaa acagtaaaaa tttgtacggc
    13201 ttatgaatta gatggcgaac taatctacca ctatccagca agcttgaaag agttgaaccg
    13261 ctgcaaacca atctatgaag aattgccagg ctggtcagaa gacattacag gatgcaaaac
    13321 acttgcagaa ctgccagaaa acgcacgtaa ctatgtacgt cgtatctcag agcttgttgg
    13381 tgtacgtatc tcaacatttt ctgtcggacc tgatagaaca caaacaaaca tcttagaaag
    13441 cgtctgggcg caaatctaag gataaaaacg acgactgaat caggacgttc ttaccagagc
    13501 gagtcgaagt gtttatctat acgataataa taacttgaag aggtagtgaa agagacgttt
    13561 agccttattg ccgataggct gtctcttgaa cactgtttat tcagaataag ggactgtgac
    13621 aaaaaccgtg tcacagtccc tttttccgaa
//
LOCUS       DACCWV010000074        13523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCWV010000074 DACCWV010000000
VERSION     DACCWV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.074"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(6..2387)
                     /locus_tag="IVW84_001805"
     CDS             complement(6..2387)
                     /locus_tag="IVW84_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7917997.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2377..3024)
                     /gene="recU"
                     /locus_tag="IVW84_001806"
     CDS             complement(2377..3024)
                     /gene="recU"
                     /locus_tag="IVW84_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7917998.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3088..3627
                     /locus_tag="IVW84_001807"
     CDS             3088..3627
                     /locus_tag="IVW84_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7917999.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3717..4136
                     /gene="gpsB"
                     /locus_tag="IVW84_001808"
     CDS             3717..4136
                     /gene="gpsB"
                     /locus_tag="IVW84_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7918000.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4158..4525
                     /gene="rnpB"
                     /locus_tag="IVW84_001809"
     ncRNA           4158..4525
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVW84_001809"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4734..5900
                     /locus_tag="IVW84_001810"
     CDS             4734..5900
                     /locus_tag="IVW84_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7918001.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5946..7442
                     /locus_tag="IVW84_001811"
     CDS             5946..7442
                     /locus_tag="IVW84_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7918002.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7523..8206
                     /locus_tag="IVW84_001812"
     CDS             7523..8206
                     /locus_tag="IVW84_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7918003.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8851..10041
                     /locus_tag="IVW84_001813"
     CDS             8851..10041
                     /locus_tag="IVW84_001813"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7918004.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10143..11096
                     /locus_tag="IVW84_001814"
     CDS             10143..11096
                     /locus_tag="IVW84_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7918005.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11165..12514
                     /locus_tag="IVW84_001815"
     CDS             11165..12514
                     /locus_tag="IVW84_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7918006.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12697..13020)
                     /locus_tag="IVW84_001816"
     CDS             complement(12697..13020)
                     /locus_tag="IVW84_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918007.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13149..>13523
                     /locus_tag="IVW84_001817"
     CDS             13149..>13523
                     /locus_tag="IVW84_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918008.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFG"
BASE COUNT         4272 a         2408 c         2897 g         3946 t
ORIGIN      
        1 ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca
       61 tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc
      121 gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct
      181 gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag
      241 ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga
      301 atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt
      361 tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct
      421 gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg
      481 acaggtgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag
      541 cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca
      601 tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc
      661 gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct
      721 ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg
      781 attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct
      841 gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta
      901 gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa
      961 gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag
     1021 taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga
     1081 gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc
     1141 aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg
     1201 gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca
     1261 atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg
     1321 tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg
     1381 tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt
     1441 tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg
     1501 gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg atcatattct
     1561 gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc
     1621 gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc
     1681 aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct
     1741 gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc
     1801 tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt
     1861 ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc
     1921 aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc
     1981 cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca
     2041 tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa
     2101 tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt
     2161 ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa
     2221 aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta
     2281 tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga
     2341 gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc
     2401 ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga
     2461 aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg
     2521 tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca
     2581 gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc
     2641 tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt
     2701 ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt
     2761 tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac
     2821 tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata
     2881 atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt
     2941 gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg
     3001 gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa
     3061 agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg
     3121 tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa
     3181 aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc
     3241 agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa
     3301 tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat
     3361 gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt
     3421 agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag
     3481 catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt
     3541 tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg
     3601 gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc
     3661 tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg
     3721 cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg
     3781 gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaaactt
     3841 acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc
     3901 aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga
     3961 gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat
     4021 ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata
     4081 actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt
     4141 tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag
     4201 tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa
     4261 ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc
     4321 ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc
     4381 cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac
     4441 aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc
     4501 aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa
     4561 gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga
     4621 atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt
     4681 gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa
     4741 aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta
     4801 aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag
     4861 gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa
     4921 tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat
     4981 tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca
     5041 agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac
     5101 gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt
     5161 ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac
     5221 caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca
     5281 tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg
     5341 tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag
     5401 gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa
     5461 atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt
     5521 cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag
     5581 gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg
     5641 gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga
     5701 aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct
     5761 tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat
     5821 tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa
     5881 aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat
     5941 agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag
     6001 ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc
     6061 gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa
     6121 tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg
     6181 ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta
     6241 tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa
     6301 cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt
     6361 tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg
     6421 aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc
     6481 tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc
     6541 ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt
     6601 atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc
     6661 cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg
     6721 gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt
     6781 atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg
     6841 agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat
     6901 gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca ttgaaccata
     6961 caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca
     7021 ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg
     7081 aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag
     7141 gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat
     7201 tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa
     7261 atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc
     7321 aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta
     7381 atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat
     7441 aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt
     7501 acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga
     7561 ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt
     7621 agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc
     7681 ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca
     7741 gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc
     7801 cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga
     7861 aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg
     7921 tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca
     7981 aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga
     8041 aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg
     8101 aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga
     8161 tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca
     8221 gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt ctggaacact
     8281 gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa
     8341 aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac
     8401 cgtttattcg gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc
     8461 aacaagtaaa acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa
     8521 gatggaaatt ttatgtattt ttattggtct atgcaataaa taaattaaat attcagaata
     8581 caattgattt taacgaaagg gcttctatca atggttgtgt tgtatttatc tgcccaggta
     8641 gagtaaaata gatcattatc taaaaggtaa ttgttcatac tatataagtc atttgaaagt
     8701 aaaaagatac tgtaaataaa aaatgtggtt aaataattct cataatcagt gtaagcgtta
     8761 tcaaaaatat agcatacttg ctttgtagat gaatgacacg atatatgttt ggtatgttct
     8821 gcaagacaga tgaatatgga ggtggagaca atgtcttcca aagaactaac aatgtattta
     8881 gagaaagaat tggatcaact gaaagaaaag gggttataca atacgatcga catactagaa
     8941 agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct agcatccaac
     9001 aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga agcaaccgaa
     9061 acttatggtg taggagcagg agcagtccga acgatcaatg gttcgctgaa aatccatcag
     9121 caattagaag aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc ctttcaatct
     9181 ggattcaact gcaatatggg cgctatctct gcagtaatga caaaagaaga tgctatctta
     9241 tcggatgaat taaaccatgc atctattatt gacggttgcc gcttatccgg agcaaaaatc
     9301 atccgaatta aacatcaaga tatgaaagac cttgagaaaa aagcaaagga agcgatagaa
     9361 agtaagaaat ataaaaaaat catgtatatc acagatggtg ttttctccat ggatggagac
     9421 attgcacgat tgccggaaat tattccgatt gttgaaaaat atggactgat cacctacgtc
     9481 gatgatgcgc acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa gcattttgga
     9541 ttaagcgaca aaatcgatat gcaaatgggc acgttatcga aagctatagg tgttgttggg
     9601 ggttatgtag ctggatcgaa aacactaatc gactggttga aagctagaag tcggccattc
     9661 ctattttcta cttcgctaac tccaggtgca gctgcagctg cattggcttc gattactttg
     9721 atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa atgctaatta ttttaaagaa
     9781 gaattgaaaa aagtaggcta taacatcggt atgtctgaaa cacctattac tccagtcatt
     9841 ttgggagacg aaaaagtgac tcagactttc tctaagaagc tgatcgaaca tgggatttat
     9901 gctaaaccaa tcgtttatcc aactgtgcct cttgggacag gcaggatcag aaatatgccg
     9961 actgcggaac atacgaggga aatgcttgat gaagcagtag cagtttatca aaaaatagga
    10021 aaagaaatgg aaattattta actaaattta gttttttcgt ctatttaaaa ttagagaaca
    10081 tgttcacgca tatattgtta atcatctgaa gatagtagta aaaaagatca gggaggatca
    10141 tcatgaaaaa agtattggtg acaggatgtt taggacaaat tggttcagag ttgatcacac
    10201 ggctacgtca agaactaggc agtggaaatg tcattgcgac agatatacgc atgcctgaag
    10261 tcattgagaa aaacgatatc tttgagatcc tagatgtaat ggattacgaa aaaatgcgca
    10321 gcatagcaga acaatatcaa gtggacacat tgattcattt ggctgctctt ttgtcagctg
    10381 ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt gactgggctg gtcaatgcat
    10441 tggaagtcgc tagagaattc cagctgaaat tttttacgcc aagttcaatc ggcgcgtttg
    10501 gtccaaatac tccaaaagat cataccccac aagatactct tcagcgtcct acaaccatgt
    10561 atggggtaac gaaggtagct ggagaactac tatgtgatta ttattatgtg aaatatggcg
    10621 tagatactcg tggtgtccgt ttccctggat tgatttctta tgcaaccttg ccaggaggcg
    10681 gtacgacaga ctacgcagtc gatatttatt attcagcaat caaagaagga aagtacacta
    10741 gtttcattga tcaaggtaca tcaatggata tgatgtatat gccagatgcg atcgatgcca
    10801 ttataaaact catgaatgca gatcctgatc gattggttca tcgaaatgcc ttcaatatat
    10861 cggcgatgtc ttttgacccg gaagagatca aagcatctat tcaaaaaatc ataccagatt
    10921 ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat agcagaatct tggccaaatt
    10981 cactagatgt ttcttgtgcg caagaggaat ggggcttttc gccgaaatac gacttagatg
    11041 tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt ggtagagacg gtataaaaca
    11101 agaaccacat ccaaaaagtt ttttatactt ttgggattgg ctatggtatg aaaggggaca
    11161 agggatggaa gaatatcaag ccacgccagt aaaagaagta aaaatagaaa atgaattaaa
    11221 acgtacgatg ggatttttta ctgctttatc gactgttatg ggcacagtga tcggtgcagg
    11281 ggtatttttt aaagccgcaa gcgtagcaga agttacagga tccgccagtt tgcatatgtt
    11341 ttcttggttt ctcggtggta tgatttctgt ctgtgcagga ctaacaggag ctgaactggc
    11401 agctgctatt ccagaaacag gagggatgat taaatatatc gaacggattt acggaaatac
    11461 agcagctttt ttattgggct gggcgcaagt cgtgatttat tttccagcaa atgtagcagc
    11521 cttgtcgatc attttcggta cacagttcgt caatctattt ggtctttccc agtcaatgat
    11581 tgttcccgta gctgttacag ctgctgtatc gatcctgttg atcaattttt taggttctaa
    11641 agctggaggg gcttttcagt cgattacgct tgtctgcaag cttattcctt tgtttgtgat
    11701 cgtcattttc ggtttgttcc gacaagaagg agtagacttc caactgtttc cgatccaagc
    11761 aggagaaaat ctttcatttt tctcagcctt gggagcgggc ttgctagcaa cgatgttcgc
    11821 atacgacggt tggatacacg taggaaatat ttcgggagaa ttgaaaaagc cggcgaaaga
    11881 tttgccaaaa gcaatttctt taggaatcat cggaatcatg atcgtttatt tattagtaaa
    11941 tgcggtgttc ttaagaaccg catctatcga cggagtcgca ggaaacagca atgcggcaag
    12001 tgacgttgca aaaatgattt ttggcggctt cggtggaaga ctggtcacag ttggtatctt
    12061 gatttctgtt tatggaacca tcaatggcta tacccttact ggaatgcgtc tcccttatgt
    12121 tatggcaaaa gaaaacaacc tgccttttag caagctcttt gctaaacttc atgataaaac
    12181 taaagttcct gtagccgcag gtatcttaga gctagtcatc gctattggca tgatgatgat
    12241 cggtggtttt gatacactaa cggatatgct gatctttgtc atttggattt tttatacgat
    12301 ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc gaccatacaa
    12361 agttccaatg tatccgttta ttccactagt agcaatcatt ggagggactt ttatcgtatc
    12421 aagcacgctt atcactcaga cctttttagc ttcgatgggg atagtattga cacttgctgg
    12481 tatcccgatt tatctctatt taaaacgtag atagctaaaa cattcgaatt ttattagaac
    12541 tgcacttctc tgccaaacaa aatattttat ttaaatgagg agtacgtgga agaaatgtca
    12601 aaagtactcg ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa atagcaaagt
    12661 aaaagacaga aatcgggctc aaccgatttc tgtcttttac tttgatttgc gtaactgaac
    12721 tattcggcgt acgccgcgat aaatatttaa taaaaccaag caaccagaaa tgaaaatggg
    12781 aagccaaatc agatagacag ggtagccaag cactttagac aaagcatgtg gagtcatttt
    12841 atccatttca agaagtgctt gctgccaagt ctgaagattg aagactagca tgaaacccga
    12901 aataatcagg agaataaatc ctaaataatg aattgtagtt ctttttctta cataagcgat
    12961 cgtagcataa aggaacagta aaaaaatagg tacgccaaaa acaaagtaca tattcagcat
    13021 ctcgacaccc cctttgtgtt tatcttactt tactttgaaa gaaatgagaa ataatagcct
    13081 cgttacttgc ttgaaacagc aaaggactgc taagataaga gagatacaaa gaatcaagga
    13141 ggagtcatat ggcaagttat atcgaaccga ttcgttttgc gctgattgtt tttccatttt
    13201 tagccttagc gatctcatct gtttttttta tttatgaata tcgaaagtat ggaacatttc
    13261 tagtaacaag ggcagttatt ttgtattctt tcgtttttta tttattgtgt gcgtattttt
    13321 tggtgatctt accacttccg gcaaaggaga cggttgccca aatgacaggg ccaaagatgg
    13381 agctgcattt atttgcttca tggcaacatt tcagagacaa cacggtattg gtcttgtctg
    13441 aacctagtac ctacctgccg gcaatgaagc agagtgtatt tttggaacca gcttttaatg
    13501 ttttattact cataccgttt ggc
//
LOCUS       DACCWV010000075        13428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCWV010000075 DACCWV010000000
VERSION     DACCWV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.075"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            78..245
                     /locus_tag="IVW84_001818"
     CDS             78..245
                     /locus_tag="IVW84_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7918009.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(307..2118)
                     /locus_tag="IVW84_001819"
     CDS             complement(307..2118)
                     /locus_tag="IVW84_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7918010.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2115..2480)
                     /locus_tag="IVW84_001820"
     CDS             complement(2115..2480)
                     /locus_tag="IVW84_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7918011.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2643..3038)
                     /locus_tag="IVW84_001821"
     CDS             complement(2643..3038)
                     /locus_tag="IVW84_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7918012.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3056..4018)
                     /locus_tag="IVW84_001822"
     CDS             complement(3056..4018)
                     /locus_tag="IVW84_001822"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7918013.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4018..4230)
                     /locus_tag="IVW84_001823"
     CDS             complement(4018..4230)
                     /locus_tag="IVW84_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7918014.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4251..4949)
                     /locus_tag="IVW84_001824"
     CDS             complement(4251..4949)
                     /locus_tag="IVW84_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7918015.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4969..5511)
                     /locus_tag="IVW84_001825"
     CDS             complement(4969..5511)
                     /locus_tag="IVW84_001825"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7918016.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5643..6647)
                     /locus_tag="IVW84_001826"
     CDS             complement(5643..6647)
                     /locus_tag="IVW84_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7918017.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6644..7633)
                     /locus_tag="IVW84_001827"
     CDS             complement(6644..7633)
                     /locus_tag="IVW84_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7918018.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7630..8436)
                     /locus_tag="IVW84_001828"
     CDS             complement(7630..8436)
                     /locus_tag="IVW84_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918019.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8602..9558
                     /locus_tag="IVW84_001829"
     CDS             8602..9558
                     /locus_tag="IVW84_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7918020.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9635..10153)
                     /locus_tag="IVW84_001830"
     CDS             complement(9635..10153)
                     /locus_tag="IVW84_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7918021.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10241..10390)
                     /gene="rpmG"
                     /locus_tag="IVW84_001831"
     CDS             complement(10241..10390)
                     /gene="rpmG"
                     /locus_tag="IVW84_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7918022.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10618..11064
                     /locus_tag="IVW84_001832"
     CDS             10618..11064
                     /locus_tag="IVW84_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7918023.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11259..13154)
                     /gene="asnB"
                     /locus_tag="IVW84_001833"
     CDS             complement(11259..13154)
                     /gene="asnB"
                     /locus_tag="IVW84_001833"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7918024.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4187 a         2843 c         2286 g         4112 t
ORIGIN      
        1 caaacaaacg taaaactagc ctttttttct cttttgttcc ataatagata taacaaataa
       61 taaatgaggt ggattcaatg aaaaaatttg tatctggaat attagtagga agtctggcaa
      121 cagccgctgc cgttgctggt ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg
      181 acgaaaaaga agcaatgatc gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc
      241 gttaataatt atttaaaaat acgcatgaag actttctaaa aaacagataa gtcatcatgc
      301 gtatttttat ttgtcagtca atataagttg tgctatttta tctgcctgtc tcagattcca
      361 tggatctgta taaggtctag ctactgctgc agttagccaa tccacacctt caacaggtat
      421 tccatagcaa taaaagtcag actggatttc ccctgaagca tcaatcacgc gattcgtacc
      481 tcgctcaaca tctatagctt ctgaatgata acttcctgta ttatctgaaa atgaaaaagg
      541 gcgaacaagt ttgttttcct tcaaattttt tatgacagga ttacttgaac gagaaaagct
      601 tgtttgtggc aatcgagctt caatcaaata gcgactgctg atttctttat cttgtagaga
      661 aataacatag cggttgtgct tttgctgaat atcaaatggc ggttcgatca aatgaaaaat
      721 acccgcatct atcagtgctg ctaattcttt cgtccgaatg attggcggac cgatcgttaa
      781 aaagctattt agatgagtaa accatcccca tagtatttct ttgtattcct tagcagaaaa
      841 aacgtgccat tcaatagcct gccggattgg atcacgccag tcttttaatg catcaaatgt
      901 tgaagcaaat gctcctgtgc aattgccttt ttccgcttct tttatctgcc cttcaataaa
      961 tattcggcta gcttctgaaa agggctcatt ctgataggca agaggagttt ctaaatacga
     1021 ccaatcccat ctataaggga ttagtgctgg atgctttccg agacactcct ctgaagagag
     1081 ggagagaaaa tcatgctcaa aatttttctt tggaatcggc aagtgatatt cttcgatgat
     1141 tttcttgtaa taaaacaact caacgtcttt tttcagatag tcaaaaaatg tttcgccagt
     1201 caaaaatccc gactgatgcc attgctccag gttttctttt gttagtaacc gtggccatgc
     1261 cattgctccg ccttgctttt gatttcttcc tcgagggtaa taagggagcc cccttcttga
     1321 tccactgtat acaatcggtt ccttgcctga tggatgatag accagttttc ccttttcgtt
     1381 ctcgaagcgc cctcctcttg atactgtaaa caatccgaca taatcaaaaa aacttaagcc
     1441 tagacctctt aaaaagacat tttctttagg cggtatcttc gaaagatcta catctgcagg
     1501 attagaagga agctgataca gtaaaacatt tttctttgcg tagtctaccc acactctttc
     1561 ttcttctgtc tcatcatttt cccagtgacc gcttgctaaa acaagctgat cgatagaata
     1621 gctttgctga tctgtctgta aaagataacc atcttttctt ttgcttactt tttttactaa
     1681 agaataaaca aactgaatag aagctggaaa ttcttcttgc aactttgaga aaaaccagcg
     1741 ttgatagagt ccgtataaac atcgagtaga ttgctgatct ttttccagac gattgatttc
     1801 ttctataatt agttgcttat ttttggttac cgtctgttcg atataatctg tagcaggtcc
     1861 tttactccat tgaaacaagt tgggtccctg tgttaccaat cctccactgg acaatgtttc
     1921 atctgtaaaa agcgtcactt gttgagaaac agaattcatt aataactccg gtggctgatt
     1981 caaccgccaa actcgacctc caggaccatt gggatcgaaa agaaaaagtt caagagaatg
     2041 tctgtcctga ttattatgga gaatcctttc tgctacactt aatcctctag gtccggcacc
     2101 aatgatccct attttcattt gcatgcctcc atttcgataa attttttcaa taaaacagca
     2161 tggttctgat gttcattttt tgcaccataa acaaaagtaa tgactggatg ttctttcata
     2221 agttgactga gtttaaaaat agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg
     2281 tatttctttg caaatgaagc aaatcgttct ggtacatggt caaaagtttt gcgtgcttca
     2341 ttactaggtg caatttcttt caaccacaga tctaactgcg ctttttcttt tgaaattcct
     2401 cgtggccaga atctatccac gagtattcta tagccatcct ctttttcagc atcaaggtaa
     2461 acacgtttta atttgatcat agttgtttcc ttctttctac cttttcgctt agcttatata
     2521 tgtagataat agcacaaaat aaaaaaaatg aacggcacaa ctatccattg cgccgttcat
     2581 gtgatcttgg tcaaaatcta atgattgatt cggtttttaa agctgtcata caatcatctg
     2641 ttttatactt ttttactttt gatttttcca gaccatccgt catatccacc cttaagtaaa
     2701 taaatctctt gataacctgc tttacgtaat ttatttgctg tacggatgct caaacttttc
     2761 ttttgatcgt aaatatagac aggctggtct tttcgaatag atcctaatga atctttcaaa
     2821 acagtgtaag ggatgtttct tgcacctaaa atatgcccag catcaaacac atctttttcg
     2881 cgaacatcga ttacttgtgc ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc
     2941 gttgctgaac gtttggccat tactcgtaga tagacttccc aaaggatgat tgcaagcaca
     3001 atcaatagca aaatgatatt aattacccat aaaaccatgt aatgcctccg tttttttaag
     3061 caaatttcaa ggctaatgag gcagcaccaa tgacaccagc ttcattacca agctctgcta
     3121 atttgatttt tgttgattct cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta
     3181 cacggctacg taaaaattct cctgctgccg atactcctcc acctaacacg atacttgacg
     3241 gatttaatgt attccccaag tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa
     3301 caaccatcaa tgcaaacgga tcgtcttctt gagccatttc aaagatatct ttacttgtta
     3361 cttcttctcc gttgtctagc attgctttca gttttgaatc ccctgcgtat tcttctgcca
     3421 agaaacgacc taggcgtacc acacctgtag cgctagatac agtttctaaa catccgcgtt
     3481 taccgcaagt acattcgaac ccttcaggat ccacagtgat atgtccgatt tctcctccag
     3541 ctccgctcac gccgtgaatc aaatttccgc cagcaacgat acctccgcca acacctgttc
     3601 ctagagtaac gaatacaacg tctggatcgt tttctccagc acctttccat cgttcaccta
     3661 aagcagcaac attcgcgtca ttatcgatag cgaatttgat tcctgttcct gcctcgatca
     3721 gttttttcac aaattgaact tctgtccagt ttaagttata cgcaccaata accgttccag
     3781 cttcgctgtc cacgcttcct ggggtaccca tgccgattcc gataaagtct tcggctttca
     3841 tattatataa attcaaacga tgattgattg attcaatgat ttcaggaacg atatgcttac
     3901 catcatccaa aatattggta tcgatgctcc atttttgctg aatctctcct tctggtgtta
     3961 aaattgcaaa ttttgctgtt gttccaccta aatcaatccc aataatcttc ttgtccatta
     4021 caaatttcct ttctttcggc tttcttcaat tcggtgctct ctttttaata tgcgccacgc
     4081 tgtcatgtat gtatcatgat caatcaaacg gctatcatac agattacgca attcgatagt
     4141 cattaattca atgtcataga tgcgtgcacc cacatagata aagatgccaa attttttgaa
     4201 cagctgctgt acatcatata agctctccat ctttaagcac ttccttcata ttatacaagt
     4261 aaaccatatt tttttaaacc cagtaaaata catattacaa aaagaaatac aaaaactagt
     4321 gtagaaagaa tcctttggtg tatcgaatag cttgtctgtt gtttcggtag acccaaaaca
     4381 ttccctaaca ctaatccgcc aattagccca ccgacatgtc cccaaacatc tacagaggta
     4441 tccagaagac caaaaacaaa tgtcaacaca ataaataaaa gaaaatgtct gactagctgc
     4501 tggatcgctg ggttatgttt aaaatgaaac cctaagataa acaacgcacc gaacaaaccg
     4561 aatatcgaag tacttgcacc accagacaga acattcgctt cgttaaaagc aaagcttgct
     4621 gtatttccca ttatcccgct gaacatataa atcaagaaaa aacgccaatg gccatagatt
     4681 gcctcgattt gctgccccat aaaatacagt atcagtgagt tcattacgaa atgcatcagt
     4741 ccaaaatgga tgaagattgg tgtcactaaa cgccaccatt cattgaagtg aacaagataa
     4801 ggaccataca ttccggcata ataagggatc tcaagtcgcg gaaataattc catcatgagg
     4861 aagaccgctg tctgtatccc taaaaaagca taggtccata acggtcgtct taaccatgca
     4921 cgcaatttta tttgctgttg ataattcatg aaaacctccc atttcatttt agtcaataaa
     4981 caattgttgg atcttctgat caaagctctc cggttgccat tgttcattca attgttcacg
     5041 aaaaacaagg ctacatgttt ttttatgaaa attatttaaa taccggtcgt aataaccacc
     5101 gccaaaaccg attcgataac cggctctatt gtagataata cctggtacaa tgaggagatc
     5161 gatcatctcc ggcaaataga gcttctctga ttgtggctca agaacaccaa aactcgaacg
     5221 tacaagaacg gactgttcat ccatttcata aaacgccatc tgacgtttcg gcaaagtttt
     5281 aggtacaact attttctttt gttctttttc tgctcgttgg ataaccggct gtgtatccag
     5341 ttcaaattca ttcgataaag tcgtccctat cacttttgct gacttccagt actcgctggc
     5401 gaatagctgt ttatagattc gctgttcttt ttcctttttc ttgccacttt tggccagatt
     5461 tttcagcttt tcgattcctt tccggcgtaa tcttactttc aattggtcca aaaactcacc
     5521 cttccttctc tcataaaatg gtataggaaa aaaagaaaaa tagcaatcga actttcaaat
     5581 aaatgcataa atagaataaa aagaagacct agaaatgatt ggccttcctt ttttaatgaa
     5641 tttcaactgt tcagcgcgtg tttttgtctg ataaaaagtg catacagaaa atatggcgca
     5701 ccaaaaaggg caatcaaaat tcctgtaggt acttccccac ttggtagaat cactctagca
     5761 accatatctg cactaagcag aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag
     5821 ttattttctt tacgtacgac tagcttcgca gcatgaggag ctagtagtcc tacaaaagat
     5881 aaactacctg tcaccgctac actaccgctt gccaaagcaa cagctaacaa caaaaggatt
     5941 ttttgacttt tagccacgtt aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc
     6001 aatacaatcc tctgtgcata aagtcctcct agcggaatta agataaccgc ccatggaagc
     6061 aatgcccaaa cataaggcca gctcgcaccc cagattgatc ccgctaacca tgcattcaca
     6121 aatccataat tttccttaga taattggatc gtgcatagca atgttaatgc agaaaggccg
     6181 gcattaacgg caataccgga caataagatt cgattcatac ccaaaaagcc atttcgatca
     6241 taggccgcaa gatagacaca gcaagcagct aaaacaccac ctagaaaggc tacaactagc
     6301 aatagaaacg gcagcgaata gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct
     6361 gctccggcat taatgcccaa tatactagaa tctgccaatt catttcttgt tactccttgc
     6421 aagatataac cagaaacgcc aagacaagct cctgctaata aactgatcag taatcgagga
     6481 actcgaaatt ctctaacaat cagcgccaaa gaagatgatt ctttccctat gaacgtggca
     6541 aaaagctgat ccaggtaaat aaaaggcgta ccaatcatta aactacctat agacaggatg
     6601 acaagtacaa aaagcaagaa atagaacgtc caatatattt ttttcaccgt tgttccctcc
     6661 ttacttgtat cagtaaaaac ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt
     6721 caaaaggggg attgatcatc ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa
     6781 ctaaaaaaga gacaggtaga acataacgat agtccgttcc aaccattccg cgaacaactg
     6841 gcggaaccaa cagcccaata aatgaaacag gtccgactaa cgaaacggca atggctgcta
     6901 acactgcaac tagacagaaa acaatcaacc gcgttcgttt aggatgactt ccaagagcta
     6961 atgcatgtgt ttcactcaaa tgaatcaaag atattgcttt tcctaatgaa aaagaaacca
     7021 aaacagcaag tatatataaa ggaaacaata ctgctaattg tttccatgtg atattggcag
     7081 ctccgcctac aaaccaaaaa gcaagatcct gctgcagatt aaagaattgg gtaagtgctt
     7141 ggctgatagc agtaaaaaaa gaactaatag ctacgccagc taaaatcagc tgtaaaggat
     7201 tcatgcccat tttcgaaaac tgcaaaaaac caaatacgag caaagttaca gcaaaagaac
     7261 ctgccaatga cattagaaac acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa
     7321 gggccattgc caacgaagca cccgcgttga ttcctagtaa gcctgaatct gctaaaggat
     7381 ttcttgtcac tccttgcatc aatgcaccgc tcaatgcaaa acaactccct acaagaaatg
     7441 ctcccgccac acgaggcaaa cgaatatttc gtatgatttg gtgactttgt tttgatctat
     7501 caaaatcaaa caacgcctgc caaaaatctg ctttttccac agtcgctgcg ccatttgaaa
     7561 gagcaacaaa aaaaataatc aaaagaacaa aacagaccaa aagcaggagt ctatttttct
     7621 tcatagtcct cataattgat cctcctgctc ttttggcaac aaatcatagg ttagaaaaac
     7681 tggtttattt gttcttggaa gcgtgacaat cgttgcatca atctcaaata tttttttcat
     7741 acactctttt gtgatcactt cttcaggtgt accttctgtg atcaacttac cgtttttcat
     7801 tgctaataaa taatctgaaa aacgtgaagc atgattaatg tcatgaatcg acatcaagat
     7861 cgttgtttgt ttcatttgat tgatttcagt caataactgt aggatctcta attggtgtgc
     7921 cggatctaaa aatgttgtcg gttcatccaa gatcaagata tctgtttcct gagcaagcgc
     7981 catagcgatc cagacacgct gggtttgtcc accagacagc gttgcgatat cccgcatacg
     8041 aaatccagct agacctgtag catccaacgc ccactcaatt gcttcatgat cttgcgtgtc
     8101 catgccactg aacagttttt gataagggta tcgtccgtaa gatactactt cttctactgt
     8161 catacctgta gggtgttcac tagtttgtgc taataatgca attttttttg caagttcttt
     8221 tgaactgtat gtagagatat cagagttatt gacaatgatc tttcctgatt tcacaggtag
     8281 tatacgggta aaacttttca ataaagtaga ttttccactt ccatttggcc caatcaaact
     8341 agtgattttc ccttcaggaa tcgtaacaga catgttttca atgatttttt tgttttcgta
     8401 gccggctgca atgtcagctg ctcttaatct cgtcatttca cccatccttt cgctgaaaat
     8461 cattctcaat tatgacgctg tttcaacatt tcgtcaagcg ctactttcat atctatagcc
     8521 aaatgaagcg gttgacagcg ttcgaaatat caggaataat aaccaatgag aaagattctc
     8581 aattatgtta ggaggaaaga aatgaaatta aaaacaaaag tattcacatt atctagcata
     8641 ttgctgctgg ctggagtact gagcgcttgc tccccctctt cagataaaga aacagctaca
     8701 actgccaatt cgacgacttc atccaatagt actcatacag tcacagatac acttggacat
     8761 aaagtagaga ttccaaacgc gcccaaacgg atcatcggca gttacttaga agactatctg
     8821 attgcactag gggaaaaacc tatcgcccag tggactgttg gcagtggttc cattcaagat
     8881 tatctgcaag atgatttaaa ggatgtccca acgatttctt atgaccttcc ttatgagaaa
     8941 gtgttaagct ttgagcctga tttgctgttg atttcttctt ccgctactgt cgaaggcggg
     9001 aaatacgaac aatatagcaa aatcgcgcca acttatgttg taaaaaacgg aacagatgtc
     9061 acatgggaag aacaactaaa agatgtcggc aaagctttgg caaaagaaaa agaggcagaa
     9121 aaaatcattt ccgattatca aaaatccatc aagaaaactc gagaagaact agcagataaa
     9181 atcgagaaca agacagctgc agtgttgtgg gtgaccaaca actcagcctt tatggtcagc
     9241 aaaaatcggt caagtggaag aatcgtatac gacgatctgg aatttggtgt tcctgatcta
     9301 gtggaagagg tctccaaaaa agccacttct gactggtctg ctgtttcctc tgaaaagcta
     9361 gctgaattag atgctgacta catcatcctt gtgaacagtg acaaagatgc tgccatgttc
     9421 aatgaaccaa gctggaagaa tctaaaagca gtgacagaca atcatgtact gcaattcgga
     9481 cctgaatcta gttggctata taacggacct attgccagtc aggaaatggt caatgatatc
     9541 aaagaaaaac taaattgatt ttaactaaaa aaagcatctt cacgattagt cgtgagatgc
     9601 tttttttctt attaacaaac tagacgcata gattttattc ttcttttgaa gcaaccctat
     9661 tttttactct ttcccatttt tgaaaacgat gggcatatag attttcttga tccgttatcc
     9721 cctcatactc ctctataagg gcgaagtctg aaaaatcaat attcccaata tacgtatcgc
     9781 cttcaaacgt gctatcgatc aatgttcgcc agatccgacc agtgtaagga agaagttctt
     9841 gaaaaatccg actaccaccc gaaacataga tatcttctga gatcgtttga ctatacgaca
     9901 aaagttcatc gatcgaatgc attacttccg cattttcttt cgtgcgaaaa tttggttgcg
     9961 ttgtcaacac gatgatatgg cgataaggaa gtgaaagatc ccccattcct tcataggtct
    10021 ttcttcccat cactaaaatt cgatccattg tatgttcgcg aaagaaacgc atatcatttg
    10081 gcaatcgcca tggtaacaaa ccatttttgc cgatcagacc atttttatct tgtgcccaca
    10141 tggaaataaa catattatcc gctccttatg attttagatg ttttgagata aaaaaagtct
    10201 caattctttg accagcaagg aattgagaac tttaaaaaca ttattttgtt tcacggtgca
    10261 aagtaacttt gcgttcgcgt gggcaatatt tttgtttttc caaacgatca ggattgttac
    10321 gtttgttttt gcttgttaga tagttacgtt ctttacaaga agtacactct aaagtgatgt
    10381 ttacgcgcat gtttttccct cccattttta caagaatcct ctaagatgac tctcagactt
    10441 gattatctta ccatgatttt ttctagattg ctagtgtttt tcacgatttt gttaagtaaa
    10501 actacttgac gataaataca acggttttac ttctcaaaca aaatcttttt ctttttgtat
    10561 aaataccctt acacttgttt atatagatta tgttttttaa taaaggggga tactaatatg
    10621 ccatggaaca tgaaggactt tcctgcatca atgaaaaact tagataaatt gacaagaaaa
    10681 aaagcaatcg acatcgcaaa tgcattatta gatgaagggt accctgataa ccgtgcgatc
    10741 ccaattgcca ttgatcaagc aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga
    10801 actttcgaaa aagaaaaaaa tccaagcaag acggatgaac acgatacgaa tcctcgtgct
    10861 ggaaaactgc ttgattcaga tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa
    10921 tctaaagggg caaaaaaggc aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa
    10981 ggaaagcaag tagcacaaaa caaagaatca actttgttca tttataaaaa agatgggaca
    11041 aaagaaaaag aaatcgatta ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac
    11101 aaccccctta tgttcgaaac aatggtgttc ctctgccaac tccgaaccat aagtcataat
    11161 atctataaaa cgggaagagc tgttttttga aattgtttct tatcactcag tgttaaacgg
    11221 taggagaaaa cctctttttt attgatattt gattttcatc aaacagtcac atagtcgatt
    11281 gcttctgctt gcggaatcgt ttcatctaca aagtagactt tataccatgt caagaaactg
    11341 aagattgtcc agatttttct ttgctcttct ttacgtcctt cgtgatggtc atctagcaac
    11401 ttcatgattt tttcttgatc aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg
    11461 acttgttcgt agccgtgttc ttctttcaac caagctttga ttggcacagg gaatcctaat
    11521 ttcacacgat tcgaccattc ttccggaaga tgtttgttcg cagcttgtct gaaaatatct
    11581 tttgtgttgt tttgattcaa taaaaactta cttggtattt gttgagccaa tttcatgact
    11641 tcgatatcca ataaaggtac tcggacttcc aaagaacttg ccatagaaag tttatctgcc
    11701 tttaacaaga tatcttttgg catccattga tgaagatcga catattgcat cttattgact
    11761 tcatctttga ttccttctgt tttctcgtaa tgaacagtca tgatttcatc cacagatggt
    11821 gctgtttgat attttggtgt taacacttct gatgcttctg attcttcgaa cactcgagca
    11881 tgaccgataa aatattcttt tgcaggagca agtgattcat ataaatggat ccgtccatgg
    11941 aagtttttca tttttccgat tttacgtcct aaattatagc gtgttccttt tggtaatttt
    12001 ttcaatcctt gagcaatcac acggatgaat tttgaattcg tatggaatcc atacgtttgg
    12061 tatcccgcaa acagttcatc cgccccttct ccagattgga ctacccgcac actttgtgaa
    12121 gcaagatttg ctaaaaagta caaaggtacg caagaaggat ttgaatcagg ttcatctaag
    12181 tgatactgga tcaaagggaa tgctttgaac gacatgtctc cgtcgatcac tgctgctgta
    12241 ttatctaagt ccaatagttc ggttagtttt ctggcttcaa tcgcttcatt ataggtttta
    12301 tcatcaaacc cgattgagaa agaatgatct ggtttcaata cagaggttac ataactggaa
    12361 tcaaccccgc tggataagaa agaaccaact tctacatcac tgaccgtatg tgcttcaata
    12421 gaagcttgaa cggtttcgtc gattttttcg atccattctt gtcgggaatg cgtttctttt
    12481 gtttcaaaat ctgcatccca atattgctga atgtctattt ttccatcttt atacgtataa
    12541 taatggcctt caggcaaacg atagacatct ttgaagaatg tttcgccgtt taatggagaa
    12601 tattggaaag tcatataagg tttcaacgct tctttgttca attctttgtt aaaatcagga
    12661 tgaggcagaa aacttttgat ttctgaacca aacataaatg ttccgttcat ttctgcataa
    12721 taatatggtt tgatcccaaa gtggtctctt gcaccgaata actcattctt ttcattatcc
    12781 caaatagcaa aagcaaacat tccgcggatc ttttgcaata attctgttcc ccattcttcg
    12841 taaccatgca aaaggacttc tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc
    12901 aattcttctc tcaaaggctg gtaattatag atttcgccat taaaaataat gatctttgtc
    12961 cgatcttcat tgtaaatagg ctgagtaccg ccctctaagt caataatact taatcttctg
    13021 aatcctaaag caacatgttt atcgatatat tctcctgaac tattaggtcc acggtgaacg
    13081 attcgatcca tcattgtatt aataattgtt tttttgttgt ccttgtcgtt aacaaaacct
    13141 acaataccgc acatttaact ttcacatcct aatataaaat tactgtttat ttcattcata
    13201 tgcatcttat catagaataa atagcggacg aatattttct ctcttacaat ttcgtcacgt
    13261 gaaaacaaaa gatgaaagtt tctgttttta cagaaaataa agtcccaata tctaatttac
    13321 catgttttct tttttgaggc taccttattc tcttcgtgtt aaaaaaatat tagtggatgc
    13381 tatttccaaa aaaaccagca aaaaaagaca cgacctaaag atcgtgtc
//
LOCUS       DACCWV010000076        13362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCWV010000076 DACCWV010000000
VERSION     DACCWV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.076"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="IVW84_001834"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="IVW84_001834"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7918025.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="IVW84_001835"
     CDS             1013..2260
                     /locus_tag="IVW84_001835"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7918026.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGNGQIRE"
     gene            2336..2680
                     /locus_tag="IVW84_001836"
     CDS             2336..2680
                     /locus_tag="IVW84_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918027.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="IVW84_001837"
     CDS             complement(2797..3123)
                     /locus_tag="IVW84_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918028.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="IVW84_001838"
     CDS             complement(3496..4698)
                     /locus_tag="IVW84_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7918029.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="IVW84_001839"
     CDS             4815..5330
                     /locus_tag="IVW84_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7918030.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="IVW84_001840"
     CDS             complement(5529..5975)
                     /locus_tag="IVW84_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7918031.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr"
                     /locus_tag="IVW84_001841"
     CDS             complement(6022..7500)
                     /gene="msr"
                     /locus_tag="IVW84_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7918032.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="IVW84_001842"
     CDS             complement(7854..8483)
                     /locus_tag="IVW84_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7918033.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="IVW84_001843"
     CDS             complement(8467..8931)
                     /locus_tag="IVW84_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7918034.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="IVW84_001844"
     CDS             complement(9418..10686)
                     /locus_tag="IVW84_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7918035.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="IVW84_001845"
     CDS             complement(10917..11654)
                     /locus_tag="IVW84_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7918036.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="IVW84_001846"
     CDS             complement(11721..12290)
                     /locus_tag="IVW84_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7918037.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="IVW84_001847"
     CDS             12418..13290
                     /locus_tag="IVW84_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7918038.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         4032 a         2752 c         2423 g         4155 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggca atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa ta
//
LOCUS       DACCWV010000077        13274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCWV010000077 DACCWV010000000
VERSION     DACCWV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.077"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(327..399)
                     /locus_tag="IVW84_001848"
     tRNA            complement(327..399)
                     /locus_tag="IVW84_001848"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Lys,seq:ctt)
     gene            780..1349
                     /locus_tag="IVW84_001849"
     CDS             780..1349
                     /locus_tag="IVW84_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7918039.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1336..2196
                     /locus_tag="IVW84_001850"
     CDS             1336..2196
                     /locus_tag="IVW84_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7918040.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2386..3090
                     /locus_tag="IVW84_001851"
     CDS             2386..3090
                     /locus_tag="IVW84_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918041.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3095..4930
                     /gene="walK"
                     /locus_tag="IVW84_001852"
     CDS             3095..4930
                     /gene="walK"
                     /locus_tag="IVW84_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7918042.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4927..6243
                     /locus_tag="IVW84_001853"
     CDS             4927..6243
                     /locus_tag="IVW84_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918043.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6244..7113
                     /locus_tag="IVW84_001854"
     CDS             6244..7113
                     /locus_tag="IVW84_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918044.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7168..7977
                     /locus_tag="IVW84_001855"
     CDS             7168..7977
                     /locus_tag="IVW84_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7918045.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8080..8709)
                     /locus_tag="IVW84_001856"
     CDS             complement(8080..8709)
                     /locus_tag="IVW84_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP7918046.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8879..10171
                     /locus_tag="IVW84_001857"
     CDS             8879..10171
                     /locus_tag="IVW84_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918047.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10247..10909)
                     /locus_tag="IVW84_001858"
     CDS             complement(10247..10909)
                     /locus_tag="IVW84_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7918048.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11134..11418
                     /locus_tag="IVW84_001859"
     CDS             11134..11418
                     /locus_tag="IVW84_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7918049.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11469..13094
                     /gene="groL"
                     /locus_tag="IVW84_001860"
     CDS             11469..13094
                     /gene="groL"
                     /locus_tag="IVW84_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7918050.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4415 a         2287 c         2851 g         3721 t
ORIGIN      
        1 aaaaggggaa agcagcaagt aaatttagtt tttctattaa atattcttca ctttttaacc
       61 ttttatagta cttagattta atcctatatg tttagagagt tcattggcag catgattttg
      121 caaagcttct cgaattattg ttatattaga aactttactg ttcataatta tgcttctttt
      181 ttaactcaaa gtccaaaggg ctacagtgga ctttgagtta aaaataaaaa gccctagtta
      241 ttttgaaaaa taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt
      301 acctgttccg tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg
      361 attaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat
      421 agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta
      481 cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac
      541 caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt
      601 tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc
      661 ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga
      721 ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga
      781 tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc
      841 ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg
      901 atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg
      961 taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag
     1021 aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa
     1081 tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat
     1141 tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc
     1201 atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt
     1261 tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa
     1321 aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat
     1381 gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat
     1441 aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg
     1501 ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag
     1561 caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc
     1621 gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta
     1681 ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg
     1741 ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga
     1801 acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt
     1861 ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa
     1921 gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg
     1981 gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat
     2041 ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa
     2101 gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga
     2161 ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt
     2221 gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca
     2281 ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg
     2341 gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta
     2401 gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc
     2461 tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa
     2521 cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt
     2581 gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt
     2641 gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac
     2701 cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa
     2761 ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct
     2821 tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc
     2881 ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt
     2941 tggggttatg attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt
     3001 gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt
     3061 tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg
     3121 atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat
     3181 tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga
     3241 tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga
     3301 taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac
     3361 tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga
     3421 tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac
     3481 gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc
     3541 gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga
     3601 acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc
     3661 ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg
     3721 tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt
     3781 gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat
     3841 tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca
     3901 tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat
     3961 ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact
     4021 tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt
     4081 acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc
     4141 tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac
     4201 agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact
     4261 tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa
     4321 agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat
     4381 tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca
     4441 attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat
     4501 ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg
     4561 ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat
     4621 caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt
     4681 gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt
     4741 cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg
     4801 attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa
     4861 ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg
     4921 gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta
     4981 agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta
     5041 gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt
     5101 cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat
     5161 ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta
     5221 gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg
     5281 gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat
     5341 gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa
     5401 ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt
     5461 tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa
     5521 atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg
     5581 aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc
     5641 cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg
     5701 aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca
     5761 gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc
     5821 agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata
     5881 ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt
     5941 ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa
     6001 gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag
     6061 ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg
     6121 caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat
     6181 aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat
     6241 taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg
     6301 tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat
     6361 cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt
     6421 tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca
     6481 ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac
     6541 ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca
     6601 gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt
     6661 aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa
     6721 gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct
     6781 ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat
     6841 atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg
     6901 tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc
     6961 tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa
     7021 acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca
     7081 aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta
     7141 caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt
     7201 atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt
     7261 ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg
     7321 acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc
     7381 gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa
     7441 gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg
     7501 aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg
     7561 gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc
     7621 ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa
     7681 agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt
     7741 attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg
     7801 atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa
     7861 gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat
     7921 gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca
     7981 gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt
     8041 ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat
     8101 gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc
     8161 catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata
     8221 agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg
     8281 tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata
     8341 gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa
     8401 taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat
     8461 caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc
     8521 aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat
     8581 aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc
     8641 tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc
     8701 aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc
     8761 gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt
     8821 gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat
     8881 gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc
     8941 cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct
     9001 gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg
     9061 attttctatg tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat
     9121 cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg
     9181 cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac
     9241 tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg
     9301 gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga
     9361 aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct
     9421 tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac
     9481 atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg
     9541 tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt
     9601 ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca
     9661 agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat
     9721 gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa
     9781 atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa
     9841 tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat
     9901 tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc
     9961 tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt
    10021 aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta
    10081 tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa
    10141 tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat
    10201 tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat actagccctt
    10261 tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg
    10321 atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt
    10381 agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc
    10441 cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact
    10501 cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca
    10561 tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta
    10621 acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa
    10681 caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct
    10741 taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga
    10801 tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa
    10861 ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc
    10921 ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata
    10981 atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa
    11041 agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac
    11101 agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc
    11161 attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct
    11221 gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa
    11281 aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac
    11341 gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc
    11401 atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg
    11461 aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg
    11521 gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg
    11581 tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag
    11641 aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat
    11701 caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa
    11761 tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg
    11821 ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg
    11881 attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac
    11941 atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat
    12001 caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact
    12061 tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata
    12121 tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa
    12181 tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc
    12241 caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg
    12301 gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag
    12361 taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg
    12421 cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag
    12481 aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt
    12541 ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta
    12601 aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat
    12661 tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag
    12721 tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca
    12781 agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg
    12841 aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg
    12901 ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac
    12961 gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg
    13021 ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg
    13081 gcggtatgat gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag
    13141 ataaaaagag gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa
    13201 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc
    13261 tgtttatacg tgtt
//
LOCUS       DACCWV010000078        13102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCWV010000078 DACCWV010000000
VERSION     DACCWV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.078"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..608
                     /locus_tag="IVW84_001861"
     CDS             <1..608
                     /locus_tag="IVW84_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918051.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="IVW84_001862"
     CDS             628..1656
                     /locus_tag="IVW84_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7918052.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="IVW84_001863"
     CDS             1653..2411
                     /locus_tag="IVW84_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7918053.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="IVW84_001864"
     CDS             complement(2497..3237)
                     /locus_tag="IVW84_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918054.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="IVW84_001865"
     CDS             3298..4551
                     /locus_tag="IVW84_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7918055.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="IVW84_001866"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="IVW84_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="IVW84_001867"
     CDS             5222..5548
                     /locus_tag="IVW84_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7918056.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="IVW84_001868"
     CDS             5616..6329
                     /locus_tag="IVW84_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7918057.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /locus_tag="IVW84_001869"
                     /pseudo
     CDS             6639..7491
                     /locus_tag="IVW84_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7612..9477
                     /locus_tag="IVW84_001870"
     CDS             7612..9477
                     /locus_tag="IVW84_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918058.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9525..10934
                     /locus_tag="IVW84_001871"
     CDS             9525..10934
                     /locus_tag="IVW84_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7918059.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11042..11743
                     /locus_tag="IVW84_001872"
     CDS             11042..11743
                     /locus_tag="IVW84_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7918060.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11902..12381
                     /locus_tag="IVW84_001873"
                     /pseudo
     CDS             11902..12381
                     /locus_tag="IVW84_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4407 a         2191 c         2757 g         3747 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tt
//
LOCUS       DACCWV010000079        13011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCWV010000079 DACCWV010000000
VERSION     DACCWV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.079"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            95..2260
                     /gene="acm"
                     /locus_tag="IVW84_001874"
     CDS             95..2260
                     /gene="acm"
                     /locus_tag="IVW84_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7918061.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2532..3017)
                     /locus_tag="IVW84_001875"
     CDS             complement(2532..3017)
                     /locus_tag="IVW84_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7918062.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3497..4138
                     /locus_tag="IVW84_001876"
     CDS             3497..4138
                     /locus_tag="IVW84_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7918063.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4157..4792
                     /locus_tag="IVW84_001877"
     CDS             4157..4792
                     /locus_tag="IVW84_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7918064.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4810..5625
                     /locus_tag="IVW84_001878"
     CDS             4810..5625
                     /locus_tag="IVW84_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7918065.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5944..7380
                     /locus_tag="IVW84_001879"
     CDS             5944..7380
                     /locus_tag="IVW84_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7918066.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7569..10016
                     /locus_tag="IVW84_001880"
     CDS             7569..10016
                     /locus_tag="IVW84_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7918067.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10246..11313
                     /gene="rlmN"
                     /locus_tag="IVW84_001881"
     CDS             10246..11313
                     /gene="rlmN"
                     /locus_tag="IVW84_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7918068.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11506..12609)
                     /locus_tag="IVW84_001882"
     CDS             complement(11506..12609)
                     /locus_tag="IVW84_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7918069.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4442 a         2175 c         2759 g         3635 t
ORIGIN      
        1 gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga tatagtaggt
       61 tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa atattatttt
      121 atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg tctatgcgga
      181 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
      241 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
      301 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
      361 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
      421 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
      481 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
      541 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
      601 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
      661 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
      721 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
      781 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
      841 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
      901 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
      961 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
     1021 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
     1081 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
     1141 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
     1201 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
     1261 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
     1321 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
     1381 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
     1441 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
     1501 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
     1561 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
     1621 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
     1681 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
     1741 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
     1801 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
     1861 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
     1921 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
     1981 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
     2041 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
     2101 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
     2161 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga tagtaactat
     2221 ctgtgctggg atattcttac aaaaatataa aaaagtttag ttagaatata atattttttg
     2281 ttaaatatga ctgaaatcaa aagaataaaa ttccgattta taatcgaaaa aagaagcatt
     2341 tttatatatg cttctttttt cgtagaatca ataaatatgg atttggctaa ctatggaaga
     2401 atactcgtat tagttttcag cgttcatact agttgttaca ttgtttgatt ggccaaaaga
     2461 atttctgata tattttgtgc taaataaaga aaaagacgct tgaattggga aataagaaaa
     2521 ctactctatc attacaaatt ttcataaact ggcagatgtt ctaagtaaac aagtatcttt
     2581 tcaaagtcgg aaaaatccat aatcaacgta gtggtattga tatttggatg aaagcctacg
     2641 gtatcttgac gatcaatagc agcatcgatc acgacttgga tttttttctc tttgtcgtgc
     2701 atcaatgcta atggagtcac tgtacctgct ggaacgccta ggaggtcagg aagctgttct
     2761 tcagaaacaa aagataagcg cttttctttt aattgttcag ctagttgttt taaatcggcg
     2821 tgcttttcat caggcaagat gacaagatag atttgttttc cttttcttga tttcaataat
     2881 agatttttta cttgcggacc aggtagggaa aaagggacat ttttgactga cgtaatcgca
     2941 ggatgatcga ctttttgaaa agagataccc agttttttta gtagttcata agcttcttgt
     3001 tcggttgcac gactcatcgc gcatcaccct ccttttttta ttagtgtatc ataaaaaaga
     3061 gttgacaaat gaaaaataat catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa
     3121 aagaagagta gccgttaggt gtttttagag agtctctggt tggtggaaag aggcagcagt
     3181 taaccgtgaa acacatcttg gagtcgattt tctgaagcgt atagtaggaa aatccgggga
     3241 gcccgttaca gctgcggtcg ttgctagatg accagataag gctggctttt ccgtgaggag
     3301 tcggtaaaaa aaggtggaac cacgtgatta acgtcctttg atcttcggat caaaggactt
     3361 tttgttttca tctgaacggc aacgactgtg tgaggttttt tctgcaagga aaaaataaac
     3421 agaggtggaa ccacgtataa tacgtcctct tggcatttgc caagaggatt tttttatttt
     3481 aaaggaggaa aagaagatgg agtatttgat tgaagttatg ccgcaattat tagtaggagc
     3541 actcacaaca ttgaaattat ttttatttac cttgattggc tcattaccat tagggattgt
     3601 tttagcatta ggattgtctt tgcattttaa gccgctacgc tacttgcttc aaacatatgt
     3661 atgggttatg cggggaacac cgctgttgtt gcaactgatt tttatttttt atgggttgcc
     3721 gacgattggg atcgtttttg atcgtttcga agcagcactc attgccttta tcttaaacta
     3781 tgcagcttat tttgcagaga tctttcgtgg agggatacaa tcgattcctc aaggtcaata
     3841 tgaagcagcg caagttttgc aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc
     3901 acaagtgaca aagattgttt taccttctgt agggaatgaa gttatcaact tggtcaaaga
     3961 ttcttccttg atctatatat taggtttagg tgacgtaatg cgtgctggga aaatcgcgat
     4021 ggaacgcgac gcgactttac tgcctttgct aggcgtggca atcatctatc ttacattgac
     4081 tggtattcta acaatcgtaa tgaaacaagt tgaaggaaaa ttaaattatt accgctaact
     4141 gataggagga agaaatatga ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa
     4201 aataatcaaa gatttgaatc taatcattcc agatgggaaa acattagcta tcgtaggtcc
     4261 ttccggtggc ggtaaaacca cgcttttacg tatcttggct ggcttagaaa gttctgacag
     4321 cggagaattt tatttagacg gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca
     4381 agttgtagga gtcgtattcc aagacttcca gctatttcca catctgaata tttttgaaaa
     4441 cattacctta gcaccaaaaa tggtattgaa acagacaaaa gaagaataca ccaaaaaagc
     4501 agaagaatta gcggcttcat tagggattag tgatttattg aatatctatc cttatcagct
     4561 ttctggcgga caaaaacagc gggtagcgat tgccagagca ttggctatga atccacgggt
     4621 attggcttat gatgaaccaa caagtgcttt agatcctgaa ttacgccagc aagtcgagcg
     4681 tttgatccta tcattgaaag aagatggggt aacccaaatt attgtcaccc atgatttagt
     4741 atttgcagaa aatgtcggtg atcaattact aaaagtttcc ccaattcatt aagaaaggag
     4801 aagatagaga tgaaaaaaac cagttttcta ttcgctttga tcgcaggtct tcttcttttt
     4861 gcaggctgtt cgaacaaaaa aacttcagca gataattggg cgactatcaa cgaagagaaa
     4921 aaaatcgtta taggtttaga cgatacattt gttcctatgg gatttagaga taaagacggg
     4981 aagctcacag gatttgatat tgatttagcc aaagcagtct ttgaacaata tgggattacg
     5041 gtagactttc agcccattga ctggtcaatg aaggaatttg aactgaacaa tggaacgatc
     5101 gatttgatat ggaacggcta ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca
     5161 aaaccttata tggaaaacga tcaagtgctg atcacaccta aagaatcagg aatcacaaac
     5221 tttggacaaa tgaataataa gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta
     5281 tttacgaaac aaccagaagt attaaaagag atagtcaaaa accaagatgc tgttttgtat
     5341 gatagtttta atgaagcatt gatcgatctg aaaagcagac gggtcgatgg tctgctgatg
     5401 gataaagtat acgcagacta ttatttgaaa caaagaaacg aactagaaga cttcaacata
     5461 acaaaaggtt cttatgagag tgaggatttt gcagttggtg cacgcaagtc agatagtaca
     5521 ttagtgaaaa aaatcaatga agccctagat caactagaga aaacaggtga attccaaaaa
     5581 atatccgata aatggtttgg cgaagatgtt acgccgagaa attaacaaat aggggactgt
     5641 gacaaaagac gtgtcacagt ccctttttcc gaataaacag tgttctcctg ccaactcctt
     5701 gtgactcgga gctgaatggc aggctcacaa cctcttggta aacggtgttc aaaagccagt
     5761 ttttcggtat taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc
     5821 ttactactcg aaactaagcg actttttcac aacctccttt acataatttt attttgttgt
     5881 ggaaggggtt tcgtgttatt ctaaaagagg aacgattgaa ggttatagga aaggaagaga
     5941 tgaatggaac acaaagaact tacctcattt ccaaaagatt ttctgtgggg ctctgcttct
     6001 gcagcgtatc aagtagaagg agcttggcaa gaagatggga aaggagaatc agtctgggat
     6061 cgatttgtac ggatacctgg aaagacattt aaaggaacga acggtgattt ggcagtagac
     6121 cactatcatc gttataaaga agatattgct ttaatgaaag aacaaggtct gaaagcttac
     6181 cgcttctctg tagcttggac acggattttt cctaatggac gaggagaagt caatcaagct
     6241 gggctcgctt tttatgagag attgatcgac gaattgattg aaaacgatat tgaacctatg
     6301 ttgactttat atcattggga tcttccacaa gcattgcaag atgaatataa tggttgggaa
     6361 tcacgtcaga tcattgaaga tttcactcat tatgcagaaa cattatttga ggcattccgc
     6421 gggaaagtcc attactggat tagtttgaat gaacaaaata tcttcacttc attaggttat
     6481 ttgttagcag cacatccgcc aggtgtgaca gacccgaaac gaatgtatga agttaaccat
     6541 attgctaatt tagcaaatgc atctgtcatc aataaatttc acgagttaga aatgcctgga
     6601 aaaatcggac caagctttgc gtatacgcca aattatccaa ttgacagtaa tcctgaaaat
     6661 gttctggctg ctgaaaatgc agaagacctg atggccaatt attggatgga tgtctatatg
     6721 tggggaaaat atccgattgc tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg
     6781 attgaagctg gcgatgcaga attgctagaa tctgctaaac cagattttct tgggatcaat
     6841 tactatcaaa cggcgacgaa tgcattcaat ccattaaacg gagttggtgc tggaaaaatg
     6901 aatacgacag gaaaaaaagg ttcatcagaa gaaactggag ttcctggaat gtataaaaaa
     6961 gttgagaatc catttgtcga acgaacaaat tgggactggg agattgaccc agaaggatta
     7021 aggatcggac tgcgtcgtat cacaagccgt taccgcatgc cggtactgat tacggaaaat
     7081 ggcttaggag agtatgacaa gttgacagaa gataaacaaa tccatgacga ctatcgaatc
     7141 aattacttac aaagccacgt caaagccatc aaagaagcaa tttcggatgg agcagaagtc
     7201 ctaggctatt gcacctggtc ttatacagat ttgcttagct ggctgaacgg ttatcaaaag
     7261 cgatatggat ttgtatatgt tgatcaagac gaaacacaag aaggatcttt ggaacgatat
     7321 aagaaagaca gttattactg gtaccaaaaa ctgattgaag aaaatggaga gaacctttag
     7381 ttcacatact taaaacaaga aggtacggct ggatatggcc gtgccttttc ttgataaaat
     7441 catttagaaa ttaagaaaaa ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct
     7501 ttcaaacaaa actttagttc ccttactttt ttttgtataa tgaaacttga tgaaatgagg
     7561 gataataaat ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga
     7621 aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg tgttgggttt gccttgattt
     7681 tatttgctgt cttcggcttt ttaaaattag gttttttagg aatattgatt gcaaacggct
     7741 ttcggatcat tgcaggaaat acttaccaaa tcctctgtct tttattagcc gtattaggat
     7801 tttggattgt cattaagaat acagagtttt caattggtaa aaaccgacgc tggtttggcg
     7861 gcatactctt ttatttcggt atcttattat tgttacatgc ccatttattt ggcaaactcc
     7921 atactgggga accaaatatc atggggacga catgggattt gttagccagt gatataaaac
     7981 aaagtcaagt agacaataac gttggtggtg ggatgattgg agcaatcctt tatcatttta
     8041 cttacttctt gattgcacag cctggaagtt atttagcagc agtgctatta ctggcaggag
     8101 gagcattcct gttcagtaat ctggaaggct atcagctgtt aaatggtatc caatcaattg
     8161 gagaacgagt acaggaatta ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg
     8221 caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa
     8281 aagaagctgc agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa
     8341 gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt
     8401 ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac
     8461 cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg
     8521 acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac
     8581 cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa
     8641 ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg
     8701 tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg
     8761 taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag
     8821 atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg
     8881 gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata
     8941 aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt
     9001 cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta
     9061 atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga
     9121 tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg
     9181 tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag
     9241 aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg
     9301 ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga
     9361 tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta
     9421 ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc
     9481 caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg
     9541 cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct
     9601 tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag
     9661 gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag
     9721 aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag
     9781 aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg
     9841 caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag
     9901 tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag
     9961 tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact
    10021 tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa
    10081 aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt
    10141 tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc
    10201 atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc
    10261 atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa
    10321 ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa
    10381 atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct
    10441 ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc
    10501 cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt
    10561 gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa
    10621 aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat
    10681 tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag
    10741 ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga
    10801 ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag
    10861 gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat
    10921 gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa
    10981 gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg
    11041 aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag
    11101 aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat
    11161 tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta
    11221 aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga
    11281 agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac
    11341 cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc
    11401 tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt
    11461 cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt
    11521 atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt
    11581 aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt
    11641 atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg
    11701 aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat
    11761 catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt
    11821 ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt
    11881 aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc
    11941 atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg
    12001 atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc
    12061 acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa
    12121 gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt
    12181 tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt
    12241 gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc
    12301 accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc
    12361 tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc
    12421 gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc
    12481 tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac
    12541 gattccattt gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact
    12601 ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat
    12661 cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat
    12721 aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa
    12781 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
    12841 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
    12901 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
    12961 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga a
//
LOCUS       DACCWV010000080        12902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCWV010000080 DACCWV010000000
VERSION     DACCWV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.080"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            129..3656
                     /locus_tag="IVW84_001883"
     CDS             129..3656
                     /locus_tag="IVW84_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7918070.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3653..7375
                     /gene="addA"
                     /locus_tag="IVW84_001884"
     CDS             3653..7375
                     /gene="addA"
                     /locus_tag="IVW84_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7918071.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7444..7785
                     /locus_tag="IVW84_001885"
     CDS             7444..7785
                     /locus_tag="IVW84_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918072.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8094..9137
                     /gene="pheS"
                     /locus_tag="IVW84_001886"
     CDS             8094..9137
                     /gene="pheS"
                     /locus_tag="IVW84_001886"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7918073.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9142..11562
                     /locus_tag="IVW84_001887"
     CDS             9142..11562
                     /locus_tag="IVW84_001887"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7918074.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IVW84_001888"
     CDS             complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IVW84_001888"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7918075.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4381 a         2242 c         2739 g         3540 t
ORIGIN      
        1 tgcgtatact taaaaaagta gaaatatcag aaccttaaaa ggatataatt tgaagaatat
       61 agaacaaatg ttcagtattt tttgatataa taaaggagca ccgtatcatg tgttgaagga
      121 ggaaaagcat gtctttacaa tttattcttg gaaacgggaa aaaagatcac cgtcaagtct
      181 tgatcgacga agcatccgct tggctcgacc aggatgcaag taaccaagta tttttccttg
      241 ttcccaacta cagcaaattc gaacaggaac aagaaatttt agcggagatg cgaagaagaa
      301 gcggaaaaaa aactttcagc acaacaaacg ttcaagtatt tagtttttat cgtctggctt
      361 ggtatttgct gcaacagaca actcttctta caggaaatga attatcagaa tcaggatctg
      421 caatgatctt gcgacagatt ttagaaaaaa atgaggaaga attaacaatc tttcgcggag
      481 aaatcaataa aagagggttt attcgtcaat tacaagaact ctacagtgaa ttgcgtacag
      541 gaaatattca accagaagac ctgatgcttt tatttggtga ttcacctaat gcagaagacc
      601 agcaacttaa aatgaaagac ttgaagctga ttttttcagc ttatgacagc gaattgactc
      661 aacgtgcact aacaaatgaa gatgcactat ctatactagc aaattatatg cagacacaag
      721 atttcaacaa cgtcaaattt attgtcagtg gtttttctcg aatcaatggt caagagtatc
      781 aattactgca ggcaatgatg gaaaaggggc aattggttgt cgatttattg ctggatcgtc
      841 cttatacagc ggatcttcca gaagtactta cgctctttca tgagactgga aagctttatt
      901 tccgtctttt tcaaagtgca agagaacgga aagtccctat attagtggac cgatttgcac
      961 cagagcagac tcgtccgaca gagcttcaac tgctgcaaac aatgtgggaa gagacatcta
     1021 gccaaaagaa agcggcagta cacaaaatag ggggacaaga gcatctccat ttgtgggaag
     1081 ctgaaaatcc aactgaagaa gtccgacaat tagctgttga aatcagacga ctagtcagtg
     1141 aagaaaacta ccgttatcgt gatatccaag tgctgacaaa aaatatgtct ttatatggaa
     1201 atatccttcg gccgatcttt caagaaatgg caattccttt ttatatcgat gaagaacaaa
     1261 tgatggaaaa tcatccgctg atcgaatgga tcaacagtct gttcgctctt gaccgctatt
     1321 cttatcggct aagtgatatt atgcgttttt tcaaaactga attatttctg tcgacggaag
     1381 cagaaaccga acttgatgaa tggaaacaag agcgagatga attcaggaaa aaactggaca
     1441 tcactgaaaa tgtagcactt gcttacaatt atcaaggaac ttattggact cgtgaaaaag
     1501 actggcagtt tgtggcgtat gattttgaag cagaacagat tgaagacgta cagtatttgg
     1561 aagaacagtc aaatacgatt agacgattat ttcagaaagc tgtaccagta tttttgaaaa
     1621 aaataaagaa aagcaaaaca ggacaagagg ccgcaaatgt tttttatcaa tttttgatcc
     1681 aaagtggcgt acagcaacaa ttattgtttt ggcgtaatca ggaagtagac agaggaaact
     1741 tagaagcggc aagaaaccat gagcagacat ggggagcgct gattgatctg ttggatgaat
     1801 atgtagtaat ttatggtgaa tcggcttttg actggtcact gttccaagaa atcattacaa
     1861 gtggtctaga aaatctaagt tatggtaaaa tccctaccgc aatcgaccaa gtgcgaatca
     1921 atcgtttgga attagttcga ccaaatcaag ctgccgtcac ttttgcatta gggttgaatg
     1981 atcaagtgtt tccagaccga aaagagacaa aaggattgtt gtccagtgaa gaacgagaag
     2041 tattaaatca ggccttgcca gatggacagt ttttattcga cccatcaaag gaaagtatct
     2101 cttttgagcc tttccaagcc tatcttgttt ttctttccgg aacagatcga ttatatctta
     2161 gttatgcaca aagctatgac acagatggtt cgctgaagat gtctccttat ttgcgtagaa
     2221 tcacagaata tttatctgtt cccgtcgaga gaaaagagca tttgacgatc gaaagcgacc
     2281 ctaattgtta tgtagggact tacagaagcg gaatcaatac agtcaatagg atttatcgat
     2341 tagctttaga tgaaaaaaaa cctgtaccaa agcactggca aaagctcaaa gaattagtac
     2401 tgaattcgtc atggcgaaat tttgctttac agatttttga aagtcaggaa catttaaatg
     2461 ttcctgtttc gatgccagaa aaaatggcgg aagagcttta tggaaaagag atttatactt
     2521 caatttcacg tatggagaat ttttataatt gtgaatacaa atacttcatg caatttggtt
     2581 tgaaattgaa agaacgaact gtttatggtc taactccagc agcaactggt gatttttatc
     2641 atgaatcatt ggatcgtttc ttcaagctac tgttttcaaa ccaattatcc ttagtgaata
     2701 tgtccgatgc gcagcgtaga gactttactg aaaaagtgct gcaggaagta ttcggagaac
     2761 ttcggtttga gatattggat agttctgcaa gaatgaacta tatccgctac cagttaggac
     2821 agacgattca aaaagtgaca tgggctttgc aaaaacagag caaaaataca ggcatgcatc
     2881 ctcttcagac agagattcta tttggtcaga ttgctggagc aaaaggtatc acaggtctgg
     2941 aattaccgtt gagtaatggt ggaaaactcc atgttcgagg gaaaatcgat cgaatcgatg
     3001 tagcttctga acaagaagat acttggctaa gcgtcgttga ttacaaatct agcggacgtt
     3061 cattcgatgt cacagaagct tattatggca tggctatgca attattgacg tatctggacg
     3121 tagcattgat ggatgcggta cgtttgactg gaaaggcggc tgttaaacca gcaggatcat
     3181 attatttaca tgttcataat cctgtattga cggagacaga tgatgtagag aagaatacat
     3241 tgaaaaaatt tagttatgat ggcgtttttg tcgatgatcc cgctctttta gaagtactag
     3301 atcattcact ggaagcaaaa gaacaatcac tgatctatcc agtgaaaaaa aatgcgaaag
     3361 atgaatacca aaaaggtagt ttcagtaaag acaaattttt cacggaagaa gaactgcaac
     3421 tattcatggc gcataatcgg gaaaatatgc ggttagctgg agatgcgatt acttccggag
     3481 aaatcaaact aaatccagct tataaagata aacagcggat tgcctgcgaa ttttgtccat
     3541 tcagaagcat ctgtacgttt gatgtgatgc tgaaagaaaa taattaccac cgtctggaga
     3601 aaatagataa ggaagaagca ctgcgtagaa tattgaaaag gagtgaagaa gagtgaatca
     3661 aattccttta aaaccgcaaa atgaacgatt cacagatgat cagtggcaag cgatttttga
     3721 tcaaggagat aatctattag tctctgcgtc tgccggatca ggtaaaacta cagtgcttgt
     3781 tcgaagagtg attgaaaaac taaaaatggg ttttgatatc gatgaattat tgattgtgac
     3841 atttactgaa gctgccgcaa gagaaatgaa agaaagaatt caagaggcat tacaagaatc
     3901 cgtcaatagc gaaagtgacc ctgtacgtag acagcatttt actaagcagt tagttttatt
     3961 gcccacagcg aatatctcaa ccttacatgc tttttgcctg acagttattc gacgttatta
     4021 ttacttaatt gatattgatc cagtctttcg catgttaaca gatgaaacag aaacgatctt
     4081 gatgaaggag gatgtgtggg acgagctgag agaagcactt tatgcagaaa atgatgaacg
     4141 atttttccaa ttaacaatga atttttctaa tgatcgttca gatgacggct tgacgaatct
     4201 ggtgttttct ctttatgagt ttgcccgtgc caaccctgac ccccaaaaat ggctagaaca
     4261 gttaagcgat aattatcgtt tgcctgaggg gctagcgaaa tctcgattat atcaaaaaca
     4321 aatccgtcct ttagttttgg cagatatata ccaatgtgta cagttatatg aacagatgac
     4381 tcagttagca caaggtgaag gattagaaaa aatgcatgag caagtagctg gagaacaaca
     4441 gcagatcaag aatatctatg aagcgttctc gcaagatcgg ttagaagaag cttatgctgg
     4501 tttggaacaa ctaacttttt caacttttaa gagcagtcgg aaagcagaat tgaaagaaat
     4561 ttccaatgaa gtcaagggca tgagggataa agcaaaaaaa ctgattcagc agataagtaa
     4621 aagttatttt cctgtttccc caagccaaat ggaagaatta acggacaaag cactaccgtt
     4681 agtcgaagaa atgacaaaag tcacgcaaag cttcatggac ggtttcagta tgcgtaaacg
     4741 ggaaaaaggt gtgctggatt tcaatgatct ggaacattta gcgttgcaga ttctcacaga
     4801 aaaaacgaaa gatgtatggc tgccaagtga agcttcaaaa cattatcgaa aaaaattcaa
     4861 agaagtaatg gtagatgaat atcaagacgt gaaccaatta caagaagcca ttttatactg
     4921 gctaagagaa cccgatgata caaaaggaaa tatgttcatg gttggggatg tcaaacaatc
     4981 catctattct ttccgtttag cagatcctag tttatttatc gggaaatacg agaatttctc
     5041 aaagaaagaa ggcggacgtc gaatcgtttt ggctgagaac ttccgctcta gaaaagaagt
     5101 cctttctttt acgaatctta tttttgaaca actaatggat ccagcagtgg gtcaaatcaa
     5161 ttatgacgaa gcagccaaac tgatccaagg attttcagac tttccagaga atgaacagtt
     5221 tgagccggaa atcatgatct atgaaaaaga acaggaagaa tcagagattg agattccgac
     5281 ggatgatata ctggaagaca aaacagaagg cgaacttttt atgacaggac taaaaattcg
     5341 tcaattaatt gattcctcct ttatgattta tgataaaaaa tcaaaaaaaa gtcgtccgat
     5401 agagtacaaa gacattgttc tcttgacacc tacaaagaag aacaacttaa cgattttaga
     5461 gattttcaaa acattggata ttccattaga gatgaatgat gctcaaaact attttcaagc
     5521 gacagaaatt cgcacgatga tctcgctgct acaattaatc gataatccct atcaagatat
     5581 tccgcttgcg gctgtattgc gctcaccgat cgtgggtctg attgaaccag aattagccag
     5641 cattcgatta gctaaccgtg cacatactta ctatgatgct gtgcttgctt atcaagcatc
     5701 aaacgaagat gaattagcag ctaagctgga acattttggc aagcaactag agcattggcg
     5761 agaacttgct cgccgttctt cgatcactga cttgttatgg gatatctatt atgagacggg
     5821 atatctagag tatgtggtcg gtctgccagc aggggcacag agacaagcca atctttatgc
     5881 attggttgat cgtgccaaag cttacgagca aagttctttc agaggattat accagttcgt
     5941 gcgtttcatt gaaaaaatgc aagaaaaaga taaagactta gcagaaccag tcatttcaat
     6001 agaagataat gctgttcgcg ttatgacgat ccatgcaagt aaaggcttgg aattcccagt
     6061 tgtatttttg ttagatatga caaaggagtt caacttacaa gacttgcgca atcggtatgc
     6121 ttttgaagaa aaacttggag caggaattcg ctatatggat cctgaaacac gagtgttata
     6181 tgatacgtta ccgtttcaag ccatcaaatt ggcaaagcaa aacaaattgt tgtcggaaga
     6241 aatgcgtaaa ttgtatgtag gattgactcg ggcagaacaa aagcttttca ttgtcggttc
     6301 ctataaaaat aaagagcaaa tgatccaaac gtggtctgaa gcagctgatc atgaggagtt
     6361 agtttttgat cctgcgctac gtttgaaggg cagaagcagc ttgatgaatt ggattggtta
     6421 cggattgatc cgtcatcctg aaatgcagaa atacttagag gaagaaattt cgacttctct
     6481 tcttcaacat tcaaatgcac agttttctat ttcatggatg aatcagcaat cgatcattga
     6541 acaaagacag ttgcttgctg aaaaagaact ggttaactta gatcagcaga tgaaagaaga
     6601 tgaaacttta ttagcagatt cgttacaaaa acgtctggca tatgaatatc cttatcaagc
     6661 ttcttcacaa accacaagtt atcaatctgt atctgaaatc aaacgattat tcgaagatcc
     6721 agatgataca caagaatcac gtttgactct ggaaagcagt cagaacaaag cagcatcccg
     6781 tcaatttcgt tatacgcaag aacaactagc agaaccaaaa tttttgcaaa aagatcggca
     6841 agtgtcagca gctgctgttg gaactgctac gcatgctttg ttacaattac tgccattaga
     6901 gatgccaact acagagagta tccatcaaaa attacaggaa ttggtaagga aacggttagt
     6961 agacgaaaaa gtcgcgaaaa aagtagatgt ttcttctatt atatggttct ttcaaacaga
     7021 acttggtcaa cagctgattg ccaacaaaga aaacgtaaaa cgagaacaac ctttttccat
     7081 gctgcttcct gctgacgaag tatttcaaga ctatccaaat caagaagatg aactattaat
     7141 ccacggaatc gtcgatggat acctagaaga aaaagatcat ctcaatatct atgattttaa
     7201 aacagatttt attttacatc cggatgatcc agcagaaata gatgcaatcg tccaaaaata
     7261 tcaaggacag ctaagacttt accaacaagc aatgtcagaa gctttgaata agccagtaga
     7321 aaatgttttc ctaattttgc ttcgagtgaa acaaattata aacattaaca aataattata
     7381 attgagcaaa cttgacttat gttttgtaag gattatgcta tactctggga agaggtgagt
     7441 atgatggagc taacacagac agaattcgtt ttatgcccta aatttgaaaa aacattctct
     7501 attttaggaa aaaaatggaa cggattgatt atcgatgtac tactcgaaaa cggtccgcaa
     7561 cgctttgttg agcttgcggc aaaaatcccg atggtcagtg atcgagtatt agtcgaacgc
     7621 ttgaaggaat tagaaaaaga aggaatagtc actcgaaact ttgcctgtag cgaaaagagc
     7681 cgttcagaat atctcttgac tgaaaaagga gcagacttgc aaaaagcgat gcgtcaaata
     7741 caagtttgga gcgaaaaatg gatcacagag gaagaatgcg gttgattttg taaaaaaaat
     7801 cttgtaaact atagtccata cgaataagta aagaacgatg acgaaaaaga gtagtttggc
     7861 taaaccagtt cagggagatc tgtcgcagac tgaaagcaga tcctgtgttt gaaaaatgaa
     7921 gttcactttc cgagttcgtc tgataaaatc agaccggcaa ttgtcgttaa cgaagtaaga
     7981 gtgcggtgta agtcatttat ccgcaaatta ggatggtaac gcgaagacct cgttccttaa
     8041 caggaatgag gtctttttgt tttgtatttg gaatttaaag gaggaacaag cacatgtctc
     8101 tacaagcaca attagagagc ttgaaagaag caacgctcgt caaaatcaca gcggcacaag
     8161 atttaaatga attaaatcaa atacgtgttg aaacgttagg aaaaaaagga ccgatcacag
     8221 aagtattacg tgggatgaaa gatctaagtc cagaagagcg accaaaagtc ggaagtttcg
     8281 caaatgagat ccgagatctt ttgacagaag agatcgtgcg aaaaaaacaa gtattagaag
     8341 aagaagcgtt aaatgcagct ttggcaaaag aaacaataga cgtgacacta cctggtagaa
     8401 aagtaaaaca tggtacacgt catatcgtga cacaaatcat ggaagaaatt gaagatattt
     8461 ttgtgggtat gggctatcag atcgtagaag gatatgaagt agaatctgat cattacaatt
     8521 tcgaacggat gaatctgcca aaagatcatc cagcaaggga catgcaagat actttctata
     8581 tttcagacga gatattgatt cggacacata cttcaccagt ccaagcacgg acaatggaaa
     8641 aacatgattt ctctaagggt gctctacgaa tgatctcgcc agggaaagtc ttccgcagag
     8701 atacagatga tgcgacccac agtcatcagt tccatcaaat tgaagggcta gttgttgata
     8761 aaaacatcac aatgggcgac cttaaaggaa cattagaagt cgtaatgaaa aaaatgtttg
     8821 gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc atttacggaa ccatcagtag
     8881 aagtagatgt cagctgtttc aaatgtggtg gtgccggttg taacgtatgt aaatacactg
     8941 gatggatcga aattttagga gctggcatgg tacacccgaa tgtgttgaag atgtcaggaa
     9001 tcgatccaga agaatattca ggctttgctt tcggccttgg accagaccgc gtagctatgt
     9061 tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa tgacttacgt ttcttaagtc
     9121 agttcaaggg ggagtaagaa aatgttagtt tcatataaat ggctcaatcg ttatgtagat
     9181 ttatcgaatg taacaccaaa agaattagcg gataagatgt ctgtgacagg aatcgaagta
     9241 gagggagtaa cggttccaga agaaggcttg aaaaaaatcg ttgtcggtga agtcaaggaa
     9301 tgcgtaccac atccagattc tgatcacctt tctatttgtc aagtcgatat tggcgaagac
     9361 gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg ttggaatcaa agtgattgtt
     9421 gcacttccta actcacgtat cgctggtaat gtaaaaatca aaaaaggaaa aatgcgtggc
     9481 caagtatcaa atggcatgat ttgctcatta caagaaattg gctattcaga tagtgttata
     9541 ccgaaagaat acgctgaagg tatctattat atgccacaag aagctgtaaa tggcgatcca
     9601 gtttttcctt atttggatat ggatgattcg attattgaat tatctatcac accgaatcgt
     9661 gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg gggcaattta tcgtcagact
     9721 cctacattcg atgacgaaaa gctggtagaa tcctcacgtc ctgctagcga taaaattcaa
     9781 gtggaagttg aagataaaga agcagttcct gcttatcaaa tcagaatcat tgaaggagta
     9841 agaatccagc caagtcctca atggctgcaa aatcttttga tgaacgaagg cattcgtcca
     9901 atcaacaatg tagtagatgt gacgaactat attttgcttt tattcggaca accactacat
     9961 gcatttgact acgacaaatt gaaatcaaaa gagatctttg tccgccgtgc aaaagaaaat
    10021 gaaacattag taacactaga tggagaatct cgggaattga caactgacaa tctcgtcatt
    10081 actaacggca aagaaccagt tgctttagct ggtgtcatgg gagggttaga ctcagagatc
    10141 acgaatgaaa caacaacagt tgcactagaa tctgctttat ttgatccaat ttcgattcgt
    10201 cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg cacgttttga aaaaggaatc
    10261 aaccatgcga ctgttggaga agccagtgag gttgctgcag caatgatcgt ttctcttgcg
    10321 ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga tcaaagcaga agacgtatca
    10381 gtgaagattt cacttgaacg catcaatcaa tatttaggaa catctcttgt cgttaaagaa
    10441 gtaaatgaga tttttgaagc acttggattt agctatgaag aaaaggagta tgtttattcc
    10501 gtagtagtac caccaagacg ctgggatatc caaatcgaag cagatattat cgaagaagtt
    10561 gctcgtattt atggatacga ccagttgcca tctacacttc caagtggaga aacggttgct
    10621 ggaagcttgt caaaagaaca agaaactact cgtaaaattc gtacaatctt tgaaggaagc
    10681 ggattgagcg aagcaatcag ttatgcattg acaacagaag aaaaatcaag acagtttgtt
    10741 ataaatgaaa gtaatattac acgtttggac tggccaatga gcgaagaacg ttctgtatta
    10801 cgaatgaatt tgatcagcgg actattagat gatatcagtt ataacactgc ccgaaaaaat
    10861 acagagattg ctttatatga aattggtcgg gtgttcttcc aagaaaatga tccacgtaca
    10921 catctgccgc aagaagtaaa acatgcagca atcgctattt ctggtatttg ggaagaaaaa
    10981 gattggcaga cgaaagctca aaatgtagac tttttcacca tcaagggtct tttagaaaac
    11041 ttatttgaac aactaggcat cactgaagat gtccattatc aaacagtgac agaaatgaag
    11101 gaaatgcatc ctggaagaac tgctgcgatt taccttggcg aaaaatttat tggttttgtc
    11161 ggtcaagtgc atccaacgat tacaaaaagt tatgatattc cagaaacata tgttgctgag
    11221 tttgatttat ctgctatagt ggaagctgct caaaaaggga tcgttttcga agcagtgaca
    11281 aaattcccag cagtcagccg ggacatcgct ttgcttgtaa aagaaactgt gacaaatcag
    11341 gaattaacag aagttatccg ttctgctgcc ggaagatttt taacagatat ccaattattt
    11401 gatgtctatc aaggagaaaa cattgaaaaa ggacataaat ccatggcgta tagcctgaca
    11461 ttcgtcaacc cagaagcaac attgacagat gaagaaatca ataaagcaat ggaaaaagtc
    11521 gaaaaagcgt tggtagaaaa cctagaggca agtattcgct aagaaggaaa atatagtaag
    11581 taacgatcaa gtagcttttg aagaccatac atttgaaaaa aagatgtgac acaacttcgt
    11641 cacatctctt tttttgattt taaaaaagga atagcacaga gacgagataa aaaaacaggc
    11701 ctttacagcc tgctttttta gagtaaatga attaatggaa gtaattgatt cccattgctg
    11761 attttacttc agaaagtgtt tgtgcagcta ctttagcagc ttcttcacta cctttacgta
    11821 agatttccat aacagctgaa ggatcttttt ccaattcttc tcggcgcgca cggatcggtg
    11881 caaattcagc ttccaataca tcaatgaggt atcgtttgat ctttacatca ccaagtccgc
    11941 cgcggcggta atgagctttc atttcttcaa ttgcttcttt atctgttccg aatacatcca
    12001 agtaggtgaa gaccatattc ccttctactt gtccaggatc ttgtacatgg atgtggtttg
    12061 gatcagtata catactcatg acttttttct gtaagacatc ggcaggatcg gagatataaa
    12121 taccgtttcc taacgattta ctcattttgc catttccatc gattccaggt aatctaccca
    12181 ttccttttgg tgggaatacg ccttttggtt ccaccaagac ttcaccatat gtatgattga
    12241 agctttgtac gatttcctgt gtctgctcta gcattggttt ttgatcctca ccaacaggta
    12301 caagattcgc tttgaatgca gtgatatctg ctgcttgaga aacaggatag ataaagaatc
    12361 ctgtaggcac gctttcaccg aatttttttt gttcgatttc tgttttgaca gtcggattgc
    12421 gccgaacgcg tcctacgctg actaagttca aataatacat ggttaattca gctaattccg
    12481 gaatttgtga ttggataaat aaagtagatt ttgcaggatc taatcccacg gctaaatagt
    12541 ctaaagcaac ttgcaagaca tttgatgaaa ccttctcagg atttttagca ttatctgtca
    12601 gtgcttgcat gtcagcaatc atgacaaaca gttggttgtc ttcatctgcc tgcatagcca
    12661 ctcgggtttt tagagaaccg acgtaatgcc ctaaatgcag cttcccagtt ggtcgatctc
    12721 ccgttaaaat aatatttttc aaaaaagaca cctctttacg atttgtaagt ctgagcactt
    12781 aatatcttac cgtttttcct gaaaagaaac aacagttcct aaggaaaaaa caaggaaaga
    12841 ggataaaata actaatagat tagaaatgga agaaatattt atgggagaat aaacgttcta
    12901 ag
//
LOCUS       DACCWV010000081        12791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCWV010000081 DACCWV010000000
VERSION     DACCWV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.081"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(95..1624)
                     /gene="ahpF"
                     /locus_tag="IVW84_001889"
     CDS             complement(95..1624)
                     /gene="ahpF"
                     /locus_tag="IVW84_001889"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7918076.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1634..2197)
                     /gene="ahpC"
                     /locus_tag="IVW84_001890"
     CDS             complement(1634..2197)
                     /gene="ahpC"
                     /locus_tag="IVW84_001890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7918077.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2341..3003)
                     /locus_tag="IVW84_001891"
     CDS             complement(2341..3003)
                     /locus_tag="IVW84_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7918078.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3187..4833
                     /locus_tag="IVW84_001892"
     CDS             3187..4833
                     /locus_tag="IVW84_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7918079.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4824..5906
                     /gene="uxuA"
                     /locus_tag="IVW84_001893"
     CDS             4824..5906
                     /gene="uxuA"
                     /locus_tag="IVW84_001893"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7918080.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(6014..7567)
                     /locus_tag="IVW84_001894"
     CDS             complement(6014..7567)
                     /locus_tag="IVW84_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7918081.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            7715..9003
                     /locus_tag="IVW84_001895"
                     /pseudo
     CDS             7715..9003
                     /locus_tag="IVW84_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(9129..10208)
                     /locus_tag="IVW84_001896"
     CDS             complement(9129..10208)
                     /locus_tag="IVW84_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7918082.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10311..10751)
                     /locus_tag="IVW84_001897"
     CDS             complement(10311..10751)
                     /locus_tag="IVW84_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7918083.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10808..11218)
                     /locus_tag="IVW84_001898"
     CDS             complement(10808..11218)
                     /locus_tag="IVW84_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7918084.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11398..11832)
                     /locus_tag="IVW84_001899"
     CDS             complement(11398..11832)
                     /locus_tag="IVW84_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7918085.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11932..12780)
                     /locus_tag="IVW84_001900"
     CDS             complement(11932..12780)
                     /locus_tag="IVW84_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7918086.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4304 a         2382 c         2277 g         3828 t
ORIGIN      
        1 aaaaagcgat tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt
       61 tttccgctct ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg
      121 ctcctaaagc tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat
      181 ctgtacaatc accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca
      241 ctatttcatt tcttggattc atttcgatcg ttccatctag ccaatccgta ttagggagta
      301 agccgactag aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat
      361 cgatatagga gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt
      421 tgataacagt gacattcgca agagagacta gtttatcctg taagatttga tctgctttta
      481 attctggtaa gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg
      541 cttcaacacc agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc
      601 cgtcacagtg tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat
      661 tgatagaacg ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac
      721 cgttagctaa gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat
      781 tcatcataat gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg
      841 gaccttctgt gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc
      901 cgccgaaaga atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg
      961 cactactagc tgctggtcca ccgccaatta ctaatacatc ataaggttct ttttcaaaca
     1021 actcttcttc aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc
     1081 cgctactaaa ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat
     1141 cgatttcttt ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata
     1201 cagctaatat attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat
     1261 aactgtcaaa atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga
     1321 ttttcggtgc ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac
     1381 cgagggggat accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac
     1441 taggtgtgcg catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt
     1501 ccttcgtaaa ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa
     1561 caataggaga ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta
     1621 acatatccac cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac
     1681 tttctttcca ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc
     1741 gaatcttatc aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag
     1801 cttgaacgat tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt
     1861 catccaaaac atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt
     1921 cgattttacc gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg
     1981 tagagcaaga aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt
     2041 cttctaattc agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc
     2101 agttccccat aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg
     2161 cattaaattc ttcgattttt ttcccaatca atgacatagt aaaacctcca ataataatta
     2221 tttatttata attattgtaa tttagttgga gttgcaaagt caatggatat tttgttattt
     2281 aataaagatt actcaaaaac gaaggttaat tataaaaata gaagaggtcg caagaaaatg
     2341 ctactgctgg aaatactcgg gataacgttg agtaacagtt tcctgttctt tcagcatcat
     2401 gtgtaagtgc aatcccgttt gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt
     2461 gagtaaatca taatgttgtt ccatgatcgt ttcccatgga agcccctgaa tggtcactcg
     2521 taaccagcgg aaacgttcta aatgggtatt atggtcatct aaccagttcc aaacttcttt
     2581 tcttcctgca atttcgaaac aagcttcatg aaataaatta tctgcacgga aaaaagcgcg
     2641 ttcgtcattt attttgactg ctttttcttg ttcttctaaa atcgtttcta gcactattcg
     2701 tgtttgtgtg tccaatttgg aacaacattc aatcatgatt tgctcttcca actgttctcg
     2761 gacaaagcga gcattttctg cagaaacaag attaattttg gaaacatacg taccactttg
     2821 aggtatcgta tacacgagtt cttgctgacg tagttgaatc aatgcttcgc gaatgggagt
     2881 ccggccgata gcaagatgtt cctccagtgc cttttcagat attttccttc caggttctaa
     2941 gtcagcatag ataatttgtt tacgaatcgc ttgataagct tgtgattgca agctgctttt
     3001 catctttatt cctccaaaat catttctatg aacgtagtat aacagaagag gtctgaaaaa
     3061 caatctggta tgtcacatta actagtaaat ttgtataaat agtaaaattt tggttgacat
     3121 agcgattttt attgtataaa attcacttga atacaaaaac taatatgtta gtttaggagg
     3181 ggaaaaatga ttgatttaaa caactggagg aactttatcc ctgaattatc tcaaaaagaa
     3241 gtagaaatac ctcattttga ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc
     3301 tggatccatt tcggtggcgg aaatctttat cgaggatttc atgcagaaat cgctcaacgt
     3361 ttagcggatc taggcgaatt gtccactggt gtcattgttt gtgaaacatt cgatgaagaa
     3421 ataattgaaa gggcttacca agattttgat gatttgattc ttgaagtggt catgccaagc
     3481 agtggagagt tcgtcaaacg tatattagct gcgacagctg acagtctgta ttgtcatcca
     3541 gattgttctg aacactttga taagatgatt cgacactttg aaaatcctag tttacaatta
     3601 gttacttttg ctattactga aaaaggttat cgattgaaag atacaaataa taaatatata
     3661 ggaccgattg cagtcgatat aaaaaatggt ccaaagaagc catcacatac aatgagtatt
     3721 cttgctgcgc tactttataa acgtttttta tctggagctt ggccgattgc acttgtctct
     3781 acagataatt tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa
     3841 gaatggcaaa aggaaggtta tgtacaaaat acatttatcc aatacttatc gaatgaagaa
     3901 attgttgcgt ttccatggac gatgattgat cgtatcaccc cgaatccttc cttgtcagtt
     3961 agcaaaatgt tggcaaaaga gggcttttca aatatgtcga ttttccaaac cgcaaaagga
     4021 aataatattg ctccatttgt caatacagag gacatccatt atttaatcat ggaagatcat
     4081 tttcccaatg gacgtccgaa attcgaaaaa gctggggtat tcttgactga tcgtgcaacg
     4141 gtagataaag cagatacgat gaaggtgacg acttgcctta atcctttgca tacgtcttta
     4201 gctattatgg gttgtttact ggggtatagg agcatagctg aggagatgaa agatccagat
     4261 ttatcgctgc tagtttctga aatcgggtac aatgaaggcc tcccagttgt ggagtctcca
     4321 ggtatcattg atccaaagat ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca
     4381 aatattcctg atatgcctca gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt
     4441 tatggtgaga cgattaaaaa atatatggaa atagaagtta aagacccgca gacacttgta
     4501 ttcataccat tgactctcgc tgcttggctt cgatacctta ttggcattga tgatcaagga
     4561 aaaccattca tcccgagtcc agacccacta ttgacagact ttcagcataa gctttccgat
     4621 attcaactgg gtacgcaaga tacatcacat attcatgaga aaatggctcc tattttgtca
     4681 aataaagaaa tttttggtgt agatctttac caagcagagc ttggcagaaa aatagaagaa
     4741 atgttcatca agatggtagc aggaccgaat gccgtacgaa aaacattaca ggattactta
     4801 caagaacatg gaggaaattt atcatggaaa tgacttttag atggtacgga tcacaggaag
     4861 aaaagattac cttagatgaa atcagacaga ttcctgcggt caaagggatt gtaggcacat
     4921 tgatggatat accagtagga gaggtatggc ccagggagag gatcaaagaa ctaaaagaag
     4981 aaattgagtc tcgtgggtta acactaaaag taattgaaag cgtcaatgtg catgatgcca
     5041 ttaaaattgg attaccttcc cgtgagcagt atatcgaaaa ctataagcag acaatccgaa
     5101 acttggcaga atatggaata gaagtcatct gctataattt tatgcctgta tttgactggg
     5161 tgaaatctga ccttgactac cgcttagaag acggctcatc cacgttagct tttatatctg
     5221 atgatattcc agatgatcca agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat
     5281 ttgaattgcc gggatgggaa cctgagcgct tagctcatat caaatccttg cttgaagcct
     5341 atgcatcagt agacgaagaa aaacttcgag aaaattttgc ctattttctc caatcaatca
     5401 tcccaacatg tgaagaggta ggggtcaaaa tggctgtaca tccagatgat ccgccatact
     5461 cgatttttgg attacctcga atcgtaaaga atcgagaaga tttagattgg ttgtgccgca
     5521 tagtagacag cccttataac ggaatcactc tttgtacagg gagtattgca gaagatccag
     5581 aaaatgatgt atatgggatt ttagcagaat tttgtcaaag agatcgaatc ccatttgcac
     5641 atgttcgaaa tattaaattt ttagctaatc gagatttcta tgaggcaccg cataaatccg
     5701 aatacggttc attagacatg taccgcatac taaaaacaat gtatgacaac ggatttaacg
     5761 aatacatccg tcctgatcat ggaagaatga tctggaatga gacaggaaga ccaggatacg
     5821 gcttatatga tcgagcatta ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa
     5881 aaggaaacaa aagtaaagat aattagtgaa aaaaactgta attttattat gtaaataaga
     5941 aggatgttca aattcttgat acataaatcc ggaggttgtt aataagcatt cggatttatg
     6001 tatcttattt ggtctaggaa gtaacttgat tttctttttt cgattgatcc atgagtgctt
     6061 cttcttttaa ttcaaagata tattctgaga tcctcataat cacttttttc cagtcttctg
     6121 gtgtgatgat tccttttaaa atatagattt ttttgatatg agaaggcaat tgtttcaaag
     6181 ggcagtcgct gataacgatg ttgcagttct ccataaaatc attttgtatt ttgatcgatt
     6241 catttgaaaa aatttgttct atcttaacga tcagatcttt actgatataa aaatcacgcg
     6301 tatagttcac accaatcaca attcgcggct gttttttgta gcgatcatac aaatgtacta
     6361 ggtcttcaac catccattgc acattttcct tttctaaaat aactggtaac aagtgttgat
     6421 tttgttctct gaaatccata taaaattgtt caatggcggt ttggacttct ttcaacacgc
     6481 tatgttcttg ataaagcatg aaagtaggaa ggggcgtatt tggttgattg aagttaagaa
     6541 atccattata aagtttattt cgtgcgaatg ccaatgagta aagaatcttt tctttttcag
     6601 gtataaacaa atcaaataca gtacaaaagg actccaccat ttctcttgaa taaagaacat
     6661 gaggaacgtt tgcttttttt aaaagtttgt attgctccaa gcttgtttct tccgattcga
     6721 ttttcgtgat agaaagtcga gctaaaatat tcactaagat gcgttgttcg cgacaaattg
     6781 gcagattagg tcgcaaaata tctacagagg gatcaataaa aaagtcaata tattcatact
     6841 ctgtgaattc tttttgtacg tctttcacag aagttgcagg aaaattctct ttacatattt
     6901 ttaatagaaa atataattta tctatcatac cgctatttag tcggtcaagt acttcttttt
     6961 taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa aattctgtag
     7021 cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt tgtacctctt
     7081 ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg tacaatgcta
     7141 aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag tttgtttgct
     7201 gacttaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag atctgataac
     7261 tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc ggatcaattc
     7321 cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct gttagattga
     7381 gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat tcagacatat
     7441 ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa
     7501 aaatatctaa ttttgtattg ctttttttct tcaagagaaa cgttttaatc gcttcttgag
     7561 tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata ttactgtttt
     7621 tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac tatcttattt
     7681 cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca tcttaatatc
     7741 aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca cagaggagat
     7801 agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga
     7861 tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat
     7921 caaagcaaaa attgactatt caggagactt agcacctata ctacaatcag ctgttacatt
     7981 tgagaatgtt gatcccgctt tgtcgattgg ctatgacgag aacactttga ctatccaaca
     8041 caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct
     8101 ttttactgtc aaggtatcag atggactacc gaacgatcat catcatttaa cgccgtatag
     8161 tcagcggcag acaattgagc aagattcagc tatcgactca ggaggagatc ttgttgaaga
     8221 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
     8281 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
     8341 gccagacaat gaatcaaaac ctgaaataac ccctggagaa aaaccagatg tagacccaga
     8401 agaaaaacca gatgtgactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga
     8461 aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg tagccccaga
     8521 agaaaaacca gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga
     8581 aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga
     8641 agaaaaacca gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga
     8701 aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct
     8761 agcaattcta aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc
     8821 ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa
     8881 aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc
     8941 attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc
     9001 taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg
     9061 accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca
     9121 tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc
     9181 aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag
     9241 aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg
     9301 gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc
     9361 aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata
     9421 caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa
     9481 ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa
     9541 agagcgacaa caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca
     9601 aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat
     9661 acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac
     9721 gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga
     9781 agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct
     9841 gcatcttttg gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga
     9901 tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta
     9961 taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca
    10021 agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta
    10081 actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc
    10141 gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg
    10201 ttattcatta tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg
    10261 acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt
    10321 ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat
    10381 tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt
    10441 tgcaacgcag caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca
    10501 cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca
    10561 gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa
    10621 actaacatca caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag
    10681 tgtctaatga tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat
    10741 agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca
    10801 aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct gtagcttttg
    10861 aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac
    10921 cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa
    10981 gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt
    11041 ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg
    11101 cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat
    11161 gcacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa
    11221 aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag
    11281 caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct
    11341 cgaatagcaa acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta
    11401 catatatttc tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg
    11461 aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat
    11521 catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc
    11581 ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc
    11641 agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa
    11701 aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa
    11761 gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt
    11821 tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt
    11881 ataccagcta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat
    11941 tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc
    12001 acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac
    12061 gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag
    12121 gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat
    12181 gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt
    12241 gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag
    12301 agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt
    12361 tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat
    12421 cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt
    12481 aatcattgtt tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac
    12541 tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa
    12601 cactaaaata actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc
    12661 gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa
    12721 tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat
    12781 ttattcactt c
//
LOCUS       DACCWV010000082        12790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCWV010000082 DACCWV010000000
VERSION     DACCWV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.082"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            103..1146
                     /locus_tag="IVW84_001901"
     CDS             103..1146
                     /locus_tag="IVW84_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP7918087.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1319..1543
                     /locus_tag="IVW84_001902"
     CDS             1319..1543
                     /locus_tag="IVW84_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918088.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1546..1803
                     /locus_tag="IVW84_001903"
     CDS             1546..1803
                     /locus_tag="IVW84_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918089.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2020..2895
                     /locus_tag="IVW84_001904"
     CDS             2020..2895
                     /locus_tag="IVW84_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918090.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2905..3699
                     /locus_tag="IVW84_001905"
     CDS             2905..3699
                     /locus_tag="IVW84_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918091.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3728..4846
                     /locus_tag="IVW84_001906"
     CDS             3728..4846
                     /locus_tag="IVW84_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918092.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4879..5199
                     /locus_tag="IVW84_001907"
     CDS             4879..5199
                     /locus_tag="IVW84_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918093.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5454..6143
                     /locus_tag="IVW84_001908"
     CDS             5454..6143
                     /locus_tag="IVW84_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP7918094.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6144..6347
                     /locus_tag="IVW84_001909"
     CDS             6144..6347
                     /locus_tag="IVW84_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918095.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6367..7995
                     /locus_tag="IVW84_001910"
     CDS             6367..7995
                     /locus_tag="IVW84_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918096.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8046..8417
                     /locus_tag="IVW84_001911"
     CDS             8046..8417
                     /locus_tag="IVW84_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918097.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8502..8702
                     /locus_tag="IVW84_001912"
     CDS             8502..8702
                     /locus_tag="IVW84_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918098.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8836..10062
                     /locus_tag="IVW84_001913"
     CDS             8836..10062
                     /locus_tag="IVW84_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918099.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10912..12237
                     /locus_tag="IVW84_001914"
     CDS             10912..12237
                     /locus_tag="IVW84_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7918100.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            12230..12577
                     /locus_tag="IVW84_001915"
     CDS             12230..12577
                     /locus_tag="IVW84_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918101.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         4968 a         1587 c         2549 g         3686 t
ORIGIN      
        1 aacaacgatt atgatcttaa aacaatcgtt tatgtaattg gcaatcttac gaataatttt
       61 catgcaataa tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa atttttagta
      121 ggaattctat tcacaatggg gctagtatta ttatcacctt cagtttcagc agaaagctct
      181 atttccatgt atcgtatgta taatcccaat agcggagagc atctatacac tagaagcgtt
      241 ggagaaagag acaatctgaa acgttatgga tggcattacg aaggtgtcgc atggcaagct
      301 ccaacaagtg gtactcctgt ttatcgtttg tataatcggt ataacggtga acatttttac
      361 acagttgatg ccggagaaag agataagatt tcacacacag gatggacgta cgaaggtgtt
      421 gcgttctatt cctacaacgg agcaaataga gttcctgtaa cacgtttata taacaaatac
      481 gtgaattggc atcactacac gttagatcaa aatgaaaaga acgtaattag taaacaaggt
      541 tggaagattg aaggtacagg ctggtatgca atgccaaaca gcgtaccaaa tcctacacct
      601 gctccaagca ctgaaaaagt gttgaacgtt cctgttgtat ggcaaggaaa tacaatgtta
      661 tgcgaaggag cttctttatt agaaggttta cactacaaag gtgtagccac aaatcaaagc
      721 ttaaccagtt ttgtaaacta tatgccaaaa gctaatgaca ataatcctta tcatggttac
      781 tcaggagagt ggcgtcataa cgttaatgga acgtatcaag gcatgatggc tgacccagta
      841 gttaaatggg caaaaaacgt tggcggtaat gccgtcaata tcactggttc aggaacgaat
      901 ggaatcaaga atgaaattcg caatggtaat ccggttgtaa cttggattac ttataactat
      961 gctacgccag agttcaaaca aatgccttgg ggtcgtgcag tatggaatgg acatgtagtt
     1021 ctcgtggatg gcttcagaaa tggtgcatac catgttgttg accctgtatt tggcgcaaaa
     1081 tggatcaatg caagcacgta tgagagagca tttaatgtga ctggaatggc ggttgctgta
     1141 cgttaattga tagaattatc aactaaatga aataagagct taattactga aatggtaaat
     1201 aggctcttat ttttttagag gaaatttttg taatacgaat ctataacaaa aaatattgaa
     1261 tgtagtacac aaaagtggta tattaaaatc gtagagatta tgcgaagagg tgattttgat
     1321 gaaccaaaga agaagaaaaa ggttagaaga aataagcgag cgactttccg agctaaagga
     1381 tgagttagaa gtaattcaag aagaagagta caaagaaaat atacctaaaa atctacaggg
     1441 atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa cttgaagaag ctgttgatgg
     1501 tttatccgac atattagaat tgattgaaga tatagctagc tagagatgat taatgaagat
     1561 ttcaggagtg agaataaaat gattgatatt tcactgatag agaagctact tttttcaaat
     1621 aagtacacag ccaaagagtt ggctacaaga tggcaaatat ccgaatcagc tattaaagcc
     1681 tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga aaggaatcag cattggaaaa
     1741 gcagaagaca taatgaaacg tgaaggattt caagctgaca atgattctaa aaaagttgaa
     1801 tagtttaata gtcgccaaga aaagtaccag gacgagggga aaggtagatt acaaaggaaa
     1861 acacttttaa ataaacttcg caaaagcatc aggttatgag tggctagggc tatacgaatg
     1921 tgatgaaaac aaagggcttt aatgtcaaag aaaacgcaat agggggggaa caaaattttt
     1981 aaaaaagata tttatgtttg gtttaatgga aaaaaatata tgactactaa caaaaggtgg
     2041 tcagagctag tacaaaatac agttaaaaaa gtcatgccag atggatatca tttcaactat
     2101 atacaatgct cacaaacaac tgaaagtatt tatttttcaa tattaaatga gaatagactc
     2161 tattatttca gggtaagttc acaccatagg aaaaagagag aagataatgt tacgacattt
     2221 ttatcgggaa aatacaatgg cttcaaagag ttaagtgaag ccattggtga gtatctaaaa
     2281 agtaatagat ttgtcagaat ttctgacaaa catatattga tcttgttact ttatttgaat
     2341 aatagcttca aattgttttg gagagacgac actttctact atattgagaa cggcatggat
     2401 atttattctg gtatcaacgg catattaaaa cattatatta aggcgttact tcgatatggg
     2461 gttctttatc aagaagattt aagtaaccaa attaagatta gtaattctgg aaagtcatta
     2521 ctggcaaaat ataaaaaatt gctttctcct atctacaaag gaatagatat ggaagctgta
     2581 aattctgaaa ctttatttaa tttagttgtt gatgaaaagt ttgcgaatga attttttaaa
     2641 agtaaaaaaa ttgaaagact aaataacctg tttcctgaaa atgcaataaa agcttatgta
     2701 gataataata aatcacgatt atacaactgg tttattccca aacggttgcg aggaaaaatc
     2761 aaaattggcg atcaagtgaa agtaattaat ctaaatcata ctaagatcgt taccgtaacg
     2821 gagttgtata tagctgaaaa agaagatatt acaaaaatga aaccagttat taaaatacta
     2881 gataacaaag agtgagtgga atatatggta gcaaaaaaga atactctaaa agaagcttct
     2941 gaaaaatatc aagatatgaa aagacgagga ttaataccaa aggacaaaaa aggtatatgg
     3001 agagaatttg ttaaggaagt cagtagtgat cgaagtggcg gtgactttgt tggacgatat
     3061 acagcaaaag gttatagcag gcgcttaagg atcactgttg aatatggaac gaatgaatca
     3121 gctacagagc tgaagcaaag gtacttagat acacgaagag aagcttcaaa aaaatatgat
     3181 gatttcaaga gcagaggtct aattcctaag aagaaaaagg ggatttggcg tgagtttttc
     3241 aaagaagaag atagcaatcg gaataaggga gacttcattg gacgatacac agcaaaaggc
     3301 tacactaaga atttagggat caaggttgaa tacggaactg gtgaaagttc atgggaattg
     3361 agaaatcgct atttaaatac attgttagag aaatataaag cagaattatt ggagttgttc
     3421 gatgttaatg attttatcga gatggctgaa aagtatggtc atgaagaagg ttttatgatc
     3481 aatgacatta tggttattga taattttacg gatttattag cacttgcgaa aaaagcaagg
     3541 gatttaagag aaacgcagat gtatctattc agaaataaaa acggaagaaa ccggagaact
     3601 gaatcgagtg agattgaaga ttactatgat gcctttacac acgatctggg agcctttgta
     3661 gacgagataa gtggtgtaat gaacaaatat agggtctaga ataaaaaagc cggagatgag
     3721 cagataaatg ggaaacaata gcatatacac acctgaaatg cagaataaaa ttaaaagact
     3781 tgtcggatta gggtggatga caagaggaaa attagattct tttagtacga tcaaatatct
     3841 tttgatggac atttaccgtc agaacaagca aacaggaata cattttttgg gctatgataa
     3901 gccggaagat gcacctttct ttgttccaag ttcagcctat agagccagta cctccggtag
     3961 catgtataac ttggatttgt ttgaggaagg agcaaagatg atcgaatatg atttcaacga
     4021 agcctattcg aggatcatga gaaattatcc tttgccgtca aacacttatt tgaaagcaaa
     4081 atataataca gatcaagcta ttttgagagc gataaataga ccaaaagtaa gaccgtatga
     4141 aaatttaaaa acgtatatgt tctttttggt taattttgaa ggaacagcca aagaaggaac
     4201 atatgctaat ccaaaatcac agtcgatgtt tgcggattat ggcgaaaatc ccaaaacagc
     4261 taatgagcct atttggttat cggagattga gctaacactt atccttgatt attatgatat
     4321 taaagagatg gaagtcctag atagccacac atttttgacg aaaaaaggat tactcaaaga
     4381 atattttgaa agaatcgagc cattaaaaac tgatcctgat acgttgaagc tttataaaaa
     4441 tcttagaacg aaagtatttg gtgctatcgg acaaaaatca ttaagagaag gagaaacaag
     4501 caccttcgat aaatacagta tgtataatcg agcgtattcc tccatggtag caggcgtatt
     4561 tagagatcgt atggttagat atgaacagaa atacgttcat agcgcatatg ggctattaaa
     4621 aattcgtact gatggattgt atttcagatc cgaagtacca gagtttgaag agctgtataa
     4681 acttggagta gttaagaaaa aagttcatac aatatccaaa gaagaagtcg aaaaagcttt
     4741 agaatttaaa aacaaaggat ttgaaaataa gggctccttt gggaaatcta aagatgaagt
     4801 taagggtgtt gtgatagacg atgatttcga gtttgatttt gactaaatac tctttggaaa
     4861 agatggtagg aggtaaatat gaagaagaaa attgtggaag gaaatgtagg tgttattatt
     4921 ctaaaagatt caactgacta tgctaaatca atggcactaa aagacgaaaa agttgcacgc
     4981 atggataaag atttagttga atttctgatg gatgatacag aaagattaaa aggggatcag
     5041 tttttgagtt taatgaaaga caaaggctta aacacatatg gtttatttga ttataagaaa
     5101 ttagctatag agtgggtaaa agaaggagaa atatttttta ttgaagaatg ggaaggtact
     5161 gagaacttag ttcttcatca gtatcttttg aaagcttaat ctaagtaaaa cagttctgac
     5221 tagttatgct tgtcagaatt tcaaaggaag ttttccataa ccatcatcat tcaaaaataa
     5281 aggatttgaa aataagggtt cctttgggaa atctaaagat gaagttaagg gtgttgtgat
     5341 agacgatgat ttcaagtttg attttgacta gatttttttg caatgtttaa gagtaaacaa
     5401 ggtaagaact aagatcatga agattgagag taaagaagat agaggacgaa tgtatgagaa
     5461 cacactatat ttatttagaa gataaaattg tagatgaaat tccggcaaaa agtcgagaac
     5521 aagctagaaa attatttatt gaaaaataca aagagatcaa tggagatagt gaagttccaa
     5581 aagtggtcgt aacagttaga aaaaggcaaa caaaaaagag aagtacgttt catgtcagcc
     5641 ctgatatata taatcttata gagcgacata gaagtgaaga taatatttct atctctcaat
     5701 atattgctac agttattgat gaattgattt caggaaagta tgactatgct tttgattttg
     5761 agggtgaaaa ggattacaaa agtgttgctg tctttcaatc tgattttgag aagctaaaaa
     5821 actatgctgg caggagaaat gttaattttg caagactact agaagagttc tactccgaag
     5881 agttgggagt aggaaatgaa tacaagagag aaacttatgc gaaattgtct cataagttat
     5941 cccaagcgct taaagaagtg acagaagtta aagcggaact agatgctaaa cttgaagaag
     6001 tgaaaaaata tcaaacagag aggaagaaca acatgaagaa tcaagtggaa tttgacgaac
     6061 agaaagaatt tgaaaaagca agaaaacgct ataacaaaat tatgaatgaa cttagctatg
     6121 aacacttaac agttcatgaa taattgagtg agggtacaga aaaatatacg atcgcagata
     6181 tggttaatga agtggaaact cttttagctt ctttctttga aaatggtcac gtaaacgatg
     6241 aatggcgaaa agaagaacct aaaagggtta acgcttatat tgctcgatta aaaaggttta
     6301 ttgataaata taaggagaat agtgacaaat tttctaaaga taaataagga gagagtgtga
     6361 ttacatttgc cagaaatcga aggaatattt tcgccaaaaa taggtaaaaa acagttagat
     6421 ttcagcagtt ttccgagcgt aaatagagat tataatctag gtgatgaggt acagatacta
     6481 aatattgatt tcaaagaact agacttcgct gttgctgttt ccaacaaacc caaggcattg
     6541 gaacgagtga aaaaaatata ccaagagaat gatcaaaata tcaaaaatat gatcaaaaag
     6601 tatagacgag ataattcaaa agttgaagac gttttattca acggtacggc aaaacatgat
     6661 ttgaatgttt atatggcatt aggagtggct ttgtactgta attcaattaa tcagacagat
     6721 attttggtta atatcgtgag gaatagctat caaaaaatat taaaaataag tagcgaaaca
     6781 aacccttatt atgcaatgaa atatcttggg gataatttat taaatgataa aaaattgatg
     6841 cacaacactg aagagctgtt atctaatttg gctgcgatta actttctatt caaaacaggt
     6901 tctgaagatt attttataga taacgtacta aaaggtgctt acggaacagc attttatccg
     6961 ctttacaata tgaaacagca taaagccgaa caaaaggata acaaacttga aattaaaaag
     7021 atgaaagaac tattaggtat aggatttgta aaacccaatt caaaagtcac tattgcttcg
     7081 ttagcaacta ctaacaatga tgggaaaata gtaaatgggt ttgacgattc agatatagat
     7141 atattgtcaa aatcatattt ttctttgtat ggagattttg cagatttgat gggtattgat
     7201 agttacttag tcaataattt tgagatcaag ggtagcgagc tgaatgatat tgtgtatttc
     7261 gttttaatct ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg
     7321 ctagtttttt atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag
     7381 gaagaattga tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa
     7441 gaaaacaaat ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac
     7501 ggtcggttag aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag
     7561 attaaagaat taaaaggaat cagaaaagaa cttgaacagc tgaggtttga aaataaagta
     7621 ttaaagcaat tgcaggagca agaacataga caatcattaa atggtaaaac tgaagagcct
     7681 tcactgtctg aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat
     7741 gtaaattttc aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc
     7801 gaaaataatg gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca
     7861 gattacttga atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag
     7921 atattttatt tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt
     7981 ttatcactta tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag
     8041 tcactatgag agaactaact ttatcttcta aggaattaga aaagaaaact gctttattaa
     8101 acgaaatatt tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac
     8161 taacagatca ggaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg
     8221 atgaatcagc aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg
     8281 gaaaaatgaa aagatttaat gtgatattag aaatttttgc ttggttaatc ccttcattta
     8341 tttggttttt ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttggac
     8401 tgttaattcc gatataaata ggttcagcaa tttactttaa taacttgtat acacgtattc
     8461 aaataaaaaa gcagttatta catgaaggat taagtggtaa tatgttaaaa agaattgcat
     8521 gggataaaga taataaaaca gatattgagc ttaaagaata taaatacaat tatttctcga
     8581 gatgtataga tagaaagtta ctgtttaaaa aagaaggatt agaatttagt tttgatgaag
     8641 atgaatttga aaaattagtg atggactggc gtaagaattt actggatacg gtacaattct
     8701 aaaagcaatt gaggtcgaaa ttccgatact tgaatcatga tctatattat cgtgttatga
     8761 gtgaaatttc taataaagat aagatatttc atttggacag tcatgtgaat atgagcttaa
     8821 caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt gctaaaattg
     8881 acaacaataa ctatgacaaa aaaaatagaa gtatatgttg atggatcaca agaagaaagg
     8941 gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc gttgaatctg
     9001 gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga aatggatcaa
     9061 ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc atatactgat
     9121 ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt gagaaatgaa
     9181 aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag tcaaacgaga
     9241 acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga ttccgcaaat
     9301 atgaaagcta gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg acaaagaacg
     9361 cctagaatat ataaaaatag aaacaaatat ggattactac cttttagagg tcgagatatt
     9421 aaaatccata aagatgataa gttttatatt tatttaccta aaggctttag gtttaaagtg
     9481 aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca acacattatg
     9541 gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa attatttttt
     9601 aatctaacaa tttcttttga aaaaagctat aatgaaatgg aattcaagaa aggtaatgaa
     9661 ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt taattaatga taaatctgta
     9721 atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt agcaatgaaa
     9781 tcaaaagaac aaagccgagg agtcttttct aagggcggtc atggtcgaaa aagaaaatta
     9841 gatcaagatc gatgggaagc aataaagaaa agagaaagta attttgttaa aacttataat
     9901 aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca aattattcgt
     9961 atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa agaacaacta
    10021 gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt agtaaattaa acaatcgttg
    10081 atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagttg
    10141 tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa acagcatgtt
    10201 tctcaaattt ttaggataag ggaaattgct agcagtttgt tttagaacca tataaaacag
    10261 catgtttctc aaatgagtac tttaaattaa ttgatggcgt tcctgcttta gaaccatata
    10321 aaacaacatg gatttaaaac aagaagtaag tcagttctcc ttttacttta gtaaggaaaa
    10381 ataaagtatt ctcaataaaa cttaaaataa gcaaaccttt gttttaatag ctaatcgaag
    10441 taaaacccaa agccatatga acccctgaat cagcgaaagt ttatgatgat ttggattact
    10501 aatagtttag gaattagatg gtaaatctaa attaaacaat cgttgatttg gcaacgaaat
    10561 agtgagggaa aatccactca ctgaagggta aactccgtca agttatgtga ccagacggaa
    10621 ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc atagttctca aattgtttaa
    10681 aatgattgtc aagttaaaaa aatctgggat catataaaat agtatagttc tcaaatatga
    10741 ttagaaaccc tattaaagag caatattaag ccaacttact taaatcgttt aattaagaac
    10801 aataatctct tttagtcaga cttttagtct gacttttttg tatttccaaa atttattctt
    10861 tacaaaaacg aacatacgtt tgtataattg cttgtgaatg ggggaataaa aatgtttgat
    10921 tacagtagag aaccggtgaa tgactatttt ttaatcgaca tgaagtcttt ctatgctagc
    10981 gtggaatgta ttgaacgaaa tttagaccca atgactacac cgttaattgt catgagtaga
    11041 tcggacaaca ctggttcagg tctgactcta gcttcttcac ctactgcaaa gaaaaaatat
    11101 catattacta atgttagtag agctactgat ttaccttatc cattaccaaa agaacttcat
    11161 attgttccac cacgaatgaa cttatatata aaaaaaaata tggaagttaa tcagattttt
    11221 agaaagtatg tcgcagatga agatttattg atttattcga ttgatgaatc aattttaaaa
    11281 gtgacaaaat ccttaaattt atttactagt tcaggaacaa gaagcgagag aagaagagaa
    11341 ttagctaaaa taatcaaaga tgagattcag aaagaaacag gtctaatagc aacagtcgga
    11401 ataggagata acccattatt agctaaactt gcactggaca atgaagcgaa gagtagtaaa
    11461 gaatttatag ctgagtggac ttatgagact gtacctgaaa aaatatggaa aataaacgag
    11521 atgactgact tttggggcat tggatcacgc atgaaaaaaa gattgaatca tatgggaatt
    11581 atgagtatag aagatttagc aaattggaat ccctacacaa ttaaaagtca gttaggtgta
    11641 atcggactcc agttatattt ccataccaat ggaattgatc gtacggatat tgctattcca
    11701 ccagaaccaa ccaaagaaaa gtcttatggg aattcacaag ttctaccaaa agattatacg
    11761 aggagagaag aagtagaact agtagtcaag gagatggcag agcaagtagc cattcgaatt
    11821 agacaacaca attgcaaaac tggttgtgta cgcttagcaa ttggtacgtc aattttggaa
    11881 gaaaaatcag gtttctcgca ccagatgaaa ataccaatta cagataatac gaaagaatta
    11941 caaaattatt gtcttttttt attcaataag ttttatgagg ggcaggaagt acgacatata
    12001 ggagtaactt atagcagatt agtatacaca gattccctac aattagattt atttagtgat
    12061 ccaagaaaaa agattgctga agaaaatttg gataggatta ttgataagat tagacaaaaa
    12121 tatggattta cagctattat acatgcttca tctatgttag aaggtgcaag aagtattacg
    12181 agaagcactt taataggagg acacgcagga ggattaggag gtttgcataa tgattgaata
    12241 taacgataga ggacgtttaa aatggtcggg attttatttg tctgatcata ctgaaagaat
    12301 caatgcagac aatgtacaac gtgctaatca taatttggca aaagaacaaa tgacgactga
    12361 agagattgcg aaaatactga gaactgctgt cataaaaaat agacccgtct gcattcaaaa
    12421 agaggaacga aacgctgaag gttactatcc agatgatgta gtaggaaaaa ttattggata
    12481 tgacgagctt ggtgtatttg tggaagaaga aaaagttcac tatgatgaaa ttaggaatgt
    12541 ttctttctcg gaattattaa aatggagtca gatatgaaca ttattaaaaa atacgaattc
    12601 ggttatatta cgtatgaaca gctaatagag gaattatggg gatatggtca acaattaatt
    12661 aatgaagtgg ggattgattg cttttgtttc tatattgaat ccggagcagg ataccataag
    12721 aacagatact atatatttcc atatccacca aaatagtctc ttagatattt ttaaattttc
    12781 tccaaatact
//
LOCUS       DACCWV010000083        12234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCWV010000083 DACCWV010000000
VERSION     DACCWV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.083"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..1042)
                     /locus_tag="IVW84_001916"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IVW84_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IVW84_001917"
     CDS             complement(1437..2279)
                     /locus_tag="IVW84_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7918102.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IVW84_001918"
     CDS             2604..3020
                     /locus_tag="IVW84_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7918103.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IVW84_001919"
     CDS             3017..3487
                     /locus_tag="IVW84_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918104.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IVW84_001920"
     CDS             3501..4274
                     /locus_tag="IVW84_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918105.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IVW84_001921"
     CDS             4288..5100
                     /locus_tag="IVW84_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7918106.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IVW84_001922"
     CDS             5154..6119
                     /locus_tag="IVW84_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7918107.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IVW84_001923"
     CDS             6256..8979
                     /locus_tag="IVW84_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918108.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IVW84_001924"
     CDS             8992..9624
                     /locus_tag="IVW84_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7918109.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IVW84_001925"
     CDS             9829..10056
                     /locus_tag="IVW84_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP7918110.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IVW84_001926"
     CDS             10125..10391
                     /locus_tag="IVW84_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7918111.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IVW84_001927"
     CDS             10381..10650
                     /locus_tag="IVW84_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7918112.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="IVW84_001928"
     CDS             complement(10829..12130)
                     /locus_tag="IVW84_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7918113.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3990 a         1984 c         2400 g         3860 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
    12181 ctgtatgttt tgttttttca tgctcaaaag ggaccgatag aatgattttc tatc
//
LOCUS       DACCWV010000084        12200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCWV010000084 DACCWV010000000
VERSION     DACCWV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.084"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            621..1949
                     /locus_tag="IVW84_001929"
     CDS             621..1949
                     /locus_tag="IVW84_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7918114.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2151..2984
                     /locus_tag="IVW84_001930"
     CDS             2151..2984
                     /locus_tag="IVW84_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7918115.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3000..3737
                     /locus_tag="IVW84_001931"
     CDS             3000..3737
                     /locus_tag="IVW84_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7918116.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3892..4860)
                     /locus_tag="IVW84_001932"
     CDS             complement(3892..4860)
                     /locus_tag="IVW84_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7918117.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5092..5925
                     /locus_tag="IVW84_001933"
     CDS             5092..5925
                     /locus_tag="IVW84_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7918118.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5959..6798
                     /locus_tag="IVW84_001934"
     CDS             5959..6798
                     /locus_tag="IVW84_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7918119.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6824..7384
                     /locus_tag="IVW84_001935"
     CDS             6824..7384
                     /locus_tag="IVW84_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7918120.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7583..9304
                     /locus_tag="IVW84_001936"
     CDS             7583..9304
                     /locus_tag="IVW84_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7918121.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9469..10611
                     /locus_tag="IVW84_001937"
     CDS             9469..10611
                     /locus_tag="IVW84_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7918122.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10627..11838
                     /gene="thiI"
                     /locus_tag="IVW84_001938"
     CDS             10627..11838
                     /gene="thiI"
                     /locus_tag="IVW84_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7918123.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2048 c         2535 g         3462 t
ORIGIN      
        1 taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc
       61 acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta
      121 tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca
      181 tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga
      241 aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa
      301 aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc
      361 cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc
      421 tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat
      481 tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa
      541 tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt
      601 ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca
      661 aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa
      721 ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag
      781 atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg
      841 cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat
      901 cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca
      961 tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc
     1021 ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg
     1081 tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact
     1141 acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag
     1201 aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct
     1261 tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa
     1321 agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg
     1381 taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc
     1441 gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga
     1501 atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg
     1561 tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag
     1621 atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt
     1681 tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag
     1741 acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg
     1801 gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa
     1861 aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc
     1921 cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa
     1981 gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt
     2041 atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat
     2101 taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc
     2161 aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg
     2221 ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga
     2281 atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg
     2341 tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg
     2401 aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc
     2461 atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc
     2521 agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc
     2581 agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc
     2641 gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc
     2701 acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg
     2761 acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg
     2821 gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga
     2881 aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag
     2941 aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt
     3001 tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa
     3061 cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc
     3121 agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt
     3181 attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag
     3241 ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga
     3301 aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga
     3361 gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc
     3421 gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt
     3481 atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg
     3541 tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata
     3601 aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg
     3661 gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta
     3721 tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa
     3781 gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag
     3841 ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg
     3901 gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa
     3961 acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt
     4021 tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag
     4081 tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag
     4141 attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc
     4201 caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc
     4261 ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt
     4321 tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt
     4381 ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc
     4441 tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact
     4501 tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat
     4561 cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac
     4621 tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga
     4681 aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt
     4741 ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt
     4801 gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat
     4861 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     4921 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     4981 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     5041 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     5101 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     5161 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     5221 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc
     5281 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     5341 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     5401 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     5461 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     5521 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     5581 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     5641 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     5701 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     5761 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     5821 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     5881 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     5941 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     6001 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     6061 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     6121 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     6181 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     6241 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     6301 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     6361 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     6421 gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca
     6481 gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat
     6541 actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat
     6601 gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag
     6661 atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat
     6721 gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac
     6781 ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc
     6841 ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac
     6901 ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga
     6961 atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca
     7021 cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc
     7081 gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc
     7141 attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa
     7201 attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga
     7261 aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat
     7321 tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga
     7381 gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt
     7441 ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg
     7501 agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat
     7561 actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat
     7621 cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga
     7681 tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga
     7741 agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg
     7801 gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt
     7861 cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga
     7921 agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa
     7981 ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta
     8041 tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga
     8101 attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac
     8161 ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga
     8221 agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc
     8281 tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt
     8341 cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt
     8401 agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga
     8461 tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa
     8521 aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt
     8581 attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg
     8641 tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc
     8701 acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt
     8761 caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt
     8821 gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag
     8881 tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt
     8941 cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt
     9001 aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga
     9061 taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc
     9121 aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt
     9181 attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg
     9241 agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt
     9301 ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc
     9361 tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct
     9421 tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt
     9481 gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt
     9541 cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg
     9601 ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat
     9661 tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa
     9721 agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca
     9781 gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga
     9841 tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt
     9901 atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg
     9961 gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca
    10021 ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat
    10081 ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg
    10141 acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg
    10201 gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca
    10261 caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt
    10321 tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc
    10381 gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc
    10441 tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg
    10501 gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa
    10561 tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt
    10621 gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag
    10681 aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt
    10741 ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac
    10801 gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt
    10861 attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa
    10921 atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa
    10981 ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat
    11041 atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc
    11101 gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga
    11161 aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg
    11221 aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa
    11281 gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat
    11341 tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga
    11401 tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt
    11461 cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg
    11521 caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca
    11581 atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc
    11641 cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa
    11701 ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca
    11761 ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa
    11821 ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac
    11881 aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg
    11941 attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa
    12001 taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc
    12061 atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga
    12121 aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga
    12181 acaccgtgta tcagaggttg
//
LOCUS       DACCWV010000085        12144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCWV010000085 DACCWV010000000
VERSION     DACCWV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.085"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..566
                     /locus_tag="IVW84_001939"
     CDS             <1..566
                     /locus_tag="IVW84_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7918124.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="IVW84_001940"
     CDS             967..1572
                     /locus_tag="IVW84_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7918125.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="IVW84_001941"
     CDS             1588..2160
                     /locus_tag="IVW84_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7918126.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="IVW84_001942"
     CDS             complement(2412..2669)
                     /locus_tag="IVW84_001942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918127.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="IVW84_001943"
     CDS             complement(2795..3610)
                     /locus_tag="IVW84_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918128.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="IVW84_001944"
     CDS             4127..5452
                     /locus_tag="IVW84_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7918129.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="IVW84_001945"
     CDS             5445..5795
                     /locus_tag="IVW84_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918130.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(6866..7141)
                     /locus_tag="IVW84_001946"
     CDS             complement(6866..7141)
                     /locus_tag="IVW84_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7918131.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="IVW84_001947"
     CDS             complement(7113..8066)
                     /locus_tag="IVW84_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7918132.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="IVW84_001948"
     CDS             8678..10171
                     /locus_tag="IVW84_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7918133.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="IVW84_001949"
     CDS             10306..10602
                     /locus_tag="IVW84_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7918134.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="IVW84_001950"
     CDS             10703..11554
                     /locus_tag="IVW84_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918135.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="IVW84_001951"
     CDS             11575..11787
                     /locus_tag="IVW84_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918136.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DACCWV010000086        12142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCWV010000086 DACCWV010000000
VERSION     DACCWV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.086"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            304..534
                     /locus_tag="IVW84_001952"
     CDS             304..534
                     /locus_tag="IVW84_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918137.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            693..1526
                     /locus_tag="IVW84_001953"
     CDS             693..1526
                     /locus_tag="IVW84_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP7918138.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1519..2934
                     /locus_tag="IVW84_001954"
     CDS             1519..2934
                     /locus_tag="IVW84_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7918139.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            2946..4475
                     /locus_tag="IVW84_001955"
     CDS             2946..4475
                     /locus_tag="IVW84_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7918140.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4567..5445
                     /locus_tag="IVW84_001956"
     CDS             4567..5445
                     /locus_tag="IVW84_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP7918141.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5447..5764
                     /locus_tag="IVW84_001957"
     CDS             5447..5764
                     /locus_tag="IVW84_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918142.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            5813..6514
                     /locus_tag="IVW84_001958"
     CDS             5813..6514
                     /locus_tag="IVW84_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP7918143.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6529..7419
                     /locus_tag="IVW84_001959"
     CDS             6529..7419
                     /locus_tag="IVW84_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP7918144.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7442..7657
                     /locus_tag="IVW84_001960"
     CDS             7442..7657
                     /locus_tag="IVW84_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918145.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7669..8010
                     /locus_tag="IVW84_001961"
     CDS             7669..8010
                     /locus_tag="IVW84_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918146.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8010..8381
                     /locus_tag="IVW84_001962"
     CDS             8010..8381
                     /locus_tag="IVW84_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918147.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8374..8769
                     /locus_tag="IVW84_001963"
     CDS             8374..8769
                     /locus_tag="IVW84_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7918148.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8771..9148
                     /locus_tag="IVW84_001964"
     CDS             8771..9148
                     /locus_tag="IVW84_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918149.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9149..9757
                     /locus_tag="IVW84_001965"
     CDS             9149..9757
                     /locus_tag="IVW84_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7918150.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9757..10098
                     /locus_tag="IVW84_001966"
     CDS             9757..10098
                     /locus_tag="IVW84_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918151.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10340..>12142
                     /locus_tag="IVW84_001967"
     CDS             10340..>12142
                     /locus_tag="IVW84_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP7918152.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAIN"
BASE COUNT         4405 a         1687 c         2499 g         3551 t
ORIGIN      
        1 agacgctaag catgtgtgct aggtcaatgc ttcggcaacc atgcaaggtt cgattccttg
       61 ccagtgactt tggcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt
      121 acgcgttttg tgcatcgtgt aggttgctat tacataactg gttaggttag attgagattt
      181 gggattcggt acaaatgaat cgtcaaatgt ctcaagcaca ggatcggaaa cgtccctgcc
      241 tgtgcattac atattagatc actctttgag tggtcttttt ctttacataa aggaggctac
      301 ataatgagaa actactggta tgtatcgcta actaatgaat atcctcgaac cattgatgat
      361 tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga agtactctat tgtcgaaatg
      421 gggagagaag cagaaccatg tgagattgat gcgtgcaagc tcgtttattg cggtcatggt
      481 ttctatgatg aaccaaacat tcaaaataat attaacaaga atttgaggga ttagaatgca
      541 agaaatggct taaaaatctc caatcaggac aaatcaagtt agacaaagtt tccgatttaa
      601 agatattgat tgaagctgat ttaatgctaa aagatattga aaattagaaa acaaaactca
      661 acctaaacag aatgtgaggt ggtgtgtatt gaatggcaag acaacgtgat ccaagacgtg
      721 atgaagccaa aagaatttgg ttagaatcca acggagaaaa gcagttaaag gaaattgcat
      781 ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa atcgcaagac aaatggagcg
      841 ctgaattgat tagtaacgtt accaatggca aaagtaacgt tactaatcaa ggtggcgctc
      901 ctattggtaa tcaaaatgct aaaggtaaca aaggaaatag ccgagcttct ccgccagtgg
      961 gtaataaaaa cgctttgaaa acaggtgagt atgaaacaat attttttgac acgttaaccg
     1021 ataatgagaa ggacatctat tctagtttga atgatgatcc ttcttttgct ttgtccgaag
     1081 aaatacgact tcttaaaata agacaacttc gaatgatgaa aagaatccaa caagctgaag
     1141 ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct aagaaagatt aaaactccga
     1201 ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt tatgcaagat gtgcagatta
     1261 gcagaaaaaa acatcgcaaa attgatgata ttctttcaat tgaagattca ttgactcgga
     1321 ttagcaacca gttagctaaa gccatcaagc aaatgaatga actttatatg aatgaataca
     1381 gaactgattt aattaaagct cagactgata agatccaagc tgagacaaat gaaattggcg
     1441 gaaataattc aggtgaagaa atagaagaat ggaaacaggc agttttaaat gccgcaaaca
     1501 aacgggcggt gaaagaaaat gaataatgaa tttattcctt ttgctgatat tggttctgcc
     1561 attgattatt actatgataa accagtagct ttttgccaag atattttgca tttgaatcct
     1621 gatgaatggc aagaaaatgt tttaaatgat ttagctgaat tttcaaaggt ttctgttcgt
     1681 tctggtcaag gagttggaaa aacagcatta gaagcaggag caatactttg gttcttaacg
     1741 tgtcgaccct acgctaaagt aatagcaaca gctccgacaa tgaagcaact ttacgatgta
     1801 ctttgggcag aggtagctaa atggttaaat gatagcttga tcaaaaactt actgaagtgg
     1861 acaaagacca aaatttatat ggttggtgat tcagagcgtt ggtttgctac ggctagaaca
     1921 gcgactaaac cagaaagtat gcaaggtttt cacgaggacc atatgttgat tgtggtagat
     1981 gaagcttctg gtgtgtctga tccaattatg gaagctattc ttggtacgct atcaggtttt
     2041 gataataagc tgttgatgtg tggaaacccc aataatattg aaggtgtctt ttatgattcc
     2101 cataattcag accgtgataa atacagagtt cataaagtat caagctatga tagtaaacgt
     2161 acaaacaaag acaatataga aatgattctt aaaaaatatg gaaaagaaag tgatgttgct
     2221 cgtgtccgta tttttggaga atttccgaaa ggtgcgttgg attcatttat cagtcttgaa
     2281 acagttgaat tggctacaga aaaacaaatt agtgattctt tagtcaataa aacaaaggtt
     2341 gctcatattg gtgtcgacgt agctcgatat ggtgatgatt ctacgattct ctttcctaga
     2401 attgctacca gggcattgga gtatgagaag tattcaaaac gtagcaccat ggaaacaaca
     2461 ggatatgtca tcaacatggc caagaatcta atgagtcaat atccgagtat tgataaagtg
     2521 atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa ccgaccgcct agaagaactt
     2581 atagaagaca aacattatcc ttttgaggtg tttggagtga ataacggttc aacatcagaa
     2641 gacgattttt acgataattt aggtactcaa ctatggggaa acatcaagga aatgttagaa
     2701 gaaaatatga cagcaaatct taacggagaa cagcctgtta ttgaattgcc ttctgatagt
     2761 tcgttaatca aagaattaag tactcgcaaa ttcaaaatga caagtagaag tcgtatacgt
     2821 ttagaaagta aagatgatat gaaaaagcga aatattggta gtcccgatat tgctgacgca
     2881 ctggctttag cgttttatga gccaccaagt cactatcaat ttattcaatt ttaggaggtg
     2941 agcttttgat tagtacagta ttatctttag agagatataa gaagctacgt gttaaatatg
     3001 caacacaaat tgaagatgga atgtttgatc cgaatggttt tatagaagat atgaaaccat
     3061 tttttgctga tcgcgaaaga aaatatctag cttatactag cgaaaagaat gaaatagata
     3121 atagaccaaa gcctaacact gatattgtaa aagttaataa taaacttcat gctggtatgt
     3181 attcaattgt cgtagaccaa gcagtcaatc attttactgg tatacctatc aaatgggatt
     3241 acgatgtatc ggaacaaaaa agaacactca ttcaaagatt aaaagataaa ttcttaaaaa
     3301 atgatataga acttccgaca gttccagaag cttttaacaa attaacaagc aatcttgatt
     3361 ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt tcaaggagct tgtggagtag
     3421 ctttccgatt gctagagcct gtggaagaag atgatggttg gaagttaaga gcaagtaata
     3481 ttgaaccttg gagagcagaa agatatggaa atgccggaat ctatatcaaa gaaaagtatg
     3541 actcttacca gaaaaaattt tttcaagaaa tgaaagttat gacaagaaat aaaattctga
     3601 catatgcttg ctatggtgat ttgaattttg ttacaagcgg aacgttcaaa aaaattgatg
     3661 agaccgataa ccctttgggg acgattacct tggcagaatt taaaaataat acgaatcgtt
     3721 attgtgattt tgaagtagct gaggaaattg gagatgcaat tgatcgtgct ttgtctgatc
     3781 aacaaaacga aatcgaacaa tttaagcttg cgtacatgct tgttactggc acaacaatga
     3841 gcaaaggcac tgctaaagaa atgatgaatc agctagggat tatcaactta aaagccccta
     3901 ctgcaaaagc tgaatatgtc acaaaaaatt tagcaaaaga tttcaatgaa tatcatatgg
     3961 atctattgaa aaaacagttt tacaccattt gtaaagcaat cgattttaac gatgaggtgt
     4021 ttaaatcaaa cagttctgga gaagctcgca agtggcaaat cattagccta gaagcgaaaa
     4081 caaatactaa agagcaatat tttagagaag gattgaaaga atgcgcagaa acaattgctg
     4141 cttttcttaa gtttcatgac aaagtagaaa ttgagcctga aaaaattatt tttactttct
     4201 ctagatcttt gccaactgat ttaagttatt tagctgaagc tttacctaaa cttgcaccat
     4261 acgtatccaa acgaactatt cagagccaaa ttccatttgt aacagatgtt gattatgaaa
     4321 atgaaatgat ggaattggaa agtggaagtg cttatccaga tagtgaatac aattttggcg
     4381 gaggtggcaa tagtgacgat agaaacgaag tattggacca aacgtcgaga actggaggat
     4441 caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat taactagtgt atttcctgaa
     4501 gcactgaaag agatacaagc aaaactatta tcacaagctg acttacacaa tatcacttat
     4561 ccagaaatga tggagtttta cagtacaagt aatcagaaaa aatatcgtga atatgtggaa
     4621 aaaaattata agtcattaaa aatgtatgat gcaaaataca aagagtttat cgatgaattt
     4681 tttccaccat ttgactatgc aaaagtcaat cgcttattac aaatacgatc agatgtattt
     4741 aaaattcttg cagaatatgc gatggatgca gatgtgaatc aatatttttc tgatcgctta
     4801 gaggaaattc ttcaaagaac atattcttct aatgctaatg tttttgttca acttttaaac
     4861 gttgatatac caaattattt accagaaaat gaaattaaac attatttgaa ctatccatgg
     4921 tcaggaaaga cattttcaag aagactttgg ggaaatattt catcgcttga acagaaactc
     4981 tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg ttatacacgc attaaacacc
     5041 atgagattag actcagaaat ttgtgacatg tttaagttag aagaatcaaa gtataacaaa
     5101 gcgatagaaa atctcgttcg aacggagtat gcaaaatttg cacaagatgg tattgaaaaa
     5161 tcatatttag aaacaggtat tgaggaatac aacgtattga ctgcaaaaga tgagagagtt
     5221 tgccggattt gtggaggaaa ggcaagtaag aatccctata aactgaaaga tgctgtcata
     5281 ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta cggatgttcc taatttacca
     5341 aaattaggaa aggatattga tgaagaatat gaccgtttat ttggcgattt attagatgag
     5401 tttgcacatg attcttttgg aattaatttg aaacggagga agtagaatga aagatttttt
     5461 tgaagcagta ctaacaatta atgtaaatgc tgatattgca gaagcctaca aaacagctat
     5521 tgagtctgag aaccatccta atggcttgag agaccattgg aatggcaatt atgcctacgt
     5581 ggttattggc gatcaaactg ttaattatca agataatact ccagttgata agaataccgt
     5641 taatttaacg attcaattat tatctcattc attaccaaat ttaaaagaaa cagttgattg
     5701 gtatgaaaag atgggatgta ttgttgttag aactgactac aaagaaggaa agtctagtaa
     5761 ttaggctttt ttattttgtc cgaaatgacg ttaaactagc gcaatactgg gcttggttga
     5821 atggtggggc gcaactatta aaactcaaag caatgcgggg cgtgaaaacg aatcgtgggg
     5881 cgaaaggaga atgagcatga aacacaaatc attaatgcca atgaatttgc aatactttgc
     5941 cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa tcttttgtgg aatccaatga
     6001 agaagctcaa aaatttattc agtcacaatc tcaaagtgtt gccgacaaac aattggaagc
     6061 ttggaaacaa aataacttag ataaaattaa acaggatacc atcaaggaat atgaggaatc
     6121 taagaaaaac aagtcacctg agcaaattca actggaaaaa ctacaagctg aatttgaagc
     6181 agaaaaagcg ttgcgtgtga caagtgataa taaagcattt gttgcagaac aaattgctgg
     6241 attagaacta gatggagagt taaaagagtc tatttctcaa tttatgctaa ataatcttgt
     6301 tagttcggat acagatttca ctaagaatgc tgttgaaggt tttacaagtg ttttgaatgc
     6361 aattaatgag aaacatgcag atgcattaaa agaactacaa atgaagtctg catttggtgg
     6421 aactcaacaa tcgaataacc aggttcagca gaacaatgaa acattaacaa atccagaaga
     6481 acaattagga caaattcttc aacaatttaa ctaggagagt gaaaaattat gaaaaaaaca
     6541 tctttaaata atttagagta tttggatatt tcaccagcga ttaatgctat gcaagtacca
     6601 aatacacctt ttttaagcta tttacttggt gctggaaaaa cagagccagc aaactcgaca
     6661 gaaattaaat ggcgtgaata tgatatcaac aacgatgatt cttctgaaaa acttgagggc
     6721 ggagaatatc cagatgctga atcaggtcga acttggttta acaactatac tgaaattttt
     6781 agaaaatcaa cctctgtatc tggtacatta gatgctatta atgtgaatgg agtcggaaat
     6841 gaattaacta atcaagtagc actacgtggt atggaaatga aaattgattt gaaccgaaaa
     6901 ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag gtcgtcgaat gaatgggatt
     6961 ttgaacttga tcaattcagc aaataaggca gaaacagcca ctgcgggtgc agtaacaaga
     7021 aaagatatcg atactttatt taaattgatg tatgaaaaag gttatatggg agaaaaacta
     7081 tgcttgattt ctccagatat gcaggagtta atgactgatg agttagatgg aaaatcaaca
     7141 aaaattgttc agttcgggga aagagtaact tttggattgc aaattggaaa tatcgtgtct
     7201 aattacggta caggtattgc tctactagaa ccatcattgc caaaaggaac aattgccgca
     7261 atcgatacta attatgtgaa actacgtcca ttacgtgaat ggagagcaga agaacttgca
     7321 aaaacaacgg attctagacg tatcggtctt gtaggtgaat actctcttga atacaatgct
     7381 tcaaactctg gggcaatttt aaatttaaag tctgaataaa agggagttag tactcccttt
     7441 tttgatagga ggaattacag tggtaaaaaa agatgaaact aaaaaagatg aagtcgtgaa
     7501 atatagagta ggtaaaacta aaaattttgt tgggtttgtt catcctaaaa ctcgtagatt
     7561 tatcacagca gattcaaata acgaatttat catttctata gatgataaag aagcaattgc
     7621 aattttggaa gatgcaattg atgttaatga aatttaggaa gtgatctgat ggatgaatcg
     7681 ctaaaaacgg aaatcattga gtctacaaaa gaagattttc cagatttgag tgaagaacgc
     7741 ataactaatt tattagaaat aattttgcta gaaattgaat catacaacac ttgtaaaaat
     7801 gatatttcat gggaaaagtt aaaaagcgtg attagtgaag tgttgtataa aataataaaa
     7861 aatgaatcag aaaaaacagt atcttcagtt agacgtggcg atacaacgat tagttatgct
     7921 tcaacaacaa atgatgttag tgagttactt ctaggctatg gtgatttgat acgaagagtt
     7981 attggttgtg gaggattgga gtttttttaa tgaatgaagc agatatttta gaaactactt
     8041 atgaagatag ttgtattatt gaaagactga cggacattga agattctaat acaaatatta
     8101 ctattcaaga ttacaaaaaa gtatatgata atcctatttc ttgcgctctt tcacaaggtc
     8161 aaatcgatgg actagcagtc atagaagatg gagagatggt aaatgtttca actgacacat
     8221 ataaattatt tgttcatcct aagattaaac taaaaaaagg agatcgaata acaataactc
     8281 aaaaggccag tggcttaatt ttttctctat ttgctactaa gcctttttac tatcctagtc
     8341 attgtgaagt gaatttgata ggaagtgaga aaaatgggta atctcaaata tgaatcaaat
     8401 gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa ttgctcaaaa agaaggaata
     8461 tccttcgtta acgattcaat gaataaagtc gttagtttag ctaaaccatt aactcctgta
     8521 aaatcaggta atttaaggcg aggatatcgt gtagtaaaag ctagaaaact atcaagcggt
     8581 cgaattgttg gagaagttat aaacaatgaa cattatttta aatatgtaga agaaggtcat
     8641 aggactaaaa atggtggctt tgtcaaaggg aaattcatgt taactcgtgc aacgaatctt
     8701 gcaaatatgt cttatattcc tcgaagattt aaacaaatgg caataaaaat cgttaagaaa
     8761 ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt agcaaattaa aagaaatcta
     8821 tcctgatggg acaatatatc ttgattcagt tatgcagtca actaaagatt tttatttcgt
     8881 tttatcagta atggaatctg gaactgaaaa tgtaggaatt gatattcaaa atgtttcttt
     8941 cttaattgat attgcattga ttgataataa ccctaataga aatttaatta atgaattagt
     9001 ctcacgttgt gggacttttt ttaatacgat tacaatcgat gagcaaatat tatttccaaa
     9061 agcctattta cctgatgaag cagatggtgt tcaacatatt cgttttacat tagaatttcc
     9121 acaatatatt gaatggagtg aaagataaat gggagaaaaa agaagtaaaa ccggaattat
     9181 ttctgtcgaa aaacctactt ggtttccttt ggaagatgag acagggaatt ttccggtata
     9241 tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa ccaacagcta attatgaaac
     9301 aacacaagat tatggggatt cagtggttca agatcaattt acagcttttg gtggtgcaga
     9361 agttgaactg gaagcaaatg gatatactca taaagtttta tctacaatta ctggagggaa
     9421 aattgttaaa ggtggtgcct tgcgttctgg agaagatatt gcgcaagatg gtgcgtttgc
     9481 atatagacgt aaaaaatcga atgggaaata ccgttatacg gtcttttata aaggacagtt
     9541 tgctctagat tcagatgaaa catcaacgat tgaaggaagt aaagtaagtt ttactcatcc
     9601 aacttggaag ggttcatttg tagatgtacc aggacttgga tatatgtatt cagttgatga
     9661 agatgatgaa ggcgtggata aagcaatgat cggaaattgg tttacaaaag tagcaattcc
     9721 aattgaagaa gcagaacttt caggaggtac agaataatgt caaaatatca aacaacgatt
     9781 aaattaacga aaaaagacga agaaggtaaa tatgagcaag tacaatttaa atctgctgaa
     9841 tttttaccag gaacagttgt agaagatgct gcaggagtta tggaagaaat gcaaacagcg
     9901 actgataagc aatcagttaa aaaagcttta agtcgtgctt attcatttat tgcagatact
     9961 ttatttgagg gacaatttac tggtgaagat tattgcaaag gaattgatgc tcgtgagatt
    10021 gcctcattga caggaaaatt attgaagtct gttactgcag gttttgatga aacttataca
    10081 gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc atttaagtat tcgattactt
    10141 accgagaatt agatataaag acgcaattac tcgaagcagg ttggacgtta ccagaaattg
    10201 aacgtactga cttggatgag ttaatgcgtc tttttgcttt tagagatgcg gtaaaagagc
    10261 atgaagatgt tgagtactat gataatttca ctcaatttta ggaggtgata ctttgaataa
    10321 cgatgaccta attctgaaga tgatactaga tgaatctggt tttactgccg gtatgaataa
    10381 tgctgtcaaa aagttgaact cttttgatga aacaatcgaa agaaaaagta gaaatagtgg
    10441 cagttcatta ggtagtattt ggaaaatatt tgctggaagt tttctagcta gcggagtaac
    10501 tagaatcgta ggagctggct ttgatctaat caagggttcc ataagtggag cggttgatcg
    10561 agtagatacg atgaataacg ctctacgaaa tttccaaaat atgggattta gtaattcaga
    10621 aattatgaag aatatcggaa agaatgggct tttatctcaa ggtattcaag gacttcctac
    10681 tgctttgaat gatgcgataa gtcatgttca acttcttgct tcttctacag gtgatttaac
    10741 tcgttcaact caaatattta aagctttaaa tgatggaatt cttggtttcg gtggttcaac
    10801 tgatcaagtt aatgaagctg ttattcaatt atctcaaagt ttctcaaacg gaaaagtaga
    10861 cgcacaaact tggaactcaa tgatcaacgc tcaacttgga cctactcttt ctgctattgc
    10921 taaaaagatg ggaattacaa tgggagatct gaaagaaggt ttatctcaag gtaagatttc
    10981 tgttgaagaa ttccaaaatc aattaataga aatggatacc aaaggtggcg gaggacttaa
    11041 atcattaagt caaatcgcta aagattctac taagggaatt aaaacctcta tacaaaatgc
    11101 aaaaacagct gttacacgtg gtgttggtga ggtaatagaa ggattaaata aagcattagt
    11161 agattcagat ttaggtggat ttaagggaat tattgataaa gtgggcagtt caatggaatc
    11221 gttcttaaaa gtaattgctg caaatattcc tacagcagta tcatttttaa gtaatttatt
    11281 taacgaaatt caaaaatttg gttctgcttt gaaattcatg atgccctttc ttgttcctgc
    11341 agctaccgct tttggagcgt tcatgtttca acttaaaggt ataccagcaa taataaaaag
    11401 cttcaataat tttaagaatg ccataattgg cgttggaaat tcattaaaga ttatgggggc
    11461 aatagctgct gctaatccat ttgttttaat tattggtgca gtcgttggtg caatagctgt
    11521 tttcggttac tttatggcaa ccaatgaaga gcttagaaac aaggttatat ctatttggaa
    11581 tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata aaggactggg gaattgatac
    11641 ttggaattct gctaaagaaa tggcatcaaa tgcagttgag ggtgtcaaag atgcttggtc
    11701 aggaacaaag gaatggtttt ctaacacctg gaaaggtatt aaggatgggg ccacaggttt
    11761 atttgataaa acagtagaga cttcaagaaa tgcagtcgat agtgtaaaaa atgcatggtc
    11821 taatatgaag aaatggtttt ctgatacttg gcaaagtata aaagattcag ccagagaaaa
    11881 atggaatgga atcaaaggtt ctattatgtc agttgctggt ccattgatta caggtataaa
    11941 gaatgcattt ttacatgtta ctttttatct agagacctta tggaataatt tagttgagat
    12001 tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt gcgccggttt tgtttattac
    12061 ctccatgatt tctggtggat gggaagaaac aaaaaataat atgattgctg tttggaataa
    12121 tattaaagaa agtgccataa at
//
LOCUS       DACCWV010000087        12084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCWV010000087 DACCWV010000000
VERSION     DACCWV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.088"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(32..589)
                     /gene="frr"
                     /locus_tag="IVW84_001968"
     CDS             complement(32..589)
                     /gene="frr"
                     /locus_tag="IVW84_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7918153.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(592..1314)
                     /locus_tag="IVW84_001969"
     CDS             complement(592..1314)
                     /locus_tag="IVW84_001969"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7918154.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1450..2331)
                     /locus_tag="IVW84_001970"
     CDS             complement(1450..2331)
                     /locus_tag="IVW84_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7918155.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2430..3212)
                     /gene="rpsB"
                     /locus_tag="IVW84_001971"
     CDS             complement(2430..3212)
                     /gene="rpsB"
                     /locus_tag="IVW84_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7918156.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3571..4050
                     /locus_tag="IVW84_001972"
     CDS             3571..4050
                     /locus_tag="IVW84_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7918157.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4267..5358
                     /gene="phnW"
                     /locus_tag="IVW84_001973"
     CDS             4267..5358
                     /gene="phnW"
                     /locus_tag="IVW84_001973"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7918158.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5351..5479
                     /locus_tag="IVW84_001974"
     CDS             5351..5479
                     /locus_tag="IVW84_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7918159.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5483..6028
                     /locus_tag="IVW84_001975"
     CDS             5483..6028
                     /locus_tag="IVW84_001975"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7918160.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6482..8173)
                     /locus_tag="IVW84_001976"
     CDS             complement(6482..8173)
                     /locus_tag="IVW84_001976"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7918161.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8593..9540)
                     /gene="arcC"
                     /locus_tag="IVW84_001977"
     CDS             complement(8593..9540)
                     /gene="arcC"
                     /locus_tag="IVW84_001977"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7918162.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9655..10674)
                     /gene="argF"
                     /locus_tag="IVW84_001978"
     CDS             complement(9655..10674)
                     /gene="argF"
                     /locus_tag="IVW84_001978"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7918163.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10765..11994)
                     /gene="arcA"
                     /locus_tag="IVW84_001979"
     CDS             complement(10765..11994)
                     /gene="arcA"
                     /locus_tag="IVW84_001979"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7918164.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3294 a         2517 c         2192 g         4081 t
ORIGIN      
        1 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
       61 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      121 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      181 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa
      241 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      301 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      361 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      421 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      481 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      541 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      601 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      661 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      721 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      781 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      841 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      901 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
      961 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1021 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1081 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1141 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1201 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1261 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1321 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1381 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1441 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1501 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1561 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1621 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1681 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1741 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1801 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1861 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1921 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     1981 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2041 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2101 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2161 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2221 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2281 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2341 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2401 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2461 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2521 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2581 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2641 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2701 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2761 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2821 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2881 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     2941 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3001 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3061 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3121 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3181 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3241 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3301 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3361 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3421 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3481 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3541 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3601 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3661 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3721 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3781 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3841 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3901 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     3961 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4021 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4081 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4141 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4201 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4261 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4321 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4381 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4441 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4501 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4561 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4621 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4681 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4741 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4801 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4861 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4921 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     4981 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5041 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5101 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5161 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5221 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5281 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5341 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5401 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac
     5461 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5521 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5581 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5641 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5701 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5761 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5821 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5881 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     5941 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6001 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6061 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6121 atgaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6181 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6241 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6301 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6361 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6421 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6481 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6541 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6601 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6661 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6721 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6781 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6841 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6901 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     6961 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7021 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7081 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7141 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7201 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7261 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7321 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7381 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7441 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7501 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7561 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7621 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7681 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7741 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7801 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7861 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7921 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     7981 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8041 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8101 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8161 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8221 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8281 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8341 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8401 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8461 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca
     8521 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8581 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8641 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8701 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8761 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8821 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8881 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     8941 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9001 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9061 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9121 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9181 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9241 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9301 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9361 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9421 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9481 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9541 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9601 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9661 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9721 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9781 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9841 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9901 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
     9961 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10021 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10081 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10141 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10201 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10261 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10321 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10381 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10441 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10501 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10561 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10621 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10681 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10741 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10801 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10861 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10921 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    10981 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11041 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11101 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11161 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11221 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11281 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11341 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11401 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11461 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11521 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11581 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11641 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11701 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11761 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11821 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11881 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    11941 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12001 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12061 ttttgtctca ttcaaaatga gaca
//
LOCUS       DACCWV010000088        11769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCWV010000088 DACCWV010000000
VERSION     DACCWV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.089"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(158..535)
                     /locus_tag="IVW84_001980"
     CDS             complement(158..535)
                     /locus_tag="IVW84_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918165.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(835..1716)
                     /gene="galU"
                     /locus_tag="IVW84_001981"
     CDS             complement(835..1716)
                     /gene="galU"
                     /locus_tag="IVW84_001981"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7918166.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1759..2778)
                     /locus_tag="IVW84_001982"
     CDS             complement(1759..2778)
                     /locus_tag="IVW84_001982"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7918167.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3411..4415)
                     /locus_tag="IVW84_001983"
     CDS             complement(3411..4415)
                     /locus_tag="IVW84_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7918168.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4428..5477)
                     /locus_tag="IVW84_001984"
     CDS             complement(4428..5477)
                     /locus_tag="IVW84_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7918169.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5494..6315)
                     /locus_tag="IVW84_001985"
     CDS             complement(5494..6315)
                     /locus_tag="IVW84_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7918170.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6302..7081)
                     /locus_tag="IVW84_001986"
     CDS             complement(6302..7081)
                     /locus_tag="IVW84_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918171.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7098..7568)
                     /locus_tag="IVW84_001987"
     CDS             complement(7098..7568)
                     /locus_tag="IVW84_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918172.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7580..7999)
                     /locus_tag="IVW84_001988"
     CDS             complement(7580..7999)
                     /locus_tag="IVW84_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7918173.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8080..10848)
                     /locus_tag="IVW84_001989"
     CDS             complement(8080..10848)
                     /locus_tag="IVW84_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918174.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11048..11272
                     /locus_tag="IVW84_001990"
     CDS             11048..11272
                     /locus_tag="IVW84_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918175.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3413 a         2479 c         1908 g         3969 t
ORIGIN      
        1 ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta
       61 tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc
      121 cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc
      181 tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta
      241 tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata
      301 aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc
      361 caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa
      421 acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca
      481 tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga
      541 acacacacct ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg
      601 cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa
      661 ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc
      721 acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag
      781 ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact
      841 ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc
      901 cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc
      961 gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag
     1021 tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa
     1081 tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat
     1141 ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg
     1201 atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga
     1261 agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat
     1321 caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag
     1381 cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt
     1441 cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa
     1501 ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa
     1561 gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc
     1621 cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag
     1681 cccagcggca ggaatgactg cttttttgac cttcacttgc tgttcctcct aaacgacgat
     1741 taactattga tcatctattt aacgaagaga aaattcattc tccatttttc cgtcacgtaa
     1801 catgatttct tttgctgctt ttttgatatc ttctccatta tacaaaacat tatagattgt
     1861 ttgagtaatt ggcatctcaa cacctagctg ttgagctagc tctactgctg cttttgtcgt
     1921 tgccacaccc tcaacgacca ttcccatgtt ttctagaact tcttccagct tatgtccctg
     1981 tcctaacaag ttaccggcac gccaattgcg agaatgaaca cttgtacaag taacgatcaa
     2041 gtcccccaca ccactcaaac cgataaatgt cagaggattg gcacccatcg cgacaccaag
     2101 acggctgatt tctgccaatc ctcgagtcat gattgctgct tttgcatcat ctccgaatcc
     2161 taatccatgg atagcgccgg caccaattgc aatgatattt ttcagtgctg caccagtttc
     2221 tacgccaatc acatcattat ttgtataaat cctgaaataa tcattcatga acagtttttg
     2281 tacataggct gctgcttttt gatcaatact tgcagctgta attgttgtga tatcatgtac
     2341 tgccacttcc tcagcatggc taggaccaga taatacaaca attgcctgtc ttttttcttc
     2401 tggaatttct tcagccaaaa cttctgagat tcgtttatgc gtatcttgtt ctagtccttt
     2461 acttgcatgt ataatgattg gcttagtatt cattttacca ataagttcct gtgcgactga
     2521 acgaatcgct tttgttggaa caacaaataa aatagcatct gcgttatcca ctgcttctgc
     2581 caattcttta tatccgataa tcgattctgg gatcgtcaaa tcaggtaaaa aatgctgatt
     2641 tgtatgatac ttattgattt catcaatctg ttcaggtaca tttccccaaa tacggacatc
     2701 atgcccgttt tctgccaatg tttgtgctaa agcagtgccc caagatccag gaccaagaac
     2761 tgcgattttt tgtttcatgg atattcctcc aaataaataa ttgataattt ccattatagt
     2821 atacatttct taaagttggg actaatttcc attgttttta agtgtttgtt tacttctttt
     2881 atcgttttta cttgaaataa tcaatgatat gttacaatag tctacatttt atttttacac
     2941 tcgttatgct ttgcttgttc ctaagaggaa gcagttttga caatgttact accagcatta
     3001 ctattaggag gttaggaaac ttctcctagt gagtattgat ttaaaataca taagagacag
     3061 attgaacatt tctaaatctg tcaggtcctg caacggacag attgtctctg ttgacggttg
     3121 acataggaac ttttgtttta gtcaacattc tggatacaaa acgctatgta aactggcgca
     3181 actcgttgta cttggacttt tactacggat ccatgcaaca actaaatagt actattgcat
     3241 aacaaaaaat ggttggcacc cacatgtccc attaaacaga ttataagttg aactgtagat
     3301 aatgcaaaaa atgtgtttta taacgtaatt gatgccaata ttttaaaaaa ataaagggag
     3361 cagttatcgc tgctctcttt attttttata ttctttttat ccagtctatc ctaataatca
     3421 aactgacgat agtagcggcg tatctctaat ggatggcagt tcaactgctc cacatgagca
     3481 tcgatgcgtt gtgtcactac atgcatcaat aagaaagaca aaacacgtcc gcgatatttt
     3541 tcactgatac cttctactgg atagtctttt gtgtctatca atgtataatt gctgcaaatt
     3601 tttggcaaaa gtttagccac acgttcagat aacactctct gttcatcttc gcctaaaaat
     3661 aatgtgactg gggttgtttc ttctacgatc tctaacgtcc cgtgaagaaa ctcggcagca
     3721 tggatagatt tcgatggaag ccagctttgt tcttcccagt aacacatggc atatgaataa
     3781 acagctcccc attgattccc tgctccaata aaataatgca tactatcacg tcgatgtttt
     3841 tctgcaaatg ataagccaaa tgcatctgct tttttctctg cttccactaa ccctgttggt
     3901 aaataacgtt cgagctgttc atagtattct gaataagctt cgaattctcc attcaaatac
     3961 atcaatcgat ctgctgccat aaaaaatttg atttgttctg ttccaggagc tggataagtg
     4021 accacaaaat cgcatagctg agacaactta ctgttcgctt tatcgataaa tccaatcagt
     4081 gtcgctccta ctttcttgat ttgttctact gctttcacta cttcttgagt tgtaccggta
     4141 acagaggaca aaataacgat agaatctttt gttaatcttt tgtttccagt agtgtaatat
     4201 tctgcagcat gctgtacaaa gacaggcaag ttgcttttcc cattcatata agtgacagct
     4261 tgcatcgagg aagcatacgt tccaccgatt cctagataat agacagcgtc aaatttttcc
     4321 gcataaagtt tatcgatgat ttcttctact tgcggacgta aagctagtgc gccttttatt
     4381 gctttgattt gttcttcttc attgaatttc agcatgattt ccacctctta taatttactt
     4441 ttcaagactg tatcaaagtc ttcaaaaatc tttttgttca agtcaatacc tttagctact
     4501 gcggttttat aagcgaatag ttgaaacgga acgacaagcg ctaatacaga cagatattca
     4561 tcacattcta ttgctaatcc aagtgttttc tcgtttcttg ctttttttgt ggtaatggtg
     4621 agagtctctc caacgtaccg actcatatat ctttgcaact tttgcgaacg ttccttattt
     4681 acactttccc cttcaataaa gaatagcaaa tgcgaagcat ctgcctccag gtatgggccg
     4741 tgcatataag cttctaactc aaatccttgt gacggaacgc ggattgtttc agtcaatttc
     4801 gtctctgctt ctttagctgt tccccagttt ggaccatatc caatggtgac gaaacgctgt
     4861 gctaaacgaa ataaactttg atatccatca aaaaattctt ccgttttttg aataatcgct
     4921 ggtaaatggt tgattatttt gcgcaactgt tgcatgtagt cttgttctat ttttttgctg
     4981 attttatttt tacttattcc aattcctatc cctaagagta gtaactgtaa aacagtcgca
     5041 gaatagcctt ttgtaacgaa gcctactgat tctaccccgg cacctaaatc aataatttgt
     5101 gcagcttcta aagcaagtgg actttgcacg tcatttgtga tagcaacaac aggtaaccct
     5161 tgtttttgta tcatttttac tgcttcaaca gtagaagcac ttttcccgct ttgtgacaca
     5221 gcaatcacag tatcgattga aggagaaagt tttccataat tgaaaaaata aaaaggttct
     5281 tgaatatcga cagtaagatc accataagat tccaatgctg gcttcgctgc taaacaagcg
     5341 ttataagatg aacccgttgc taagatcagt aaattcactg tttttttcat atgttctctt
     5401 gtcgaaaaat cattcttttt caagatcgta cttagtactt ccttttcttc atagatataa
     5461 tcttgcatcg taaacatttt ctctgctcct tttttaaaaa atccctatcc acgaaccaaa
     5521 aattccaaag aaagctagtc cgatcaagat cgttaatggt ttcacttctt tcttcaatag
     5581 ataaaacact atgccaaatg ctccaagaca taagatattt ggaacgatat catcaaaaat
     5641 actttgaaca gtcacagcat tttctcctga accgattttt aaaggaatat tgatggtgat
     5701 catcgtggca gtcattgctc caacgaccat cagtccaata atagacgcgc ctaatgtcaa
     5761 actttccatc ataccgtttg cctggatctt tttcaaaaaa cccgttccta agcgatatcc
     5821 ccaacctgtt gctagatagc gaaacagaat gtgcggaata ttgaaaatca aaatgaacaa
     5881 gatagggcct aaaatatttc cttgcatggc taaagaggta cccacacctg tggcaatcaa
     5941 tcgcaaagtt ccccaaaaga aagaatctcc taaccctgca agaggtccca tcaaagatgt
     6001 tttgatacta tcgatcgcag tcacatcaaa ttcaggatca tccttatttt cttcctccat
     6061 tgcagccgtg attcctaaaa tcagtgtaac gatatgtggg gtcgtgttaa aaaactctag
     6121 atgccgtttc aaagctgctg ccatttcttc tttcgactga tataactttt tcaaaatcgg
     6181 aatcattgca taaacataag ccatattcat ctgacgttca taattccaag aaaactccat
     6241 ttgaaaagaa cgccaaaata cgtgatttag ctcttttttc gttattttgc tgtttttgat
     6301 ctcagaattc ttcatcttct tctacctccc ctgcagttgt ttgaagtggt agttgacgga
     6361 cattcataat attgactacc acaactgcta aaatcgcacc aaaaatagct actccggtca
     6421 caggaatctc taagtaagaa gctatagcaa aacctaagaa aaaatatggt gcaacttgtt
     6481 tgttgatcag caaacgagca agcatcgcaa aaccaagagc tggaatcaac cctgtagcta
     6541 ctgacaaccc cttttgaacg aagtcaggaa tcatatttaa tagatttccg atcgtattgc
     6601 ttcccaccat ataagaaacc attaccacaa caccaagcat caaggaaaga ccgataccgg
     6661 caagcaagtg catatgctcg actcctttat aatttccttc ttctgcataa ttgtctgctt
     6721 tatggttcat aattggaatc aaaagcccta agtaaatatt tttcaatatc aatgtcaacg
     6781 tagcaatcgg caaacctagt aaaagcgcgg tttctgtacc ggctccagct gtaatagcaa
     6841 aagccgtccc taaaatccca ccggtcacaa catcaggtgg aatcgaagca cctactgaga
     6901 acgatccgat aaatgctaac tctaaagttg cgcccataat gatcccagtt tttacatcac
     6961 ctaacacgat tcctgtaaac agccccgtca caatcggacg agaaagcaac gatgtcccta
     7021 gtgcatattc tgattgtgcg acaaatgcaa caattcctaa taagactgct tgtacaatca
     7081 ttgaggttct tcctttctta caaaacttct cttacatcca cttttttatc tgctggtact
     7141 tgacggattt ctacttctat ttgtttgtca attaatcgtt tcaagcgttc ctcctcttct
     7201 ggtaaaatat ttaccgcttt tccgatgctg cgagtaccag gttttgcctt gatccctcct
     7261 aagttgatgg ttttgatttc tggatacgct tctgccagct tatatgcatc ctcgatcgat
     7321 tccactacga tgaataactt atatttatct gtcaccccgc ttttcaacgc ggcaatcgag
     7381 tcatcaattg atttgatcac tagtttgact ccttgtggct tggctaattt gatcgttgtc
     7441 tttcgtatct cattcgtagg tacatcatca ttagcaatca aaatacagtc tgcgcctatt
     7501 gtttgtgtcc atgaaaaagc tacttgtccg tgtaataacc gatggtcgac tcttgtcaat
     7561 acaatcattt ttcttcctcc taaaattcgt cttcttctat tttctgattg agccgctcgt
     7621 tacaaaactg gatcattttt tttgaatcag ttagcacctg ctggatcaaa acagttgtct
     7681 ctacttcagc gtccaaccga gatactagct caatcaatag tggcaaattc aatccggcaa
     7741 cgagatagac atgatcccga ttgatatatt gtaagaattc gttgttgaca cttccgccaa
     7801 aaagatccgt cagaacaatg atttctttat ctttgtacct cataaaaagc tgccttactg
     7861 tttccgctaa atcaaaattc tcatcagtat agcaatcgaa tacagtaata gaatgatttt
     7921 tcccatagat cagctctaat gaatctaata tccctgaagc aaattttcca tgggaagcta
     7981 agataatttc tctatccact ttttcatctc ctcttttcac ttacacatac taaaaaagca
     8041 agttgcatgc caaaattagt ggcaacaact tgcttatgtt caaaattctt cttctatcga
     8101 gagaaactct gtatcttcac tcaaaatatc atagatatag gcaatctcag aattggggat
     8161 catgacacta taatgctctt caatgacact aaacgcagat ttaatagcag ataacccttc
     8221 tttttgacac tgcttccatt tctctaatcc ttgataggtt tcgattggca tttgtcggat
     8281 caatcgttca atgagacagc tcacatggac atacaacgct aattttttgg gattgctgat
     8341 gcttcgattc aactgcacct ctaattctct cataaaatga tcgatttctc ccatcacctt
     8401 ctctgtatct agaatagtca cggaatccag tactttttcc aatgaaaaat tacgaatgat
     8461 gcgctgattg aatatttcct tctctgaagc attcatagta gtgtccaacc attcagctag
     8521 tacagacatc ttttcccccg agatgaggtc ttctaatgaa agatagtcga tattggtgat
     8581 atcaggatct gtggtgccaa tcattcctac gacttcgtat atggaaaata atgggtcact
     8641 gtttttctta tcttttaatt gctgatattc atatgggatg attttcaact gacaagccgt
     8701 aggtaagctt ttttctagca gttcacttaa gtggaccgcc gtaccgattc cagtgaagca
     8761 agtagtaatg agtgctttgg gtttgttttc ctctggatag agcagtcgcc attctggctg
     8821 gatctgtcga acagtctctt ctgctatctc ttctaatgat acattttttt gcaggcattc
     8881 ccctactgtg attgctaaag gtgtcgtcac attattcaaa atcaaaagcg gtacggataa
     8941 ctgtttttta aaaaactgat ggatttcttt cagagatccc atgtctacta atatgatcaa
     9001 tccatttgaa acatcgctgt tttcgcaata atcaaggatt tctgaagcaa tctgctgtgg
     9061 tgtgacatct agtggcatat caaatgattc aaatagatcc tcttgtaata aacgatttac
     9121 tacatttgcc atactgctag cagttgcata accgtgagca gcaatcatcg cttttggcag
     9181 tcgtttttct tttgaccatt tcgctttttt tagatagatc gtcagtaaaa tccgatccat
     9241 acttgtgtct tcaatatcta atttgatctt acttaattcc agaattcgtt ggacatagtg
     9301 ataactagat ggataagact gagaaacttg ttgttctaat tctttgatta gttggataat
     9361 atcatgttct tccggtaacc atttgatagt gcttcgctga tagagatagt aactcaaggc
     9421 atagacactg tttccatcga atttgatttg ataggcagtt tccatttgtc gaaaggtctc
     9481 tcggatagat tgtgtccaat aaaccagcag ctcgtgtttt tcttgtctac tggtctcaaa
     9541 cagcaaaaaa tcaaataatt gaatcacttc ttgctttaat tttccttcgc aagaatatag
     9601 cagacagtta tttttcttga aatcaattag gatttgctca aaacttttaa cgattcgctg
     9661 ctgttcgggc tgttgcttct ccattaattg ctctaaggtc tggtcttgcg taatatagat
     9721 tccttgctgt gctgtatttt tagtaatagg ctgcgcaagt agttcttttt gcaaatgatg
     9781 gatcgtgaca tggatttcag gctgttcacg ctgttcagca tatgcttttg ctgctgaaac
     9841 tttgacgata tttttcagct ctcctatgtt tccggataat ttctgacttg ttaaaagtga
     9901 caaagctggt ccactgactg acaaagggct ccccattttt ctttgttctt cgagaaaaaa
     9961 agagtaaatc agttctagcc gttcattacg actacgctga ttaagtgcag gtagttcgat
    10021 tcggataggt attcttcgaa tgaaagttgt taagaaatga ctagtaatct cttcagtcgt
    10081 agcaaaaaag agacgtgttt tgatccggcg tgacaaatta ggttctccca tacgataaat
    10141 cacaccttga tcaagatagg tgaacagttt ttcctgacct tctggtgtta agcgatgtac
    10201 ctcgtctaaa aacaagatcc caccatctgc tgcttcaaaa gccccttttt tattttcttc
    10261 tgcaccagtg tacgctccct taacatgtcc aaacaaattg ctagtcaaaa gttcaggatt
    10321 atctgcatat tgtgcacaat taaaagtgat gaatggtgca gaatcttcta gcagatcatg
    10381 aagcagacaa tactgataga ccaaattaac taaataactt ttacctgtgc cactttcgcc
    10441 tgtaatcaaa agtggtaatc cgccatctgg atagtaaaga gctgttttga tttgttcgat
    10501 actttctttc aaactggcgt cgtatccgat cataaaagag aataagtctt ttggttgcgc
    10561 gaaaaaaggc tgttcctctt tgagatcatc gatagaaaaa taaaaattcg tgcgtaactt
    10621 aaaaaattgt ttttcaaaag cttctttgtg gaaataaatc actggtcgag aattgatttt
    10681 gactagtagt tgctcatcat ttaactggtt caaataatga ctgactgtgt ttctttttac
    10741 cccaaaatga tcagctaacc tttgtgctgt aaagacctcg ttcatatgtt ctaaatcgaa
    10801 aaaggctgtc tggttctcta agtaacataa aatttccttc ttcaacaact tattcacccc
    10861 tcctccatca tagaaaacgg attcattttg tcaataaaat ctttaataaa gaattgaaaa
    10921 atatatctta ttatagacag cagcctactt atcccttata atttttattc taaaaaacgt
    10981 acagttcctt ccagttttta tactggaatt tcaacatatg aaagaataag gaggctatga
    11041 ctattcaatg aaatcggata aatataaaaa caggaaacat acttcctgga aaacttttcg
    11101 agaaatcatc ggatcttatc tcgctgtagt tgtatttttg cctttgcttt atatggctat
    11161 gacagaacaa actcgtttta ttggcgtaga tacgcaatat atagccgaaa caggattttt
    11221 attcatcttt atcttgattt ttgcgttcta taaatccttc catacaaaat aagattcaca
    11281 gatttcaact ttcctgtctg tttttagcta ttacaaatcc ttatatgtgg tataacaaca
    11341 atttattggc ataatgagga aaaacgagtt atgatgcttt cagagagtaa agaggtactg
    11401 ttgtcaccat cctttttatt gattacgtac accactctta gttttttccc attgattacg
    11461 aaaacaaggt agaaacattg atgttcctac cttgttttct ttttttattt tcagatcaaa
    11521 atctcaagtg gcttttgact tgatgtcggg aggttgtctt cgttccatca aacctgatag
    11581 aatctgcgcc aaaccatagt tttctccact attatttatt cctctaagat agtacccaca
    11641 ataaacgaat aaatattttt ctcattttca tactccttct ttgaaaatca attagatttc
    11701 tctcaaccca cttttttaca aaaattacgt agcaaaaaag attgcaagaa caaattaaaa
    11761 aagaagtaa
//
LOCUS       DACCWV010000089        11623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCWV010000089 DACCWV010000000
VERSION     DACCWV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.090"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IVW84_001991"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IVW84_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7918176.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IVW84_001992"
     CDS             complement(323..892)
                     /locus_tag="IVW84_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7918177.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IVW84_001993"
     CDS             1078..1980
                     /locus_tag="IVW84_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7918178.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IVW84_001994"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IVW84_001994"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7918179.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IVW84_001995"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IVW84_001995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7918180.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IVW84_001996"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IVW84_001996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7918181.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="IVW84_001997"
     CDS             8548..8733
                     /locus_tag="IVW84_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7918182.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="IVW84_001998"
     CDS             complement(8795..9991)
                     /locus_tag="IVW84_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7918183.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="IVW84_001999"
     CDS             complement(10161..11597)
                     /locus_tag="IVW84_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7918184.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3423 a         2482 c         2041 g         3677 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttt
//
LOCUS       DACCWV010000090        11275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCWV010000090 DACCWV010000000
VERSION     DACCWV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.091"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..305
                     /locus_tag="IVW84_002000"
     CDS             6..305
                     /locus_tag="IVW84_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918185.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVW84_002001"
     CDS             378..1739
                     /locus_tag="IVW84_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7918186.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVW84_002002"
     CDS             1761..2021
                     /locus_tag="IVW84_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918187.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVW84_002003"
     CDS             2035..2787
                     /locus_tag="IVW84_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7918188.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVW84_002004"
     CDS             complement(2881..3486)
                     /locus_tag="IVW84_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7918189.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVW84_002005"
     CDS             complement(3539..4513)
                     /locus_tag="IVW84_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7918190.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVW84_002006"
     CDS             complement(4891..5670)
                     /locus_tag="IVW84_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7918191.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVW84_002007"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVW84_002007"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7918192.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVW84_002008"
     CDS             7161..8558
                     /locus_tag="IVW84_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7918193.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVW84_002009"
     CDS             8639..9079
                     /locus_tag="IVW84_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7918194.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVW84_002010"
     CDS             9227..9553
                     /locus_tag="IVW84_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7918195.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVW84_002011"
     CDS             9554..10303
                     /locus_tag="IVW84_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918196.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVW84_002012"
     CDS             complement(10383..10838)
                     /locus_tag="IVW84_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7918197.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3582 a         1964 c         2308 g         3421 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgct
//
LOCUS       DACCWV010000091        11128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCWV010000091 DACCWV010000000
VERSION     DACCWV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.092"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="IVW84_002013"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="IVW84_002013"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7918198.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="IVW84_002014"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="IVW84_002014"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7918199.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="IVW84_002015"
     CDS             2139..3020
                     /locus_tag="IVW84_002015"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7918200.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="IVW84_002016"
     CDS             3017..3733
                     /locus_tag="IVW84_002016"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7918201.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="IVW84_002017"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="IVW84_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7918202.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="IVW84_002018"
     CDS             4450..5451
                     /locus_tag="IVW84_002018"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7918203.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="IVW84_002019"
     CDS             5448..6809
                     /locus_tag="IVW84_002019"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7918204.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="IVW84_002020"
     CDS             6769..7920
                     /locus_tag="IVW84_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7918205.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="IVW84_002021"
     CDS             7895..9064
                     /locus_tag="IVW84_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7918206.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="IVW84_002022"
     CDS             9239..10618
                     /locus_tag="IVW84_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7918207.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="IVW84_002023"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="IVW84_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2494 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DACCWV010000092        11073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCWV010000092 DACCWV010000000
VERSION     DACCWV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.093"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(291..659)
                     /locus_tag="IVW84_002024"
     CDS             complement(291..659)
                     /locus_tag="IVW84_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7918208.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            809..1291
                     /locus_tag="IVW84_002025"
     CDS             809..1291
                     /locus_tag="IVW84_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7918209.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1328..2338
                     /locus_tag="IVW84_002026"
     CDS             1328..2338
                     /locus_tag="IVW84_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7918210.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2453..3166)
                     /locus_tag="IVW84_002027"
     CDS             complement(2453..3166)
                     /locus_tag="IVW84_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918211.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3356..3946)
                     /locus_tag="IVW84_002028"
     CDS             complement(3356..3946)
                     /locus_tag="IVW84_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7918212.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4430..4657
                     /locus_tag="IVW84_002029"
     CDS             4430..4657
                     /locus_tag="IVW84_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7918213.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4673..5839
                     /locus_tag="IVW84_002030"
     CDS             4673..5839
                     /locus_tag="IVW84_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7918214.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6073..7116
                     /gene="hrcA"
                     /locus_tag="IVW84_002031"
     CDS             6073..7116
                     /gene="hrcA"
                     /locus_tag="IVW84_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7918215.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7210..7773
                     /gene="grpE"
                     /locus_tag="IVW84_002032"
     CDS             7210..7773
                     /gene="grpE"
                     /locus_tag="IVW84_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7918216.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7824..9653
                     /gene="dnaK"
                     /locus_tag="IVW84_002033"
     CDS             7824..9653
                     /gene="dnaK"
                     /locus_tag="IVW84_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7918217.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9804..10970
                     /gene="dnaJ"
                     /locus_tag="IVW84_002034"
     CDS             9804..10970
                     /gene="dnaJ"
                     /locus_tag="IVW84_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7918218.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3776 a         1866 c         2323 g         3108 t
ORIGIN      
        1 cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat tttttcctta
       61 ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtaga aaattcaaag
      121 ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat agcagatact
      181 ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca aaacctgtat
      241 aaggtgatga atagtttcct cgccagtaaa aggtttattc gtataaagct ttatcgccaa
      301 aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc aatcaattcc
      361 cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac gtagcctgct
      421 tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga
      481 tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat aggttggttc
      541 cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa aaggtctttg
      601 atcttttgtt gatgttcttt ttctttgatt tgattgatga tcggctgaaa tttttccatt
      661 ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata gttatgtata
      721 aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata agatgtctca
      781 aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat tcttacacga
      841 aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag atgctttggt
      901 aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct taaaagataa
      961 aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt tcaaagcatt
     1021 agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg aagaacaaac
     1081 gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc cgattattat
     1141 tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac cagatcaaac
     1201 gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga tgccgatatt
     1261 tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata cttggaggtg
     1321 aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg ttactgcacc
     1381 atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa agagaatgga
     1441 agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata aggaaaaatc
     1501 aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg aaaatatcca
     1561 aatgatcttt ccgacttggg gaggagaatt attgattgag attctcgatt ttgtcgattt
     1621 tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt tgctacttgc
     1681 tgtcacatta aaaacaggga tagccactgc acatggaact aaccttattg atttaagagg
     1741 agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga
     1801 atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg tggccccatg agcattcatc
     1861 atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt
     1921 aaggatgaag ggacgattat taggtggttg tattgatacg atcaggcatt taatcggtac
     1981 tccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc cgatcttgtg
     2041 gtttctagaa aactgtgagc tatcgtcagt tgatttaaga agatcacttg ttcagatgaa
     2101 actggcaggc tggtttgatg gcagctcggg gatcatgttc ggtagaagtg cggcagagga
     2161 tttgttagat tataaaatga aggatgtata cgaagatctg tcaaaggaat taaaactacc
     2221 gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga ttaatggtgc
     2281 ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa
     2341 aattttttcc tgccttggag tctatcaaag accaataaat ctaaatagaa aagaaagtga
     2401 aagaagctga caaaatgctg tcagcttctt tttgtataac atattagaaa gtctaattat
     2461 ctataactgt cacatcgatt aactgtttca cgtcattggg atttcctgta ggaatagcta
     2521 ctacagatgc attatcaatg atttcttgag ttgtttctct aatgaacgct ggataagatg
     2581 tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt gtcccgtcat
     2641 ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc ataaccacat
     2701 ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct gcaggataat
     2761 gaataacatg taattcgaaa tcacccgatg tatctctaaa agtattacct ctaatagcta
     2821 gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga tctattgact
     2881 gagaagactg tgaggaggtt gtgtctattg ttgaagaaga aatatttgtc tcaactgtac
     2941 tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa gatgtattcg
     3001 tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt tgtctgattg
     3061 aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac gtacatccta
     3121 caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa aatacctcat
     3181 tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa accttgtcta
     3241 tgaatataaa tagagtgtat cttctttgat aaatgtcaga aaaagtgtga caaataatca
     3301 tcagtaaaat tacttgtcac actattgata aggagtattt aattgaagag aatgattagg
     3361 agaaaggatc tccgtacttt ttatagtaat catagaatcc aagtttgtat agacgaatcg
     3421 ttttttctct tatttcttct gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt
     3481 ctataggagc tgttccattc gcagtaatca aattacgatc tttcacagct tgttgttcta
     3541 taaaatcttc tttattctga taattttccg attctaacca aggaaattgc gcatttccgg
     3601 tgtgtttgta accttgcaat aatccgctct gagctaaata atcaactgcc ccacaaatag
     3661 cagctactgg tacgtctgtt tttaacaatt tatcgattat ttgatataaa tgatgatttt
     3721 ttatgtccca tgaattgcca ccgatcataa taaaaaggtc aactttttca ggtatttgtt
     3781 cgattaagta atcgattttt gtttttaatc ctccaatgga agtgacctct gattggatag
     3841 aagcagtttt gttttgccat ttctcatttt ggtttagtgt gctagtaata taagcacctt
     3901 cccaatcggc atattcctct aatagaaacg taatcgcagt tttcattttc aagctctcct
     3961 ttactatcta taacaaggac aacagtttta acctacagac attcattaaa attgggtagc
     4021 gacagcgaaa acaacaattc ttaagtttta gacagagtat acagatagtc aacttaatta
     4081 aatacgaact aaataaaaat gacaggtaat catttttgtt taccagcatt ataacatttt
     4141 gcatacatgg atgacaaaag aaccaattcc cacttgcttg agtacaatat taaaagaaac
     4201 tggaaggcaa aaagtaatta tagaaacgaa aaataaatca tatcgggcac tcagacatga
     4261 aaacaaccct agagatatat aactaggtaa ccagtttaat gaaagaaaaa atcatccaag
     4321 aagtcgattc ttggattttt tagtggattt gttcgccaaa tacgtttagt atgcgtatat
     4381 gagaggttgt aagcaaagcg ttgtggtctg agcacttaag gaggaatctg tgaaaatagc
     4441 ttttcatatt tttgcagatt ttgacttaat gccgaaggtc agctttgcga ttaccgttta
     4501 tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat ttttagagct
     4561 aggtttcctt gattctaaat atgcggctta tttcaatgag ttggtgcttg aacgcgattt
     4621 taagaaggtc gaaaacaggc acgacttttc gacgtaagga gaaagaattt caatgataga
     4681 aacaataaat cccgcagaaa acaaggtttc tgcgtacatt catatcccat tttgcgagca
     4741 tatctgctat tactgtgatt ttaataaagt ttttttagaa gggcagccag tagatgagta
     4801 tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa agtcatccga cagcgaattt
     4861 agaaacactt tacgtagggg gcgggacacc aacttcttta tctgctaaac aacttgatcg
     4921 actgttatca ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga
     4981 agcgaatcca ggtgatttga cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa
     5041 tcgtttatcc atgggagtgc agacatttga taatcgctta ttgaaaaaaa ttggacgtaa
     5101 acacaccgca gaagatgtgt atcaaactat gcaattttta gaagcagaaa attttaccaa
     5161 tgtaagcatc gatttgattt atgcattacc gggacaaaca ctagaaggat atctggatac
     5221 tttggaaaaa gctttggctt tggatctgcc tcactattct ttgtactcat tgatcctcga
     5281 aaacaaaaca atgttcatga actgggtacg acaaggaaga ctcgaactgc ctgatcagga
     5341 aacggaaacc agaatgtttg aagagacgat tgaagcaatg gaaaagcatg gacgtcacca
     5401 atatgaaata agtaactttg ggctctccgg acatgaatct caacataacc tcatgtactg
     5461 gaacaatgcc cactattttg ggtttggagc tggtgctagc ggttatcttg gagatattcg
     5521 ttaccgcaac aaaggaccaa tccaacacta tttacgccca ttacgagcaa atgaacgccc
     5581 ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg
     5641 actaagaaaa aaaacaggga ttactaaaaa acatttctat gatcgctatc aaaaaacaat
     5701 tgaatcggtg tacggtgatg tgctaaggga attagagcaa gaagggttac ttgttaatga
     5761 agaagaccgt gtttatttga caccaagagg gacctttttg gggaacaatg tgtttgagcg
     5821 ctttttgttt gaaaaatagt ttaaagaata tttgtttttt atctgaattt aaataaaaaa
     5881 agttcaaaaa tacaataaag gactattgaa acgttgatat aactggattt acatcaatta
     5941 tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct cttgacagga
     6001 gaagggcttc ttgctatatt tataagtgga ttagcactta gaatatatga gtgctaatga
     6061 gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat catccaacac
     6121 tatacgagta gtggcgtgcc cgttggttcc aaaacattga tgtctgaggg agtcaaggcc
     6181 agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt gctgttgaag
     6241 actcactctt cttctgggcg gattccatct gctttaggct atcgttatta cgtggatcat
     6301 ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca gtctctaggc
     6361 agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt atctgagctg
     6421 acaagctata cgactttttc attgggacct gaaataaaag accgccgact gactggcttt
     6481 cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga tatggggaat
     6541 gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg
     6601 gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta ccataaactt
     6661 cgtacggaga ttccgatgat tcttcataaa tatttccaaa cgacagaagg gatgtctcag
     6721 ttatttgaca ccgttcttgg tcatgttttt gaagataagg tttatgtcag tggacaaatg
     6781 aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat gttttctttt
     6841 atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa tattcatatc
     6901 aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat tcaagcaagt
     6961 tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac tagtatgcct
     7021 tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc taaacttgct
     7081 gattactacc gttcgcttga cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag
     7141 ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata atgaaaggaa
     7201 gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga tgctcagcca
     7261 gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc tgaagcggaa
     7321 gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa atacttgcgg
     7381 gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa
     7441 aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa cctggaacgt
     7501 gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg
     7561 gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa
     7621 ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc gactgaagat
     7681 acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca tgatcgcgtg
     7741 ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg gatataaaac ttacactttc
     7801 aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta ggtacgacta
     7861 actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa
     7921 accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta ggggaagttg
     7981 caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc catatcggtg
     8041 aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa atctcagcaa
     8101 tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag
     8161 cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca aaagatgctg
     8221 gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca gctgctcttg
     8281 cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac cttggtggcg
     8341 gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta ttgtcaacag
     8401 ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac tacatggtag
     8461 cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct ttacaacgct
     8521 tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc acacaaatca
     8581 gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg acattgacac
     8641 gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta ccagtacgtc
     8701 aagcgctaaa agatgcaggt ttatcacaat cagaaatcga cgaagttatt ttagtcggtg
     8761 gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga aaagaaccaa
     8821 acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga
     8881 tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca ttaggtatcg
     8941 aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt ccaacaagta
     9001 aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc catgtattac
     9061 aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa ttgacagata
     9121 ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt gataaaaacg
     9181 ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa atcacgatca
     9241 aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtaaaagat gcagaagcaa
     9301 atgcagaagc agataaagct cgtaaagagg aagttgacct acgtaatgat gtagatgcat
     9361 tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac gaagaagaag
     9421 tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc tgttgaagca aacaacatcg
     9481 aagaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg actgttaaac
     9541 tatacgaaca agcggcacaa caacaagctc aagaaaaccc agaagcagct caaggcggag
     9601 cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa taatcagttc
     9661 actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga aaaatagttt
     9721 gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg tgaaaatgat
     9781 gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa gtcttagggc
     9841 tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat
     9901 accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa gtatcagaag
     9961 catatgagat tttaagtgac ccgcaaaaac gcgctgcata tgaccaatac gggcatgcag
    10021 gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga ttcggcggct
    10081 tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt ggaggaggcg
    10141 gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat acggtcgact
    10201 tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat cgggaagaaa
    10261 tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac acaaccaact acttgtcata
    10321 aatgtcatgg tgcaggtacg attaatgtag aacgccaaac accacttgga cgagtgatga
    10381 gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga aatcaaagaa ccttgtccaa
    10441 cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat gtaccagctg
    10501 gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga gaaaacggcg
    10561 gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga aagtgatatc tttgatcgtg
    10621 aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct ttgggggatg
    10681 aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct ggaacacaga
    10741 caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag
    10801 gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa caacaaagag
    10861 aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag caagaagacg
    10921 gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag tttttctagt
    10981 aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatatgtt cgcctggttt
    11041 ttttgtagtt gtcatgtttg taccaacaaa aaa
//
LOCUS       DACCWV010000093        10617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCWV010000093 DACCWV010000000
VERSION     DACCWV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.094"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            288..2234
                     /locus_tag="IVW84_002035"
     CDS             288..2234
                     /locus_tag="IVW84_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7918219.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2237..2692
                     /locus_tag="IVW84_002036"
     CDS             2237..2692
                     /locus_tag="IVW84_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7918220.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2706..4034
                     /locus_tag="IVW84_002037"
     CDS             2706..4034
                     /locus_tag="IVW84_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7918221.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4068..4352
                     /locus_tag="IVW84_002038"
     CDS             4068..4352
                     /locus_tag="IVW84_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918222.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4354..4869
                     /locus_tag="IVW84_002039"
     CDS             4354..4869
                     /locus_tag="IVW84_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP7918223.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4885..5547
                     /gene="rpe"
                     /locus_tag="IVW84_002040"
     CDS             4885..5547
                     /gene="rpe"
                     /locus_tag="IVW84_002040"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7918224.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5554..6126
                     /locus_tag="IVW84_002041"
     CDS             5554..6126
                     /locus_tag="IVW84_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7918225.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6313..7833)
                     /locus_tag="IVW84_002042"
     CDS             complement(6313..7833)
                     /locus_tag="IVW84_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7918226.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8076..8261)
                     /locus_tag="IVW84_002043"
     CDS             complement(8076..8261)
                     /locus_tag="IVW84_002043"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP7918227.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8245..8427)
                     /locus_tag="IVW84_002044"
     CDS             complement(8245..8427)
                     /locus_tag="IVW84_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918228.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8668..8886
                     /locus_tag="IVW84_002045"
                     /pseudo
     CDS             8668..8886
                     /locus_tag="IVW84_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9381..9980
                     /locus_tag="IVW84_002046"
     CDS             9381..9980
                     /locus_tag="IVW84_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7918229.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10044..>10617
                     /locus_tag="IVW84_002047"
     CDS             10044..>10617
                     /locus_tag="IVW84_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918230.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3510 a         1671 c         1982 g         3454 t
ORIGIN      
        1 tataataaaa aaatacgaat tttagtgacg ccattatatt gtttttattt ttcaattgta
       61 atatttcata ttacttccat aataaggagt attttctatt ggaatatctt agctctttta
      121 aggatttctc cttttttgtt tctcattttt taagattatt tggatttcct ttacaacaaa
      181 cttccacaag tattattgac atagatgtaa ttgagggaag ggggttccag tgctagaata
      241 aataatgaaa agaggtaaca aatgctagcg ttaagaggtg aacaagtttg attgaaagac
      301 atgtaaattt attaaaatta ttaagtcgac aagaagactt taaacccgct tcttttttta
      361 gtgataaatt ggcagtgtcg accaagacca tttatcaaga tattgaattg ttggaagatt
      421 atgtagttaa ttaccaagta acaattgagc gattaccttc tttgggtatc aaactcatag
      481 gtgataggaa aaacgttgaa catttgattt catcggtaga aaattatctt gaaaaagata
      541 tttattctcc agaaaaaaga cgtgtcgaga tcattaaaga agttttgttt ggtgataagc
      601 ctgtttcttt aactcatttg tctcaaagtt ttatggttag taaaacatcg ttatacaatg
      661 acctgagaat tatcaataaa attattggca gtggagaagt taagttgcag tcatctgcag
      721 aaggaattag ttttactggt aatgaacaag caattcaacg agcaatcaag caacttgtat
      781 tctattatgc gaatgacatg gaatcaaaaa cattttttca agttttaaat atgttgtttg
      841 attcaaaagt tattagtgtc attcatgatt tgctattcaa tgattacagt gaactgactg
      901 aaaaggtttc tgattactat gttcggtcat tgatttctac tttagttatc caatgtaacc
      961 gtttactgaa aggtcatcat ttaactgttg aagaagaatt cctcttcaat agcattcgct
     1021 atatggaaac gtacattgtg gcaaatggtt taattgaaga attgaaggca aaattgaatc
     1081 ttccatttta tgacgcagat aaagaatatc tatgtcgtca actatttgca catcgtgtga
     1141 caaataattt aaaaaccagc aatgaagagt acgctatgag cgttcataaa ttgattgaaa
     1201 gaatgagtga gattgaaaaa attgatttga ctcaaaatga tcgattatat caatccttat
     1261 tgtatcatgt gccagcaatg gtccttcgtt taaaaaaagg tattaaaatt caaaatccgt
     1321 tattagaaaa tatcaaatct caatattcag aactctttag catagtatgg tacgccatgt
     1381 ccatccttga agaagaattt gatgtgactt taacggacga tgaagtttcc ttaattttga
     1441 ttcattttca gattgctatt gaagatcagt ccaaagcaaa taatatcatc attatttgcc
     1501 aatacggtat ttcttctgcg caattaattt acaataaagt tcgacggttt ttaccagcaa
     1561 aagataatgt agaaatatca actttagata agtttcattt agctgaaatt ggggaagttg
     1621 atttgattat ttcctctctt tcctttgatg caggtggtat acccttagta agggtatctc
     1681 cattggtaag taaaacagat tatatgaata ttatggaagc ttatactaaa tatttaatag
     1741 aagaaccttc gcgatttatt caaaatgaac tgattcctaa agcggttaaa gttcccacca
     1801 tatctaaatt tgttcaatca tctttagtaa aaacaggagt acaggtggaa agcaaagaag
     1861 aatgtttgga tttgatgatt tccgaactag aagccaattc attagttact gaagagtttc
     1921 gtgcttctat cttctctaga gagaaaatgg ggaatactaa tttagaaagt ggtgttgctt
     1981 taccgcatgc gaatccatca atggtcattg aatccaacat ttttattgta gcattaaaaa
     2041 atgcgattaa ttggggcgat cgaaaagtca aattagtcat tatggttagt ttagctgaag
     2101 aagatgtatc tgagattaga ggagtatatg aagaacttta ccaatttatt gggcaaaaag
     2161 aaatggtcga tcgcttgtta caagttcatg atagcgaaaa attactaaga atttttcaac
     2221 aaaaaggaga ataaaaatgt atctagataa agaattatgt ttatttcaat tagaagtgaa
     2281 agatcaaaaa gaattgtttc aagtaatgtc tgaacaatta aaaaacgctg gttgcgtgaa
     2341 agatagtttc ttagaaggta tcaccaaccg tgaacaagaa tttccaactg gattagaagt
     2401 aaatcaaatt gggtttgcta tcccacatac agattccgca catgtaaata gctctcaaat
     2461 ctgttttgcc tcattaaagg aaccactggt attttcagat atgacagata aatctcatga
     2521 aatacctgta cgtttagtct ttatgttggc aatgtcacaa cctcatgaac aaattgatac
     2581 actacaaaac ttagttagcc tatttcaaaa tgaagaaaaa gtaaatgaat tacttgcatg
     2641 taccacgaaa gaagcattta ttgagttagt acattctgca ggagtttatt aggaggaata
     2701 gagagatgga tattgtatta aatgcctttg aatggtttat tggattaggg tcaaacgtct
     2761 ttttaccact gattattttt gtaatcggga tattatttgg attaaaacct ggtaaagcat
     2821 ttgtttccgg cgttactgtt ggtatcggtt ctattggttt aggattagtt ttaagtttat
     2881 tatcagatgg tttaggaaca gcaattcaac aaatggggga acaatacggt ggtggcctga
     2941 atatcctaga tattggcgta ggtgttggtg gacctttggc ttactctaca tcattgggaa
     3001 ttttaatgat tccaatttca ttaattctta acttcatatt tgtggttttg ggttggacaa
     3061 aaacattgaa tgttgatatt tggaactttt ggttcgcaat tttcctaggt atgattactc
     3121 aatctgttac gggaaacttt tggtttggct taattggcgc gattgtcgca gtaattttac
     3181 aatggttctt agcagatcgt acacaaaaaa ctgttagcga atttttcggc tatccaggaa
     3241 tctctatcac acatatgatg ggattatcag gagtactatt tgccaaacca gtaaattggg
     3301 tttttgatcg tattcctgga tttaataaaa tagatgcaga tgcagaaaca ttaacaaaac
     3361 gtttcggtgt tttaggtgac acagttgtta ttggcctaat tatcggtatt gtagtgggta
     3421 ttatggcagg ctatgacgta gcaggaattg gaaacttagg aattatgact gcggcagtga
     3481 tgaagattat gcctaaaatg gtagcaatgt ttatggaagg tttaatgcca attgcagaag
     3541 cggcgaaaga atttacgaat aaaaagttaa atggtcgcca agtaaacatt ggaatggatg
     3601 cagcgttaac tgtgggtcat ccaactatta tgtcaacttc tctattgctt gtaccaatct
     3661 cattattgct agcactaatt cttccaggaa ataaagtatt accttttgga gatttagcat
     3721 tctttgcttt cgccatctgt ttaatgattc cttattttaa aggaaatatt gtgagatcat
     3781 tagtaggatg ttcgttgtat gttgttacaa cattatatat ggcaacatgg ttggcaccaa
     3841 ttatcactga tgtgtttaca ttagcaagtt tcgatgtggg gacagcagga ttagtaacta
     3901 tgttgttatg tggattatgg ccagtagggt tgtttgtttt attagcatct tactttggtg
     3961 taattggaat aggtggatta ggagttgttt tagtaggtgc ttctatctac atgaacaaag
     4021 tgaagaaagc ttaatcataa aattaaaata agaaagaaga gatcaatatg aaaaaattat
     4081 tagttatttg tggagcaggt catgctactt ctacaattgc agtagcaaaa ttatcaaaat
     4141 ggttggaaga agaaggatta acagatcaag tgaaaattta tcaatctaaa attgctgatg
     4201 agctaaataa aattgatgaa tatgatgctg tagtaagtac aacaattgtc cctgatgtaa
     4261 ttaaagctaa agtaacgaat ggcttgggct tattaacagg tattggtaca gatgccattt
     4321 atgcagatgt aaaagaaaga ttaggtgttt aatatgcgtt ttcatcatga cactttgact
     4381 attgaaacag tttcagggcg tccttcgtat catgatattc gaaaagaact tttagataca
     4441 gttgatacag caggtattga gaatggaatg ttagtaattt cgagcccgca tacaacttgc
     4501 tcactctttt ttgaagaaac gatgcatgac acaaactttt ttggtgatga ttatttacat
     4561 gtagacatca acaatgtaat ggaaacgatt attccaaaaa tgacgagtga aaatcaatac
     4621 aacagtccgg ggccaaaaca cattgatttt ggaacaagat tatctgatcc caattaccct
     4681 gcagaaaaat gggtcatgct aaataccgat gcacatttac gttcatctat ttatggaacc
     4741 aactcaatga ctttaattgt tcaagaaggt aaacttttat taggagaaat gggccgtgtc
     4801 tattttgtcg attgggatca actaagagag cgaactagaa caatcaatct catgattatg
     4861 ggagaataaa cgggggaata gagaatgaca attgctccat cattgttaaa tgcacctatc
     4921 gcagaagtta aagagttatt tgaaatctta aaagaaaaga atgttgatta tttgcatatt
     4981 gatgtaatgg atggctgttt tgtaccagat atggcttttg gaccaagtgt tattaaagat
     5041 attcgtccac taacagacgt tgtgtttgat gttcatttga tgattgatac accagaaaaa
     5101 attgtcacaa gatttgtaga agcaggagca gatattgtta ccttccacat tgaagctacc
     5161 aaacacgcaa tgaaggtcat tcagttagtc aaggaagcag gagcaaaagt agggattact
     5221 tttaatcctg caacaccagt cagttttatt gaaccagtat tatctttagt cgatcaagta
     5281 ttagtaatga caatcaatcc aggaacagag aataagcatt ttattcaaga gacagtagtt
     5341 aaaatcgaac agttagatgt aatacgcaaa caaaatgatt atacgtatga tattgaagta
     5401 gatggcaaga ttgataatca aacgattaaa gtttgttcta aagcaggggc ggatattttt
     5461 gtatcaggcg gtttcatctt caatgggaaa aataccccgc aagaacaaat tgataaacta
     5521 aatcaatcat tagaggaagt gaagtaaaag atcatgacca ataaagacca aaataaaaac
     5581 tatttagaag cgattatctt agaattacaa aataactcaa aacgtattaa ttctgtagag
     5641 ttagaacaat ttagtgagaa aattgttcaa gctaaacaca tctttctagc tggtgcagga
     5701 cgctctggaa ttgccatgca agcatttacg aatcgcttaa tgcatttagg tttttcagtc
     5761 agccttgtcg gtgaaattag ttcaccacat tcgcaacctg gagatttatt aattatctgt
     5821 tctggctcag gtgaaacagg tagtttaaaa aatttagcgg ccaaagcaaa tcaaagtggt
     5881 gtaagcattg ctttagttac catgaaaaaa gactcgtcga ttggtcaatt agcagattct
     5941 gttcttgtat taccaggtat taccaaagat gaaaatcaac gagaagaaga tactttttca
     6001 caacctatgg gttcaagttt tgaacaatta gcatttctca cctttgatag tttagtgtta
     6061 tctttaatgg ataaaacagg tgaaagctca gaaacgatgt ttgctcgaca tgcagatttt
     6121 gagtagtaga ttttaaagat aaaaaaccat tctctaaatg ctaattattc agcaaatatg
     6181 agaatggttt ttttatttga ttagacataa cttaataatt cagtagtttt gagcgtaagc
     6241 gtgtcataga atggtaccta aacacaaata gcccgatttg atacactcaa atcaggttat
     6301 ttgtggctta tattatttac agatttcttg tatctgctga ttccatggca aataagcgcg
     6361 tagcgcttca ggtgtcatcg aagacagatt gggaatctct gtaaaaagat gatttaaata
     6421 tttgcggatg tttaacccat tcgcaacagc tgtctggata atactcaata taattccatt
     6481 tgatctagcg ccttttaagc ttttagaaaa tagccagttt tttcgaccga tcactaactc
     6541 tttgatggca cgctctgctc ggttatttga aagttccaag cgtccatcaa gcaacacaat
     6601 ctgaagtccg tctttatgat tgactgcgta ttgaaccgcc tttccaagct gcgattttgg
     6661 caaataagca aaactctcac accaatgaaa aaattcagtc atcacaggct gcaacgtttc
     6721 cttcctcgct acaaaccttt cttctgcgct aagcttatta aaggtccgtt cgattcgaaa
     6781 catcttgtca cagaagtcta atcctatttt ggcatgactt tttggggacg gttgtttacc
     6841 caatgcttca aaaaactttc tgcgaacatg cgcccaacaa taaacattgt ttaccggcag
     6901 ctttccatag gccgcgtaac catcagtatg caagaaaccg ctaaatgatt ctaaaaaact
     6961 ttttggctca tcaacggaac gcgaatttgc gtgctcgtag agcacaactt gtttttcttc
     7021 gcttttacct gaagcatata accagtaaaa ggtcttttct ttatgagtat tcaatacccg
     7081 atagcttgtc tcatccgcgt gtaagacctc ctgattgctt aattcttgat gaaggcaatt
     7141 gtagattggc tctaaaacca tttcacttac cttgatatgc caattagtga tttttttacg
     7201 atcaagaggg aaaccaagtg catgccattc tttttcttgt cgatatgccg gcaccttttt
     7261 aacaaatttt tcccaaatac tctgtgtcac aattgaagag gaacctagac tattattgat
     7321 caacggttta ggaacaggcg cttttttgat ttttgtttct cctaatgctt cacattctct
     7381 gcaatgatag gcatgttggt agatgacttg tttttctata tgggcaggaa catacaatgg
     7441 ctcttctcta agccttgtcg caccgatatc atgtaaagta tgaccacagt cttcacacaa
     7501 acaatcggca ccttttaatt cataatgaat aggaacttca ggcagacctt ctaatgaaag
     7561 tgctttgagt cccttgcgtt ttctgcgttt aaaagaagat accgtaatgg gttcctggct
     7621 ttcttgtcca gtgtgctctg gttcattaaa agttgagtcg tctaataaat cgatttgccc
     7681 atctacgggt ggtgtttctt tttttcgtcc aaacaagcgt cgattcattt cagcaatttg
     7741 ttctctaaga agagtatttt cctgttgctg gatttcaagc aactgtgtta attgcttgat
     7801 ttgtacaaga agttcagatt ccatttttgt catttttaca ccaccattct agcgattttc
     7861 ttacatctat tataccattt tatgacaacg aaaaacagac aaaatccttg atttaacaag
     7921 gttttcgtct aatatatcgc accttttttc gtttctttta gcgtacattt ttcttcgatt
     7981 gaaaggccag tcagtaagac ttcttttctg gaacgcggcc attgaaggta cccactttca
     8041 aatcttttgt acattaatac aaatccattt tgatcctagt aaagagcctt gaagcgatct
     8101 ttcttcgtac cgcaaaataa gaaaagcgct tgatcgtaaa catcaaggtc atattcttct
     8161 ataacgaaag ctgctagtcc atcaattcct tttcgaagat ctgtttttcc acaaacgata
     8221 aaaatatgtt tcaatgcagt tgcatcaatg agcattggca ttcaactctt ttaacaaggt
     8281 atacaaaaga tatttatcca ttccattata aataaatatt tccttatctt ccgatttgat
     8341 tctgcaagct agttttggct tggtgtcaat ttgcgatttt tgagccgcac ttgaaaattg
     8401 aactggtaca atatcttttt tattcattcg tttttctcct tctatatttg atagtttaat
     8461 tatagaagaa agataatcgg cgtacgagat actatcctat gacacgctta catttaattt
     8521 aatattttgc ctcaaattag ccacactttt tatagaacta agaggaaaaa tgaagttttc
     8581 ttcctattaa tatagttgtt tgaaatctta aatatagcgt tttacgatgt aatgggaagt
     8641 atggtgaata aatcaactat gttcgtacga agcatttact caactaattt aatcgaatct
     8701 ttcaataaac aaatcaagaa atacagccac cgcaaggaac agttccaaaa tgaagagtcg
     8761 atggaacgtt tcttagtctc gtcttttgat acttacaacc aaaaattcct aggtcgcagt
     8821 cataaaggtt tccaacaggc cgaaggcgag ctcgaacaaa tgctgagtca accgatggag
     8881 aattagaaac aatctacagt agggaagaac cattgacaca aaattattga cgctcccaac
     8941 taaaagtgtc aaaataatga tgaaatcatt tagttcttat aaattagaaa agagaaaata
     9001 aatattccat atctacttta ttggatccgt aacatgacta aattctgaat actttttaaa
     9061 gcttatcatt tggtaaaact gaatataagt taattatata gactgaaaat tttgaatttt
     9121 cagaatagaa taacttaaaa gtactgacaa aattttttaa aggggcaata ttaatgttct
     9181 gagataatca aatcctaccg aaaatatcaa aaatccaatg tgacacaatc agattgtgtc
     9241 tcattcgagg gaatcacaca atcccacgtg gctctaggca gaattgaatt tttgcttggg
     9301 gctttttcct tatattgatt ttgaatgaga cttattatat gttataatgc aattaagtat
     9361 cattggagag ggcgaagaaa atgaagacgg tagaacctat tcgagacaaa aaaaagatcg
     9421 atgcgatgaa ggcaatcttg gcttcgggaa aatatggcca acgaaatttg gttctttttt
     9481 cgattgggat caacaccgcc tatcgcattt ctgatttgcg tcaattgaag ctggccgacg
     9541 tgctggaaat ttcacgcggt cgcgtggtgg tcaaagatcg attagcgatg aaggaacaaa
     9601 aaacagctaa acataattcg gtctatattt ccaacaaatt gcgaaaaacg attttggagt
     9661 acgttcaaca ggaatttcct gatcaactcc ttcagcaaga ttttgacggt tatttgttcc
     9721 ctagtcgaaa agggacgaat gaaccgctca accgccaaag tctgtggcga atcattcatg
     9781 atgctggcac ggcaatcgga ttgaaagaca ttggcccaca ttccatgcga aaaacgttcg
     9841 gttactttct ctataaacag gggaccaaaa cagaaatcat tcagtccttg ttgaaccatt
     9901 cttctcagcg cgaaacattg cggtatattg gtattacaca agaagacaaa gatacagctg
     9961 taaaaagctt agatttataa tataaaagtt aagtttaaac atggatagtt ttaaattagt
    10021 agattgagtg ttgggaggaa agaatgagta cattatatat tataggaaac ggttttgatt
    10081 taagtcatgg tttaaaaact agatacactg atttttcaaa atgggttgaa aaaagtcata
    10141 gtgaaatatt cgataaaata gacacaatga taaatgaaaa taatgaggtg agaagtacag
    10201 ctgatgataa gataggttgg tttaattttg aaagtagttt gcatgagtta attcaaagtc
    10261 cagtctttga agattatgta gaagagtcta aggattatct cataggttat ggacatgaag
    10321 attggtcaga ttctgcccat catgattttc aaaatatggt ttatgattat gttgtttctc
    10381 tagaagaatt acaacaactt ttcaaggaat ggatacgcaa agaaatacag ccgacaatag
    10441 aacataaaat tccaaaatac caattttcgt ccgatgtact attctttaca ttcaattata
    10501 cagatgtttt ggaaattcta tacaagcaac cgaaagaaaa catatatcat attcatggca
    10561 gttatgacga tataataatc ggccactccg agaattttga caactttata ggagatc
//
LOCUS       DACCWV010000094        10546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCWV010000094 DACCWV010000000
VERSION     DACCWV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.095"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..786)
                     /locus_tag="IVW84_002048"
     CDS             complement(<1..786)
                     /locus_tag="IVW84_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7918231.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIE"
     gene            932..1735
                     /locus_tag="IVW84_002049"
     CDS             932..1735
                     /locus_tag="IVW84_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7918232.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1913..2860
                     /locus_tag="IVW84_002050"
     CDS             1913..2860
                     /locus_tag="IVW84_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7918233.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2928..3221)
                     /locus_tag="IVW84_002051"
     CDS             complement(2928..3221)
                     /locus_tag="IVW84_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7918234.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3249..3914)
                     /locus_tag="IVW84_002052"
     CDS             complement(3249..3914)
                     /locus_tag="IVW84_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7918235.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4054..4686)
                     /locus_tag="IVW84_002053"
     CDS             complement(4054..4686)
                     /locus_tag="IVW84_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918236.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4693..5760)
                     /locus_tag="IVW84_002054"
     CDS             complement(4693..5760)
                     /locus_tag="IVW84_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7918237.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5757..6488)
                     /locus_tag="IVW84_002055"
     CDS             complement(5757..6488)
                     /locus_tag="IVW84_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7918238.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6657..7136)
                     /gene="greA"
                     /locus_tag="IVW84_002056"
     CDS             complement(6657..7136)
                     /gene="greA"
                     /locus_tag="IVW84_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7918239.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7272..8447)
                     /gene="mltG"
                     /locus_tag="IVW84_002057"
     CDS             complement(7272..8447)
                     /gene="mltG"
                     /locus_tag="IVW84_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7918240.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8651..9820
                     /locus_tag="IVW84_002058"
     CDS             8651..9820
                     /locus_tag="IVW84_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7918241.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(10087..>10546)
                     /locus_tag="IVW84_002059"
     CDS             complement(10087..>10546)
                     /locus_tag="IVW84_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918242.1"
                     /translation="VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQV
                     TEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPF
                     VTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         3246 a         2100 c         1630 g         3570 t
ORIGIN      
        1 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
       61 ttcgctatac tgccaaatat ggacatgatg aacatccttt accccatcga ttgcttcaat
      121 ttcttgttcc attttttcat agtcgatagc aggtgcactt tgtatcaaaa tagcggcagc
      181 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
      241 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
      301 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
      361 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
      421 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
      481 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
      541 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
      601 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
      661 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
      721 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
      781 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
      841 caatcattca gctgtttcct taaagcactt gtatttcact tgatacttcg ctagaattgc
      901 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
      961 ctttatacga ttcaaagacc taaaggaggc gagaaatgtc ccattttgga aagccattgt
     1021 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
     1081 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
     1141 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
     1201 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
     1261 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
     1321 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
     1381 tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat ctataccact
     1441 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
     1501 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
     1561 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
     1621 aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg cttttgcagg
     1681 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
     1741 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
     1801 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
     1861 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
     1921 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
     1981 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
     2041 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
     2101 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
     2161 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
     2221 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga gtgacatgaa
     2281 atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac taatcattgg
     2341 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
     2401 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
     2461 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
     2521 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
     2581 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
     2641 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
     2701 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
     2761 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
     2821 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
     2881 aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta ttgtttttta
     2941 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
     3001 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catcaatttc cactttcttt
     3061 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
     3121 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
     3181 tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac ctactttctc
     3241 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaata
     3301 accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc gataaccgtt
     3361 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
     3421 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
     3481 tctgacaact ctaggtaatc taaaatattc tttgacacta aattatgcgc aatccttatt
     3541 cccatgtctc gctcgggatg gacgacgcga tcagctccga tcttttcaag tactctggca
     3601 tgatactcat tttgggcttt tgctaaaata tttggtacgc ccatttcctt taccatcaga
     3661 gtaactaaga tactggcttg aatatcttct ccaatagcga caattacatg atcgaaattt
     3721 cttatgccta atgatcgaag tgtcatttca tcctgtgcat ttgccacaac agcatgagtg
     3781 gctatattca tatattcatt gacccgatct tcattactat caatagccag tacttcttga
     3841 ccagcttcga tcagtgttcg gcagatactt ccgccaaatc gtccaagacc tacaattgca
     3901 aaattttgtt tcaccgtttt cctcctttag tatcttcttt tctcagtata acaaagcctt
     3961 tacaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
     4021 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttgaatgc
     4081 atagatagcg gcttgtgtac ggtcttccac ttctagctta gccaatatat tggaaacatg
     4141 tgtttttact gttttcagtg taataaacaa ttcgtccgct atttcctgat tactttttcc
     4201 ttcagaaatc agcattaaaa tttcattttc ccgattcgtt agttcttcat gtaatacagg
     4261 ctcatttcgc cgactcattt tttccatcat ttttgttgtc acttctggtt ctaatactcg
     4321 ctcgccacgt tgcgttgccc ttatagcatc agcaatttca tgtgcggtag aggtcttcaa
     4381 caaataccct gctgcacctg cttcaatggc aggataaact ttttcgtcat caataaaact
     4441 ggttacgata atgattttag cttctggcca atcttttaag attgcttttg ttgattcgat
     4501 accatccatt tcttccatga ctaagtccat taaaatgacg tccggtcgaa gtgtcatcgc
     4561 cttctcatag ccttgtcgtc cattttcagc ttctccaatt acttcaatgt cttcttgaat
     4621 agataaataa aaagagacgc ctaagcgcac catttcatgg tcatctacta ataaaacttt
     4681 tatcatcctt atctatgcct ccttcatcaa agggaccttg atttctacac ttgtaccttg
     4741 acctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
     4801 gttcaatcca taacttcccg ttttcgtttc attcatatta aaccctgttc cgtcgtcaat
     4861 aatacgtaac aaaagattat tgtctatttt gtgcaaatat acctctaatt cattggcttt
     4921 agcatgtctt aatgtatttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
     4981 ggaactagat agttttacat cttctacatc ccatttcagc gaaatctgga ttttattttg
     5041 caattccttc aataactgct cgataccttg ctttaaactt ttctcttcta aattgactgg
     5101 acgtagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgctac
     5161 cattttcaac tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt gctccgtcaa
     5221 tgccgacatc atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat gcagctcacg
     5281 ggctaatcga tgtctttcta attctaaaat ttcttctttg gtttgtccat ctacatattg
     5341 gggacggctc gttacaattt gtaattcact agatacttca atcatttttt cttttatctt
     5401 agtgatttcc ttatcaatat ctttgatgta taaatcgtcc gatgcacttg ggatcggaag
     5461 aagcaacaat ttggattcat aattgccaga agaaagtgca cgcagctttt cttcgatttt
     5521 cccgtatttt gtcttttgaa taagtgataa taatagaaag acaattaatc ctacacctaa
     5581 ggaaatggct aacaaatgga agataagcgg aacatataat aaacgtgcac gcagtaattc
     5641 tccccaccaa tgactttgat tgctggcata aaaataagtg aaaagtgaaa atagaataat
     5701 aaggaaagcc gcaatccccg aataaacagc tagcatcgct ctggatattt ttcccatcat
     5761 attcgtatca cctcgacatc tccaatcaag gtattagtaa taatcttcaa tctacgaggg
     5821 ttttcatcat aatcactgct gtagattttg atttgttcat ttttcaaaga aaattgctct
     5881 tcttcaaaca agacatttcc tactaatgtc gcatgctcta atcggatggc tacgcccaaa
     5941 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
     6001 ggcaaaagcg tattgcccaa atctatgata gtatcaccag aaatgacggc gatgttgata
     6061 tcgtcccatt catagacgtc gtttccaata cgttggttac cgaataattg ctgtcttttc
     6121 cgctcattat tatgtgtttt taattgctct gtttggatca tcatgatctg tttctttcgc
     6181 caaaaagcat ttttggttaa atctatccct gaaatctcta cacctttcag accaataaat
     6241 aaaacggcaa atacagccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
     6301 accaaacttc ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg tttgcgcata
     6361 gctagatata tattgaaaat accgaatatc actaaaagca aaagtccagt attgtttacg
     6421 atttgccaaa cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa acgccaagaa
     6481 ctattcatcg ctctttctcc ctcctgacac attctcattt catcatacct atttttttaa
     6541 ctgttggcta taggacttta gatgtaagat cgtttgttga aatctaaaat acacaaagag
     6601 aaaaagatct agagtgtatc atcagtaaga tgaactctct agatctttca gtcagactac
     6661 gcttgttcta ctttgacgat tttaactaac atatcgccac ctggagtagc aatagttact
     6721 tcatcatttt tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt ggaaattttg
     6781 ccagaaaacg gatctgcttc agcgcttccg acaatcgtgt actcttcttc ttctccatct
     6841 ggtagttcga tgaatgtaac tgttttacca attgatacct catctgcatc tacaccgtcg
     6901 ttatcgatga tttgagcaaa acgaatcata ttttcaattg ttgtgattct tccttcaaca
     6961 aatgcttgtt catctttcgc tgattcatat tcagagtttt cggaaagatc tccgaaaccg
     7021 cgggcaattt tgattcgttc aatgatttct ccgcgtttga ctgtttttag ttcttctaat
     7081 tctttttcta atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt
     7141 aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa
     7201 aaaagattac cacgcttttc ttcaaatgta aatgtttttc taaaaatgat caatgtctag
     7261 agcctgtttt tctaagaatt atttacatat ttttccacta attccatatg ttcttcataa
     7321 gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt
     7381 tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggtccaggt
     7441 ccatagccgg tattggtata caaattatat ggtgaatcta cagctgtatc ttcatacgtc
     7501 actaattctt tgtgttcacc taatgcgtat aaaacagaga tatctgactg aagtggcata
     7561 tttgctttga ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt ttcttttacg
     7621 ccttcttttt caactagaga agctagagtt aaaacttctt gtacagtcaa atttttctgc
     7681 ttgatagctg aataatagtt ttgcatgacc gtattcgtct tgttcaccat ttcaatcact
     7741 agatccttaa gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa gtaaccttcc
     7801 aaacggtatt ttgtgttctc agctttagat gcactttcca acagttctgg gaatttttgg
     7861 tgcaactcgt taaagaaagt ctcatcattc actaaatcta aaaattcttt cttgtcttta
     7921 ccagttacct tcgctacccg ttcagcaatc tgatcgatat cgtacccttc tggaatggca
     7981 attttcccat ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa ctgtttgcca
     8041 atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa
     8101 tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc
     8161 aaaatctcac caatctgctt gttagaagag ccactcggga tctcgacttg gactaattgg
     8221 tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca
     8281 agaaatccgc caataatcaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc
     8341 ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta
     8401 ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct
     8461 tactatcaag ttcgtaactt tattcattat acatgatatt tgaattggcg tgaatgattt
     8521 tatttaaata ttctatcaaa aatgtaagta gtaaatccat tactataaga ttaaaataaa
     8581 tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa
     8641 ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat
     8701 cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt
     8761 ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat
     8821 actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga
     8881 ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg
     8941 ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc
     9001 ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc
     9061 tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat
     9121 ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat
     9181 gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac
     9241 ctctcattat gattcattga actttaccat attgactgga attttgatca aattaacaaa
     9301 tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac
     9361 aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa
     9421 agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg
     9481 gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc
     9541 tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca
     9601 tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga
     9661 ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag
     9721 caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta
     9781 tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa
     9841 tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt
     9901 gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag
     9961 aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat
    10021 tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa aatatgtgct
    10081 agcaaatcat tcatggcttg ttcttatttt taattttatg caataaaaaa gaagaacaat
    10141 tagcgtgatt atagaaataa ataatgagat tttagaaaaa aatgtgtatg aatacctgat
    10201 tgttacttta tttattccac tttcactttt tactgtaaca aaaggacctc cttgttttag
    10261 tactgtagca ttttttgaat taacagtaac cgttaatcct ttataccgga atacaggtaa
    10321 aactattgtt ttgccttttg aagatttaaa cgtaaatgta atttcatctt tatttggaga
    10381 aaagctacta taaataactt gaccatcttc tgtaacttga tggtttttca aaagcaattg
    10441 atctttttta tagcctatag gtacataatc aaaattctca aattcttcaa gtttattttc
    10501 catttgttca ttagaataag ttccttgaaa attcatttct ttcacc
//
LOCUS       DACCWV010000095        10519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCWV010000095 DACCWV010000000
VERSION     DACCWV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.096"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            35..658
                     /locus_tag="IVW84_002060"
     CDS             35..658
                     /locus_tag="IVW84_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918243.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1108..2157
                     /locus_tag="IVW84_002061"
     CDS             1108..2157
                     /locus_tag="IVW84_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7918244.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2154..3035
                     /locus_tag="IVW84_002062"
     CDS             2154..3035
                     /locus_tag="IVW84_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7918245.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3038..3808
                     /locus_tag="IVW84_002063"
     CDS             3038..3808
                     /locus_tag="IVW84_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7918246.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4087..4941
                     /gene="aroE"
                     /locus_tag="IVW84_002064"
     CDS             4087..4941
                     /gene="aroE"
                     /locus_tag="IVW84_002064"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7918247.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5119..6282
                     /locus_tag="IVW84_002065"
     CDS             5119..6282
                     /locus_tag="IVW84_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7918248.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6399..6809
                     /locus_tag="IVW84_002066"
     CDS             6399..6809
                     /locus_tag="IVW84_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7918249.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6983..9211
                     /gene="topB"
                     /locus_tag="IVW84_002067"
     CDS             6983..9211
                     /gene="topB"
                     /locus_tag="IVW84_002067"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7918250.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9349..9666
                     /locus_tag="IVW84_002068"
     CDS             9349..9666
                     /locus_tag="IVW84_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918251.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9826..10062
                     /locus_tag="IVW84_002069"
     CDS             9826..10062
                     /locus_tag="IVW84_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7918252.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10059..10457
                     /locus_tag="IVW84_002070"
     CDS             10059..10457
                     /locus_tag="IVW84_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7918253.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3668 a         1766 c         2229 g         2856 t
ORIGIN      
        1 ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg aagtagttaa
       61 atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa atccaagtaa
      121 gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag ttgcaatgtt
      181 tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt cgtccatacc
      241 aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca aacaaagccg
      301 ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca tgcttgatgt
      361 ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt atccgatcgt
      421 ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag tgacatttat
      481 tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag gaataactga
      541 ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata tcaaacaatt
      601 agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg ctaattgaga
      661 cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca gaacaagtga
      721 tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga aattcaaaaa
      781 ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac ttatcaaaac
      841 gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt gtattaatgg
      901 tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt atctatgcta
      961 gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg taaagacaat
     1021 gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta taataaataa
     1081 aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac agttgtagta
     1141 attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg cgaaaaagaa
     1201 agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca atacgtgagc
     1261 catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga agaaggatta
     1321 aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca gagcaagcta
     1381 gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg aatcgcaaca
     1441 cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt aggtgctgta
     1501 acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg taatgtaaca
     1561 ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga tttgttgcca
     1621 aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa ataccaagta
     1681 gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata tgcggtgcct
     1741 tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga ttttatctat
     1801 gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga agcaaacaaa
     1861 gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg actagcaaca
     1921 atcgggatca atcaatatca attagggctt caaagcggta agatggcggc agaacttgcg
     1981 agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg ggatacagtg
     2041 atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat gaaggaaaaa
     2101 gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga caaatgatcg
     2161 tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt ttcatgactt
     2221 atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta ggtgctgctg
     2281 tctgtgtgac agctattact catggggtag cgccgattgt agctacatta ttaggcgtag
     2341 gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga aagataccgg
     2401 ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg tttgtgatgc
     2461 gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc catcgctttg
     2521 ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc atcttgattg
     2581 ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg acaggagaca
     2641 acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt ttaggtttaa
     2701 ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac gacggatatg
     2761 cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc atcatcggtg
     2821 aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt gtcggcagta
     2881 tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg acttatctga
     2941 agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg aagaaagcac
     3001 tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat tgacaatcaa
     3061 aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat tggatgaaat
     3121 taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg gtgctggaaa
     3181 aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat cgatctcttt
     3241 taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc tttctcgagt
     3301 tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa atttgctact
     3361 tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa atcaaaaaga
     3421 agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact tggatactcc
     3481 agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg cgacgatcac
     3541 agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta aaacagctaa
     3601 agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt gtctcatgat
     3661 cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac tccaaaaagg
     3721 gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg gcgacctttt
     3781 gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc agtagaagga
     3841 cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa taaacaatta
     3901 aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt aaatagaata
     3961 ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca cctagacgtt
     4021 tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga acaaaggagg
     4081 aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc ggcttctcac
     4141 agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga tgcggtgtat
     4201 ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat tcgaactttt
     4261 gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc ttatctggat
     4321 gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca cgaaaagggt
     4381 cgtttgattg gctataatac tgatggtgcg ggcttcatgc aatccgtgca agaagctggg
     4441 atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc aaaggcagtc
     4501 actgttcaag cagcgttaga cggcgctgag gaaatcgtag tttataaacg aaacaaccaa
     4561 acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc ttgtaagata
     4621 aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga aagctgtctt
     4681 ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc gctcttagat
     4741 ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc tagagaaaca
     4801 cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact tggcatgttg
     4861 atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc gatccatgtg
     4921 attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt ctcgcataaa
     4981 cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat tttttattaa
     5041 attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt tatcagtaag
     5101 aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca taagaaggcg
     5161 aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa acgaacgcgt
     5221 atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc atttgaacat
     5281 ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga acaacaaaaa
     5341 aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg aagaaagcga
     5401 tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc aggtatctca
     5461 ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc gacttcagaa
     5521 acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct tggaagtgat
     5581 acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt ggatggtcct
     5641 tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat cccaggggta
     5701 ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt gcgtcaaact
     5761 ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt caaatcgttt
     5821 gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga aaaagatctg
     5881 aatctggatg gtgtagatat taaaaaaggg ggccagaaga tggatggaca tgttcttttg
     5941 cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag acgccaacag
     6001 caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat ccgtgcacct
     6061 tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg gttcttgacg
     6121 aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag tgtgccagta
     6181 gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga aattgataat
     6241 caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa aagatcaaga
     6301 agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta aaaaacgctt
     6361 tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat ctgaacttca
     6421 aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact gaagcatcag
     6481 gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca gaagaagcaa
     6541 aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg tttgcagata
     6601 cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta gtcatccaga
     6661 caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt ggtaagattg
     6721 agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact gatcgatttg
     6781 gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc tggaaacatc
     6841 aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt cgactatacg
     6901 gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag tccgcaaaaa
     6961 attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac cttcacaagc
     7021 aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt atgaaatcag
     7081 cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt tggtagatat
     7141 ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa atttgcctat
     7201 tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat tttcgatcgt
     7261 aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg atcgagaagg
     7321 tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg aaaagaacta
     7381 tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt ttaagaatct
     7441 ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc agaacgctga
     7501 ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac gaaaagggat
     7561 tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga tttatcagct
     7621 gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag gtttaattac
     7681 gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta aaacccaaga
     7741 agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa acggacggat
     7801 tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt cgctatctag
     7861 tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac ttgatgctgc
     7921 tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct tttacattac
     7981 agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat ttttaggaat
     8041 agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg cgaaaaaagt
     8101 tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac gctttgcgaa
     8161 gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg tggctatgtt
     8221 tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc aattaaagct
     8281 aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac gcatcaagtc
     8341 aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag cagaagtaga
     8401 actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag gaacgttgat
     8461 cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag aaattctcaa
     8521 agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa atcttaagca
     8581 gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag gaatcactct
     8641 ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg cgcgctggga
     8701 agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt tagagaatat
     8761 caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca aggtagactt
     8821 ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa acgatcaatt
     8881 aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa tcgctacatg
     8941 cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga agctgactaa
     9001 aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca aaggttttac
     9061 tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga caatcggttt
     9121 ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca aagaaacatc
     9181 aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc ggaatattta
     9241 ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga atcaagcaaa
     9301 aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt gaaaaaagtg
     9361 cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta ccaagcattt
     9421 ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca aacagcatat
     9481 tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac ttattttttt
     9541 cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt ggtggtggta
     9601 gcggctatga tcattattta ttttctacca gttattacac aagtatttca tcaatatttt
     9661 ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc tttgtcattt
     9721 tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat acttggatat
     9781 aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa aaaaatgaaa
     9841 aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc agaagtaaaa
     9901 gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc tgatggttct
     9961 gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga ttcgatcatg
    10021 aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt tcgactaaag
    10081 aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa atgatcggtg
    10141 taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca gtattcaatc
    10201 aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat ttagctaaaa
    10261 aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg ttgtttcttc
    10321 aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt atcataatga
    10381 ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt tatttacgac
    10441 aaaccgcaat aaaatgaacg aattaaaaac tttatttctc aattagatca gcatgtcatt
    10501 aaggaatatg ctgattttt
//
LOCUS       DACCWV010000096        10422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCWV010000096 DACCWV010000000
VERSION     DACCWV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.097"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            122..862
                     /locus_tag="IVW84_002071"
     CDS             122..862
                     /locus_tag="IVW84_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918254.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1153..1821)
                     /locus_tag="IVW84_002072"
     CDS             complement(1153..1821)
                     /locus_tag="IVW84_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7918255.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            1955..2242
                     /locus_tag="IVW84_002073"
     CDS             1955..2242
                     /locus_tag="IVW84_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918256.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            2658..4196
                     /locus_tag="IVW84_002074"
     CDS             2658..4196
                     /locus_tag="IVW84_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7918257.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            4193..5158
                     /locus_tag="IVW84_002075"
     CDS             4193..5158
                     /locus_tag="IVW84_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918258.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            5145..6467
                     /locus_tag="IVW84_002076"
     CDS             5145..6467
                     /locus_tag="IVW84_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7918259.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      6530..6634
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6708..7451
                     /locus_tag="IVW84_002077"
     CDS             6708..7451
                     /locus_tag="IVW84_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7918260.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            7466..8458
                     /locus_tag="IVW84_002078"
     CDS             7466..8458
                     /locus_tag="IVW84_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7918261.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            8470..9207
                     /locus_tag="IVW84_002079"
     CDS             8470..9207
                     /locus_tag="IVW84_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918262.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            9197..9919
                     /locus_tag="IVW84_002080"
     CDS             9197..9919
                     /locus_tag="IVW84_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7918263.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(10003..>10422)
                     /locus_tag="IVW84_002081"
     CDS             complement(10003..>10422)
                     /locus_tag="IVW84_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7918264.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3461 a         1793 c         2172 g         2996 t
ORIGIN      
        1 actatatata tatttatgaa attcatcgct attttcttaa aataatcagc gcattgctac
       61 tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa ggatggtttg
      121 aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg agttaggcgt
      181 aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc ttttgactga
      241 acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca cgaacaccga
      301 gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc gtaaggaaaa
      361 tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat cagaaatcac
      421 atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac aaagtgaaac
      481 aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg
      541 ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta gagaaagcaa
      601 aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt
      661 cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg tacgaacatt
      721 caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag
      781 ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa actctttggg
      841 agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca gttggaaagg
      901 aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg tatcctcaaa
      961 tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg gcaaaataaa
     1021 aaaacaaact attttcgcag atcgtttgca aaaatagttt atttttttat aaagaagatt
     1081 ttactgaata atatcatttt agtttacata atgatatttc tgtaaattaa ataaagtagt
     1141 gaaaaataag aatcagtgct ctaactgttc aagaggatca agtggttcaa atgtttctgt
     1201 agtctgtttt gcttccaatt ggggcaaaac agatccaatt tccagatact tcactttatt
     1261 caatgtataa gatagaatag aacagctaag taatgtatac agaaaatgtg tgagatccaa
     1321 ataaagcaag gagacaacta aaataccaaa agtaatcaag aaaagacttt tgccaatctt
     1381 aatatgaaaa tatttttcaa tggcttgata aatgatatct gttccgcctg tgcttccgcc
     1441 gaatcgaaag acgagaccgc aaccaatgcc agaaagaata cctgtcatca aactattgat
     1501 cagtagacta tctacagcaa aatgtatatg aaggctctcc cataaatgta aaaagaaaac
     1561 tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa gaaacaatga
     1621 gataagcaat aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga atccaaaaag
     1681 attatgcaaa agcaaagcaa taccagtaac cccaccatct gccagcttag atggaatatt
     1741 gattcctgtc acagctaatc cgtagattcc tgcgcctaaa atgattccgc acaattctgt
     1801 aaataaattt aattttttca tttctagcct cttttctttt gaaaattttc agcaacttct
     1861 ttattatact gacttatact cttacttcaa gaggaaaatg aaaaaaaatt atttttggct
     1921 gtttcttttt atttatcaaa taaggagtgg aaaaatgcga gcagcagact tattgacaat
     1981 tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt tttttggctc aagaacaaaa
     2041 actggtgcct gtgacaatgg tccagataaa taaagcaaat caatttatct ttttatttga
     2101 acacggcaaa gcaccacttg ctatgaaaga catctttctt tttttgatga agaaccgtga
     2161 gaaagctgtt tattatcagt caaaagaaga actcattcct ctttatggag tgaaagagat
     2221 agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg atgtcaaaac
     2281 aaccattttt tacatttttg gcaaaaaagt gtcatgatac agtataatat tcgttgaaca
     2341 ggccaataaa ggtgttctcc cccacgagat ttctacttta ttggctgttt ctttttatta
     2401 aaataaaagt tgtgtgtgta aaaaagatat agctgattcc aagtgacaat ttcgtataga
     2461 tttttatggt attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac tttgttgctt
     2521 ggattctttt tttgtcagca tatttagagg tatttttttc ttttttttgt tcgtgctgta
     2581 aggttaaggc aagacaagtt agttgggaaa aaggaaagaa atgtttatac ttaaagaata
     2641 acagaaagga aggtcgcatg aataatttta tacaaacatt aagtgaaaga aaaggcgagt
     2701 tgctggaagc tgcatttcaa catctgtcca tttctctcat ttctttgctg attgcagcac
     2761 tgattgctat tcctttagcg atttgggcag ccaatcataa aaaaatggca gaagtcttat
     2821 tgcagatcac gggtgtgctc caaacgattc cttcacttgc cttattaggt ttacttatcc
     2881 catttgtagg gatcggaact gttccagcat tgattgcact tgttatttac gctttgcttc
     2941 cgattttcca aaacacgtat attggtctag ctgaaattga cccgtctatt gaagaagcag
     3001 cggtagcatt tggtatgtcc agaatgagac gcttgctgaa agtagagctt cccatcgcat
     3061 tacctgtcat tatctcaggt atccgaactg ccttagtttt aatcatcggt acggctactt
     3121 tggcagcatt gatcggagca ggtggattag ggacatttat tttgttaggt attgaccgaa
     3181 atacacctgt tttgacattg attggtgcga tcagttctgc cttgcttgca attatattca
     3241 gcggactgat tcgctggttg cagcataaga aacctcgata ttccatcatt actttagcag
     3301 cagtggtgtg tttatttggc ggcgtttctt tatatcagtc tgccttgttt aaaaatgaaa
     3361 ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa atgtataaag
     3421 aattgattga agacaacaca aaaacagaag ttgaattaaa accgaatttt ggcaagacga
     3481 gttttttgtt caatgcactg gatcaaggtc aaatcgatat ctatcctgag ttcacaggta
     3541 ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa aagtgctgat ttgacaaaag
     3601 aaaaaacata tgacgaagca gaagaattat tgaataagca attcgatatg acattattaa
     3661 aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa gagatctttt gcgaacgaac
     3721 atcaacttaa aaagatttcc gatttggcag ctatctctga tcagatcaaa gcgggattca
     3781 cactggaatt tattgatcgt tcagatggat ataaaggaat ccaagaagca tatggcttga
     3841 atttttcaag tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc aataatggag
     3901 atgtcaatat tgtggatgcc tattccacag atagccagct caaggagtac gatctagtga
     3961 ctctagaaga tgatcgaggg ttattcccag cttatcaagg cgcaccgctg atgaaaaaag
     4021 attttgctaa agaacatcct gaaatcgtta aagcactgaa taaattaagc ggaaaaataa
     4081 cagaggatca aatgattgat atgaattacc aagtcaatgt cgaaaagaaa aagccgacag
     4141 aagtagcacg aatcttcctg aaaaatgaag gattagtaaa ggaggaagat cagtgactaa
     4201 attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca tcgatgacct
     4261 aaatctggca atcaaaaaag gagaaatctt tgttttggtt ggtccttccg gtagtggaaa
     4321 aacaactact ttgaaaatga tcaatggatt atctaaacca tcagcaggag acatctactt
     4381 caaaggaaaa tcgttaaatg aatataatct gcagaaaatg cggtggaata tgggatatgt
     4441 gctgcagcaa atcgctttgt tcccgaccat gacagtcaag caaaatattg aagtcatccc
     4501 tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt gtagatgaac ttcttcaaaa
     4561 agttggctta tctcctgata tttatcgaga tcgtatgcct agagagttgt ctggaggaga
     4621 acagcaacgg atcgggatca tccgagctat tgctgcaagc ccagatgtga ttttgatgga
     4681 cgagccattt agcgcattag atccgatttc aagaaactca ttacaagaat tggttttgtc
     4741 cttacacgaa gaattgggca cgaccatcgt tttcgttacg cacaatatgg aagaagcaat
     4801 caagctaggt gatcgtattg catttatgaa ggatggagaa attatccaat gtgacacacc
     4861 agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg catttctttg atgaaccgaa
     4921 acagacgaaa gagtggcgag tagaagattt agtcgtcaat ggatatttct taaatgaaat
     4981 accagaaaat gctcgtcaac aagtttgctt tgatacgcct atgaaagaag tatattcgct
     5041 tctttcggtg tatccaagca ttgtgatagt agaaaagcaa agaagtattg ggtcgctgac
     5101 aagtaaagaa atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa acatatgatg
     5161 caatcatcat cggttcaggt gtgagcggct taagtgctgc ttatggattg aaagaagcag
     5221 gtaaaacagt actagtggtc gaagaagatc tatggggcgg gacttgtcct aaccgtggat
     5281 gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg taatcgggta aaacaattga
     5341 gtggaaaagg ttttaacgag atcccaactg ccaactggga agaacttcaa aaattcaaac
     5401 gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa gctgaaatcg
     5461 atcatctttc aggaaccgct cgttttttgg atgattcatc tattgaagtc aacgaagaag
     5521 tcttccatgc ggattactta gttttggcaa ccgggcaaag acctacgatc ttaccagtgg
     5581 agggaaagga atacttgaaa actagtgcag actttttatc attgcctgta ctgcctaaag
     5641 aaatcatttt tatcggtggt ggttatattg cttttgagct agctacgatt gccaatgctg
     5701 cagggagcaa agtaaccatt gtccatcata atcaacggcc attaaaagaa tttgaggcgt
     5761 ctttggtaga ggaagccgtc catcaaatgg aagcatctgg cattcagttt gcttttggtg
     5821 ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg tttagtcgga aaagagacgg
     5881 aattagtagc agatatgatt ttttgtgcga ctgggagaca gccgaataca gaatctttag
     5941 cacttgagca agcgaatatc gtctttgata aacacggaat tgcagtcaat gattatctgc
     6001 aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa accccaaaat
     6061 tgacaccagt cgccactttt gaaggaaatt atgtagctaa gcggataacg gatgcaacaa
     6121 gcgaacctat caaatatccg attataccta cgatcgtcta cgccagtcct aaacttgctg
     6181 aagtaggagt tacaaaaagt cacgcctctt cgtcagatca agtggtagag atggatctta
     6241 ccagttggtt cacctatcat cgtgtcaatg aaccagttgc taaggctgaa ttgacctttg
     6301 accagcaaaa ttatttgatt ggtgctgcag tgattagcga gcaagcagac gaattgatcg
     6361 atgatttgac actcgtcatc aatcaaaaat tgactaaaaa agagttggat agctatatca
     6421 tggggtatcc gacattggca agtgatctct cctatttatt gaaataatca gcatgataac
     6481 taaagcgctt gttgcttttt ccttcttgtg ctacacttta aaaaatgaat acctgtttct
     6541 ggggatgtcg tagacgacat gagagtaagt tgtgaactta gacccattga acctgtcagt
     6601 taatactggt gaagggagaa acatagcaga cgcttgaagc gggttactat gtcgtgccca
     6661 tttcaagcgt tttttgttat tcatattaga agaaaggagg taacaaaatg aagaaaaatc
     6721 tttatccagc agtcagtatg gcattcattt taattatttg gcagattgtt gtcatagttt
     6781 ggaaaatacc tgcctttatt ttacccggac ctgctgctgt attttctgca ttagcggcag
     6841 atgcacaagt attactttcc cattctgtca cgacattaaa tgaagcagtg cttggtttgg
     6901 taatagctag cgttctttct tttattactg ccttggcaat ggattattgg aagtttttag
     6961 aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt gccaatcatg gtgttagggc
     7021 cattacttac cctatggttt ggtttcggta tgcttccaaa agtgatcctt gttgtcttga
     7081 taagctattt tccgatcgta gtttcttttt cggatacatt gaggaaagta tcaaaagagc
     7141 aaatcatctt tttgaaaacg atgggggcag atacagggaa gatttatcga atatataaaa
     7201 taccagcggg tttatccggc ttcttttccg gtttaaaagt ggctgctact tattgtgtca
     7261 gcggagcagt cgttggagaa tggctaagcg ctcaagcagg cttagggtat tacatgatac
     7321 gcgtaaaaaa cagctaccag atcgataaag tttttgctgc gatcatttgt gtcattctat
     7381 tgagtttatt gctgaatggc gcttgttcat tattgaaaaa agggtattat cgtatgcttt
     7441 atatttttta agagaagggg atcaaatgaa aaaattatgg cttttattac cgctactgtt
     7501 gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa taaaggattt
     7561 gaaaaaagtg acacttgttt tggattatgt accgaatact aatcatactg gcatctacct
     7621 agcaaaagaa aaaggctatt ataaagaggc tggtttaaat gtccaaatca tcgaaccagg
     7681 agataattcg acaagtattg gtctagtagg tgcggacaaa gcacaatttg gtgtaagtta
     7741 tcaagaagat gtcacttatg ctcacgcaga tggacagaat ataccagtca aagcaattgc
     7801 tacagtgatc aaacataata cttctggatt cgccacccta tcagacagta acatccattc
     7861 accaaaagat tttgaaggaa aaacatatgc aggttggcaa agtccaagtg aagaagctgt
     7921 cttaaaagct gtgatggaaa aagacggcgg ggacttttca aaactgacaa tggtaggaag
     7981 caatggagaa gggccagaaa gcttaggaaa atcctcagat atccaatggt actttgaagg
     8041 ttgggatatg atcaaagcaa aagaagcggg tattgaacta aactatatcc cattgaaaga
     8101 attagatgaa cggttagatt actatactcc ggtaattatt acgaatgatc aactgatcaa
     8161 aagtgatcca gaattggtac agtcttttat ggatgccacg aaaaaaggct atcaggaagc
     8221 tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag aaaatgatcg
     8281 gacgttttta gaagaatcac aagcattctt gtcaaaaaat tatacggatg atcctgagaa
     8341 ttggggattg atggaagaaa aagtttggcg caattataca gcttttatgc aggaaaacgg
     8401 attgatcaag caagatgtac cttcgcaaga actattcact aatcagttta taaaatagga
     8461 gtgatagata tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa cctgaaaata
     8521 ttagaagaca tccatttcac tatccctaaa gataccttta catcgattgt cgcgccaagc
     8581 ggagccggta agtcaacatt gctgaaaact ttaacaggag tccagcctat gacttctgga
     8641 acgatcaaag tagacgatca aaaagtaact ggtctccata cagcatttag ttatatgccg
     8701 caagaagata tgttacttcc ttggctgagc gtctatcaaa acgttacgtt atatcaaaaa
     8761 atcaatcatc taacaatcaa tgaagaacaa gtaagggatt atctggatat ctttgggtta
     8821 aacggctatg aacatttttt gccggaacaa ttatctggcg ggatgaagca gcgaacggcc
     8881 ttgttacgaa caatcatgaa tccttcttct tacttgcttc ttgatgaacc atttggagcg
     8941 ttagatgcta tgaccagagg gcagatgcaa gactggatgt tgaagctgcc taaaaaagca
     9001 aagcggacta ctttacttgt gacgcatgat atcgaagaag caatttattt atcagacagg
     9061 attcttgtgc tttccgcccg tccggcacat gtgattgctg agattcaagt gcctgaaaaa
     9121 cgacggtcac gtgaatggct actgcagcaa tcagagttga agcaaacgat ttatcagtta
     9181 ttggcaggtg aattagatgt taagtgatgc tcattgtcat ttagatggga gtcagagttt
     9241 agcactcctt cagcaggaac acacgatttt gacgatcatc aattgtgatt cgccagaaga
     9301 atggaaggaa aacagacagc ttgcagcaag taagactcaa gccttaagtt acggtatcca
     9361 tccttggaaa gcagatagct atacgtttga acaagttgag ccttttttga aaaaagcaag
     9421 gatcatcggt gaaatagggt tagataatat ttggacgaat gtaccgatga ctacacaaaa
     9481 aaaagtattt gagcgacagc ttgcttttgc agccataaat gagaaacctg ttgtactgca
     9541 cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa gcttacccta atcactattt
     9601 gatccattgg tattcttctt tggactatca acaagactat attgatttag gctgttattt
     9661 cagcattggt cttgatttga aaaagaatcc agcagtctgg caactagcta aagcagtccc
     9721 aatcgaccac ttacttattg aaacggatgg actacatgca gcagagtggg ctttaggggg
     9781 gattttttca gaaaaagatt atgtctcact gcttgaagaa cagttaaaga caatcgctga
     9841 gatcaaagag ctatcagttg aagaagttaa aaaaaatact tatcaaaact tggaacgttt
     9901 cattagatta ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc tctttttggc
     9961 tttctttgta taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc aaccgtttgc
    10021 ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc
    10081 tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta
    10141 acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat
    10201 ccattctttt gcagagcttg tgcttgctgg agtagcagca gtttctgtta cagcaggagc
    10261 aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt cttgtacaac
    10321 aggttgttca gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg
    10381 tgcagagcct tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DACCWV010000097        10318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCWV010000097 DACCWV010000000
VERSION     DACCWV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.098"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(71..904)
                     /locus_tag="IVW84_002082"
     CDS             complement(71..904)
                     /locus_tag="IVW84_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7918265.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="IVW84_002083"
     CDS             complement(1208..3373)
                     /locus_tag="IVW84_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918266.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="IVW84_002084"
     CDS             3633..5927
                     /locus_tag="IVW84_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7918267.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="IVW84_002085"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="IVW84_002085"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7918268.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="IVW84_002086"
     CDS             6578..7585
                     /locus_tag="IVW84_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7918269.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="IVW84_002087"
     CDS             7607..8626
                     /locus_tag="IVW84_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7918270.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="IVW84_002088"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="IVW84_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7918271.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DACCWV010000098        10231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCWV010000098 DACCWV010000000
VERSION     DACCWV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.099"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            44..2740
                     /locus_tag="IVW84_002089"
     CDS             44..2740
                     /locus_tag="IVW84_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918272.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2817..3305
                     /locus_tag="IVW84_002090"
     CDS             2817..3305
                     /locus_tag="IVW84_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7918273.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3317..3811
                     /locus_tag="IVW84_002091"
     CDS             3317..3811
                     /locus_tag="IVW84_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918274.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3832..4641
                     /locus_tag="IVW84_002092"
     CDS             3832..4641
                     /locus_tag="IVW84_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918275.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4638..5459
                     /locus_tag="IVW84_002093"
     CDS             4638..5459
                     /locus_tag="IVW84_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7918276.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5510..7609
                     /locus_tag="IVW84_002094"
     CDS             5510..7609
                     /locus_tag="IVW84_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7918277.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7606..8724
                     /locus_tag="IVW84_002095"
     CDS             7606..8724
                     /locus_tag="IVW84_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7918278.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8763..9641
                     /locus_tag="IVW84_002096"
     CDS             8763..9641
                     /locus_tag="IVW84_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7918279.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9832..9984
                     /locus_tag="IVW84_002097"
     CDS             9832..9984
                     /locus_tag="IVW84_002097"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918280.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3140 a         1679 c         2286 g         3126 t
ORIGIN      
        1 tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag
       61 aatagacata ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa
      121 aatgaaaaag agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat
      181 gtcttctgtc actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag
      241 ccattatttg acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt
      301 tttgtggaag cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc
      361 ctttcattct gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc
      421 cgctgtcaaa tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg
      481 aaaaagtttt ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa
      541 agatgcacca tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc
      601 ggcaacactg tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg
      661 tttattggca acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa
      721 agaaaatcaa gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga
      781 aaatgtaaat tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga
      841 aaattattta ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt
      901 tgaagatcgc cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc
      961 ggatgtttta caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca
     1021 tccctttagt gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt
     1081 gtatagtcga acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt
     1141 aaatatgctg gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa
     1201 tcaaaagata aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat
     1261 tactcagctg ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt
     1321 acagcatttg gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat
     1381 gattcagatg tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac
     1441 gagtggacct gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac
     1501 agacttaacc gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg
     1561 taatcagttc ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact
     1621 gcttattgtc gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt
     1681 gcttgctcac ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg
     1741 caattatgtt tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga
     1801 atcgacaagg aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga
     1861 tatgggctct ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact
     1921 tcttctgaat aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa
     1981 tgcgccattt aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata
     2041 ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat
     2101 ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt
     2161 cacgaaggac tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga
     2221 caagacaaaa ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat
     2281 taatgtttat gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc
     2341 tgaaatgaag ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt
     2401 ggaaggaatt gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga
     2461 aacagtcatt tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact
     2521 aaatctctat atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa
     2581 atctgaagaa ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag
     2641 aagtattttt caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc
     2701 gctgctctat gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta
     2761 atctgttttt tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt
     2821 ttttaaaagt tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc
     2881 atatgaagaa aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta
     2941 ttcaattact gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt
     3001 gtgattttga acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt
     3061 ttgtttttac cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg
     3121 agaaagagat tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag
     3181 tattggcaac caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac
     3241 aggaattaaa gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt
     3301 tttaggagga agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca
     3361 agtagcgatg acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga
     3421 ggcggctacc aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa
     3481 agtcattatt aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga
     3541 catgcgcttg atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt
     3601 cccaacggaa atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga
     3661 agataagaaa accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa
     3721 agaattagtt gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg
     3781 ttcagctatc tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat
     3841 gcatttgtag tcgctttggc cgtttttatt ggagttgctg gacatgaaat gttcgggatg
     3901 tccatgctga gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg
     3961 cagacggggt tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc
     4021 ggaatctcta gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc
     4081 aaaatgggag gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga
     4141 ttacagttaa tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc
     4201 aaatttgcaa aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt
     4261 gtccactatg cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt
     4321 gtcaaaagcg tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga
     4381 aatctgctgc cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt
     4441 gctgtgtggt ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca
     4501 attgccgcag ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa
     4561 aatatatctg ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa
     4621 gaggaggatt tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg
     4681 ttcggaagat gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg
     4741 gcggcggtgg tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag
     4801 aagatcgagc tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg
     4861 cgtcctttgt catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt
     4921 ttgacgtgga aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg
     4981 gcgattcatt gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag
     5041 ccaatgccgg aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt
     5101 acttgatgcg atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa
     5161 agctgtatgc gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga
     5221 tcatggtagg tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa
     5281 tgaagggcga agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg
     5341 taccgttatt ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca
     5401 aaattatttt tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt
     5461 aggaagctgc agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa
     5521 tgaagtaatt tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt
     5581 gactacttca aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt
     5641 cgatgtaagt aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt
     5701 gaccttttat cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca
     5761 gctctctcaa accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt
     5821 gtcagaatcg gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat
     5881 tcatcattat tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa
     5941 gcgtttaagt atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca
     6001 agtttcagtg aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt
     6061 gatttctcaa aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat
     6121 tgcttttgtg gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga
     6181 aaaatggctg aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc
     6241 aaacgataga gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg
     6301 tatgaatcga cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg
     6361 aatttggaat aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt
     6421 atgtcaaaaa acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt
     6481 tacaggaggt atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct
     6541 ttattttgaa catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa
     6601 tgcttcaaga attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt
     6661 tccatctgtt tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct
     6721 ttgtctttgg aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag
     6781 tcttagcaag cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat
     6841 gactatcgat ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac
     6901 aacctattca gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag
     6961 tgatgtgatt gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac
     7021 tacgttgatg cctttttatc aatttttaga taataaagat catttatatc agaacaccat
     7081 ggccttttct gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg
     7141 gggattagaa tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca
     7201 caaccctcaa gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg
     7261 ttcgtggtgg tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt
     7321 tggctgcggc aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata
     7381 tttcggattg aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt
     7441 ttcttggaag aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga
     7501 gttgagtatt tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct
     7561 tcacgtagaa aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa
     7621 aatattaccc aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc
     7681 gagaaaggta aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc
     7741 gcaattccgc tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata
     7801 attcgtcata tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt
     7861 gatttaatct taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta
     7921 ggggatttct tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg
     7981 gttaatccta cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa
     8041 attacgatcg agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac
     8101 gctttggtgg acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa
     8161 cgcattacca aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat
     8221 tacgtgggta cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag
     8281 tttaaattct acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa
     8341 gattattttg gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg
     8401 aaaaactatt caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga
     8461 gatcgttttg aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca
     8521 cgacatggtc tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg
     8581 gtcactttac aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt
     8641 gctagtgtcg cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact
     8701 gatcgagaac gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta
     8761 tgatgatata tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg
     8821 aggaattaac gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga
     8881 agcaggtcat tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct
     8941 gctttggtcc aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac
     9001 caaaacttgc ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc
     9061 cgattgaatg gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg
     9121 gcaaagggtt agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct
     9181 tgttcaatgg gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca
     9241 agcgacatcc aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg
     9301 ggatggcagc aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg
     9361 ctgacgattc gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa
     9421 cactgatgtt ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca
     9481 tgaagaaaaa agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac
     9541 caattgatag ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat
     9601 tagcattggc cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga
     9661 tactcatgtc aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg
     9721 aaaatgaatt caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga
     9781 aaggaaatgc ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata
     9841 ttcactggtg gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata
     9901 gttatagttg cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc
     9961 ttaatgtttg aatttttttg gtgatagatt ttttcagccg tataaaataa atcgtaattc
    10021 tattatagag agttgtgatt ataaaaagat agcaagaaaa aaaaagaaag tttctataca
    10081 ttttaaatat tttattccta tttctattgc taaaaaagta gaaattctat atttaccttt
    10141 aactctcgct tctcttgcaa atgtagttag cattttaatc gataataaac tcaggtagtt
    10201 atattacctt gaaatgattg ataattttgt c
//
LOCUS       DACCWV010000099        10195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCWV010000099 DACCWV010000000
VERSION     DACCWV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.100"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..613)
                     /locus_tag="IVW84_002098"
     CDS             complement(<1..613)
                     /locus_tag="IVW84_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP7918281.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            886..3357
                     /locus_tag="IVW84_002099"
     CDS             886..3357
                     /locus_tag="IVW84_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7918282.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3460..5196)
                     /locus_tag="IVW84_002100"
     CDS             complement(3460..5196)
                     /locus_tag="IVW84_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7918283.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5193..5897)
                     /locus_tag="IVW84_002101"
     CDS             complement(5193..5897)
                     /locus_tag="IVW84_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918284.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6028..6531
                     /locus_tag="IVW84_002102"
     CDS             6028..6531
                     /locus_tag="IVW84_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7918285.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6491..6829
                     /locus_tag="IVW84_002103"
     CDS             6491..6829
                     /locus_tag="IVW84_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7918286.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6850..8268
                     /gene="ffh"
                     /locus_tag="IVW84_002104"
     CDS             6850..8268
                     /gene="ffh"
                     /locus_tag="IVW84_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7918287.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8380..8958
                     /locus_tag="IVW84_002105"
     CDS             8380..8958
                     /locus_tag="IVW84_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7918288.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8962..9483
                     /locus_tag="IVW84_002106"
     CDS             8962..9483
                     /locus_tag="IVW84_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7918289.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9562..10116)
                     /locus_tag="IVW84_002107"
     CDS             complement(9562..10116)
                     /locus_tag="IVW84_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7918290.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3287 a         1890 c         2107 g         2911 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga
      781 aaggtgtttg caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga
      841 gaagtttgga aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg
      901 ataaatcgaa gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt
      961 tttccatcgt ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt
     1021 gcagcaaata attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac
     1081 atatttacgt atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa
     1141 tcctataaga acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt
     1201 caggaaattc gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc
     1261 aatgtcatac gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac
     1321 attgttattt tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa
     1381 ctgcaagtta atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt
     1441 gatccattaa tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa
     1501 gttggagcag attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga
     1561 gaacagtcgg agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt
     1621 attcctatcg gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa
     1681 gaaagtgtcg ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg
     1741 ctgacgacga ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta
     1801 cacaatatga aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca
     1861 ggaacgatca cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa
     1921 ataatcagtc agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca
     1981 ggagacaatg caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc
     2041 tatcaccatg ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac
     2101 aaggtgtatg ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg
     2161 gaagagtttg tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat
     2221 gggattcttg aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt
     2281 gctaatccta tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta
     2341 gccatcaaag tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca
     2401 gggatttcac atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa
     2461 gcagctgtcg aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc
     2521 gttcaattga taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt
     2581 ctagccatga aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg
     2641 caggcctcac aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca
     2701 gaaggacggc gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat
     2761 attttctcct ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa
     2821 ccatcacaaa tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct
     2881 ctagaagaaa acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct
     2941 gtgccaggtg gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact
     3001 ttgaatctaa ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg
     3061 ttcctcgttc tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt
     3121 ttctgtgcta gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc
     3181 ggtatctcag ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt
     3241 tttaggcatt tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa
     3301 aagccgttta gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga
     3361 aaagtatctc aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg
     3421 agtcatccaa ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac
     3481 ggtgaaagtc gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg
     3541 cacgtagtct tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc
     3601 cttgtctacg cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat
     3661 accaaagtct tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc
     3721 gctgtcgctt tttgaatatt ggacagcatt ttcaatcaga tttttgataa ttggataaag
     3781 gatatctgtt ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt
     3841 cttttcgatg attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat
     3901 tgtctggatc tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag
     3961 tttatctaac cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg
     4021 gtcatctttt gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt
     4081 ttttaattcg tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac
     4141 tttcgttaag tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta
     4201 gcgcaaactc aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag
     4261 cggatgctca ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc
     4321 tgtgtaccgc tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt
     4381 aagcagtagt aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt
     4441 cagtaacgta tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg
     4501 ttcgctcaac gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat
     4561 tacttccgcc tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa
     4621 tggctggttt ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga
     4681 cagaaagaag aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaacgcttgt
     4741 cggttctgcg atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac
     4801 atataaaaga tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc
     4861 attcaatacg gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt
     4921 tgtgtcaaat aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg
     4981 gacgaattct tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg
     5041 ccggatcagc agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg
     5101 ttgaaaataa tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag
     5161 aaagaagaga cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag
     5221 ccaaatcctc taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct
     5281 cgcaaatgac taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa
     5341 atgcggtcta gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca
     5401 agtaattcaa attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa
     5461 ttggtcaaat cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt
     5521 gattgcttac gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga
     5581 ggactaaatg gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc
     5641 acttggtcat ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc
     5701 cgctgagtga tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca
     5761 aatgattgtt ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg
     5821 tagccttctt tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca
     5881 actagtattt ttttcattca aatctccttt aaataggttt cattctgttt atattttagc
     5941 atagaatcag tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact
     6001 ttgctgaata gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg
     6061 gcctgtcgct atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact
     6121 ggagaagctg tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc
     6181 gctgaaatca ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg
     6241 acaagcggcg aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa
     6301 ttgcaggaaa aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc
     6361 cattcgattt atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact
     6421 gcttatttta ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga
     6481 ggtgagcaga atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc
     6541 gaccttattg acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc
     6601 attaggtgag atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa
     6661 gcggacaagc aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat
     6721 tgttcgagaa caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga
     6781 taaaaaacta cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat
     6841 gaagaaaata tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata
     6901 agaaaaaaag gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt
     6961 gccttactag aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc
     7021 gaacgtgcgg taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa
     7081 atcgttgatg aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca
     7141 ccaaaaatac caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt
     7201 actggtaaat tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca
     7261 ggggacgtat atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac
     7321 gttcctgtat ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg
     7381 gagttagcaa aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat
     7441 atcgatgaaa cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag
     7501 attttgcttg ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc
     7561 aaccagcaat taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc
     7621 ggggcagcac tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt
     7681 gaaaaattga ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt
     7741 atgggggata tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg
     7801 gaagaacttg cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag
     7861 cttgatcaag tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg
     7921 aatcaagtcc ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa
     7981 gcgatggttt attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc
     8041 cgtcgtcgcc gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc
     8101 aaacaattca aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc
     8161 ccaggtatgg accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg
     8221 aatcgaatga tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac
     8281 agcttcggta cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc
     8341 tttactaata caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa
     8401 caaacagaaa gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac
     8461 caaaagctgt tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg
     8521 attttaaata aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc
     8581 gcaaattatg gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac
     8641 cgaagaaaaa tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa
     8701 atcggcagtt tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct
     8761 tatcattcac aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat
     8821 ctaaagaaaa gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct
     8881 gtcgggatca tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc
     8941 ggcagaaagg gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat
     9001 ggctatttgg caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag
     9061 caaactactt atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact
     9121 tatcaggaag caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc
     9181 gttttttcca cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct
     9241 gttgcttttc taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc
     9301 ggtcgattga tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt
     9361 acccctgtat tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt
     9421 tttgaaatgc tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag
     9481 tagaagtaaa taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct
     9541 tttttattac cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc
     9601 taaaatcgtt tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag
     9661 attcgcaacg tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac
     9721 aagtgattgg cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt
     9781 aacgactaaa cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc
     9841 taaagcattt ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac
     9901 accgtctagt cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg
     9961 agtttcgaga tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta
    10021 cccttcagga agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct
    10081 taaactacca ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat
    10141 ctataataat cttacaaaag ataagtaact ggagcaaata aaaacactta ctgac
//
LOCUS       DACCWV010000100        10011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCWV010000100 DACCWV010000000
VERSION     DACCWV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.101"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..107
                     /locus_tag="IVW84_002108"
     CDS             <1..107
                     /locus_tag="IVW84_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7918291.1"
                     /translation="SVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(285..2282)
                     /gene="tkt"
                     /locus_tag="IVW84_002109"
     CDS             complement(285..2282)
                     /gene="tkt"
                     /locus_tag="IVW84_002109"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7918292.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2384..2635)
                     /locus_tag="IVW84_002110"
     CDS             complement(2384..2635)
                     /locus_tag="IVW84_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7918293.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2782..3405
                     /gene="lexA"
                     /locus_tag="IVW84_002111"
     CDS             2782..3405
                     /gene="lexA"
                     /locus_tag="IVW84_002111"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7918294.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3585..3803
                     /locus_tag="IVW84_002112"
     CDS             3585..3803
                     /locus_tag="IVW84_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918295.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4092..4604)
                     /locus_tag="IVW84_002113"
     CDS             complement(4092..4604)
                     /locus_tag="IVW84_002113"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7918296.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4618..6915)
                     /gene="recJ"
                     /locus_tag="IVW84_002114"
     CDS             complement(4618..6915)
                     /gene="recJ"
                     /locus_tag="IVW84_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7918297.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7057..7491)
                     /locus_tag="IVW84_002115"
     CDS             complement(7057..7491)
                     /locus_tag="IVW84_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7918298.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7501..8289)
                     /locus_tag="IVW84_002116"
     CDS             complement(7501..8289)
                     /locus_tag="IVW84_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7918299.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8286..9227)
                     /locus_tag="IVW84_002117"
     CDS             complement(8286..9227)
                     /locus_tag="IVW84_002117"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7918300.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9404..9991)
                     /locus_tag="IVW84_002118"
     CDS             complement(9404..9991)
                     /locus_tag="IVW84_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7918301.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
BASE COUNT         2747 a         2235 c         1761 g         3268 t
ORIGIN      
        1 catccgttgc gtccaaaaga aaccatatcc ctagaaaatc actaaattac caaacaccat
       61 tagaagtttt tttgagttac gtaaatggaa agttttgtct cgcttaattt gacaaataat
      121 attctcaaga aacacaaact aatccgaaga attctttgtt ttgtcattta aatgtaacat
      181 agccgaaaac gaaaagtaat attgatttat tcttctaaca cataaagaac agctgtctcc
      241 aacttgcaga cagctgttct tatcagtcaa acaattgctt ggatctatga tagttgattg
      301 tacgtattca cgacattttc tactgtaaat ccaaattctt ctaaaatttt agtgccaggg
      361 gcagatgcac cgaaatgatc gattgtaatc gttgcgccct ctgttcctac gtagcgttcc
      421 catccaaatg atgcagcagc ttcgatagcc acacgtttct tcactgattt aggaagtaca
      481 ctttctttat attcagaaga ttgtttttca aagaggtcaa agcttggcat agaaacaaca
      541 gaaacatctt tgcctttttc agccaattct ttttgagctt tcacagccaa atcaacttca
      601 gaacctgttg caatcaaaat accttctggt gtttctcctt gtgatggaga aagaacatac
      661 gcgccttttt tcaccatttc atctgctact tcttttgtag aaggcaatac tggaaggttt
      721 tgacgactca atacaaggat agtaggcgca tctgttgtct ccatcgctac tttccaagca
      781 gcacgtgttt catttccgtc tgccggacga atcacttgaa caccaggcat gcagcgaaca
      841 gatgctagtt gttcaatcgg ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca
      901 tgtgttaaga cgtaaacaac aggagtatgt tggattgctg ctaaacgaac tgctggtctt
      961 agataatcga caaagacaaa gaatgttcca ccgtagatac gagttccgcc atgaagttgg
     1021 ataccattca tagccgaagc cattgcaaat tcgcggacac cgaaccagat gttgcgtcct
     1081 tcataatgtt caggagtgaa atctttatct gcagccacca ttgtattatt ggaacctgac
     1141 aagtccgctg aaccacccca gaagcttggt atagctttcg ataattcttg gatgacttct
     1201 ttacttgaaa cacggcttgc ttgactgctt ccaacttcat aagttggcaa ttctgcatcc
     1261 cagttttctg gtagtttgcc atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa
     1321 gcttttttat aattggcaaa catttcattc catgcatctt cagctttttg tccttcttct
     1381 atcatcgttt gatggaagcg ggcagctact tcttctggaa cagtgaaatc tggatactcc
     1441 caaccataaa cttctttagc catcttgatg ccatcttcac caagaggagc accatgaaca
     1501 gcagatgttc cttctttagg agcaccatag ccgatgactg tttttacttc gatcaatgtt
     1561 ggtttgtctg tttcagcttt tgcttcttca atcgctttag agattgcttc taggtcattt
     1621 ccgtctttca ctaagatatg ttgccagcca tatgcttcat agcgagcacc aacgttttct
     1681 gtgaaagctt ttgaagttgg accgtctagt gaaatatcgt ttgaatcgta taaaacgatc
     1741 aatttcccta gtttcatatg acctgccatc gaacttgctt cttgagatac cccttccatc
     1801 aagtctccgt caccgcatat tgcatacgta tagtgatcca tcacattaaa gttttctttg
     1861 ttatacgttg ctgcaaggtg cgcttctgcc attgccatac cgacagccat tgcaatccct
     1921 tgtcctaatg gccctgtagt ggcttctaca ccatctgtat gatgtacttc aggatgtcct
     1981 ggtgttttag aatcccattg acggaattgt ttcaaatcat cgattgtcac ttgatagcca
     2041 gctaaatgaa gcaagctata caacattgct gacccatgtc ctgccgaaag aacgaaacgg
     2101 tcgcggtcag cccagttttt tgatgtcgtt ggatttactt tcaggtgttt tgtccataac
     2161 gcatatgcca taggtgctgc gcccatcggc aatccaggat gtcctgagtt tgctttttgg
     2221 actgcttcaa tacttaatgt acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac
     2281 aaaataaccc ctcctattaa tttaggtttt ttttaccttt atttcttaca ctttcattct
     2341 ataagatgtc acacgaaaaa gcaatctttt tcagtatttt ttactaaaag ttgtcatttc
     2401 ggttgtgtaa gccttttttc ttttggattt cttttaattt ttccggggtc acgtcattgc
     2461 cctcaggatc gacgactttc attccttcga tatgatgacg cataccggaa cgaaaagctt
     2521 ccaaatactc tttgcgcaag ttgatttgtt cttgttcttc ggcttttgag agaccatttt
     2581 ccttcttttt acgtgcaagt tcattgatcc gctctatttt ttcaggtgac agcatgctca
     2641 gccctccttt tactctcctt catattatat aaaaactgaa aaaaaaacaa ctagataaac
     2701 taaataaaat acgaacattc gtttgattta gcgcttttta tatggtacta tttatttatc
     2761 aagtgaaaga aggtgtacct tgtggcccga caaactgaat caagacaaat cgaagtctta
     2821 cgatatatcc atgaacaagt aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg
     2881 gaagctgttc agctttcttc tacttctact gttcacggtc atcttgcacg gcttgaaaaa
     2941 aaagggttta tccaaagaga tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt
     3001 ttagaaaaga ttgggattcg tccaacaact attccagtac ttggcgtcgt aacagctgga
     3061 gaacccattt tagcagtaga ggatgcttca gacttctttc caatccctcc taatctttct
     3121 tccgaagaag gcagtctctt tatgctgaca attcgtggag atagtatgat caatgctggg
     3181 atattggatg gtgacaacgt gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt
     3241 gtcattgcaa tgactgccga ggacgaagcg acttgcaaac gattctataa agaaaaaaac
     3301 tttttccgtc tgcagcctga aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct
     3361 atattaggac gagtagtagg actttatcgt agtcatatct attaattgct actaattaaa
     3421 agaaaacttt ttaaaaagag tttgagacgt tggtctcaaa ctcttttttc gcccgggttc
     3481 ttcttctttc aaatttttat cacttccctc tttacaaacc gaacaaatat tcgtatacta
     3541 aatatacgaa caattgttcg cataaattaa agaggtgaga attcatgaaa tcaatgagac
     3601 gtcttggctt agtattaact gttttgtttt taggtattat tattggtaat ttcggcatac
     3661 gtccagcttt acttgtgttt gttccgttat ttactttgtg gttcatgttg tgggatgaaa
     3721 agaagtaccg tcaatctgag cggcgcagaa ttcatgaaga tcatttctat catgagccct
     3781 actacagatc caggcaatca taataaggat tgactgctaa ttattcaaaa gacaagaagt
     3841 taaactttta cgggggtgtg acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt
     3901 tctaaagcca gtttttcggt attaagtcaa attgcggaaa aagtgatttt ttaaacttct
     3961 actggtcttt gcttgtttcc atttataaaa aaatagcgac cctcaaaagt cagatagaga
     4021 atctgacttt tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt
     4081 cttttttctg cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt
     4141 ccatggagat ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt
     4201 tcgatcagtt cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc
     4261 agcacacgtt gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat
     4321 tctaatccat aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg
     4381 gcaaaaccga ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct
     4441 ggcccaacta ccatatcaat tcgtttttct tttgcataat ttacgatttg tttagtagct
     4501 tcccgataag cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt
     4561 gacggatagt cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta
     4621 ggttgttaac catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt
     4681 gattttattg agccgtcgct ggtataatct gctctcagtc aaaggatgac tttctgggct
     4741 agcgactttt tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg
     4801 tatcataaaa attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt
     4861 atgacgtata tcaattcgtt cttgagaatg gatcaattta aacaattttg cgtactgttc
     4921 tcttgtgcct accccattca aataggcctc atcaggtgaa atccccatca gataaacacg
     4981 attgaatgaa taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat
     5041 aacaactaga gagtttggtt ttcttttttc aattattgcc tttacagaat ctagtccttc
     5101 gaaaacaatc gcagcttcct ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa
     5161 tgctaaataa aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca
     5221 atcaaatact tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg
     5281 cccgttccat tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc
     5341 ttcttcttga gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg catcggaaag
     5401 gacaaatttc agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg
     5461 tcgaaataaa aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc
     5521 atcaattcgc tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg
     5581 ccctctcatc aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat
     5641 agtgacattt tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt
     5701 aaataaatct cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc
     5761 agatccttta gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc
     5821 attcattatt ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa
     5881 tagatggata tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac
     5941 gattgctttt ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc
     6001 ttcatcaaac gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc
     6061 atttagtctg ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac
     6121 gccgctttct tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat
     6181 tcctaatgaa acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc
     6241 tatcgctact aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt
     6301 gaatgctact ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg
     6361 gtgaacaatc gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat
     6421 cacgtctaca ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc
     6481 aacagtgatg atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg
     6541 tccataccca tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc
     6601 cagtgtttct ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac
     6661 gaggattttt tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc
     6721 gtgtatcaga tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc
     6781 cttttcctca tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag
     6841 ttcacggatc atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa
     6901 atttgcttgt ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa
     6961 aaaaagcaga aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac
     7021 ctgatctttt gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg
     7081 tttgggcgat tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt
     7141 cgcttgtaac tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg
     7201 ttgctgttct tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat
     7261 ttcctgagtc agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa
     7321 aaatacaatc agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga
     7381 aaaggaagtg aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac
     7441 aataatcaaa actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac
     7501 ctactttttg ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata
     7561 aaattttgat gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat
     7621 attgcggacg atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc
     7681 caaatagctt ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt
     7741 cactgtcgta acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc
     7801 aaggactgca aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc
     7861 catagaggcg atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt
     7921 ttgtgtcaaa accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag
     7981 gtcgaatgta aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac
     8041 gcgcccgact tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac
     8101 gtctaccttg aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc
     8161 ttctacaaat gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc
     8221 ataacagatt tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc
     8281 taaattcatg cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata
     8341 atcgtcgtgt tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct
     8401 aaatatctcg cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact
     8461 tcagcagctt gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta
     8521 ttaaatgtgc tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt
     8581 gtttttctcg tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa
     8641 tcttttccat tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg
     8701 ccgtacagtg gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct
     8761 ttatgatcat gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt
     8821 gtataaacag aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat
     8881 ttcagcggat aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct
     8941 atcccttttg gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg
     9001 atgagcccgg gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg
     9061 atttttctcg gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg
     9121 aacaaccaaa cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta
     9181 tgttttgcag gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact
     9241 ttctttaagt tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag
     9301 aatgggttca aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat
     9361 aaaaacagca gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag
     9421 aactttcatt gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt
     9481 ctttcgcatg attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat
     9541 ccaagcgagt aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt
     9601 cctcttcatc agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa
     9661 agtcgaacac ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc
     9721 ctccggcaaa ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc
     9781 cgattaatcc acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt
     9841 tgaattgatg tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg
     9901 aacgaacagc atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt
     9961 gaaaatttaa aatgattact cgtttgctca ttaaaaacgc ccctttcata c
//
LOCUS       DACCWV010000101         9898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCWV010000101 DACCWV010000000
VERSION     DACCWV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.103"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(119..571)
                     /locus_tag="IVW84_002119"
     CDS             complement(119..571)
                     /locus_tag="IVW84_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7918302.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(584..994)
                     /locus_tag="IVW84_002120"
     CDS             complement(584..994)
                     /locus_tag="IVW84_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918303.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1269..1481
                     /locus_tag="IVW84_002121"
     CDS             1269..1481
                     /locus_tag="IVW84_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918304.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1484..1696
                     /locus_tag="IVW84_002122"
     CDS             1484..1696
                     /locus_tag="IVW84_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918305.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(1988..2212)
                     /locus_tag="IVW84_002123"
     CDS             complement(1988..2212)
                     /locus_tag="IVW84_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7918306.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2290..2478
                     /locus_tag="IVW84_002124"
     CDS             2290..2478
                     /locus_tag="IVW84_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918307.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2804..3139
                     /locus_tag="IVW84_002125"
     CDS             2804..3139
                     /locus_tag="IVW84_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918308.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3372..4313
                     /locus_tag="IVW84_002126"
     CDS             3372..4313
                     /locus_tag="IVW84_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7918309.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4315..5205
                     /locus_tag="IVW84_002127"
     CDS             4315..5205
                     /locus_tag="IVW84_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP7918310.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5248..6048
                     /locus_tag="IVW84_002128"
     CDS             5248..6048
                     /locus_tag="IVW84_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7918311.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6063..6914
                     /locus_tag="IVW84_002129"
     CDS             6063..6914
                     /locus_tag="IVW84_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7918312.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7115..7576
                     /locus_tag="IVW84_002130"
     CDS             7115..7576
                     /locus_tag="IVW84_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7918313.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7602..8054
                     /locus_tag="IVW84_002131"
     CDS             7602..8054
                     /locus_tag="IVW84_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918314.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8160..8354
                     /locus_tag="IVW84_002132"
     CDS             8160..8354
                     /locus_tag="IVW84_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918315.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8385..8678
                     /locus_tag="IVW84_002133"
     CDS             8385..8678
                     /locus_tag="IVW84_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918316.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8708..9148
                     /locus_tag="IVW84_002134"
     CDS             8708..9148
                     /locus_tag="IVW84_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7918317.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9223..9690
                     /locus_tag="IVW84_002135"
     CDS             9223..9690
                     /locus_tag="IVW84_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7918318.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT         3524 a         1564 c         2045 g         2765 t
ORIGIN      
        1 ataaaataag tccaatcaat aataacattc aaaactcctc atttctttga tataattatt
       61 gtagagttta ctcagaaatg aggaaagagt ccatgtgaaa gcatgggctt ttttattttt
      121 aatgttgtaa tactaaatgt tcaagattat gaggaagccc taaatattca ataatttgga
      181 actgactcat atcagataaa tcttgttcat ctattgttct agttaacatt tcaattgcaa
      241 acatattagc ttcagcttct ataccataaa tcatacaact aaggttgttt gctctaaaag
      301 ctgctgtgca aataccttta tggagtctaa cgtgggctat ttcatgccat aatacaaatc
      361 ttttcataga aggtgatagg ttttgattaa ttaatatcac ataacatctt ttattgtgta
      421 tcgtttgtcc taatgtttta ttccctaaat cagtttcaat taaatctata tcagaatatt
      481 tgattatttt atatggatta atcgcatcag atccaaactc tacaaataat ttttctattt
      541 cttctaatat ccagtatttg tttaaatgca taccaaagcc cacctattga cgatattttt
      601 taggagtgaa ttttttcttt gctttaactt tggttaaggc aagtgcaagt tgaatggcat
      661 cgtataattt ttccttatct tcatcgtcta aaggttcacc ataataattt gtttcagcat
      721 ctgaattcaa gccttcaagt aatttttcag cttgtaaagc aatatcttgt ttatcttttt
      781 ctgttaaatc ataataatgg cgtttatcag tacgtcctaa aagataatca gttgatacat
      841 taaagtaatc tgcaactttt tgtattttat ctccagatgg agtactttta ttccatctag
      901 acatgcttcc atttccgaaa tttaactttc tttcaagttc tgccaacgtc attgaacgtt
      961 cttttaataa gatttttata cggtctaata gcataggata tgcattcctt tcaaaatacc
     1021 gacaaaaaaa gtatttttct gcaaattatc tatttacaaa tagagaattt gcagttatac
     1081 tagttttgta agcaagatat ttacggcata caaagactaa aagcaataaa ctaatagtct
     1141 ccccagacat cagtaagctc gctttttgtg cgctgtattt tgtatgctta cataatagca
     1201 tatttgcagt ttttgtcaaa cttattagac aaaaatatta gaaaatattc gataaggggg
     1261 tatgaagcat gactttaaaa cagcaagtat taattgcttt agtaaagaaa ggttggagtc
     1321 aaagagagtt agctcgacga atgggtatat caattactta tttacgagat atcttcatcg
     1381 aaaaaagaaa acctaaggaa cgtttaaaac aaatcgaaga acttttggat atcaagttag
     1441 aagtagattc taatgatcag ccatcagaac aggaggcgta aaaatggtac aacgtttgag
     1501 caaagcggag tttaaagaga aatataagta ttctgaaagt acttatcaaa gaagaatgaa
     1561 ggaatttaaa aaatcacgtt ttagtgaggg gtataacgca gttacttcca aagaaataac
     1621 aattgatgtc gatttatatg aacagtttag aacatggcgt tctaaaaatc gatttagagt
     1681 tagagctgtt tgttaatagc taaaaaatct aaattcacca cttctgaaaa gaggagttgc
     1741 tgctctcctt tcaagtttgc caaagtccac gttttttcat tttggcaact cctcttcttg
     1801 gaggtggtga cggcgagaga cgaaggaggg atgacatgaa ggaagaatca aaaataggga
     1861 ttttgaatga gaacaattac acaagattaa ttattgaaac agaagaaggg aaaaaagtag
     1921 cagaagttac attgaccgat gcaactcctg ctgacggtta tcgtattcga ttaactccta
     1981 aatatgatta accttttggt gggtgtggat cgtttccgtg actatcacgg ctttgaattt
     2041 ttccatctct gccatgaata aatagctcag atccttgatt tttagaaatc tctctagcga
     2101 tttttactgc ttctgatttt gtattagtat gtgcagtcgc tttagaattt ccagcacctt
     2161 tgacattcca accaccattg gaattaggta caacatgttg attttttccc atagttttca
     2221 cctccatttc tatagaaata ttttatcaaa tagaaatgga gaaattcaat aaacgaaagg
     2281 agaagtgata tggcatatac cttgcaacaa gaacatcaaa ttctcggttt gattaaacaa
     2341 cgcaggaaac aattacaaga tgaccgtgca gcgcttagaa aagccgatga gctatcagat
     2401 aggcaagctg aactaattgc ttctgaactt gaggatttga gaatgctaga aataaaaaat
     2461 agggagatta gattatgaag aagacagaca cactttttat aggattcatt ttggggttat
     2521 tagtgattgt agcgcaccaa agtattatcg ggggaagctt gttcgcagca ttgatggttt
     2581 taatcaatct gcttgattca aaagaaagga gcaactatgg cacgagaaga agcgctaaaa
     2641 atcggtaaag tgattgctga taatcggtgg ggaaatagcc gccctattat tttaagcaag
     2701 caacatatcg aaaagcaaaa agcatggcaa caaataaaaa agtgactctg ccagccagca
     2761 aagagtcaca tacaaattta taaaactagg agaattatat cacatgaata agaaaattga
     2821 aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa ggtgtttcta ttatttgtac
     2881 agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt gagacaacag aaattttact
     2941 ttgtcttgct atgcaagaag aacatttaga tgaaaatctc ccattgtcag cacatattat
     3001 gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat gaagaagaaa atcaaccttc
     3061 aaatcatact tttgttatta ataacaagga agatttagct gatgtgatga ctcgtattct
     3121 caagggggaa tttcaatgat gaatgaaaaa acaattgatc tactcattat tttatttttc
     3181 acgttagtga ttgtagcgaa aagttatgta atctttggct tgttattcgc aattaccatg
     3241 cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac atgaacgagt tagcaaatct
     3301 tgacaactat ttaactgatc ctgattatgc taaaccacca tatgaagcac caattgatga
     3361 ggaggatgaa gatgagtaaa tctaccttag aaatgagcca tcaagaatgg cttgaagacc
     3421 gtaagaaagg catcggaggt tcggatgttg gaacaatttt aggattgaat aaatggaaat
     3481 ctccttatca actatggcta gaaaaaacag gacaagtagt acttgaagaa tcaggaagtg
     3541 agcccgctta ttggggtaat gttttagaag aagtggttgc taaagagttt caggaacgta
     3601 caggcaaaaa agtacgcaga agaaaccaag tatttgaaca tccattacat ccatttttaa
     3661 gagcaaatat tgatcgggaa gtagtgggag aaaatgccat tctggaatgc aaaacagcca
     3721 atcaatttct cggtaaagaa tgggaaggcg aagaggtacc gcttagctat ctctgtcaag
     3781 ttcaacatta catgaacgtt ctaaacaaag actattgtta catcgctgtc ttaatcggtg
     3841 gtcaaaaatt tatctggaag cggattgaac gagatcaaga gctgatcgat acaatcactg
     3901 aacaattagt agagttttgg gaaacaaacg ttcttggagg tatcgagcca attattgatg
     3961 gtagtcaagc gacagctgat ttcttgaaag agaagtatgc agatgttgaa gacgttcaaa
     4021 cgacgttacc aattcatttc gatgaactag ttgaacagaa aaacgaactc aaacggacta
     4081 agaaagaaat tgaatcagct atccgtcaag tagacaacga gattatcagc gagctaggta
     4141 aacgtgaagc cagtatcggt atcactcaaa aaaacatcat cagttggaaa tttgtccgta
     4201 cgagacgtat gaactcgaag aaactagcag agaaatatcc agatgtcgca aatgatgaag
     4261 agatttataa cgttactgaa tcaagaaggc taaccgaaag ggagatcaaa taatatggca
     4321 acaaatgaat cgttaaaaaa tcaattggca gcaaagccac agaaacaagt tgcaccaggt
     4381 caattaggac ttaaagcttt gatgaataca ccaacaatga gaaagaagtt cgaagaggtg
     4441 cttcatgaca atgctaatgc ttttatgtcg aatgttatga ctcttgtatc taatgacagt
     4501 tatcttgcag atagtgaacc gatgtctatc atgagtggtg cgttaactgc tgcaacatta
     4561 aatcttgggc tagataagaa tttaggttat gcatatttag ttccattcaa tagtaaaaac
     4621 aagcaaacag gaaaatggga aaagaaagct caattcatgc ttggctataa aggatatatc
     4681 caattagccc aacgatcagg taaatacaaa gcattaaatg tgatcgaagt ttacgaagga
     4741 gaactaaaaa gctggaaccg actgacagaa gagtttgagt ttgatccaaa tggtagaacg
     4801 tctgatgaag tcattggata tgttggctat ttcgagttac tgaatggatt caaaaaaact
     4861 gtctattgga ccaaacaaga aatcgaagct catcggattg ctaacaataa agatcgcgat
     4921 aagacaaagt taagtggtgt gtgggcatct gattacaatg caatggcacg aaaaactgtt
     4981 ttgagaaatc ttctttctaa atggggaatc ttgtccatcg aaatgcaaga agccaccaca
     5041 tcggatgaga gagtccaaag ggttcaagaa gacggcagca ttattgctga aacagaagtt
     5101 gaagaagata ttcctgaaag aaaagaagca gaggttatat ctgaagaaaa tgaagatgta
     5161 caaactggat tatttgatgc atctaatccg ccgttaaaca aataatgagg gagctttctc
     5221 ccttgctttt ctagaggaga aatacgaatg aacacaggat atgtcaaatt acatcgaaaa
     5281 gtgatgaatt cattcgtttg gaccaatcct tatatgtata aattatggaa cttgtgtttg
     5341 atgaaagcga gtcatgagaa tcgcaagata ctttttaatg gaaaagagat acaagtgaac
     5401 agcggagaat tcgtcacagg gcgcgatgct atcacatctg agatgaacga aggcgtgaaa
     5461 cgtgaacatc aagtgaacag cggttctgta tggagatggc taaaacaatt tgaaaaaaac
     5521 ggaatgttga acatcaaatc aactacgaaa tacagcgtta tatcaataaa aaactggtct
     5581 ttgtaccaaa gcagtgaaca acaaatgaac atcaaacgca caacaagtga acaacaagtg
     5641 cacacaaaca agaatgaaaa gaattataag aatgaaaaga ataataaaac atcatcgtcg
     5701 caaccacgca aaaagcgtgt ttacgacacc gactcagttt actacattct cgcggaggag
     5761 ttattcaaac agatttgtca gaatcaggaa atcaaaaagc cgaatctgca aagttgggca
     5821 gatgacattc ggaaaatgat cgagatcgac aagcggaccg aaaaccaggt acggggaatg
     5881 attgaatgga gtcagcacaa tgtgttttgg gcatcgaata ttttatctgc taaaaaattg
     5941 cgagaaaaat acgacacaat ggcagcgcaa gcaaatcgtg attataaaac aaaacaaact
     6001 aaaacgcttg aatacgagaa atttagcaca gatgagttgc ctatttgaga ggaggcgtag
     6061 gcatggaaac tgttggggaa atcatggaaa agctgataca gaaagtgctt gtccaacgtg
     6121 gcgaatgtcc tgaatgcgga cagcctttgt atggatggcg tacgaagaac cctgatggct
     6181 cagaacgttg taagccaacc tgtatgcaat gtgggtataa agcattgcga gttcaggaag
     6241 acttacaaac agaacgaatt tacaacgaga gcctgaaagc aagagcaatc aattttttca
     6301 aaggtggttc tgttgtgcct aatcaagcgt tgtttgattg cacattgcag aattatcaaa
     6361 ttgtcgatca agaaacaaga caagcggttg aagtaaccaa acgctttgtt aattcagtct
     6421 tgttaggaaa tccaagtcac cttgttttaa ctggaaaaca aggaacaggt aaaagtcacc
     6481 tagcgatggc agcggcttgg gaagtcttga agcgatcaaa ctacgataaa aaaatcttat
     6541 ttatcgggtt acaggaaatg ctggatcaaa tcaaattttc gtacaacaat cctgaactca
     6601 gaaaaacgat tgagggatcg ttgattgcag atatcaaaac agcggattta gtcatcattg
     6661 atgatatcgg ttcagaacta ggaaaagatg catcagatag tcgagcgttt ggcataaaca
     6721 cgctaaattc gttcttggat gcacgacaga acctagcaac gattatcaca acgaacttgc
     6781 ttggtgaaga actgaaaaaa gcttacggta cgagaacaat atcaagaatg tttgtcaact
     6841 ctgatggatt tacgatggta ttttctcaaa cagcagacaa gcgcataaaa ccagtcaaag
     6901 gagcgatcgc atgaatttga ctaaacaaga aaaaagaatc tgtcgtcaga taatgttgca
     6961 aattataggt gatcgtgaca tggacgaatt atctcaacaa gagagtcgaa tggttcggaa
     7021 gataagtaag ctaattattt atcgggatac agaaagattt ctcactgttt taggtaacgt
     7081 ctacgagaat ccagagttat tggaggtaga gtaattgcca aaaatattag atgcatgttg
     7141 cggtagtaga ttgttctggt ttgataagaa caattctgac gtaacattta tggattgtcg
     7201 tcagcagtat gaagaattat caacaggtca tgtgatcaat gttgatccag atgtggtcgc
     7261 agactttcgc gatatgcctt ttgaagatga tgtgttcgat atggttgtat ttgacccgcc
     7321 acacttaaga catgtcggcg aaaactcatg gcttgccaaa aaatatggaa aattagacga
     7381 actttggcca gaagacatca ggcaaggttt tgcagagtgt atgcgagttt tacgaccttc
     7441 aggaacatta atttttaagt ggaacgagga acagatacca ctttctgatg tgttagaggc
     7501 tattggagaa cagccattgt ttggtaataa acgaagcaaa acacattggc ttgtgtttat
     7561 gaaaaaattc agctaacgga ggaaacaaag gaggaaaagt aatgaagatt gtattaaata
     7621 aatgttttgg tggctttggg ttgagccatg aagcaaaaat ggaaattttt aaaagaaaaa
     7681 atattgaagt attcccatac atcaataact ttagctatga ctcagatgat gaatacacga
     7741 gatacacagg ccaaaagcta ggttcgatgg attttatcta ctatttcaaa aaagacccga
     7801 aaatcgacaa agttacagga atttatagcg aaattgaccg actgtacgga attgctgatg
     7861 attccagttt ttcaagtgat tcaaacagag gtgataagga cctcgttgct gttgtcgaaa
     7921 aactaggaag tgaggccagt ggaccatatg caagtcttaa agtcgttgat atacccgatg
     7981 gggctgagtg ggaaataagt gattatgacg gcgttgaaac tgcacattat ggttttcaaa
     8041 caggtagtat ttagtccact aatgacaact aaagaggagg cagaaggatg agtgaagatg
     8101 aatactttga acagtagtct attaacgaca gagattttta actggaaagg ggtgtagtca
     8161 tggacgcatc ggaagtagta aataaaatta atagtctcaa agaggttttc ggagacgtcg
     8221 aagtagtgat aaacgcacaa ggctatgaca acgaattttt taagcagatt accgacatta
     8281 gcattcagca aggaatggaa gacgaagacg gaaactttat cgatgaagta gccattttag
     8341 tgacttgcga gtaagtaatt gacagctaaa gaggaggaag cggaatgaaa ctaaaagacg
     8401 gattttactc cagcggccac ggcatcggcg gtttaatgct agatatgccg acaaagaatc
     8461 ctaaaacacg taagaaacca aaattcaaag tcggtgacat ggttcgctgc gaagcagaag
     8521 gattcatcta tccatttcgt ggatatgtag aacacgtcta taatcactca gcaatcattc
     8581 gcattgaaaa cacgatggaa tgtgataagt ggttagcgaa aagcaaagag aatttagcag
     8641 tggtgagatt ggtggacatg gaagttataa aatcttaatt ttgctttttt acaactatcc
     8701 tacaacaatg agcgttattc cacaactgga aggagaagag gcaattgtga acattttaga
     8761 gaatatagat attaagcaaa caaggaaaaa tgctagacga ttactaaaaa gatacagaaa
     8821 tttagaacgt ttggtcggac cagtgaaaat agacttttcc gtgatgactg ttaccaaaaa
     8881 tttgaaattc acaattgaca gtcaaaacga agaaatcatt gaagcgataa gtactcgaga
     8941 ttcggttatc gaagcattaa cacggctaag tagaatccat ttccaggtac tttattatag
     9001 ttattgtttt cctaataaga tgtcaatgta tcaaatagga gaaaaattag gatattccga
     9061 tagaactatc gagagaatga aggcagtagc tttggttgaa tttgctgagg cgtataaatc
     9121 aggagaactc atttcacgta caaaataaaa aaagtcggat tcctccgact atgaataata
     9181 tttccgacat aagtattata tcataattgg gggaatcaga ggatggtact tttcgatgta
     9241 aagaaatatg agacaccaag cgcgaaagat gttgatatgg aacgcacaaa acataatgtc
     9301 gctgtgtttc tttcagcata tctatcagct agatgtagag tagggcaaca tcgagaacca
     9361 aaagtgacag catcttattc cttggttcca ccttctacag ctgatcatat atttgaagca
     9421 gaaagaatgc tgatcgataa agaagaagca caggaagaat ttgagtatct gcataaattg
     9481 tttgttcgag gctattcagc gatacagcat ccgcataagc ctgatgtgac tgaaaggcgc
     9541 aagaagatat tctatgatcg ttatatcaat ggtctgccca tttatgtaac tgctcaaagg
     9601 aataatacta gcgaagaatc ggttaaagta gaatcaaaca gaattatcat ccaatttgct
     9661 tcatcgttag aactggttgc tttcaagtag ccagttttta cactttttat acccttttat
     9721 taccaatttg gtttccattt tatacctttt ttataccaat cacttaccta ttcaacgttg
     9781 tattatgata gtgtcgaaaa attaggaaac aggatcgaca aaataaaatg taagggagga
     9841 aatctccctc atcgtttaat taagcttcga taggcagcaa cggaaatatt aagaataa
//
LOCUS       DACCWV010000102         9825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCWV010000102 DACCWV010000000
VERSION     DACCWV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.104"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(79..2199)
                     /locus_tag="IVW84_002136"
     CDS             complement(79..2199)
                     /locus_tag="IVW84_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7918319.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="IVW84_002137"
     CDS             2429..3607
                     /locus_tag="IVW84_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7918320.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="IVW84_002138"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="IVW84_002138"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7918321.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="IVW84_002139"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="IVW84_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7918322.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="IVW84_002140"
     CDS             complement(4960..6213)
                     /locus_tag="IVW84_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7918323.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="IVW84_002141"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="IVW84_002141"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7918324.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="IVW84_002142"
     CDS             7790..9136
                     /locus_tag="IVW84_002142"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7918325.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..>9825)
                     /locus_tag="IVW84_002143"
     CDS             complement(9444..>9825)
                     /locus_tag="IVW84_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7918326.1"
                     /translation="KILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAETD
                     IKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKASEK
                     RIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3136 a         1788 c         2010 g         2891 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa ttttt
//
LOCUS       DACCWV010000103         9796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCWV010000103 DACCWV010000000
VERSION     DACCWV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.105"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(199..1182)
                     /locus_tag="IVW84_002144"
     CDS             complement(199..1182)
                     /locus_tag="IVW84_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7918327.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1187..1834)
                     /locus_tag="IVW84_002145"
     CDS             complement(1187..1834)
                     /locus_tag="IVW84_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7918328.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1919..2551)
                     /locus_tag="IVW84_002146"
     CDS             complement(1919..2551)
                     /locus_tag="IVW84_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7918329.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2567..3118)
                     /gene="coaC"
                     /locus_tag="IVW84_002147"
     CDS             complement(2567..3118)
                     /gene="coaC"
                     /locus_tag="IVW84_002147"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7918330.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3115..3852)
                     /locus_tag="IVW84_002148"
     CDS             complement(3115..3852)
                     /locus_tag="IVW84_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7918331.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3849..4838)
                     /locus_tag="IVW84_002149"
     CDS             complement(3849..4838)
                     /locus_tag="IVW84_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7918332.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5096..5638
                     /locus_tag="IVW84_002150"
     CDS             5096..5638
                     /locus_tag="IVW84_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7918333.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5655..6740
                     /locus_tag="IVW84_002151"
     CDS             5655..6740
                     /locus_tag="IVW84_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918334.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6752..7558
                     /locus_tag="IVW84_002152"
     CDS             6752..7558
                     /locus_tag="IVW84_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7918335.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7559..8386
                     /locus_tag="IVW84_002153"
     CDS             7559..8386
                     /locus_tag="IVW84_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7918336.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8383..9456
                     /locus_tag="IVW84_002154"
     CDS             8383..9456
                     /locus_tag="IVW84_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7918337.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2969 a         1806 c         1997 g         3024 t
ORIGIN      
        1 tcggaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
       61 taaacggcgg aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa
      121 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
      181 tttttgatca gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt
      241 tgcttccata tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat
      301 tggccgaatc ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag
      361 ctctgcttta tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt
      421 cccaatcaat acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa
      481 gacccaacgt ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat
      541 gaaatccaaa ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa
      601 gtcttgtttt ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt
      661 ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat
      721 cgctgctgtt cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa
      781 ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc
      841 agaaacatag tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata
      901 ttcggcataa ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga
      961 gatatcactt ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg
     1021 aggtttcaaa gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg
     1081 gactttgatc agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa
     1141 tacatctaca gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg
     1201 cccattcttg gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac
     1261 gagaaatatc cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga
     1321 tcgtttttga tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt
     1381 tagtcaatgt gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat
     1441 ctgcataatg tttagtaata taataatctg tcatggactc aggccatttt gctggttcat
     1501 ctgcataaac agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca
     1561 ctgttttgat tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag
     1621 caaaaatcaa tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta
     1681 aatcaaaaga gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc
     1741 tcacccctgc aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa
     1801 tttgtccgtt tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc
     1861 tttccattca atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc
     1921 agttagtaag agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg
     1981 caagacctgc gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg
     2041 aataaacagc atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt
     2101 agatcatatt catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata
     2161 aagaaacgga atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag
     2221 gaaccacccc aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat
     2281 gttctgttcc gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa
     2341 ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg
     2401 gtcctaaaac gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac
     2461 cgatagggat aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg
     2521 cagttaatgt aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt
     2581 cattaagagt cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc
     2641 caaaatctcc acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc
     2701 tcaatgtttc cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag
     2761 gggcaatcaa ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt
     2821 ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca
     2881 agtcagcttg ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga
     2941 cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg
     3001 tgctgctttt agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa
     3061 gatcagcgct tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt
     3121 tgattcctcc agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc
     3181 ttcttccact gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc
     3241 cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac
     3301 tgccagaaga gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt
     3361 tggctgccat tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag
     3421 atgctcggtg ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa
     3481 ccaattgtcc agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc
     3541 tgaaaaacta aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta
     3601 gtgttctttt ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt
     3661 ataaatcgtt atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc
     3721 gattgtggta tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt
     3781 cgaatgattt gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat
     3841 tagaatcttc atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca
     3901 agtagtttag atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca
     3961 tctgcaatct gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca
     4021 ttgacagaat caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg
     4081 tttagtttga ttcccccagc attgccacat aaagctactg caccgttata agagatcaat
     4141 ggaagaagac tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt
     4201 tttcctaaag cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca
     4261 cctagacttt ttagccatgt gacagactct tcttttctgg aaaaggtaat gattgactgg
     4321 aatcctagtt tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta
     4381 acgacaactt ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata
     4441 gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga
     4501 ggaactaacc agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg
     4561 ccaagcccaa aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt
     4621 tggacgacag ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga
     4681 ccagactcgc tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc
     4741 aagacttcat tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca
     4801 ggttccttta ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa
     4861 ttttctttta tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac
     4921 aaaaattagg tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa
     4981 tattcagcaa aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata
     5041 cgagtggtag taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga
     5101 aattggtgag aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg
     5161 cgaacgcaca gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc
     5221 atcaatggaa acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt
     5281 tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt
     5341 cgatgaagaa aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat
     5401 ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt
     5461 atctgaaacg tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt
     5521 ctatgtggca aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag
     5581 gaatcatctc aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata
     5641 tagggggtaa aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg
     5701 atgatgaaac agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac
     5761 tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg
     5821 aagctacaga aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata
     5881 agcgacaagt aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg
     5941 ataacgttgc ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg
     6001 taaaagatgc tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa
     6061 tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag
     6121 tattgctatt agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt
     6181 atgaactgcg ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc
     6241 aagaagaagc gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc
     6301 aaagtggtac acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg
     6361 ttggggagag caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg
     6421 gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt
     6481 tacgcccaga ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg
     6541 atactcagct gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg
     6601 aatggatggt ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg
     6661 agccagaaga tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc
     6721 ttgacagcta cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt
     6781 cccttatatt ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca
     6841 atcttttttt gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc
     6901 ggggacatcg ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt
     6961 cacgctgatc atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt
     7021 atggctgatg ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt
     7081 tattgggatt ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg
     7141 gccgaaacag atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat
     7201 tccatttatg attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag
     7261 tgctagtaga gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt
     7321 atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt
     7381 catgctgact cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca
     7441 gcatttcttg gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat
     7501 tgctatgttc atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat
     7561 gaaaaaattt aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc
     7621 gatcttctat ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg
     7681 atttacttgg gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt
     7741 gaatacgttc atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg
     7801 ggctatggga atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa
     7861 taacatctta ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac
     7921 actactagga ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat
     7981 tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga
     8041 tgcggctaga gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta
     8101 tctttctcca ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt
     8161 tgcggttaca tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc
     8221 tcgtgcaaga caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt
     8281 ctccatgata ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct
     8341 gaagaaaaac agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct
     8401 ctgtttttag gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa
     8461 aaaacaagtg gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc
     8521 gatcctagtt tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca
     8581 tttgattcta atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc
     8641 gctataccaa gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta
     8701 gatcattcga agatcaaagg attaaataat attgatccac gatttttaga cttggatttt
     8761 gatccagata atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac
     8821 gataaattct ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta
     8881 aaagacagtt tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt
     8941 ttaggctatt ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg
     9001 aatcatttaa caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc
     9061 aatgaagaag cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa
     9121 aatgaacatt tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc
     9181 gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta
     9241 gaaccggaaa atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa
     9301 gcaaaagcgt tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac
     9361 acgatctccc atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat
     9421 gacctattct tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg
     9481 acttatcaac gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt
     9541 ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt
     9601 ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa
     9661 tctaagattg aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa
     9721 cccttctgta atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg
     9781 aaaaaacata aaaatc
//
LOCUS       DACCWV010000104         9651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCWV010000104 DACCWV010000000
VERSION     DACCWV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.106"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            185..1183
                     /locus_tag="IVW84_002155"
     CDS             185..1183
                     /locus_tag="IVW84_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7918338.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVW84_002156"
     CDS             1295..1804
                     /locus_tag="IVW84_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7918339.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVW84_002157"
     CDS             complement(1834..2493)
                     /locus_tag="IVW84_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7918340.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVW84_002158"
     CDS             complement(2495..3259)
                     /locus_tag="IVW84_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7918341.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVW84_002159"
     CDS             complement(3267..3944)
                     /locus_tag="IVW84_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7918342.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVW84_002160"
     CDS             complement(3981..5366)
                     /locus_tag="IVW84_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7918343.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVW84_002161"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVW84_002161"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVW84_002162"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVW84_002162"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7918344.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVW84_002163"
     CDS             6305..7162
                     /locus_tag="IVW84_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7918345.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVW84_002164"
     CDS             complement(7243..7578)
                     /locus_tag="IVW84_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918346.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVW84_002165"
     CDS             complement(7645..9015)
                     /locus_tag="IVW84_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7918347.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVW84_002166"
     CDS             complement(9093..9629)
                     /locus_tag="IVW84_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7918348.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2825 a         1992 c         1619 g         3215 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgata tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca t
//
LOCUS       DACCWV010000105         9463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCWV010000105 DACCWV010000000
VERSION     DACCWV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.107"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            11..538
                     /locus_tag="IVW84_002167"
     CDS             11..538
                     /locus_tag="IVW84_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7918349.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="IVW84_002168"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="IVW84_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7918350.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="IVW84_002169"
     CDS             2005..3162
                     /locus_tag="IVW84_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7918351.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /locus_tag="IVW84_002170"
     CDS             3332..4792
                     /locus_tag="IVW84_002170"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7918352.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            5114..5650
                     /gene="cysE"
                     /locus_tag="IVW84_002171"
     CDS             5114..5650
                     /gene="cysE"
                     /locus_tag="IVW84_002171"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7918353.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /locus_tag="IVW84_002172"
     CDS             5647..7059
                     /locus_tag="IVW84_002172"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7918354.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="IVW84_002173"
     CDS             7056..7451
                     /locus_tag="IVW84_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7918355.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="IVW84_002174"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="IVW84_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7918356.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="IVW84_002175"
     CDS             8308..8838
                     /locus_tag="IVW84_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7918357.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..>9463
                     /locus_tag="IVW84_002176"
     CDS             8915..>9463
                     /locus_tag="IVW84_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7918358.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIK"
BASE COUNT         3231 a         1724 c         2136 g         2372 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actcaaaata aaa
//
LOCUS       DACCWV010000106         9431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCWV010000106 DACCWV010000000
VERSION     DACCWV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.108"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..680
                     /locus_tag="IVW84_002177"
     CDS             <1..680
                     /locus_tag="IVW84_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7918359.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="IVW84_002178"
     CDS             800..1000
                     /locus_tag="IVW84_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7918360.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="IVW84_002179"
     CDS             1076..1273
                     /locus_tag="IVW84_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918361.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="IVW84_002180"
     CDS             complement(1570..3117)
                     /locus_tag="IVW84_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7918362.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="IVW84_002181"
     CDS             complement(3201..4004)
                     /locus_tag="IVW84_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7918363.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="IVW84_002182"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="IVW84_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP7918364.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="IVW84_002183"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="IVW84_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP7918365.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="IVW84_002184"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="IVW84_002184"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP7918366.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="IVW84_002185"
     CDS             complement(6389..7318)
                     /locus_tag="IVW84_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7918367.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="IVW84_002186"
     CDS             7513..8196
                     /locus_tag="IVW84_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAP7918368.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9399
                     /locus_tag="IVW84_002187"
                     /pseudo
     CDS             8357..9399
                     /locus_tag="IVW84_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
BASE COUNT         2892 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaga ttttacgtat
     8581 atagaaacga acaatccaga ggataacagt accaatatcg atcaggcaat cagaaacggc
     8641 tttaccacca ttattagctt gagctacttg caagcagagg aactggaaaa tgctgctaaa
     8701 gataatccaa agttgaaatt tgccttggta gatggtgaga ttactgggaa aaagaatatc
     8761 tcatcagcaa cctttaaaga tcaagaaagt gcgtatttag caggactagc tgccgcatat
     8821 acgacaaaaa gtgatcaagt aggctttatt ggtggaatgg aagggcccgt gatccaacgt
     8881 ttccaaagcg gatttgaagc tggcgttaag gcaggggcca cagcactgaa caaggaaatt
     8941 actgttaacg tacaatatgc tgcatcctat gattcgctag ataaaggaaa agctattgct
     9001 gcttctatgt acaaagaggg agtcgatatt ctttatcagg cggcaggaag tactggagct
     9061 ggggtgttcc aagaagcaaa agcccgcaat gaaaacgact atagcagaaa agtatgggtg
     9121 attggtgtag atcgcgatca agcggatgaa ggggattata aaatggagaa cggcgagaca
     9181 gggaatttca cgttgacttc tacaaagaaa aatgtcggtg cagaagtcaa agaatttgca
     9241 gaaacttctc ataacgataa atttcctggc ggcaagcatt tgacctttgg tttgaaagaa
     9301 gaaggggtag atctgacaaa aggacaatta tcagaggaag cctatcaggc agtttcagac
     9361 gcaaaagaga aaatcatcag tggtgatttc agactgtaga caaagtccat tttatggatg
     9421 atgtctacag t
//
LOCUS       DACCWV010000107         9348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCWV010000107 DACCWV010000000
VERSION     DACCWV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.109"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(137..523)
                     /locus_tag="IVW84_002188"
     CDS             complement(137..523)
                     /locus_tag="IVW84_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7918369.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(520..1041)
                     /locus_tag="IVW84_002189"
     CDS             complement(520..1041)
                     /locus_tag="IVW84_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7918370.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(1043..2077)
                     /gene="sppA"
                     /locus_tag="IVW84_002190"
     CDS             complement(1043..2077)
                     /gene="sppA"
                     /locus_tag="IVW84_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7918371.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(2110..3525)
                     /locus_tag="IVW84_002191"
     CDS             complement(2110..3525)
                     /locus_tag="IVW84_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP7918372.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3494..5647)
                     /locus_tag="IVW84_002192"
     CDS             complement(3494..5647)
                     /locus_tag="IVW84_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP7918373.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5905..6141
                     /locus_tag="IVW84_002193"
     CDS             5905..6141
                     /locus_tag="IVW84_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7918374.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6160..6375
                     /locus_tag="IVW84_002194"
     CDS             6160..6375
                     /locus_tag="IVW84_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7918375.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(7078..7830)
                     /locus_tag="IVW84_002195"
     CDS             complement(7078..7830)
                     /locus_tag="IVW84_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918376.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7845..9092)
                     /locus_tag="IVW84_002196"
     CDS             complement(7845..9092)
                     /locus_tag="IVW84_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7918377.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(9168..9314)
                     /locus_tag="IVW84_002197"
     CDS             complement(9168..9314)
                     /locus_tag="IVW84_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7918378.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         3056 a         1652 c         1308 g         3332 t
ORIGIN      
        1 tcgaaaaaat tagaataaat aaaagacacg tagttttttt atctacatgt cttccatcga
       61 ctacaagtta tcatttctca aaacgtattt agcttttaat tgattaatcg tttcacgttg
      121 tctacacatt gcctaattaa gattttctta ttagaagcat caatcccgca ataataccta
      181 aaactccagg aattacccac aataatgtta aaagaaaaaa tggaataata gataaaatga
      241 gtaaactaat accatagacc actgtttttt gattttttct tactaaaaaa cctattatga
      301 aaatcattat cgaaaaaact agggtaattc ctccaaataa aacaacattc ccgcttagat
      361 tgactaacat atctatatcc ttactggtta gttctgtagt atttaaagta gttgaatcaa
      421 tagaagatag aaacatacca cccatcaatg ctaaaagact acataaaatc cctgttattc
      481 cactaattaa aatcaaaata aactctgttt ttctcttatt catttaattc tctccagatt
      541 aagtgtttta gaattagaaa ttttttgacc atagttaata gcttctaatg aacgaataac
      601 aaccactgac gtgttagaaa aataatcccc aatatgttgt ttcttgggcg tgaaagcagc
      661 caccaaatac ccaatttgaa atacaaaatt ttttaatata aacctacata tacactcacg
      721 aataaagata gtttgccaac ttagctcttt ttcctcaaag ctcactactc ttatcccaaa
      781 aatcattttc ccaattgttt gtcctgaatt cagcttagtc aaaagtacaa aataggttaa
      841 ataaatagct aatgacgcta taccataaaa agtaataatg gatttgtcag attccagtcc
      901 catgattttg taaaagagac ctatagtagc atgggtgatg atgctaatac aaattaaatc
      961 aataagaaaa gcaaaaaatc gtaaccaaaa tcccgcaaat acataattag gcaaagtttc
     1021 aatgccactc ttcttattca ttttatagcc ctccatagta atacattggt tttggagtat
     1081 cagaaaatcc aagtttttca aaaactgaaa ttacacgatc tgatgtagtt gcctttagcc
     1141 cttgccattc agcaatctta ttacctaacc aagaagaagc aaagccagtt gtgtcatttt
     1201 tatattctat aaccgtcgca tcagttaatt tcttctcttt ttttagggaa tctaatgcat
     1261 cttcagagta accaatttga tccaccaacc cattttctac tgcttggtta ccatcataaa
     1321 ttcttccatc cgcaagttct ttcactgcat tagtatccat atttcttcct tttgctacaa
     1381 cattgacaaa acgatcataa gcactcatca caaattcctg tataacttta ttctcttcct
     1441 ctgaaggttt gtgttgaggt gacatcatat cttttaatgc accactttta tacgtcgcat
     1501 cagttacgcc aagtttttct aataagccag aataatttaa accagaaata atgacaccaa
     1561 ttgatccagt tgttgtttct tccgtagcaa atattttatc tgaaccagca gaaatgtagt
     1621 accctccact tgccgctgta tttttaaatg cagtatagat aggaatatct agctttttaa
     1681 tttttgccat ttctttagca atctcagcac tttcatatat tcctccccca ggtgaattta
     1741 cttctaataa cactccctta accgctttgt cttcttgaat tttctttagc tgtgttaaaa
     1801 aattctgatg gttatattgt tccctactaa aaagattgct ttcacctgta tctgcaataa
     1861 ctccattgac ggatagtttg acaatctttt tatttgatgc gccttcttct aacgtttctt
     1921 ctgtgatttc gttagatccg taaaaaatct tatttattcc tgttagggtt gtatttgatg
     1981 cttcctcttt atcttttcct ggaattagcg cgacgataat ggatagtaac aataatccgc
     2041 acgctactcc cacagcaatc catcttcttt tattcatata aactcctcct atattcttag
     2101 taaaaataat tattttttat ttagaaacac atcgacaatt tgttgccaat atgttttttc
     2161 tcctacaatt aaagacaact ctccagttaa tccataacga aacggaaacg attgagttgt
     2221 ttttaacgtt cctttcactg cataaaaaac acctttctca gtggcagtag aattttctga
     2281 aatttcatct aaagttccgt cgatcgtaat cggagcccca cctttttgat ccaatttaaa
     2341 atgaaccttc atcccttctt taatataggt actttcattc gcaggtaaaa gtgctgtaaa
     2401 tttcattttt gatttttcgt ggatcggata aatttctgca agcactgttc ctttggctag
     2461 ttctgcttgt ccctttgttt cttcatttaa atgaaccgtt ccagatacag gagcttttag
     2521 tattcctttt gaaagttgat cgtccaaact tttgataact gactggttct tctcttgttt
     2581 ttcatttagc tctgtaactg tttgttttac agttgttatt gcttgttcta gctcttgctt
     2641 tcctttttcc gtttgactaa ttctgtcatt ctcagaagta gttggtttaa ctaatttagc
     2701 tttttctgac tgatactgtt ctacttcttt ttttagttgc tcaatttgtt catttatgct
     2761 tgctgaaatt gtcagtttca cttgattttt ttgatcatca gaaacattat ttagttgttc
     2821 ttgccaattc tcatagtttg ccatcatttc ttttgaaaaa tcttttaatg attgattatt
     2881 actccaagct acctgtactt gttgccattc ttgtaattta ctatctgaac tctcaatttg
     2941 tttttctagt tgtctttttg tcttttcgta tacttctaat tgcttttgat catttaattc
     3001 actttgcttc aaagcgtagc ggagtgattc tttttctgac aacatgctct ttaattgatt
     3061 actgtagcca aaactgtcat ccgttgaaaa caaatttttt cctttactta tactttcaat
     3121 aaatagctga gccatcttta tttgttgagt aatacgttgg ttttcttgtt ctatctgagc
     3181 cttttcattc tctattaaag aacaatccaa tctaaccaac acatcgtcct tatgaacaaa
     3241 ttgattttct tttaggaagt tttccattat tttagcttct aatggcactt gtaacttctc
     3301 tattttttcg gggttcaatt gagccgttgt ccgaataaca acctcttttt tagcaaaggt
     3361 taaaaacaat ccaataatac ataaaaaaag aattacaggg tataacaccc aacggtagaa
     3421 gctatgatgt tgtttagtat agatactaga atgttcgaac caattattat tctgcataaa
     3481 ttgtttccct ctcctagatt tcccataatt tttgatagat accatcggca aaacgaagtt
     3541 catcgtgcgt tcctttttct accaaatgcc cttcatggag aactaaaatt tgatcacatg
     3601 cttttgctat tggtaaatga tgcgcaataa aaataatcgt tttttctttt aaattgagga
     3661 gataattcat aattgcatgt tctaacaagg tatctaatcc actagttgct tcatctaaaa
     3721 ttaatatgtc cggatttttt aataaagccc gtgctaaggc taatcgttgt cgttgccctc
     3781 ctgaaagatt atttccacct tcttccaaca atgtctcgta acgtaatggc tgttgattaa
     3841 taaattcatt tagttggact atctcacaaa cttctattat ttgttcgaaa cttggtgggc
     3901 tatctaaacc gaatataaga ttctcaatga ttgttccact aaagaaaaat gactcttgag
     3961 gcaaatatgt cactcgatcg cgtaatatct gaaaaggaat gtctaagtga ttaatgtttc
     4021 cataatggat cgatccttct gatgcttcat aaaaacgaac aaataacttt gcaagagttg
     4081 attttccaga accactaact ccaactatag ccgttttact gtgcgaagga attgtacatg
     4141 aaatttcgtt caacgttggg gccttcatgc tatacgagaa tgaaacgttt tttacagtga
     4201 tatcttgttg aaataattgt tcagaaagca tatattcaaa agactggttc gatttttctg
     4261 aatcaatcgc gaaaatttca tttaaacgat tatttgcaac ttgagccgtt tgcattttca
     4321 cttgtaaatt aataatgttt tgtaaaggat ctgtcaaaaa tactagtaaa gcattgtatg
     4381 tgataagttg acctagacta attctattat ccataacaaa ataagaacct agccataaaa
     4441 ttaatgcact gctaattaat tgaatcaaat gtttaattcc ttgttgaata ttatcaagcg
     4501 taaccgtacg aaatgatttt ttcattaatt taacaaattc atggtctata cggttatata
     4561 ctttctcttc accgctatat gctttaattg tttctattcc tttaagactt tctatgatac
     4621 tagaatttac agctgcaccg gctgccattt cttccctgtt cgatttttca tagcttttca
     4681 cgaaagcgaa tattgcaact acataaaaag gcaaagagca tagagtgatt aaaaacaacg
     4741 tactattctg aatagctaac gtaaccccaa caagcaacac cataccaata tctaaaaata
     4801 ttgataacgt agcacttgct aaagcatcaa taatcttact ggcatcaaga aaacgagaaa
     4861 taatttcccc tgattttctc gtagcaaaaa aattcattgg taaagttaac acatgtttga
     4921 aatactgaag cataattgcc atactcattc gctgtcctaa aataataaga agataactac
     4981 gactatattc aaacaatacc cgaaataaat agacagcgat caagccaatg gaaattatat
     5041 ttaaagttga acgtgcttga ttagggatga aaaaatcaag aataccttga aaataatatg
     5101 agcttcctat gccaaaaaac gtaatgaaaa tcgatgctaa aacgatgtgg aatactaatc
     5161 ctttttgttt ccaaattata ggcaaaaaag aacttaatcc agctactttt tctttaatag
     5221 gaatataagt ttctctaggc ataggtaata aaagaatacc tgtccattct ttttgaaacg
     5281 cttcaatcgt ttttctattt tttccattag cgggatctgc aatccatagt acgtttccct
     5341 tcgttttata aattacaacg tagtgcatat agctgttatc aaccaatata tgtgcaatta
     5401 atggtaagat aagatctctt tctttccaga tctcactatc tgcttgaata gccatacaat
     5461 caaaagacaa tttttcaaat gtctttttca atccaaatgc tgaagtaccc tctaaatcag
     5521 tcccagatag gtcccgtaat ttatgaatcg ggatatcagt tttaaaatat tttaaaatca
     5581 tcgaaaggca agcaacacca caatcttttt catcttgttg ctgcacaaaa ggaaatgatt
     5641 ttatcactta tctaacacaa ctccttctaa aaaattttta tagaaaaatt ctcaatatag
     5701 ctttaataca gtgaaaatat tgtctcataa ttccacaaaa actttgaaaa cagttcttga
     5761 acactaactt tttcattcct gaatcaaatt tattttggaa attttactat tcaagaataa
     5821 acttgataag tttcaggaag atttttactg ttaatcaaat acgtatgtta cagtagcacc
     5881 gtaaaaaata ttatttaaag aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat
     5941 tgtggaaatg cacaaactgt tggtggaggc aataatgctt ggggaaaatt ggggcaagtt
     6001 gtaggtggac ttaccacagg ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga
     6061 ccagcttgcg gtgttgttgg agggttatat ggagctgtcg ctggaggtgc tgcagctggc
     6121 tgggatgctc gaaaaaaata aaaaaggaga ataagtagta tgcatattaa aaacacaaaa
     6181 acaacattta tattatcttc agaagagttg aaaaatattc aaggtgggag tgccgtggga
     6241 gttttaggca ctactttttc tggggcaaca gcaggagtaa aactttgttc agccggagga
     6301 ccttatgcaa tagctgcttg tggtgtaggt ggagctttat taggtgctgg attcagcatg
     6361 tggaccggtg cgtaaacaat taaaaaatat caaacctcaa gattggtttg tgtcgattac
     6421 gaacataggt atgttcttta tcttttcaaa agtttggaat ggacagaact ttttactaaa
     6481 gcttcttgtg ctagttttta taattatatt aaatttttat cttttattaa agtcagataa
     6541 aaattctttg gaaatgtaat ggtagagata tttttacaaa aggacttgca gcgctgtagt
     6601 tggtgctgca ggaggaccta tttcggccgt tacaggaggt attggtaatg tacttgtcgg
     6661 atgtgcatta aagcctgttc ctgcaagtta aatttataaa aacaggaaag taattatctc
     6721 aaatcctatc aagtacaatc ataggattgc acttgacagg acaaattttt taactaaaaa
     6781 atgaaaaaca ctttctaaaa attagtagaa tttgccaaat ctttcgcatc ttccaccata
     6841 catttaattt taattcccct ttatggtact taataaaaac aagaaaagaa ctattcattt
     6901 ggcccttttt tggttccaga ccatagaaat attcctccag taactaatat tgctgttatg
     6961 cacgtaacta ataacagcta taaaaattgg tacaacttta ataggattca gaaaaatttg
     7021 cactagtgaa ggaatctatt ttattagaaa aatgcaatct ccctaaagaa aaaaccacta
     7081 aacagtgctt tctttcaact tctccttaat tttttgtgct ttccctaaag taaagtttct
     7141 ggccaactct gaatcaaaat atagtgaacg ttttttaaaa tctatttctt taacgttaaa
     7201 gatatttact aaacacgctc gactaattct tgttaacata ggatatttct tttcaagctc
     7261 actaattctt ccataaaatt cgtactgccc atctttagta cacaatgata atctatgtgg
     7321 taattttgaa gcttctacaa aataaatgtc atttatatca aaagtaaatg tttgagaacc
     7381 tatggaaaaa acaaatgctt gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc
     7441 taataacaat aactcaacaa tctcgtttcg atactcatct agtgtttgat cttttgttac
     7501 aaatcctaac gtttcaactc ttctttttat tgttacgggt aacatctcat catgagtagt
     7561 tgtaaaaatg atttttgctt gaacatcata ttttctgatt acttctgcta attctatacc
     7621 gttaacttca tgatttaaat caatatctaa gaaatatatt ccttgctttg gtcggaattg
     7681 tttgaggtat tttttaactt ctaacggact ttgtgttttc aatactatct taaatacttt
     7741 atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt gtataatttg
     7801 atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa caacaaaata
     7861 acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt gggattcttt
     7921 ttattgattt cctctatact agataaacct aacccttgat gtcccttttt tgttgttatt
     7981 ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt cgatatcgaa
     8041 atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc ttgactctct
     8101 atagatgctt caattgcatt atcgagaata attccaagca ccctaataca gtcaaatatt
     8161 ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat cttattgtta
     8221 tttgcttggt atagcttcgc tatcaacaaa cttttcaaat attcattttt tatatttttt
     8281 aaataacgat attcaaactt attggttaga taagaattag aatagatctc taattctttt
     8341 atttcttttt ccaatatcga atcatggttt tgattagcga tttcttttaa actcagcaat
     8401 aaatttttat agtcatgtct aaatctggca aatttttctt gatctttttc cagtgaatca
     8461 gtatattttt ttagataaac caattctttt cttttcaatt gctgttcata ttgttctcgt
     8521 tgcctaatag ttattctaat aaataaaaat agaacaacaa acatttgaat gattaaaaat
     8581 attaagacac caaacacaaa atgatcaaat acttgatatt gatgagcaac ataagaaaca
     8641 aataaaccaa ttgtaagtaa ataaatcatg aatatagcag taagtttaga actggactgt
     8701 tcaacaatcc tatttatagc taattttcta tataaccaaa taaacatcag caaaaacaaa
     8761 gaatcaagca ttatctggaa tactacataa ccataccctt ttatcccttc aaatgaaaac
     8821 atatacgtca taaccatgga agtaataata gatctgaaat catcaataag catacataat
     8881 agtaatttat ttaaaagcac tatattagtt ttcttctttt cctttaatat aaaataactt
     8941 ccgattatag gtatagctgc ggaaacatca gtaaataatt caacaaaaaa agtgagtggt
     9001 atcaataaac atacaaaaag atattttctt ttgttaagat cagtattagt catataaaat
     9061 aagagaaaaa agaaaaagtt tcccacgaac attcttaata gataagcttt tgtatcgata
     9121 gtaatcaaga gatcccctcc tgattttagg atatcataaa aaaaatttta gttacacttt
     9181 ttaaataatg aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt
     9241 ccagcacctc ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta
     9301 tttttttctt ccattacgat tccccctaca aactataaat acttttat
//
LOCUS       DACCWV010000108         9266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCWV010000108 DACCWV010000000
VERSION     DACCWV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.110"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            296..829
                     /locus_tag="IVW84_002198"
     CDS             296..829
                     /locus_tag="IVW84_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918379.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(955..1632)
                     /gene="nth"
                     /locus_tag="IVW84_002199"
     CDS             complement(955..1632)
                     /gene="nth"
                     /locus_tag="IVW84_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7918380.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1639..2337)
                     /locus_tag="IVW84_002200"
     CDS             complement(1639..2337)
                     /locus_tag="IVW84_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7918381.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2605..3789
                     /locus_tag="IVW84_002201"
     CDS             2605..3789
                     /locus_tag="IVW84_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7918382.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3795..4238)
                     /locus_tag="IVW84_002202"
     CDS             complement(3795..4238)
                     /locus_tag="IVW84_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7918383.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4464..4751)
                     /locus_tag="IVW84_002203"
     CDS             complement(4464..4751)
                     /locus_tag="IVW84_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918384.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4922..6274)
                     /locus_tag="IVW84_002204"
     CDS             complement(4922..6274)
                     /locus_tag="IVW84_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7918385.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6547..6993)
                     /locus_tag="IVW84_002205"
     CDS             complement(6547..6993)
                     /locus_tag="IVW84_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7918386.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="IVW84_002206"
     CDS             complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="IVW84_002206"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7918387.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(8295..9182)
                     /locus_tag="IVW84_002207"
     CDS             complement(8295..9182)
                     /locus_tag="IVW84_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7918388.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2563 a         1863 c         1499 g         3341 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat
      361 cataatttgt ctgcaccatc tttttttatc gaaaacaata cctcgttatt ccaagaataa
      421 aaaagctgaa aaattttgtt tattattaaa taaatttact ttaattgctc ctatccttgc
      481 atttattatt ttttctgtac ttttaagcac tactcttaaa ggaaaattta tggaaagaag
      541 ctctcatgca atgattctca cttttttatg gttgttgttc accagaatct atatttttct
      601 gatgagcctg aaaccgccca aatccattag tttatgttta gttataaatg gtattttttt
      661 attatcactt atcattttta ttacaccttt agatagatac gtcacttatt tatataatcc
      721 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
      781 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      841 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      901 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      961 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
     1021 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
     1081 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
     1141 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
     1201 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
     1261 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
     1321 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
     1381 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
     1441 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
     1501 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
     1561 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
     1621 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
     1681 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1741 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1801 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1861 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1921 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1981 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     2041 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     2101 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     2161 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     2221 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     2281 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     2341 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     2401 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     2461 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     2521 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     2581 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     2641 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     2701 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2761 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2821 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2881 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2941 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     3001 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     3061 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     3121 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     3181 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     3241 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     3301 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     3361 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     3421 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     3481 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     3541 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     3601 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     3661 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3721 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3781 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3841 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3901 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3961 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     4021 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     4081 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     4141 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     4201 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     4261 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     4321 atctgaaacc aagaaaaaaa ggggttgaca aagcttgtca taaccccttt ttctgattaa
     4381 aagatagtaa agagacagtc atcggtaatt gtgatgattt tttgtcatca cttaaggatg
     4441 agcggccttt tcaaaaactg tctttaaaga tcatcatcag tttctagcac atatggctga
     4501 ccttcatatg tgtactctgt attcaatgtg ccgtcatttt catctgagta gatgcgtcta
     4561 ctgaaaggaa aacgatgaga aacattatct ttggcgtcat ttgctggcat cgtgtaatat
     4621 tcctcttgcg tttcattcaa ctgagagagt acttgatctg ccaactcatc aatattgatc
     4681 gttaatttct tcgaagcact tttataatct tctaaaaatg catcttgttg gacaaaatat
     4741 tctttattca tcttctttct cacctttctt ttcaaaatca aataaagctg cttgttcttt
     4801 tatgtaacag ctttatcttt gctgcttatt gtgacaaaaa attcgaagaa agacaactaa
     4861 ttgtactttt cacaaaaaaa gacagagggt cgtgacaaaa ttgtgtcaca accctttttc
     4921 tttattgtgc agtttcgatt tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc
     4981 atgggcggtc aaaatgtggt tggaagaaga aatctgaaag tgcgagatct tctacagtca
     5041 ttttattttg aacagctaaa gacatggtat ttgctgattg tgtaatgtcg tatttggaca
     5101 tgaactgagc acctacgata cgattcgttc ctttttcata gactagctcc atcattactt
     5161 tttcagtagt tggcatgaac tctggacgat aattatcttc aaatacagtc gcttggactt
     5221 ctagatcgtt caaagttgcg ctttctttgg tcacacctgt cgaaccgatc gtccagccaa
     5281 ataggtaaag tcctgaagtt ccttgagtac cacgatacgc cattttatga tctgtcaagt
     5341 tatttcccac aagcaaacct tgacgaacgg catttgttgc caaaggaata tagttttgcg
     5401 tgccacttgg attgtagttg actactgcag agtctccagc tgcaaaaatg tctggattac
     5461 ttgtctgcat gtattcattc actttgattg ccccgtttgg cagtgtatct acttttcctt
     5521 cgatcagttt tgtatttgga cggaagccca cacataaaat gaccatatct gtatcaaatg
     5581 tatctgttgg tgtagctact tgtttcactt ttccttcttc atctgtgata aattcagtaa
     5641 cattttcgcc tagcgcaaga gtaacaccgc ggtctgtcaa ttctttttct aagatatctg
     5701 tgaatggttt atctagatat ttattcaaga tgcgatctaa tccatcaatc aatgtcactt
     5761 gttttccaga ttcgacaaaa gcttctacta attcaatacc gatatatcca ccaccaacaa
     5821 ttactacacg ttttgcttct ttcgcttgtg caataatttc atttgcttga ttgtagtttt
     5881 tgcataggag tacatttttc gcatcgattc cactaatcgg tgggataata ggccatgaac
     5941 cagttgtcat cactaattta tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt
     6001 ttgccgttac tgttttattt tctgtatcga cgtctgttac atcgtgttcc attttaactg
     6061 ttgctcctaa tgaagataat tcttcaggat ttgagtagaa caagcctgct gggtccttta
     6121 ctactccgcc gacataaagt gcgataccgc aagataagaa agaaatattg tcatttcttt
     6181 catatactgt cacttctgct tctggatgat tttttaaaat actttttaca gctgcagtac
     6241 ctgcatgagt acagccaatt actactactt tcatttatgg tccctccgat taacattttg
     6301 tccttcgaca aaactactat aacagaaata aaacggatac aacagtttaa tgcttgtgaa
     6361 ttcacaaagg tttgtttgta gtttcattcc ttttcaagca taagaaccca atatctacaa
     6421 gattcttatt ttaataatat tcacttttat atctaagatt gtgaataaaa gacaaaaaaa
     6481 taaaaaagct agcgtttttt ttacacagct agcttttttt aggtataaca cttgtagaaa
     6541 ctgtttttac atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa
     6601 ttccaaccga tgttcattaa tctcaaaatc agtcagtctg cttgcagcca tttctatatc
     6661 ttcaagccct gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata
     6721 atgacgcttt gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac
     6781 aaatccaatc gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg
     6841 aaaccgatgt tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat
     6901 caaatattct aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt
     6961 ttcgataacc tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt
     7021 cttatcatac tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta
     7081 ttgtatttta atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca
     7141 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
     7201 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
     7261 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
     7321 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
     7381 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
     7441 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
     7501 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
     7561 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
     7621 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
     7681 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
     7741 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
     7801 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
     7861 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
     7921 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
     7981 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
     8041 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
     8101 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     8161 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     8221 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aatttctttg
     8281 cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata actggacata
     8341 gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg tgcaatatcc
     8401 catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt tacatctgtt
     8461 gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact tgtggttgtt
     8521 gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt aatcaatgag
     8581 ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta cgctgttgca
     8641 tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc gcccgtggaa
     8701 aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata agaagaatat
     8761 tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt ggttgccata
     8821 taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt tggcgcacta
     8881 gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat gactgaagca
     8941 tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc gataaacgct
     9001 tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg cggtaccgct
     9061 gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat cgctttttta
     9121 ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt cagctcttgc
     9181 attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt aaaacaaact
     9241 taattaatta aattatactt ttaatt
//
LOCUS       DACCWV010000109         9098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCWV010000109 DACCWV010000000
VERSION     DACCWV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.111"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..776
                     /locus_tag="IVW84_002208"
     CDS             <1..776
                     /locus_tag="IVW84_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918389.1"
                     /translation="YFRLSFMKTMIASFCLSLFFELTQLSGLYFIYPRPYRLFDVNDL
                     VTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRLVAYLIDWTFLSLIN
                     LVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLGKKIVRIRLVEEDRE
                     RISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSAVFISLGVLLLQGIF
                     ILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            846..2195
                     /locus_tag="IVW84_002209"
     CDS             846..2195
                     /locus_tag="IVW84_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7918390.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(2240..2671)
                     /locus_tag="IVW84_002210"
     CDS             complement(2240..2671)
                     /locus_tag="IVW84_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918391.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            3059..5701
                     /gene="alaS"
                     /locus_tag="IVW84_002211"
     CDS             3059..5701
                     /gene="alaS"
                     /locus_tag="IVW84_002211"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7918392.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5901..6497
                     /locus_tag="IVW84_002212"
     CDS             5901..6497
                     /locus_tag="IVW84_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7918393.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            6619..7767
                     /locus_tag="IVW84_002213"
     CDS             6619..7767
                     /locus_tag="IVW84_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7918394.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(7805..8218)
                     /locus_tag="IVW84_002214"
     CDS             complement(7805..8218)
                     /locus_tag="IVW84_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7918395.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            8297..8728
                     /locus_tag="IVW84_002215"
     CDS             8297..8728
                     /locus_tag="IVW84_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7918396.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(8827..9060)
                     /locus_tag="IVW84_002216"
     CDS             complement(8827..9060)
                     /locus_tag="IVW84_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7918397.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         2988 a         1595 c         1852 g         2663 t
ORIGIN      
        1 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
       61 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
      121 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
      181 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
      241 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
      301 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
      361 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
      421 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
      481 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
      541 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
      601 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
      661 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
      721 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
      781 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
      841 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
      901 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
      961 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
     1021 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
     1081 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
     1141 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
     1201 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
     1261 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
     1321 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
     1381 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
     1441 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
     1501 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
     1561 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
     1621 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
     1681 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
     1741 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
     1801 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
     1861 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
     1921 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
     1981 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
     2041 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
     2101 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
     2161 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
     2221 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
     2281 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
     2341 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
     2401 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
     2461 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
     2521 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
     2581 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
     2641 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
     2701 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
     2761 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga
     2821 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
     2881 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
     2941 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
     3001 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
     3061 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg
     3121 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
     3181 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
     3241 aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
     3301 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
     3361 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
     3421 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
     3481 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
     3541 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
     3601 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
     3661 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
     3721 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
     3781 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
     3841 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
     3901 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
     3961 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
     4021 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
     4081 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
     4141 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
     4201 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
     4261 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
     4321 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
     4381 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
     4441 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
     4501 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta
     4561 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
     4621 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
     4681 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
     4741 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
     4801 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
     4861 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
     4921 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
     4981 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
     5041 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
     5101 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
     5161 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
     5221 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
     5281 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
     5341 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
     5401 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
     5461 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
     5521 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
     5581 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
     5641 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
     5701 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
     5761 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
     5821 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
     5881 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
     5941 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
     6001 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
     6061 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
     6121 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
     6181 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
     6241 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
     6301 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
     6361 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
     6421 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
     6481 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
     6541 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
     6601 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
     6661 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
     6721 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
     6781 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
     6841 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
     6901 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
     6961 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
     7021 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
     7081 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
     7141 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
     7201 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
     7261 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
     7321 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
     7381 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
     7441 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
     7501 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
     7561 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
     7621 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
     7681 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
     7741 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
     7801 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
     7861 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
     7921 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
     7981 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
     8041 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
     8101 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
     8161 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
     8221 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
     8281 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
     8341 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
     8401 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
     8461 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
     8521 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
     8581 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
     8641 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
     8701 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
     8761 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
     8821 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
     8881 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
     8941 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
     9001 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
     9061 agcactcacg acctttctaa agtctgatca tagccttc
//
LOCUS       DACCWV010000110         9067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCWV010000110 DACCWV010000000
VERSION     DACCWV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.112"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..305
                     /locus_tag="IVW84_002217"
     CDS             6..305
                     /locus_tag="IVW84_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918398.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(402..836)
                     /locus_tag="IVW84_002218"
     CDS             complement(402..836)
                     /locus_tag="IVW84_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918399.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1068..1778)
                     /locus_tag="IVW84_002219"
     CDS             complement(1068..1778)
                     /locus_tag="IVW84_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7918400.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1768..2628)
                     /locus_tag="IVW84_002220"
     CDS             complement(1768..2628)
                     /locus_tag="IVW84_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7918401.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2633..3271)
                     /locus_tag="IVW84_002221"
     CDS             complement(2633..3271)
                     /locus_tag="IVW84_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7918402.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3286..3585)
                     /locus_tag="IVW84_002222"
     CDS             complement(3286..3585)
                     /locus_tag="IVW84_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918403.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3628..5112)
                     /locus_tag="IVW84_002223"
     CDS             complement(3628..5112)
                     /locus_tag="IVW84_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7918404.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5132..5593)
                     /locus_tag="IVW84_002224"
     CDS             complement(5132..5593)
                     /locus_tag="IVW84_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7918405.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="IVW84_002225"
     CDS             complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="IVW84_002225"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7918406.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6896..7666
                     /locus_tag="IVW84_002226"
     CDS             6896..7666
                     /locus_tag="IVW84_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7918407.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7939..8766)
                     /locus_tag="IVW84_002227"
     CDS             complement(7939..8766)
                     /locus_tag="IVW84_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918408.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2768 a         1893 c         1412 g         2994 t
ORIGIN      
        1 aaaacatgga ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag
       61 aacatttcat gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt
      121 tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca
      181 aaattaataa aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt
      241 tttatattag ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa
      301 aatagctgag cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa
      361 agaactaagc tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa
      421 agcacctttt ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga
      481 acaaatccta ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg
      541 atttggactg gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct
      601 attccttggc gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca
      661 aaatcagtga tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga
      721 gattcagatt gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa
      781 ccaacgctct tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt
      841 ttcttccttt cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat
      901 cacatataac tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga
      961 atgcatactt tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct
     1021 ctctatcaag tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag
     1081 tatgcatact ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca
     1141 catatattgg ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc
     1201 aaactgtttt ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag
     1261 acaagtactc cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc
     1321 acattccctg tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta
     1381 caaggaacct taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt
     1441 cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct
     1501 tcaaaagttt gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct
     1561 tcttctatta tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca
     1621 tatctgacac cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca
     1681 ttgccccaag tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca
     1741 cgttgcttca ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt
     1801 agaacttaag tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact
     1861 ccacatttca atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc
     1921 aagaaaatca acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt
     1981 tgttacaggg taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag
     2041 ttctcgagcc ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg
     2101 tagaaaaggt gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa
     2161 tggatcatcc atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct
     2221 ttcttttatt ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac
     2281 atcgtatcca gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt
     2341 tttcattagt tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg
     2401 ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct
     2461 cagctcagct atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc
     2521 aatcgacatc tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca
     2581 tgaaatatct ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc
     2641 agatacgaat gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg
     2701 taataccacg accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt
     2761 gtggatttaa tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct
     2821 tcgataaatc tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt
     2881 agattacctg ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc
     2941 cgtagagttc tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg
     3001 ttgcacaaca aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg
     3061 ttcctccagc gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc
     3121 gaatacagcg aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa
     3181 tatctactac atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat
     3241 ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg
     3301 gtaccacttt gtttcagcaa atttttcact gatttctttg actgacatca catttttgat
     3361 accgataact gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg
     3421 cgtaaaaact acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg
     3481 gattttggta attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat
     3541 caagcttgtt cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt
     3601 atgagtgttg tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata
     3661 gtcttctaca atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc
     3721 caataaacca atgacaacta cggcaattcc agcataacct aggactttca tgattactgt
     3781 aaacaatggc caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat
     3841 cccaatccat gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg
     3901 gaacaggcaa gcggctttga atccacctcg gttattggca taaactgcta taacggcatt
     3961 atcaaagaac aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag
     4021 taaaccaatc atcaaaaact gtcccaaagc accaaacaag aacccaatcg ttaccgcatt
     4081 tggcgaacca aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat
     4141 cgtgtttgag attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa
     4201 ttgcaagatc gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa
     4261 actctgccct tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag
     4321 aatgatccca aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat
     4381 cgacaagaat cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt
     4441 tttcttttca tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa
     4501 catttgctga tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac
     4561 tgttaagtta gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc
     4621 ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg
     4681 ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg
     4741 gactaataag atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt
     4801 acggccaaat gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt
     4861 aaccatggca tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt
     4921 gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc
     4981 agcgatgcat tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc
     5041 aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa
     5101 aaattccccc atcttttttc tcctctatca attaattata ttctgccatc acaacttggt
     5161 aatcatcgat ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt
     5221 cggataattt ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa
     5281 acaaactagc cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca
     5341 acttggtaaa tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca
     5401 ttgctacttg aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct
     5461 cctccacata cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga
     5521 tagcttcacg ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa
     5581 agtatttcag caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt
     5641 ttcaatcgag aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt
     5701 tgtcggatag atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag
     5761 gcgatccttt ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga
     5821 ccaaatatcg tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag
     5881 catatcaaca gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa
     5941 acagacatcg attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc
     6001 tcctgcatga taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc
     6061 catctcctga ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc
     6121 tgtccgatca aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc
     6181 tggctttact gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac
     6241 ttcttttggc ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg
     6301 gatatctaga tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc
     6361 aaaaggatcg attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact
     6421 catacgcatc atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt
     6481 cccacaccat agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt
     6541 acagcccagc caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc
     6601 attcaagtat gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc
     6661 attgacagtt ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa
     6721 tattaatgtg tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga
     6781 ttttctttca gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat
     6841 gaacaactag tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa
     6901 aaactcactt gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac
     6961 tgaacaacta tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg
     7021 aagagattta aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc
     7081 ttataattac aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc
     7141 gattgccaga gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag
     7201 ctcgactgcg ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa
     7261 caacgtgaaa atcaattact ctccgcacaa agataattgt agttatattt taactggtgg
     7321 agagatgcgc tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc
     7381 agtcaatgca gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac
     7441 aacaaagata ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg
     7501 taagatttta gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga
     7561 tatttcagtc tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga
     7621 gatcagaaat acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa
     7681 atatgtaaat aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg
     7741 ctacgctcaa attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat
     7801 ctattgcttc ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa
     7861 aaggaggtat gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac
     7921 actgatttca caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt
     7981 tccgttgaaa cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc
     8041 taattgctta tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt
     8101 atgtttctta ttatactcaa tacccttctc acataattgg attgctttat cataatcctc
     8161 gattttacgg taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa
     8221 atcagtctca ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc
     8281 tccttttgac aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt
     8341 ttcggtcgtt tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc
     8401 ataaagatga tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact
     8461 tactttttcc tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga
     8521 attcaatgat tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa
     8581 tatatcggag atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg
     8641 cgacatattc ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa
     8701 ttgtacttgt gttaacccct ttttctttcg gtattctttt agtaatgctc catctacttt
     8761 aatcaaaaca atttccttcc ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa
     8821 aagttctaaa caaccaaaaa tgtttagaac agaataaaaa ttttaatttt aaaataatat
     8881 tcaaattaaa caactggcgt atctgctcct tgatttcttt ttgtgttatc tgaataatta
     8941 tcgtttacag ttataacaca tagtgaaaat tgtcatggcg agaaagtcta agaatatttt
     9001 taaacataac tatttctttc ttacgtgtgt ataatgacga cagcacacct atcaattaat
     9061 ataatta
//
LOCUS       DACCWV010000111         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCWV010000111 DACCWV010000000
VERSION     DACCWV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.113"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            51..260
                     /locus_tag="IVW84_002228"
     CDS             51..260
                     /locus_tag="IVW84_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918409.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IVW84_002229"
                     /pseudo
     CDS             467..667
                     /locus_tag="IVW84_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IVW84_002230"
     CDS             complement(1379..1984)
                     /locus_tag="IVW84_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7918410.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IVW84_002231"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IVW84_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IVW84_002232"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IVW84_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IVW84_002233"
     CDS             3019..3990
                     /locus_tag="IVW84_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7918411.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IVW84_002234"
     CDS             4130..5443
                     /locus_tag="IVW84_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7918412.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IVW84_002235"
     CDS             5486..6352
                     /locus_tag="IVW84_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7918413.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IVW84_002236"
     CDS             6349..7197
                     /locus_tag="IVW84_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7918414.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IVW84_002237"
     CDS             7211..8683
                     /locus_tag="IVW84_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7918415.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="IVW84_002238"
     CDS             8700..>8942
                     /locus_tag="IVW84_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7918416.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DACCWV010000112         8900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCWV010000112 DACCWV010000000
VERSION     DACCWV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.114"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1152..1550)
                     /locus_tag="IVW84_002239"
     CDS             complement(1152..1550)
                     /locus_tag="IVW84_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918417.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1766..2821
                     /locus_tag="IVW84_002240"
     CDS             1766..2821
                     /locus_tag="IVW84_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7918418.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2841..3194
                     /locus_tag="IVW84_002241"
     CDS             2841..3194
                     /locus_tag="IVW84_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918419.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3376..3672)
                     /locus_tag="IVW84_002242"
     CDS             complement(3376..3672)
                     /locus_tag="IVW84_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918420.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3676..4494)
                     /locus_tag="IVW84_002243"
     CDS             complement(3676..4494)
                     /locus_tag="IVW84_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7918421.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4720..4941
                     /locus_tag="IVW84_002244"
     CDS             4720..4941
                     /locus_tag="IVW84_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP7918422.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4943..5209
                     /locus_tag="IVW84_002245"
     CDS             4943..5209
                     /locus_tag="IVW84_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7918423.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5322..5861
                     /locus_tag="IVW84_002246"
     CDS             5322..5861
                     /locus_tag="IVW84_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918424.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6355..6639
                     /locus_tag="IVW84_002247"
     CDS             6355..6639
                     /locus_tag="IVW84_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918425.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6913..7167
                     /locus_tag="IVW84_002248"
     CDS             6913..7167
                     /locus_tag="IVW84_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918426.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7249..7434
                     /locus_tag="IVW84_002249"
     CDS             7249..7434
                     /locus_tag="IVW84_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP7918427.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7501..7896
                     /locus_tag="IVW84_002250"
     CDS             7501..7896
                     /locus_tag="IVW84_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7918428.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            7886..8263
                     /locus_tag="IVW84_002251"
     CDS             7886..8263
                     /locus_tag="IVW84_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918429.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8264..8659
                     /locus_tag="IVW84_002252"
     CDS             8264..8659
                     /locus_tag="IVW84_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918430.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         3121 a         1495 c         1391 g         2893 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc attttttgga tgagattaat
      421 atcaataaat aacgatgtaa aaatcattta ttatgctctt ttataaggat attaattgat
      481 ctaatttgaa aaaaaatcaa aatttaaatg ttttaatatt acaaataaaa tcatgttatt
      541 gtaaaatggt ataaaaaagc taaatatttt atttttgtta ttttattagc aggaaaattt
      601 aaatagtttt acaagaatcc gtagaaatat attggttttg ccttcagatt aatactggat
      661 catattaatc tgaatataat atatgaacta ataaatagct taacctcgct attttgtttt
      721 ttctgatcac tatctataca gcattagtaa atagaatttt aaaaataaaa aaattaagac
      781 aaaaaagtat caacataaac atatacatat acacgtttat gttgattcac ttaatttgtc
      841 ctttaaaaag cacttatttt aatataatgt tattttataa tcttattaca agctgattgt
      901 taggcgttgt cctttcatta catttattaa cggttatttt attgtttaat ttattaatta
      961 tttttgatct aaaacttagc ttaacaaata aataattaac caaccgccag ataaaaatgc
     1021 gatcattgct aatacataag caaagataaa aatatgttcg atcagcttaa accctgatgc
     1081 gattaagcag accaagtaaa atagaaattc tcttacagat aatggtaatt ttctaactag
     1141 catactcata attaacaccc caaagtaata ttgtattttg aacattttac atccaataca
     1201 gcatcaatca accattgcca atcaaaaata caaggcttca ttgaagtatg tttttcagat
     1261 gaaaagatat aatataattt cttcggaata atattttcag ttgttgggat caagctaaat
     1321 ctaataccgt ctaactcata gctaccagct ttctctccat taaaaatttc cttagcactt
     1381 tcttctaaag aatcaaaata tttttcgaca gcaatctttg aagcttttac tattgaagca
     1441 tttccctttt cgataaaatc tgcttttgtt ttaccgacaa taagtaattc ttgatcagta
     1501 aaatcttcta aaataatggt agtgtaattc ttatttcttc tcaatcgcat caggatcact
     1561 ccttaatata atttatgttt ttataatatc ataataagat aaaattgcaa gcatttattt
     1621 acaccttttt atttacactt tattataatt aattagactt ttatgattgc atttttttct
     1681 tataaacagt tgcaactaat agtaaatagg tgtaaactat tttcgtaccg aaataccata
     1741 aacacaaaca gaaagaggtc ataccatgaa agaaccatta ttgttttccg ttgacttagg
     1801 gaataaatca gtaaaagaaa tgagtacaca cgtttctcca ctctcacttc cctctcgtat
     1861 tttgaacaaa aatatcatta attcaggacg tttttctaat ataggttcct cattaaaacg
     1921 atcactaaac gtttctgaat atagcctagt aaacactact gaaagctatt actttgggaa
     1981 ggatatacac gagctaggaa aagatgacta cgtgatagaa tcactaggtt ttgggatcag
     2041 cagatacaag caggaaacct accaaaatat cctatctttc tctttagcta acctagcacg
     2101 tttagaagat caatctgatg acgttttaac cgctaatcta gttttaggtc tgccttcatc
     2161 cgactatacc gaaacaatcg tcaaatacgt gatgcaacaa gccacacgac aacattcagt
     2221 tgaagttgac ggtaaagctt ataatataaa cgtagatacc gtttgggtac tgccacagcc
     2281 gattggaaca ctatataacc tcctacttgc tgatgatggt agtgtacaag atgaaacatt
     2341 gatcagtcag aagatcggtt ttgttgatat tggcggtggc acgaaacttt ttgatgttgt
     2401 ccaaaatttt gaaattgacg aaaacaatcg tgaacaaaag gataccggca tttttacttt
     2461 atataatcgt atcgtaaata tgcttgacct tccgatcagt cctaatgctt tccaacttga
     2521 aaagattatc cgagaaggca aagaaaaagg cgtatatcta tttaagccaa gcggaaatga
     2581 aagttttgat atttcagaag tagttgaaag agaaataaaa acatacacca atatggttgt
     2641 tgcagaagtt ttagctacat tcaaaaactt cacaacctta gataaagttg tctttactgg
     2701 tggtggagct tctattatcg acaaacaact tgtaaaagat aaaattccgc ataccttgtt
     2761 tgttgaagac agtgaatttt ctaatgtaaa aggcttttac aagtttggtt taactcaata
     2821 aaacagcgaa ggagatcaac atgaataagg gaaaatcaga gcgttatgta gttataatca
     2881 atagagaaaa ggttagcgag ttacctttag cagagtttct tgaatccgaa tatgaaacac
     2941 taggcgttaa atactctcag acgatcaaaa aagctttgaa cctatatcgg cgtgtaaaga
     3001 acaatgaagt tacaattatt gaagcaggca acgatagaaa cgatagcaaa gtagcagaag
     3061 taaagaataa accaactgat cataacccta ttcaacaaga gcatccttca acggttacat
     3121 ccgatcattc agcaaagaaa gaaaagccaa tgctgaaagg gttcaatgca ccaagtaaca
     3181 aagacttact ttaaaaataa aaaggcgagg ttacaatacc tagccttttt attatgtaca
     3241 aatataaaca taaacatata cgtatacatg tatataaaca tgtatacgta tatatccata
     3301 aacacgtata ttaaatttag ttaagctata cctaaccgct tattccaata aaaaaagtga
     3361 tcagctttaa actgatcact caccaaaaac ttcatcatac aagttatcta tcatttcacc
     3421 aagtaaagct gaatctgatc gaattcctaa cttttcgctg attttagata gtttctttct
     3481 ttgcgatggt tgaagcgtga attgataatt tttcttcttt tcagaactat cactttttag
     3541 tttagctacc aaagaacttg ttgtttcttt aggtgtttgt ttaggttctt caactgcttc
     3601 aaccacttca ggaacttctt ctacttcagc ttcagcgaat tttttaaatc tattttcttt
     3661 cacttcaatc actccttatt cttgtgccgt atcaattatt ttgttgtata gcgtaaacgt
     3721 gctttcaaag aaatccttat tcttacgata gatattctta tccttagcga actcagcaat
     3781 attcttacct tctaagctag ttgcattaag caattccttc tcttgtacat atccaagata
     3841 attagataac gttggtacca gttctaggaa ctcacgactt gaattagtat tatgtttgat
     3901 cctgctaccg atcgtagcgt atttagtttc catcagtgtt tcaccagtct taatatcaat
     3961 caactgtttt gctagtgcat caatatcttt accaagcttc agcaaagctt caaaaccatc
     4021 tttagatgga tcagaaatgc ctacgacaag atcagataca gcccaagcat tttgagctag
     4081 tagacctgca tcattatgag tatcaatcag aattagatca tacttctctt taagctgttc
     4141 atagttaaca accatccatc taaacagtag ggttctgttg ttaggcatat cacgcatttg
     4201 catctcaact tgttttaaat cttcacctgc tactaaaata tccatgttct catttacagc
     4261 taaaggttca gctgatccat gaagatataa atgattgatt gtattctctc ttttcgcttt
     4321 actgaaagca tcaggaaatc gttttgatag gttttgcgac ttatcttcat caatatacag
     4381 caccttcata cctaaatttt cgacagcata tccacctaga ttgtagattg ttgttgtttt
     4441 acctgttcca cctttttcaa tgtttccagt aatgattgac aacttctttt ccatgattaa
     4501 acctccctta ttaacctttt gtaactccat tatacactat aaacacgttt atgtatatgt
     4561 ttatatacat aaacgtgttt atatttatat ttgcttttat attctacaac ataaacatat
     4621 atatgtatat gtttatgttt atgtatatat tcatatatat aactattgca tttgtcatac
     4681 aatcgtagta caatgtaatt gaaaaggagt tgataaacta tgccagtaat aagtattcgt
     4741 gtatctaaag aagaaaaaga ttttcttgaa aaagttgctg aattcaacgg agataacatt
     4801 tctgaattta tgcgtagtaa ttccctgcaa acagcagaaa cgttagtaga ctttgccacc
     4861 tacaaggaac taatgaaaga gcatgaggaa aacgatcaaa gtatttccca cgaagatatg
     4921 ctaaaagagt taggcttata agatgactta ccaagtaaga tatgaacgtg aagctcaaaa
     4981 agcgctaaaa aaaatggata agtttcaggc aaaggttatc ctaaactgga tcgagaaaaa
     5041 tcttgtaggc actgatgatc caagaaaaca tggtaaaggt ctaacagcta ataaatcagg
     5101 ctactggcga tatagggtag gtgcctaccg tataatagct gatattagcg agcatgaagt
     5161 gacaattctt atattgtcta ttggtcacag aagtgaagtt tataagtaac agacaagctg
     5221 ataaggcttg tctgtttttt tgttaaccca acaaaatcac tgtttacgat cccactttac
     5281 ctaaaaaaga acaataccct cttgttatta tacacactgg tgtgtataat ataagtgtaa
     5341 acaagctaat taactttcaa aaggagcgat tttttatggg atggtatggt gcttattcat
     5401 cagcaaatga agtgatcgaa gaaaagaaag ggtacgccaa aaatggtttg gaaatcgtag
     5461 ataccaaagt aacgaagacc tatggtatta tccttttcaa agatgcagaa ggaacctacg
     5521 atattgacag cttcatcttt cgagatgaaa tgtacaaacc aatctacttc ttaaatgaca
     5581 gtcctgctta ctttaagaaa attccgaaaa aatggttgaa attctttgtc ggtgatcaag
     5641 aaaaaatggt taagcaacaa ctagatagaa ttaaagaaga aaacaataaa ccaaaccttg
     5701 atgacttatt agttgcaggt aaaacttact caatctggga tggatcgtat gtcgctgttt
     5761 atcagtataa actcaaacgt agctttattt ttagactaca agatggatca ctcactcggt
     5821 tcactcgact atcagcagga gatgttaagg aagtagccta aaacttaaat acataaacat
     5881 aaacgtgtat acgtttatgt ttatgtttat gtatatgtat atgaatataa acgtattagg
     5941 tacgaacgtt tttaaatgct tttctgggct ttgattttag ggtagggcta aaacatccat
     6001 tgatccaaaa cagctcagaa acgcatttaa acgtatctga taccaatatt acagaaggag
     6061 tgtatcgtat gattaaccta tttcttttag tgacagctag tacattattc tttactggaa
     6121 tatcactgat aatttcctat ttgatctata agaaagccga aacttacaca agcaaagcaa
     6181 ccggactagc cgtttcctcc tttttaactt tgctgacttt ttttgttact ctatcgtttt
     6241 cttttgttta gcacagctga tccatcagca aaaaacctct ctttacatta tacacactag
     6301 tgtgtataat gtaattgtaa acaagctaat taacttttag gaaggagtga caatatgcta
     6361 acagctacat tacagcaacc tactcacgtt gctattgcag aaagctatac aattaatgca
     6421 ggggtcgtta aacagctgat caaggatcga gaattcaaaa agtttaacag tcgtcaagcg
     6481 tataacagac agttaaagaa agctattcta aagcacaaaa attccttcat agggaaattg
     6541 gaaagtgtgg atgttaaaac tgtttcaacg gcattttcag ttgttgaagt gactattgac
     6601 ggtcgcttga tttctctatt tgcttttgga attaattgat agggagtgtg gactttggaa
     6661 ggtaagactt ttgcagaaat ttggactgaa tttattgagg taccaactcg cattgatccg
     6721 ataacttctg aaaaggtatt ggatgcagac ttctacaatc tcaaagcagg tactagcctt
     6781 gaatgcgttt agatgtattt agaagagcat ttcaaaggcg taacgctcgg tcacgtctta
     6841 taccacaatg gtgccgagtg ggatgcaatt ggaaatatcc atttttctta atacaagaaa
     6901 ggagaacaca atatgaatta taccgatgaa gaacaaatca ctttggaata cgttttcaca
     6961 aatgaaaaag gggaggaaat tgaaaaagtc ttgatcattt catcaaaaga cttggagaac
     7021 gctttgactt atgaaccata cgaatcaacc gtaactgtca atgaattctt agatacctat
     7081 acttctaatg atacttggga aattatgaat atttgcaacg ctaataacat tcaatttgct
     7141 gaaaaaacca ataaatcatt ttattagttt tgcaataaaa catcctcttt ttctattgat
     7201 tccatacaca acaatgtgta taatagattt tgtaaaggga gggtagctat gcctaagaca
     7261 gcaagagaaa ttgtaaaaat tctcaaatct aacggcttca aaaagactgg acaaactggc
     7321 agtcatgcaa aattttataa ttccaagact aaaagaacgg tcgttgttcc gatccacaat
     7381 ggtgatgtac cactaggaac agaagctaat atctggaagc aagcagggct ttaacagcct
     7441 tgccttccta tttggtatta ggcatactat cctctcttac actagaaaag gagcgaggaa
     7501 atgaagacaa aaaatatttt ttatccggca atcttcgaga aagaaggaga agcttataac
     7561 atcgttttcc ctgacttacc ggaaatatct acctttggaa atagtcttga agaagcatat
     7621 ttaaacgcta aggatgcttt aggattagcg ctatatagtg taccagatgc agaatttcct
     7681 aaaccatcag cagtggaaga attaactttg aaagccaatc aagtagtagt aatcatccaa
     7741 ctaaacatta aacttttccg aagaagctta aatacaaaaa caattagaaa aaatgttagt
     7801 gttccggaat ggttagttct tttagggaag gagaaaaaca taaacttctc tcaattactt
     7861 caaaaagcac tagaagaaga cttgcttgaa aagtaaaaag gagaccgaac ctatgaaatc
     7921 tacacactta aactatttct ctcgaacgtt aatcaataat ctattaaaag atacaactgg
     7981 cattagcgtt agtaccaaac actttgccaa tgatctaatt atttttgagg ataataactt
     8041 gcgaattgag atgtttattg aacaggcaac tcctaactca atacctgaat tctgctatac
     8101 gcacactgaa aaaatgaaac atgcaaaatc agttaaagat gttgaagcat taagtgaatc
     8161 actaacgaac gctttaaacc tcgttaagaa ggctaaattg ttatttgaat ttcaaaccga
     8221 ctaccacaat gagttgcttg ctcaatctga aattttagat taattgaaac aggagaagag
     8281 aaagatgaaa caaacacaaa cagaacccat taacgatacg cttgctgatc aattagcagg
     8341 ttattataaa ttatttgaga tgagtaaagc acagcgcttg atccactatc tttggaaata
     8401 caaaattttg tttctttttt gggaagcaac actaacattc tcagttcttt tttataagtc
     8461 agatttttat aatcaacact tagttttagg gagttttttg cttatctaca ccattttgat
     8521 gcttaattta tatacctaca tcacacttga agaacactta gtaattagcg atgcagggat
     8581 cgagagaaac atcaaaaggg tatctttcaa gatcacgtta aacaagccta ccgataaaaa
     8641 gctctcagaa gcgttctgag agcttttgac attttcttgt ataatttatc ctaacacatt
     8701 acaaagcacc taattcgagc ttatatcaag caaatttgct atttattcct caatacatat
     8761 ctaaaaatta cttgatggaa aactagatac atggtaagca tgaaaagaaa atcaatttga
     8821 gcagatgcag tataatacat agcaatttaa ggctctatac tttttgatgt tggtgaactt
     8881 caccaacatc ttttttgtat
//
LOCUS       DACCWV010000113         8880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCWV010000113 DACCWV010000000
VERSION     DACCWV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.115"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..713
                     /locus_tag="IVW84_002253"
     CDS             <1..713
                     /locus_tag="IVW84_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7918431.1"
                     /translation="TGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSK
                     EEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYE
                     LKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAY
                     NQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
     gene            complement(795..2567)
                     /locus_tag="IVW84_002254"
     CDS             complement(795..2567)
                     /locus_tag="IVW84_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918432.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(2570..4282)
                     /locus_tag="IVW84_002255"
     CDS             complement(2570..4282)
                     /locus_tag="IVW84_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918433.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4397..5065
                     /locus_tag="IVW84_002256"
     CDS             4397..5065
                     /locus_tag="IVW84_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7918434.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5132..5920)
                     /locus_tag="IVW84_002257"
     CDS             complement(5132..5920)
                     /locus_tag="IVW84_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7918435.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6003..7475)
                     /locus_tag="IVW84_002258"
     CDS             complement(6003..7475)
                     /locus_tag="IVW84_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7918436.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(7605..8798)
                     /locus_tag="IVW84_002259"
     CDS             complement(7605..8798)
                     /locus_tag="IVW84_002259"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7918437.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT         2800 a         1902 c         1471 g         2707 t
ORIGIN      
        1 ttacaggaat gatttttaaa ttcgccaacc gtcgatatgg attaaaatat aatcccgtaa
       61 atggggtttc tttagcaaga aaacctaaga caaacgaaga aataaaaaaa gaaaaagatg
      121 gttatttatc aaaagaagaa gttcaaatga ttgctagtaa acaaaaaagt tcacatcaac
      181 agtcacgtta ttctttaatt actaaatttt tgtttttaac tggactacgt tatggtgaat
      241 tgatatcttt gactgaatct gattatgatg gtaataaaat tacggtttct ggtacttatg
      301 attacgagtt aaaaattaaa acaacaacta aaaatacagg ttcttataga accattgaat
      361 tatcaagtaa tgctaaagaa atcatagatc aattaattga agaaaacaaa ttaattaaga
      421 ataataaaga taaatacatt ttcatctcta aaaatggtaa cccgatatct attcaagcat
      481 ataatcaatc tttaagagca gtatccaaag aattaaacct agataaaaaa gtttcttctc
      541 acatgttaag gcattcccac atttctctac ttacagaatt aggaatccca ttaaaagcca
      601 ttatggatcg tgtaggacat gaagattcaa aaacaacatt aaagatttat actcatacaa
      661 ctaaaaatat gcaaaatcaa ctagtagaaa aactaggaaa aatagaaatt taaattgccc
      721 cttttctgcc ccttataaaa acaaacaaca taaaatatat aaaaagaacc ccgttataac
      781 gaggttcttt taatttaact ttcaaataca aattgattat gataaagttc cgcataaaat
      841 ccattttctg ctaataactc atgatgactg ccttcttcaa tgacttcccc atcacgcaag
      901 acaacgattc tatctgcatt caagattgtt ttcagccggt gagcaatcac aaagctggtt
      961 cttcctttga ttgcttcatc cattgctttt tggatccttg cttctgttac cgtgtctaca
     1021 ttacttgttg cttcatccaa aatcaataaa gatggatttg tgatgatcgt tcgagcaata
     1081 ctgatcaact gcttttgacc tgtactgaat aagttatttt cttcggatat ctctgtatca
     1141 taaccttttt ctaacgtcat gatgaagtca tgaatattcg cttgttttgc tgcattgatg
     1201 acttcttcat ctgaagcgtc cggtttgccg aatgtgatat tgtctttgat cgttccggag
     1261 aacaatacag attcttgcaa aacgattcct acatgtctac gtaatgcatc aagatcataa
     1321 tctcggatat ctattccatc gatgactact gaaccatcat tgacatcgta aaaacggttt
     1381 agcagattca tgatcgtcgt tttccccgat cctgttggac caacaagagc aaccatttct
     1441 cctttgtcca cattgatcga gacatctttc aagactagtt tttctggaga ataaccaaaa
     1501 tctacgtgct gcaacgcaag acctttttca acaccatcaa atgctttacc gttttctggt
     1561 ttgacttcat ctttttcatc aaatatttca ttcaatcgtc ttgcccctgt aatcgctaat
     1621 tggatcatgc tgtatccaga tgaaatctgc atcagtggtt ggtaatattg ttgtgaatac
     1681 tggacgaatg tcacaactaa gcctaatgcc accgaacgtt ccaaatcacc atttaatgct
     1741 aaccagccgc cgaaaaagat aactatcgct gtattgacca gtgacattcc ttgcatcatc
     1801 ggaaatagta gaccagaata agcttgtcct ttgaaggttg cttcgcgaac actttgatta
     1861 tgctctaaga atccttcaat cgtttcttct tgtaagccat ttgtgatgat gacacgttgt
     1921 ccgctgattt tttcatccat ataaccattt agtttcccca cttcatcttg ctgaagatcc
     1981 acatatttcc gtgctttgcg gataatcaag acagctatca aaacggctac tggagtggaa
     2041 gcaattgtag cccaagctaa ctctacattt tggcggaaca tcatgatcaa gatcccgaca
     2101 agtaaaacag cattcgtgat gacttgaagc aaagcttggt tcaaactatt ttgaatatta
     2161 tctaagtcac tggtaaaacg acttaaaatt tcaccgtctt gatgagaatc aaagaaacgg
     2221 atcgtcaatt tttccaattt gttgaataga ccgatacgca ttcggttcgt tgacttccct
     2281 actacttgtg tgaacaaaat actatagata aagttggctg cactagctag tacataaaac
     2341 agcaagagtt tccatattac agaaacaaat ttgcttttat cgtctacgcc ttgcatcaag
     2401 cctcctacgt aattagctaa ttcttgaaaa gcttccccga tgtattgagg tgcctttact
     2461 tgtagatagg tagcaatgat cactgtgata aagataaaga aaaaggagag tttgtaccgc
     2521 ttcaaatagt gatagaaaaa tttacttgct tttattagat cagtcatact tactcctccg
     2581 ttcctttttg agtttcaaag atttcctgat agacacggtt tccagctacc aattcctcat
     2641 gggttccttc gccgactaat ctgccttcat ccaatactaa gatgcggtct gctttgacaa
     2701 cagaagcaat tttttgtgcg atgacaatcg tcgttgttcc cttcaaatct cgatcgagtg
     2761 cttctcggac cagcttttca gagcgagcat ccagtgcact cgtactgtca tctaggatca
     2821 agatctttgg attaccgata acgccacgag taatggataa acgttgcttt tgaccaccag
     2881 aaaagttact gctacgttct tctattggtg cttcgtaacg atcagataac ttttcgatga
     2941 attcttttgc ttgagcaata gaagaagcat gttccatctc tttttctgtt gcattctttt
     3001 tcccttggcg caagttttga gcaatcgttc ctgagaataa aattgctttt tgcaaaacaa
     3061 aggaaaccgt ttgtcttaaa ctcttttcat ttacttgacg aagatctaca ccacctactt
     3121 caactttgcc ttctgtcgga tcaaatagac gagggatcaa ttgtgctagt gttgacttcc
     3181 cggcaccagt cgccccgacg ataccaacca tttctccagg tttgatagaa aagctgatat
     3241 ctttaagcgt atctgtatcc tcaccaggat aacgaaaaga aacatggtca aaagtgacag
     3301 aacccgtcaa ctcttgttct ggaacgtcta aataagtaag atctggttca gcatctaaaa
     3361 tttccgcaat ccgcttcagc gatacggctg ctcttgaagt catcatcatc atcattccgc
     3421 caataatgat tgccatcata atctgcatca agtagttcat gaatgaagca attccaccaa
     3481 ttaacgtagg atcatcttta acaagatcgc tcacaaaaaa gatcgcccca accacagcca
     3541 aattggcagc cagcatgaag gacgggatca tcacagaaaa taatgtaccg actatgatgt
     3601 tgtgttttgt taagtcctca cttactttgc tgaatgactt caattggttt tcttcttgta
     3661 caaaagattt cactacacga atccctagta agttttcttt tgcaatgcta ttcacacgat
     3721 caatcagttt ttgaatgatc atgaagtgct tgcccatcct tgtaaatgaa agtgccgtaa
     3781 tcagaaatac cgtaacgata agcgctacga tgatccacca taattgcggc atcgtgatca
     3841 tagctaaaat gaaggcacca ataaataaga atggaattcg aaaaagtgat tgcaaagaaa
     3901 tcatcactaa attttggatt tgcgtgatat catttgttag acgaacaaca agatttccag
     3961 cagagaactg ttcgatattt ccaaatgaaa atgtctggat ctttcgaaat gcttcttctc
     4021 ggatatctgc acttacgcct tgagctactt ttgcagagaa aatcgtgttc gttacacctg
     4081 cggctaatcc tagcaaagcc agactgatca ggtaaatacc aattgttttc atttcatcat
     4141 tatcttctgt aataatagct tccaatactt gctgtagtaa cttaggttgc cataatgctg
     4201 ctgcaaccat gactgctacg gacagcaagg aaataaaaac agatacttta tattttttcg
     4261 catgtcgtaa gattaatttc attcgttgat tacacccctt tgtttaaatt gacaggacta
     4321 atttaacact gtaaaattag accgactaac aatttaccat gtttaaaaag ataatgcaat
     4381 acacgaggtt gataaaatgg aaataaagct ttctcaagca atcatcattc aggccgcctt
     4441 tgatatcctg gctgaaacac aagctctttc gcagctttcc atgcgaaaca tcgcaaacag
     4501 aattcatgtt caagcacctg ctttatattg gtatttcaaa aacaaacaac agctcttgca
     4561 aaaaatggct gaaacaatgg aagaagaatt agcgcttcca gatccaactt tgccttggaa
     4621 tgaacagttg ctgcagtata tggaaaatta ctatgacctt tatacacgtt tcccttgtgg
     4681 ggcagaatta gaaatcaata ctgtgcccgc ttttccgagt cgattagaac atttggagca
     4741 aatgatccaa ttacttgttc gtgaaggatt ttcagtcacc caaagtcatc aggccattgc
     4801 ctctctccac aatttattga ttggacagtt aatggatcag cagcatgaac agcagcttcg
     4861 acaaaaaatt atggacggaa atgaattttt gaaagaaaca gttctattta tgcgtaatta
     4921 tgtagttgaa aatcaattag agggatttaa agaaagtatg cagtcccatg cagaattgaa
     4981 tgaaaaagca ctctttctaa cgagtgcaca aacctatatt gaaggcttat gccaacaaaa
     5041 taaaagaaga gaaaacgagc agtaaaaaag ctcctataga gatcactatg tttgtgatct
     5101 ctataggagc tatcttttta agactctttt tttatttttc ttccattagc cacgccaatg
     5161 cacgttgaat ccattggtcc cagaagatcc agttatgatc gccaggtcct tgttcaaagg
     5221 tgaccgccaa gccttgttcc tgcatagcag ataccatttc ctgatttgcc tgataaagaa
     5281 aatcagattc cccacaagcc atataaaatc ttggaactgg ttttccgctt tttactagtt
     5341 ctttcaataa gtattgcgga tcattttttg aaccttcgat ttgatccaat ggtccaaaga
     5401 tcccttccca aaaagatgct ttacgaacca tcaaaagatc atccattcga tttcctacag
     5461 aaacagcgcc tgaaagagag attactgctg cgaaattttc tgattttgca aggcctagtt
     5521 ttaacgcgcc atatccgccc atcgataatc cagcggcata tgttttttct ctttttcttg
     5581 ttaattgcgg gaataattca tgacaaatct caggtagctc ttctgcaatg aatgtccaat
     5641 acttcatgtc ataaactgta tctgtgtacc agccaagatc agtagatggc atgatgacag
     5701 ctaatccgta gtttgctaca taacgttcaa tcgacgtacg tctttcccat acactatgat
     5761 tgccacccat tccatgaagt aaatataaaa caggtatatc cgttgtctct tgctctgaac
     5821 tggtgccaat cacctttttt gttgtttgcg gtagtatcac atttagattg acttccattt
     5881 ctaacacatt cgaataaata tttgcttgaa taaatgccat attttcgcct tccttagtaa
     5941 gaagttattt ttgaataact ttaaatttga atgattcggc tacgtacata tcttaaccga
     6001 aactactgtt ttattttacc acttttttca tgaacgaaaa gcgttaagac caaagcaatg
     6061 atcacgagta acaagccaac tagataaacg gaatgtaacc catcataaag aatttttctt
     6121 aatggtaaca aaatatccgc atcaatatat ttagccgttt gtggattcac cagtttattc
     6181 atgatatctg catcatttgt taaatttgct gctgcaagtt ctcgtttagt taccccattt
     6241 atcaataaac caaaaataga aaccatgact gtttgaccaa ttgttcgaac taacgtatta
     6301 aaagaagttg ctacaccaag ctggtcacga gatacactat tttgtgcact gacagtcgag
     6361 gtagtcatgg tcacacctaa gcctataccg atgaccgttg atatagcaaa aaaccataaa
     6421 tagttacttc tgaaaggcat aagatacaac caagcgcccc caatagctgc gattagtagt
     6481 ccaattctca agacccaatg agttttcatt tgagtcatcc acttgcttgc aaggaaagaa
     6541 ccgaccatcc agactagtga taatggtgct aataccagcc caccaattgc tgcattcagc
     6601 cctaatacac cttgcatcca catagggata taaacatcga ttcccatcaa aaaaccacta
     6661 gccaatgctg cagccaagtt gaccacaaca aatagtggat ttttgaataa atgcatcatg
     6721 atgacaggat ctttagctct tttttcggca aaaacaaaaa tacctaacat tacgatacta
     6781 cctagcagca aagcagaaac aagcggactc attccttcgt ctcctaataa ttggaaccct
     6841 aacagcaatg agacaagcat ggccatcaaa ctaaaactac caaaaatatc cattggttta
     6901 gactcatgta tgatttttgg ttcatttaaa tatagccaaa tcaaaataat taatagaatt
     6961 ccgatcggca cattgataaa aaagatccag tgccagctga tcgtttcaac gatgaatcct
     7021 cccgcaagcg gtccaaaaac actggcaatc ccccaggctg aactattcaa acctaaaact
     7081 tttgctcgct tttcaataga ataaagatcg ccgatgatcg tcaaagctac tggcatcatt
     7141 gccccagcac ccatcccttg aatcgcacga gcagcaatca acccaatcat gctttgagat
     7201 aaaccgcaaa ataacgaacc gataataaac aataatgtcc caaaaataaa gattggtttc
     7261 cgtcccactt tatccgcaag ttttccgtaa atcggagtaa gcatagcgtt tgtcagcaaa
     7321 taaatcgaaa atacccagtt catgatttcc attccatgta atgaaccgac aatcgttggc
     7381 atagcggttg ttacgattgt accttcaata gccgtcatga acgttgcaat gaaaatactc
     7441 actgtgacca aagtaacatt tgtttctttt ttcatcttat tcctcccttt cattatgaca
     7501 ggatctaaaa atagataaat aattactatc tttttcaaga ggttaactgc cataaaaaaa
     7561 gctggaacaa ccaccacttg gcgatcgttc cagctgaata taacttattt tgctaaactt
     7621 gctttcaacg catctacttt gtccgtatgt tcccaaggca aatctacatc tgtacgacca
     7681 aaatgaccgt aagcagctgt ttgtttgtaa atcggacgac gcaaatcaag catctcgatg
     7741 attcctgctg gccgaagatc gaagttttct ctcactgcag caatcagttc ttcttccgga
     7801 accgttccag taccgaaagt attgattgag atcgaaacag gttgagcaac accaatcgca
     7861 taagctagct gtacttctgc tttgcgagca agccctgctg caacaatgtt tttagcaata
     7921 taacgtgcag catagctggc agaacggtca acttttgtcg catctttacc agaaaaagca
     7981 ccgccaccat gacgagcata accgccatac gtatcaacaa tgatttttct tcctgttaat
     8041 ccggcatccc cttgaggacc accaatgaca aatcggccag tcggattgat ataatatttc
     8101 gtttgatcat ctaataattc agctggaata acttccttca ctactttttc gatgacatca
     8161 tgacgaattg tttcattatc tactgcatca tcatgctgtg ttgaaataac gattgtatct
     8221 acgcgttccg gttgtccttg atcatcatat tcaaccgtta cttgagattt tgcatctgga
     8281 cgtaaatacg ttaattcatt tgatttacgc aaatctgcca agcgtcgtac cagacgatga
     8341 ctcaaagcga ttggtaaagg catcaattct ggtgtttcat ctacagcaaa cccaaacatt
     8401 aaaccttggt caccagcacc aatctcatct aatacgtctt ttttatcttc atcccggatc
     8461 tcaagtgctt catcaactcc ttgagcaata tcaggagatt gttcatcaat cgcaaccaat
     8521 acagctgctg tatctccatc aaatccgaat tttgcacgtg tataaccaat ttcttttatt
     8581 gtttcccgta cgactttttg aatatctaca tatgccgtcg tagatatttc tccaaatact
     8641 aaaacaagac cagttgttac agacgtttca cacgctactc gtgctgttgg atcttgtttt
     8701 aaaattgcat ccaagattgc atcactgatt tggtcagcaa ttttatctgg atgtccttca
     8761 gaaacagatt ctgatgtaaa taagtgtctt tctaccatta taatgattcc ccctagttaa
     8821 attcggttac aaggcatctc cgtcctgcgg atcctttcgg gaacttgcaa cgaaccaagt
//
LOCUS       DACCWV010000114         8817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCWV010000114 DACCWV010000000
VERSION     DACCWV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.116"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(137..427)
                     /locus_tag="IVW84_002260"
     CDS             complement(137..427)
                     /locus_tag="IVW84_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918438.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IVW84_002261"
     CDS             complement(545..805)
                     /locus_tag="IVW84_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918439.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IVW84_002262"
     CDS             complement(942..1355)
                     /locus_tag="IVW84_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7918440.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IVW84_002263"
     CDS             complement(1811..2296)
                     /locus_tag="IVW84_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918441.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="IVW84_002264"
     CDS             complement(2435..2650)
                     /locus_tag="IVW84_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7918442.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IVW84_002265"
     CDS             2874..3674
                     /locus_tag="IVW84_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7918443.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IVW84_002266"
     CDS             3693..5561
                     /locus_tag="IVW84_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7918444.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IVW84_002267"
     CDS             5554..6045
                     /locus_tag="IVW84_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7918445.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IVW84_002268"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IVW84_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7918446.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="IVW84_002269"
     CDS             6605..7600
                     /locus_tag="IVW84_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7918447.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7742..>8817)
                     /locus_tag="IVW84_002270"
     CDS             complement(7742..>8817)
                     /locus_tag="IVW84_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7918448.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3046 a         1426 c         1683 g         2662 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gttcccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga gtttagtcta atggtgataa
     7621 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     7681 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     7741 gttatttctc tatcacactc cttggtggcg tatagagttg gtgtttacgt agtagcgtat
     7801 ccaccagacg cgtaaatttt cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg
     7861 gaacctcctt atatttactt tgataaaaag ctttgtattc aggaagataa ttttttacag
     7921 agttggcagc ttcaactaag tagtatctaa aatatcgatt tccttgtttt gtcagaggtg
     7981 tattttgcga ctgatagttt ccagattggt tcactttcca acttaatcca gcgtattttg
     8041 ccaattttgt ctgatcttca aatctttcga tttgaccaat ttcagcgatt aaaccggcag
     8101 catagacttt accaacgcct ggaatactgg ttaagcattg atattctgga atgacaacaa
     8161 ttacttgttc gatggcttta tctaattctt tgatattttt ttcaagtgct cgtatttctc
     8221 gtacgagaac acttaaaaca acattaattg attcttgtgc aagagcacct aggcggtaac
     8281 tcatggtaat tgcacgttga attgctttag cgattttttc tggttgcttg aaacggcctt
     8341 ttcctttttc ttgaagtaaa tcacagaaag cttctaaagg aagctcagca agttcatcca
     8401 atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc gtcgattcgt
     8461 ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga aaatgttgct
     8521 ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc tgtaaggcca
     8581 tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag aaatcagcga
     8641 ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta aattgtttca
     8701 cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc tcatcttcat
     8761 gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca atcacaa
//
LOCUS       DACCWV010000115         8674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCWV010000115 DACCWV010000000
VERSION     DACCWV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.117"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1101
                     /locus_tag="IVW84_002271"
     CDS             <1..1101
                     /locus_tag="IVW84_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7918449.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="IVW84_002272"
     CDS             complement(1280..1615)
                     /locus_tag="IVW84_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7918450.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="IVW84_002273"
     CDS             complement(1599..1871)
                     /locus_tag="IVW84_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7918451.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="IVW84_002274"
     CDS             complement(2322..3125)
                     /locus_tag="IVW84_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7918452.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="IVW84_002275"
     CDS             complement(3103..4227)
                     /locus_tag="IVW84_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918453.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="IVW84_002276"
     CDS             complement(4243..5058)
                     /locus_tag="IVW84_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7918454.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5929)
                     /locus_tag="IVW84_002277"
     CDS             complement(5048..5929)
                     /locus_tag="IVW84_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7918455.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="IVW84_002278"
     CDS             complement(6005..7294)
                     /locus_tag="IVW84_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7918456.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7949)
                     /locus_tag="IVW84_002279"
                     /pseudo
     CDS             complement(7438..7949)
                     /locus_tag="IVW84_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8016..8159)
                     /locus_tag="IVW84_002280"
     CDS             complement(8016..8159)
                     /locus_tag="IVW84_002280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7918457.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2692 a         1850 c         1371 g         2761 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac caacgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgg cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattg ataggtttgt gccagcgaaa aaacggccat ttccaatccc
     7921 tcttctttta aatcacagtt aatggtcatg gttgccttct cattggccac tgcttgaaac
     7981 acctgctcaa gggttaccga atctgggaca acttcttacc tgtcatgggc tagtattagc
     8041 gtaccatctg tttgctgacg cacatctact tcaaaacaaa gctggcgatc caaacaaaag
     8101 cgaagaaatg cccagctatt ctcctccgta ttgtcactac cagagtgtgc agtgatcatg
     8161 gtcattttga cccctccttt aattccgtga ttttctccca cacgtccact ataccgatga
     8221 agcgttaatt tcttgttaat ttcttgtaaa ttccctgcgt tttttcagta aactagttgt
     8281 aaatcttgca tgctccttcc tacgaactca tgaatcgtcg attttgccct tctagctagt
     8341 ttcaccaatt aagtagttct acattccccg cttttaacct actacacctt tttttgtttt
     8401 cagctattcc ttcttcttgc tgaaagtcct tgaggttctt tcttacagga taccatcaag
     8461 gtgttcaatg gcttattccc attgaatgac cgagacttac tatcagattt ctgctcgttg
     8521 aaccaagtct ttgatacagg atttcttccg tcatcttctc acaaatacca ccttgcgttt
     8581 ctctagcgat tggtcgatgt gcaccctccc agtggactgt cactacccct ttttgctatg
     8641 cctagcccac aaaaaaagct gtcctcacag acag
//
LOCUS       DACCWV010000116         8498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCWV010000116 DACCWV010000000
VERSION     DACCWV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.118"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVW84_002281"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVW84_002281"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7918458.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IVW84_002282"
     CDS             complement(1098..2201)
                     /locus_tag="IVW84_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7918459.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IVW84_002283"
     CDS             complement(2291..3226)
                     /locus_tag="IVW84_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7918460.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IVW84_002284"
     CDS             3528..5462
                     /locus_tag="IVW84_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7918461.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IVW84_002285"
     CDS             5919..6335
                     /locus_tag="IVW84_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7918462.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IVW84_002286"
     CDS             6349..6915
                     /locus_tag="IVW84_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7918463.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IVW84_002287"
     CDS             6938..7924
                     /locus_tag="IVW84_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7918464.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8498)
                     /gene="scm"
                     /locus_tag="IVW84_002288"
     CDS             complement(8079..>8498)
                     /gene="scm"
                     /locus_tag="IVW84_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7918465.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2508 a         1637 c         1722 g         2631 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt tacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgct
//
LOCUS       DACCWV010000117         8460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCWV010000117 DACCWV010000000
VERSION     DACCWV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.119"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(65..2674)
                     /gene="clpB"
                     /locus_tag="IVW84_002289"
     CDS             complement(65..2674)
                     /gene="clpB"
                     /locus_tag="IVW84_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7918466.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2925..3971)
                     /locus_tag="IVW84_002290"
     CDS             complement(2925..3971)
                     /locus_tag="IVW84_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7918467.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(3982..4254)
                     /locus_tag="IVW84_002291"
     CDS             complement(3982..4254)
                     /locus_tag="IVW84_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7918468.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(4289..5197)
                     /locus_tag="IVW84_002292"
     CDS             complement(4289..5197)
                     /locus_tag="IVW84_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7918469.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(5329..6561)
                     /gene="pepT"
                     /locus_tag="IVW84_002293"
     CDS             complement(5329..6561)
                     /gene="pepT"
                     /locus_tag="IVW84_002293"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7918470.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(6576..7697)
                     /locus_tag="IVW84_002294"
     CDS             complement(6576..7697)
                     /locus_tag="IVW84_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7918471.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(7694..8395)
                     /locus_tag="IVW84_002295"
     CDS             complement(7694..8395)
                     /locus_tag="IVW84_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7918472.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         2329 a         1844 c         1458 g         2829 t
ORIGIN      
        1 aaaaaaggag gtctgacact agttgtgtca gacccctttt ttcttttata gtctcaatgg
       61 aagattagac gatttcttct agctcttctg ttttgaaatg aagttgtcca tcaagtaaag
      121 taatcgttac ttttgattta ggcataacat gtcctgcaac gatttcttta gctagtggtg
      181 tttctacttc cttagtgata aatcgtttca atggtcttgc accatatgct ggttcataag
      241 cattttcagc aatccacgtt ttggcttcat cactgattgt cagcaggatt tcttgatgtt
      301 ccagacgttg tgccaattgc gccaccattt tgtcaacgat tcctttcacg ttgtctaggc
      361 tcaatggtgt aaataaaatc gtgtcgtcga ttcggttcaa gaattctggt ttgaagtttc
      421 cacgtaatag cgtgttgact tgttcagcca ctgcttctgg aatcgttcca tcagcagtca
      481 cgccttccag cagtaactgt gaaccgatat tgctggtcat gatcagaacc gtatttttga
      541 aatctactac tcgccctttt gaatcagtca agcgtccgtc atctaggact tgtaacaaga
      601 tattgaagac atcgggatgc gctttttcga tttcatccaa caagacgatt gtataaggat
      661 ttcgtctgac agcttccgtt aattgaccac cttcttcgta acctacatag cctggaggag
      721 ctccgactag acgagacaca gcatgttttt ccatatattc actcatatcg atccgtacca
      781 tatgatcttc tgagtcaaac aagttctcag ctaacgcttt cgctaattcg gtttttccga
      841 caccagttgg tcctaggaaa aggaaagaac ctagaggacg atttgggtct tgcaatccag
      901 cccgtgaacg aattaccgcg tcactgaccg catcaactgc ttcatcttga ccaatcactc
      961 gtttatgaag tgtttcattc aatttaatta gtttttctcg ttcaccttct acaagtttcg
     1021 ttacggggat acccgttaat ctgccaacga cttgagcaat ctcattttcg gtaacggact
     1081 cttgaaccat cttaatctcg ctgtcttttg ctttagcttc taattccttc aattcttttt
     1141 ctaattgagg tatcgtacca tgacgcaata cagcagcgcg ttctaaatca tagttatttt
     1201 ctgcatcttc taattcgtgt tttgctttat cgatttcagc acgttttgct gaaacagaat
     1261 tgacttcttc tttttctgtt tcccactgca tcttcatcgc atttgcttct tcacgaagtt
     1321 cagctaattc ttcttgcaaa tttttcaagc gttttttact tgcatcatct gattcttttt
     1381 tcagtgcagc ttcttcgatt tccaactgca tcaaacgtct agtaacttga tctaattccg
     1441 ttggcataga gttcatttct acccgaatcg ttgcactggc ttcatcgatc aaatcgattg
     1501 ctttgtccgg taagaaccga tcagtaatat aacggtcaga taaagtagct gctgctacta
     1561 atgcgttgtc atggatgtta acaccatggt ggatctcaaa acgttctttt aatccacgca
     1621 gaatactgat cgtatcttcc actgttggtt ctttgactaa taccttttga aagcgacgtt
     1681 ccaacgcttt gtctttttcc atatattgac ggtactcatc taaagttgtg gcaccgatca
     1741 aatgtaattc accgcgagca agcattggtt tcaacagatt tccggcatcc atacttcctt
     1801 ctgtttttcc tgcccctacg atattgtgga tttcatcgat gaacaagagg atacgtccat
     1861 cacttttttt gacttctttc aagactgctt ttagtctttc ttcaaattca ccacggaatt
     1921 ttgctccagc gatcaatgca cccatatcta atgaaaaaat cgttttatct tttaaatttt
     1981 caggcacatc tttgcgtacg atacgttgag caagcccttc aacgattgcc gttttcccta
     2041 caccgggttc tcctatcaga acaggattat tttttgtttt tcgtgaaaga atacggatga
     2101 catcacggat ttcttcatca cgaccgatga ttggatccat ttttccgctt ttgacttgct
     2161 gtactaaatc aacgccgtat ttttctaatg ctttgtattg ttcttcttgg ttttgtgagg
     2221 tcactctttc tcctcctctc atctcttcga tgtttttacg caattctttt tcagataatc
     2281 cgtttttatt caaataaaca gttaacggat aattttttaa tttcatcaac gctaaaataa
     2341 cgacttccgt tgaaagaaac tcgtcgccga agctttcacg tatttggtct gcttcattca
     2401 ataatcgaaa gaaattctgg ctgaggtttt gtccatattg gacgtttcca ccagaaacaa
     2461 ctggatactc atccagtaga cgatcaattt catgttcaaa agattcgaca tccaatccgg
     2521 catccgtata gaaatttcga ccaaaatgat taggttgtaa aaagattttc catacatggg
     2581 caatgtcgat atcttgatgt tttcgagtga ccgcaatctg ttgagcttca gcaatcgctt
     2641 cttgaagcgt cgtagtcatt ttctcaatat tcatacaggt ataccctcct aattaaaggt
     2701 aagatcactt atttctttga tctttacaaa aactattata tcgctttggt caattttggt
     2761 caaaatatac acatggtttt ttgacttttt ttctcgactt atataaatct taaaaaaaca
     2821 aaattgattg tatgtatata aaaaaagagc caaacaaacg taaaattatc ttatccataa
     2881 cgtttttctt tgatttgata aaaagggtag atcttataaa agacctaccc ttgattttct
     2941 ttattctctt tttttacttc tattatttct tctttgatca ctcttcgccg ttcagcaatc
     3001 acatacacaa atccatagag aaatggcaag ataaaggaac tgaaacgata cagtaaaagc
     3061 gaagaaactg cgtcaactgt tccaacaact ggtctgaata ataacaaata gacaaattca
     3121 aaggaaccaa taccagcagg cgtaggaata acccctgaaa gcactactgc aaagctgata
     3181 aacgaaatgg tcagtagaaa atctatttta ggatggtttt caatcagaat caaataagga
     3241 agtacatacc aaaatacaag ttttaacata ttccatagat aaatccgcaa tagcgcagtc
     3301 ctgtcttgga taatggtttg gaccgtttcc cttaaagagt agatctgctg attgcatgta
     3361 tccacccatt tccgcaaacg ctcactttta aataacttat tcgataagct gacaaaaaat
     3421 atttgaacat tcaagctgac ggacatcgcc aataaaaagg caacaatcac gatcgtcaaa
     3481 aggataccag cgatgataaa ccagatcatt gatggaccat tagaataaaa aagagagaat
     3541 tggatgacca agccagctaa tgcataagtg atcactgcca gtttgtacat aatcatatgg
     3601 agtgctgtca cccctgctcc ctgggatagc ttcattcctt ttttgttata aaaataaatc
     3661 tccgagatca gcgttcctgt accaaacgaa acgattcggt aaaacgcaat atagctagag
     3721 gtcaagaacc catctttcgt tgtaaaattc ggcgcgaaag gttcagcaat ttctttgatt
     3781 gagcgccctt cagcgatttg gtaaactgtc cctaatataa cagccgctgc caaaacaaca
     3841 acactggtag acagcaattc ggcaaagata tctgcaaatg attgattgat taaataataa
     3901 ataatggcaa aaatcacaat cagcaaaagc agattcagaa tgattttagt tttcgttcct
     3961 ttagacgaca tctcctgctc cttaatggaa aaaataaatg agaatcaaat caacaagtac
     4021 acctgcagcg aatcctatag taaacttctt tttccgagtt ttatgatgga aaacttttcg
     4081 agagatcaat cctcccaaac caccgcctaa caggctgaca aatatcaaat tattctcagg
     4141 aacacgccag ccgctagttc tagcctgatg tttatcatac cccatcagac aaaataagta
     4201 aaaattcaca atgaaaaggt aaaaccacaa aagattgtgt aacaatacat ccattactct
     4261 tcttccaact ttctgttaac tactatttct aagctttttc ttctttcttt ggtgaataaa
     4321 ctgttaatcc ttttgataca acagataatt caactggaag atcatctgtc gtgtctccat
     4381 ccgttcgagt gcccacagat ttttcctgcg cttggatttt catgcgtttg gctgtgaaat
     4441 aagtaatgcc ggggatcgta tagaatttgc ctctcatgat tctaggcaaa taaaaaataa
     4501 atcggaaaat atctaaggaa ggtacgatcg ttacatgaaa ttttccatca tctagggtcg
     4561 ctgagtcgtc aaaatttgta aaaccgccca cggagttaga catcgtgact gtcaacagtt
     4621 gggattttcc tttccaatgc tcctcgtctg tttctaaatc caaatgatat tttttcttct
     4681 tcagcaataa cttaaaaaac ttcttgataa agatccacga gccgtaacgc tgtttttctt
     4741 cttgactgat ccaaactgct gtatctgcta agattccgat tgtcagtgta gagataatga
     4801 cttcatcatt gacttgacca tagtctattt tccgtacatt gccttccagg atcacgtcag
     4861 cagcttcttc ttttttgagg ggaatctcaa gaacacgtgc aaggttgttg acagttccac
     4921 ctggaagtaa tccgacatgg tattgagata atgttttttg gaacgcttgg acgatatgat
     4981 gaatcgtgcc gtctccacct ataattacta aagtatcagc ttctatggct tctgctttct
     5041 tgactaattc cgcatcatca atcgatgggc cagtttcagc tagttctaca tataattctg
     5101 gctgcttttc ttttacataa tcttgaaacc agcgtgctaa tttctcaccc tcatctttcc
     5161 ctgaactttt gttataaaaa actagcagct tttccattct ttacctccta tttactcttc
     5221 aaaaaagagg gcgggacaac caattccatt gtcctgccct ttagccttag acagatgaca
     5281 ttccatcatt gggacgaacg ccttgtcata agctgttctt tttcgatttt aactctttgt
     5341 gttcaattct gcaattttta caatcacgtc tgttgctttt tccattactt gtaaagaaac
     5401 aaattcgaat cgtccgtgca tattttctcc tccggcaaat agatttggcg tcggaatacc
     5461 catgtaagag atttttgaac catctgttcc tccacgaact ggttcgatca ctggagagat
     5521 accaagctcg atcattgctt gtttcgctaa ttccacgatg ctcatatctt tttcaatgat
     5581 ttctttcatg ttgtaatatt gatcatacat atctactttg attcgttctt gatcaaatcg
     5641 ctgattcaac tcttcttgga tatgtgtgat ttgtgctttg cgtgcttcga atttttcacg
     5701 atcatgatcg cgaatgatat aactcataga agcttcttca ggattcccat ccaatgccat
     5761 caaatggaag aaaccttcgt atccttccgt tttttctgga acttcatcgg ctggaagctg
     5821 attatgaaaa tcgattgcta actgtagagc attgatcatt gtatcttttg ccgtccctgg
     5881 atgtacattt ttcccttgga tcgtaatatt tgcttgagca gcactaaacg tttcatactg
     5941 caactcacct aaaggaccac catcgactgt ataagcaaaa tctacatcga attgctcaac
     6001 atcgaattta tccgctccga caccgatttc ttcatcagga ccaaaagcca ctttgatggt
     6061 accgtgaggg atagaaggat tattgatcaa gatctccatt gcagtcatga tttccgcaat
     6121 ccctgatttg tcatctgctc ccagtaatgt actgccatct gttgtgatca atgtgtggcc
     6181 ttcataattt ttcaaatttg gaaaatcttt tgtattcaat gtatagcgtt cttccgcatc
     6241 tagtacgatc gtagactcgc catcatagtt ttcaatgatt tgtggattga ctcctactgc
     6301 attaaaatct gctgtatcca tgtgggaaat aaatccaatg gaacgcactt catgatcaac
     6361 attgcttggt aatgttccca taacgaatcc gttatcttca ttatagaaaa catcagctaa
     6421 gcctaactct tctaattctt ttttcaattc ttgcgcaaaa gcaacttgtg tctgagtcga
     6481 tggagtcgtt ttgcttgttg catcggagcg tgtttctgtt ttgacatatc gtaaaaaacg
     6541 tggcaaaaga ttttcgtaca tagtattctc tccttttaat taaattgaaa tgggtttgta
     6601 tttgtttcag aaacgaaaaa gttaatatcc caattttctt cttgtttcca agattcaaat
     6661 ttctcgataa atcgttgctt gcataacgat tcaatataat gaccgggatc aattgcaatc
     6721 aacccagcgc tttgcatatc atgagccgta tgataataga tatctcccgt gatatagaca
     6781 tctgctcttt gggcgattgc ttgcggataa aatttttcac cgcttccacc gcaaatcgca
     6841 atgcgcttca cagaggattt tgcatttttt ggctggacta ttctcagtcc atctagttgg
     6901 aacgcttctt ttacttgttc tacaaatgcc tctatagaaa tttcttgcgg caattcacct
     6961 actcgtccta acccaaacat ttcgactggt tcatctatag cgaaaagatc ataagcaggt
     7021 tcctcataag gatgagctga ccgcatggct tgaatcactt gtttttcgat agtttccggc
     7081 aaaataacct caacttttgc ctcttgaact tgttctgtct tccctacttt cccaatcgct
     7141 ggttgtgcac cagcttccgg agtaaatcga ccgtgtccaa tcgaggtaaa acttgtaccg
     7201 gtataatcac cttgtgtacc agcaccagct gcagctaata cttcacgcat tttctgtgca
     7261 tgatccacag gtacataaac ggccagtttt ttataatgaa tctcatgagt ctttactaaa
     7321 taactctcca cttcaatacc gagaagttca caaaaccagt cattcagtcc atcccaaata
     7381 atatccatgt tcgtgtgtgc tgcatagacg gcaatatcat gtttcaacaa atctgcatac
     7441 attttttctt gtggctgatc agtaaccagc cgttttacag gacgaaagat tggaggatgt
     7501 ttagcaatca gcaaatcgat ttttttcttg attgcttctt cgaccacttc tggacgaacg
     7561 tctaatgtca tcattactcg ttggattggc ttatccaatg ttccgatatg cagtccgact
     7621 gggtctcctt cttcagctag ccactgagga caatagcttt caaacttttg gatgaaagtg
     7681 cgtccgctga ttgtcatggc tttaaaacct cctcaatcat ttggatttcc tgctcgattt
     7741 gtttttgacg tgcctcttga ttagctgctt gtttgagctg ttcacggata gcttttcttt
     7801 gttgaagctc tcttgtccat tttgatttga agatctcact tttctcttca agcagaaatg
     7861 gcccgaacat cagctccatt tcattgtaag taactggttt ttgttctttt tcggctacaa
     7921 tgatttcata tattttttta ttttcttcaa gtattttttc tgattggatt ttatactgat
     7981 tttcttgcag ccaagatcgt aatgtacgct ctccgatgtt tggctgaagg atcaaacgtt
     8041 cttgtccgct taagcgttga ttcaacttgc cagcttctag gatatctcgg atcagtgaac
     8101 ctcccatacc cgcaatcgtg acagctgtaa taccatcttg tggctggata gcatctagac
     8161 catttgcaag gcgtacggtg atacggtctt ctaatccatc ttttttgact tgcttacgtg
     8221 ctgattcata aggaccttga acgacttcac cagcaactgc atattcgatt tttcctctta
     8281 atatcaatgc aacgggtaag taagcgtgat ctgatccaat gtctgctaaa cgagcggatt
     8341 cagggacaaa gtcacccact gcggctaagc gctgagataa atctaaatgg ttcatagctt
     8401 cactccttct agataattat agcattttcc aaataaaata aggagactag gaataaaaaa
//
LOCUS       DACCWV010000118         8398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCWV010000118 DACCWV010000000
VERSION     DACCWV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.120"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(35..532)
                     /locus_tag="IVW84_002296"
     CDS             complement(35..532)
                     /locus_tag="IVW84_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7918473.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(662..1372)
                     /gene="budA"
                     /locus_tag="IVW84_002297"
     CDS             complement(662..1372)
                     /gene="budA"
                     /locus_tag="IVW84_002297"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7918474.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1385..3049)
                     /gene="alsS"
                     /locus_tag="IVW84_002298"
     CDS             complement(1385..3049)
                     /gene="alsS"
                     /locus_tag="IVW84_002298"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7918475.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3254..3916)
                     /locus_tag="IVW84_002299"
     CDS             complement(3254..3916)
                     /locus_tag="IVW84_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7918476.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3926..4741)
                     /locus_tag="IVW84_002300"
     CDS             complement(3926..4741)
                     /locus_tag="IVW84_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7918477.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5003..5446)
                     /locus_tag="IVW84_002301"
     CDS             complement(5003..5446)
                     /locus_tag="IVW84_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7918478.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5580..5918)
                     /locus_tag="IVW84_002302"
     CDS             complement(5580..5918)
                     /locus_tag="IVW84_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7918479.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5906..6283)
                     /locus_tag="IVW84_002303"
     CDS             complement(5906..6283)
                     /locus_tag="IVW84_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7918480.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6507..7700
                     /locus_tag="IVW84_002304"
     CDS             6507..7700
                     /locus_tag="IVW84_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7918481.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7866..8288)
                     /locus_tag="IVW84_002305"
     CDS             complement(7866..8288)
                     /locus_tag="IVW84_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7918482.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2463 a         1695 c         1414 g         2826 t
ORIGIN      
        1 tgatttctct tcttttttgt ttcaatagac gattttagtt attaataaat gaaaaagcat
       61 ttatattaaa atttgctaat aaaagatcta catatttttc tagaaatgct tgatcgattt
      121 catcataccc tttcgttact ccgcaatcga tatctaaaac accgattaaa ttaccttctt
      181 ttaccattgg cactacgatt tctgattgtg cggctgaatc gcaggaaatg taatttgcat
      241 gggttttgac atcatcaacg atcaatgttt cttgtttttc agctgattct ccacaaactc
      301 cttttcccat cttgatacgt gtgcaagaaa ctcgcccttg gaacggtcct aaaatcaatt
      361 cattaccatc gaataaataa tatcctgcaa agacagtctc aggcaaagtc tcattcaaca
      421 atgcagatga atttgctaaa tttgctaacc agttatcttc tgcttctaat aatccttgtt
      481 gctgtaataa taaaatttcg tatgcatctt ttttctgttt ttctgtccac atgataaaaa
      541 ccgctccttt ttgatctatg gagaagatta tagctcagtt tttttgtaaa cgctaggttt
      601 tctcctcaaa aaaacgagat acgtcaggtt aatcaccttg aacgcatctc gctgtctttt
      661 tctattctgc ttgttggatt tctgctgcta aatcagcata atcaatcgtt tcgtgaacaa
      721 aagtcggatc atcaactgga aaatgctgta caagggacga gatgcggcta atctcaactt
      781 ttccttcagc cacttcaaaa tccattacat gtccgccaaa tgtatgtgta tcatcgataa
      841 aatgcaggtg aaatcccgct gcagcaactc cttggaataa ttcaggcgta aagaacccga
      901 ctaatgttcc ttggacattg ctttgttgga attctggttg agactctgaa atttttgcta
      961 gtcttctgta aggtttttct tgcttcggca tgatacggat gtgcatattt ttgaataatc
     1021 cagttatctt cactgccaaa aataggtttt ccccggcttc tcttactaat atgtcttttt
     1081 tcaattgttc tccatctgca gtttttgaga cagaaaaact agtatctggc gtaaaatctg
     1141 taattgctgc atagggggta agttcacttc catctaattg ggcaactgag ccatctgatc
     1201 ttccttgata agctacgcca tctaaaaaga tgacttcacc atcaagccca tgcaatgttc
     1261 ccaatcccag agttcctttt tctaatatat gagcaatcgt ttctgttcca tccatcaagc
     1321 cagccatcaa agctcctaat gtcccgtgct ggtataagat tttttcagtc atgtcgacac
     1381 ttccttaata gaattgatct ggtaagattg tttctcccaa tttctcatta tcacggtaat
     1441 caatcggaat atcaataatt acgggtcctt ctgtcgcaaa tccttcttct aaagcttttt
     1501 ctaattcctc tacacttgta gctcggattc ctttagcgcc aaaagcttct gcatatttca
     1561 caaaatcaac tggaccaaag tctacacctg acgcccgctg atatttcatt ttttcttgga
     1621 actctaccat attgtagtgt ccatcattcc aaatcaaatg aacgatattc aattttttac
     1681 gtaccgccgt ttctaaatcc tgggcagaga ataaaaatcc cccatcgccg gaaactgata
     1741 cgatttgtgt atttggacgc accaatgcag cagaaatcgc ccatggcaat gcaacaccaa
     1801 gcgtttgcat accattactg aataacaaat gacgaggttc ataacttcta aagtggcgtg
     1861 ccatccaaat ataatgacta cctacatcta cggtaacagt catatcatct gttacttttg
     1921 actgaagcgt gttaataact tctaaaggat gcaagatacc tgcttctcct tttgattgaa
     1981 caatgtcccg ctccgttaat tctgcttgaa gagatgctaa atattctttt gcatcttctg
     2041 acacttgttg aggctctaat gaacctgtca ataaatcaag agtggctgag atatcaccga
     2101 tcaattctct ttcaggctgc ataaaaggat cgatttcagc tggcgcttca tcgatcacaa
     2161 tgatccgcgc atctttttcc gcattccagt ttcttgcttc atattcgatt ggatcatagc
     2221 caatagcaat aactagatca cttcgtttca aaagcatatc tcctggttgg tttctaaata
     2281 aacccacacg accaaagaaa tgcgcttcta attcacgtga gatcactcca gctgcttgga
     2341 atgtctctac aacaggcaat gctgtctttc caactaattg cctgatggct aacgtttctt
     2401 tttcacttga tcctcgcata cctactaata gtaccggtag ttttgcttct ctaatgcgtt
     2461 cagctaggta acggatatca tctgcagaag cagaacctaa tttaggatcg ctcaatggct
     2521 tgatgacatt cccttggaca ggtgcatcca ctacatcttg cgggatactg ataaagctcg
     2581 cgcctttttt tgaactctta gccatacgat aagcattggc aatcacttct gaaattgttt
     2641 ctggatcttg gatttctgca ctgtattttg tgattggctg gaacagtgct gcattatcca
     2701 tgctttgatg ggtcaacttc aatagatcgc ttcgttttac ttgtccggca attgctaaaa
     2761 ctgggtcacc ttctgctgtc gctgttacca aacctgtagc taagttacta gcaccaggac
     2821 cacttgttgc gataactact cctggctctc ctgtaatacg tccgattgct tgtgccataa
     2881 atgcagcatt ttgttcgtga cgagtcacga ttaattcagg accttgatcc tctaattcat
     2941 taaatacacc atcgattttt gcacctggta taccgaatac ataatcaaca tgatgattaa
     3001 tcaaactttc tactacagat ttcgaacctt gtttcatttt ttcgtccatt gaacatcgtc
     3061 ctttttatgc tttattttta attattagtt agaaataatc acaaataatt gatacagttt
     3121 ctaaacctgt ttcattcaaa agtatagcac gttttatttc atttgtttag ccatatgttt
     3181 caaaaaatga aaaagaatga cagtttttac ataaaaactg tcattcttaa ttgaaagttt
     3241 caatttataa agtctacaga tccatagttc tcatcaattt acgattgttg aagtaaatca
     3301 aaccaaatac gatagaagga cctagtagaa tacaccattg gggctttacg aaaaagaaga
     3361 gaaacaaact tgtcaatagt aaaaaatcta gtaaactcac ataccatttt ttcaacgtat
     3421 agtagaacgc tatccgtaga acttgatttg gtttcgacgc tggatttctc acctgaaaat
     3481 accagtagtt gatgctaaag gaaactgata aacaagcaag taaaacaaac aacggaatca
     3541 accattttcc ataagaaaaa cgtataaaaa agaaacaatc aaaaatcatt atcacactaa
     3601 ctatccaagc aaatagccaa taaaaaaatc cctttagcca aaattggcga aacgtcttta
     3661 tgaacaaacg tactggtgaa aaactttttt cttttgctaa ttcttctgtc atacctatca
     3721 tcacgatgca acctggacca ataaaaagta aagaaaaaat aaaggaaaag acattccttc
     3781 catctatcga caaaaggaac gcagctgcta tatacaagat attgacacac caaaaacaaa
     3841 ggctacttgc caatgattga tacaaccaat aaaagaattc catataaaca tttttgtcaa
     3901 aacgctgctc tttcataatc acctcctatt ccttcattcc tgatgaagcg atcccttcta
     3961 caaaatattt ttgcatcgct aaaaatataa aagcaacagg aagaacagaa acaactgagg
     4021 cagccatgat taatgcgtac tctgcatcaa atagcccaat aaacattttc aaccctaact
     4081 ggattgtttt attttccgta gaagacaaat aaatgaatgg ccccatataa tcgttccatg
     4141 tattaacgaa tgtgatgatc gtcaaactgg caatcgccgg ttttgtaaga ggtaaaatga
     4201 tcttgcgata gattccccac tctgataagc catcgatccg cgcactctca cataaagaat
     4261 ctgggatact actgtagtat tgcttcaaca agaacacacc aaaagcattg aacgcttgta
     4321 atagcaccaa cgaccacaac gtatatgtta aacccacttt tctcatcata atgaactgcg
     4381 ggatcatata agactgccaa gggacagcaa tcgtgccgat atatgctaaa aataaaatat
     4441 cgcgcccttt aaaatttagt ttagaaaacc cgtaagcagc aaaactagaa gtaaacaact
     4501 gaatcaacgt gatcacaacg cttaaaaaag ctgtattctt aaaaaatgtc actaaaggaa
     4561 tacgagacca aataaccaga taattctcta aatgccaagt tttagggatc cattgaatcg
     4621 gaatcgtgaa cacttcatta tttgctttga atgaagcaga aaccatccat aaaaaaggaa
     4681 tgatcgtcaa taaagctaat acaatcagca gaatcatacc caaccattga ataaagggca
     4741 tgttttttga acgaattttt gctttttttc tttcatttcg aacacctcca aagttataat
     4801 cttcatttct ttgtaatgat tacaatccag aacaacggtt attatagctg attcatttcc
     4861 gttcttctta tgtttgtaag cgcttattta acaagttatg taaatctctt tatttttttt
     4921 gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt tgtattgaaa
     4981 tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg tgaatcaatt
     5041 cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag ttcgtttcaa
     5101 tcattgggtc aagcggaacg aatgtcactc gctttgaaag atgacttaca gcaccttcta
     5161 tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt
     5221 acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca aacattcgct
     5281 gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct tttgttatca
     5341 cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt tcctttcttg
     5401 gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc gctaaatcta
     5461 acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg atatgaaatt
     5521 gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta ttcgaattat
     5581 cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt ctgttctcga
     5641 attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt ttttagaaaa
     5701 taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc tgttcctagt
     5761 tcctcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg tagaacttct
     5821 gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag taagcgtaac
     5881 tccatagcga tgctccttga tttttttact tgatacattc cattttattt ctagataaca
     5941 cacgatcata tttcttgatt tttccttgta aacgttccaa acattcttgc atttcagcaa
     6001 tttttttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca aatacagttg
     6061 cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat aatccggcat
     6121 ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa tcacggattc
     6181 cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag cgcaatgtat
     6241 cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca ctctttctct
     6301 taatttcttg ccactttact tatgtatttt atttattcta caactttaag tgtactccaa
     6361 gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt ttgtgtatta
     6421 tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta acaatttagg
     6481 gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaagtta ttattatcga
     6541 catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt tcattatctt
     6601 caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca acgatcacta
     6661 atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa tatggacgtg
     6721 tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg atgtattttg
     6781 cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga gcgggaggag
     6841 aatacggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa attggacgag
     6901 cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg attttagctg
     6961 ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt
     7021 tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat
     7081 ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt gcttggcaaa
     7141 catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg ttaatgaatt
     7201 ggctgccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct tcattgtgga
     7261 tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata atcatggata
     7321 aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta tctgtctatc
     7381 tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata gtcggctact
     7441 ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat ccaacagaaa
     7501 tccgcgcgac ggcgaataat ttcattatga accttggacg agctgttggt ggtttttctt
     7561 ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc ttgtttttga
     7621 gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt acagcattaa
     7681 gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg tgtggcaaaa
     7741 gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg tttccgtttg
     7801 gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct tctatctggc
     7861 ggctactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt agcataacga
     7921 aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt tttctcagga
     7981 tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa atgaagataa
     8041 ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt attctcattc
     8101 ttcaaaatga ccttatcagg atatttttgg aacaattctt ctaaatctgt tagataattc
     8161 aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga acaccaacgt
     8221 tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt tgttaaaaaa
     8281 gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga aaaaaaacgg
     8341 atacaaatat catattgtca caccgtcaat agtgcataaa cctgttatta tttctctt
//
LOCUS       DACCWV010000119         8332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCWV010000119 DACCWV010000000
VERSION     DACCWV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.121"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(423..920)
                     /locus_tag="IVW84_002306"
     CDS             complement(423..920)
                     /locus_tag="IVW84_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7918483.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(960..1769)
                     /locus_tag="IVW84_002307"
     CDS             complement(960..1769)
                     /locus_tag="IVW84_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7918484.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2103..2960
                     /locus_tag="IVW84_002308"
     CDS             2103..2960
                     /locus_tag="IVW84_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7918485.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2953..3855
                     /locus_tag="IVW84_002309"
     CDS             2953..3855
                     /locus_tag="IVW84_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7918486.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3888..4238
                     /locus_tag="IVW84_002310"
     CDS             3888..4238
                     /locus_tag="IVW84_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7918487.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4272..5210
                     /locus_tag="IVW84_002311"
     CDS             4272..5210
                     /locus_tag="IVW84_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7918488.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKITPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5288..6181
                     /locus_tag="IVW84_002312"
     CDS             5288..6181
                     /locus_tag="IVW84_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7918489.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6219..6782
                     /gene="amaP"
                     /locus_tag="IVW84_002313"
     CDS             6219..6782
                     /gene="amaP"
                     /locus_tag="IVW84_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7918490.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6796..6984
                     /locus_tag="IVW84_002314"
     CDS             6796..6984
                     /locus_tag="IVW84_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7918491.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6997..7899
                     /locus_tag="IVW84_002315"
     CDS             6997..7899
                     /locus_tag="IVW84_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7918492.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7931..8173
                     /locus_tag="IVW84_002316"
     CDS             7931..8173
                     /locus_tag="IVW84_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7918493.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2887 a         1456 c         1633 g         2356 t
ORIGIN      
        1 taaccgtccg gtcgtaggtt cgagtcctac aaggtacata agaggttgtg aaaaagctgc
       61 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt tacttggctg
      121 ttcttattac tgcagatgca agcttttgaa taccgtttaa ggaagaggtt gtgaaatcag
      181 cgttttgacc tgagtattag cttaacaaac ggaaaaatag ttcctcatat tttgccgttt
      241 gttaagctaa taccgagggt cagctgattg aacaccattt aagaaagagg tcgtgagcct
      301 gccgtttcac tctgtgtcat aaaaaagcca atctactttg atagaaaaag cacgtaggac
      361 taaaacagtt ctgcgtgctt tttttattgt gtgttaaata tcgtaaatga agatagatat
      421 ttttatgatt cgacgataga gacgttgaaa tctttcaaca gctctaaagc ggattctccg
      481 tgttttctac tggagactaa cattggatca acaaaaaagt ctgcttcagg aaaagcactc
      541 tgcaggccta tcagattaga gagtacacac aagtttgttt caatcccgac tacttcgatc
      601 tctttttgtt cttctttgct ttcaaataaa gtttcctgga tttcagctaa agtttcaggt
      661 ggaatcgtaa agtaatactt tttaatcact cgttcatttt cttgtggaga aagaagaggg
      721 atcaattgca aatcttctcg ttcttcttct ttgtccttca gttcaactgg tatatcccgt
      781 gtgaagagaa tatattcatt attttggcgt gctttagcta atctttgagc aatacgttct
      841 gccaattgat ctgactctgg aatataaaat tcgctgtttg gatcaagaat atgattttgc
      901 atatcaataa taactaacat tttctttcct cctacattca tatcgtcatt ttattctttt
      961 taatagaagt tttttaaaat ataagcaaca ccagcttctt cattcgtcag tgttacttgg
     1021 tttgcaattt cttttacttg tcgctgtgca ttccccatcg caatactcaa cccgctgtaa
     1081 ttcaacatag caatgtcgtt ttcttgatcg ccaaaagcca tgatgtcact cgattcgatt
     1141 ccaaggtaat ccgccagaaa aacaagtgaa tgagctttag tgatacctgt aggcgtgatt
     1201 tctaagaaat gcggatcaga gaatactgat tggataccat ctgcttgcag atctctttgt
     1261 agcttctgta gtttttcttc actcttatct gttaatgcta atttaagata gctaagagaa
     1321 ggatcttcca acatatttaa aatttgatca aaagaatggt tctgtaaatt gaaatgttca
     1381 taataagggt catctttgac tagatttgat tcatcgaatg tcagctgatc atcttgggtt
     1441 tctcggctag caatgatgga tattttcgct tcttgagcat agaagaaggc ttttctcagt
     1501 gtatcgctat ctatttttga acgtctgaga actttcgcca tttttccatc cttcatttca
     1561 gcaatcagtg aaccattgga agaaatcaaa tagccaccga ttcctaattc ggaaaaaata
     1621 tctttagcag aaatgctgtt ccttccggta gcgacggtta cgattccgcc attttcatca
     1681 aatttccgaa gtgcttggat attttcttct ggtatctttc catttcgatc aagcaaggta
     1741 ccgtctaaat caatcgctgc tagtttcatt ttatcttcct actttctttt tacatatcta
     1801 ttttcctgtt accttaagat taaatgagaa tttaaaaaaa tgaaactaac ttgctttgtc
     1861 attatgaatt ttttcaaaaa acattcttaa gaaaaaggtg gctatatata gtagtttttg
     1921 gtcaatacct ttgaataaaa agccatgaaa aacgaatagt tagcgcttac cgatgcgttt
     1981 tgtgataaaa aaattttaaa atattcaatg aaaacaaagc atatattttg ctttaaaacc
     2041 aaatgaggaa tattctagat gtagaacagg ttagaaaaag attagaaaaa ggagtgattt
     2101 tgatggagaa gagatacggc gtagttcctg tggtaactgc ttcagacgaa aattatgctc
     2161 cttacttgag cgtgatgatc gcaactgcat tagaaaattg caataaaaca agacgcataa
     2221 aattttatgt gattgacgat ggtctatcag aatatagtaa agaaggattg gaagaaacgg
     2281 taaataaata ttcaagtaat gcaagtatcc aattcctgac agtggaaaaa gatatctacg
     2341 aagacttcct agtcagtgac catatcacaa ctaccgctta cttaagaatt tctttaccaa
     2401 acttattagc taaagaggat tataagaaag tcctttactt agattcagat gtgttagtac
     2461 tagacgatat cgtaaaacta tatgatgagc cgttaaatgg aaaaacaatc ggcgctatta
     2521 tcgatccagg ccaagtgaaa gctttggaaa gattaggaat cgattccgat gacttgtact
     2581 tcaattcagg agtgatggtg attgacattg atcaatggaa taaaaaagaa attacagaaa
     2641 aaactatcca ttacctcagt gaaaatggcg atcgaatcat ctatcacgac caagatgctt
     2701 tgaatgctgt actttatgaa gattgggaac aacttcatcc aaaatggaac atgcagactt
     2761 cgctgatttt tgaacgtcat ccagcaccaa atgaaaaata tgaacgtcag tataaagaag
     2821 gaaatgaaaa accttcgatc gttcacttta caggacatga taaaccttgg aatacgttga
     2881 aagatcatcc gtataccaat ttatacttaa aaaaattagc acacagtact ctaacgaaag
     2941 taggcgagat caatgaataa aaaagaaata gcagtagtcg caagttgtaa cacaaaattc
     3001 gtaccacatt tagctgcatt atttgtctca gtattagata attgcaaccc gtctaaattt
     3061 gttcggttct atgtgattga cgatgatatc gattttgaaa gtaaacagct tttacgcttc
     3121 tcagtgaaaa acgcacgaat gaactcagat gtggaattct tgaaaatcaa taaagaattc
     3181 tttacaaatg tggtaatcag cgatcgtatt ccagaaacag cttattatcg tattgcgatt
     3241 ccggaattgt tccgcggcac agaagttgaa cgtatcttat atatggattg cgacatgatc
     3301 gctttacaag atatctctaa attatggcgt cttgacttcg gtgattcaat cgtagcagct
     3361 gttgaagacg ctgggttcca tcaacgactg gaaaaaatga aaatttcagc taaatcaatg
     3421 cgttatttca actcaggttt gatgctgatc aatgtgaaaa aatggttaga tgaaaatatt
     3481 acgcaaaaag tattagattt catcgaacat aatccagaaa aattacgatt ccatgaccaa
     3541 gatgcattaa atgctatctt acatgaccgt tggttaccac ttcatccgcg ttggaatgca
     3601 caaggctata tcatggcaaa agcaaaaaaa catccaacag cagcaggtga acgtgaatac
     3661 gaagaaactc gcaacaatcc ttacattatc catttttcag gacatgtgaa accgtggagc
     3721 aaggattttg aagggccaac gaagaaatat tacgaaaaat atgcagggat gacggctttc
     3781 cgttgtgtag ctaaattccc taaatatcca aaatatgcaa aagttcaacc aaaaacagat
     3841 gtagttgtta aataatagaa gacaacaact gaagaggggg agtaggaatg gaaattacaa
     3901 tgtctaaaaa tgcagtagag acgttaattg aaaaggttgg ggaaaataca aaaatcgcac
     3961 tcgcgcttat caatgattcc gatccatttc taagggacaa aggtgctttt gcaaaaggca
     4021 gcttttttca aatcattcct tttgtttcgg aatttggaga atatgcgact aaaatcgagc
     4081 atccattatt ggacatctat acatctaaat tagagcaaaa ttatttcggt aaacgattga
     4141 acatggactt caacaaacag cttgacagct tttcattaga aaatgaaatc gcagtactcg
     4201 attataatat caaattaaag aactgcttct tcagctaata gtttcgagaa agtccaaaaa
     4261 ggaggtatac attgagatac tactttgtgg atgaagaaaa gaatgtaata gaaacagatg
     4321 aaaaaaaata taattggctc gtacttgata gtgcaaagcc cgaagagatt gagcaagcaa
     4381 tcgaagaatt cgagcttcct gatgatattt tcgtaggaac cgaatatccg gaagaagttt
     4441 ctcggatgga gcgcctttat gagacgaaac tagagcatcc attttctttg gtagtgatta
     4501 atctagatca gtcagataat cgtatcgaaa aaaaaataac gccaatatct tttgtacttt
     4561 ccgataatct cttgattact tgtatcgata gaaaaaccga atttgtggat cggctgctga
     4621 caaagcatgg aaatcattta aactcgtttg aaaagatcat cacgtataca ttattagata
     4681 tttacaccca ttacgtgaaa gaactaagag agatgaaaaa gcgaattgac gctttggata
     4741 aagcagctcg taaaacaacg gaaaatgaag aactgtataa acaggcagat cttgaaagag
     4801 acatcgtata tatcgatcat accttaagag atcaacggga aacaatggat caattatgga
     4861 attctgaaac tttcatcaaa cgtttagacg atgaacagct actctatgat gtccatttga
     4921 gacagcgaca aacagaaaaa atgatcgaga tctatcgaga tttattagag agtattggtg
     4981 atctattcaa cgggatgatg gacaataatc taaatcatat catgaaatat ctagattcag
     5041 ctgcattaat catttccgtt ccggcgttgt tcgcagggat ttggggaatg aatacaggag
     5101 gattaccggg taaaagttct gctctaggct ttatactcgt agtctttggc tctttgatag
     5161 cagcagtagt tttaggttac catttatacc ggaaagatta tacaaattaa agaatctatt
     5221 taaaacaggt atcaaacaaa gaaatttgtt ttggcaatac aatttaaaaa ctaggaggaa
     5281 tacagcaatg acagaaccaa aattaatcga cccgcgaaac ttgtaccata cggagaaatt
     5341 tcctaaacaa gatcaggata caccagcttt acagcagaat atgattcctg ttcctgattg
     5401 tggagaagaa tcctatgaag gcaataatca actagaaaac cgccgtgtac taatcactgg
     5461 tggagattca gggatcggtc gtgcagcagc gattgcattt gctagagaag gggcagatat
     5521 cgctcttcaa ttcttcccag gagaagaaaa agatgcagag gaagtagcaa aatacgtgaa
     5581 agatgcagga cgtaagattg tcttactacc ttttgatctt cgtgacgaac aagcacctaa
     5641 agaaatcata gataaagccg tgagtgaact tggcgggcta gatacgatgg tactaaacgc
     5701 aggacagcaa atttcttgtc agtctattgc tgagttacca cttgagcaag tcaaagacac
     5761 atttatgata aacatcatcg ctatgtttgc tttagtgaaa gaagctgttc cgcatatccc
     5821 agcaggagga gcaatcgtaa caacaacttc tgttcaagca ttcaatccaa gcgaacattt
     5881 attagattac gcagcaacaa aagcttcaat cgctaacttt acgatcggtt tagcaaaaca
     5941 gcttgcacct aaaggaatcc gtgtaaatgg tgtagcacct ggtccaatct ggacaccatt
     6001 acaactagac gaaggacaac cttctgattc gatccctgaa tttggtcagc aaagtttact
     6061 agaacgtgca ggtcagccag gagaattagc accagtttat gttttcttag catcgaataa
     6121 agcaagttat gttacagccc aaatttacgg agtaactggt ggagaagcga tcaacttata
     6181 agaagttgat cgtttcaccc ctatgaggag gtgcgaaaat gaataaaggt gtaaaaacga
     6241 tcggagtgat cggaacatta cttttgctat ctgtgctttt gtttgcgata ctattaagtg
     6301 caacctatcc attgcctttt cagctagaga agtttcgatt cttttccatc actaattcat
     6361 acgtgcaaca atatgtattt tggcttgccg ttgcatttgc tattttgaca attatcacgt
     6421 tacttgtttt cctgttttat ccaaaaacaa tcggaaactt tctactgaaa catggtgatg
     6481 gggagttgac gttggataaa aaagcaattg aaggaatggt tcgttctcac ttgcatgaag
     6541 atgaattcat ccattcacca aaagtaaatg tcaaagcaac gaaaaacaag atcgccgtca
     6601 atgtaaaagg ggagttgaaa cgaacttcag cactcattgg taaaacagga acattgatga
     6661 aagaaatcga aaatgaagta caacacatat tgggaacaaa agagccagtg aaagtatcgg
     6721 tgaaatacac aggctttgaa gaaccagcta aacgaagcaa tgaccgcaaa cgagtagaat
     6781 agggggggac caaaaatgaa agaactattt tcgacataca agctccctat catctttggg
     6841 gtacttggat tgatattagc cgttttatgg atcaccgtgg gctttttcaa gaccttattg
     6901 atcttgatca tggcagcgat cggcgtcgct atcggttttt atctgaaaca aacgaaaatc
     6961 ttagatcact attttaaatc ttaggaggaa acatccatga ataacaataa caaaaataat
     7021 aacaataaaa tgaacaacca aaaaccagtg tctgcacatc cagacccaac gacaccaaac
     7081 caatcaaatg gtgtgaacga tgaaaagaac aaaacagcac cagtatctgc acatccagat
     7141 ccagtagtcc ctaacgttca acaaggagta gaagagaaga aacatgatgc agaaaagcat
     7201 gcacctcatc atccaggaac tccagaatct aaagttccag aacaacatga tgcaatcgat
     7261 ccttctaaac ctgtggaaga gcaaaaacat ccttctgacc atgcgaaagc acaagaaaaa
     7321 cttagttctg accacacaaa cggacaagga aaacttggtt ctgatcatac aaataaccaa
     7381 ggaaagcatg attctgaaca ggaagattca atgcgcggtg agctgaatta tgaagataaa
     7441 gttgtgcaaa aaatcatcgg aatcgcaatt gaacaagtag acggtttatt atctgcaaac
     7501 ggtggtttct tctctaatgt ggcaggaaaa cttgtgaata cagataatgt aacggctggg
     7561 atcgaaacag aagtcggtaa aaaacaagta gcagttgata tggatgtcat tgtggaatat
     7621 ggcaaagata tcgaaaaaat ctttgaagaa atgcaagaag taatcggcaa agaagtaaaa
     7681 aatatgacac atttagaagt gatcgaagtc aacgctaacg tagtggacat caaaacaaaa
     7741 gaagaatttg aaaaagatca agaaacggtg caagacaaag taactgacgc agcgaaaaaa
     7801 accggtgaat ttgcttcgaa tcaaacagat aaagtaaaat cagcagctgg aagcggcgca
     7861 caaaaagtaa aagaaaatac agaaccacgg gttcagtaat aaggaagtaa aacattagga
     7921 ggaacgaaac atgggattta tttggtcact tatcgtcgga ggaattatcg gagcaattgc
     7981 aggagcaatc acgaataaag gttcttcgat ggggattatt gcaaatgtcg tagcaggttt
     8041 agttggatca gcacttggtc aagcattatt aggttcttgg ggaccaagta ttgcaggaat
     8101 ggcaatcgtg ccatcattga ttggtgccat cattttagta ctaatcgtat cattctttgc
     8161 tcgaaaactg taaaaagact tttaaaaaga ggtcgtgaaa aagctgttct tcatcaagta
     8221 ataagaacag ccaaacaaaa atagcttctc atatttttca tttggttggg catattatcg
     8281 aagatgcaag cttttgaaca ctgtttatac ggaataagag gctgtgacaa ga
//
LOCUS       DACCWV010000120         8058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCWV010000120 DACCWV010000000
VERSION     DACCWV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.122"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            18..797
                     /locus_tag="IVW84_002317"
     CDS             18..797
                     /locus_tag="IVW84_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918494.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            843..1787
                     /locus_tag="IVW84_002318"
     CDS             843..1787
                     /locus_tag="IVW84_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7918495.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKNALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1803..2582
                     /locus_tag="IVW84_002319"
     CDS             1803..2582
                     /locus_tag="IVW84_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7918496.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2594..3292
                     /locus_tag="IVW84_002320"
     CDS             2594..3292
                     /locus_tag="IVW84_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7918497.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3320..4084
                     /locus_tag="IVW84_002321"
     CDS             3320..4084
                     /locus_tag="IVW84_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7918498.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4128..5579
                     /locus_tag="IVW84_002322"
     CDS             4128..5579
                     /locus_tag="IVW84_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7918499.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5611..6747
                     /locus_tag="IVW84_002323"
     CDS             5611..6747
                     /locus_tag="IVW84_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7918500.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6747..7853
                     /locus_tag="IVW84_002324"
     CDS             6747..7853
                     /locus_tag="IVW84_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7918501.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7859..>8058
                     /locus_tag="IVW84_002325"
     CDS             7859..>8058
                     /locus_tag="IVW84_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7918502.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKH"
BASE COUNT         2891 a         1227 c         1706 g         2234 t
ORIGIN      
        1 tacttaggag tgaatctatg gaaaagccat tcgtcaagtc attgactgct gtggcggtag
       61 gtgcaggtgc tattgcgatc tgtttgtttg gataccatat caataatcag cggcaacatc
      121 agcaacggat caattatgca gaatctgcca tcaccaatca aaaagacacc ttgacttctt
      181 tatcaaaaga agtagacaag ctgtatagta caaaagaaaa aatttttcta aaccctgaaa
      241 taacagaaga aacaatcaca agtttgtcaa ataagctatc ctctgtcaaa ctttcagcag
      301 atgattacga catcaaagaa tcagaattgc ctaaagaagc tgttgcaatc caagatgaga
      361 agaaagcaat cgtgacacag ttagatagtg cagaatcaaa actgaagatc cagacagctg
      421 tcaacgattt atttactaaa aatgtatcaa actggcagca agctgtagac gatgtcatca
      481 tcaaagaaaa ggtgacaagt gcagatgtcg ctcgtgtacg tgaaaatatg agctttttta
      541 aagatagtcc ttggaaaacg gttgttatgc aatatctagg attcgctgat attcagatcg
      601 cgcaagtcac gcaactagat cagttattcg atacgatgtt aaaggatggt caagtgactg
      661 ccaccgctac ttacgatcaa tatttgacgg tgctcagtca aatagagcag atccggaatg
      721 aaaaaataag cgcagcatac gcaacaaaag cacaaactgt tgctgagcaa atgggctata
      781 gcaatataag ctattgacag ttaaaacgtt gattcaagca aggttagcaa acggaggaaa
      841 ctatggcaag acgaagaaga aaatcaagag ggaaaaaaat atttctgatt attttagcag
      901 ttcttatcgt attagtatta gcaattgtag gagtaggagc aaaattatat ctggatctct
      961 cacattctat tgagaaaact tacgaatcag tggaacgaac ccaaggagaa cgaacgaaac
     1021 cagttgatct ggacaaacaa gaatctttct ctgttctatt gatggggatt gatactggag
     1081 atttaggtcg ggtcgatcaa ggaagatcag atacgatgat ggttgccaca gtcagtcctg
     1141 aagacaaaca gacaacgatc gtcagcattc ctcgtgatac gtatgtcgac atcgtaggtc
     1201 gtgacacaaa agacaagatc aaccacgctt acgcattcgg cggagctgcc atggcgatgg
     1261 ataccgtaca aaaatatgta gacatcccaa tcgatcacta tgtctctatc aatatgaaag
     1321 ggctaaagga actcgttgat gcagttggtg gtatcgaagt caataatgac ctcactttct
     1381 cacaagatgg ctatgacttt ccaatcggta aaattacatt gaacggggat caggcattat
     1441 cttattctcg tatgcgttat gaagacccaa atggtgatta cggtagacaa gggcgccaaa
     1501 gaaagatcgt agaaggcgta gcgaaaaaag tattgagtct aaacggtgtc agcaactatc
     1561 aatccgtttt gaatgctcta gagtcgaata tgaaaacaga catgaacttt gatgaaatga
     1621 aaaaaaatgc tttagattat cgggaggcat ttggtacgat taagcaagat cagctgcaag
     1681 gagaaggctt catgcaagat aatatctcct atcaaaaagt cagtgatcaa gaacttgatc
     1741 gtgtgaaaaa agaattaaaa gatcaaatga atctagagaa taaataggaa agtggagaaa
     1801 aaatggaaga gacaatcagc ttgaaagaaa tttttgaaac attacgcaaa cacttggtaa
     1861 taatcatcat ctcaatgttt gctggcttgg ccatctcagg aatcgtaaca tttttcatct
     1921 tgacaccaaa atacagttca caagcacaat tgatcgtgac actgccacaa tcagacactg
     1981 caaatgtcaa tgatgtcaat acgaacctgc aaatgatcaa tacgtataaa gatatgatcg
     2041 tcagtgattt agtgctcaat gaagtcaaag atcgtttaga aacagaagac aatgtgaaaa
     2101 tgactgctga acaaatcaaa gatgccatct cagttaatca ggcagaaaat tcacaaatgt
     2161 tctcgattca ggcaataagt acgaatgcag tggctgctca acagatcgct aacacaactt
     2221 cacaagtttt ccaagagaac gcaaaagatg tgatgaatgt tgataaaatc tcgatcattt
     2281 caggtgcagt tgccaatacg acacctgttt cgccaaataa taaattgaat ttaatgactg
     2341 gcttaatgtt aggaatgatg gtaggtgtag gtctggcatt cttattggaa ttactcgatc
     2401 ggacagtgaa agatgaaaaa tatgtgacag atacattagg attcccaatc ttaggaacag
     2461 ttccagaaat gtcagcgaaa gaattgaatg ctggtatccg taaaaaagga ccagctcaga
     2521 cacaaccaaa agctacaaac gataaaacag cgccatcacg cagaagccgc tcaagagtct
     2581 aggaggaaag aaaatggcac gaacacagaa acaaaaagca gcccaaactc atgcagtcag
     2641 tttggtcaca ctcaccaatc cctcttcacc aatcgctgaa caatatcgta cgattcggac
     2701 gaatatccag tttgcctccg cagctggtca gcaaatcaaa acgattgttg tgacttcatc
     2761 aggacctgga gaagggaaat cgaccactgc agcaaacatt gccgttgttt ttgccaaatc
     2821 tggtcaacgg gtgctgctag tcgatgcaga cttacgtaag cccgtcatct ataaaacgtt
     2881 caagttgaac aacgcaagcg gattaagtac tgttcttagt tcatcaggaa gtgtagcaga
     2941 tgagatccaa cgaacaccag tcgacaattt gtcgattctg ccaagtggac ctaaaccacc
     3001 aaatccatca gaactactaa gttcgccaag aatggatcag attttagcag aagcaagaca
     3061 actatttgat gtagtgatct tcgacatgcc gccagtcgtt gccgtaacag atgcacagat
     3121 catgtcttca aaaacggatg gcaccttatt ggtcgtacgg gaaaatacgt cacgaaaaga
     3181 atcattgact aaagcaaaag aactgttaga tatggtacaa gcacgagtct taggtgtcgt
     3241 atacaacgga gcagaacaca gcaaagatgc tggttattac tacggaaatt aaggaataag
     3301 aagagaggag acaagagaaa tgatcgatct acattgccat atcctaccag gaatcgatga
     3361 cggggcagaa aacttagaag caagtattgc aatggctgaa aaagcgattc agcaaggcat
     3421 tacgcatatt ttatgcacac cacaccacaa taatggaaaa tacagcaatg aaaaatcaca
     3481 agtcatttct ctagtcgctt cgttacaagc cgaattagag aaaagacagt tgcctttgac
     3541 gttattggaa ggacaagaag tacgtattac cggaacgctc atcgaagaca tccaccgaga
     3601 tgaaatttta tttacagatt tagatgatac atatcttctg atcgaatttc cgacactgga
     3661 agtgccgtta tatgcagaac ggcttttttt tgagttgtta gaactaggaa agataccagt
     3721 gatcgtccat ccagaacgca acgcgcattt tcgagaagac ccaaatcatt tgcttccgtt
     3781 cctagatatg ggatgtttgg cccaacttac tgcgccaagt tatgttggtg tatttggcaa
     3841 agatatccag aaaacagcca aaacaatggt caaacataat ctcgtgcaga tggctgcttc
     3901 tgatgcacac ggggtctcta agcgcacctt ttacttaaaa gagtgctatg agcagattgc
     3961 caaggagttt ggtaaggaaa aagtagtaca gatgaaacaa gtcgcaaagg atctgatcaa
     4021 cggggatgag atcaattatc caatctatga agaggtaaaa aagaagaagt ttggtttatt
     4081 ttagttcttt attcgtagat aaaaaggaaa tgaggagaga gtgagagatg tatagaaaaa
     4141 actcaagtgg ttggtctaaa catattgatt ttattttatt agacttattt tgtgtccagc
     4201 ttgcttttta tatatcatat gtaatgcgac agggtgactg gaatccgtat gtgattccta
     4261 tttatcgcca catggcaatt tttatagaac ttgagaatct tacgattata tttttatttg
     4321 aggcatataa aaatgtttta aaaagaggat attataaaga ggctgtagct tctataaagc
     4381 aatcttttat gctgttactt gtgtgcagtt tataccttat tacaatgcag gatggaaatg
     4441 attattccag agtttcttta tttataatgg gggtggtgta tggattatta acatatataa
     4501 ttcgtatact ttggaaaggg ttgttgcatc accgcatgaa aaacggtgaa gatgtatcgt
     4561 tagttattgt aacatctcaa aagattgcaa aaaatgtagt tcgtaatctt aaagagaaga
     4621 attatggcat gtataaactt agtggtgtga ttgtagtaga cagagatatg tatggaacta
     4681 ccatcggagg tgtcccagta gttgcaaatg ctgataatgc agcggaatat cttttacaga
     4741 attgggtaga tgaagtattc atcaatgttg acgaaagtgt tccatatccc aaagaattaa
     4801 tcgccagatg ttcggaaatg ggacttgctg tacatatgaa tcttacaaaa gtgtctgata
     4861 gtacacgtgg aaaacaattt attggccgag ttggtgatta tactgttatt acatctagta
     4921 ttaatgttat gacgatgagg caagcattta taaagagaac gattgacatt cttgctggaa
     4981 ttattggatg tattgcaaca ggtgttattt ttatcttcgt agcaccagtt atttacatca
     5041 aatcaccagg gccgattttc tttgcacaag aacgtattgg tcaaaatggt aagacattta
     5101 agatgtataa gttccgtagt atgtatatgg atgcagaaga acgcaaagca gagttgatga
     5161 aagaaaataa gatgagtaat aatctcatgt ttaagatgga ttttgaccca agaatcattg
     5221 gcaataaaat acttcctgat ggaacaaaga agacaggaat tggacaattt attcgatcta
     5281 catcattgga tgagtttcca caattttata atgtcctaat gggaaaaatg agcctggtgg
     5341 ggtttagacc ttgcttgaag tcagaatatg agaagtatga ttttcatcat cgtgcgagaa
     5401 ttgcgatgaa acctgggatt actggaatgt ggcaggtgag tggtagaagt gatatcacag
     5461 acttcgaaga ggttgtaaaa cttgatgcag aatatatcag gaattggagt atgggattag
     5521 attttagaat tttgtttagg acagtcaaaa ctgtgcttca tagaaacggt tccatgtaag
     5581 ataaagatac tatagaagga gtaaaacgtg atggcaagaa aaaaattaaa aatctgtatg
     5641 ctaggtcata aaagaattcc atcaagagaa ggtggaattg agatagtggt agaagaattg
     5701 gcaacgcgaa tggttgcact tgggcatgaa gtgacttgct ataatcgaag tggtcatcat
     5761 gttagcggaa aggaatttga ccagcataaa aataaagaat ataaaggtgt aaaattaaag
     5821 actattttta cattaaatat aaaaggaatt gcagcaatga gttcttcagt atttggtgga
     5881 attagagcag cattcggaaa atatgatatt gttcattttc atgcagaagg accatgtgct
     5941 atgttgtggt tgccaaaact ttttgggaaa agatgtgttg caacaattca tggtctagat
     6001 catcaaagag aaaaatggaa caagcttgct agtacataca tcatgttagg ggaaaagtgt
     6061 gctgtaaaat ttgcagatga aataattgta cttagtgaaa gcgtccaaaa ttattttgaa
     6121 gatatatatg gaagaaaaac aaggtttata ccaaatggcg ttaaaaaaat tgaaataaaa
     6181 tcagcaggat tgattacaga aaagtatgga ttaacaaaag attcttatat tttgtttttg
     6241 gggcgattgg ttcccgaaaa aggaattcgg tatctaattg aggcatttaa agatgttcag
     6301 acagataaaa aacttataat agccggagga agctctgata cagatgaatt tgcaaatgaa
     6361 ctaaaggaat tagcaaaagg tgatgagcga attatattta ctggatttgt acaaggacag
     6421 gaattagaag aattatatag taatgcgtat atctatacac ttccaagtga tttagaagga
     6481 atgccattaa gtttgttaga agcaatgagt tatgggaact gttgtatagt ttcaaatatc
     6541 tcagagtgta cggaagtagt agaagacaag gctatgattt ttaagaaatc ggatgtaagt
     6601 gatttaaaga taagattaga agaggcatgc aatcaatcgg aaatggttaa agttttgaag
     6661 aatcaagcta ctgagtttat ttgtagtaaa tataattggg ataaaatcgt acaagaaaca
     6721 ttaaatttgt ataggggaaa tatataatga ttcgaatatt acagtgtgtg aatgatatgc
     6781 atcgtgcagg gttggagacg atgctaatga actattatag gaatatagat cgaactaaaa
     6841 ttcagtttga ttttttgaca catcgtccat ataaaagtga ttatgatgag gaaataataa
     6901 gtcttggagg aaaggtctat tatgcaccga gactttatcc acaaaattat cctaaatatt
     6961 ttaaatggat ggaacaattt tttaaggatc atccagaata tgagattgtt cattctcata
     7021 ttgatgcgat gagttattta cctttaaaag ctgcaaaaaa agctgggatt ccgatacgga
     7081 tagcacatag ccataataca tcgatagata gagattttaa atatctttta aagcaatatt
     7141 atcgatgtaa gttaacgtct gttttgacac atgaattttc atgtggaaaa gtagcaggtg
     7201 attttttgtt tcgaaatgat aaattcactt tgattccaaa tgcagtggat gctaaaaaat
     7261 tttattatga tgaaagtatt agaaatgatg tacgaatgaa attaggaatt tcaagtcaga
     7321 cgtttgtaat gggacatatt ggaagaattt catattctaa aaatcatcgt tttttgattg
     7381 aaatttttaa agaatttcac gctattaaga ataattccgt attattaatt attggaactg
     7441 gtgatatgga agaagaaata aaaaattatg caaagaaaag tggcatagat gatgatatta
     7501 aatttttagg aaatagaaat gatatagaaa aattttatca agcatttgac gtattaatgt
     7561 tgccatcatt atttgaaggg gttccgttag tgggtattga agctcaattt gcagatttac
     7621 cgtgtttctt ttcggagaaa gttcctacag aagttgcttt cagtgataag accaatttca
     7681 taagtttaaa tcaatcggca aaagaatggg cgagagagat tgcaaatgta gatatctctc
     7741 atcgagattc agaacgttcg attttaataa aggcagatta taatattcaa acagcataca
     7801 aaatcttaga atcaaaatat tatgagttat ttgaattaat acaaagaggt taaagaatat
     7861 gacacagaaa cattcaagta taaaaagaat tatacatcgc atatccgaag aattgaatgc
     7921 aacaagacga attgcaaaga taacatcttg gaaggaagca tttgccacgt tacaagctaa
     7981 aattgatatt caagtcatga atcgtaatgg atataatgaa agtaaaaaac agaaggaaca
     8041 tttattaaaa aaacatga
//
LOCUS       DACCWV010000121         7907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCWV010000121 DACCWV010000000
VERSION     DACCWV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.123"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(71..1492)
                     /locus_tag="IVW84_002326"
     CDS             complement(71..1492)
                     /locus_tag="IVW84_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7918503.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="IVW84_002327"
     CDS             complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="IVW84_002327"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7918504.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3903..5159)
                     /locus_tag="IVW84_002328"
     CDS             complement(3903..5159)
                     /locus_tag="IVW84_002328"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7918505.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            5739..7040
                     /locus_tag="IVW84_002329"
     CDS             5739..7040
                     /locus_tag="IVW84_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7918506.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7109..7708)
                     /locus_tag="IVW84_002330"
     CDS             complement(7109..7708)
                     /locus_tag="IVW84_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7918507.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2366 a         1602 c         1377 g         2562 t
ORIGIN      
        1 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
       61 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
      121 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
      181 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
      241 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
      301 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
      361 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
      421 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
      481 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
      541 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
      601 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
      661 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
      721 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
      781 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
      841 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
      901 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
      961 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
     1021 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
     1081 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
     1141 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
     1201 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
     1261 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
     1321 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
     1381 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
     1441 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
     1501 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
     1561 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
     1621 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
     1681 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
     1741 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
     1801 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
     1861 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
     1921 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
     1981 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
     2041 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
     2101 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
     2161 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
     2221 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
     2281 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
     2341 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
     2401 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
     2461 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
     2521 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
     2581 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
     2641 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
     2701 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
     2761 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
     2821 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
     2881 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
     2941 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
     3001 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
     3061 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
     3121 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
     3181 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
     3241 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
     3301 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
     3361 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
     3421 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
     3481 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
     3541 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
     3601 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
     3661 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
     3721 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
     3781 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
     3841 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
     3901 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
     3961 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
     4021 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
     4081 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
     4141 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
     4201 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
     4261 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
     4321 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
     4381 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
     4441 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
     4501 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
     4561 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
     4621 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
     4681 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
     4741 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
     4801 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
     4861 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
     4921 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
     4981 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
     5041 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
     5101 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
     5161 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg tgtaattaat
     5221 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
     5281 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
     5341 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
     5401 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
     5461 cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc
     5521 aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat
     5581 tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc
     5641 aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg
     5701 ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta
     5761 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
     5821 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
     5881 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
     5941 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
     6001 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
     6061 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
     6121 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
     6181 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa
     6241 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
     6301 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
     6361 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
     6421 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
     6481 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
     6541 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
     6601 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
     6661 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
     6721 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
     6781 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
     6841 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
     6901 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
     6961 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
     7021 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
     7081 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
     7141 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
     7201 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
     7261 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
     7321 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
     7381 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
     7441 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
     7501 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
     7561 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
     7621 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
     7681 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
     7741 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
     7801 agctagcaat tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta
     7861 ctaaaataat aaaaaaaagt tttataaaaa ggtataaaac cttttta
//
LOCUS       DACCWV010000122         7833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCWV010000122 DACCWV010000000
VERSION     DACCWV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.124"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IVW84_002331"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IVW84_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7918508.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IVW84_002332"
     CDS             complement(2203..4389)
                     /locus_tag="IVW84_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7918509.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IVW84_002333"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IVW84_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7918510.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IVW84_002334"
     CDS             complement(4611..5051)
                     /locus_tag="IVW84_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7918511.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IVW84_002335"
     CDS             complement(5125..5664)
                     /locus_tag="IVW84_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7918512.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="IVW84_002336"
     CDS             complement(6279..7745)
                     /locus_tag="IVW84_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7918513.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2127 a         1725 c         1371 g         2610 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgt
//
LOCUS       DACCWV010000123         7830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCWV010000123 DACCWV010000000
VERSION     DACCWV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.125"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            79..780
                     /locus_tag="IVW84_002337"
     CDS             79..780
                     /locus_tag="IVW84_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7918514.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1389..2405)
                     /gene="tsaD"
                     /locus_tag="IVW84_002338"
     CDS             complement(1389..2405)
                     /gene="tsaD"
                     /locus_tag="IVW84_002338"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7918515.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2402..2866)
                     /gene="rimI"
                     /locus_tag="IVW84_002339"
     CDS             complement(2402..2866)
                     /gene="rimI"
                     /locus_tag="IVW84_002339"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7918516.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2873..3415)
                     /gene="rimI"
                     /locus_tag="IVW84_002340"
     CDS             complement(2873..3415)
                     /gene="rimI"
                     /locus_tag="IVW84_002340"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7918517.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3399..4223)
                     /gene="tsaB"
                     /locus_tag="IVW84_002341"
     CDS             complement(3399..4223)
                     /gene="tsaB"
                     /locus_tag="IVW84_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7918518.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4312..5292)
                     /locus_tag="IVW84_002342"
     CDS             complement(4312..5292)
                     /locus_tag="IVW84_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7918519.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5316..6800)
                     /gene="galT"
                     /locus_tag="IVW84_002343"
     CDS             complement(5316..6800)
                     /gene="galT"
                     /locus_tag="IVW84_002343"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7918520.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6812..7801)
                     /gene="galE"
                     /locus_tag="IVW84_002344"
     CDS             complement(6812..7801)
                     /gene="galE"
                     /locus_tag="IVW84_002344"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7918521.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2190 a         1693 c         1391 g         2556 t
ORIGIN      
        1 gttggtatgt taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa
       61 taaggagagg actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga
      121 tatcaacctg attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact
      181 gttcttcgtt cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg
      241 ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat
      301 tactactatg actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa
      361 gagacctaca cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt
      421 tcctactatg aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa
      481 atttctaaag tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc
      541 tttgaccggg taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa
      601 gatggtcata tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc
      661 acgacacgtg aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat
      721 tatgacaaaa agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa
      781 cgcgaacaaa aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc
      841 taaaacttct gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat
      901 gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg
      961 aagcgtcttg tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact
     1021 cagtgagggc aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa
     1081 caataatgat taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat
     1141 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
     1201 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
     1261 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
     1321 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agctttttca
     1381 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
     1441 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
     1501 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagctgccaa agcatcctgc
     1561 aatccttcac gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact
     1621 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
     1681 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
     1741 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
     1801 atcgctcgcg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
     1861 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
     1921 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
     1981 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
     2041 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
     2101 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
     2161 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
     2221 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca
     2281 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
     2341 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
     2401 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
     2461 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
     2521 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
     2581 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
     2641 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
     2701 tctggttatc ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
     2761 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
     2821 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
     2881 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
     2941 tgatcgttgt tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca
     3001 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
     3061 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
     3121 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
     3181 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
     3241 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
     3301 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat
     3361 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
     3421 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag
     3481 cgaggaagaa aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca
     3541 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
     3601 tcctttattt cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
     3661 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt
     3721 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
     3781 aacatgggca caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta
     3841 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
     3901 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
     3961 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
     4021 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
     4081 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
     4141 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
     4201 gcaaaactag tacaataata catgattatc tgctggcggg caaattttgc cccttcctga
     4261 aacacaaaaa agctgcggta tcactccatt ttcccacagc ttttccatct tttatttcgt
     4321 tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg gtgctttgga
     4381 attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg tataaacttt
     4441 tacagtagaa agcgagggcg ttacatattt cgctacactg acatcattga agcccatcac
     4501 agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc
     4561 taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt ttaaatattg
     4621 cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg gaccttcgtc
     4681 gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt ttagccgctg
     4741 atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg ctttatggga
     4801 gacaaagtgt tcaatgactt tttgaacact tgacttaaag tcgacaacta gagagtcgat
     4861 tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc ctttcatttc
     4921 taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgccg gaagatcact
     4981 aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct ttttttcgat
     5041 tcctaggcga atagataaat aataaagatc ttcaagctct tccgtatcgt cgtaccactg
     5101 gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg tataatttaa
     5161 gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa gatcaggatc
     5221 atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg caatgtcttt
     5281 aattgttgcc atttgtctct cctccttttt tacgtttata aaatcgcagt aaatcgttca
     5341 aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag tacacgtgca
     5401 aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt caaatcagga
     5461 tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg gttttcttct
     5521 cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg taaaacagca
     5581 agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac atctttatgt
     5641 ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag gacaaggtcc
     5701 aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca
     5761 tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca tttttccaat
     5821 actgagttag ctgcttcaat aagcaagttt ttctctgttg attgcaaacg gatcacagac
     5881 atcggccatt tcacgactcc tgcgatgact tgcggataat cttctagttt gataagtttt
     5941 tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg atcatgagaa
     6001 agaatcgatc ctccaacaat tggtaagtca gcattcgaac caacaaagta atgaggcaag
     6061 acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat cgggcggtgt
     6121 tgttctgata aaatgatcga atgttcattg taataagcat aaggggaata ttggaatccc
     6181 cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc tggatagttc
     6241 aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata gtctactttt
     6301 accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga taaattgatt
     6361 gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt tgcgatggct
     6421 cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt tgcttcctct
     6481 gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga
     6541 aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga aagaacacca
     6601 ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc cttaactggt
     6661 cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg attctgtaaa
     6721 tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa
     6781 atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt ggatggctga
     6841 catgccaatc ccatgctgtt ttgataatcg ctttgatatc tgtgtattca ggtttccagc
     6901 ccagaatctc tctcgctttt tcacttgaag cgactaatcg gctaggatct ccagctcttc
     6961 ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct tctagcattt
     7021 ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca tttccttctt
     7081 tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga acataatctc
     7141 tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaag gcttttcttt
     7201 gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta tgatcttctc
     7261 cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg acatagcgca
     7321 tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta ctttcaccat
     7381 acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttc ggctctccat
     7441 aagttgctgc agtagaagag aatacgattt tattcacgtt gaactcgtgc atgacttcaa
     7501 gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt tcgacagatt
     7561 ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc ttttcaaata
     7621 cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca tctggatgta
     7681 ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac cccttttgaa
     7741 ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact aaaattgtca
     7801 taaaaaacac tcctttgatt agtaaaattg
//
LOCUS       DACCWV010000124         7765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCWV010000124 DACCWV010000000
VERSION     DACCWV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.126"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            32..349
                     /locus_tag="IVW84_002345"
     CDS             32..349
                     /locus_tag="IVW84_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7918522.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            342..950
                     /locus_tag="IVW84_002346"
     CDS             342..950
                     /locus_tag="IVW84_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7918523.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            966..1796
                     /locus_tag="IVW84_002347"
     CDS             966..1796
                     /locus_tag="IVW84_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7918524.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            1934..2422
                     /locus_tag="IVW84_002348"
     CDS             1934..2422
                     /locus_tag="IVW84_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7918525.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2473..3345
                     /locus_tag="IVW84_002349"
     CDS             2473..3345
                     /locus_tag="IVW84_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7918526.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3521..3883
                     /locus_tag="IVW84_002350"
     CDS             3521..3883
                     /locus_tag="IVW84_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7918527.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3892..4644
                     /locus_tag="IVW84_002351"
     CDS             3892..4644
                     /locus_tag="IVW84_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918528.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4658..6460
                     /locus_tag="IVW84_002352"
     CDS             4658..6460
                     /locus_tag="IVW84_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7918529.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6737..>7765
                     /locus_tag="IVW84_002353"
     CDS             6737..>7765
                     /locus_tag="IVW84_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7918530.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKIN"
BASE COUNT         2604 a         1283 c         1654 g         2224 t
ORIGIN      
        1 aatattacac ttgagaatag aaggtgacgt aatgcacatc cagattccaa ctgttcttct
       61 tgatggtact gtgctagcag cattaagcaa ggaagatatg tatgggtatg ccttaacaaa
      121 aatcgtacaa gaaagtctct ctgtcagtga atctacacta tatccggttt tgagaagatt
      181 gaaaaaagaa ggatatcttg agacctatga tgaacctttt gaaggcagaa accgtcgata
      241 ttactcgttg acaccaatgg gaagtcgtca tttagaagaa attattgatg aatggattgt
      301 ttttcgagat tctttagacc ggttattgga ggagagcaca aatgaatgaa tatttgaatg
      361 aagttgtgga tcatcttcaa gaaattcctg aagacgatcg ttttgatttg atccagtatt
      421 atgaggaata ttttttagat tctggaaaaa cattagatca gatcattgat gaatatggta
      481 caccaaagca gtttgcctta aaattaaaaa tcagttactt ttcagaagaa gaacaagcag
      541 aaaacgagtt agtgaaaaat ccaaaaagac aaattcgcct gatttggtta atcattatcg
      601 gtttatttgc ttctcctgtc ctcattccac tagcactggg gtttatattg gttattgggg
      661 gactattggt tgctttacta gcgctcattt ttagtatcta tgttttatgt atcagtatct
      721 taggtgttgg cctattcatg atcatctcag gaatcagtgt gtttggacaa tcgattctga
      781 gtggattatt ctttatcggt gtcggtctgt tcgccacagg aagcgcagta tttatttcgc
      841 cactgttgct gaaaggaaca aaagggcttt tttatgtcat gatgaacttt gttaaatgga
      901 tgggacgacg ttttattaca aaaagagatc tgcattcagc gggcgtttga aggaggacac
      961 ataaattgag attgaaacat tttttgatta ccggtattgg tctgatgctt actggtggcc
     1021 tcctagcagg tgcaacttat acattaggcg ctcaaaagag tctgacttgg gataatggtc
     1081 ctcggttgat cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca
     1141 tccatgcaga aaatcaaaca gtttcggtca tacgcggatt ggattttgag ataaaaacga
     1201 cttatgataa gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg
     1261 cagtcaaaaa atcaacaggt gcaatcatta gtttagaaga cacaagtgca caaatcacta
     1321 ttgttgtccc tgcagatcat gaattatctg aattgacaat cgaagggaat aatagtgcaa
     1381 ttgatatcaa cgaagtatca atcaaaaaag tgaatactaa gacacatgat ggctgggtat
     1441 cgttcaatga atttacagga ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat
     1501 tagatgaggc caatgtggat aacttgatcg tcacaggtga aaacagcaac ttccatctag
     1561 aagacagttt gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc
     1621 atgagatgga aaacagtggc tatactatcc aaacgtctgg tgaatcttat attgaaaaag
     1681 actaccagcc aataacaaat actttgttag aacaaggaca gaaaaagcta aagatcactg
     1741 ctaaagacac atcgatcaat attacgacgg atcaagagga caatgaaaca gattgatcct
     1801 gtactcaact ggatatttta agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt
     1861 catcgcttaa cgcacataac tatagtaaaa taagaaagaa aatatctaaa aagaaaacag
     1921 ggtgaaagag tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga
     1981 aagattacat acttatatca ctcaactaag ccaaaccttc tatgatccag acaaagaaaa
     2041 agtgatggtt aattacccga acaattcaga aggtcgccaa ttagagcaag tgtaccatga
     2101 agttttcaaa catttattga cggtgaagaa agagctggat tattattctt taccgattat
     2161 cgatacagga atattgaagt atgatgggaa gaaggaacga tttatcttta aatctgttcg
     2221 ggaagatttg cctctttcag cagggatgga tctagaggtc ttggtagaag actattttac
     2281 agaagaaaag cactgggttc gaacaaaatt ggattattta ccgcaggcag ccggagggac
     2341 acaggcaagc ggttggtaca tcactgaaga caaagagtta gaactagaag gtgtaatggc
     2401 gcgtattcga aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg ataaaggagc
     2461 gcaggaagta gcatgagaga aaaaagagca ttttatatca atggttatgc agggattgtc
     2521 ggattggtcg tattagcttt agttggattg tttttcttct atttaggtat gtggcaagcg
     2581 aaagtttggg cacttttttt aagtattttt ctttggttga tcacgttatt gttgttaagt
     2641 tcggcaactg tagtcagtcc taatcaagca aaagtaattt tgttttttgg acaatattta
     2701 ggtacgattc gagaaaatgg ctttttcctt acgatacctt tggcacaaaa aatgactgtt
     2761 tcgttgaaag ttcggaattt caatagttct gttttaaaag tcaatgattt ggatggaaac
     2821 cccatcgaaa tctcagcagt agtagtcttt aaagtcgttg atacagcaaa ggcattattc
     2881 gatgtagcgt attatcaaga ttttgtagaa atccaaagtg agacagccat tcggcatatc
     2941 gctagccaat acccgtatga tacattcaat gagaatgatt tgacgcttcg aggaaacacg
     3001 acggcagtgt ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc
     3061 gaagtaatcg aaacacgctt gaaccattta gcctatgcaa cagaaatcgc tagtgccatg
     3121 ctccaacgtc aacaagcaaa agccattttg tctgctcgac aaaccattgt tgaaggagct
     3181 gtcacaatca ctcaaatggc cttagagcaa atcgaagatg gacaagaaat caatttcacc
     3241 gatgatcgga aagtccaatt gatcaataat ttacttgtct caatcattac agacaaagga
     3301 acgcaacctg ttatcaatac aggcgacatg aaagaccgta agtgacatac tttttgtaag
     3361 ataggaagaa ggagtttagg acggattatt gtcttgaact ctttttttag ttgttttaaa
     3421 gaaaatgaca aaattgaaag aagaatgata gttgattcga aggcagaatt gttcgctttt
     3481 ttctataatc agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg
     3541 tctacttgtt ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt acgtagttat
     3601 gggtggagaa gaatactatg tacaaattac gacagatggt acaaaaacag tttcaaaatc
     3661 agactctgga caagaaacag tcaactatag atatatcctg cctggctata cgaaagaggg
     3721 taaggaaaaa gaactggact tcaagggaca attgactcga ccgcttcgta aaggagcata
     3781 tttaaaaatc acctggaaca aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa
     3841 agacgtgccg aaaacagcac tggaaaaact aattaaaggg tagggaaaga aatgaacaaa
     3901 atagtcgaag tacagaatgt aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa
     3961 gcattaagcg gcatcagctt cgatgtagaa aaaggcgaat ttattgggat catgggcgct
     4021 tctggttctg gtaagtcgac acttttgaat attttatcca cattagataa accaacagat
     4081 gggcatatcc gaatcaatca aaaagatgtg accacattaa aaggaaatca actagcagat
     4141 tttcgtgcga atgagattgg ctttattttc caagatttca atttattaga aagcttgaca
     4201 gcacaagaaa atatcgctgt tcctctttct ttacaaggcg tcagaccaaa agaaatcaag
     4261 caacgtgtcc aaaaaattgc agaacgttta tccatctctc atattttgga aagctatcct
     4321 tctgagattt ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa
     4381 ccaacgattc tattagggga tgagcctaca ggtgcgctgg attccaaaag tgcccgggat
     4441 cttttagata cgatggacga attgaataca aaagatcatg tatcgattct tcttgtgacg
     4501 catgatccgt tttctgcaag ttattgccaa cgtattttat tcatcaagga tggcggtatc
     4561 catcaagaag tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga aatactatct
     4621 attcttggaa acttggaaca gtagggggag attgactatg ctttggaaat tatcacttac
     4681 tgggatcaag agccgtttaa gagactactt ggtgttattc tcaggattag ttatggcatc
     4741 tgctattttt tacatgtttg aatcaatggc aagcaacgaa gcttttttga aaagtaatac
     4801 gatcgttgcg gctacagtta tcattttccg attcggttct gttttactag ggatcatcac
     4861 attcgtttat attttatatg caaattcctt tttgatgacg atgcggcaaa aagactatgc
     4921 aatgtttatg atgctgggag caaaaggcag aaaaatcgca cagatgattt tttctgaaac
     4981 attcttagta gggatcgcag ctactttagt gggttcagct gtcggtgtag gattgacaag
     5041 tatcgtccac cgattattag tggatcagtt agatctcaat atttcgcatt ttacaccttt
     5101 tagtattcac gggttgttga tcactgtttt gttttttgct gttttgtttt tattagctgc
     5161 tgttgtcaat gctttttcga ttgtcaaaaa accgatttta gcattgattc gtgcggatca
     5221 gacaccaaca agaatcaagc agcataaatt cttgtttttc cttgaagtag tactgggaat
     5281 tgcttttcta ggctgcggtt actatatgat gaaacacgtg gggacctatc aagtctttgg
     5341 tataggggta gccttagtta cgatcgtatt aggaacttat ttcattttcc attctgtgat
     5401 cattttcttt ttatcattac taaagaaatc agaacagatt tcattgaaaa aattgaacaa
     5461 tttcacattg tcacagttaa actttcgtat tcgtgaatat acacaaatgc tctcaatggt
     5521 cgctatgctg tttgccttgg cactgggcgc tttgacggta ggattaggtt ttaaaaatga
     5581 aattgcagac ttgacagaaa acaattctgg atatgatcta gtgatgaata atcctcgggc
     5641 agaagatcag caaaaaatca aagaactttc accaacactt tctgcttctt atactcaaaa
     5701 agaagatgcg ggaacgattt actataatgc aagtgaattt gcggctcaac cattgatcat
     5761 gataaaacat gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa
     5821 aatgaatgaa ctgactgtac aagaggaact tcgaagctat gaattgccgg aacagcaaga
     5881 aaaagaaatc aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc cacaatccac
     5941 tttacagcta gttcaggtaa aagacttcca agcaaacctg aaaccaatcg aaacacttgt
     6001 tgctcaaaat aaaatgaaca atccatcttt gcaaaaagta gagtacagca accagaaata
     6061 tgaaatgtat gaagtgtata atggtctatt ctctggtttt gaatttatgg gattcttcct
     6121 aggtttagct ttcttgacca tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag
     6181 caagagtgac attgctcgtt atgaaatgct tgaaaagata ggaacacgac gcgggttgct
     6241 taaacaatcg atccgtagag aagtaggcgt gttattctta gcaccaggaa tcttaggtgt
     6301 agtccatgtc ttattcggat tacaaatgtt tggtctattc atgcaaaatc catacaaaga
     6361 tatttgggtt ccgtttacga tattcttcgt attgtatttc atctactatg tactgacgac
     6421 atggttatat actggaatcg tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag
     6481 taataagaac agccaagcaa aaatagctcc tcatattttt cgtttggttg ggcttattac
     6541 cgcagatgca agcttttgaa caccgtttgt tcggaaaaag aggttgtgac aatatttttg
     6601 tcacaacctt tttttcacgg tcttttatta gaatatagta aattttttct cgttactcac
     6661 ggaataagct gttaagaaaa cggtttccat gtatactgga atcagtgcaa atgaaagaaa
     6721 agagggtgca aggaacatgg aaatcaatga acaaaatttt ggcgaaaact atcacttgat
     6781 tacgatcacg aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg cacgggttgt
     6841 tcattttcaa acaataatcg ataatgaaaa acgagagttg atcttaggtt ttgatgaagc
     6901 aaaagaatat ctagaaaaag acccttatat cggtgcttca atcggacgta tggctggacg
     6961 gattacgaat ggcacattca aattagatga tgaaacgttc gaagtaaaaa cagacccgga
     7021 cactggtcat tgtttacatg gtggtgcacc tagctttgaa gccaagcagt ggcattatac
     7081 agtcatcaat ggtgaaaacg aagcatcggt cattttctat cagactagcc cagatatgga
     7141 aaacggattt cctggaactt tagatgtaga agtacggtat acgctgacga acgatgatac
     7201 atggcgagtg acggtccaag ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca
     7261 tgtctatttc aatttaaccg gtgatccagc acagtcaatc gacgaacata tactttggtt
     7321 gaatagccga ttatttgcta cgctcaataa agatacaacg cctacaggag aagtagtttc
     7381 agtaagtggc acagcatttg acttccaacg tcctaaaaaa ttaggtgagg tctttgccag
     7441 tgattttcgc caatcaatga tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca
     7501 atctggttta ctaaatgctg cggaattgac aagcccagat gaaaaagtat cgatccaagt
     7561 aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat tttggagaag atggaccgga
     7621 aatgcgcggg aataaattag taaatcatgg cggtatcaca tttgagactc aagaactgcc
     7681 aggagcggag cgacttgaaa gttttggaga tgtcactctg gaacctaacc aagtacgcga
     7741 gtttgtaacg gaatacaaaa taaat
//
LOCUS       DACCWV010000125         7660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCWV010000125 DACCWV010000000
VERSION     DACCWV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.127"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            32..2359
                     /locus_tag="IVW84_002354"
     CDS             32..2359
                     /locus_tag="IVW84_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7918531.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2485..2814
                     /locus_tag="IVW84_002355"
     CDS             2485..2814
                     /locus_tag="IVW84_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7918532.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2854..3858
                     /locus_tag="IVW84_002356"
     CDS             2854..3858
                     /locus_tag="IVW84_002356"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7918533.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3855..5284
                     /locus_tag="IVW84_002357"
                     /pseudo
     CDS             3855..5284
                     /locus_tag="IVW84_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5490..6662
                     /locus_tag="IVW84_002358"
     CDS             5490..6662
                     /locus_tag="IVW84_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7918534.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6824..7279)
                     /locus_tag="IVW84_002359"
     CDS             complement(6824..7279)
                     /locus_tag="IVW84_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918535.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2584 a         1441 c         1525 g         2110 t
ORIGIN      
        1 attatttgat gattttttat tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa
       61 aatccaacgt atatgtcaaa aacattttgt ttctagactc aaagctgtat ctaaaagaaa
      121 atacttacat cccattatga tttcgatcat cttgattttt actttcttag ggagcttatc
      181 aagtatttat gtaaacgttt atacagaaac gtcaaatttt gctaaccaaa cgacagagga
      241 gacaaatagt ccaccctatt ctgaaataca gtccatagaa agcaccaatc agtctagcaa
      301 caacgagaca acctcaacca catcctcgac tcaactgatt gatccgacag aaaaagaaat
      361 cacagcagca aaagcagcag gggagaaagc ttattcagaa acaggtagaa gccagacatt
      421 tagtactgtt gcggccactg cgaatttcaa cggacatacc ataccagatg gatatacagt
      481 gatatatgcc aaagatggag tcattgttgc ggttaacgga tcgatgagtg cggataaaac
      541 tgcgttacaa ggcgtaacca aagcaatcgc ttttattgat aaccgtccag atatccataa
      601 tctgtttatt ggctccacag taaatggtac tactatcaca ttcccaaaaa gtatctatat
      661 gggggtggct accgctggaa atacgatcga ttcagttaca cccaatgcgg ataaaagtta
      721 ttcttttaca ccctcaacat ggggagggct tcctgccgga ggaggtatga gtactttttc
      781 aatcggtata cagttagcaa atgatattcc attcacgggg tactcgatca aaaatgggca
      841 gatcgttgat ggtaagggaa atgtttcaaa ttttccaacc tatcaagcag caacggttca
      901 ggtcaatgcg aatcggacag gaactttttc ttatacagtc aatggtggag cagcaaaaat
      961 cggaacgtac gggactacat tatctggaat ccatgtgggt gacaaagtgg ttatcactcc
     1021 aacagcagta acgggctatt cttttgcaca aacaaacggt tctttcacag ccctagaaag
     1081 taacgtcgat acggtgacct atacaggcaa acaaacacca atcactatta agtatcaaga
     1141 ctattttgga cagatgattg ctccttctaa aataatgaat cttacttacg gaagtgctgc
     1201 acaagacctg acgactaata cgcctactat caacggatat acctttacag ctgtttcagc
     1261 gacagaaaca aagaaccaat cagcaatgag tgttttagct agtttagata caaatggaaa
     1321 tgtaatcgta aaggacgcaa atggaaaaca gatttctgaa gtcatcctat attataagac
     1381 aaatgcaaca gtatcaatca atgcaaatgg cagtaagtac tatgatggtc tttctgttgt
     1441 accgatcgtt aaatatgcct ttaatgataa aacagaatca acatcccttt taaacaatga
     1501 tctcagtatt gatcaaatca catggaatcc aactgatttc aaagcgatga atgaaaaggg
     1561 agttgaagta actgcgccta ctgaaattgg aacctatacg tcttggcaat tgacaaaaag
     1621 cggactagct aaactagctg ccagaacaaa ctatttattt actatcgtgc agacatctga
     1681 tgtctatacg attaagcaaa tcagcggtgc ggtagagctc agtggcagta agacatatga
     1741 tggaaaagca ggtgtcccgt caatccatgt caagctagca gagggtgtca ctagttcgct
     1801 tactccagta caagtggcac ttagcagtat ggattacaca gcagatgcac aatcagcaaa
     1861 aaatatggtt aatgctggca gctatacaat taaactaacg aatagcggaa tagacaaagt
     1921 caaagcagca aatcccaagt tgtcatttac tgatatagcc aacacatcag gaacttatac
     1981 catctataaa gctaacgcag tgattactgt cgatgacgct gcctttaatt atgatgccca
     2041 aagtcactcg atttcggttg gaaatctcca cgttaccggt gttgtcttag gcgaatcatt
     2101 ggattatacc ctaacagtta acagccggac agatattgga tcacaaatag taacgattag
     2161 tttaggcgaa ggaagtgtaa acaataatta tacgattatc gcaacagata ccgctaagtt
     2221 gacgatcaat ccgactctct tgcttccttc gacaggtggg ataggtctac tgccctttgt
     2281 ctttttagga ctgatcttta ttttttctgg cttcttttat tttatccatc gtaagaaaaa
     2341 agcaggtgaa cagcgatgaa aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc
     2401 ttttttatcc tactggtctt ttttataggt tctccgactg tgtcagctag agaatcacct
     2461 attacattta ctttgcataa tcgaatgttc gaaagcgata agatgccaag tatcagtcaa
     2521 aacgacggaa taaatatccc ggataacttt caaggagtaa aagggttgaa cggcgttact
     2581 ttcgctatat atgatgtctc ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg
     2641 gaagacgcac aaagaaaact agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat
     2701 gtgacaaaaa cagtagacga ggaagagggc attgcttctt ttagtgcatc ggacaaagat
     2761 gagcaaggtc gtgacgctgt ttaccgattt gcggaaataa agacttcaga ttaagtaaaa
     2821 gaaaaatcag caccatttgt cgttgtgctt cctgtgacag attcatctaa tcataaattg
     2881 acgaccatcc acttgtatcc aaagagcgaa caaaaaatac ctgctgcact cacattaaca
     2941 aaaacagtag agaataagca gacagatttt gcagatgggg ataaagttcc ttatctcatc
     3001 accgtaacaa ttcctgagaa catcaacgag ataggaacct atacgatcaa agatacagct
     3061 gatcctcagt tatggctaga accagaaacg atcgattttt tgattggtgg agacaagatt
     3121 catacatttc acacacaaaa aacaaaacat ggttttgtct tatcagattc tggaaaagat
     3181 cttagttcgt ttgctgggaa aaaattaacg atccgttacc aaatgacatt gaaagaaaac
     3241 agtgaaaaaa cgacatttga taacgatgta cgtttgacga ctgacaatca aacagtagaa
     3301 acacatcttg ccatccaaac aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa
     3361 caaaaactag cagatgctca atttctagtc aaaaaaaatg accaatactt agcgcaagaa
     3421 aacgggataa atcattgggt agccaaagat gattcaagag caacgatttt tacttccagt
     3481 aaagatggaa cattttctgt ccatggattg tcttttggaa gttacgaact agtggagatt
     3541 aaaccgccta aaggatatat attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc
     3601 agttatgatc cgcttcatcc tataccattg gaaatcatca acgaaccaaa agcaaatccg
     3661 acgaataatg gaaaaacgcc agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat
     3721 ggaggaagct ttccaaaaac aagcgaagag atgcttggag gtttctcaat tttagggttg
     3781 ttgctagtcc tttcaacagg gacagcttgg ttttataaaa aacaacaaaa aggaaacaat
     3841 aggagggaaa taaaatgaaa aacatcaaaa caaaaatatg gacgttttta ggaactgcta
     3901 tcatgctgct tcctttcgtt ctcggcctag gaactgcaga ggtttctagg ccgtatcacc
     3961 tacaccagaa aatgttacgg ttaatttgca taaattgaaa ttcacttcag caccagaaaa
     4021 tcaaataaac aacggaacgg aattgacatt tccgaatagc gaaccgttga acggcgttga
     4081 attcaacgta tatgacatca ctgctacgta ctatccaagt aaagacacag cagttccagc
     4141 agacgctaca cccttcgcaa gtgtaacgac ttctggtgaa ggattagcta acctgactct
     4201 tccaggtaaa tctgatggga aagatgctgt atacgtcttt gtagaaacac caaagcccgg
     4261 tgtcgaaacg tcgccaaata tcgtgttaag cttacccttt tataacccga attcatcaga
     4321 ccctgacaaa cctttagaca cgattcacct atatgctaaa aatgtcatca aagaaagcaa
     4381 tatcaaaatc cagaaagctg ggaacttttc aggggtagat aagttagcag gggcgcaatt
     4441 ccagatcaaa aattcagaga atcaattcgt gaccggtttt gatgaaaaca ctggtgtcgc
     4501 tctctatgat cctgctgagt cagcgttgac attcacaact gatactgaag gatccatcag
     4561 tgtcaatgga ttaatagacg gcaattatac tttagttgaa acaaaagcac cagatggtta
     4621 tcaattaacc aatccacaaa aagaaactac ttttatagta aaaaacggaa aattgcaaca
     4681 aacaaccggt atcatccatg gcgcagatga agccaatctt gggaatccaa acggtgtagt
     4741 caatcgggaa aatacaacta cggataacac gattactgta gaaaatgtcc aaaactacgg
     4801 tgatttccac ttcatcaaac aagatgtgaa cacgcaagaa aaattagccg atgcagaatt
     4861 ttctattcta agagcaaaag atccaactgt taaattaatg aaaaaaatag caaatcttgc
     4921 ctcaggagaa tacccatatg cttggtcaga tgaagtcaca ggttctgact gggaagaagt
     4981 gaaattgatt tctgattcag aagggaaatt ttcaatcgaa gggctgaaac aaggggcata
     5041 ctgccttcaa gaaacaaaag caccagaagg ctacgcatta cctaagaatg aagctgcgta
     5101 cactgaattt acagtagatg atgatccaac aacttctgat gaaacaacga tcgataacca
     5161 acctaaagga atcttgcctt caactggtgg tatggggatc atcgtattca tcttggtagg
     5221 tactgcttta gttggaggag cagtgatcta cttcaaaaaa cgccatcaag aaacagaagc
     5281 ttaaactatt ggaaacaaaa atcatacgat aaataaacga tagtgattca aatggggaga
     5341 aaggttgtga caagtggtcg gtctcttggt attcgctcaa caaacggcaa aataggagga
     5401 actattttgc cgtttgttga gcgaatactc aggtcaaggc gctgatttca caacctcttc
     5461 atatacatat tagaaaatag gtggacatga tgaataaaag cagaataaga atactggaca
     5521 tcttcatggc aatcatttta gtcgttggga tcgggatctt ttcttatcct tttgtagaag
     5581 attcactgaa tgattttctt gcccagcaaa tgatcatcca ttatcaaaag caagcatcga
     5641 ataagaattc agaggaaata aaaaaacagc aggaaaaaat gacaaagaaa aaccaacagt
     5701 tggcagagca aaacgtatca ccagggatag gcagtttcaa ccaagcagtt gatgcaaaag
     5761 cattaaaaga tttacctagt aatgcctttt ttatggaaca tatgcttgga gtcatcgaaa
     5821 tcccaaaaat caatgtcagc ttacctatat tcgatcagac aacggaaata tttttgcaaa
     5881 aaggaacgtc attattggaa gaaagttctt atccgactgg cgggaaaaac acccatgccg
     5941 ttctttctgg gcatcgtgga ctcccagaag caaaattatt tacagatttg cctaagttga
     6001 aaaaaggaga ccagtttttc atccagatca atggaaagac attggcgtat caagtagaaa
     6061 aaatccaagt ggtcttacct gatgaagtag attctttggg tatccaaaaa ggcagagatc
     6121 tcgtgacttt gcttacctgt actccttata tggtcaatac acatcgactg cttgtgacgg
     6181 ggcatcgtat tccttatcaa gcaaaagagg caaaaaaagc gatccaagga attgatcaat
     6241 ggaaaaaatg gaaatttttt atttggttta tcggtatact acttggatca atcggcttag
     6301 tttggctact catcgcttat ttagattctt tggccatcgc taagcgaaac tatcctttat
     6361 ctttttatgt aaagaacaca aacggacgac caatcgaagg aatggtcttt tctgtaaaaa
     6421 cgctcaacgg caaacactat attactagag aaaaagttcc atttgtgaaa gcttctgatg
     6481 aatacggact agtgagattt tctgatttaa aaggcaggaa ctatcgcttg caacatgagg
     6541 agctcctgct taaaatccat gtaaaacata aacattcgaa acagttttca atgaaattga
     6601 aaaaaggaag atataaactg cgaaaagaaa aagaggtgta ctatttgatt gagaaagaat
     6661 gaatgaagat gtccaaaaaa gcagagggaa cagactaaat gaaaaaagag agaagctgac
     6721 gaaatggacg tgtaaactgc tatttttggt ttactatctg gaacgccagc ttctattttt
     6781 ttatgtttca gaagaatttt attattatag tataactttt gaattagttt ccataacatt
     6841 tggagcaagg ggttaaacca cgagcttgag cttcgcttaa agtggcaggg gtatacgtac
     6901 catttccaca tttatgtgta tggtattttg aaccggtcgg tgtgacatag acagtttgtt
     6961 cattattttg ctcaggagct gcggcttgtg cctgctgaga ggcctgttca gctgctgctt
     7021 gctgtcgcgc ctgttctgct tgttgctgtg cgattgcttc ttgttgcgct tgttctgctt
     7081 gttgtcgtgc aatttcttct tgctttttcc gttcttcttc tgcttctttt gctttgattg
     7141 tggaatcaat tgcagcaaga cggcttgtca actcttgttt gttttcaggt agcgcttgga
     7201 tagctgctga agcagttgca tagttttctt ttgtaggatt tgcttcagct tgtgacaaag
     7261 cagattccgc ttctgtcact tttttttcta gtgcagcttt ttccgctttt tgtttctctt
     7321 cttctttttt cttttcaatt tgttcttgtt ctttcgatga atctgccgta ctttcggaga
     7381 tcgtggtact gctgctggaa gcctgagctg aacttgtgct ttgagtagta gacgtagtag
     7441 caccacaccc actcaacaaa cttcctgcga ccaaaaacaa tgcaactaat tttttcacta
     7501 aatttcctcc ttaagtaaaa aacaaccgtt ttcaataaat gtccttttca attgtttact
     7561 tacttgttat aataattgtg ttatgaatag tgattgagga aacttaatct aatcccgttt
     7621 tgatacacgg gattttttgc tatagttctg ttattaagta
//
LOCUS       DACCWV010000126         7653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCWV010000126 DACCWV010000000
VERSION     DACCWV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.128"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2008)
                     /locus_tag="IVW84_002360"
     CDS             complement(<1..2008)
                     /locus_tag="IVW84_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7918536.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2024..3020)
                     /locus_tag="IVW84_002361"
                     /pseudo
     CDS             complement(2024..3020)
                     /locus_tag="IVW84_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3218..4459
                     /gene="sstT"
                     /locus_tag="IVW84_002362"
     CDS             3218..4459
                     /gene="sstT"
                     /locus_tag="IVW84_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7918537.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="IVW84_002363"
     CDS             4606..5241
                     /locus_tag="IVW84_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7918538.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="IVW84_002364"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="IVW84_002364"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7918539.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..>7653)
                     /locus_tag="IVW84_002365"
     CDS             complement(6317..>7653)
                     /locus_tag="IVW84_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918540.1"
                     /translation="ITFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRA
                     SLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTL
                     FLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPS
                     DMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPW
                     QLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHL
                     SGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEI
                     NPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTI
                     PPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2506 a         1198 c         1446 g         2503 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt aattttttta
     7081 taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta tagttgttat
     7141 agttattatt taaaatgtgt gtcagcatat cactaggagc cataccactt aattcttttc
     7201 ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg
     7261 cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt
     7321 ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga
     7381 cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag taccattttt
     7441 ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag
     7501 cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg
     7561 tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa
     7621 aataagaaac gaccattgtt aaaaaagtga taa
//
LOCUS       DACCWV010000127         7650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCWV010000127 DACCWV010000000
VERSION     DACCWV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.129"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            96..530
                     /locus_tag="IVW84_002366"
     CDS             96..530
                     /locus_tag="IVW84_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918541.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(608..1141)
                     /locus_tag="IVW84_002367"
     CDS             complement(608..1141)
                     /locus_tag="IVW84_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7918542.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1235..2413)
                     /gene="mutY"
                     /locus_tag="IVW84_002368"
     CDS             complement(1235..2413)
                     /gene="mutY"
                     /locus_tag="IVW84_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7918543.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2406..3203)
                     /gene="recX"
                     /locus_tag="IVW84_002369"
     CDS             complement(2406..3203)
                     /gene="recX"
                     /locus_tag="IVW84_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7918544.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3310..4692
                     /gene="rlmD"
                     /locus_tag="IVW84_002370"
     CDS             3310..4692
                     /gene="rlmD"
                     /locus_tag="IVW84_002370"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7918545.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4866..5444)
                     /locus_tag="IVW84_002371"
     CDS             complement(4866..5444)
                     /locus_tag="IVW84_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7918546.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5766..6236
                     /locus_tag="IVW84_002372"
     CDS             5766..6236
                     /locus_tag="IVW84_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7918547.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6344..7570)
                     /locus_tag="IVW84_002373"
     CDS             complement(6344..7570)
                     /locus_tag="IVW84_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7918548.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2225 a         1520 c         1324 g         2581 t
ORIGIN      
        1 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
       61 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
      121 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
      181 agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
      241 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
      301 aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa
      361 tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag
      421 gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc
      481 cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt
      541 cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt
      601 tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt
      661 ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta
      721 ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga
      781 catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat
      841 atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc
      901 ccttttctct gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg
      961 ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca
     1021 cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca
     1081 atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca
     1141 tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca
     1201 tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat
     1261 ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg
     1321 catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg
     1381 ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt
     1441 ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct
     1501 cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt
     1561 tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca
     1621 gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg
     1681 cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt
     1741 ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag
     1801 aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat
     1861 gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg
     1921 cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg
     1981 caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc
     2041 ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga
     2101 tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg
     2161 cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca
     2221 tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag
     2281 agatccagat acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt
     2341 cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc
     2401 attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca
     2461 aatccttttt gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt
     2521 aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga
     2581 tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc
     2641 gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt
     2701 ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg
     2761 ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat
     2821 ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc
     2881 aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga
     2941 aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt
     3001 aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg
     3061 atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca
     3121 gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta
     3181 ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca
     3241 cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg
     3301 gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag
     3361 cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca
     3421 tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca
     3481 gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct
     3541 gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat
     3601 tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct
     3661 tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa
     3721 tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac
     3781 tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta
     3841 atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca
     3901 aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa
     3961 gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc
     4021 aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct
     4081 ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc
     4141 aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc
     4201 gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg
     4261 gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta
     4321 cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta
     4381 ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg
     4441 aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac
     4501 gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat
     4561 gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta
     4621 cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga
     4681 ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca
     4741 gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac
     4801 aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt
     4861 gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata
     4921 ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg
     4981 ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca
     5041 ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta
     5101 cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg
     5161 tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca
     5221 ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat
     5281 tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg
     5341 cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct
     5401 aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca
     5461 tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt
     5521 ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag
     5581 aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc
     5641 atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca
     5701 aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta
     5761 gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat
     5821 taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac
     5881 ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat
     5941 tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc
     6001 ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta
     6061 atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag
     6121 tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg attccttatt
     6181 atttatgggt actattcgca actctattga attattcaat ttatttttta aactaagcgc
     6241 taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatttttt
     6301 gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac ccacttgttg
     6361 ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca gcgtaaggat
     6421 cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct gtttgctgat
     6481 aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa gaataatcgg
     6541 tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc gaatcatcaa
     6601 agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt gtttttatat
     6661 ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct aaaatttgat
     6721 agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg attgtttcaa
     6781 acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag tccgtcgtca
     6841 cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct aaacttaaaa
     6901 ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg atatcgcctt
     6961 ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc aatgtttgtt
     7021 cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc actttgatgc
     7081 caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca taatgatcaa
     7141 taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca ccaaatgcgt
     7201 aggcatgatt taatttatct accgttccat aacctacgat attcgtataa atatctcggt
     7261 ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc atcatcgtat
     7321 cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat agcaaaaccg
     7381 aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat attcgattag
     7441 agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg attccgtatg
     7501 ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga actttttgcc
     7561 atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat ataactgttc
     7621 aatcagcaaa ttcacttttt aatcataaga
//
LOCUS       DACCWV010000128         7626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCWV010000128 DACCWV010000000
VERSION     DACCWV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.130"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(15..707)
                     /locus_tag="IVW84_002374"
     CDS             complement(15..707)
                     /locus_tag="IVW84_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7918549.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IVW84_002375"
                     /pseudo
     CDS             792..968
                     /locus_tag="IVW84_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IVW84_002376"
     CDS             complement(1144..1713)
                     /locus_tag="IVW84_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7918550.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IVW84_002377"
     CDS             complement(1787..2110)
                     /locus_tag="IVW84_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918551.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IVW84_002378"
     CDS             complement(2264..3076)
                     /locus_tag="IVW84_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918552.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IVW84_002379"
     CDS             3430..4278
                     /locus_tag="IVW84_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7918553.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IVW84_002380"
     CDS             4423..5364
                     /locus_tag="IVW84_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7918554.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IVW84_002381"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IVW84_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1372 g         2470 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacctatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 acttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gttacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgagaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatga atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaacg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctcgaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caacct
//
LOCUS       DACCWV010000129         7534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCWV010000129 DACCWV010000000
VERSION     DACCWV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.131"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVW84_002382"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVW84_002382"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7918555.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IVW84_002383"
     CDS             complement(2032..3273)
                     /locus_tag="IVW84_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7918556.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IVW84_002384"
     CDS             complement(3291..4214)
                     /locus_tag="IVW84_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7918557.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IVW84_002385"
     CDS             complement(4435..6927)
                     /locus_tag="IVW84_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7918558.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IVW84_002386"
     CDS             complement(6934..7401)
                     /locus_tag="IVW84_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7918559.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DACCWV010000130         7474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCWV010000130 DACCWV010000000
VERSION     DACCWV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.132"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            275..1753
                     /locus_tag="IVW84_002387"
     CDS             275..1753
                     /locus_tag="IVW84_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7918560.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="IVW84_002388"
     CDS             2030..4090
                     /locus_tag="IVW84_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918561.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="IVW84_002389"
     CDS             4173..4742
                     /locus_tag="IVW84_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918562.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="IVW84_002390"
     CDS             complement(4917..5723)
                     /locus_tag="IVW84_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918563.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="IVW84_002391"
     CDS             5861..7375
                     /locus_tag="IVW84_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7918564.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2683 a         1226 c         1459 g         2106 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaag
//
LOCUS       DACCWV010000131         7283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCWV010000131 DACCWV010000000
VERSION     DACCWV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.133"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..153
                     /locus_tag="IVW84_002392"
     CDS             <1..153
                     /locus_tag="IVW84_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
                     /protein_id="HAP7918565.1"
                     /translation="YVFLTIFLYLLKCPQSLILSALRAFCFCGKPRISRSIFLYFPCQ
                     SWLKSW"
     gene            complement(160..1353)
                     /locus_tag="IVW84_002393"
     CDS             complement(160..1353)
                     /locus_tag="IVW84_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7918566.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1432..1638)
                     /locus_tag="IVW84_002394"
     CDS             complement(1432..1638)
                     /locus_tag="IVW84_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7918567.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(1631..2773)
                     /locus_tag="IVW84_002395"
     CDS             complement(1631..2773)
                     /locus_tag="IVW84_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7918568.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3285..3920
                     /locus_tag="IVW84_002396"
     CDS             3285..3920
                     /locus_tag="IVW84_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918569.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4056..4148
                     /locus_tag="IVW84_002397"
                     /pseudo
     CDS             4056..4148
                     /locus_tag="IVW84_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4262..4897
                     /locus_tag="IVW84_002398"
     CDS             4262..4897
                     /locus_tag="IVW84_002398"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7918570.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            5004..6242
                     /locus_tag="IVW84_002399"
     CDS             5004..6242
                     /locus_tag="IVW84_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7918571.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            6469..6888
                     /locus_tag="IVW84_002400"
     CDS             6469..6888
                     /locus_tag="IVW84_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7918572.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            6893..7132
                     /locus_tag="IVW84_002401"
     CDS             6893..7132
                     /locus_tag="IVW84_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7918573.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         2165 a         1280 c         1383 g         2455 t
ORIGIN      
        1 tatgtctttt tgactatctt tttatatctt ctcaaatgtc cgcaaagcct tattttatcg
       61 gctctacggg cattttgctt ttgtggtaaa cctcgcatat ctaggtctat cttcttatat
      121 tttccctgtc aatcgtggtt aaaatcgtgg taaatacttc tatgcgatta gacgctgaac
      181 ctctgatttt gcggtatcta tggacgcatg agcgtaccag ttcattgtga tactgatgtt
      241 tgaatgcccc atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccagccttgt
      301 gcagaatgta tggcgtagcg tatgcggtgt aatatgtggc aagggattgt ccttgtggtg
      361 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatgg caacttttgg
      421 attacctttc ggattgataa acacaaaacc gctgtgtccg tctacctcta tcgactttat
      481 ctttggtcgg ctgttgataa cccgttgaaa tgcctgtatc gtttctttgc ttaatggcac
      541 ttgccttatc ccgcttttcg tcttaggcgt ttcaatataa tagccctgtt ccttactctt
      601 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
      661 tccgcacagt tccgagatac gaagtcctgt ctttaacagt atcagcacat catcataatg
      721 cttgtgatac acgttgtccg tcttaataaa tgacaataag gcttgctcct gttcctctgt
      781 caatgcgact ttctctttgg tatcattttc tatgacttca cttagcttga aatcaaaagg
      841 gtttttcctc acataatcat cttgtatggc tatgtagaat gacgctttta acgagcgttt
      901 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcttt
      961 agcgtcagag ggttttatcg tatcaatgct cctagcacct aacttgtcct cttttaataa
     1021 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgtcc tctgtgcgtt
     1081 ctgtttggca tagagttggc aaagcgtcat tttcttgcct gtgctgtcga taccgtcctc
     1141 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
     1201 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttcccaatgc
     1261 gtctacatat ttatataagt attttccgtc tgttcgttgg ctctctccag tatgcaggat
     1321 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
     1381 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagattgc
     1441 gtccaattta tcaataacct tttcaaactg tcggcgttta atctgtatgc ggttgccatt
     1501 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
     1561 aatgcggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
     1621 atcctgtgta ttactcataa aacaccccct cactttcgat tacgtctgat atgtccgtac
     1681 acatctgttc cacaagcatt tcctgttttt cagaaaggtg ggtacattca tcaatcattt
     1741 tcatgtagtt tgaacctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
     1801 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacaggg ttcatttcaa
     1861 aaggtaaact gtcatgggaa atgaatatct cccacaatgg atagtcagga aattccacaa
     1921 aatcggtaat gagataaaac accagcccat gagggttata cgtcaataac agttcttcaa
     1981 ctgcctgtac cgcctttttg tcacgcagac gaatttcaaa acggttgata atatcggtat
     2041 gtttcttttt cgttgcctgt tccttttgtt tttcatacag gcagaaatat tttgtgcttg
     2101 cctttgagcc gatataaagg gtacttgcta aattacggtt ttttccgctt aattttccac
     2161 cttgtacatt ttcatagttt ttacagcgac attccgcacc accatttctg tatttttcag
     2221 atagcacagg aatatccagc agaccgcaca tatcattgat tgccaagtca aaacgtcgga
     2281 taacaccgcc acagtcaaga cagcggttta agaatgaata ccagtccctg ttctgtgcct
     2341 gtaagacata ttccatgttg cgtgagcctg ccccttttaa ttctaaaaat acgcccatgt
     2401 gttcatcatc agaagccatg accttgattt ccccgtaagc ataatgctcc ttatagccat
     2461 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
     2521 cttccaatgc gtcagtcgtt ggaaaacgga cagacagata atcaacgagc agataaaaag
     2581 gcttatcgca gatatagtaa ttcagacaac gcaggataat gttctgtgtt tccttgctgg
     2641 cgttagcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
     2701 caagttctcc ttgtgaaaga ccgctggcaa gacgtttgtt cttcatttca agtgcaaatt
     2761 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaaccacc
     2821 tttcaaaaag tgtgatatgt actatcttct taaaacctct cattactact cacaggtttg
     2881 ggggtgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
     2941 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
     3001 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
     3061 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
     3121 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
     3181 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
     3241 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
     3301 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
     3361 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
     3421 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
     3481 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
     3541 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
     3601 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
     3661 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
     3721 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
     3781 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
     3841 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
     3901 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
     3961 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
     4021 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
     4081 aggcaacgca ggtatcacag tatgaattga caccgcataa ggaaagcaac tggaagattg
     4141 tagaataaat gaaaaaggct tgcattctgc atttttataa aattgcaagt ctttatattg
     4201 acaagcataa aatactaaga tatactttaa tcagaccaac ggtttgtatt ggaggtaagt
     4261 tatggcacga aataaatatc cagaagaaac agtaaaactc attttagatg tttctacaaa
     4321 aatgttttgt gaaaaaggat atgataacac atcattgcaa gatattatca atgaaacaaa
     4381 actttctaaa ggtgctattt atcatcattt cgattcaaag gaagatattt taaaagcaat
     4441 ttttcaaaga attggaaatg aaaatgcagt aatatttgct aaaattagag ataacgatag
     4501 tttaaacggt ttagaaaagt taagagaaat atttaagaca gcggtattca attctaacca
     4561 aagcgtttta ttgacagttt ctcctcaact attaaacaat cctcgttttc ttgctatgca
     4621 aatagaacaa atgtatgaaa ttgtagtacc tgaatttgtt gaacccgttt tagaacaagg
     4681 tatcaaggat aagtctttgg agattgaaaa ttcacatgaa attgcagaag ttatcatggt
     4741 attaagtaat gtatggctca acccattagt tgaaatgacg gataaagagg gtatggaaaa
     4801 acgttgcaat acattcaatg cccttttaaa tggaataggc atagacacat tattagacga
     4861 agaaacaatt tctgcattta tacagttttg taaataactg tatttgttat ccctatacga
     4921 tagactgctc tataaaagag cagtttattt aaaccattat ataaaccgac ggtctgtttg
     4981 attaaataaa aaggagagat tttatgcaag cagaaaaaaa actttttaat cgtgatttta
     5041 tacttgttgt tataggacag ataatttctt tatttggaaa cgctatccta cgatttgcat
     5101 taccgtacta tttgttaaaa gaaactggtt catcagcttt gtttggtatt gctacagcct
     5161 gctctttttt acctatggtt attttgtcgc tggtaggtgg tattattgct gacaggacaa
     5221 ataaaagaaa cattatggtc atactagatt ttgtaacagc aatcataatt ttagtatttt
     5281 atttgtcatt gggaaaaatt cctatcatac ctttgtttat tgttgctttg atgttacttt
     5341 atggtatttc tggtgcatat caaccagcgg tacaagcaag tattcctcta ttggtatcat
     5401 cagataaact taccgtcggt aatgctataa taaatcaagt aaatacttta gcaaacttta
     5461 tcggaccagc tataagcgga gtagtttttc aattttatgg tatcaatccc attttaattg
     5521 ttagtattat atgttttact ttttcagcca ttatggaaat ttttatccat attccacacc
     5581 aaaaaagacc tcgtgaagta ggtgtaattg cagttgctaa aaacgattta aaagatagtt
     5641 ataccttttt gaaaaaggag aaacccattt ttatatccgt aatctttttg gtttgcattt
     5701 tcaatttggt tttaagtgct gttttaattg ttggtctgcc tatcttaatt tctgaagttt
     5761 taaaaatgcc agatacacta tatggttttt cacaatcagc attatcatta ggtggtttat
     5821 gtggtggtat tttaacagct cttgttgctt caaagttaaa actaaaaaac ctttatacct
     5881 tgttacttat ttgttctgtt tctgttttac ttatgggaat ttcgctattt ttagaattgc
     5941 ctgcaatagt ttcctactgg gtagttacag tgatgtgctt tgtggctatg ggtgtatcta
     6001 ctatgtttgt tgttcaaatt tacactcttg ttcagacaca aacaccaccg caattagtag
     6061 gaaagataat ggctacgctg gtagcagttg ctatgtgcgg tcaacctatt ggacaaatgg
     6121 tttacggtat tttattcgat gtatttatac aagaaacatg ggttgttatg tttgtagctt
     6181 ctattgcttc aatcatgatt tcaatatatt caaaaagaat ttttcaaaag ttagagatat
     6241 aatacaaggg gttaaatagt atgttaggct aaaaacaata tataaaagaa aatttgaaat
     6301 ctgctaaaag gatagtgtta taaaagaaag agtaatattg cagtatttaa ggctcgcaca
     6361 aatatatgtt ttgtgtgggc ttttttctta ccatttttct gtatttctaa agggtataaa
     6421 cggtattccg tttatatatt tcagcattag cagaaagggg gtgagattat gaggacatct
     6481 tctttcgagc atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac
     6541 aaagtgaaaa gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt
     6601 gaaatagccg aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt
     6661 gtatcattta aagtcttgca ctttacaatt tatgtatctg atgaaacact tggtacagtt
     6721 ttacatagac tgtcagaaaa acagcgtagt gcgattttat tacgttattt tcaaggaatg
     6781 aacgacagag aaatttcaga actgtaccat gtatcacgtt ctgctatttg cagtagaaga
     6841 actagaggac taaggaagtt aaaaatgctt ttgagcgaaa ggaagtaaag aaatgagtaa
     6901 agattatggc tatccctctt ttgaattgat ttgcaaagcg tcaagcggag atgaaatggc
     6961 agttaaagag attttgaagt tttatgacgg ttatatttct aaattatgct tgcgtccttt
     7021 ttaccactct gaaagtggta aaatcattat gcaggttgat gaagaactaa aaggagaaat
     7081 tcatacagac atgatgaaag caatattgaa atttgaaatc ggagtgaagt aatcaactgt
     7141 ataaaaaaat cgggagatac acagtttcgt gctatctccc ttagtagttt tacggtcata
     7201 tcaaggctca ttcaatcgtg gtaaattcgt ggtaagatga gtgtttttct atacttcaaa
     7261 atgcttgaat gtatagtgtt tat
//
LOCUS       DACCWV010000132         7283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCWV010000132 DACCWV010000000
VERSION     DACCWV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.134"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(170..3319)
                     /locus_tag="IVW84_002402"
     CDS             complement(170..3319)
                     /locus_tag="IVW84_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7918574.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IVW84_002403"
     CDS             complement(3333..4550)
                     /locus_tag="IVW84_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7918575.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="IVW84_002404"
     CDS             complement(4540..6135)
                     /locus_tag="IVW84_002404"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7918576.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="IVW84_002405"
     CDS             6260..7045
                     /locus_tag="IVW84_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP7918577.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DACCWV010000133         7256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCWV010000133 DACCWV010000000
VERSION     DACCWV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.135"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..411
                     /locus_tag="IVW84_002406"
     CDS             <1..411
                     /locus_tag="IVW84_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7918578.1"
                     /translation="ESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDY
                     RQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKT
                     DHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            487..705
                     /locus_tag="IVW84_002407"
     CDS             487..705
                     /locus_tag="IVW84_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918579.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            692..3370
                     /locus_tag="IVW84_002408"
     CDS             692..3370
                     /locus_tag="IVW84_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918580.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3361..3558
                     /locus_tag="IVW84_002409"
     CDS             3361..3558
                     /locus_tag="IVW84_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918581.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3562..4173
                     /gene="mobC"
                     /locus_tag="IVW84_002410"
     CDS             3562..4173
                     /gene="mobC"
                     /locus_tag="IVW84_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP7918582.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4158..5549
                     /locus_tag="IVW84_002411"
     CDS             4158..5549
                     /locus_tag="IVW84_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7918583.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5765..6079
                     /locus_tag="IVW84_002412"
     CDS             5765..6079
                     /locus_tag="IVW84_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918584.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6072..6566
                     /locus_tag="IVW84_002413"
     CDS             6072..6566
                     /locus_tag="IVW84_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918585.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6634..6948
                     /locus_tag="IVW84_002414"
     CDS             6634..6948
                     /locus_tag="IVW84_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918586.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7047..>7256
                     /locus_tag="IVW84_002415"
     CDS             7047..>7256
                     /locus_tag="IVW84_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7918587.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2774 a          984 c         1410 g         2088 t
ORIGIN      
        1 gaatcacttt caactaaaaa taataaatta gatgaagaat tgatcgaaac atttattgaa
       61 gaaaatcttc ttaaacaaat atggggagaa agtattgtga attgcttaaa actggctagc
      121 aatagtgatt atcgtcaatt cgacaactgg tacaaaaaat tcaaatatgc gattagatct
      181 gctgaaaaag aacaaaaaat tcaactgaaa attatctatg aaatttgtaa taacaagtat
      241 tttgtagatc atgttagaga acagctatca atgactttac gtgacttgat acgaagagca
      301 aaaacagatc atcgtatcaa acaaaaagac gattatatct ttgcttctct aaaaaataaa
      361 gcgttagaac taattgaatt gcaaatcagc gaaggaatag ataagcaata agaatagaat
      421 tgagtcaaat gaaatggagg ggtatctcca cttcatttga gaactttgaa tataggaggg
      481 gttattttga ttaaaaatcg tttaatagat caagtttggt gtctagatca aaatacagat
      541 agtctcgaat taatcgagac tatttgcttc aacactatag ttctcgattt aagagctgaa
      601 aatgataatt gtgtaactca tgtcataatt aatcaaaaaa tggctcagaa gctctctgag
      661 agcctcagaa aatgggctaa gggaggtaac gatgaaaata attgaaaaaa agattgccaa
      721 taaaaaaata aattggttgg ttgttaattt tcttgttctg atagctatga ttatttttac
      781 aagtaatatt gctcatgcga gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga
      841 gtttttgaat aattctcttt atagacctta cctaaaaaag tacaatggag atttccttag
      901 tcagttcggg atttggctcg gatgggctgt ggtaaaaggc atgtttactg tgacagattc
      961 gattcaaaat atgattccag atgtgctgga tttgttcaat ttcattgaaa gtacagggct
     1021 aaataatgtt tatcaatcag ccgtaaatac aattgtcatt ggattaatga tcctttcatt
     1081 gatgtttgtt ggttacaaaa tgattactgg aaaaggaaca gtggatttaa aatcggttgg
     1141 aatgaacgta gtgatgtcta tagcattaat acttttaatg ccaaccatga tttctagtgg
     1201 tattcaattt gctaaaacat tttataagga ctctactacg attacaaatt cagatgacgg
     1261 agtcgcttgg tcattgataa aacaaggagt aacagattta gcatatatta ataaaacaga
     1321 tcagtactca agcattgata aaacggagga tcgaaataat ttaacacgaa aaaattttca
     1381 acagacggat ctcacacaag tgttaacgga taaagtgatt gataaattgg aaaaagaaaa
     1441 tccaattgcg gacaatcttc gttatgaatt agttgaaaat tctaataatg aatttgttgc
     1501 tacaaagttt tctgacaact tcttatcaac attttctgat agcttgaaat caggctatta
     1561 ccgataccaa gcaaatctat gggggatttc aattgggttg acagcgttag caatcgccta
     1621 tgtgttcagc gcgtttgtga tcattacagc aatactagaa cttgcattta aacgtgtgtt
     1681 aggtgttctt gtttttgcaa cagatattga gacaggacag cgttcaaaag ttgttctatc
     1741 agatattcta caatgttact taactgttgg gtttcaagga tttgggttat caatgtttgc
     1801 tatgtttatc aattttctga actcaggaca aggtatctca acaaatattt ttataaaaac
     1861 aattgcttat atctgtgccg tttttgtttt aatcaaaggc agtggaacag tcatgagata
     1921 ctttggtgtt gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggaat
     1981 gggcgcaatg ctgtttagaa aaggatcaaa tggatttaat cgtgctaaag gaagcggaga
     2041 aggagaagat cgaaaaccag aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa
     2101 aactggaaga gctttgggat atgcccatga acgtggatta tccggtttag cttcagatgg
     2161 agctacaatg gctagtgaaa gagcaacaaa accatttaag agtatgcgtg atatagctaa
     2221 tgataccaag aataagttta aagaaggttt agatgatggt acagtgtccg ctattaataa
     2281 aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat atgctgattc
     2341 cgtgccaaca cgtctttcag atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat
     2401 tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa
     2461 tcctgcaagc gcaattcagc aaaaagtcca acaagatatt gaagaacgta aaaatgctat
     2521 gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgtcatg aagcgaaata
     2581 cacgcctgaa gcaatgaatc gcgaggaaat aatgttaagg aaacgtgtag aagggcggac
     2641 aggcgctaat attgacggaa aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa
     2701 ttcaaataca ggattagaaa aattagtcaa agaaaatcca caaaattcta caagtagtca
     2761 tggtggccga tcagtagatg tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg
     2821 acggaatgtt gatgtaagag agaatttaca aagttcttca aatgctcaag gtactaagac
     2881 aattgatgtt cgagaaaatc tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt
     2941 tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt aaatcagtgg atgtccgaga
     3001 gaatgtgcaa ggtagcagtg caagtcaacc aaaaacagta gatgtccgag aaaatgtaca
     3061 aagttctaca agtagtcaag gattcaaaac agtagatgtt cgggagaaca ttcaaaaaga
     3121 tagtggagaa atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc
     3181 gaaaaaattt gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga
     3241 taagggatca aaatcaagac gaagattcac ttatgaagac aatgagttat tcagagatac
     3301 acttaatgat cctaatccac ttttcgataa tttattaaga aataaaaggg ggggattgtc
     3361 atgcaattaa ttctaaagat tagtacgcat gatttcttag aacttgacga attatgtgaa
     3421 tcagtaaaga acgcaacaaa tgaaaaaatc acggtcacta attatattga actgatgatt
     3481 caagaagaat taaagatgaa aaatgaaata gagagcaatt tcaaatgttg ctctctagaa
     3541 aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa aaatcgcaaa
     3601 catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact gacattgatt
     3661 gcaaaacaaa ggggaatttc tagagcagac gtttgccgtg agcggttaaa gggactcaaa
     3721 tatgaaccgc cgttgttagc tgaagatgat agggtagttt taaccaaaca gttaaatcaa
     3781 attggtaaca atttgaatca agcggtaaag gcattaaata tcattgctaa atattttaga
     3841 gaagaatctg aagataaaaa agaaaagagc ctacatttta gttttgatgc tagccgttac
     3901 aacattttta aagatgaact tgcgaatagc atagcaaaaa agttgatgat tagttatcca
     3961 gatgacattt ttttggagtc caatcggagt gccccctctt tttttaaatt atcagaagta
     4021 gaaaaagaat atttcgaata ttggatagaa aagcaaacta gaccgggtac gaaagaagag
     4081 tattcccaaa aagttgatcg gaatttaatt cactcaattc agattattgc aagtttaaaa
     4141 gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag aaaaatttat
     4201 cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgtgtt gcagtctcag
     4261 gaataaattg tagtgatgat agtgaggaag cactgcaaga aatgaattta gtacgtacca
     4321 agatgaataa atctaaagat atagcacaag cgtttcatgt tattcattcg tttgataaga
     4381 gtacttctaa agagttaagt attgataaaa tgcacgaaat agctgtcgaa tttgcagaga
     4441 aagcttttcc aaattcgcaa atcattgttg caagtcacaa tgataaagat cattttcata
     4501 gtcatcttgt cattaataat attaacatgg aaacatgtaa aaggttgcgt attgacccta
     4561 aagatttaga actaatgcga aaaattaacg atcaagtcct caatgaacat ggtcttgaat
     4621 cagtgaataa tgagtactat gaaaacttag aaaagagaac caatctttat tcaaacgatc
     4681 cttctaagat gaaatatgga aaaattaatt atcaagagtt tgtgcaaaat gcgatcgaag
     4741 caacgttgga aaaatgcgaa gtgaattctt ttgaagattt ttcggatcat ttggcaaaag
     4801 aatataacat tgaagtttat caattttcaa aaacaaataa caaacttggc tatgttcttt
     4861 acaaacaagg tgtaaatttc tatgagcgta gaggtgagtt aattacttta ggaagagata
     4921 aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca agaaagttag
     4981 ggaaaagata ttcatttgaa tctgtttctg aacaacttga aaagaattta gaagaatcac
     5041 atataaaaaa tttttttaat atggaaagag agcaagttat gtcgctaatt agtggagtaa
     5101 attacaaaac tggtgagtta aatgtaccgt ttgtggaatt agattccatc gcacaaaagc
     5161 aatttagcga ccgtgaaggc gatgaaaaat tcattaaata tgatgacttc ttgcgagagg
     5221 tagacaagca ttttgtaaat acaatgtctt tagaagatga tcaagaatac acgattaaac
     5281 ttcatacgga tgtgaatcct caaactttac gtagtgcgaa aagtaaaaca atttctatta
     5341 aaaagactga tcagaaagag ttatttctac cggcaaaggg ttttgaaaat tgttctcata
     5401 caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac gatgtaaaaa
     5461 tgtatcgtgc taaaaaggat tatgaattca cacaacaaat gattgaaaat cagcaaaaaa
     5521 ggcaacaaaa ttttgaaatg gaattctgaa tgggcttgtt agtggacttt ccagtttttt
     5581 gcctatgaag agttgcttgg aaagaagcag tttttaggac atacgccagc tacccatcta
     5641 cttgccagtc aatccgcccc gctcacgcta ggctactttc tggcaagtca gatggcgggt
     5701 aaatctctga gggagcaagc tccctacaga ctccctccca aaaaaagaaa ggtggaagaa
     5761 aaaaatgaac tatccatatt ttaaagtctc ggcttcagaa gaaactaaag aaatattcaa
     5821 caatttttac aaccaaaaca aaggaatttt tggttcaaag gcaaatatgt ttcgtgttat
     5881 ggtttcaaat ttaccagtgt tagctagtcc atcaaataat aaatttaatg atcctgaaag
     5941 cattaaattt gaacaaaaaa tttcagaact agaatcaatg ataagtaatg aagtaattga
     6001 aaagctggat gacattgatc aaaagttatc gtattctcta aaaaacaaat acaaaacgga
     6061 ggagaaaaaa gatgtttaaa gtttacttcc cgtttcagtg gctcgttaaa ggaattcttt
     6121 tagtgctact aggattgatt ctttttggaa gttatctaac aattaaagcc agacattttc
     6181 cagaagaaaa gccaataaaa attgtaacaa aagatttaaa aataggtgaa acttatcagg
     6241 cagatggggt tgaactaaca tttaaaaata ttgaagtgac cgaaaaaaga aatgaatttg
     6301 cacaacatga tcgtgtatta caagtgacct atacacttat aaatgataca gaaaatccga
     6361 tacgtccaca acaatggtcg attgaaaata aggatggtga agaactttat gagtatgata
     6421 tgatattgga taatccagag tatattgaaa gtggcaaaga agaaacagta aattactatt
     6481 atgaattacc gattgaaagg caggactacc aaatcgtagt acgtagagag aatggttaca
     6541 tagttgggtt gccatttact ttataagttt tattttgtta gtagttttta aggtaatatc
     6601 agttttgtga tttttgaaaa tggaactggg agaatggctg aggaaaataa agttgttccg
     6661 tttgaaacat tagtgataaa taaggaagag tttgagcaac tttttcttga tttttttatt
     6721 caatatgaca tgatttatga ttttagttcg tcactagatt tatttgaatt agaagcagaa
     6781 ataatagaaa acagtcgaga gaagaatggt aaaatcatta tttcagtaga aaaattcaat
     6841 gaattattct cacaaacatt tgaaatagat ccagactatt atcaaccaca agaagttttt
     6901 cgagagttta gaaataagat tattggtttt agtcaaacta caaaatgaaa gcttaataaa
     6961 gcaagtgttg atagaattat tcctcaacgc ttgctttatt ttatgggcac ttcataatag
     7021 aggtatagag aagagaggta ttcattttgg atactattac tccaacaaac ttcaaagaaa
     7081 atataggtaa agtgttgcaa gaagtagttc attcaaattc tgaaatagaa attactaggg
     7141 attctaaaaa tggattgaac gatggcgtca ttatgatttc taagagaaaa tgggagttgt
     7201 tacaagaaga actttattta caacgaacgg gtacatgggc ttatgtatct gattta
//
LOCUS       DACCWV010000134         7255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCWV010000134 DACCWV010000000
VERSION     DACCWV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.136"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            99..746
                     /locus_tag="IVW84_002416"
     CDS             99..746
                     /locus_tag="IVW84_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7918588.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVW84_002417"
     CDS             1139..3784
                     /locus_tag="IVW84_002417"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7918589.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVW84_002418"
     CDS             4094..5407
                     /locus_tag="IVW84_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7918590.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVW84_002419"
     CDS             5397..6050
                     /locus_tag="IVW84_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7918591.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IVW84_002420"
     CDS             6105..6797
                     /locus_tag="IVW84_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7918592.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IVW84_002421"
     CDS             6947..7147
                     /locus_tag="IVW84_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7918593.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2396 a         1229 c         1593 g         2037 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttt
//
LOCUS       DACCWV010000135         7200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCWV010000135 DACCWV010000000
VERSION     DACCWV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.137"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1751
                     /locus_tag="IVW84_002422"
     CDS             <1..1751
                     /locus_tag="IVW84_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918594.1"
                     /translation="FLHYRTHHEKKLIIRKEELSLQVQKGLLKIKEIPERLDRFMDSK
                     SQYLTFEPGFADVVWSQDPERNMQVYNDIEEKVTSLSETGVENIDLETLISKDVDAYF
                     QTYVKELTKSSIPKDLLPDEIWRLTNQLYDVAEKRLDRIYNEKARFAFALHLQSTLDR
                     VREGHMIVHPDLNNVRKNFKREFQVAIDLSTIIEEEEQVEIPFDEIGFISMFLSINLG
                     ESEPVKENKVDVLILMHGRQTASSMLEMAQELLGTKVGKAFNMPLVMEVQTMYEEVLQ
                     YVKNYQEHLTNGLLLLTDMGSLNTFSDLIYEETGIRTKAITMTSTMIVIEAIRMASVG
                     RSLEDIYQNIQLSFESIVRDQFKAEKSETLQRKKAVIVTCFTGEGVAAKLYQRIMPVI
                     NQNKVELIQMQFLERESFKKHIDALLEEYEIKAIAGTVDVDYQNIPFFSAYDVFDDEK
                     LNILKRIADDEIPTAKIVDSLAGSLSDVFSIDELIRSLQKTVQQIQTDLSIIVEPGVD
                     TGIVIHLAFMLDGLIKQESHRQFEKLSIFSKIYRLEMDIVRANLMALERKYQVKIPED
                     DIAFLTQMFLENKIKK"
     gene            1903..2220
                     /locus_tag="IVW84_002423"
     CDS             1903..2220
                     /locus_tag="IVW84_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918595.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            2250..2570
                     /locus_tag="IVW84_002424"
     CDS             2250..2570
                     /locus_tag="IVW84_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7918596.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            2678..4138
                     /locus_tag="IVW84_002425"
     CDS             2678..4138
                     /locus_tag="IVW84_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918597.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            4206..4427
                     /locus_tag="IVW84_002426"
     CDS             4206..4427
                     /locus_tag="IVW84_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918598.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            4458..4640
                     /locus_tag="IVW84_002427"
     CDS             4458..4640
                     /locus_tag="IVW84_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7918599.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            4640..5053
                     /locus_tag="IVW84_002428"
     CDS             4640..5053
                     /locus_tag="IVW84_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7918600.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            5176..6357
                     /locus_tag="IVW84_002429"
     CDS             5176..6357
                     /locus_tag="IVW84_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918601.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            6746..6819
                     /locus_tag="IVW84_002430"
     tRNA            6746..6819
                     /locus_tag="IVW84_002430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6780..6782,aa:Arg,seq:cct)
     gene            complement(6888..>7200)
                     /locus_tag="IVW84_002431"
     CDS             complement(6888..>7200)
                     /locus_tag="IVW84_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7918602.1"
                     /translation="NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRH
                     THASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKND
                     N"
BASE COUNT         2349 a         1199 c         1492 g         2160 t
ORIGIN      
        1 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
       61 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
      121 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
      181 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
      241 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
      301 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
      361 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
      421 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
      481 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
      541 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
      601 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
      661 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
      721 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
      781 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
      841 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
      901 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
      961 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     1021 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     1081 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     1141 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     1201 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     1261 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     1321 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     1381 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     1441 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     1501 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     1561 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     1621 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     1681 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     1741 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     1801 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     1861 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     1921 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     1981 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     2041 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     2101 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     2161 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     2221 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     2281 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     2341 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     2401 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     2461 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     2521 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     2581 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     2641 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     2701 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     2761 gcgtgatgct tttatcggca tactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     2821 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     2881 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     2941 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     3001 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     3061 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     3121 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     3181 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     3241 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     3301 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     3361 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     3421 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag ggttatctca
     3481 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     3541 tcatggatca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     3601 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     3661 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     3721 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     3781 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     3841 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     3901 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     3961 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     4021 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     4081 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     4141 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     4201 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     4261 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     4321 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     4381 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     4441 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     4501 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     4561 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     4621 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     4681 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     4741 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     4801 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     4861 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     4921 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     4981 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     5041 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     5101 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     5161 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     5221 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     5281 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     5341 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     5401 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
     5461 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
     5521 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
     5581 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
     5641 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
     5701 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
     5761 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
     5821 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
     5881 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
     5941 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
     6001 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
     6061 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
     6121 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
     6181 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
     6241 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
     6301 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
     6361 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
     6421 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
     6481 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
     6541 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
     6601 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
     6661 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
     6721 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
     6781 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
     6841 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
     6901 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
     6961 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
     7021 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
     7081 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
     7141 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
//
LOCUS       DACCWV010000136         6947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCWV010000136 DACCWV010000000
VERSION     DACCWV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.138"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(58..543)
                     /locus_tag="IVW84_002432"
     CDS             complement(58..543)
                     /locus_tag="IVW84_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7918603.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(696..893)
                     /locus_tag="IVW84_002433"
     CDS             complement(696..893)
                     /locus_tag="IVW84_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918604.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(988..2748)
                     /gene="efrB"
                     /locus_tag="IVW84_002434"
     CDS             complement(988..2748)
                     /gene="efrB"
                     /locus_tag="IVW84_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7918605.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(2745..4475)
                     /gene="efrA"
                     /locus_tag="IVW84_002435"
     CDS             complement(2745..4475)
                     /gene="efrA"
                     /locus_tag="IVW84_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7918606.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4868..5080
                     /locus_tag="IVW84_002436"
     CDS             4868..5080
                     /locus_tag="IVW84_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7918607.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            5082..6761
                     /locus_tag="IVW84_002437"
     CDS             5082..6761
                     /locus_tag="IVW84_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7918608.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         2124 a         1472 c         1224 g         2127 t
ORIGIN      
        1 aaaaaaggat ggtcaactca aactgaccat ccttttttat tttatggatg ctactgccta
       61 tctttgctcc ttgaattttc cttgcgcatg catccgacct atctcgacat aatgctggat
      121 gttttctttg tttttttgta tttcttcttt tgtcaaagga cggatcactt tagctgggcg
      181 accaaaagcc aagacattag gaggaataac ttttccttct gttactaatg atccagcacc
      241 aatcaaacta ttctcaccaa taaccgcatg attcaatatc gtggtcccca tcccgatcag
      301 cgcccctttt tcgattgtac agccatgtag catgcactga tgtcctaccg tcacattctc
      361 cgcaatatct acaggtgcat catgatcgac atggatgatt gtaccatctt ggatattcgt
      421 tctttgtcct atttttatcc aattactgtc cccgcgtaaa acagcttgat accagatcgt
      481 cacatcttct gacaatgtca catcaccaat cactgtggca tttgatgcta taaaccgttc
      541 catatttatt tttctccttt cattttttgt agaaactaat cctattgaat aaaggtatca
      601 tagcatatat ttatttcata agaaaaaaaa caggtaaaac ctgctcaggc cagatgtcta
      661 tttacagtat ctggtaactg acgttttacc tgtttttatt gtacatctat attttcttct
      721 attacttttt ttaaaattaa ttcgtacact tctatttgtg ctgccaagcg ttcgatttga
      781 aactcatttt caaaaaaatc agctgaatca aaaggcttcg tctgacagac gtgcgttaaa
      841 cgatcaactt ctcttttggc ttcagtaatt ttttcattta aaaactgttc catctccgtg
      901 tcctcacttt tcttgataac tttactttac cattatcaag aatagagaac aaaaaataag
      961 ttttaaactg ttttattctt atatgactta ttctgcttcc tcagcaaatt ggctgttata
     1021 cagtttttca taaaatccac cttgtgccaa taaactctca tgcgttcctt tttcaatgat
     1081 ctgaccttga tccatcacta gaatcagatc tgcatcacgg attgtcgata aacggtgagc
     1141 gatgacgaaa cttgtccgtc cctccatcac gcgatccatt gctttttgaa tcaacgcttc
     1201 taaacgggtg tctactgaac ttgttgcttc atctaaaatc aagattttag gatcggagac
     1261 cactgctcgg gcaatcgtca gcaactgttt ttgtcctaaa gacacattgt ctccctcaga
     1321 attgatggcc atatcatatc catctggcat cgttcggata aaatgatcta cgtttgctgt
     1381 tttcgcagca tcaactactt cataatctgt cgcatcaagc tttccgaagc ggatattatc
     1441 tgcaatcgtt ccttgataaa gccatgcatc ttgcaagacc ataccaaata gcgaacgcac
     1501 atcactgcga ctcattgatt tggtgtcgat tccatcaatc cttattgctc ctttgtctac
     1561 gtcataaaaa cgcatcaata gattgatcaa tgtcgtcttg ccggcaccgg tcggaccgac
     1621 cacagctacg gtttgaccag ctttcacttc aaaattcaaa tcttggatta atggtttctt
     1681 cggatcatat gcaaaatcga catgatcgaa tgtcacatct cctaaaatcg tttctggcag
     1741 tgaagcatct actttgtttt ctttttcttc tggctcatct aaaatctcga atacacgttt
     1801 cgttgccgct gatgcacttt gcaaaactga agacaattgt gtcacgttcc ccattggctg
     1861 actgatttgc caaatatatt ggatgaatgc ttgtagttga ccgacaacga tcgccccagt
     1921 aataacataa aagcttccta gaacagccat accaatataa gtagcatagg cagttaattg
     1981 aactagaggc atcatcaagc cagaaatgaa tgctgcacga aacccaaagt atttcaagct
     2041 gtgattaact tttttgaatc cttccaatgt ttctttttcg cgtccatata ctttcaatac
     2101 actaaaacct gtcatatttt cttggacgta gccgtttagc tcaccaagag aattctgcat
     2161 gctttgaaaa tatttctgcg aagcattgat cactcgtttt gagatccaga tcgatgttgg
     2221 aatcatgatc atagagatca gcgccatcat cggctggata taaaacatca ttgctgcagc
     2281 caacgtaatt cccaatatcg catttacgat accaataaaa gcttgttgta aagcaccgct
     2341 gactgcatct acatcattcg tcacacgaga caaaatattt ccttgctggt ttttatcgaa
     2401 atatgaaacc ggaagacgat tgattttttc ttctatgtct cttctcaaat catgcatggc
     2461 actttgaacg acatttgtca tcaaaacacc agaaagtaat tgcgaaatac aataccctat
     2521 tccaacaact gtgatccaaa tgagacattt gaatatataa gaaaaattga ttgattcgcc
     2581 attggcaata ttacggctga tttctgtagt aggaagtcct gtaacatatg gcaaagccac
     2641 gttgaaagcc actgtcagaa ctgtaaatag gatcactaaa agaaaagtct cacgatacgg
     2701 tctgatatat cgagctaaac gttttaagga agaaaaggtt tttttcatgc taattcctcc
     2761 tttgataatt gagaagcggc aatatcataa taaatcgggc atttttccaa caactcccga
     2821 tgcgtcccca taccgacgac ttctccttca tttaatacga tgatcttatc tgcatgcatg
     2881 atcgttccca cacgttgtgc tacgatcaat acagtagatt ctgtcgtttc ttttttcaga
     2941 cgagcacgta acttcgcatc cgtttgataa tctaatgcag aaaagctgtc atcaaaaata
     3001 tagatttccg gacggcgtat gatggctctg gctattgcca atcgttgttt ttgtccacct
     3061 gaaaaattgg aaccaccttc tgataaatgt tcattaaatc catctggttt tcgagaaatg
     3121 aattctgaag cttgagcaat ctcagctgca cgttccattt cttcttgcgt agcatcttct
     3181 ttcccgtatc gcaaattatc tgcaatcgtt ccagtaaata gcagcgcttt ttgcggaata
     3241 aacccaatct tatctcgcaa cttgcttagc cgataatctc tcacgtctac tccatcgatc
     3301 aagatctttc cttcactcac atcataaaag cgaggaatca attggatcaa cgtagatttt
     3361 ccgctaccgg tacttccaat aaaagcaacg gtctctcctg gagaggctgt aaaactgaca
     3421 tttcgtatca ctggctcttg cgaatggcct ggataagcaa atgttacatt ttgaaattcg
     3481 atataccctt tagtttttgt atcagatata ccatttggat cttctacaat cgaaggtttg
     3541 gcatctaggg cttcttggat acgtccagct gaaacagctg ctcttggata catcatgaac
     3601 acactagcaa ataacatgaa tgagaacaag gcatggaata tatactcgat aaacgcaatc
     3661 aaatctccta cacgaagctc gcctgcatcg atttgcactg ccccgctcca gatgatcaat
     3721 accattacga tattaaataa gaaataaaaa cccggttgtg caacagccat caaacggaat
     3781 aagctttttg aactagaagc ataattctca ttgactacag caaaacgttt ttcttcaaac
     3841 ttttcattga cgaatgcacg aatcacacgc aatcctgaaa gattttcccg caagatactg
     3901 ttgattttat ctaagttttt ttgttgcttt ttagataacg gttcagacac cttagcaatc
     3961 gctacgactg ccaataatag taaaggtaat gcacctacta catacaaact gagtgtcaca
     4021 cttgtacgca tgaccatgta caaactgaca atgaacatca ttggtgccat aaaaccgata
     4081 cgtaaaatat tttgcaggaa caacatgatc tgataagcat cgtttgttgt acgagtgatc
     4141 aatgaagata cgcctagatt ttcgtattcc tgatgcgaat aactttggat cttttcgaac
     4201 aaatcatctc gaatatctgc tacgatatta gtcgtaatcc tcgaaccaaa ataacctaac
     4261 aagatattca aaataatacc gattacagta ataacgatca taaatatccc ttgttgcttc
     4321 acataatcaa aatcatcatt gcggattcct acatcgatca tacgtgctaa aatcgtagga
     4381 agtcctaatt caatcaaaat aaaaccaaat acgcatatga aattgaaaaa caagaatttc
     4441 ttataacgca tggtataatg ccacattagt ttcactttct catctccttt taatcttttg
     4501 ttacattttc attactaaaa atgatagcac aacgagccta ttaatagaac acttctctca
     4561 taaaaaagtc attatgatta tagcgggatg ataaacattg attttttctt tcaatctagt
     4621 tgatttctat acttatttgc tgttttatgc tagaataaca attaattgtt gtatattttt
     4681 tatacagaag gtacggttga aaaaatatcc gaacagataa gcaaaaacga ggcttgtctc
     4741 tttgactcga atccagtatt ctagtcaaag catatgaaaa accaggtggc ttgtcccctc
     4801 gttttgtgtt gtctaaaaaa atcaactagc taaatcactg ctttacgcag ataaaggaga
     4861 caaaacaatg atctataaag tttattatca agaaacaaaa gtacgcaacc ctaaacggga
     4921 agaaacaaaa tctctttaca ttgaggcaaa aagcgatgta gacgctcgcc aacgtgtgga
     4981 agaaaatacc ccatacaata ttgaattcgt tcaacttcta gaaggaagtc atctagagta
     5041 cgaaaaagaa aatgctgatt tctcattagt ggagttttaa tatgaaactt tccatcaaaa
     5101 ataacgagac aggcgtcttt gctattggtg gattaggtga aatcgggaaa aatacgtatg
     5161 gggtacaatt ccaagatgaa atcattttga ttgatgccgg gatcaaattc ccggaagacg
     5221 atttacttgg aattgactat gtcattcccg actacagcta catcatacaa aacttacaca
     5281 aagtaaaagc cttggtgatt actcacgggc acgaagacca catcggcgga ataccctact
     5341 tgctgaaaca agcaaatatt cctatctatg ctggtcctct cgcattagcg ttgatcacaa
     5401 acaaattaga tgagcatggc ttgcttcgag atgcagagct ccatgagatc aatgaagata
     5461 cgatcatccg ttttagaaag acttctgtca gctttttcag aacgacccat agtatcccag
     5521 atgcattagg gatcgtggtg aaaacacctt ctggaaatat cgtagcgact ggagacttca
     5581 aattcgattt cactcctgtt ggcgaaccag caaacttgca taaaatggct cgtatcgggg
     5641 aagaaggagt cttgtgcctt ttatctgata gtacaaatgc tgagattcca acatttacta
     5701 aatcagaaaa aacgattggg cattctattt tgaagatctt ttcaaaaatc gaaggacgta
     5761 tcatttttgc cagctttgct tctaacatct tccgtttgca gcaagcagca gatgccgctg
     5821 tggcaactgg tcgtaaaatc gctgttttcg gacgaagcat ggaaaatgcc atcgtcaacg
     5881 gtcaacgttt aggatatatc aaagttccag acggcacatt tgtagatgca cacgaaatca
     5941 atcgcttgcc tgccaatgaa acgatgatcc tatgtacagg ttctcaagga gaagcgatgg
     6001 ccgcgttgag ccgcatcgcg aatggtactc accgtcaaat ccaaatccag ccagacgata
     6061 cagtgatctt ttcaagttca cctattcctg gtaatacaac aagtgtcaat catttgatca
     6121 atttgctgtc tgaagctggt gcagaagtga ttcacggaaa aatcaataac atccatactt
     6181 ccggtcacgg tggacaagaa gaacaaaaat taatgcttcg tttgatgaag ccaaaatatt
     6241 ttattcctgt ccacggagaa ttccgcatgt tgaaagtaca tgcttctcta gcacaagatg
     6301 taggtgttcc tgcagaaaac tgcttcatca tggaaaatgg cgatgtttta gcgttaacta
     6361 gcgattctgc gcgtcctgct gggcattttg tggctggaga tgtgtatatc gatggtagcg
     6421 ggatcggtga tattggtaat gtcgttttac gtgaccgtca cctgctttca gaagaaggtc
     6481 ttgtattagc tgtcgcgaca gtggatatca agaaaaaaga aatcttggct ggtccagata
     6541 ttctctctcg tggatttgtc tacatgcgag aatcaggaga aatgatcaat gaagctcagc
     6601 gaatcttatt ccacgcttta agagaatgtt tgaatggacc tgactgttca gaatataaat
     6661 tgaaagaagc gatgactggc gcgcttcagc ctttcttatt cgaacgtaca gaaagacatc
     6721 caatgatttt gccaatggta atgacaccag atgaagaata aataagaggt tatgaaatca
     6781 gcaccttgtc ctgaatataa gagaaaacaa atgaaaaaca tgagaagcca tttttgtttg
     6841 ttcttattcc ttgatacaag gtggctcttt catagcctct taaatttatt tactttttca
     6901 tacaaaaaaa tacggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DACCWV010000137         6720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCWV010000137 DACCWV010000000
VERSION     DACCWV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.139"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            168..1040
                     /locus_tag="IVW84_002438"
     CDS             168..1040
                     /locus_tag="IVW84_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918609.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1037..2995
                     /locus_tag="IVW84_002439"
     CDS             1037..2995
                     /locus_tag="IVW84_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918610.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3246..4097)
                     /locus_tag="IVW84_002440"
     CDS             complement(3246..4097)
                     /locus_tag="IVW84_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7918611.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4220..4687
                     /locus_tag="IVW84_002441"
     CDS             4220..4687
                     /locus_tag="IVW84_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7918612.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(4950..5432)
                     /locus_tag="IVW84_002442"
     CDS             complement(4950..5432)
                     /locus_tag="IVW84_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7918613.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5532..6395)
                     /locus_tag="IVW84_002443"
     CDS             complement(5532..6395)
                     /locus_tag="IVW84_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7918614.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1995 a         1392 c         1233 g         2100 t
ORIGIN      
        1 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
       61 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
      121 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
      181 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
      241 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
      301 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
      361 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
      421 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
      481 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
      541 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
      601 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
      661 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
      721 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
      781 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
      841 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
      901 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
      961 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
     1021 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
     1081 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
     1141 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
     1201 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
     1261 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
     1321 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
     1381 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
     1441 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
     1501 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
     1561 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
     1621 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
     1681 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
     1741 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
     1801 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
     1861 gatgctagca gcagcttttg cttcattctt tactggtgtg acagaaccac ttgaattttc
     1921 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
     1981 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
     2041 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
     2101 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
     2161 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
     2221 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
     2281 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
     2341 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
     2401 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
     2461 acatggtggt gctgcagcac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
     2521 tgccggtgaa acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
     2581 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
     2641 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
     2701 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
     2761 caacactgtt gacttaaaag gcgaaccatt cactttatat gtagaagaag gtcaaaaagt
     2821 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
     2881 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
     2941 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
     3001 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
     3061 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
     3121 taaacgaaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
     3181 agctttttca caaaattttt cttcatactc aaaactaatc ggctttttca caacctcttt
     3241 atacattatt cctgatccaa tatttctttt aatgctgctt caagagaagt taatggtttt
     3301 cccaatactt tttctaaatc atcagaggaa atagctaact gatccttttt aatatcatgt
     3361 tggatcgcta aaaacatgtc agctgcttct tgagggaatc cggcttcttt tagattttcg
     3421 acaaacgctg catcacttgc ttcaataatt tctaactctt tacctgtcgc acgctctaat
     3481 gccttggcta attcttcata gtcagttaga ttgccggata attctaagat ttctggagag
     3541 tcactttcta ctagtacttt tgctgcagct tctgcatatt cacgtttcaa tgcccaacct
     3601 gtttttccat ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg cccaatgata
     3661 ggtaattcat tctctacata ccaattattc cgtaaaaatg tatgggaaat acctgaatcc
     3721 ttaatcattt tttcagtgaa ttggtgatct ggcgctaata cactgatcga gtgatcggca
     3781 tctgcaaaac ttgtataagc gatataagat accccagctg cttgtgctgc cttcactaca
     3841 ttcttatgtt cggcttgacg atttcctgga acgccagaga caaaaagcaa tcgatctatt
     3901 cctttcaacg cttctttcat tgagtctaaa tcatcataat caccaatacg tatattaaat
     3961 ccagcttctt tcagtgcttc tcctttttct tcactacgta ctagtacata gatttcattt
     4021 tgtggaacca tcttttttaa gaattccagc gcataaccac caaaacctcc agttgctcct
     4081 gttactaaat aatgcatatt tgccactccc tattcttttt atttgttaag atttacagct
     4141 gtacatttat aaattacacc tttatcataa agagtgtggt aagataagtc aagcaaaaaa
     4201 aatgaaagga tgacaactga tgaagtattc tctgcaattt agtgatgcaa tccatgtact
     4261 tgcctatatt gaaatgtttc aagatacccg atcactttct agtgagatga ttgccagtag
     4321 tatcgaaaca aatgcagcaa atgtacgtaa aatcatgagt cagctcaaaa aatccggttt
     4381 gatctttaca aaaaccggaa aaccgcagcc ttcccttgcc aaaagtccag atcagatttc
     4441 tttactagat gtctatcgca gcatcgaagg taatacacag cttatccaag tcgatccaaa
     4501 aacaaatcca aattgcgtcg ttggtgcgaa tatccaagaa gctttagaag aaagctacgc
     4561 tcggctgcag aaaaaggcag aagaagaaat ggctaagatc acacttgata gtctgataca
     4621 taaaatagcc gttttggaaa aagaaaaaag accagaaaac acaggcctcg tagagagatt
     4681 tttataattc tctctactgt gcctgcattt ttctgatcgg aatcgtctac ataaattctt
     4741 ttattctccg ggggacaaaa gccttgtcat gccccttttt cctctctaat gctcaggtca
     4801 aagcgctgaa gtcatgacgt cttgataaac ggtgttcaag tagctgaccc tcggcattag
     4861 ttctgaaaaa tgcaaaatat ggaaagctat ttcccatttt tcctctctaa tgctcaggtc
     4921 aaagcgctac tttcacgacc tctatattat catggtttat tgactaccgg tatccagatt
     4981 tctcccatat atttcccgtt ggtaactccc atttcgacta cagcatttgg acccccaaca
     5041 taggcgtaat cttctacttc tcttaaaaca tgcccaaagg ccgtattcgt caaatcattt
     5101 cttaattctt caggcgattc tgctaaagcg cttacaacaa gatacgttcc ttttgggaat
     5161 gggatgattc gcgtagtgtt tggaacatcc tgactcgttt gaacaccgac atagtacatc
     5221 attttcccgt tatagggttc gttgactgta aagaaataat gattttcagc agcttgtttc
     5281 aatgcatcta ttgttccatt accgataact ttttgctcaa atgcttgttt ctctgcagcc
     5341 aaaccgacaa aatccgtatt gctactcttc aattccacac caatacccgt caccgtaaaa
     5401 tctggttttt cttcgatcct atactttgcc atttctccca ccctttctat tatattaagt
     5461 tcatcttaca taaaattaaa gggaaatgac acgattacgc tcttttttct agggatattt
     5521 ttatttttta actatttttt tgctttcact gcaatccaaa tctcactgta aacattactg
     5581 aaatcctctc cccattttgt aaaagagatc tcgggagctt cgactaaccc ataatctgaa
     5641 gaaggaagcc attctgcata gattttcccc caagtatctt gtaaagtttg gggaaatggc
     5701 cctttattag gaaaaatggc ccaagtgtgt tcttctatag caagcatttc taaatcagcg
     5761 tacggatttt cttttgtcgt agcaaagcca atcatgtgca tcaaactgcc cttttcttcc
     5821 aagcggtctc catcaaatcc ataagaaacg ttgattactt gatttggata taaatctcct
     5881 aacttatgca tctcttccct ttgagtctcc gtgatcgtct gcgctaactt ttgtatttct
     5941 tgattgactc cttcaaattt gacaggaact tttttcgcca cacctgccac ataaaactgt
     6001 tctttctttt cgatacgaaa ttccatcgat tttcctcctc tgacatcaat aaaaaatgtc
     6061 aagcgtggaa aagatttttg tatgccgtat ctgctgattt ctgaaggcag atatcctgac
     6121 caatcacgaa aagctcttga aaaaccttcg actgtttcat atccatatct aaacgccgtt
     6181 tctgtcactc ttgatccatc tagaagatcg cgattagcca aagccaatct acggtttttg
     6241 atatagtcca ttagcgacat ccctgcaata aacgtaaaga tgcgttttaa ttgatattca
     6301 gagattcctg tattttcggc tattatttta taggaaagtc cttctgtcaa atgttcttcg
     6361 atgtaatcga tcaattggtt cagatcttta agcatattta tcttcctcct tgattgcctt
     6421 tttatcatat tatcaaagaa agtcaattac ttgatgaatc aggaaccaat ttagtcagat
     6481 gtagaaaatg ttgtatggat ctaaagaaaa ttctcaagca gcgtttcgta acggataatt
     6541 ggtattatct gcctatctat gttttacctt tagaaaaata aattttgggg aagacagatt
     6601 ctaagtaaac aaagcggtaa aaatatcttt tattccctta gttaatccgc ttactaccta
     6661 ctgtacaaag caaattgttg ttcaaactgc tgatcagaac gctgcttgga tttttctttg
//
LOCUS       DACCWV010000138         6608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCWV010000138 DACCWV010000000
VERSION     DACCWV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.140"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..784
                     /locus_tag="IVW84_002444"
     CDS             <1..784
                     /locus_tag="IVW84_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7918615.1"
                     /translation="IELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTW
                     ISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVN
                     LFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKN
                     IVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHL
                     HLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            774..1514
                     /locus_tag="IVW84_002445"
     CDS             774..1514
                     /locus_tag="IVW84_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7918616.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1511..4273
                     /locus_tag="IVW84_002446"
     CDS             1511..4273
                     /locus_tag="IVW84_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7918617.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4286..5194
                     /locus_tag="IVW84_002447"
     CDS             4286..5194
                     /locus_tag="IVW84_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP7918618.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5194..5814
                     /locus_tag="IVW84_002448"
     CDS             5194..5814
                     /locus_tag="IVW84_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918619.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5818..6267
                     /locus_tag="IVW84_002449"
     CDS             5818..6267
                     /locus_tag="IVW84_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918620.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            6267..>6608
                     /locus_tag="IVW84_002450"
     CDS             6267..>6608
                     /locus_tag="IVW84_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918621.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYR"
BASE COUNT         2372 a          897 c         1406 g         1933 t
ORIGIN      
        1 gattgaattg aagttttcta ttattcaaac ttggatcgat ttaaaatata atgccattac
       61 tacttggaat aatattaaac agtttttcaa agatacttgg aaaaatatta aagatacggc
      121 atacaatacg tggatttcta taaaaaatac catgattaat acatggaata acattaagga
      181 ctctttctgg aatattgtta ctggaattgt taattctgct gaaaatgcat ggacaaatct
      241 aaaaaatggt gtttcaaatg ctattaatcg ggtaaaagaa atctttaatt cgttaaggga
      301 agtcaattta tttgaaattg gcaagaacat cattgatgga cttataaatg gtgtaaaaga
      361 aaagtggaat gctttgaaag atactatcaa aggtattgca ggtaatatca aaaattggat
      421 taaaggagct ttagatattc attctccatc aagatggatg cgtgatatgg ttggtaaaaa
      481 tattgttcaa ggtattatcg ttggtattga taaagagcaa agtaagttag atcaaaccat
      541 gacaaattta gtaaaaacac cgtctgttca acctgtagta aatggaataa gtcacctacc
      601 aattacacaa acaaagcaaa atatatctgc tagtgaaaca aaagaaattc atttacattt
      661 aaatgtttat ggagatttac ctgattcaat ggttcggaag attaccacaa aaattaaaac
      721 agaattgaca agacaaatga aacgagatgc tggtgcagta ggaggaacat tatatgcaac
      781 ttaagagagg acaatttttt attaatcaac attattcttc tgaatttaat gtgtatattc
      841 aaaacaggcc tgcttctgtt tcagctagtc gagtaattga attgagagag cgagaaggaa
      901 atgattctat cattattgat aaaggctatt acaaaaatgt gactagaaag attgagtgtt
      961 actataaagc accttcaatt gatatggtgc aggagtggga agatcgaatc actgaatggt
     1021 tagatatgtc gtcttatagt gattttattc tttactatga tgaacaatat atttatcaag
     1081 ctgtagtaat agaaggacct gaatttaaag gaacaagaaa aacaggaaat attgtccctt
     1141 ttgaatttac agtgagtatt cgaccattta aggaaaatta caatggtaga tttactattc
     1201 gacaaacaga aacttttgaa atttataacc cagagaagta ttcttcaaaa ccgcttatta
     1261 aattgagtgg ttctggagat gcttcttttt atattaataa ggataaatat gatttgaaat
     1321 cgttggacag agaattattt atagattcta aattagaaga ggcttatcga aaattagatg
     1381 gtaatttaga acatcaggat caggtcacct tatttttaga ttttccattt ttatatcctg
     1441 gaaaaaatga aattaaatgg acgaaaaata ttcattcatt tgaaataatg cctaggtggt
     1501 ggagaaaagt atgaaaccaa gaatatatag tcctactgaa acagatttct caacgaatgg
     1561 cttaggaatt ttaaaggatt gtacaagatg tggaatatat gaagtagcta atggaaaata
     1621 tgaattagag ttggattatc ctttaggaac tagatttgat gaatattttg aaaatgacta
     1681 tcaaataaaa gcaaagccaa acgatcaaga agagtatcat atctttttta ttgatgataa
     1741 agatatagat acttttttaa atacagtaac tatttatgct cagagtcgta caaatcgact
     1801 tggaagacgg gtagtcactc ttgtggaagt ggactctaaa actggtcaag aagcgatatc
     1861 aattattgaa actaaaatgg ataaaaaatc tgacatacga ctttattctg atattacggc
     1921 tgtttctagc acaatctttg aagcgagaaa tgttttaaat tgtattgctg gtgaacaagg
     1981 atcattgctt caatattggg gcggagaaat taaacgtgaa ccatttaagc tttctttgtt
     2041 aaagcgaaga ggtcgcaata atgttggaac gattcggtat ggaaaagata tgtctggttt
     2101 gaaggtcaaa ttagattgga caggtataaa aacaagaatt attccatatg ctgatcctca
     2161 aagtgaagta ggtacgacta atcgaattta tggttcgcca gtagatagtg cctatattaa
     2221 taactatcct gatgtctata cagaacatgt tcagtttaca gaagaacaag gagtaaaaga
     2281 cattaagagc ttaaataaaa tagctaaaaa ttactttaaa acgatcaatc ctggctgtga
     2341 taagcctaaa gtttctatta cggttgaatt tgataagttg actgatagtg aagaagcgaa
     2401 agaatttgcg aagattagaa attatggtct gtttgataca tttaaaatat atcataaaaa
     2461 atataatatt tatcttgaat caaaggttag tggactacaa tacgattctt tatctgaaaa
     2521 aactttgaaa ttagaagcag gagacattca agttgctttt tatcagcaac aagctgtaac
     2581 tattcaggat aaattaaaag attatgcaac gaataattat atgagtgatt ttaatgatta
     2641 tgtttcttca atgattacag gtcaaggcag tgcaggagga tatgtagttt tatggccaaa
     2701 agaaaaacct tccaacatct ttattatgga taatccagat ttagaaaagg caagagaagt
     2761 actaagaatg aataaaaatg ggattgcttt ttctaagaaa ggatggcggg ggccttttaa
     2821 ttcagcttgg acattagata gtatatttaa tgccaatttt atacaaactg gattaatcaa
     2881 agcagatatc tttcaaaatt cttttaacaa gactggagat gtattgaaat tagttaatgg
     2941 actactccaa atttggaaca acaaaaagaa aataatggag ttaactaaaa aaggaatgga
     3001 attttggaat tctaagggtt caattggaac gattggaaca actgattctg ttggtaatcc
     3061 ttttcctggg gcttctactc caacccctat tgaagataat tctttagtta ttcgtacaaa
     3121 tggagacggc aaatatattt tgatttctcc taaagtagga aaaggattag ttttattagg
     3181 aaatggtaaa gcaatttatt ttggagactt agatgtacaa ggaaaactta cagttaaagg
     3241 aaaagaaata attcctggac aaggtggtgg tccatcggga ggtggagaat ctactggtgg
     3301 gtatcctagt gaattaaaaa cagatgcaga aaagagagct tggagaattt acgatatttt
     3361 gtgtaataac ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga
     3421 aacaggagga acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt
     3481 tcagtgggat ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta
     3541 tgttcaaaac ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca
     3601 agttcgattg cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac
     3661 gacggttgat ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg
     3721 aaactatgaa cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta
     3781 taacaaattt aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt
     3841 agagtctttg attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata
     3901 ttcaggatat ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt
     3961 gataggaaat acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg
     4021 ttggaaagtg attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg
     4081 ggcaagaggt ggacaagtgg gttcatggtt tgcagatgga acttatggac atactggcgt
     4141 tatccgaggc ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat
     4201 gatttgtgga aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat
     4261 accaccaaaa tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga
     4321 accctataat tacgtgggga tgattcaagt tcgacaaggc aataagaata cagaggttat
     4381 ggaagcgact atagttgaaa atggtcttcc ctacgattta tcagaatgta aggtatattt
     4441 tgaatcagtt gtaggtggga aatatccagt ccaattagaa acaaaaattg tggatgctaa
     4501 aaaagggaaa atcaaatata tttttgataa atattccatg cagtgtttac atcgacaaac
     4561 agccaatttc attatattta aaggagaaga cttgattgga acaactcaag acttctctta
     4621 ttttgtcatt aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat
     4681 cgaagattta attgcggata tgacggactt tattaatgaa aataaaggcg attttacgga
     4741 ctggatgaat gaaaggaaag aagaatttga acgttggaga caagaacagg aacacagttt
     4801 ccaagattgg agagaaggac aagaatcaga ctatttggca tggtttgaat caatcaagga
     4861 tattttaaag acgattgatc caggtggagt catgttagcc gaactaacgg atgcgcgagt
     4921 agatattcaa ggagttcgtc atgcgtctat ttcagagcgt ttgctggcag atatggacta
     4981 tttatatcag aaattgcgag caacgctttt cacgattgaa tacggggaga ttgaagtgac
     5041 tgatattttg caggatgatc tcttttcaga taatcatgaa gtcgaaaaag ttggaactgt
     5101 agcattcccg attgaagaag gagccttgat catcgcaacc gttgatgatc caaagcaaaa
     5161 tgtgttcact cttgagaaag ttggggtgat ctaatggcta aaactaaacg aatgatggaa
     5221 acggatgaaa aaacgggtgt acaacgccaa ttctttccaa tcacacatgc ttccgcagtt
     5281 cttgggttag aagaaataat agcaggagaa gcaacagttt tatctgttaa tggaaaaatt
     5341 ggagccgtcg tcattactaa agaagatttg ggattagaga atgttctcac agaattaccc
     5401 tatgcaagtg aagaagatga cggcattatc acggcagaaa tgtatcaaaa aattttaaac
     5461 agtggagaag gtgactacgt gttgccagtt gccactatcg accgtttggg tggcataaaa
     5521 gttggtgaat tattgacgat tgatgagaca gggaaagtct ctgcagtcag acaatctgat
     5581 gtcaattttt cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta tactgctgga
     5641 gaaaacatca ctattgatga agatggaaca attagttcta caggtggtag tgggactggt
     5701 ggtgtcaatc agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca agcagaaaat
     5761 tacacaaacg aaagaattcc aaattttact tttgaaaaaa ttggggaggt atagagaatg
     5821 acagatatcg taaaaataaa acaaagcaat gtacaggttt atccacaaac tcattggaat
     5881 gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg ccaagcagca
     5941 acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa tgtaggaaca
     6001 tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga tcctggaata
     6061 aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc gtccacagat
     6121 aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa tgctacaact
     6181 acgacagcag gtttaatgtc agcaactgac aaagctaaac tcgatggatt agccaatatt
     6241 acatttgaga aagtggggac ggtttaatgg ctgatattgt gcaattaaaa gaagacggag
     6301 ttgctaaata cttaaaaacg catgtagaag ctattgatgg taaagaagca ttggtgcaga
     6361 cagatggaga ccaagccatt gcaggacata agaatttttc aggttctgta acaatcaata
     6421 ataaacgtgt tttgacgact gatgataaca gatatgaagt ggtaaatctg gttgtcacta
     6481 acggtaatac agggacagca aagctttatc gtgaaggaaa aacagtttct atatattttg
     6541 tggctttaaa cggaaaaagt agtggcggga atgattcggt tattttaact gttccagaag
     6601 gctatcgg
//
LOCUS       DACCWV010000139         6550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCWV010000139 DACCWV010000000
VERSION     DACCWV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.141"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            141..962
                     /locus_tag="IVW84_002451"
     CDS             141..962
                     /locus_tag="IVW84_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7918622.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            982..1542
                     /locus_tag="IVW84_002452"
     CDS             982..1542
                     /locus_tag="IVW84_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP7918623.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2296..3063
                     /locus_tag="IVW84_002453"
     CDS             2296..3063
                     /locus_tag="IVW84_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7918624.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3089..3634
                     /locus_tag="IVW84_002454"
     CDS             3089..3634
                     /locus_tag="IVW84_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918625.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3635..4201
                     /locus_tag="IVW84_002455"
     CDS             3635..4201
                     /locus_tag="IVW84_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918626.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4216..4464
                     /locus_tag="IVW84_002456"
     CDS             4216..4464
                     /locus_tag="IVW84_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918627.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4483..4722
                     /locus_tag="IVW84_002457"
     CDS             4483..4722
                     /locus_tag="IVW84_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918628.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4739..5323
                     /locus_tag="IVW84_002458"
     CDS             4739..5323
                     /locus_tag="IVW84_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918629.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5430..5711
                     /locus_tag="IVW84_002459"
     CDS             5430..5711
                     /locus_tag="IVW84_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7918630.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5701..6042
                     /locus_tag="IVW84_002460"
     CDS             5701..6042
                     /locus_tag="IVW84_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7918631.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2484 a          823 c         1347 g         1896 t
ORIGIN      
        1 actttcaatt attgtgaaat atttcccttt aattaatcaa attttttata aaagtaatat
       61 ataaaataaa ttgcttgaag tattatattt agatatcata atttatggaa gaaaaattaa
      121 ttaaaaagga tgagattata atggggttac tggttccgag agctaatact tatactatac
      181 ctttaacgaa acgccaatta attgctaaaa gatatcaaag gattactaga gccatcaata
      241 gagagttttg gaattctgaa agtgatactg ctcacagttt atacgtaggt tcttatggga
      301 gaggaacggc aattagtact agtgatatag atataatagt tgaattaccg atggcagaat
      361 ttgacagatt caaaaattat ttatcaaatg gaccgtctaa gttattacaa gttataaaaa
      421 acgcatttca ggaaatacta ccaaatagtg atattagagc tgatggacaa gttgttaaaa
      481 taaatttcca tgatggaatc aaatttgaaa tagttccagc ttttaatgaa aaagattatt
      541 tgggtgagtc aaaagggttt atttatccag attcaaacat gggagggaac tggaaagcta
      601 caaatcccaa aaaagagcaa gaagccatga aattaaagaa tacaaaaagt aataatttat
      661 tatatgctac gtgtaaacat tttcgacatg tacgagatac agaatttacg agttatcatc
      721 tttcaggaat agtaattgac agtttcgtat atgaggctat gggaaattgg aaattcgttg
      781 aaaataacag tgggggtcaa aacattagtt ctgtatctta tgagacagct ttattagaat
      841 actataactc acataaagtt atgggcggtt taaatttata ctctcctgga agcaatcaat
      901 ttgttaattc tgatagtagt atcatatgtt tagaaaaagt attaaaaaaa atagctttat
      961 aaagttttat ggaggaacaa catgggagac gaaaaaaagc gcttggaagt tcaattacgt
     1021 gaagcatatg gacgagtaac gtatacttac accactcatt taaagcaagc cgataatatt
     1081 atttctgaca ataaaatttt aaggtgtctt caaattggtt tgtcagccct tactactgga
     1141 ggggtactta gtaccattat tactgataat agtgagttaa cctttgttgc tggattatgt
     1201 tcaacaattt ctcttggatt aaacttatac tttaagaatt ttgatcttgc tgaagaagct
     1261 aaaaatcatc aaattgttgc cgataaatta tggttgatca gagaagaata cgtaagctta
     1321 ctaacagatt taaatcgttt aagtgttgaa gatattgtga aaaaaagaga tcaattaatg
     1381 aacaaaactc atgaagcata taaaaatgcc cctaaaacaa atagtaaaag ctatgaatca
     1441 gctcagaaag cattaaaatc tgaagaagag caatttttta cagaagagga attggataag
     1501 atgttaccag agcatcttag aaaaaagagt tctaaatatt gaactctttt tttatgctta
     1561 aaattttctt gtaatttttc tattcaatca ataaataatc tatatttaag aagaaatatg
     1621 atgatagaaa catctcctaa agccaccgca agccgtccta gccattgccc agtccgacat
     1681 cgctagatta gtgcatctct ccaaaaaagt tttctaaagc caccgcaagc cgtcctagcc
     1741 atcgcctagt gcctagtccg attttgcttg atcggttcca tttctgttta tttagtgggt
     1801 caacaccgta atcttaaata gccatcgcct atttaagatt actaggtctg ctttgaaggt
     1861 aaaaagtctt tcattcttca agacttttag gctttcaagc tacgcctgaa agcattgaaa
     1921 ttcatcgctc tttatttggt tttgccattt ataattcctt acgcccatta tctttttcgc
     1981 ctgtcatacc ttgtcagaag tgtttagagc ttatctcatc tttggattaa tacaagtggc
     2041 gtaaattttt ttctaaacgc aagcgttacg aaaataattt acttaaacac ttgtattaat
     2101 ccggcagata agaacgcatt ctaatcactc ccgacaaggc aagacaggcg aaaaagataa
     2161 ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa atagcgatga agaaaaaacc
     2221 acctccctct aaaacaatcc aaacaaaaaa gaattttgaa aaaccaaaaa tcaaaaacag
     2281 aaagaagcgt tcaacatgag agatagaatt ttagaagtta atcaagaaat ggtaaacaag
     2341 ttaacaaata tcagtgggaa ctatgaaaag tacgatggta ctttacttga taattatatt
     2401 tttcacgata cacaaaatat cagaattggc agaagtaaac cgagagcgtt cattctcatc
     2461 aaagagaaat atttgaatga atggaatagc acctatgaaa tggttatgac tgataacttt
     2521 gaaaaggtat tggatttcag aaatgctttg ttgggttctg atatgggaaa tagcagtctt
     2581 gaatctattg aagttgaaaa caagaccgag aatgaaggtg aaggattgat taagaatgtc
     2641 ctgaaaatcg aacaaaaata tatcagtgaa gatagaagtt tttatagagt cacaagctcg
     2701 gttggtctag caaactttgc tactaactta attggaaaca atgctagtga atctttgcta
     2761 gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata gcgaagtttt caaaggtgga
     2821 attaatcaat cagtagcgat accaagagag atttttcaac tagctttgct aaacaattca
     2881 gcaaaaatcg cagtatcaca taatcatccg agcggatatg aatttccaag tgaaaatgac
     2941 aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa ttggtattga attgttagac
     3001 catatcatcg taacaagtaa aaacacaagc tattactcat ttcaagaaaa tggttcttta
     3061 taaaaattag agaaaagagg aaaacaagat gggcgaaaga acacaagcgt taattacagt
     3121 caaatatggt aacagcaaaa cgaaagtaag cgtgtcaatt cattatcagt ggggatatgg
     3181 tcgattcatg ttgatggacg ttttgaatca aggaatatat ttaatcaata aagcatatga
     3241 cttacgagat gaatcaagag aagaattagc aaaaggaatc tttgatagaa gtacaggagt
     3301 tatcaatttt gtcacctatg aatctggagt gagtgcttat gatgaaacag ggtttggtgg
     3361 gcgctatgaa ccttatcaag aaacgattga agatgtgatg aagttttcag ataacaatga
     3421 tggctatgct catttagaga tggtgttcga tgaaaatgga tttagtctag atgataaaac
     3481 cagattgcga ttgtttgatt ttgagggtca acagatagga ctaaacaagt tttgtgaaag
     3541 cggaaaagag tttgctacga aagattttat taagggatac agattattgc tagaaaatta
     3601 tggaataaac ctagaagaag agagcgtgaa ataagtgttt gaaaaaagaa aagaccagtt
     3661 ccctaattta gaagcgaaaa gtcaaaaagt gattgatggt gaaatgattg aattattcca
     3721 aaaaagttat ggaaccatag aaactaccgc aagttgcaaa tactactttc aaggaaagag
     3781 aaacacgttt aaaggctttg ccgattctga tgaagaattg gcaaagaaaa tcattgattg
     3841 gcttgtcagc agtgaacacg tagcaagaga agataccgtt agaacaacga ttgagggtga
     3901 agtcctaaca ggtactagat gtgtttgttg taatcatgaa tttgtagaag atgaaggttg
     3961 ctaccgaatc aatgaatatg gtgattttat ttgccagagt tgccacgaga tagaatttcc
     4021 aaccgaaaga agttggtttg aatacatgct acaagattat gagatggaaa cagaaaatgg
     4081 tgaagtagtt ttttgtacca aagaggaatt gagtaagaga agcgatgatg aaattgaaga
     4141 ttggtgtgtg tggttagtag ggacagatga ttgcgttgca tattacacaa cagcagaata
     4201 ggagtgtgaa tcagcatgga aggaatggat tttatagatc atgaagattt gattgatttt
     4261 ggttatacat ggaaaggaat ggtaggtatt agcagaagtc ttgctaatgc cttttacgaa
     4321 agaaattatg cagtctatgt cctttatgat gacaatacag aaagccttgt tgatgaagag
     4381 tataaattgg atttagagaa tgttttatat gggatagaaa aagaagattt agcaaaatat
     4441 attttttcat ggttgggtca ataaacgaaa gagggtagca acatgacaaa attcaaaagt
     4501 aaaggtatgc aatggtgttc ttattattat gttgggaatg atgaagagct tttgagaaaa
     4561 gccaaacaaa aaggttatcc agataagatg ttaaaacaga ttggctatta catgccagaa
     4621 gagagaatag aaaaagaatc ttgtaaggtt ctttttgata gtgaacagct attagttttg
     4681 ttaactcaaa ataaattgat ttgtcaattt gaaaataatt aatatggggt gaagaaccat
     4741 gaatgagaat aagatggtat taagtgaata cgcaagattg agaaccatta attggttgaa
     4801 tggtatgcaa agtagtggcg cttttgatgg atttgatgag gagaatatgg actttgaaag
     4861 tttatcgaat gaggatatta gatattattt tatcgaatgg tattttacag aaccagatga
     4921 agaggtggaa gatgaaccag aattaatttt agaacgtcaa agaattttat tcagtgaaca
     4981 agatagtaag aggttgtatg gtttatatga cttacaactg aaaggattaa aagcgcagta
     5041 tgttgctgat agttacgatg atatggtaga acagggggca gatagggttg tcacttattc
     5101 agaagatgat gaagaggaat gggaaaacta ttcagatgat gagatatgct ttaatgatgg
     5161 gtatatgcct attgaaatca ccgaagaaga atatgataaa gtgtcggata gcgatgagat
     5221 tggtatgtgt acggttgtaa gtggattgga tatagatgtc caaagagtag aggttgcata
     5281 ccagaagatg aaagaacgta taaaaagtaa agtagaatct tgatttgatt ggattgtttg
     5341 caaagtagct aattaagagt ataatataat cataaatgta gttaatggat ataataatga
     5401 gtatattata ctcaaaagag gtgaacgaaa tgaaggtaaa aaatccaaaa gaaaaaagca
     5461 gaattcaagt cggaatagac aaggatttaa aagaaaacgc agaaatgata ttggaagagc
     5521 tagggttaaa tccaacaaca gcaatcacaa tcctttataa gcaagtggtc gctagaggtg
     5581 agtttccagt agagataaag ctaagcgaag aggaaaagca aggaatcaga ttgcaacagc
     5641 taactatgga tatgccagtt gatgttctag atacagatga aaagttagaa gagtggttca
     5701 atgaagccta atgagatagt aacggtctat gttgcattta cagataaaaa tggtggtaaa
     5761 agaagaccta ttttggtggt tagtgataaa gaagatagag tagaattttt tggaatcaca
     5821 agtcaatatg agaataaatc agaccaaata aagagagtgt attttcctat tgcagaatgg
     5881 gaaaaagtag gtctaaagaa gcaatcatgg attgacgtag ggtctttaaa ggctattccc
     5941 aaaagccgag agaacatatc atttaaaaag gttggtatgc tatcagctat tgatacgaaa
     6001 aagctaaata ggtttatcga gagattacaa agaaaagtct aacaaggctt ttcttttttt
     6061 gtttatatgt atattaagat atacgtttat ataaacgtat ttatgtatat taaaatacac
     6121 gtaaatatat acgtgattat aaaaattaag aaaatattaa gtattgttaa atagtgataa
     6181 ttagtaatat acgaatcata agatacattt tatatttaat gtatgtaaat aaataaaatg
     6241 actgacggat attgaatagt aataaataac aataattcag acggatacag tgagtgaagt
     6301 agcttgctac gaacgaacag gcagaatgaa acggtagttt cattagagca agtcgcttgc
     6361 gacgtagcgt taccactgat gtaactggca agttacatag agagagtttg cttgcaaacg
     6421 aacgttaccc acttgaatag aaaacaaaaa taacatatta gtgaattttg aataggagcc
     6481 acaactcaac caaccgcttg cggtgggtga gtcccacgga ggaaaagacg aacgaacggt
     6541 cgcgtagcga
//
LOCUS       DACCWV010000140         6503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCWV010000140 DACCWV010000000
VERSION     DACCWV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.142"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            23..760
                     /locus_tag="IVW84_002461"
     CDS             23..760
                     /locus_tag="IVW84_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7918632.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            774..1589
                     /locus_tag="IVW84_002462"
     CDS             774..1589
                     /locus_tag="IVW84_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7918633.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1602..2249
                     /locus_tag="IVW84_002463"
     CDS             1602..2249
                     /locus_tag="IVW84_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7918634.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2265..2921
                     /locus_tag="IVW84_002464"
     CDS             2265..2921
                     /locus_tag="IVW84_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7918635.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3083..3904
                     /gene="racE"
                     /locus_tag="IVW84_002465"
     CDS             3083..3904
                     /gene="racE"
                     /locus_tag="IVW84_002465"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7918636.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3907..5262
                     /gene="rph"
                     /locus_tag="IVW84_002466"
     CDS             3907..5262
                     /gene="rph"
                     /locus_tag="IVW84_002466"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7918637.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5263..5781
                     /locus_tag="IVW84_002467"
     CDS             5263..5781
                     /locus_tag="IVW84_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7918638.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5888..6388
                     /locus_tag="IVW84_002468"
     CDS             5888..6388
                     /locus_tag="IVW84_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7918639.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2138 a         1093 c         1440 g         1832 t
ORIGIN      
        1 ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg ataaatatta
       61 tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag aagttgtcgt
      121 tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca atggattaga
      181 gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata aaaagacaaa
      241 actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt tgtacccaaa
      301 taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc aagaccaggc
      361 aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag ataagaaaga
      421 ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg ccagaggttt
      481 agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg atccggaaat
      541 gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt caatgattgt
      601 cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct tcatggctga
      661 tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc caaatgaagt
      721 ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa aagatgcgca
      781 ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca ctagccggat
      841 gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct accaatgaaa
      901 tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt gagagcagaa
      961 ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc cttggtgaca
     1021 atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta ttgaaaaacg
     1081 gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa aaacaggttg
     1141 acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa ggaagccaaa
     1201 tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa ggttctacat
     1261 cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta gaaaactatg
     1321 cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg gataatgcga
     1381 ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga acgtttacta
     1441 atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa gctgttaacc
     1501 aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa aaatggtttc
     1561 caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa ttgatacaaa
     1621 cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt gtcttagcac
     1681 ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca aagaataaag
     1741 tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca ttattaatca
     1801 tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat ggattccaag
     1861 caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca gtacgttcag
     1921 ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt tttacctatt
     1981 cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc ccgccattag
     2041 gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta gctggtttag
     2101 atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc gatacgtata
     2161 tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta atggtttatc
     2221 ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat tttagcggcg
     2281 ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact gttgaagttt
     2341 cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg attcgttttg
     2401 cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata cggaatcttc
     2461 cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc attcagatga
     2521 acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg ttgtcggaaa
     2581 ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga ttatcaacgg
     2641 gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt aaatcaatga
     2701 tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta gcagtaatca
     2761 tttctttacc tgaactttct catcaggcac gtattattta tggacagaac accaattatg
     2821 tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac gcgctttctc
     2881 tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt cttttagcgg
     2941 ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa agagatttcg
     3001 attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac aatagggagg
     3061 atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta tcggatttat
     3121 tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat taccgaatga
     3181 aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac ccgcggaaca
     3241 ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa tcaagatgct
     3301 ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag ctgctctttc
     3361 tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa aaacacaaaa
     3421 taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt atgaaaaagc
     3481 actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca aatttgtttc
     3541 agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg cagaaacatt
     3601 agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc attatccatt
     3661 attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg attctggagc
     3721 agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca attcaccgca
     3781 aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt tcgaggaaat
     3841 agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat tgggaggaaa
     3901 ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga gcttcaaaca
     3961 aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac aacagttatt
     4021 tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg aacaggatgg
     4081 gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg tagagaaagc
     4141 agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg tcgttccttg
     4201 cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga ctgcgatgtg
     4261 atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt ggcattgaaa
     4321 ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat aaaagaacat
     4381 cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga tttggattac
     4441 caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc tggaaaattc
     4501 gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt gaatgaaatg
     4561 ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca tgctttgcta
     4621 acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat cattgcgaca
     4681 agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg atatcatgta
     4741 aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag cacatttgaa
     4801 gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc agtcttagca
     4861 gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta ttctgcacgt
     4921 tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata tgaattgaca
     4981 gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt cgcggcaccc
     5041 aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg tataccatct
     5101 ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa acaaacagca
     5161 gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc aatgaaaaaa
     5221 ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta tttagtagta
     5281 agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta tcgttctgaa
     5341 gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc attatgggat
     5401 acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca gaaaaaagtg
     5461 atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa tgtccgattt
     5521 ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt gttttttggt
     5581 catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa tcctggtagc
     5641 atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga aagtttagat
     5701 gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga gttggccttt
     5761 tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat aagctttatt
     5821 ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata gaaatggagg
     5881 ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg aaacgttttt
     5941 gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc atggtttact
     6001 tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg atcactttgt
     6061 aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa caatcagcat
     6121 ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc cgacaattga
     6181 tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt ttttaccggt
     6241 tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc ttaaagccgt
     6301 aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa tcgaagaatt
     6361 aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa tcaaaaaata
     6421 agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg ggacaaaagc
     6481 gtttaactcc aagaaataag aag
//
LOCUS       DACCWV010000141         6332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCWV010000141 DACCWV010000000
VERSION     DACCWV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.143"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVW84_002469"
     CDS             complement(99..1229)
                     /locus_tag="IVW84_002469"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7918640.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVW84_002470"
     CDS             complement(1878..3080)
                     /locus_tag="IVW84_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7918641.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVW84_002471"
     CDS             complement(3140..3343)
                     /locus_tag="IVW84_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7918642.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVW84_002472"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVW84_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVW84_002473"
     CDS             4880..5692
                     /locus_tag="IVW84_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7918643.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1887 a         1300 c          998 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aa
//
LOCUS       DACCWV010000142         6289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCWV010000142 DACCWV010000000
VERSION     DACCWV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.144"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(38..226)
                     /locus_tag="IVW84_002474"
     CDS             complement(38..226)
                     /locus_tag="IVW84_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7918644.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            496..858
                     /locus_tag="IVW84_002475"
     CDS             496..858
                     /locus_tag="IVW84_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7918645.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            910..2592
                     /locus_tag="IVW84_002476"
     CDS             910..2592
                     /locus_tag="IVW84_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7918646.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2709..4745
                     /gene="recG"
                     /locus_tag="IVW84_002477"
     CDS             2709..4745
                     /gene="recG"
                     /locus_tag="IVW84_002477"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7918647.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4794..5795
                     /gene="plsX"
                     /locus_tag="IVW84_002478"
     CDS             4794..5795
                     /gene="plsX"
                     /locus_tag="IVW84_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7918648.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5917..6159
                     /gene="acpP"
                     /locus_tag="IVW84_002479"
     CDS             5917..6159
                     /gene="acpP"
                     /locus_tag="IVW84_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7918649.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2089 a         1094 c         1434 g         1672 t
ORIGIN      
        1 aaccgttcac tatctgaacg gtccaagtat cattcaatta aacgcgttca acttttcctg
       61 atttcaaagc acgagttgac acccaaactt ttttaggttt tccgtcgacc ataacgcgaa
      121 ctttttgtaa gttaggttta acagtacgtt ttgttgagtt catagcgtgt gaacggttgt
      181 ttccactttt tgtcttgcga cctgtaaagt aacaaacttt tgccatggta atttcctcct
      241 ttgccttgat ttggagcaat agaataattc agctcatacc agactaacat atcatataga
      301 atggaaattt gcaagtacat tttcatagat acaattgaca attgagaaga ataattgtaa
      361 attaaacaag gttatacctg aaattttgca aggtttttga gtactttggg taactgcctt
      421 ttatttcaag ttgcgctatg ataaaatatg atagaagaaa aatgggagtt ttttacccgt
      481 taaggaggct cattcatggc tgtaaaaatt caaacgtcag ctggaaccat tgaaattacc
      541 aatgatgtaa ttgcaacagt tgttggtggc gccgcgacag atgtgttcgg tgttgtagga
      601 atggccagca aaaatcaaat taaagataat attactgaga ttttgcgtaa agaaaattac
      661 tcaagaggag tagttgtccg ccaagaagat aatggtattg ctgtagatgt ttatacgatt
      721 atcagctatg gtacgaaaat ctctgaagtt tcccgcaacg tccaagaaaa agtgaaatac
      781 aatctagagg cattattagg cgtaactgcc aattcagtta atgtctttgt acaaggtgtt
      841 cgtgtactgc cagactaggc aggagactgg ctggtaactc agcagtcgat ctgaaggagg
      901 attatctagg tgagtgtaac agaaataagt gcaagccagt tccaggaaat ggtccaggca
      961 ggtgcgaatc gtctgcaagt aaatgcagaa tacgtcaatt cattaaatgt attccctgtg
     1021 ccagatggcg atacgggaac aaatatgaat ctatctatga ctagtggagc taaggcagta
     1081 gtagattcaa cctcagaaaa agtaggagaa ttagcagcaa tgctttcaaa aggattgctg
     1141 atgggtgcgc gcggaaattc cggcgttatt ttgtctcaat tgtttcgtgg attttctaag
     1201 caaattccag atgttgtctc attaaatgct tccgatcttg ctgcagcctt cactcatgga
     1261 gttgaaactg cgtataaagc agtaatgaaa ccagtagaag gaacgatttt gacagtagct
     1321 agagaagcag cgaaagctgg agaaaagaaa gcaaaaagta ctgatgatgt cgttgaagta
     1381 atgacagctg ttgtaaaagg ggcaaagcgt gctttagcta aaactccaga cttgcttcca
     1441 gtcctaaaag aagtcggtgt cgtagacagt ggagggcaag gactgctgtt tgtttatgag
     1501 ggctttttaa gtgcattgaa tggagaattc caagcggatg aaacatatga accttctcca
     1561 gcagaaatgg atgatatggt caatgcagaa catcatcgca gtgtccaagg tcaaatggcg
     1621 acagaagata tccattttgg ttactgtaca gaaatcatgg taaaaatcgg agaaggacct
     1681 acagtcgata gtacattcga ttacgaagaa ttccgtaact atttagatgg tataggagat
     1741 tctttacttg tggtcaatga tgacgagatc atcaaagtcc acgtgcatac agaaaaccca
     1801 ggagaagtct tgaattatgg acaaaaattc ggttcattag tcaaaatcaa agtcgacaac
     1861 atgcgcttgc agcatgagac gattttggaa cacgatgaag aagtgaacga gtttgcgaaa
     1921 aaagaaacac cacgtatccc gtatggtatc gtagcgatcg ctgctggtaa aggcgtccaa
     1981 gagctgtttg aaagtttagg cgctaattat gttatcagtg gtgggcaaac aatgaatcca
     2041 agtacggaag acattgttaa agcgattcaa gaagtaaacg cagataaagt gattgtcttg
     2101 ccaaacaata agaacatctt catggcagct gatcaagcag cagaagtagc agatatccca
     2161 gtagcagttg ttccttcaag aacgatttcc caaggattga cagctatgct ttcattcaat
     2221 gatcaagctt ctttagaaga aaacaaagaa acgatgacag aaatgttgtc tagcgttgtg
     2281 agcggtcaag tcactcacgc gatcagagat acctcaattg acggtgtaga gatcaaagaa
     2341 ggagactttt tgggcatgat cgatggaaaa atcctgatct ctaacccaga tatcacagca
     2401 acagcgttaa gtacactaga acaaatgatc aatgaggaca cagagatcgt aacgatcttg
     2461 gcaggtgatg aaggaacagt tgaacaagca aatgaatttg ctgagaaact aacagaaaaa
     2521 catcctgatt tggaaatcga gatccaccaa ggagatcagc ctgtctatcc ttatctattt
     2581 tctgctgaat aatttctgtg tcagaatatc tttctaacac agtattcatt cataaaaggc
     2641 cttcggttag tgatcaggta tgatcgctga ccaagtcctt tttttgttaa gggagggagg
     2701 aaaaatttat gcgttcttta gatgatccaa tcactatgct ggcaggagtc ggagaaaaaa
     2761 gagcagctag tcttgccagc ttgggcatcc aaacgattga agacttactg acttattatc
     2821 ctttccgcta cgaagatatt caggaaagaa atctaaacga gatccaagat caagaaaagg
     2881 ttactttgaa aggaatcgta gtctcgcctc ctgtgatgaa tcgatttggt tataaaaaaa
     2941 gccgattgca atttcgtatg atgcaagaac atgatgtgtt caacgtttct ttttttaatc
     3001 aaccttatct aaaagaaaaa gtcattttat cggaggaaat cgccgtatac ggtaaatggg
     3061 atgcaaagcg taaagcattg aacgggatga agattttagg ttctcaaaca atggatgact
     3121 tttcgccgat atatcacgtc aacaaatcaa ttcgtcaaac gacattagtt gatctaatca
     3181 aaaaagggtt tcaagaatac ggcacattga ttgaagaaaa tctgccaaac aaccttgtgg
     3241 agaaatatcg actgcttgac cgagcttctg ctgtaagaag tatgcatttt ccaaaagacc
     3301 acgaagaaaa ccatcaagcc aaacgacgtg tcgtttttga agagttcttt ttatttcaaa
     3361 tgcgtcttca aggattgaaa aaagcagaaa aagcagaaac gaatggtttg gagatccttt
     3421 atgatgtcca aaaattgaaa gaatttactc aaaagctacc ttttgaactg acaaatgcac
     3481 aaaaacgtgt aacgaatgaa atctgtcggg atcttcgcag tccgcagcat atgcagcgac
     3541 ttttgcaggg agatgtaggt agcggaaaaa cagtggttgc cgcgattgcg ttatacgcta
     3601 ccgttacagc aggtttccaa ggtgcgttga tggtgcctac agaaatcttg gcacaacaac
     3661 atatggaaag cttgcaacag ctgtttgatc ccaatgaagt aaccatcgca ttgcttacgg
     3721 ggtcaacgaa agcaaaagaa cgtcgtgagc tccttgaaaa actagagcaa ggggaaatta
     3781 acatcgtgat cggcacacat gcattgatcc aagatggagt cgaatttcag aatttaggtc
     3841 tagttattac ggatgagcag caccgttttg gcgtgaacca gcggaaagta cttcgtgaaa
     3901 aaggctggcg accagatgtc ttatttatga cagcgactcc gattcctaga acattagcta
     3961 ttacagcata tggtgagatg gatgtatcta tcattgatga acttccagct ggacgtatcc
     4021 cgatcgaaac acgctgggta agaacacctc aattggacag tgtactggat tggacgaaaa
     4081 aagaactggc aagaggacat caaatgtatg tcatctgtcc gctgattgaa gaatcagaag
     4141 cattagatgt aaaaaatgca gtggaaatct atgaaaaact tcgtgaacta tttgcaccaa
     4201 aatatgaagt tggcttgtta catggtaaaa tgaagaatca ggaaaaagaa gtaatcatgg
     4261 aagccttcaa agacaaccag atgcagatat tagtttcgac tactgtaatt gaagtagggg
     4321 taaacgtgcc gaacgcgacc gtcatgctga tcatggatgc ggatcgattt ggattggcac
     4381 agctgcatca acttcgtgga cgtgttggac gcggaagtga tgcttcctac tgtatcttag
     4441 ttgctaatcc taaaaatgaa ctaggtgtgg aacggatgaa aatcatgaca gagaccacca
     4501 atggttttgt actaagcgaa aaagatctgg aattacgtgg tcctggtgaa gtatttggct
     4561 tccgtcaatc tggtctgcct caatttgcga ttgctgactt ggtgacggat ggaaatgttt
     4621 tggaggtagc cagagaagag gctgcagccc tttggcagat aaaggattgg cagatgcttc
     4681 ctgagtatca gcctttagcg caaagcctcc aaatagaaaa tcaggataat cgttatttcg
     4741 attagcagac tgtgctatac tttatactga tgacggaaaa aggagtggaa acaatgaaaa
     4801 ttgccgtaga tgcaatgggc ggagatcacg cacctcaagc aattgtagaa ggcgtggctt
     4861 tagcgcagca agattttcca gaagtagaat tcttacttta cggaaaagaa gcagaaatca
     4921 agaaatactt aacaaatgaa aaaaacatca cgattatcca tacagacgaa aaaatcaata
     4981 gtgatgatga gccagtaaaa gcaattcgac gaaaaaagac tgcatcaatg gtccttgctg
     5041 cacaagcagt gaaaaacggt gaagcagatg ctgtattttc agcagggaat acaggcgcgc
     5101 tactagcagc agggttgttc atcgtaggac gtatcaaaaa tatcgaacgc cctggcttga
     5161 tgtctacctt gccagtagta gggaaagaag gtgccggttt cgatatgctg gatcttggcg
     5221 ctaacgcaga aaacaaacca gaacatttgt tgcaatacgg tattttaggt tctttttatg
     5281 cacgtaaagt ccgaggtatc ggacgtcctc gtgtcgcact cttaaacaac ggaacagaag
     5341 caacaaaagg tagtgaagtg acgaaaaaag cctatgaact ccttcagaat gaaacaagct
     5401 tgaattttat tggaaacgta gaagctcgtg agctactcaa tggtgtggct gatgtagtgg
     5461 tcacagatgg atttacaggc aacgctgttt tgaaatctat tgaaggaaca gcaatgaata
     5521 tgatgaatct attaaaatct gctattctaa atgaagggat caaaggaaaa atgggcgcta
     5581 tgcttttaaa agacggctta cgttctttga aagcagaaat ggattattcc aaacatggcg
     5641 gtgccgtttt gtttggcttg aaagcaccag tcattaaaac tcatggagca acaggccccg
     5701 atgctgttcg ctatacgatc cgccaaatcc acacaatgct tgagacagat gtagttggac
     5761 aattagtcga acaatttgaa aaaagcgaag aatagtattt ggatatctga agcaatccgt
     5821 tccattgaaa aaaaggtgga cattcctgaa aaatgccagt aaaattagcc atggtgtagg
     5881 taattgacaa gtgtatgatt cttggaggtg aaaaagttga ctcgcgaaga agtatttaac
     5941 aaagtagcga aaattatttc aaatcatttt gagatagata ccgataaagt gacggatgaa
     6001 ttgaacatca aagatgattt aaaagctgat tcaattagta ttatggaatt cgttttagag
     6061 cttgaagatg aattcggtac ggaaatttca gatgaagacg ctgaacaaat tgaaacagtc
     6121 ggtggcgcag tagattatat cagcagccat cttaaataat ttcaaataaa agcaaagaag
     6181 gataaaaagc tgcatacagc tttttatcct tcttttttta atctttttga cacgtaaaag
     6241 tcatttttct gataattctg gcgataaaca gaaattatgt tgcaaagta
//
LOCUS       DACCWV010000143         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCWV010000143 DACCWV010000000
VERSION     DACCWV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.145"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVW84_002480"
     CDS             725..811
                     /locus_tag="IVW84_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7918650.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVW84_002481"
     CDS             complement(932..1621)
                     /locus_tag="IVW84_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918651.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVW84_002482"
     CDS             complement(1628..1906)
                     /locus_tag="IVW84_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918652.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVW84_002483"
     CDS             complement(1866..2780)
                     /locus_tag="IVW84_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7918653.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVW84_002484"
     CDS             complement(2762..3142)
                     /locus_tag="IVW84_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7918654.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVW84_002485"
     CDS             complement(3592..3879)
                     /locus_tag="IVW84_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7918655.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVW84_002486"
     CDS             complement(3899..4123)
                     /locus_tag="IVW84_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7918656.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVW84_002487"
     CDS             complement(4763..5296)
                     /locus_tag="IVW84_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7918657.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVW84_002488"
     CDS             complement(5317..6159)
                     /locus_tag="IVW84_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7918658.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtcgatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCWV010000144         6049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCWV010000144 DACCWV010000000
VERSION     DACCWV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.146"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..497)
                     /locus_tag="IVW84_002489"
     CDS             complement(<1..497)
                     /locus_tag="IVW84_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7918659.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(610..1176)
                     /locus_tag="IVW84_002490"
                     /pseudo
     CDS             complement(610..1176)
                     /locus_tag="IVW84_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(1510..1597)
                     /locus_tag="IVW84_002491"
     tRNA            complement(1510..1597)
                     /locus_tag="IVW84_002491"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1563..1565),aa:Ser,seq:cga)
     gene            complement(1742..2308)
                     /locus_tag="IVW84_002492"
     CDS             complement(1742..2308)
                     /locus_tag="IVW84_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7918660.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            2563..3894
                     /locus_tag="IVW84_002493"
     CDS             2563..3894
                     /locus_tag="IVW84_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7918661.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3860..4210
                     /locus_tag="IVW84_002494"
     CDS             3860..4210
                     /locus_tag="IVW84_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918662.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(4302..4721)
                     /locus_tag="IVW84_002495"
     CDS             complement(4302..4721)
                     /locus_tag="IVW84_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7918663.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(4722..5066)
                     /locus_tag="IVW84_002496"
     CDS             complement(4722..5066)
                     /locus_tag="IVW84_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7918664.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            5416..6012
                     /locus_tag="IVW84_002497"
     CDS             5416..6012
                     /locus_tag="IVW84_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7918665.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1683 a         1107 c         1037 g         2222 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctcgttcca tttgatagcg agcgataatt gttcagatta aataattgat aatcaaatgg
      661 cggattattt gtcaacacac cgacaggatt atcatataaa tgaagtccat ctttcatact
      721 ttcaattact atcgattttt ctttatcagc taataaccag tgtaaaggtg acaagggtag
      781 tttttcacta aaatcgatat tgactagatt gatagtagcc aataattctt tagcttcgtc
      841 tatggttgag cattgcccta gtatccatgg aataaactca aatggtgcaa cgttatcttt
      901 tccttcttgt aattctttat aatcagcatt gccaggaaaa ttcagtcctg ccatactcaa
      961 ccctttttca tttgtagcct cataatagag tggatagttg gataccccag ctgctattcc
     1021 tatcattgca taatgtttat ctaaattttt tacctttcta aaatcgaaac gataatttct
     1081 tggagtaata gtgaccactt cattataaga catctcataa tcaaaattcc ttccaaaata
     1141 atggtctttc gttgcataag tgattgcggt acacatcgtt tcccctccta aaacaaatcg
     1201 tttttacact tttatcttac atctagtatt ttttttatca aaaaaaggcc aaagaagatt
     1261 gatttactag catttataaa aaatagtact cagggggaat caaaaaatat tgtaggttaa
     1321 aagtacaaca ataaggggct gtgacaaaag tcttgtcaca gccccttatt ccgaacaaac
     1381 ggtgttcaaa agcttgcatc tgcggtaata agcccaatca aacgaaaaaa taggaaaagc
     1441 tatttttgtt tggccgttct tattacttga tgcaggacag cttcttcaca acctctaatc
     1501 gttgatataa ggaaggtaca ggatttgaac ctgcgcgccg gtattagccg gttcgccgga
     1561 tttcgagtcc ggtgcattac cactctgcca accttccgta agtactattt ttaaactttt
     1621 ttcacgaaat gtcaagccta tttctaatag ataaataaca gaaatcgctt tttttagcaa
     1681 aaaagtccat tgacagtttg cagccaacgg acttaccgat caaattcata ctgatagaac
     1741 actatctgat tatttcagga ttatctaatg ctaaaatcga gtcaattgtc cgtttgatca
     1801 gtgcaatgat ttgttcttct gttggatgca tgatgtttga tgacatcaat gacgctaagc
     1861 cagtcactgc tacccatgta ccagtatgca gagcatcgac atattcatcc gataattgat
     1921 tgtatctttc atcttcttta actatttcac ggaaataatt gtatgaaaaa tcatgcattt
     1981 tctttccgtc cccatgttca tcaatgtaca atgccatgta aagactatga tgcttttttg
     2041 caaagtagat atagttcaaa ccgagatcaa tgattttatc accagtacgt tcaataggga
     2101 aaacatcttc ttttagttgt tcaaagatag aatccagcaa cgtatttttt aaatcttcca
     2161 tgtttttgaa ttccagataa atcggctgtg tcgaaatccc catcttttta gcaatatttc
     2221 tcgcagtaaa tcctttaaac ccttcctttg caacaacatc ataagcagct tttagaattt
     2281 gatccttggt atagactttt cttctcatta aaactccccc acttctttac tgcagtatat
     2341 gttgttttat tgtaccctat ttttagcatt ttttgcaaac attttactca aaacgttata
     2401 ttttaaattt ttttcagtct tctttaactt ttttttgcaa aattaaaaaa atttggttaa
     2461 attcaatggg ttcccttaca tttttagttt tgacctaaat gatagttgtc caaatctttc
     2521 atttcaagtt atagttaaag aaaatgaaaa agaaggtgtg taatggaaaa cgaaactgac
     2581 gttatttata ttcatcctca aaaacgaatc gtttcacaga aaagaaagta tttttatctt
     2641 ggttttacag gtgttttttt tctttttatt ggcttgcttt ctaatactcc tactgataat
     2701 tggagtggat tgctaactat tcttacctcg ccaagcaatc ttttgacaga ttattttgct
     2761 ttaggtgttt tcggaagcgc attcataaat gtagggattt tgaccttact ttctgtgctt
     2821 ttagcttatc ggcacaaggt gattttaaac ggtcccttat ttgcttcgat tctaacagtt
     2881 accggtttct ctttttttgg caaaaacctt tataattcta tctcaatcat tttaggtgtg
     2941 tatctatacg ctgtatttgt caataaacct ttttctcagt acatcatgat cggactgttt
     3001 ggtagcgtat tatcaccggt cgttagttat atcacttttg gtatgcgttt tcctttactg
     3061 ataggtattt tattggggaa tttagctggc atagcaattg gtatacttct tccgccctta
     3121 gctgctcaaa ctttagtttt ccatcgtgga ttcacacttt ataatattgg attcacctct
     3181 gggttgattg cgatgacttt tactgctgtt ttgaggttat tcagctattc aatcgtcgaa
     3241 aatacattgg ttttcaatga atatcacttt cctctcattt ggattatttt tggctttttt
     3301 tcgctgacag tcggaattgg tttctattat aattcctttc ggctttcggg gattcgagaa
     3361 attttcgatt catctggaaa attgacaact gatttcattg ctaattctgg tattggtgct
     3421 actttgatca acatgggctt agttggtctg atgctgtctt cttatgtttt gctggttggc
     3481 ggtcagttaa atggacctgt catcggtgct attttatctg cggtgggttt cagtgctttt
     3541 ggttgtcatt taaaaaacag ttttccgata ctagtcggaa tatttattgc ttctttgttt
     3601 ggaacttttc atgagatcac cagtactggc atgcttgttg cagctgtgtt tgggacaggt
     3661 cttgcgccca taagcggttt ttacggcagt ttttacggtg taatagctgg tgtgctacat
     3721 attgctcttg tccataacgt cagcactttg cacggaggac taaatctata taacagtggt
     3781 ttttcaactg gtttcgttgc aggtatcctt gtaccgatac tagataactt taccgctgtc
     3841 agaaaggaaa aaaagacact tggaaaaaga attatcaaaa agaatcatcg atgagcttct
     3901 tacttattta tattcccatg aaataactga aatacgtatt ggagtagaat ttgctgagga
     3961 agggcttttc atagagatac agggtacggc agaaactcag ccagaagatt tagatgtact
     4021 tgctgatttg ctgaacaatc ctcgggaccc tagtatggaa atctactatc aagaactatt
     4081 aggtaatccc cgacatgatc gggaagatta tcatctgtta ggtagtttga ttgatgacgt
     4141 ggaaatcatg tatgcccagc cgattatcaa tattaagatc tttcgacgtg cttctcattc
     4201 ctattattaa ccctattttc tatttataaa agactgaatc aaaaaagcag ctcctgaaag
     4261 ttcgattaaa atctactttc aggagctgtt caagcttgac attattgatt ttccaatcct
     4321 tcatacactt catctgttac aggttctaaa aactctgaat taccagcagt gatcgccaaa
     4381 tgttcaaacc aactgtcttt tgtcgcacca tgccaatgtt taatcccttt gtgcgttacg
     4441 acgacatctc cagccattaa tcgtcttgct ggctttcctt cttcctgata ccagccttct
     4501 cctccggtca caagtaagat ttgatagccg tctgtatgga catgccagtg attgcgacaa
     4561 cctggttcaa aagttacatt acctacacca acagaaactt cgggatcatt cactaacata
     4621 ttcagatagc tttgcccggt aaatacttga gcgtaagctt cgtttttttc acctgctgga
     4681 aaaatcacgc cattgtgtat ttcttcgtgt tttgtcatca tttacgcctc tttcttatct
     4741 tcgctatttt cttctaaata gatttctttt gctaaactaa aagctgacca agcttttggc
     4801 cattctacat aaaaagctaa atgggtaatg atttctgcta tttcttcact agtaatgcca
     4861 ttttgcttgc caatcttcag atgagctggc aattgttcaa aatttcctcc tgaaattaat
     4921 gcagatatgg tgatgatact acgctgatga gcagataatt cttctctgga ccatacttgt
     4981 ccaaataaaa catcatcatt taattctgca aattttggtg caaaatctcc taattgatct
     5041 cgtcctgcgg tttgtttttc tgccatttct cttattctcc cttcacgact gttaggtgat
     5101 tcaactatgg ttctaaggca actttatttt ctattgtttc ttcgcatttc attttaaatg
     5161 tagtctttca gcctgtccaa tgcttagttt attcagtcga tatgcttttt aagcatagtt
     5221 gctaacgctt tttttatttt aaaaaaaaca agttattaaa taagtcttta gttggatgtt
     5281 tttttgagat caatctttta tacttatgat gaacttacaa aattgtttgt tttctgtcat
     5341 atcaaaaaat ggcgcagtta gttgcttttc tagtaaagta tagttaacaa agaacctcta
     5401 aggagttgat gaataatgaa tattgctgcc tctcgaaaac tgataaattt catatccatt
     5461 atcggattag gcctatcaat cgcgctgacg atttatttta ttaatttagg tgttttcaaa
     5521 gatttgaatg cgttaagagg attagtaggt gattccatca ttttaggacc gattattttt
     5581 gtcttcatcc aaatcctgca agtagttatt ccaatcattc ctggagggat cagtactgca
     5641 gctggtgtcc tgatatttgg cccttatgct ggatttatct ataactatat tggaatctgc
     5701 attggttcga ttatcatttt tctgctaggg cgtcgttatg gaaagccatt catactttcc
     5761 atgattagcg ataagacata taacaaatat attggctggc tggataatca aaaccgtttt
     5821 gagaagctgt ttgctctagc tatttttctt ccagttgctc ctgacgatgc gttatgtttg
     5881 atggctggat tgacaaacat gtcagtgaaa cgatatacac taatcatttt gattgctaaa
     5941 ccactgtcga ttttcttata tagtatggcc ttgatttatg gaggtcaata tctaagtggt
     6001 cttcttggat aatatttatt ttgtaaaatt agtcattgat tgacaatgt
//
LOCUS       DACCWV010000145         6007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCWV010000145 DACCWV010000000
VERSION     DACCWV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.147"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="IVW84_002498"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="IVW84_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7918666.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="IVW84_002499"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="IVW84_002499"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7918667.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="IVW84_002500"
     CDS             1945..2643
                     /locus_tag="IVW84_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7918668.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="IVW84_002501"
     CDS             complement(2811..5450)
                     /locus_tag="IVW84_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7918669.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1689 a         1317 c         1104 g         1897 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gcttttt
//
LOCUS       DACCWV010000146         5926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCWV010000146 DACCWV010000000
VERSION     DACCWV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.148"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(342..1241)
                     /locus_tag="IVW84_002502"
     CDS             complement(342..1241)
                     /locus_tag="IVW84_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7918670.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1262..1534)
                     /locus_tag="IVW84_002503"
     CDS             complement(1262..1534)
                     /locus_tag="IVW84_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP7918671.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1557..2963)
                     /gene="lacG"
                     /locus_tag="IVW84_002504"
     CDS             complement(1557..2963)
                     /gene="lacG"
                     /locus_tag="IVW84_002504"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7918672.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3105..4811)
                     /locus_tag="IVW84_002505"
     CDS             complement(3105..4811)
                     /locus_tag="IVW84_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918673.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4811..5128)
                     /locus_tag="IVW84_002506"
     CDS             complement(4811..5128)
                     /locus_tag="IVW84_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7918674.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5156..>5926)
                     /gene="lacD"
                     /locus_tag="IVW84_002507"
     CDS             complement(5156..>5926)
                     /gene="lacD"
                     /locus_tag="IVW84_002507"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7918675.1"
                     /translation="FILYLKLCNRTDKEAGLLLAYEKTGYDTSSTKRLPDCLDVWSAK
                     RIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPFFLEILAYDEEIS
                     DAGSVEYAKVKPRKVIEAMKVFSDLRFNIDVLKVEVPVNVKYVEGFADGEVVYSKAEA
                     ADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLCGRATWAGSVEPY
                     IKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
BASE COUNT         1872 a         1207 c         1050 g         1797 t
ORIGIN      
        1 acgctatatt taagatttca aacaactata ttaataggaa gaaaatttca tttttcctct
       61 tagttctata aaaagtgtgg ctaatttgag gcaaaatatt aaattaaatt tgccacaaat
      121 taatggctcc actattattt taaatcaaga agtagtaaat aatcttattt tttactacca
      181 gactgtcttt agatattagc ttttcgtcac ccactacagt tgacatagac ccacaaaaaa
      241 cttaaaaacc ttgcgatgta agactggcac ggagaagacc aatctatcat cacaaggtaa
      301 gagtattcaa atcttattga agactcaaat aataacaaat tttaattaag gatagtaata
      361 tcatagattt ttctagaagt ttcttgaggt gaaattttcg ttacctgtct tttatcttct
      421 aagaaattac cttcttcaag tgaagtggag agaccactcc aaggttccaa agcaatgaat
      481 ggacttttat tagttgtaga ccacagaatc aagttcggaa aatcatcaaa atctactcga
      541 agacctttac cagacttcct tgaccgaagc gttacgctac gagacttgag ctggtcaagt
      601 gtaatggcat catgggaaaa aagtgagtag tccaaatcga gaattttttg attttccaaa
      661 aaaggtgtcc gatcttgcaa atctaataat cctgtctcag gaaaactctt tggtatcgag
      721 caagactcaa cctcactaaa ttcaatataa taatcctcat agttttcacc ttctactaaa
      781 ggactattga atgctggatg ggctccaata aagtaaggca ttgttttttc catttccaag
      841 ttcgttacct gaaattcggt tctaattgat ttaccattca aggtataaac aactctcagt
      901 tcaaactgat ataggtaatt atcaagcatc tcagcatttg gtttaatact aaaggtcacg
      961 ctattatcat tgacttcttc cagaataaat tcttcttttc gaacaaaacc atgtctacga
     1021 ataagacctg taaaaaaagg tctttcttga ggtctataaa tagcccaatc atttcgtaaa
     1081 ctaccacaga taggaaatag aataggcgcc tgtccattcc aataatttgg atcagcttgc
     1141 cagagatact ctatcccatc cttgtcttta atcgaagtca gttgaccacc aagtgtttta
     1201 aactgaacag taagatactc gttttttaat tcgatagtca caactaattc ctttcttttt
     1261 attattggaa aggcaaataa tttacctcga agtgaatagt ctcacgtatt tctacatcga
     1321 gtgattgtaa aaattcccat tcaaatgctg ataactctaa atcagaatgg caaatccact
     1381 cttttccagt tgaaacatac catttaccac attttagcaa cggggtagct tcaagctcag
     1441 gaatcatagg acaatcttcg aaactaacaa tatatacctt ttcaaagcct ggtctaaata
     1501 agtgttgtac tttttctaaa gtatcggata acaatttttt acattttcaa gtaatattac
     1561 tctatcactt gagtttctgc tactttctta taccaatgag ctgatttctt aggatagcgt
     1621 tcttgcgtgt caaagtctac atagaatagt ccataacgtt tttcgtatcc gtttgaccat
     1681 gagaagacat ccataagtga ccagatgaag taacctttaa catttgcacc atccgcaatt
     1741 gcgtctgata aaacttccaa gtgtttcttc acgtaatcaa tacgaccatc atcataaacg
     1801 gtattatcta caaactcatc tttgtatcca agaccgtttt cagtgatgta aatcttcttg
     1861 tagtttggat aatcattttt cactcgcatg atttggtcat acaagccttc aggataaata
     1921 atccagtccc aatccgttct aggcacataa tctggtgcga ttcggcgacc aacaccttta
     1981 atctggtact tagaacttcc tttttcaccc ttaccattgt gaatgatttc agtctcacca
     2041 tcaaaggctt gcatccaatc actcatgtag tagttgatac caaggaaatc gttcaaatct
     2101 ttagctgctt caagtgcttg gaagtcttca tcacgaagat caagttctcc accattttca
     2161 gctaggatgt ggttgacccc ttccatcgtt ttatctgaat agtgtccaag gtaggttgca
     2221 tccaagataa atttgttatg gatgatatct tctaactcag cggcacgaac atctgctgga
     2281 ttttctggat cataagggta tttggttggc aacgcatgta ctacaccaat ttcgccttta
     2341 taacctttat ctttatacaa tttcacagca cgtgcatgag aaaccatcat attgtggtgt
     2401 gattggaaga ctttggcaag atcatattga atgcctggag ggaatttacc aaccaagtat
     2461 tgaccatctc caattgggcc aatttcattg aaagttgtcc agtagtttac ttctgggaac
     2521 tcttcaaaac agaaagcggc ataatctaca aagtgttcta tattttcgcg gtttaagaaa
     2581 tctccatctg agtgaagaac ttctggtgtg tcaaagtggt gcaaagtcac gaatggctca
     2641 acatgacgtt tgtggcactc ggcaaaaaga ttatggtaga attcaacacc tttttcattt
     2701 acttcgccat agccagttgg gaaaatacga gaccaggcaa ttgaaatacg aataccatta
     2761 acgccatatt tttcagctaa ttctaaatca acaggatatt tatggtaaaa atcacttgca
     2821 ggctctgctg tgtaccaata attgtcttca agatacttat cccatgcaac tgggccttta
     2881 ccatccgtgt gagtcgcacc ttctgcttga taagcagctg tagcaccacc aaaaataaaa
     2941 tctttaggaa gtgttttagt cataactata aatcctttca aaaaaagcat ttgagacgat
     3001 attctgagtt ccggaaacca aggaaatgaa atgcaatgat atggttaaca atattataag
     3061 gaactgaaag aataatctcg tctcaaataa cttttacaaa tttattaatc aaactgttgt
     3121 tgaacaaatg caagagcacc ttgtccatca cgtgtcaatt tgatatattg agctccttca
     3181 gtttttgcaa gtttaatgcc caatttatcc gtttcagcct tcatatcatc gaagtttgaa
     3241 gcgacctgtg gagccaagat aaccaagtca aactctggca acatttcacg gtgagcaccg
     3301 taactacccg ctgccgcttt gactggaaca ttgtattctg ctgctgcttt gttcaaggca
     3361 ttggcaagca agccacttgt acctccacct gcacaaagaa caaggacatt agtttctttt
     3421 gtgatattat tgtttgctgc tacatcttct ttagcaacgc ctgcttttcc aagaacggca
     3481 tctgctttag cagtattgaa gtttgcagct actttttctt taagagcatc gtttgtttta
     3541 ccagaacgtt cttcttcaag aatttgttca tcgtatacct taacaaatgg gtaataaatg
     3601 atagtatcaa caactaccaa gagtccggct aagataaatg atagaacttg gaagttagta
     3661 ccgagtacaa ttcccaacgg accaggagta acccatggaa ggttggcaga gaatgagttc
     3721 atgttcaatg tgtcaacaaa gaatttaaag atccaaacgt tgacaatcgg agcgaaaatg
     3781 aacggtacaa agaaaatcgg attcaatacg attggtgcac caaataagat tggctcatta
     3841 accccaaaga atgttggaac aactgaggca cgtccgatgg cacggttacg ttctgattta
     3901 caaatccaca tgaacaagaa tggaacaatc aatgtagctc ctgttccacc catggtaaca
     3961 ataaacattt gagtaccaga agtgataact ttatctgcat gttgtccagc ttggataaga
     4021 tgcaagttgg catcgatatt ggcataagtg atagcagcga ttgctggttc gacaattgaa
     4081 ggaccgtgga taccaacaaa ccagaagaag gcataagcac caaagataag tgtaattcca
     4141 aggtaaccat ctgcagctga gaagagagga gcaagaacgg taccgattga ttctgcaacg
     4201 gttacaccaa gagttccctt aacaatgagt tcaaatccat aaaggagaac aaccgctaca
     4261 gtgaatggaa tcaagtcttt aaatacttga gagatatttg gtggaacctc ttcaggcata
     4321 cgaatagtaa cattattttt cacacagacc ttgtaaacat taactgtcac aaaggctgca
     4381 ataaatgctg tcaaaagacc ttttgttccc gtgaaggctg tcaagaagcc tccttctttt
     4441 gcaggctcag ctgccatcaa aaggaaccct accatagacg ctagcatcgt agataagaag
     4501 ttaatctgat tggtcgcagg taggtcacgg ttcattgagt ccgtcaaagc tttagcggta
     4561 gtaccaccaa caaagaatgc taggataccc atcgaatagc tataaggagt catcaagaag
     4621 gtctcaatgt cttttgacca gtggaatccc caagcatttg ggacataggc aataaggata
     4681 aagatagatg agaaaaggat gactggcata ccagcgataa atccatcacg aatagcacga
     4741 agatagatgt ttcgagagat tttctcaaag aacggtttcc ctttctcaat aagttcaatg
     4801 agtttatgca ttattttgct cctcttttat aaagttcgat gaggtgatga atcacatctt
     4861 ttaataagac agtagtcatt aagtggtcct gaccatgcat catggtaacg ctgtatggaa
     4921 gttcctcccc agaagcttct cgagccaaca tagctgtctg agaattgtga gcctctgcaa
     4981 tacagcttcc tgcttctact acaagactat ctgccttagc gaaatcacca ttttcagccg
     5041 ctttaagcgc ttctaaaagt ttagagcgag catctccagc ataagcaaca atttcaaacc
     5101 ctaagagagt catctcttct ctgttcataa caatatcctc cgttttatgt ttttactata
     5161 ctttatcagt ccatggacta gctgttttaa caagaacttt gttaagttca tcaatatttt
     5221 cgaatccagt agtacgcaac cattcacgtg ctgctttttc accttctttg atgtaaggtt
     5281 ctacagatcc tgcccaagta gcacgtccac aaagcacacc attaaacttg gcacctgagt
     5341 cgtgagcaaa ttgcaatgtt tcttggaaca atttagcaga tacacctgca cttaggtaga
     5401 tgtatggaag gttagtcgct tcttcttgtg ctttaaagaa gtcagcagct tcagctttac
     5461 tgtaaaccac ttcaccatca gcaaaacctt cgacatattt aacgttaact ggtacttcta
     5521 ctttaagaac atcaatgtta aagcgtagat cagagaaaac tttcatcgct tcaatgactt
     5581 tgcgaggttt cacttttgca tattcaacag aacctgcatc tgagatttct tcatcataag
     5641 caaggatttc caagaagaaa ggaatatctt cagcgacaca ttcagaacca acacgttcaa
     5701 tataagcttg tttttgttgg ttaagttcat ctgagctgtc tacatcatag taaagcaaga
     5761 acttaacggc atcagcgcct tgctctttga tacgttttgc agaccaaaca tccaagcagt
     5821 caggcaaacg ttttgtactt gatgtatcat aacctgtttt ttcataggca agaagaagac
     5881 ctgcttcttt atcggttctg ttgcaaagtt ttaaataaag aataaa
//
LOCUS       DACCWV010000147         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCWV010000147 DACCWV010000000
VERSION     DACCWV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.149"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..1170)
                     /locus_tag="IVW84_002508"
     CDS             complement(76..1170)
                     /locus_tag="IVW84_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7918676.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IVW84_002509"
     CDS             1359..2120
                     /locus_tag="IVW84_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7918677.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVW84_002510"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVW84_002510"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7918678.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IVW84_002511"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IVW84_002511"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7918679.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IVW84_002512"
     CDS             complement(3760..4773)
                     /locus_tag="IVW84_002512"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7918680.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IVW84_002513"
     CDS             4938..5858
                     /locus_tag="IVW84_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7918681.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACCWV010000148         5840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCWV010000148 DACCWV010000000
VERSION     DACCWV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.150"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(66..524)
                     /locus_tag="IVW84_002514"
     CDS             complement(66..524)
                     /locus_tag="IVW84_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7918682.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(537..1862)
                     /locus_tag="IVW84_002515"
     CDS             complement(537..1862)
                     /locus_tag="IVW84_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7918683.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1879..3291)
                     /locus_tag="IVW84_002516"
     CDS             complement(1879..3291)
                     /locus_tag="IVW84_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918684.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3308..4072)
                     /locus_tag="IVW84_002517"
     CDS             complement(3308..4072)
                     /locus_tag="IVW84_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7918685.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4176..5051)
                     /locus_tag="IVW84_002518"
     CDS             complement(4176..5051)
                     /locus_tag="IVW84_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918686.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5306..5390)
                     /locus_tag="IVW84_002519"
     tRNA            complement(5306..5390)
                     /locus_tag="IVW84_002519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5354..5356),aa:Leu,seq:caa)
     gene            complement(5408..5479)
                     /locus_tag="IVW84_002520"
     tRNA            complement(5408..5479)
                     /locus_tag="IVW84_002520"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5445..5447),aa:Gln,seq:ttg)
     gene            complement(5489..5561)
                     /locus_tag="IVW84_002521"
     tRNA            complement(5489..5561)
                     /locus_tag="IVW84_002521"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5526..5528),aa:His,seq:gtg)
     gene            complement(5623..5707)
                     /locus_tag="IVW84_002522"
     tRNA            complement(5623..5707)
                     /locus_tag="IVW84_002522"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5671..5673),aa:Leu,seq:caa)
     gene            complement(5728..5798)
                     /locus_tag="IVW84_002523"
     tRNA            complement(5728..5798)
                     /locus_tag="IVW84_002523"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5764..5766),aa:Cys,seq:gca)
BASE COUNT         1774 a         1249 c          892 g         1925 t
ORIGIN      
        1 catttcaaaa aaattttgta aattcaccca taaatatcac cctatttaac ttttttcatc
       61 gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg ttttaataaa
      121 tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg tgcttgctcc
      181 aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg gttactttca
      241 acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat atggatcagt
      301 atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg
      361 attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc gtatccaact
      421 cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat aatttgacca
      481 ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta tcacctctac
      541 tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat tttttttgcg
      601 actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa
      661 ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc catcaatcct
      721 ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt tgcaccaaca
      781 tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc ttgattagga
      841 acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat atcgacgata
      901 tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc gcggcgtatt
      961 ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt agcacgtgta
     1021 tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc taagtaatta
     1081 ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa ggttttttgc
     1141 caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat tagtttttca
     1201 ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt ataccagcca
     1261 aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc gtaattcact
     1321 gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt tacattcgga
     1381 tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt caaaatccat
     1441 gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt gattgagcgc
     1501 atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc atgtttaagt
     1561 ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca
     1621 aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg aatatttgaa
     1681 aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt tctttcttca
     1741 tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt caaaatggct
     1801 tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat ctgttttttc
     1861 atcatcatct tttcctttct attcttctaa taatttattg aattctgcca taacataagt
     1921 cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga tcccgcttat
     1981 ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct ctacccgtag
     2041 gcgtgtatta caactataga catccaggat attggctttt cctccaaacg cttgtagcat
     2101 ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt cttctaaggt
     2161 tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt ttctcttatt
     2221 gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat
     2281 caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac atgctccgaa
     2341 tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac cggcaaaaag
     2401 tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa caggctcagt
     2461 gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga ccatctcttt
     2521 attttcttta taagctgctt tatagatagc tagacatata gctggtatac caaacatcat
     2581 tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga taaagcgtgt
     2641 catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat cgtaataaat
     2701 atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca aaaacataaa
     2761 tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat caatcccaat
     2821 ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta gaatcatcac
     2881 catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta atttttgttc
     2941 tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac caaaaatccc
     3001 catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa tcgctaaaaa
     3061 accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg caactcctat
     3121 agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa ttactgggat
     3181 gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca tcgctgcaac
     3241 tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca tttttattcc
     3301 tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt ttcacttttt
     3361 gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac acaaaacttg
     3421 aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg atcgttttat
     3481 cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta ttgatttgtc
     3541 gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa tcgacatgac
     3601 gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt tttgctaatg
     3661 taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg tctaaatgag
     3721 ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc tgatacagtt
     3781 gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac tctccgcttt
     3841 cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc
     3901 ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa cccggcatat
     3961 ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata atcccataat
     4021 tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc atttcattct
     4081 ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt actatgtgtc
     4141 atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc tagatgaatt
     4201 gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc gatgatatct
     4261 ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt aaaaaaacca
     4321 atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa tttgatatga
     4381 accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata ggatttttct
     4441 agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa actattaggc
     4501 tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt tgaccctaac
     4561 tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc atattcattt
     4621 ggataaagga atctcatttc tggcaacata attgctgtga catctctatc ttctcttttt
     4681 cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga atctaatgga
     4741 gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat aaattcgatt
     4801 ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc taatacaaat
     4861 aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa gccaatccct
     4921 ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt atgattcagc
     4981 tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa acaacttgca
     5041 gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa cgacctaaga
     5101 aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa tttacaaaaa
     5161 tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta tgataatact
     5221 tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc aactctctat
     5281 aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa
     5341 cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa
     5401 ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
     5461 accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc
     5521 ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt
     5581 taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg
     5641 aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca
     5701 cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg
     5761 ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta tcaatctata
     5821 aaataaagaa taaattatag
//
LOCUS       DACCWV010000149         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCWV010000149 DACCWV010000000
VERSION     DACCWV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.151"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(90..569)
                     /gene="rlmH"
                     /locus_tag="IVW84_002524"
     CDS             complement(90..569)
                     /gene="rlmH"
                     /locus_tag="IVW84_002524"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7918687.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            997..2286
                     /locus_tag="IVW84_002525"
     CDS             997..2286
                     /locus_tag="IVW84_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7918688.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2325..2930)
                     /locus_tag="IVW84_002526"
     CDS             complement(2325..2930)
                     /locus_tag="IVW84_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7918689.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3135..3599)
                     /locus_tag="IVW84_002527"
     CDS             complement(3135..3599)
                     /locus_tag="IVW84_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7918690.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3621..3941)
                     /locus_tag="IVW84_002528"
     CDS             complement(3621..3941)
                     /locus_tag="IVW84_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7918691.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4085..5164)
                     /gene="pepA"
                     /locus_tag="IVW84_002529"
     CDS             complement(4085..5164)
                     /gene="pepA"
                     /locus_tag="IVW84_002529"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7918692.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5364..5714
                     /locus_tag="IVW84_002530"
     CDS             5364..5714
                     /locus_tag="IVW84_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7918693.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
BASE COUNT         1700 a         1167 c         1083 g         1859 t
ORIGIN      
        1 ttctcaacga ttttcaagtt aactttttta tatataaatt tcaatgaaaa gctaacttag
       61 tgttcaggag cattcaatct taaatatcat tatttatgat acggagcccc cgcattgatc
      121 cggaaggcac ggtagatttg ctcgacaagg atcagacgca tcaattgatg tggatacgtc
      181 atttttccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
      241 gagccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
      301 ttagcaaaag attcacttgt aggatttttc ccttcaattg ctaaggcata gacgtattct
      361 tgttctttga ttttcgctaa gatccgttcc ccttcttttt cttttacttg tctcatttgc
      421 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gatttttgca
      481 taggcattca gccgtttgac atactcattg ataccttgga tcaggtattt ttcttttaat
      541 tttcctactg agataatttt gatattcatg ttttctctcc tttggtcttc atgctcactt
      601 tgtttattat aatcaatttt ttcgtctata tgtttatttt ggctattttt gttaaaaaga
      661 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag tgttatttta
      721 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
      781 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
      841 agtatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
      901 tttttcataa aaagttcaca gtctcaaagt agactaaagt caacaaataa gactatacta
      961 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     1021 aagatgaaaa aaagtaaaaa taatagcctc tggcgtaagt taggtctggg gcttgttgga
     1081 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     1141 aatagttcta ctgctacaag cggcaatcag aattctgccg gtgaaacagt tgtggaaaat
     1201 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     1261 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg actattcggg
     1321 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     1381 ggcgtgatct acaaaaaaga cgggaatact gcttatgtag tcacgaataa tcacgtagta
     1441 gatggccagc aaggtcttga agtcttgatg aaagacggta cgaaggtaaa agctgaatta
     1501 gttggaacag atgcttattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     1561 acagttgcct cttttggtga ctcaagtgct ctgaaggttg gtgagcctgc gattgcgatt
     1621 ggttccccac taggatctga atatgcaaat tctgtcactt caggaatcat ttcttccttg
     1681 aaccgacaag tgacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     1741 acagatgcag caatcaaccc tggaaattct ggtggtcctt tagttaatat cgaaggacaa
     1801 gttatcggta tcaactcaag taagatcgca agtacttctg aatctacttc taatgtaagc
     1861 gtagaaggaa tgggatttgc tattccaagt aacgatgtag tgaatatcat caatcaactg
     1921 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     1981 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     2041 atcgtgacaa atgtccaaac agctacaccg gctgaaaaag ctggattgaa acaatacgac
     2101 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     2161 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     2221 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     2281 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatgct
     2341 tctccgacta cggcatcctc aggcaatact aagattcctt tttttgctgg agcatttggc
     2401 aaatgcaatt cacgagcgga acagatcatt ccatagctat ctacccctcg caaagttcct
     2461 ggccagatca tcattccatc tggcatcata gctcctggtt ttgcaacgac aactttttgt
     2521 ccagcttcga tattgggtgc accgcaaacg atttgcaata ctgtaccatt gtctacttct
     2581 gtctcagtaa tcgataaatg gtcagaatca ggatgtggga cgcatgtttt tacgtacccg
     2641 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttgatc
     2701 aatcgattca atttcgctaa ttcctcatct gttaagctta cttggccgtt cccttcgatt
     2761 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     2821 atccgagcaa cattccattt gcgttcaaca gtgttttctg ttcctttatc gtcggctata
     2881 ataaccatca aggtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     2941 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgaaattttt
     3001 ttcatgcttt atttccgaag aagcctcttc tggaacacca tttattcgta acaagggact
     3061 gtgacaagac ttttgtcaca gtccctttac tgttttagtt aattgattaa aaaggtcgta
     3121 caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca
     3181 caaggtgaat gacgtatcac gtagctggct acacttcctg tcataaagcg ttcgactgca
     3241 tttagacctg actgcccaac catgattaga tcgatctcat atttttttgg caatttgact
     3301 gccatggatt cacgaacaga accatatgca ataattcctt ctatatttgt gtaatcaaca
     3361 gatttcccat attctttaca ttcttcgatc atttccttgg ctgtttcagt ttgctgatcg
     3421 actagatttt gatcaatagg tgcatagccc ataaagttat aaaattgctg atcgattacg
     3481 tttgctacat atactttccc attattacgg cgagccactt ctaccgcttt tttgaaagca
     3541 gcaagtgctt gttcactacc gtcgatacct actaaaatat tcttatattc ttgattcatc
     3601 ttgacccctc ctaacagatg tcagcctttt gttacacttt ctaggaacga ttcgatttcc
     3661 tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg
     3721 acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg
     3781 tctacttcaa taaattgcca atcagaaaag tcattttcaa tttcaggcat ttgcggtttg
     3841 ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactctt tccagtttct
     3901 acgtagcctg ctaattcttc taaactattt gggataatca ttctttgtcc tctactttct
     3961 gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct
     4021 ctttttataa aaaaaatctg gcaaaaagca atcttttgcc agattttata gtttcactat
     4081 cgcattattt tccgtatacg atcgtattca ctgttgattt atccaaacct ttcagtgttt
     4141 gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat
     4201 ggatataacg tcccactacg ccaatcactg tacttgggat tccttcgttt tgtgtatgag
     4261 cagcacctgc atctgtaccg ccttttgaaa caaagtattg ataagggata ttgtttgttt
     4321 cagcgatatc cagcaagtac tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga
     4381 tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat
     4441 ctgctgcaga gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt
     4501 tagaaccacg caagcctact tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta
     4561 atgtttcatt ttgcaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt
     4621 cccatgattt actgataata ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa
     4681 cgattgtgtc tcctggaagg acaccgtaac tcattgcctc ttctcttgat tcaaacccag
     4741 catcgaacaa aatatcggtt acttctactg atttttgtcc gcttgttcct cgcaataaat
     4801 gtgggggaac ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa
     4861 aacgctgagc agaaacaacg taaggattcc agccgcctag aggtacgact ttaaaaagcc
     4921 cgttttcttg gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta
     4981 cacggggtgc atccgcttct tcatgatggc gcagaccaaa aatgccgccc aaaccatcta
     5041 actgtaactc atctaccagc ggttccatat gactttccat ataagcgcga acatcttgtt
     5101 caaaaccgct tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt
     5161 ccatatgcta ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg
     5221 ataaaatatg attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag
     5281 cggttattta tttcaaagaa aatatgagag tgtggtaaaa taagcacaag gagtaacaag
     5341 tgagaagaag gaggtatgtc cacatgtatg aagaaagcga actaagctat tttaaaggcg
     5401 ggttagcagt tggaatcggt ctaggattat tgagcggggt agcttcaacg atttggtatc
     5461 atcgcaaaaa agtattgtca gcagaccaag tacttgaaaa tatcaagtca tcttttctaa
     5521 aagaaggtgc aatcgaaggt tcttggatct cttttgaaaa ggaacctatt aggaaattcg
     5581 ccgttcggtc gagtgcttat cggggaggtc tttctcgaat agaagatggc gaaactgttt
     5641 attatgaatt tttagcagat gctcatacag gaaccgtatt agaaatttca agaaaaaaag
     5701 gtgcaacaga ttaatacgtc aaaaaagtga gagaacgaat acgcacccac tattcaaaag
     5761 agaggtatca tgtccttctt ttgtagtggg tggtttttta gatagagct
//
LOCUS       DACCWV010000150         5788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCWV010000150 DACCWV010000000
VERSION     DACCWV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.152"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            33..1493
                     /locus_tag="IVW84_002531"
     CDS             33..1493
                     /locus_tag="IVW84_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918694.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1714..3477
                     /locus_tag="IVW84_002532"
     CDS             1714..3477
                     /locus_tag="IVW84_002532"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7918695.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3561..4019
                     /locus_tag="IVW84_002533"
     CDS             3561..4019
                     /locus_tag="IVW84_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7918696.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            4128..4811
                     /locus_tag="IVW84_002534"
     CDS             4128..4811
                     /locus_tag="IVW84_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7918697.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4958..5572
                     /locus_tag="IVW84_002535"
     CDS             4958..5572
                     /locus_tag="IVW84_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7918698.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5588..>5788
                     /locus_tag="IVW84_002536"
     CDS             5588..>5788
                     /locus_tag="IVW84_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918699.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFN"
BASE COUNT         2059 a          906 c         1180 g         1643 t
ORIGIN      
        1 atcataagaa aaactggagg cgtgtatcag gaatgctgga aaattacatc gagcgaaata
       61 tatttagaaa agtgtatctg tgtgaacaat tatttgaatt ccaagaaata gacatcgaac
      121 aaacagctat ttctctaagg gtgactacac caacgatcct tcacgatcta gaaagtctag
      181 ccgagtgtct agaatattgt ataaaagaac aagtaagaga gaagcataag tataaactcg
      241 tattcaaaca tggtattgct ctttctgagc tcacacagtt tctgtacggt caatcttatt
      301 ttttaaagtt tttaagttat tattttaatg gtatattcac gagtacagaa ctggctgatc
      361 ttgaatttat ttctttgagc aaagtatata caatcaaaaa aatcgtattg gattttttta
      421 aagaaaatga ttatttgaaa aataaagaaa tcattatccc agaatttgac tcgcggaaca
      481 tcttgctagc tttagtacgt tatgtcaatt gggaaggcta tgaaaataaa aatccgtatg
      541 tagaagaagc ttgtaacgag ttgattgatt atgtagagac tcattttttt aaaagaagat
      601 attcggaaga tgaaaagtcc tttatcatac gaggaataga gatcgcaatc ggaagaaagg
      661 catttcccct tggactgaaa gaagaagata agaaaagcgc agaaacgaag ccgttatttc
      721 aattagtctg tcaaggattg gaagagcaaa aaggaattct agacttacaa gaagaagatg
      781 tctattatat tttttctttg ttcaacagta gaaattatac caatgagaac atggagctgt
      841 taagaaaaga tttcgaagtt gtttataaaa attttgttct caatgaccta tcactgaatg
      901 aattgatttt acagattatt agtatcgctg aaatcgacga tgcagaaaat tttattttca
      961 agcaatcctt tttacaattt gtccgtacgt tgtgggcaga tggacaagta tttttaccgg
     1021 aaaggatcta tcttctatca gaaaaagaac aacttctgta cggaaaaatt gtagaaatat
     1081 tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat ttcattcgaa
     1141 aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc ggggagacag gaaatagaaa
     1201 tttttgttgt ttcagaaagt tcagtcaaac aattttttta tcgaacccaa tttcatttgt
     1261 atgcggaaga aggcatcgct gaaataaatc ctattttatt tcgttcatta gaagaattgc
     1321 ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct agctatcaaa
     1381 atggattaga aactgaaaag acaaagatct atccaatctc attaaaaggt gcaaacatcc
     1441 atttgtatca tattgctcag gaacttcacc tattaaaaaa acatatttct tgatgagacc
     1501 ttaattagga gaccttctac aaagcttttt aattgtctgt agaaggtttt tatttgagtc
     1561 ctgattcgtg cgaagatcta cttatataac aagctttctc tgaaattttt ttaaaatgct
     1621 attgacttag agtcaactct aagtagtaga atagataaag tgtaacaaat ctattttcat
     1681 taaaagaaga tagtacggag gagagaatgt attatgagta cagtattaca tgtattggat
     1741 gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa acagttggca
     1801 gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg taggtgggca cgttggtccg
     1861 aatctaggtg tagtagaatt aacgattgcg tttcattatg tatttgattc accaaaggat
     1921 aaaatcgttt gggatgtttc ccatcagtct tatggtcata agcttttaac tggtcgaaaa
     1981 aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat attccaacca acatgaaagt
     2041 aaacatgact attttacagt ggggcacaca tctacatcta tcagcttagc aacaggttta
     2101 gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg tagcatttat cggtgacggc
     2161 tccctaagtg gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt acaatccaat
     2221 ctaatcgtat tagtaaacga taatgagatg tctattgatg agaatcatgg cagtttatat
     2281 aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct atttaaagca
     2341 atgggatttg attatcgtta cgtggataat ggaaacgatc tggaaacggt gatccgttta
     2401 tttgaagaag taaaagatat tgatcatccg attgtacttc acgtccatac ggaaaaaggt
     2461 cgcggttatc agccggcaat cgataataaa atgaaatatc attggcatgt accatttgat
     2521 ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa catttaatat gatcatcatg
     2581 gattatttgg ataagaaaat gcaaacaagt acagctccta ttgccgcagt caatgcagcg
     2641 ataccaggcg cctttggact gaaaacattc caagaaaaac atccagatca ttattttgac
     2701 gtggggatcg ctgaacagca ctctatcact ttcgcttctg ctttggcttc tcagggagta
     2761 cgtccagtcg tcttccaatc aagtactttc ttgcaacggg catatgatca attatcacat
     2821 gatttagcga tcaacgagaa tccagtgatt attttagtcg gtggcggaac gatctcagct
     2881 agtgaccgaa cacaccaagg aacatttgac attccttact tatcaagtat cccgaacatc
     2941 aaatatcttg ctccagcaac aaaagaggat atgatctcta tgctagattg ggcgttcgaa
     3001 caaacagcgc agcctgtagc aatccgaatt cctacacatg gtgtagaaca tggtacagct
     3061 atcgcaaatg attatagtat tccagcctat caaacagtaa ataatggagg aaaagtagca
     3121 attttagctt taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc ttttagaaaa
     3181 aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca cagaaaatga cgtagaaaca
     3241 ttagataaat taaaagaaac acacgaatta gtcatcacat tagaagatgg tagtttgtca
     3301 ggaggatttg gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa cgtattaaat
     3361 tttggtgcag agaaagaatt cacggatagc gttcctgtag ggcagcttta tgaacgttat
     3421 catttaacac caaaacaaat cgttgaagat agtctgcgtg tcttgaatat tcaataattc
     3481 attatgctca ccatccagat aattgtttat actagtatct ggatggtttt ttcattataa
     3541 aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga atatgtcaaa aagttccaag
     3601 aaaaatatcc tagttatcaa tcagaaaaat taggtttctt ttcatttggg gatacagaaa
     3661 aaatggcaga cgagttgagc gaactagttg taaaaaagat aaagactggt acatcaagtg
     3721 gttacgatct gtataaacaa gagaatgagc ctttaccaaa agttggtcaa ttagacgtgg
     3781 tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa tataaatgtg accatcttgc
     3841 cttttagtga agtcacgaaa gagcatgctt ggaaagaagg agaaggggat cggacattag
     3901 cttattggcg gaaagtccat gctgagtttt ttaaacatgc ttataaagaa gcgttgaaca
     3961 tagaatttac agaagagaag ctggtcgtat acgaagaatt tgaagtaatt ttcttttagt
     4021 aagaaaggtc tgctgaaaga aatagctcat tattgtctcc tgaatagaaa agggttacat
     4081 ttttatatga agctgtattt ttatataaca ttactggagg agatattgtg gatacactgg
     4141 aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt ggaaaacgag ccgctacaca
     4201 gttcttttat tatggggaga atggctttgg ctgcaaaaga aggcggagca gttggtattc
     4261 gagcaaatac gacagaagat atcaatgaaa tcaagcgaat cgttgattta ccggtaatag
     4321 ggattatcaa aagagattac gatgggtcag aagtgtttat tactgcgaca gaaaaagaag
     4381 tggaagaact tttggagacg caagcagaaa tcattgcact ggatgcaact tctcgtaaac
     4441 gtccgacaga aaaaacaact gctgaacttg tccaaatgat tcacgaacac ggtcggttag
     4501 ctatggcaga tatttcgact tttgaggaag cgcttcaagc gcaagaagat ggttttgatc
     4561 ttttgtctac gactttagca ggctatacag aatattcacg aaagacgcag acacctgatt
     4621 ttgatttatt gaatcaaatc atagaacaag tcgatatccc agtaattatg gaaggacata
     4681 ctgactctcc tgaacaagta gagaaagcat atgaattagg tgctttttca gtcgtagttg
     4741 gatctattat cacccgtccg caattgatca ctaaaagata tgtcgaagcc gcaagtaaag
     4801 caaataacta aaaaacacgc acggcacaga ttcttgacaa aaaagaaaaa ttgatagata
     4861 gtttaaagca gattctaaga agatgaaaaa agcattactt ttcattttgt cttcaagatc
     4921 tgctttttaa cgaattttat agaaagaagg cgaaaccatg tttgaacgga gtccttcaga
     4981 attgacagaa cgtgaaaatt acaagttgct gattggttca gtcatcccta gaccagtagc
     5041 agtagtaacg actaaatcag caaaagatat agtgaatatc gcacccttca gttacttcaa
     5101 tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc cagagaaaac aagatgcagt
     5161 aaaagacaca gcaaagaata ttcttgagac aagagaagct gttatccata tccttgatga
     5221 ggacaacgta gaagaagtaa ataaaacagc agccaatctg cctaatgagg aaagcgaact
     5281 gtacctgact gacttgactt taactgattc agtaatcatt tcagtaccag cattgacaga
     5341 agcaaaagtt cgttttgaaa catccttata tgaacatgtc gagatacgga ataaaaataa
     5401 agtaacagct gatttatttt tgctagaagt acagaaatat catttaaatg aaacagtcta
     5461 tgatgataag acaggaagga tcgatccaag aaaattgaat gctgtaagcc gtttggcagg
     5521 aaatgattat gcagcaatag gagaaatttt cacgattgca cgtcccgaat aaaaaggagc
     5581 actagttatg ttaaaaataa gctataaacc atcgacagat tcaaaagaaa tgaaaaaaga
     5641 atatgaaaca gtgaatgatt ttctgcaagg gcagtactta gaagttccgc cattgcaaga
     5701 ccattttgtc gttactactg ttactttaga tggtaaggaa atagaaatgc cagatcaaac
     5761 gattagcgga ttgtttaatt actttaac
//
LOCUS       DACCWV010000151         5781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCWV010000151 DACCWV010000000
VERSION     DACCWV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.153"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            83..979
                     /locus_tag="IVW84_002537"
     CDS             83..979
                     /locus_tag="IVW84_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7918700.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1129..2070)
                     /locus_tag="IVW84_002538"
     CDS             complement(1129..2070)
                     /locus_tag="IVW84_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7918701.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2196..3317
                     /locus_tag="IVW84_002539"
     CDS             2196..3317
                     /locus_tag="IVW84_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7918702.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3575..5077
                     /locus_tag="IVW84_002540"
     CDS             3575..5077
                     /locus_tag="IVW84_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7918703.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5144..5671
                     /locus_tag="IVW84_002541"
     CDS             5144..5671
                     /locus_tag="IVW84_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7918704.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1890 a         1025 c         1220 g         1646 t
ORIGIN      
        1 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
       61 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
      121 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
      181 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
      241 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
      301 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
      361 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
      421 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
      481 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
      541 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
      601 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
      661 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
      721 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
      781 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
      841 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
      901 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
      961 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     1021 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     1081 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     1141 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     1201 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     1261 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     1321 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     1381 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     1441 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     1501 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     1561 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     1621 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     1681 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     1741 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     1801 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     1861 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     1921 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     1981 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     2041 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     2101 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     2161 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     2221 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     2281 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     2341 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     2401 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     2461 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     2521 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     2581 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     2641 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     2701 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     2761 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     2821 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     2881 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     2941 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     3001 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     3061 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     3121 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     3181 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     3241 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     3301 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     3361 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     3421 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     3481 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     3541 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     3601 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     3661 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     3721 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     3781 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     3841 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     3901 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     3961 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     4021 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     4081 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     4141 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     4201 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     4261 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     4321 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     4381 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     4441 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     4501 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     4561 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     4621 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     4681 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     4741 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     4801 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     4861 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     4921 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     4981 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     5041 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     5101 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     5161 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     5221 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     5281 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     5341 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     5401 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     5461 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     5521 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     5581 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     5641 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     5701 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     5761 atataaactc aacctttaga a
//
LOCUS       DACCWV010000152         5772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCWV010000152 DACCWV010000000
VERSION     DACCWV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.154"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            14..1168
                     /locus_tag="IVW84_002542"
     CDS             14..1168
                     /locus_tag="IVW84_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7918705.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1162..1584
                     /locus_tag="IVW84_002543"
     CDS             1162..1584
                     /locus_tag="IVW84_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918706.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2718..3686
                     /locus_tag="IVW84_002544"
     CDS             2718..3686
                     /locus_tag="IVW84_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7918707.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3696..4184
                     /locus_tag="IVW84_002545"
     CDS             3696..4184
                     /locus_tag="IVW84_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7918708.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4480..4761
                     /locus_tag="IVW84_002546"
     CDS             4480..4761
                     /locus_tag="IVW84_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918709.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4763..5179
                     /locus_tag="IVW84_002547"
     CDS             4763..5179
                     /locus_tag="IVW84_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918710.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2148 a          985 c          942 g         1697 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga atcttttagg ggtctttttg tctgttttta ggggtctttt
     2581 tgtctgtttt taggggtctt tttgtctgtt tttaggggtc tttttgtctg tttttagggg
     2641 tctttaatta gggggttgac ccccattaga aagctcttta taattttgtt aagtagttaa
     2701 tcttgaagga ggattttatg gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga
     2761 ttcctttacg caaatttacg tcagtagaaa tgaatttatt cttctctata atatctagaa
     2821 tgcgtgatgt aggcgataaa aaagtacaat ttacattcga acaattaaaa gatttaagca
     2881 attataaagc aactgcaaat gttcgattca tagatgattt agaaacaacg tatgacaaat
     2941 taatggattt acgttttggt agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt
     3001 tatttaatca atttaaaatc gatggaaaag ctgatattcc ttttgctgaa atacaagtcc
     3061 atgagaaagc cttaccctta cttaataatc ttgaagaatg ggttcgatat tctctccaac
     3121 aattcaatga attagaaagc agttattcca aaaccatgtt tagattatta aaacaataca
     3181 gaaccaaagg aattgccaat ttttcaaaag aagactttca tgaattactt gatatcccta
     3241 aaagttatag agaaagtgat ataaataaaa aaattataaa gcctattcgc caagaactaa
     3301 ccgcaatctt caaaggtttg acaatccaaa agagatacgg aaaaggacgg ggaaagcctg
     3361 tcattggcta tcaatttaca tttaaacctg agataaaaaa atcagacgac tttaacaaac
     3421 atgctccaac caaagaagaa caaaataaag ctttggaact agctaatcaa caggttcaat
     3481 ctgaaaaaga acctacttca ttacaagaaa aaatcaaaga acaaaaacga aaattagaac
     3541 ttttacaaca gttagcagaa ttagaaagaa aacaaaatac cccaagtaag aaaattaaca
     3601 aacaaatagc agataacaat tccgatatta ccccagaatt taaagaaaac ttactagata
     3661 atctccaaaa cttatttaaa gaataagggg tttttatgac agataaaact atcaaagaat
     3721 tagcagatat tctaggtgtt tcaaaagata aagtgaagta tcaactgagg aaattaccta
     3781 gtaattttac ctacaaaaaa ggaaatatta cctacctgaa aaaagaagca gtaacaacaa
     3841 ttacaaaggt tttatcaggt aatgaatcgg gtaacttacc tagtgaatta cccgattcat
     3901 tacctaccga ttacctactc aaggagattg aaactaagaa taatcatatt gaagaaaaag
     3961 ataggcaaat tgaaaaactt caaatagctt tagaaacgca gcaaaaatta ctcgaccagc
     4021 aacaacaact caccctacaa gccaatcaac aaatcaaaaa acttcacgaa caactccagc
     4081 taacctatgc agaagaatct tcagaaaaag aacccctttc tgttactgaa gaaactcaaa
     4141 aaggacaaaa caaggagaaa aaatggtggc aattctggca ataacccaac aatttttatt
     4201 cataaaaatt gttgggttac tttctttttt tgcttttaca agcatagcga catgatctca
     4261 cgtcgctatt ttttaaagag cataaggaaa cgcggtatca ctttcttacg aaaggtgcta
     4321 ggtaggctgt gcctaccacg tttctaagaa aaaaatttgc tatcactttt tcgtcattgt
     4381 ggatagctta tgtatagtgt ttcggcaaaa aacagctata caaatactat atttgtatga
     4441 tatacataaa ttatacacaa atgcgaaagg aattcttcta tggacatttt agttcggaat
     4501 attgatccaa aagttatttc agaaatagat agacgttgcc aagaactcac aacaaaaact
     4561 ggcaaaaaat ggagccgtaa tgcgtaccta aaagttctaa tcgaaaacga ttttgaccag
     4621 gcacttaggc attacaaaaa agatcaattc gatttattac tcgaaaaatt tatcgatata
     4681 caagcaaaaa atactgaaac attgaacgac tatgttcata caaacaacca actcattgaa
     4741 acacttatcg gaggaatata acatgagaaa aacttctgtt caaaaacacg ttacccttta
     4801 tcccagtcac attgacgttt tagattccat catcgaaaaa aatccagata tcaataacta
     4861 ctcggaagcc attcgttttc tatgtatgag ttatgaacct aatactaata cgacaactac
     4921 cagcaaccaa gcaaaactaa atgcgatggg aaaagaaatt tctattatgt ccgaaatcat
     4981 tgcagaattt gctgatttat atctaaaaga tactggtgtt cttatcggta caaattctaa
     5041 agtatatcaa gaagtgaaag aaaaaattga aaatcaactc aatccctccc gacgaaatca
     5101 acccaaaaat cgtcaaaaag aaaccaatca aacattcact tctagaaatt ttgacccaac
     5161 aaactttcta aaaagatgaa aaagaaaccg aaaatttttt ctatttctca gtttcttttt
     5221 tagggggcag tcttccagca agatttgcaa gttaccggta acttgcacaa aaaaagaaaa
     5281 atccgcttgt gcgaatcctt ctttttgcat tttggggcgc tgccccaatc tccaaatatc
     5341 ggttatttaa tagcgtggta actcaaaaaa atagaacgta ctctgaaata aaaaaagact
     5401 gctcttccga gcagccaata aaaaataatg agcgtttatt cttgaaaaag caacgcgctt
     5461 atgaagcgct gccaaaaatt taccatattt tatgaaaaat aacaagtttc taaaaaggaa
     5521 tcggttaaga ttcacaaaga atcgtaaaca gacaatagcg gaaaatcaag cttccgaacg
     5581 taccttttag ctagaccaat gaaaaatcac aaagtgagcc cctgtatcgg ttgatacaag
     5641 ggctacttgg catactcgac attatgtcta ctagctcgat aaataacgat tatgttccca
     5701 aaacaatatt ttatgaacta taacaaaaat ttgttatcgc atattgtata ttttaattat
     5761 aggtgtatat ca
//
LOCUS       DACCWV010000153         5768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCWV010000153 DACCWV010000000
VERSION     DACCWV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.155"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(93..764)
                     /locus_tag="IVW84_002548"
     CDS             complement(93..764)
                     /locus_tag="IVW84_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918711.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(757..1251)
                     /locus_tag="IVW84_002549"
     CDS             complement(757..1251)
                     /locus_tag="IVW84_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7918712.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1271..2491)
                     /locus_tag="IVW84_002550"
     CDS             complement(1271..2491)
                     /locus_tag="IVW84_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918713.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2638..4473
                     /gene="lepA"
                     /locus_tag="IVW84_002551"
     CDS             2638..4473
                     /gene="lepA"
                     /locus_tag="IVW84_002551"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7918714.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4567..5694)
                     /locus_tag="IVW84_002552"
     CDS             complement(4567..5694)
                     /locus_tag="IVW84_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7918715.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1769 a         1158 c         1093 g         1748 t
ORIGIN      
        1 ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt
       61 gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta
      121 taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag
      181 gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg
      241 acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg
      301 cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat
      361 aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc
      421 aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa
      481 aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa
      541 aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact
      601 tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga
      661 gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca
      721 aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc
      781 taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa
      841 tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg
      901 tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc
      961 gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat
     1021 cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc
     1081 aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg
     1141 aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt
     1201 atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc
     1261 atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta
     1321 gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac
     1381 ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta
     1441 taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat
     1501 tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa
     1561 acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga
     1621 tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat
     1681 ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc
     1741 gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt
     1801 agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat
     1861 cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat
     1921 ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc
     1981 gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg
     2041 aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat
     2101 acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat
     2161 cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca
     2221 ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca
     2281 gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac
     2341 ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga
     2401 acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt
     2461 caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa
     2521 cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa
     2581 ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg
     2641 gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat
     2701 atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg
     2761 tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc
     2821 acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc
     2881 tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt
     2941 gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca
     3001 ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa
     3061 atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga
     3121 atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt
     3181 ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa
     3241 gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc
     3301 acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt
     3361 gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc
     3421 ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat
     3481 acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat
     3541 ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa
     3601 gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa
     3661 gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa
     3721 gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac
     3781 catgttaata aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa
     3841 cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat
     3901 gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg
     3961 gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc
     4021 tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa
     4081 atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa
     4141 gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc
     4201 gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct
     4261 attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct
     4321 aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg
     4381 aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt
     4441 ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa acagtgtaaa
     4501 aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtggttt ttttcggtgg
     4561 caaaaatcaa gaattctttc taagattttc catgatatcg accgtttcat ttctcatctt
     4621 atctgttaca tgcgagtaag tatccatcgt aattgaaatt ctgctgtggc ccaatcgttc
     4681 agagatttct ttcatttttg cgccattttc gagaagaagt gtagcatgag tatgtctgag
     4741 agaatggaag ttaaaagaga gggagagtgc attcgatatc ctcctagtat tccatttcac
     4801 tacacttggc gtaactagct caccgtcttc cttcgtacat actgcatttg aatcaatgta
     4861 gagctttcca tacttcattc gattttctaa ttgttgtttc ttatgttttt tcagaattgc
     4921 tagcaaggtt tgtccaataa aaatcgttcg attggaactg cttgtctttg gcgtaccata
     4981 tacccatgcg ccgtcattct ttaccatttg tttctctaca gtaattgttc catttgaaaa
     5041 atcgacatca tcccacgtca gaccacaaac ttcgccaacg cgcattcccg tataaaatcc
     5101 aatattcaaa ggaatataga aaggatgtcc ttcaggagta atttctagca tatgatcaaa
     5161 gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt ggtcgtgctt catattttgg
     5221 tatttttaca tacagcatag gattttgctt gattaatccc caaggataaa ccgccatatt
     5281 cagcgcattc ttaaggacag agtgagtaat agtcattgtt ttcttcgagt aacccttttt
     5341 aaattcagca ttgatgaaat tttgtaaaag agcaggggag agatccgtaa gttttttctt
     5401 tcctaaataa ccgtttatat gatttttgat ggtaaatcgg tagttttcat aggtattgta
     5461 ttttagattt agtttaacgt attcctccat ccaaaaatca aggtattgtt ttactcgagt
     5521 atccgtacct aaaaagtatt gtcctgtttc gtcaatatct gataaaactt ttcgtaaagc
     5581 agcttcggcc tctggtcggg tgtctccgcc aactttctcc actttttttc ttgagccatc
     5641 atcattgata tcttcgccgt ttgtataatt aagcgagaca aataaaaaag ccatttatgt
     5701 tacactccaa ttgtaatacc cgctaaagca tacaaggaga gtgaacataa atgacttata
     5761 cccatctt
//
LOCUS       DACCWV010000154         5728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCWV010000154 DACCWV010000000
VERSION     DACCWV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.157"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            196..921
                     /locus_tag="IVW84_002553"
     CDS             196..921
                     /locus_tag="IVW84_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918716.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IVW84_002554"
     CDS             911..1420
                     /locus_tag="IVW84_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918717.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IVW84_002555"
     CDS             complement(1490..2938)
                     /locus_tag="IVW84_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7918718.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IVW84_002556"
     CDS             complement(2938..3654)
                     /locus_tag="IVW84_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918719.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IVW84_002557"
     CDS             complement(3635..3985)
                     /locus_tag="IVW84_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7918720.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IVW84_002558"
     CDS             4129..4902
                     /locus_tag="IVW84_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7918721.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IVW84_002559"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IVW84_002559"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1663 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaa
//
LOCUS       DACCWV010000155         5667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCWV010000155 DACCWV010000000
VERSION     DACCWV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.158"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..765)
                     /locus_tag="IVW84_002560"
     CDS             complement(13..765)
                     /locus_tag="IVW84_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7918722.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="IVW84_002561"
     CDS             complement(1129..3795)
                     /locus_tag="IVW84_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7918723.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="IVW84_002562"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="IVW84_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7918724.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1641 a         1192 c          991 g         1843 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaa
//
LOCUS       DACCWV010000156         5666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCWV010000156 DACCWV010000000
VERSION     DACCWV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.159"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..454
                     /locus_tag="IVW84_002563"
     CDS             <1..454
                     /locus_tag="IVW84_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP7918725.1"
                     /translation="KELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQL
                     KANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSL
                     NDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            735..1955
                     /locus_tag="IVW84_002564"
     CDS             735..1955
                     /locus_tag="IVW84_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7918726.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1952..4672
                     /locus_tag="IVW84_002565"
     CDS             1952..4672
                     /locus_tag="IVW84_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7918727.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4656..5600
                     /locus_tag="IVW84_002566"
     CDS             4656..5600
                     /locus_tag="IVW84_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7918728.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2107 a          908 c         1113 g         1538 t
ORIGIN      
        1 caaagagcta aactatgagc ttgagtatga aaaatctatt tgttcaatcc cttataataa
       61 tattgaggaa attatagaaa aggatgctat cagcatttac aaatttttta tttttattcc
      121 taatcgtcaa ttgaaagcta atatatttaa ccatttgaaa aagatgctaa atgtgtctgt
      181 tactgaatcg gcacctgtaa atattgaaat cgtacctcaa aatgtatcta aaggtgtggt
      241 gtttgatcgt ttggaatcaa tctacaattt aaagaaacct ttgagaatcg cgatcggaga
      301 tagtttaaat gacttatcta tgtttgaaag tgcagatatt agttttgcaa tgggaaatag
      361 tcatcaagaa atcatgaaaa aagctgatta tgtcactgtt tcaaatgata aagacggagt
      421 atctcaagca tttgaaagaa ttcttaccat ataaactaat taaggtagta tagaacatag
      481 aattctaaat acggaaaaat ggagcataaa ttccagtaca aatagtactt cttcagtgtc
      541 aaacgaagcg aatataacat agatatttag gattgtgaca aaaatcatgt cacaatcttt
      601 ttttcgaata aacttatttt ttgaagtcgg ccatttatcg taatttcttt gcataatacg
      661 aacaaatttt cggttattga tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa
      721 ggaggaaatt tttaatggtg aaatttatcc atgcggcaga tttacatatg gatcggtcat
      781 ttgaaggact gacaactctt gataaaactg tacaggaaaa actattgaag acgaatctga
      841 ccgttttatc aaatatcatt gaacaggcaa taataaataa cgttgatttt gttttacttg
      901 caggagataa ttttcatcaa aatcgtcctt cattaaaaat acagaaacat ttttcagaac
      961 agatgaaacg gttagaaaag aaccatatac ctgtatttat aatattcgga aatcatgatt
     1021 tctatcaaaa agagcggtat tggttctcct ttcctaaaaa tgtccattta tttacatccg
     1081 aaacggtaga gacaaaaaaa ataacgataa aaagtggaga aactgtctct ctcagtggat
     1141 ttagttatcg tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct tctagagaac
     1201 tcacagatta ccatattggg ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact
     1261 atgctccttt ccaaccctca aagatgcaag aaaagggata cgattattgg gctttagggc
     1321 atatccatgt gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc
     1381 aaggtcatac taaaaaggaa caagcttcta catctattct actagttgaa ttaagtaaag
     1441 gaagttgcca aatacatccg attaaagtag cagaggtcta ttggaagaca aaggaaatat
     1501 cattgaggaa agcaagaacg acaaaagagg tcattgttca cattcgtcag cagctttcaa
     1561 aagtagagga tggatttagt ttaattgaga taaaattaaa agattatgac catctcaata
     1621 cagatgtttt ggaacggata caatcaggag agctattaga ctatttgtta gaggaattct
     1681 cagataggat aaatgatttc tttatttggc gtatttctct catagagaat actttttata
     1741 cgaagacccc acttgctgct tctgctaaat tgatggaaca gttatttcaa tactatcaga
     1801 cacctcaaga tttccaacaa attttaaatg aagtatatag tcatcaagaa gcggctagaa
     1861 tattgagcga actgccggaa tatcaagaag aaacacttga aaaagccaag cagctgctta
     1921 atcaagattt tttatttgag gaggatcagg aatgaagatt ttaaggatcg agattgctgc
     1981 ttttggaaaa tggcgtcaaa aaagttttga cttttattca ggcaatcagc tgatctatgg
     2041 gggaaacgaa gcaggaaaat caacgattta tcaatttatt caagcaatat tatttggttt
     2101 cccttcaaaa ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag cctatggcgg
     2161 caaaatatgg cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct acaagcagca
     2221 aaatcgcgga aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg agttactaga
     2281 aaaactgatc gcacctttga cgaaagagtt attccagcag gtatttactt tccagcaaga
     2341 gcagttgatg gaactagatc atttgagaga aaaagagcta catgatgcgc tgatttcttt
     2401 aggcatcacg ggcagttctc aagtattcca aaagcggcaa gaatattatc agaaagcaca
     2461 acaattattc aagaaaaaag gccaaaaatt actaatcaat caaaaattaa atgaatggaa
     2521 agagctgcaa gaaaaaattc atcaaaaaca agaacaggaa gcttattttg gcaagttgat
     2581 ccaacaagaa caggaatacg agtcgcaaca gcagcagtta ttggcacaat taaaagaggc
     2641 gaacaaacaa ttttcattag ccagacagca agaacttaat ttttctttat atgaagaatg
     2701 gcaatctctc aaaaaaaaca gcactacagg tcttcctgat gagcaacttc ttttagatct
     2761 ggagtctttc tccaaacggt atcagcaaac aaatgagcgt ttggaagaat tagaagcaac
     2821 actggaaaag catagcggga tggatcagca gtcagccaga tattattttt atttagagca
     2881 ggagcaggaa ctaaaagatc tccagcaact tcaatttaca gcggaacaat taaacgaaga
     2941 atggcaaaga ttacaaattg agactagaga aacaaattca gaattgaaga ttttggaaca
     3001 aaggtggcat tggcagaaaa atccgttacc ggaagaattt ttatatgaag aagaatggaa
     3061 gcatcttctg gctgaagaaa gaaggattgc tgatctagaa agccagattt ctgtgcgtct
     3121 ccaaatagct aaagaacagc aagagatgat tgaagcagaa gtgaatgact tggaagagac
     3181 ctatccagca ttattacaaa atcagtcaga gatagcacca gtctcttcat ctaaaccgta
     3241 tatgctgatg gctggcggag caataatcat tgcaggtata tttgtaccaa tgcccttgaa
     3301 agtgatcctt ttactaattg gtgcaggcat gattggctat ttcttctaca aaaggcaacc
     3361 aaaaattaaa actaaaaatg atttctctga gataaaagga atgtggcaag aaaaactagg
     3421 acaacttgat ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg
     3481 agaagaaaaa cagattcttc agcaagaagt atatcgtttt gcggatcatc ggcacttagg
     3541 aaagatggat acgctcaaac tcatgaaaga atatggacag gaagtcatag attatcagaa
     3601 attatatggc gtatttgagc ggaaacataa aagagaacag gaaatacaag aaaaactacg
     3661 acaaatcgac caaaaatttg agtttgttaa agaatggtta ccgatacaag ataagacaat
     3721 ttcagaaaaa atggtattgc tttcccgttt tatacaagag atggaacaga tgaaatttgc
     3781 cagaagctat cagcaaaata ctttattgaa gcaagaaatc aatcaattga aaaagaaaca
     3841 aaaagtatgg atacaagaat acagcgaaca attgactcaa gcaggcttat cttatccttc
     3901 ggaagtacct gtctttttac aaaaagccaa acaagccgaa cagcagatga aaagatacaa
     3961 tgaattatcg gaaatgctta ctccactatt tcctgaagag ctgacaaaag agaaattatc
     4021 tgtaagactg cgccaactga aagaaaagca atcccaactc cagcaagaag aaaaagaagc
     4081 aagtgaaaat cggcaacgac tccaacttca agtagaagag ttgcaaagag acggcacatt
     4141 ggacgaactt tatcaacaag ctagccggca aaaagcaaaa ttacaggaat tactggagga
     4201 atggggagca cttgaaatcg ccgggacatt cttaggtgat ctttcaacag agatgagtga
     4261 aagacaatta ccccagttgt taagagcagc aggtgattat ttaggtattt taacaaaagg
     4321 aaattatcag aagatattac tgaaaaatga tacactgact ttaacagacg gatatacaga
     4381 atttcccatc tatgagttat ctacaggtac gaaagatcag ctgattatgg cgatgagatt
     4441 tgcttacctc tcattagaag ctaaccgttc gatatgcccg atcatcattg atgatggctg
     4501 gcttcattat gatcatcagc gaaagtatca attagctgaa cttttagcta aatttggaga
     4561 aaagtatcaa ataatttgtt tttcttctga taaagaaatg gtaagctatt accatgagat
     4621 gaaccaaatc gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt gatttagtaa
     4681 tcgatgagca gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa
     4741 acggaaaaaa atttattgct tttactttcc aagatacttc aggtactatc gatggtaaat
     4801 actgggatgc ttcggaggag gaaatcaata aatttacagc cggcacagtc gtttttctaa
     4861 acggtaaaag agaggtttat caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa
     4921 caagggtaga tgagccagga gagcctagtt tatacatgga aagagcccct ttgaagaaag
     4981 aagaaatgga agaagaaatc aatcaattgt tgtttgagat caccaatgcg cattggaatc
     5041 gtattgttcg ctatctactt aataaatatc aaaaacaatt ttttgagttt ccggcagcaa
     5101 aaagaaacca tcatgcattt gccagtggat tagcttatca tactttgaca atgcttcact
     5161 tagggaaatc aatcgccgat gaatatcctg agctgaatcg agcattgttg tatgctggta
     5221 tcattttgca tgatttagga aaggtgattg aactttccgg tcctatgtcg acagaatata
     5281 cgcttgcagg aaatttgatc ggtcatctgg tattgattga cgaagaaata actaaagcct
     5341 gtcttgcgtt gaaaatcagc gaagctgacg aagatgttct cgtattacgt catatggttt
     5401 tagcacacca tggcttatta gaatatggct cacctgtacg tccaagaata atggaagcag
     5461 aaatattaca tcaaatcgac aatatcgatg cttcgatgca gatgatgttg acttcgattc
     5521 gtcaaacaga accaggaaaa tacaccgatc ggattttcgg tatggacaac cgaagtttct
     5581 atgtaccaaa agatttatag caaaaattta tttttttcat tcaaaacaaa aaaagcgaga
     5641 cataagtctt cgcttttttt gttttc
//
LOCUS       DACCWV010000157         5591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCWV010000157 DACCWV010000000
VERSION     DACCWV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.160"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            512..1069
                     /locus_tag="IVW84_002567"
                     /pseudo
     CDS             512..1069
                     /locus_tag="IVW84_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="IVW84_002568"
     CDS             1127..3166
                     /locus_tag="IVW84_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7918729.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGVKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="IVW84_002569"
     CDS             complement(3275..4426)
                     /locus_tag="IVW84_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7918730.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="IVW84_002570"
     CDS             complement(4580..5110)
                     /locus_tag="IVW84_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918731.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="IVW84_002571"
     CDS             complement(5209..5559)
                     /locus_tag="IVW84_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7918732.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2021 a         1029 c         1042 g         1499 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 tcaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgtgaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aataattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gaacacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt t
//
LOCUS       DACCWV010000158         5585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCWV010000158 DACCWV010000000
VERSION     DACCWV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.161"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(488..1297)
                     /locus_tag="IVW84_002572"
     CDS             complement(488..1297)
                     /locus_tag="IVW84_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7918733.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="IVW84_002573"
     CDS             complement(1297..2043)
                     /locus_tag="IVW84_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918734.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="IVW84_002574"
     CDS             complement(2061..2528)
                     /locus_tag="IVW84_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7918735.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="IVW84_002575"
     CDS             complement(2528..2938)
                     /locus_tag="IVW84_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7918736.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="IVW84_002576"
     CDS             complement(2949..3962)
                     /locus_tag="IVW84_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7918737.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="IVW84_002577"
     CDS             4179..4913
                     /locus_tag="IVW84_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7918738.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="IVW84_002578"
     CDS             4897..>5585
                     /locus_tag="IVW84_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7918739.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DACCWV010000159         5208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCWV010000159 DACCWV010000000
VERSION     DACCWV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.163"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..1368
                     /locus_tag="IVW84_002579"
     CDS             34..1368
                     /locus_tag="IVW84_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918740.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..2989
                     /locus_tag="IVW84_002580"
     CDS             1355..2989
                     /locus_tag="IVW84_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918741.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="IVW84_002581"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="IVW84_002581"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7918742.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="IVW84_002582"
     CDS             4077..5066
                     /locus_tag="IVW84_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7918743.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1826 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttta
//
LOCUS       DACCWV010000160         5191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCWV010000160 DACCWV010000000
VERSION     DACCWV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.165"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            137..748
                     /locus_tag="IVW84_002583"
     CDS             137..748
                     /locus_tag="IVW84_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7918744.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IVW84_002584"
     CDS             821..1750
                     /locus_tag="IVW84_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7918745.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IVW84_002585"
     CDS             1908..2645
                     /locus_tag="IVW84_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP7918746.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..3871)
                     /locus_tag="IVW84_002586"
     CDS             complement(2693..3871)
                     /locus_tag="IVW84_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7918747.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..3977
                     /locus_tag="IVW84_002587"
                     /pseudo
     CDS             3894..3977
                     /locus_tag="IVW84_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4098..4499
                     /locus_tag="IVW84_002588"
     CDS             4098..4499
                     /locus_tag="IVW84_002588"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918748.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5191)
                     /locus_tag="IVW84_002589"
     CDS             complement(4706..>5191)
                     /locus_tag="IVW84_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918749.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DACCWV010000161         5134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCWV010000161 DACCWV010000000
VERSION     DACCWV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.166"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..446)
                     /locus_tag="IVW84_002590"
     CDS             complement(<1..446)
                     /locus_tag="IVW84_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7918750.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
     gene            complement(753..1850)
                     /locus_tag="IVW84_002591"
     CDS             complement(753..1850)
                     /locus_tag="IVW84_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7918751.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1958..3985)
                     /locus_tag="IVW84_002592"
     CDS             complement(1958..3985)
                     /locus_tag="IVW84_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7918752.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4130..4861)
                     /locus_tag="IVW84_002593"
     CDS             complement(4130..4861)
                     /locus_tag="IVW84_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7918753.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1452 a         1042 c          811 g         1829 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa ccatcctaca
      721 ataataaaaa tttactattt gttatcttaa ccttactttt ctttctcttc tttttttctc
      781 aaaacaaaca aaagtatgca taataaaata aacaataaga aaagtgaagc tactaagata
      841 taaatccaat tattgtcttt tttaatcgta acatccgtcg tgttcaattc cttggctact
      901 tttccgtcta ttgtaaaatc tttttcaaat acccattgat tggtatagtt gatcgtagta
      961 tttccttttt ttctcgaaaa ttttccattt tcattttcat ttcctaaaac agtcattgta
     1021 aggtgatatt cacctggggt tagcggttga ccatttaaag cagttggaaa agaaaaattt
     1081 gtattcggcg caatctgcaa tccttctttt tcctcttgat ataaaacttc agaatgtcct
     1141 ttttttgtaa tttttgtttc aataactact tgattgatat aggttttttg atcattttga
     1201 acgtttgcta agatgacatt tcttgcattg atttgatctg gtttaacttc atgaagagta
     1261 aggttcggag caacttcgtt cagtgtctgt tgcatcaaca aagcaataat ataggtgtat
     1321 tcattctcaa tcgataagct ttgatcctta gtttctgttt ggtccttttc cgtctggatt
     1381 tcttggaatg tgatcccacc agccaataca ccatcaaatc gctcattcgg catcctgaca
     1441 tgaaaagcaa cggtttggac acttttaggt tttagcgtca ctatctgtgg atagctcaca
     1501 taatctacca gatcaaagat caggctcatg tctttttcaa tctcgttttt tccgtattca
     1561 accatcacat ttgaattggt ggttgcgcta ttcacagaaa tatcgatttt cacttctttt
     1621 tctgtatcat ttctcaattc cgcttttact tctacttcct gatccggatc aagttgaaga
     1681 tcgaagtaag tttttctctt gtcgatttga ttctctgaag ttatcggcgt cactgaaaaa
     1741 ttaaatgcac tagctgacac tgatatattg ttaaaaagtg cccctaaaat aactaggagt
     1801 gttataaata attgttgttt tggtctcaag aaaatcgctc ctttaatcat atgtaagaga
     1861 agactataac aaaatcttgt ctctgtggga cacaagagcc tatggtacct gtgcccttct
     1921 tgaaactcaa taattttgtt atattcttct cttcctgtta atttcctggg acatctgcaa
     1981 gtatccaaga tagtttcccc tcaaaggttc cgattctttg gttttctaca ggaatattta
     2041 ttctgattcc acgatgagct gtagaatcta aaagagagga caaatcgctt tcaccctttt
     2101 gatcactttc gaaaatcgta acagcggatg tccctataac cgttgtattt tcagaggtaa
     2161 caaaggaaag agcttctgga agctcaaaat ctccatttgt aagaactttc tcttgtttca
     2221 attgtaattt ccaacttcca aaagcccgtt catctataat tttcaaccca cctgaaaact
     2281 gtccgaattc acttaatggt ctactggata ttttatgttt tccaaaattg acatattgag
     2341 gagcagttga gagataaagt ttgccttttc tgtaataaaa atagatatcc tctgtatttt
     2401 cggagaagat gccttctgta ttcgctgaat ccgagtcaaa aacataccct tcaatatcaa
     2461 taggagtttc tttatacttc tctcctattt tcccagtgat tgttttcatt tcagcaatat
     2521 caatctgact gtctttatct ttataataaa catggatatc tatctccttc agctttgcga
     2581 tctttaggtt tatttcattc gattgctcaa tcaaattccc gttttcaagg tattcaaaat
     2641 gcatagtgag tactttttca ggaattactt cctgcaaagt tgtctccaat tcgattatta
     2701 acgtatctcc agcaataaaa taatctgtca gatttagcgt gactcgattt ccggttatca
     2761 agatatcttt ggcatcaatt tgatttccat tgatcaagat atgctcggga atagttaatt
     2821 cttgtgggag gtcgatacta gtctgcattt tcttatattc aagaaaatcc ccctcgacct
     2881 tcaatgtatt tctgatagtt atcttatcgt ttcctccagc ttcagcgatg gccccataat
     2941 cactatcgtt gacttttaca tcagctgttg atgtgacatt cgatggaagt cgcgtgacag
     3001 cgacagcatt ttcttgaaaa aaagtacctg ttgaagaagt aaacccccaa tttacttctg
     3061 tagaaccaaa ctgcctaatg agatcaggta taatccgaga agagcgaccg acaccttcca
     3121 aatcataagt aagacttctt tgctccgcgt cccatttaat tattaacaag cgccatgtgc
     3181 cattagaaag aaatgtagga gctaccgtca cgccagtatg ctctccagaa taattattat
     3241 tatttggttt aggcgtgaca aaagcgacat gtccatgccc tctattatca gcagttatct
     3301 ctcgatccat tcgatcagaa gaaccactat ttttatacgt atcaaactca atcgagagcg
     3361 cattcgcaat gtaaggttgg ccagaacgag tagaatatga gccaattccc atacctggcg
     3421 atccaataac ttgactggga ttactagcca ttctaggatc gccagtcaat gtaaaggtca
     3481 tgccatctcc cgctctatct cgttgatttc ctaagtataa ataagattta aatgtaaaat
     3541 ccgagagtaa actaatcttc ttatttgacc aaatagctcc tctttgagac ctttctgctg
     3601 ggttcaactg cagtaacttc ccattttcca atatggacgt gctagtaccg ataggtgttt
     3661 gaaaaatttt atcgatagaa ataatattgg gatcgtcagg cagctcaacc gcagaaattg
     3721 gtgcatcttc tagtccttgt ttgtccgaac cctcagcttc tacgttgtca gtggtctctc
     3781 cactactcga aatacttaaa tcctcactgc tttcttgtgt ctggttggat tctattaaac
     3841 tgtcgaatgt cgaagtgagt ttgctttctt cgtctcttct atttccatgc tccaactgat
     3901 tcaattccaa agagtaccct atgatcggaa agatattagt cactaagaga ctgcttaaaa
     3961 tgagcacgat cctcctcttt ttcaatatat cttttcctcc tgatcgttaa attcctagat
     4021 gaactattcg tatccaaaca aatttagcct agcctttgac aatttttgct cgattacctc
     4081 gcaagacaat atgactacgt atttattttt aacagttctt ttcagtaaac tagttgttag
     4141 gtgtatcttc taatgtccaa actaatttag tggaatattg ttgcgctaac ttgacagatt
     4201 taccgggtac cgaaagctgt accgcatctt gattttcatt ttcatttgca ccaaatcgat
     4261 agacccaagt ccccacacct gttcctgatt tagcattaat tgctaacgta ttgtttcctc
     4321 ccggtgtcaa cgttaattca gaattcaccg tttgcggcgt atatgttgga tcaaccaaag
     4381 aaacagcctg ccctttcgta aatttcagct gtgctccaac tagttcatcc ccactggcag
     4441 ttttaaactg ttctggttga gagacactta atttccaccc agataaggta cttctcctat
     4501 ctgtcacttg gacatatgtc ggttttctag acccatcgtt catcacatca gcagctgcat
     4561 aatacgtttt atccgcagta gaaatttttt gagtcccaaa tttgaatttt gatccataat
     4621 caatcgataa tgcccctcct gttccagggc ttggaggatc tgatggatcg acaggtttat
     4681 tgggatcagg attctctgga tcaacaggag gagttacacc ctcatctcct gcttcaaatg
     4741 taatcgttcc ttctgtgtta tattcctttg ggtatacttc agttgcagaa acaaaagtcg
     4801 tacttgctac catgctgaat aacatcattg ctgctgttaa tacgatttct tttttcatca
     4861 ttgacattcc tctttcttta tttactaaac atatcagtcg attggattaa tccatagcaa
     4921 aattctgttt tacgatactt atgaaataaa agaacataga cctatatttg gtcctatgta
     4981 tcgattagtt ataaatgatt atcagcccga tttgatttct agtttttatt gcaacttgat
     5041 gcaaacacaa tctagaaata cagctgtcat agcctttaaa ataattcgta agtccccctc
     5101 ctatctatca aaatgtgact tgactgccta gact
//
LOCUS       DACCWV010000162         5087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCWV010000162 DACCWV010000000
VERSION     DACCWV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.167"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IVW84_002594"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IVW84_002594"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP7918754.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IVW84_002595"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IVW84_002595"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP7918755.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IVW84_002596"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IVW84_002596"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP7918756.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IVW84_002597"
     CDS             complement(4028..5044)
                     /locus_tag="IVW84_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7918757.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DACCWV010000163         5044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCWV010000163 DACCWV010000000
VERSION     DACCWV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.168"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            149..808
                     /locus_tag="IVW84_002598"
     CDS             149..808
                     /locus_tag="IVW84_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7918758.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            827..2410
                     /locus_tag="IVW84_002599"
     CDS             827..2410
                     /locus_tag="IVW84_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP7918759.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2545..3123
                     /locus_tag="IVW84_002600"
     CDS             2545..3123
                     /locus_tag="IVW84_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918760.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            3069..3587
                     /locus_tag="IVW84_002601"
     CDS             3069..3587
                     /locus_tag="IVW84_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918761.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3595..4635
                     /locus_tag="IVW84_002602"
     CDS             3595..4635
                     /locus_tag="IVW84_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7918762.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            4884..>5044
                     /locus_tag="IVW84_002603"
     CDS             4884..>5044
                     /locus_tag="IVW84_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7918763.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1865 a          576 c          987 g         1616 t
ORIGIN      
        1 gttatagagg aagaaacatt atttcaatga ttaagagtcg agagcacaaa cggtagacga
       61 tgttattttt ataaaaagat ataatttatt aatggtcgat aattatgaaa ggaaaaatat
      121 tcttaaaaaa aagagtatag gatgtcgtat gaaaatagtc gctatagtac caatgaagtt
      181 aaataacaaa agattgccac aaaaaaacac gaaagctttt accaatggaa aaccattgtg
      241 ttattatata ctttcaacac ttttaaaagt aaaacagatt gataaagtgt atgtctattg
      301 tagtaaccca gatattaaag aatttatacc agaaggtgta acatttttaa agagatctag
      361 ttccttagat caggatacaa cgagtattac acaggtatta gccggtttca caaaagaagt
      421 agaggctgat gtatatgtaa tgacacacac aacagcacca tttattagcg aaaaatctat
      481 tgaaaaagga ctagatgccg tgctgacagg agaatatgat tctgcatttg ctgcaaaaaa
      541 attgcaagat ttcttatgga aagacggtga accatttaat tatgaattgg acaatattcc
      601 tagaacacag gatttgccgc ctttatacga agaaactagt ggattttata tttataatcg
      661 caatgtgatg accgagttgc atagaagaat tgggaaaaaa ccttgcatag ttgaagtagg
      721 tgagattgag tcagttgata ttgatgaagc cgaggatttt atgattgctg atgcgattta
      781 taatcatctt attttacaag gtagttaaga cagggggaag gttaggatga acaatgtaca
      841 gattttagat tgcacactta gagatggggg ctattgtaac aattgggaat ttggatttga
      901 taatacaaaa aaaattattt ctggacttat agaatcagga attgagatta tagaatgtgg
      961 gtttctcaca aataaaacag tatacaataa aaatgttaca aaatatacga ccttagaaga
     1021 attagcacag ataattcctg aaaaaagaga aggaaaatta tttgtagcaa tgatgaacta
     1081 tggcgaatat aatatagagg atcttccaga ttatgatggt tcatctattg atggaattag
     1141 gattgcgttt cataaaaaaa attgcaatga agcgttgaaa ttatgtcgtg agataaagaa
     1201 aaaaggctac ttagtttttg ttcaagctat ggtatctctc aattatacag atgaagagtt
     1261 tttagcagta ataaaaaatg tgaatcagtt taaaccatac gctttttata ttgttgatag
     1321 ttttggaatg atgaaaggaa aagatttaac tcgtcttttt tacatgattg agcataatct
     1381 agaaaaagat atacagatag gttttcactc acataataac atgcagcttg catattctaa
     1441 tgcacaaaaa cttataaccg tacaaacaaa tcgaagcctg attattgatt cttcggtgta
     1501 cggaatggga agaggagcag gtaatcttaa cacagaacta tttgtagaat atttgaatga
     1561 taatgcagga aaaaaatatc aattaaaacc attattatat ataattgatg aaatactaaa
     1621 tagtttctat cagaagagtt attggggata ttcattacca aactatattt cagcagttca
     1681 taatgctcat cctaattatg caagctatct gtcatataag aatactttaa caattgaagc
     1741 aatgaatgaa atattcgaaa tgatggatga agaaaaaaag gtatcttatg ataaacaata
     1801 tatagaagat ttatactaca aatatatgga aactgggaaa atacaggagg aatatatgga
     1861 agaactgaga aaatttctct cagacaaaaa aattctactg attgcaccag gaaaaagttc
     1921 tgtagatgag gtagaaagaa taaaagagtt tgttaccaaa gaagaagtcg taataattag
     1981 tgtaaatttt gaatattctc atatagatat agattacgta tttttaagta atttaagacg
     2041 ttttcatgaa ttaccgaaag aaagcagaac taaatgtatt gttacatcaa atatacaagc
     2101 atcggatgta tatttacgga cgagttataa aaagttgtta tctgaaaggg aagttgtaag
     2161 agacaatgca ggattaatgg caattagatt tttagccgat atgaatgtgg atgaaatatt
     2221 cttagctgga tttgatggat attcacataa tgaagatgag aattatggtg agcaatccat
     2281 ggaaattata acaaaatttg cattattaga tgcaatgaac gtgcaaatga gagatgaatt
     2341 gtcagaatta gctaaaaaag taaaggtgac atttttaacg gatccacgtt gggttcgaat
     2401 tgataagtag tagtaataat gtttttaaaa ttttgataaa gagatagtga gagaataaaa
     2461 atgggtaaag tcttaaataa aaaagaagaa aaatatttaa gggccgatgg ggcgagatga
     2521 aactctaatt ttgaactttt aagaatcatt tctatattgt gtattgttgc ttaccatgct
     2581 aatttgcgtt taaattataa tgcagacgga attatagtac atggttcatt aactgctaac
     2641 cagattttct tgaattatat tggtatttgg ggtcagctag gagttaattg ttttatattt
     2701 atttcggcat attttttaag tgagaaaaga aacattcact ttgaaaatgt tttgggaatt
     2761 gtattagaat gtatttttta tagtgtatta gtttatggaa ttctttgtgc atgtggaata
     2821 gaaacatttt cattgaacag ctttgtaaaa gaaatattat catttgctct aggtagatat
     2881 tggtttatta catcctattt agcattgtat attctaagtc cgtatttaaa tatcatgctt
     2941 aacaatctgg aaaaacttca gttgcaaaaa ataagtatgt tattgtttat aggtgtatgc
     3001 tgttatcgta tgttatttaa aacatctttg tttggggatt gtgctgtttt aattgcacta
     3061 tatgtcatat gttatacact gaaaaaaacg ataataatat ttttgagaga cacagaatca
     3121 taggaacaat tgtatgtata acagttatac cacttctcat atccatagga atacttttca
     3181 aatttatacc aaatgcaatg gttaaccaga tggttcatat tggttctcca ttgatggtta
     3241 tagcaagtat atttttattt tatgtattta aaaatttttc attttacagt aagtatataa
     3301 ataaacttgc gagaaccagt ttaggggtat atattttaca tgaaaatcat tatttcattg
     3361 atggtatgaa gtctagaggc ttaatatggg atgggatatt tggattaggc aaaaggtttt
     3421 tagataggga tttttggatt tggtttataa ttgcagttgt agttgtgtgc ataatttgca
     3481 tggctatcga tgttgtaaga gaaaaatttg aaagacagat ttttaaaatt aagagtgtaa
     3541 agaacctgtg taaacttatg aatcatgtta taaatatata cccataatta ataaatgagg
     3601 agaaaagaag tgaaagttat atcattcgga gaattattat tgagacttgc agcacccggt
     3661 tattcaaagt tatttcaaag agatagtttg gagactacat tctgcggagg ggaagctaat
     3721 gtagcggtat ccttagcaat atttggatat caatcagaat ttataacaaa gcttccggat
     3781 tcagatgttg gatatgcggc agctaatgct ttgcgttatt ttggagtaaa tgttgataat
     3841 attatatttg gagagggacg catgggactg tattatttag aaaaaggagc agctcagaga
     3901 ccatctaaag taatttatga cagaaaattt tctgcaattt cgttagctga tagtgatgaa
     3961 tttaagtgga aggagatcct aaacgatgca aattggtttc attggacagg gataaatcca
     4021 gcattgtctg agaatctctt tgagacgtcc ttagatgcat gtcaggctgc caaaaaaaga
     4081 ggtataacaa tttcttgtga tttaaattat cgatccaagc tttggacatc agaggaagca
     4141 aaagaaaaga tggaacagat actaccattg gttgatattt gtatttgtaa tgaagaagat
     4201 gcagaaaaag tgctaggaat taaagctgag catacaaata ttaatagtgg agaattaaat
     4261 aaagatggct atgtgcaggt ggctagggag attcacaaga agtttggatg taggtatgta
     4321 gctacaactc tgcgtagaag ttattcggca agtagaaatg gttggagtgc aatgttatac
     4381 gaagcagaaa aagataaagc gtatttttcc acagaataca acatacaaat tgttgatcgc
     4441 gttggtggtg gagacagctt ttctgctggt ataatatatg cgttatgtga aaatatgtcc
     4501 ggacaaaagg ctattgaatt tgcgactgca tctagttgtt taaaacatac tatagaaggt
     4561 gattttaatc gaactacaat tgaggatgta tataatttaa tgaatagcgg tggaaatggt
     4621 cgcgtgttgc gttagtcaac atataaagat aaatcacagt aatttttatg gtgattatct
     4681 gtcttagtag tgattagaat aacgtgaaaa taaattgttg agcgtttata ctgttttgaa
     4741 aaataaattt aacatattga aaaatacaaa tatatacgta tattataaag cgccgattgt
     4801 aaaattaagc tagacaacta aaacagtcat ttgtgctaca ctcaaaatag ttccgaccaa
     4861 agaattataa ggagtgagtg caaataacct atacctatct tacaacggat gaacttgtaa
     4921 taatagagtc ttatttgaaa attaaccaat ctgttgctaa aactgcgcat tgcttgaatc
     4981 gttcaagtca aacgatccat aaagtatatc tgttcttcaa gcaaagaaaa tcagccttag
     5041 aata
//
LOCUS       DACCWV010000164         5009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCWV010000164 DACCWV010000000
VERSION     DACCWV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.169"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..182)
                     /locus_tag="IVW84_002604"
     CDS             complement(<1..182)
                     /locus_tag="IVW84_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7918764.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IVW84_002605"
     CDS             517..951
                     /locus_tag="IVW84_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7918765.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IVW84_002606"
     CDS             974..1294
                     /locus_tag="IVW84_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918766.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IVW84_002607"
     CDS             1435..1626
                     /locus_tag="IVW84_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918767.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IVW84_002608"
     CDS             complement(1697..1954)
                     /locus_tag="IVW84_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7918768.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IVW84_002609"
     CDS             2112..2375
                     /locus_tag="IVW84_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918769.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IVW84_002610"
     CDS             2399..2587
                     /locus_tag="IVW84_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918770.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IVW84_002611"
     CDS             complement(2670..2855)
                     /locus_tag="IVW84_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918771.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IVW84_002612"
     CDS             3070..3441
                     /locus_tag="IVW84_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7918772.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IVW84_002613"
     tRNA            complement(3591..3663)
                     /locus_tag="IVW84_002613"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IVW84_002614"
     CDS             complement(3709..4137)
                     /locus_tag="IVW84_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918773.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IVW84_002615"
     CDS             complement(4197..4955)
                     /locus_tag="IVW84_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7918774.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1744 a          784 c          978 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttatt
//
LOCUS       DACCWV010000165         4958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCWV010000165 DACCWV010000000
VERSION     DACCWV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.170"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            114..800
                     /locus_tag="IVW84_002616"
     CDS             114..800
                     /locus_tag="IVW84_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7918775.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            802..1746
                     /locus_tag="IVW84_002617"
     CDS             802..1746
                     /locus_tag="IVW84_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7918776.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1945..2589
                     /gene="pcp"
                     /locus_tag="IVW84_002618"
     CDS             1945..2589
                     /gene="pcp"
                     /locus_tag="IVW84_002618"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7918777.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2668..3945
                     /locus_tag="IVW84_002619"
     CDS             2668..3945
                     /locus_tag="IVW84_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918778.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4203..>4958
                     /locus_tag="IVW84_002620"
     CDS             4203..>4958
                     /locus_tag="IVW84_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7918779.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1613 a          888 c         1053 g         1404 t
ORIGIN      
        1 aaaataacga aaaatattta aaaatagtgt ctatttcgtg agaaaacaac tatatttttt
       61 atgtgttttg ctttataatt aaaaaaatat tgtcatttta tggaggaaaa tatatggaaa
      121 attatcttag ccttttaggc gtcttgattg ttattctcgg gtttgcattt aaattagact
      181 cgatattgat cgtaatggtt gctttagttg ttacagcttt gactggcggt cttggcattc
      241 aaggaatgtt agaactttta ggtacaagtt ttgtaaacaa tcgcgggatg gcgattttta
      301 tcatcattat gttggcaaca ggaacgcttg aaagaaatgg tctgaaagaa tcagcggctt
      361 ccttgatcaa gcgattcaag aaagtatctg ctggtatgat tgtagatatt tatgggatct
      421 ttcgaatgat ctttgcggct tttaacgtga gttttggtgg agttgctgga tttgttcgtc
      481 caatcatttt accgatggca ttggggacca tcgaatcaaa agggttagaa gtccatccta
      541 agcatgaaga agaactaaaa ggtatggctt cagctatgga aaatgtctgc tggttctttg
      601 gccaagtact attcatcgga ggagcagggg cgttgctagt ccaatcaaca ctgaaagatc
      661 taggatatga cgtaacattg ggacagcttg ctttagtgga agttccagtg gctatcacgg
      721 ctttagttgt ggcaagtgtc tacttctatc ttaaagaaaa gaaattagcg aagaaatatt
      781 atccaggaaa ggggcaataa gatggacttt tttacaaatc ctgatatttt attaggcgat
      841 aaattactgg agatccttta tatcgtgatg ggactcgtat tgatttacac tggagtaaga
      901 aatttaacag ataaaaccaa tcctcatcgt tatgggtcag cctttttctg gacggtctta
      961 ggggtagtta ttgcaggagg aagatggctt ccggcaatcg tgaacggtgg cttgatcttt
     1021 gcgatgacca taccagcaat tgctaagaaa gtcagcaaag gagaaagccg gcttccatca
     1081 aaagaatata tggaaaaaat gtcagacaaa ctaggaatga aaatttttat tccagctttg
     1141 agtatcggtg tctttgcgat cttgtttgct ctatttacca atttaggtgc tttagtcggc
     1201 gtgggcgtcg gtgtttttgc agccatgcta atcttgatgt tcttttctcg agataatcga
     1261 ccaagtacat ttttagatga tgcagcagat atgctgggca cagtagggcc gttaagcatg
     1321 ttgccaatgc ttttagcttc tctaggtgcg gtatttacaa gcgctggagt tgggacagtg
     1381 atttccaatg cagtaggtac gatcgttcca gaaggaaatg tgaacatcgg catcatcatt
     1441 tacgctcttg gcatggtgat atttacggta atcatgggga atgcatttgc ggccatcacc
     1501 gtgatgacgg ttgggatcgg tgcaccattc gtatttagtc atggagcgaa tccagcgtta
     1561 ataggaatgg ttgcattgac ttgcggtttt tgtgggaccc tgctgacacc gatggctgct
     1621 aacttcaata tcgtgcctgt agcgatgctg gagatgaaag acaaatacgg agtgatcaaa
     1681 aaccagttgt ttattgcgtt atttatgcta gttttccaaa ttgcttatat gattctattt
     1741 aaataataga agagaggggt tgtgacagaa taattgtcgc aacctcttct aacattgtag
     1801 tggaacacta tttttttata agaatatatt aagtttgatc tgttatgtta tttaaactat
     1861 tgtttccttt atttacgctg ttcaaggaaa atggtatgat aaaagataac catatgttaa
     1921 atcaatcgga cggaggaaca acagatgaaa gtactagtaa caggttttga tccatttggt
     1981 ggggacaaag tgaatccagc ctatgaagca gtaaaaaaaa tgccagatga aatcagcggt
     2041 gctgaaatca tcaaagtgga agtaccgacc gtattcgaaa aaagcagtca agtagtcaaa
     2101 gaagccatcc aacagcacca accagatgtc gtgatctgtg tcggacaagc aggtgggcgg
     2161 tcagcagtct cgttcgaacg tgtcgcaatc aatctagcag aagcaagaat ccctgataat
     2221 gaaggaaatc agccattcga tacggcttta gaagaaaacg gtccagccgc ttactttacc
     2281 tcattgccaa tcaaagcaat gacaaaaaat gtacaagatc atggtctacc agcgtacatc
     2341 tcttatacag caggaacatt tgtctgcaat gatatcatgt atcgtttgct tcatttgatc
     2401 gaaacacaat tcccggcaat tcgaggtggg ttcatccacg tgccattctc acccgatcaa
     2461 gtgatcgatc gtccagtggg tacgccttct atgtctttag aagacattgc ttcttctttg
     2521 acttatgcag ttgaagcagc agtagaaaat aaagaagata tttccggaaa tgccggaaca
     2581 actcactaat ttagcaagaa acactggctg gttgctaacg ttatgttagt agccagcttt
     2641 ttgtaattag aaaggaaaga aacagccgtg gaagaatggc aatccgtctt tgaagaatgg
     2701 tttccaaaag aaatcagcaa aagctatccc atcaaaattt ctaaacaata cacaagcagt
     2761 caacgttggg agatctatgc aaaattaacg aaaaaacagc gggaactagt cgataagcac
     2821 cgccgatatc taatcagctc tcgttttatg gaagaacatt atttagctgc aacagattgg
     2881 gtgttttctg acttcaagat caatccgttt tttcgaacaa aacgaagcca gcaaaaattg
     2941 tattgtgagt gtggtagaga attaaaagtg caatacatcg ttaaatcgcc aaaaaccgga
     3001 aagatcctaa aactaggcat caaccacttt gccgatcacc tgcatgtctc tccgacagtg
     3061 gcagcttcga tccatcaggg aatgacaaaa gttgatctgg ccttagacga attattatgg
     3121 cttaagcaga agaatatcga ttttcctgaa ggactatggc aaaaatactg ttttgtcttg
     3181 taccaaaatc gtcggatgaa acagccatat ttaccggata taaaattagc tcagcggctc
     3241 gcagaatttc gccaagtaga aatgccgatt tacatagcgg attatcaagc attggaaaat
     3301 gaaatcaaga aaataagtga acacatcaat gggcagtcaa aaaagcgaca gataaaaaaa
     3361 gaactatttg acgactttgc agaagaatta gtaaaggatg tagaagaatt cttgatcaac
     3421 tatcgcgcat ttttacgtaa agattggcaa tccatcgtat atgaagaagt gccagtacat
     3481 cctaatgctt attttgaaac atttatttcc gtgttgagaa agacaaaacg tcaacgaaca
     3541 ccagaagtca ctgctcagat ggaatatttt gcgaaaaacc aacgattcat ccaaccaaaa
     3601 atctatctat ttatttggaa gcaatattgt cgctatggtt ttacagaagg gttcttcgac
     3661 agcattcctc ggatagtcag aaatggtttt ttaaaagtat tgcgtaaaga acgagaagct
     3721 atacaatctg ctgataagaa agaccgtaca gtctcaaaag aaaaatggca actagtagtg
     3781 aaagatatcc aatctggaaa tgtgcaagag acaatcgaca aatggaaagg aaaacattat
     3841 cgttttactg aagctcagaa acaagcgttg gaatattatc agaagttaga agaatcactg
     3901 cgtttcaatg atgaggcaag aaagtatctg aaggaattac tataaaaaac atactaagat
     3961 tagaggggaa catcattgac gaatgatgtt ctcctctatt ttctattttt agattatagt
     4021 gaagagtgaa agaaagttcg aacaaagcct tgaggactac gagtccctat caaaaaccga
     4081 acttctttac tgtcatttta gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat
     4141 aggaaattgg aatcgaaatg aagcagtaaa gttctttcaa aacttaaaaa ggaggaaaac
     4201 aaatgcctta cattcttgca ttagatgttt caatgggaca cagctacagt gtcctataca
     4261 aagacgacac ctgcttattt gaagacgaga tcgaacacac tcaaagagga tttcatgcat
     4321 tactcgaaga gatccagcac ttgcctgaac gtccattgat tgtttttgaa gcaacgggga
     4381 tttattccag accagtcgaa aagttctttc aagatcatca cttttcttac tgtttgttaa
     4441 acccgttaga agcgaaaaaa cagatggaag aaaacacctt atgcagttgg aaaacagata
     4501 aatcggatgc ccatcggctc gcacagactc atcttaaaaa tcaacgtcag ccaaaagaag
     4561 tacaaagcaa tttttactta gagatgcgcg atttagcccg tttttatcaa gaaattgaga
     4621 agaaaatcac tcgtctgcgc atggatttac acaactgcct tcagttgact tttccagagc
     4681 ttgaacagtt ctttttaaac aggctgaccc cttacgcatt gatattgatc cgtctttttc
     4741 cccatccaga ttttgtattg gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg
     4801 aaacacggaa aaaaatctcc gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact
     4861 atgcacagtg tgcctatcca gctgttgaaa aagacagtat ccactgtcaa aaaacgatct
     4921 attacgctga acttctccaa gatttactcg aacaaaaa
//
LOCUS       DACCWV010000166         4916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCWV010000166 DACCWV010000000
VERSION     DACCWV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.171"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..739)
                     /locus_tag="IVW84_002621"
     CDS             complement(<1..739)
                     /locus_tag="IVW84_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7918780.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSN"
     gene            complement(853..1437)
                     /locus_tag="IVW84_002622"
     CDS             complement(853..1437)
                     /locus_tag="IVW84_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7918781.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(1564..1995)
                     /locus_tag="IVW84_002623"
     CDS             complement(1564..1995)
                     /locus_tag="IVW84_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7918782.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(2013..2333)
                     /locus_tag="IVW84_002624"
     CDS             complement(2013..2333)
                     /locus_tag="IVW84_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918783.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            complement(2765..2944)
                     /locus_tag="IVW84_002625"
     CDS             complement(2765..2944)
                     /locus_tag="IVW84_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918784.1"
                     /translation="MNKKTDEIAADLVVAWLNHESQVSTKGESISPKEIAQAYLDIHY
                     AVIYGQLPEDRKNDD"
     gene            3025..3771
                     /locus_tag="IVW84_002626"
     CDS             3025..3771
                     /locus_tag="IVW84_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7918785.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3786..3989
                     /locus_tag="IVW84_002627"
     CDS             3786..3989
                     /locus_tag="IVW84_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7918786.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            4005..4325
                     /locus_tag="IVW84_002628"
     CDS             4005..4325
                     /locus_tag="IVW84_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7918787.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(4517..>4916)
                     /locus_tag="IVW84_002629"
     CDS             complement(4517..>4916)
                     /locus_tag="IVW84_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918788.1"
                     /translation="SSIKKIPFITLNKNSILKEFNNIDYIAFLKLFSVCVLISCVIIF
                     IVAKTCWTMLNKNQRDVENIAIFLIKITSSISIIIPMTVTFSKEQFNVAATYLSFIAL
                     FSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
BASE COUNT         1607 a          824 c          815 g         1670 t
ORIGIN      
        1 tatttgaagt ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat
       61 taatagtgat gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag
      121 caaatcgcat tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag
      181 aaaggttttc ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat
      241 cttcttttgg cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg
      301 gaattaatcc ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa
      361 taacggtaga ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc
      421 tatctaaatc agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt
      481 cgtattttct ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct
      541 caaagtaatc tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat
      601 attctaattc gttggcggca tcttttgctt cgccttttgt cttaaatcct gattttctta
      661 tcttcgtgta ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac
      721 gtttgactaa tcttgccata attgaatcta ctctctttct ctgatacaat aggcactata
      781 aagaagccta ctgtataggt ttgtttttct tagaacacgc ttgctttgga cggtgggcgt
      841 gttttttatt tattatacag ttgctttaaa aatagctgct ttttcaaatg atactaaagg
      901 agagaagtgg atttctattt ctcccttttg gtttaacgtg aaatgagcag ttacatccat
      961 ttgttttcct ggagcaacag atcccattgt attttcgttg gcatatgtct ctgatttttt
     1021 gtcatctggt ccatatactt ccacatctgt acctacaggg atatctgaat cgccatcatt
     1081 ttttacggta taagtaattt ttactacttg tgcaggttgg ttttcttcaa attgatttct
     1141 ttcatcagtt agttctacac tatttagtgt atattcagca tcaccaactt ttacagtgtc
     1201 accaatctta tagaaggtat cgcttttttc ttctttagaa gatgaagcag aggttgaaga
     1261 tttagttact ttttcgccac cattatcact tgctttatta ctatctgatc caccatttaa
     1321 agcagaacca ataattataa ttaaaattac tgctaatacc caaaaccata cttttttgta
     1381 aaaaggttta ctaactttat acatttttcc gtcttgaccc ataacttttt ttgccattta
     1441 aatattcctc acttcttgtt ataatatatt tgcgatctca gaaatgaggt atgagtccgt
     1501 gtgccagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata attcttgatg
     1561 tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt ggcagacagc
     1621 ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt tttcgagatt
     1681 aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa ttagaatagc
     1741 tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat cgtacaaaac
     1801 tgaatattca aaatgagaag cggtgaaatg cgcaagttca tgagataaat gaaaggcttc
     1861 tgcagtttcg ccatatagat tttcattcaa aaaaatgatt ctaggcttcg gataatagaa
     1921 gccaggctct ttcatttcca tgtaaactac tttcaaattg tattcgctca acatttcttt
     1981 caatttcaaa tacatacaac ccatcactcc aactattcat tttcctctaa agctttagca
     2041 attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca
     2101 gtatcgtttt ctgataaatc cacatgttta ggggtttctt tttcttcgcg ccccagaaga
     2161 tagtctacag agacatcgaa atagtcagca atttcttgca aacgttctgt agaggggttt
     2221 gagttcttaa gtctatataa gacgttttta ccatagccta ggtcttcttc aactttattt
     2281 aatgattttc cctgtttttt cgctaactct tttattctct caaacgttat catatcaata
     2341 ttcctttcgt tcaagaaaaa atatttaact ttatttgtta aaattcgttg actattttaa
     2401 caaaaggtgt taatatcatt cttgtaaaca agtttaacaa ctaaaaagac aacaaaaaac
     2461 actatcgatt aataaatgcc aaccgccaag aaagctttta aaatcaatgt ttatatgtct
     2521 tatttaacta tgttctgatt ttaacactgt atgttaattt agtcaacgca gtttttttaa
     2581 aagttgttaa atttgtttac gaatataaaa gaaaggagag aagaatatgg aaaaaaacag
     2641 tctcggcaaa aatcgaagat ttgaaattag acatactgaa acaagcaaaa gtggcgatgg
     2701 aacacgcggt agataaagaa gactctgcca tggttgcagc catagcagag attttagctc
     2761 acgtttagtc atcgttttta cggtcttctg gaagctgacc atagataaca gcataatgaa
     2821 tatctaaata agcttgagca atttcttttg gcgaaattga ttcgccttta gtagatactt
     2881 gtgattcgtg atttagccat gcaacaacta aatccgcagc gatttcatca gtttttttat
     2941 tcatacttat tcaccacctt atgcattatt tcagtagacc acttactgat aagaaaatta
     3001 tatcaaagaa aggaatgaac aaaaatgaac acaccacaaa tttttagttt tgaacaacat
     3061 gaagtaagaa cgtttctaga aaatgacatt ccgtatttcg tagcaaacga tgttgccaaa
     3121 actttgggat acaaaaatcc cagtaaagcc accaatgatc attgtaaaaa atcaattgaa
     3181 acatggggta acgattcgtt aggtcgtcgt caaaaattca aagttattcc agaatcagat
     3241 gtttaccgct tgattattaa atcgaactta ccaagcgctg aaaaatttga ggcctgggta
     3301 atggaagaag tccttccaac aatcagaaaa acaggtagct attcaaacgt acctcaaagt
     3361 tttgcacaag cattgcgttt agcagcagat ttagaagaaa agaaccaatt actcgaacaa
     3421 caaattgccg agtacgaacc aaagattagc tacttagata cgattctttc atcgacagat
     3481 acggtagcta cttctcaaat tgcagctgat tacggaatgt cggcaattgc tctaaacaaa
     3541 ttgctaaacg agttaggtgt tcaacataaa gttagcggac aatggatact ttaccgaaaa
     3601 catatgaacc aaggatacac aaaatcgcac acaagtgaga ttccgaaagc cgatggcggc
     3661 actaaagttg taatgaatac caaatggaca cagaaagggc gagtgtttat ttacaactta
     3721 ttaatcgcag agggctatta ccctcaaatg gatttagagg aaattggtta gaaaggagtt
     3781 ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc aaagaatatg
     3841 tcgaaagttc aaagttctta acttacacca tgcttgctga aagattcggt attagcaaaa
     3901 gctacttatc tttgatttta aacggtaaaa agacttctgc agaagcaaat agaattatag
     3961 attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa tatctagaag
     4021 cgaaaatccc agttccagaa ggctatgtaa ttatctctca agtggattat gaggagttaa
     4081 aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa cggattaaca
     4141 gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga aatattatcg
     4201 atatcgaaaa aaataaaaat ggatttgtct attatccagt tgaaggaaga gacacatatc
     4261 tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa atcttaagga
     4321 ggtaagcgga tgggaaaatt taacagagca ttagtattca gcgcaccact aatcatctac
     4381 gctttaggac tttggggaag cagacaagcg ttgataggaa cgattgttta catggtctgg
     4441 atttttatgg ggcttgatga agctgagtac agagcgaaaa agccagtcgg gagggactga
     4501 ctaaaatatg atttttttat ttttgttcct ttgggctgct attgcgtact tcatccagct
     4561 ccttgttgtc aaagttgctc ttatttcttt taggaagaac aaaagaaaat agcgctataa
     4621 atgaaagata agtagccgct acattaaact gttcttttga aaaagttaca gtcatcggaa
     4681 tgataattga tattgaggaa gtaatcttaa ttaaaaatat agcaatattt tcaacatcgc
     4741 gttggttttt atttaacatt gtccaacatg ttttagcaac aataaaaata attacacatg
     4801 atattagcac acagactgaa aaaagtttta gaaatgcgat gtagtcaatg ttattgaatt
     4861 ccttcaaaat actattttta ttcaatgtaa taaaaggaat ttttttaatt gaagac
//
LOCUS       DACCWV010000167         4742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCWV010000167 DACCWV010000000
VERSION     DACCWV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.172"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            23..694
                     /locus_tag="IVW84_002630"
     CDS             23..694
                     /locus_tag="IVW84_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7918789.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="IVW84_002631"
     CDS             753..1193
                     /locus_tag="IVW84_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918790.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="IVW84_002632"
     CDS             1205..1873
                     /locus_tag="IVW84_002632"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7918791.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="IVW84_002633"
     CDS             1937..2560
                     /locus_tag="IVW84_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7918792.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="IVW84_002634"
     CDS             2616..3002
                     /locus_tag="IVW84_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7918793.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="IVW84_002635"
     CDS             3215..4606
                     /locus_tag="IVW84_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7918794.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1578 a          782 c         1011 g         1371 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tg
//
LOCUS       DACCWV010000168         4721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCWV010000168 DACCWV010000000
VERSION     DACCWV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.173"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(137..1099)
                     /locus_tag="IVW84_002636"
     CDS             complement(137..1099)
                     /locus_tag="IVW84_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7918795.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="IVW84_002637"
     CDS             complement(1101..2540)
                     /locus_tag="IVW84_002637"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7918796.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="IVW84_002638"
     CDS             2725..4671
                     /locus_tag="IVW84_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7918797.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1498 a          811 c          776 g         1636 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaggc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat a
//
LOCUS       DACCWV010000169         4715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCWV010000169 DACCWV010000000
VERSION     DACCWV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.174"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            146..421
                     /gene="rpsP"
                     /locus_tag="IVW84_002639"
     CDS             146..421
                     /gene="rpsP"
                     /locus_tag="IVW84_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7918798.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            433..684
                     /locus_tag="IVW84_002640"
     CDS             433..684
                     /locus_tag="IVW84_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7918799.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            809..1600
                     /locus_tag="IVW84_002641"
     CDS             809..1600
                     /locus_tag="IVW84_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918800.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1770..2291
                     /gene="rimM"
                     /locus_tag="IVW84_002642"
     CDS             1770..2291
                     /gene="rimM"
                     /locus_tag="IVW84_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7918801.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2281..3042
                     /gene="trmD"
                     /locus_tag="IVW84_002643"
     CDS             2281..3042
                     /gene="trmD"
                     /locus_tag="IVW84_002643"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7918802.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3178..3525
                     /gene="rplS"
                     /locus_tag="IVW84_002644"
     CDS             3178..3525
                     /gene="rplS"
                     /locus_tag="IVW84_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7918803.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3783..4076
                     /locus_tag="IVW84_002645"
     CDS             3783..4076
                     /locus_tag="IVW84_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7918804.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1666 a          813 c          927 g         1309 t
ORIGIN      
        1 tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa
       61 gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat
      121 aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc
      181 taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg
      241 tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa
      301 agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa
      361 catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata
      421 aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc
      481 acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat
      541 ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa
      601 gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat
      661 atcatcgatg ataaggaaaa gtaaataaac ggtctgtact cgaattttcg agtgcagctt
      721 ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat
      781 ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc
      841 tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag
      901 cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac
      961 tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga
     1021 acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat
     1081 cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct
     1141 cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata
     1201 tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa
     1261 tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc
     1321 aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga
     1381 aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct
     1441 accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc
     1501 cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat
     1561 gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag
     1621 cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat
     1681 aattttggct taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa
     1741 aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg
     1801 taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag
     1861 aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat
     1921 taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc
     1981 cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact
     2041 ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg catgtattag
     2101 aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt
     2161 gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca
     2221 aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg
     2281 atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc
     2341 atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat
     2401 tattcagata acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg
     2461 ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat
     2521 gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca
     2581 gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat
     2641 gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg
     2701 ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta
     2761 gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa
     2821 tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat
     2881 cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg
     2941 cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc
     3001 aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct
     3061 ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta
     3121 cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg
     3181 aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc
     3241 cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag
     3301 ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta
     3361 cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt
     3421 gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt
     3481 gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac
     3541 tgagaaagaa cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct
     3601 gatccagttt tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct
     3661 ataagtagta gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta
     3721 ataaaaaatc cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga
     3781 agatggttta tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg
     3841 atggaaaagt aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag
     3901 tcttgaaacg ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc
     3961 tgaacgtatt ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct
     4021 ttttcaaaag aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt
     4081 tttaatttat actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact
     4141 tgaacttttt ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg
     4201 atagctatga taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc
     4261 gttccaggag cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc
     4321 ttttatttgt acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc
     4381 cctttaaaaa gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga
     4441 ggttgtgaaa acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact
     4501 cgtactttac cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg
     4561 atgagggaca caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat
     4621 ttaaaacaaa gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc
     4681 ttccaaattt aagcaatact tcaattaatc aagtt
//
LOCUS       DACCWV010000170         4613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCWV010000170 DACCWV010000000
VERSION     DACCWV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.175"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..1095
                     /locus_tag="IVW84_002646"
     CDS             34..1095
                     /locus_tag="IVW84_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7918805.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1098..1994
                     /gene="murQ"
                     /locus_tag="IVW84_002647"
     CDS             1098..1994
                     /gene="murQ"
                     /locus_tag="IVW84_002647"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7918806.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2008..3990
                     /locus_tag="IVW84_002648"
     CDS             2008..3990
                     /locus_tag="IVW84_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918807.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4055..4501)
                     /gene="nrdI"
                     /locus_tag="IVW84_002649"
     CDS             complement(4055..4501)
                     /gene="nrdI"
                     /locus_tag="IVW84_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7918808.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1495 a          820 c         1046 g         1252 t
ORIGIN      
        1 aaatgaagac ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac
       61 gattctttgt cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc
      121 ggtattttca cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg
      181 atcgaactag gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga
      241 gaggcattgg aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt
      301 gtaacaggtt taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat
      361 caattgaaaa tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa
      421 gaagcagaag ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa
      481 acagggctgg acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc
      541 cttgtccaag gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg
      601 ccaactttgg aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca
      661 agtaacacgg atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa
      721 tttgcttctt tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa
      781 ttttacgaat atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata
      841 cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg
      901 cgtatgaaag ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc
      961 caattaacca aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc
     1021 aaagaagagc taccattgat caaccaaatc aaagctggta tgaactatca atttataaga
     1081 aaagaaggaa gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa
     1141 accaagaaac ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca
     1201 aagaagatca gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga
     1261 ttgctaacac gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa
     1321 caagtggaag actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac
     1381 cggaaatggt cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag
     1441 gggccgaaga ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa
     1501 atgatatagt tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag
     1561 attacgcaaa atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa
     1621 tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct
     1681 ctactcgatt gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca
     1741 gtatgattgg tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg
     1801 aaaaactaat cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa
     1861 cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt
     1921 tgacaaatag tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa
     1981 aagggacctt atgaggggga aaagaccatg gcaaaaaacg aactgacttt ggaagaactg
     2041 gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg tagaaagcat tgtccactgc
     2101 atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc tgaattaaaa
     2161 gcgattccag gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat catcggacct
     2221 gggaaagtga acaaagtagc acagcaaatg gttgatttgg caggcgtgaa attaggagaa
     2281 aaactttctt ctgtaagtac agctacagca aattcatcag catctggaaa agaccgtgta
     2341 aacgaacggg cacaagaaat gaaagcggca caaaaatcga aacaaaaacc atctaaaatc
     2401 aaatcggtat taaaagacat ttctaatatc tttgtgccaa tgattcctgc atttgtcggt
     2461 actgggatcg tggcagggat tgccgcagtt ttagcaaact tagtcacagc cggaacactt
     2521 gatgcagcaa catggcaaca atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt
     2581 agctacctag ttatctatac aggtatcaat gctgcacaag tttttggtgc aacaccaact
     2641 cttggtggag ttatcggggc cgttgtgatg ctgacaggga tgaatccgga agcaccactg
     2701 aaaaacttat ttactggtga agctcttgca gctggacaag gtgggatcct aggtgtcatc
     2761 ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc ataaagtagt acctgatgcg
     2821 gtagatatca tcgttacacc aactctttca ttgattgcca tcggtctgat tgaaattttc
     2881 ttcatcatgc ctttagcagg tttcatttct gatggcatgg tcggtgggat caactgggta
     2941 cttggcgttg gtggagcatt tgccggcttc gttctaggca cattattctt gccgatggtt
     3001 atgttcggac tgcatcaaat tttgactcca atccatgtgc aaatgattga tgaaactggt
     3061 cgtacgctat tgcttccaat tcttgcgatg gctggtgcag gacaagtggg tgcagcattg
     3121 gcattatgga ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa aggagcttta
     3181 ccagtcggga tcctaggaat cggcgaacca ttgatttacg gagtaacatt acctctagga
     3241 cgcccattta ttactgcttg tatcggtgga ggtatcggcg gggcagtgat cggaatgatc
     3301 ggaaatgtcg gggctatcgc tattggacct tcaggagccg ctttgatccc attgatctct
     3361 gacggtaaat ggttaggcta catccttgga ttattggcag cttatgctgg tggcttcgtt
     3421 gctacattct tcttcggtat tcctaaagac cgtttagaaa aagaagaatt agcgactgaa
     3481 acagccacag taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg
     3541 actatcctct catcagttgc tgacggaaca gttgtttctt tagataaagc aagtgatcca
     3601 gtttttgcac aaaagatgat gggtgaaggc tactttgtag aaccagaaaa cggacatatc
     3661 tattctccag ttactggggt agtcagctct gtcttcccaa cgaaacacgc tattgggatc
     3721 acaacgccaa atggtttaga aatcttgctt catatgggaa tcaacacagt tgacttaggc
     3781 ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag tgacacctga tacgcttgta
     3841 gcagaagctg acttagaagc gatcaaagca gcaggcaaag aaacatccat gatcgtcttg
     3901 gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca
     3961 aaagcacctg tgatggatgt caaagcttaa tatgatagaa acaatagaaa cgaaagaaga
     4021 aactgaaatc tatttttcag cttcttcttt tttattattt attgttttct gctaaaatct
     4081 gataaattcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc aagaaaggaa
     4141 agccaaatgt ttgtgcatat tgtttggctg ttagacaata ctgattattg aagtttttat
     4201 ttccgctacc gactacacca agacaaagtt tatgattgtc ttcatgttcc agatattcac
     4261 gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa accatttccg ccgtctaaat
     4321 aagtaggaac gaaagtaaaa aatggttctg tttctttcgt aaaatcacta ttttcatgga
     4381 tttcttttaa agtaacttca ggattgacag gatcttctgc atgcattttt tcagcatatt
     4441 ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat cgatatatat aaaatgttca
     4501 tggatcatcc tccaattctt tccttttagt atagcatctt ttttaagttt tttggtggtg
     4561 taatttggaa agggactagg cgctttgctt gtataatgag aaaaaagccg gta
//
LOCUS       DACCWV010000171         4599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCWV010000171 DACCWV010000000
VERSION     DACCWV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.176"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..734
                     /locus_tag="IVW84_002650"
     CDS             <1..734
                     /locus_tag="IVW84_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7918809.1"
                     /translation="NKIDFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDM
                     YGLPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFG
                     IPVGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIR
                     NTEIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKF
                     LYQYIGKKVKRPISADSVLLEEDVE"
     gene            731..1879
                     /gene="neuC"
                     /locus_tag="IVW84_002651"
     CDS             731..1879
                     /gene="neuC"
                     /locus_tag="IVW84_002651"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7918810.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1852..2529
                     /locus_tag="IVW84_002652"
     CDS             1852..2529
                     /locus_tag="IVW84_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7918811.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2519..3661
                     /locus_tag="IVW84_002653"
     CDS             2519..3661
                     /locus_tag="IVW84_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7918812.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3674..>4599
                     /locus_tag="IVW84_002654"
     CDS             3674..>4599
                     /locus_tag="IVW84_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7918813.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYT"
BASE COUNT         1673 a          591 c          900 g         1435 t
ORIGIN      
        1 aaaataaaat tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac
       61 aaattggtgt acaaggattt aaaattggct caggagattt aacaaattta ccttttctta
      121 gaaaaataga tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg
      181 aggtcagtga ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt
      241 caaattatcc ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa
      301 tgttcgggat acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg
      361 ctgttgcgat gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg
      421 gtccagatca taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta
      481 gaaacactga aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta
      541 caaaagaagt atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc
      601 ttacagagaa atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat
      661 atcaatatat tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg
      721 aagatgttga atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata
      781 taaacctgtt gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg
      841 aatgcattta tcccataaat atggatttac taaagaattg atcttagctg acggatttag
      901 aattagtgga gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc
      961 tgtggcatta gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt
     1021 attattacta ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg
     1081 aatcaaaaca gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag
     1141 acatgccata acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg
     1201 tttaataaaa atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga
     1261 tactatttta aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa
     1321 atttgaaaag aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa
     1381 tatagaaaaa caaattttag ctttgatcaa ttcaatggaa caacaatctc aaccaattat
     1441 ttgcatttta cctaatagtg atgctggtag cgaaattatc agaaaaatgt accagaaata
     1501 tttaaaaacg gatattattt atattacgaa tttagagccg gaggaatacc tgactatatt
     1561 aaaagagtgc aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt
     1621 caaaatacca gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat
     1681 tataaatgca aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc
     1741 tcagtttata aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag
     1801 aagaataaca aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca
     1861 aaaacaaata gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga
     1921 cacgcaaaag tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt
     1981 gatgataagc aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat
     2041 agctgggcgg atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta
     2101 gtaagaaaaa agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt
     2161 catccaagtg ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg
     2221 aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc
     2281 attgttgagc atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta
     2341 gcgggtggag tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag
     2401 atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt
     2461 ccctcacatt ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt
     2521 gaattctaga attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc
     2581 taaggctttt aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga
     2641 aagtattaca aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc
     2701 tgccatacat cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc
     2761 ttcatttact tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt
     2821 cattgactca gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga
     2881 agaagctgaa agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca
     2941 aatggctgat tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga
     3001 agatgcagcg gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac
     3061 aattgggatc tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct
     3121 agtgtctagt gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga
     3181 tcaagcaccg tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc
     3241 tgccggtatc ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga
     3301 aattttcaat cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga
     3361 agtggatggc atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc
     3421 tattgatcaa gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc
     3481 tttgtggaaa ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga
     3541 taataatctt agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag
     3601 taatatgaca gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta
     3661 ggtgtgatat tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact
     3721 tagactcatt caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc
     3781 gagacgaaag gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc
     3841 aaaaaatttg tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt
     3901 tattataaaa gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa
     3961 tgaatattat gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc
     4021 ttctacattt ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa
     4081 aaaaatttct tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga
     4141 ttatcgcaat ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat
     4201 tgaattacct tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca
     4261 gaaattatcc aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga
     4321 aactggtata gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt
     4381 aactataatt ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc
     4441 atggaatgaa gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga
     4501 taataattat tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt
     4561 cacgcttttg gaaaagaaaa tggaacccca atatacttt
//
LOCUS       DACCWV010000172         4596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCWV010000172 DACCWV010000000
VERSION     DACCWV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.177"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..1259)
                     /locus_tag="IVW84_002655"
     CDS             complement(27..1259)
                     /locus_tag="IVW84_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7918814.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="IVW84_002656"
     CDS             1514..2083
                     /locus_tag="IVW84_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7918815.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="IVW84_002657"
     CDS             complement(2261..2701)
                     /locus_tag="IVW84_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7918816.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="IVW84_002658"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="IVW84_002658"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7918817.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="IVW84_002659"
     CDS             3655..4578
                     /locus_tag="IVW84_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7918818.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1387 a          856 c          911 g         1442 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattg
//
LOCUS       DACCWV010000173         4595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCWV010000173 DACCWV010000000
VERSION     DACCWV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.178"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            189..2858
                     /locus_tag="IVW84_002660"
     CDS             189..2858
                     /locus_tag="IVW84_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7918819.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3569)
                     /locus_tag="IVW84_002661"
     CDS             complement(2949..3569)
                     /locus_tag="IVW84_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7918820.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="IVW84_002662"
     CDS             3683..4222
                     /locus_tag="IVW84_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918821.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="IVW84_002663"
     CDS             4219..4557
                     /locus_tag="IVW84_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP7918822.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1424 a          790 c          860 g         1521 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac attta
//
LOCUS       DACCWV010000174         4536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCWV010000174 DACCWV010000000
VERSION     DACCWV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.179"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1073)
                     /gene="lacD"
                     /locus_tag="IVW84_002664"
     CDS             complement(99..1073)
                     /gene="lacD"
                     /locus_tag="IVW84_002664"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7918823.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(1070..1528)
                     /locus_tag="IVW84_002665"
     CDS             complement(1070..1528)
                     /locus_tag="IVW84_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7918824.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1525..2988)
                     /locus_tag="IVW84_002666"
     CDS             complement(1525..2988)
                     /locus_tag="IVW84_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918825.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2970..3902)
                     /gene="pfkB"
                     /locus_tag="IVW84_002667"
     CDS             complement(2970..3902)
                     /gene="pfkB"
                     /locus_tag="IVW84_002667"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7918826.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4044..>4536
                     /locus_tag="IVW84_002668"
     CDS             4044..>4536
                     /locus_tag="IVW84_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7918827.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDS"
BASE COUNT         1351 a         1025 c          722 g         1438 t
ORIGIN      
        1 acttttgcag ttttatactt tttttctata aaatttgaca aacatttttt taaataacta
       61 attatttttt gaatctgtca ctcagacaat cctttctctt acttgttaac ataaatagaa
      121 gttgctgttt cttctaatag tgtacttaat tctttaatat tttgttttcc ttcttctaac
      181 atccaactac tcgctttttc ttcgccttcg gtaacaaaag gtgctactcc cttcgcccat
      241 gttgctcgcc cacataagac accattgaat gtcgaacctg attgttttgc aaaacgcaat
      301 gtatcttgaa atagtgcagc gctgactccc gcacttaaaa aaataaacgg ttgttcagtc
      361 actttcgatt gttcatagaa atacgcggat gcctctgctt gactataaca agtatcttgc
      421 ccatatcctt ccacaaatct catattcact ggtacttcta ctttcaatac atccacttga
      481 taacgttctt ttgaaaattc tcgcatggct tcaatcactt tatgcggttt tacttttgca
      541 tagtcaagag aagacggatc gccaatagtt gcatcgtaag aaaggagctc taaaaagaaa
      601 ggtatgcctt ctgcttcgca ttctgaacca atccgttcta taactatttg tttttgcaca
      661 ttaatctcta ctggttcatc aacgtcatag tacaataaaa atttacaagc atccgcaccc
      721 acttctttta accttttcac tgaccaacta ttcagtaaat ctggtagtcg gcctggcatt
      781 ttcgcatcat agccggtttt ttcataagct agcaacaaac cacattcttt ctctcgaagc
      841 gctgctgcag gcagaccata ttctggatcg agtaaaatag cggaagcata cggagtcagg
      901 tactgagaaa ccatctgttt aaattggatg atatcttctg atgtagcttc tttatattgg
      961 ttgatcattt tcttcaaagc accacgttgg tcgatagcta gcgcgctaat aattccagct
     1021 tcatccgtca atcgcttcaa acgttcctgt ttttctatac tcattttctt catatatctt
     1081 cacctatttc tttagttaat attgtagcta tttctatttt tgattttgca gctctcaact
     1141 gttctgtaac ctcttttcgc atcaatgatc gtgcaacgag agacaataat ttcaaatgag
     1201 tcgttcctgc ttctgaatct gggataaata acgcaaaaat aaaatcaact gactgtctat
     1261 ctaaactgtt ccattcaatt ccttttttca gtgtgacgat aatcaaacta ggttcattga
     1321 ttgtttgaga cttcgcatga gggatagcaa aacctgacat tattcctgta gtaccttctt
     1381 tttctctttc cagaagtttt tgatatactt ctttttcgtc atgagcgatt cctaaacaga
     1441 atgtttgctt tgctaaaaac ttaaatgctt cttcttgatt atctaatggc tgttgtaaaa
     1501 aaatgttctc ttcatgaatg attgtcatga taaacctcct gctcttcctt cttcgataac
     1561 tgtacgattt gttgctttat tatccctctt cttgtcttta tcagttactc ttttcttttg
     1621 gatcaaacct agtatcactc caccaacgat cgaaccaata agaatcgctg taacccattg
     1681 gatttttccc gtcactacag gtaacactaa aaaaccgcca tgtggggctg gtacttggac
     1741 accaaacaga taggtcaaga cagcagcaat tgatgagcct aacatcatcg ttggtaatac
     1801 tcggatcggg tctttcgcag caaacggaat agctccttcc gtaatatgag ttgatcctaa
     1861 aataaaatta accaaacctg cattacgatc attagtatca aaatatttac caaacaataa
     1921 agtagcaaaa ccagtaatca acggtggtgc aatgcaagct gctgacgcac cagccataaa
     1981 agtcgtgttt ccttgtgtca aaagagctgt tcctgttaca tatgccgcct tattaaccgg
     2041 tcctcccata tcaaacgcac acatacagcc aacaatcacg ccaagaacaa gtggattcga
     2101 atattcaaat ccagctaaaa agctcatcat tccttcattt atagccttca ttggacctgc
     2161 cagcaaagac atcacgatgc caataaatgc tacaccaata actggatata agacgattgc
     2221 cttcaagcca tctagttgtt taggtaaaat tatcagtagc ttttgcaaaa ataagataaa
     2281 ataacctgct aagaacccag ctaaaatacc tcctaaaaat ccagtaccgc cattcatggc
     2341 tacgattcca ccaacaaatc caacgactaa ccctggacgt ttcgcgatcc cttctgcaat
     2401 ataagctgct aaaactggaa ccatcattcc cattgctgca tcgtcaactg cttttaccat
     2461 tgccgctgta gcattgtatt gtgcatgatc tggatcagaa gaatagattc cccaaacggc
     2521 aaatgaaata gcaatcaata ctccgccagc taccacgaaa ggcaacatat gagagacacc
     2581 gttcatcaaa tttttataga tgctacgacc aaaattagca ccacttatcg cttctggttc
     2641 taaagtatca gcagcttcat tatctgctaa gacagtacct tttcctgcca atgcttcgtc
     2701 aatcagccgt tgtgcatcct tgattcctgt actgacagaa acatcaatga ctcgttttcc
     2761 cacaaatctt tctgaatgga catctttgtc tgcggcaata atgacagcat ctgcctgttt
     2821 gatttcttca gccgttaatt cattctcgat cccaatttgt ccatgggtct ccactttgat
     2881 cgtgatgcct tttttcctag ctgcttgctc taatgcctct tgcgccatgt aggtgtgcgc
     2941 aattcctgtc ggacaaccgg ttgctgcaat tagttgatat tttcccatct ttcgaacctc
     3001 tcttccttga ttttgatttg tttacttaat tcctccacat ccaaaaaatc agtcagtcct
     3061 ttacgaaaag cagtagagct gcctgctgct atgcttttac gtaacgtttc atcaagcgat
     3121 cgtcgattca tgtatcctgc taaaaaagtg cctaacatgg catcacctgc acatgctgta
     3181 ttgacaactt ttccttttgg cgagtttcct cgaatcaccc tgtctttcgt aaaaagaatc
     3241 gctcctttct caccgaggga taacaaaaca ttttctgcgc ctgactctac tagtcgttgt
     3301 ccataaacaa agtaatcttc atctgtaacc atttttcgtc cgaaccaaga agaaagttct
     3361 tcctcatttg gtttcagcaa aaacggtcga tagggtaaac aatccatgac ttcttgatca
     3421 ctactatcaa taatcaaaca aatccgactg atttctatta aaacctttgg agacagccct
     3481 cttggtaaac tcccagaaac acaaagataa tcccctgctt gtaatgctct tatttgattt
     3541 aaaaaattat gaacagctgt ctgcggtatt tcaggtcctt gattcactaa tttgtattct
     3601 tctcccgttt gattgacttg tgtaaataca ttgatacgcg tcataccagg cacttcaata
     3661 aactctgttt ggatttgttt ctgctgtaaa tagtcttcta tatactttcc tgtgaaccca
     3721 gcagaaaaac ctaatgccgt acttgggaaa tctagcatct ttaatactag tgacacattc
     3781 accccttttc cgtttgcctg aatgtcatcc tcttttgtgc ggtttaccat aaaaggatgc
     3841 atctcatctg tttcaataaa tagatcaatt gccaaattca tcgtgcatgt gtagatagac
     3901 ataaccaacg acctccttga ttctattatg aaatacgcga aatagaaaat gttttcttaa
     3961 tcgtcccaac acaaagaaaa cctttacatt tataaactcg tcgtgctttt tttaactaac
     4021 ttttggtgaa agaaggaaag aatatgaatc acaaattaag tgaaacagag cagtacctct
     4081 ggaactttat cgaacacctc atcctagaga tccctaacta ttccatcgtg aaactaagtg
     4141 aacaagcaaa tgtctcaaca gccaccattg tgcggacaat gaaaaagaaa ggctatgaag
     4201 ggtttacttc gtttaagcat catctaaaag aaggagaaaa caagaacatc aatttttcat
     4261 ttttagataa agtagacaaa ggaatccgcc gagccattct aaaaaatgaa caagaagtgg
     4321 tacgtacaat caatatgttg gaaatcggga acattgaaga tgcaatacaa agaatcaaat
     4381 ttgctgaccg ggtttttgtc tttgcccgag gattttcaga aatgattggc caagaaatgc
     4441 tggtaaaact tcaattaacg ggaaaaaatt gtcagatgca tactgatcca gaaattatca
     4501 aaagcatcag tcggaaacta actaagaaag acagcg
//
LOCUS       DACCWV010000175         4417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCWV010000175 DACCWV010000000
VERSION     DACCWV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.180"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            682..1650
                     /locus_tag="IVW84_002669"
     CDS             682..1650
                     /locus_tag="IVW84_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7918828.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1661..2476
                     /locus_tag="IVW84_002670"
     CDS             1661..2476
                     /locus_tag="IVW84_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP7918829.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2544..3254
                     /locus_tag="IVW84_002671"
     CDS             2544..3254
                     /locus_tag="IVW84_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7918830.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3290..>4417
                     /locus_tag="IVW84_002672"
     CDS             3290..>4417
                     /locus_tag="IVW84_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7918831.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1485 a          660 c          921 g         1351 t
ORIGIN      
        1 aattcaccaa aaaggtgagt aatgccattt ttgaaaattg agcttgtttt atgtctacat
       61 ctgtatccgt atataatcca aaggattata aactcagaag agtctttatt ttttctgaat
      121 ataactactg tatcaaaaac aatcgattat gagacaaaac aaaatgacct cttttattta
      181 agaaaggagg tcattttttg tatcataaat tgtattaaaa tcaatgtatc actttttaat
      241 cagttttaag accataaatt gcgtactaaa agaaattctg caattcaaaa taaagcattt
      301 taagccattt tcaaatttaa gtgcacccat ctacccgtca aatcacttta ttttccactc
      361 cagtagtttc ctaattcaac tgtgtgcaaa aacgatttct tctaaacaaa tcaagacaaa
      421 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
      481 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
      541 attgacaaac acaaagaaag cggatacaat gcaattatgt cacatgacgt tatatgacgt
      601 tatgtgatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
      661 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
      721 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
      781 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
      841 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
      901 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
      961 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     1021 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     1081 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     1141 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     1201 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     1261 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     1321 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     1381 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     1441 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     1501 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     1561 agtattcttt cagaatatcg ttcagcttta gcagatgtac gcaatcatga tttagatgtt
     1621 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     1681 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     1741 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     1801 ctcagatgag gaggcagctc atgttttatc agcaataaaa gaaataggac tggacaacag
     1861 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     1921 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     1981 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     2041 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     2101 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     2161 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     2221 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     2281 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     2341 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     2401 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     2461 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     2521 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     2581 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     2641 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     2701 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     2761 tcaaccggca taaagttttt cgtaaaattg aatatattat gggtttcagt gagagttgta
     2821 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     2881 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     2941 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     3001 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     3061 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     3121 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     3181 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     3241 gtttttactt atgatttcct gtttggattt caaggagtag attttattta tgaagcaaat
     3301 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggtt atattgtaat
     3361 caatgaaggg atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga
     3421 aaacacggaa ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca
     3481 tgtatttttc agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc
     3541 agctaacgag gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc
     3601 aatttatgct catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt
     3661 gaatgaaata aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg
     3721 gatgaatgct attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag
     3781 tcgagtatta ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc
     3841 tgaatttgaa gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga
     3901 tgacgcagaa tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca
     3961 ggcagtccaa gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc
     4021 tgaaaaggtc cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag
     4081 tggagatatt tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc
     4141 aattaaagca gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga
     4201 aggagataaa gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc
     4261 tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa
     4321 aatgaacgaa ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac
     4381 gttagaagag gcttacatgt caactgttat aaaaaat
//
LOCUS       DACCWV010000176         4396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCWV010000176 DACCWV010000000
VERSION     DACCWV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.181"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..93
                     /locus_tag="IVW84_002673"
     CDS             <1..93
                     /locus_tag="IVW84_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7918832.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="IVW84_002674"
     CDS             complement(151..1371)
                     /locus_tag="IVW84_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7918833.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..1996)
                     /locus_tag="IVW84_002675"
     CDS             complement(1391..1996)
                     /locus_tag="IVW84_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7918834.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="IVW84_002676"
     CDS             complement(2045..2977)
                     /locus_tag="IVW84_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7918835.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="IVW84_002677"
     CDS             complement(3398..4258)
                     /locus_tag="IVW84_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918836.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DACCWV010000177         4346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCWV010000177 DACCWV010000000
VERSION     DACCWV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.182"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(50..643)
                     /gene="clpP"
                     /locus_tag="IVW84_002678"
     CDS             complement(50..643)
                     /gene="clpP"
                     /locus_tag="IVW84_002678"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7918837.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            823..1350
                     /locus_tag="IVW84_002679"
     CDS             823..1350
                     /locus_tag="IVW84_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7918838.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            1441..2178
                     /locus_tag="IVW84_002680"
     CDS             1441..2178
                     /locus_tag="IVW84_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7918839.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2182..3099
                     /gene="miaA"
                     /locus_tag="IVW84_002681"
     CDS             2182..3099
                     /gene="miaA"
                     /locus_tag="IVW84_002681"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7918840.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3101..4330
                     /gene="hflX"
                     /locus_tag="IVW84_002682"
     CDS             3101..4330
                     /gene="hflX"
                     /locus_tag="IVW84_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7918841.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1473 a          756 c          963 g         1154 t
ORIGIN      
        1 tttatttggt ttagataaga gagcttaaaa aagcttcctt tgaaacagat taatttaatg
       61 aagaactgtt ttccatgact tcatcgatca atccatatgc ttttgcttgt tcagctgtca
      121 tatagttgtc acgatctgta tctttttcaa tcacttcaag cggttgtcca gtgcgttcag
      181 ctaaaatttt gtttaagcgt tcgcgtgttt gcaaaatatg gcgtgccgca atttcgattt
      241 cagttgcttg cccttgagcg ccgccaagag gttgatggat catgatctca gcatttggta
      301 aagcaaatcg tttacctttt gtaccagctg tcagcaagaa gcttcccatt gaagcagcca
      361 tacccataac aattgtttgg acatctgctt tgacaaagtt cattgtgtcg taaatagcca
      421 tacctgctgt aactgaacca ccaggagaat tgatataaag atagatatct ttttcagaat
      481 cttgagcgtc taagaaaagc aattgggcga tgatcgaatt agcaagatca tctgttactt
      541 ggccgcttaa catgatgata cggtctttca ataaacgtga gtagatatcg taagctcttt
      601 cacctcgtga agattgttca atgactgtag gaattaaatt cataatagag ttcctccttg
      661 atttcgttta gaaatagtgt accataattt agtttctaat acctaaaatg tagtatacat
      721 ttttggtcaa aaaaggtcaa ataaaaaact taggcaaatt agtcgcataa acccgcttta
      781 tggtatgata agcagagaaa caaaacaagg aggaacaaca gattgatagt tggtattact
      841 ggccttatcg gagtgctgac aggtgctgta gttgtctata tcggcatgat actgcaaatg
      901 cgtcacttgg aaaataaaga acagcgtaga cgtgaattgg aattgaaagt caaagaaatc
      961 gagacgctga atcaattaaa taaaaagatc aacgagatct tgcaaaaacg gacaacgctg
     1021 atggaagaat atgtcagctt tgatgcattt gatgattgtt atatcacgat tgacgatttt
     1081 gcgtatctcc aatcattttc agcgcaaaat agtttttatc tgccaaatta tattttagac
     1141 gagctattta agaatatctc ccatcgaaaa gtgattttat ctccagatga aacaatgaag
     1201 attggtggat atgcttataa aggcggacgc gtgattttag agaattttac ggatagtctg
     1261 actgaaatga tcaatgaaaa aaaagctcag ttgaagacta tgacgcgcca gccgattggt
     1321 ttcttctcga aagatgatgt ttggagctga tattttgaag cattgccgtt gcacatgaga
     1381 gcgtggtaat gctttctttt tttcttaatg atacaataga actgaacgga gtgaagaaag
     1441 atgttacaaa tctatgccca tcgcggaagc agcggcaccc acccagaaaa tacgcttcct
     1501 gcatttgcag aagctgtacg agtaggtgca gatggtatcg aactagatgt ccatctaagc
     1561 aaagatggtt atttgatcgt tatgcacgac gaagaagtgg accggacgac taacggtaaa
     1621 gggctgattc gagaaaaaac attggaagaa ctcaaaaaat tgaatgcagg gggcaaattc
     1681 agcaaagaat ttccagcagc taaagttcct gaattgagag aagtgttagg actgctcctc
     1741 caaaaaaatt accgagggat gctaatgatt gaaattaaaa ctgatcagta tgaatatccc
     1801 ggtatcgaag aaaaattagc cgaaacatta aaaagtagag attggccttt tcaacatgca
     1861 tattgcagct ttcatttgga ttctttggaa aaaatcgcat tactggaacc ggagatacag
     1921 ctggacttat tgatgagtac ttctgataaa aagatccaac tagcagcaga aaccccttat
     1981 atagaaggag tacatccaaa aaatacttgg ttgttcgaac atccagtcca ggcagctgat
     2041 tttccaaaag caattcgtgc atggacgatt aacgatgaag aagaaatggc caaatgtcaa
     2101 gaactcaaaa tagatggaat aataactgat tttccggaga aagctcttcg gtggaaacaa
     2161 tcaacgaaaa gtaggtaaaa tatggaaaaa gtattagtga tcgtcggtcc gacagcagtc
     2221 ggtaaaacag cgctaagtgt ggaactcgca caactatttg gaggagagat catcagtggt
     2281 gattcattgc aaatctataa aaatctggat atcggtacag caaaaatcaa agaagaagag
     2341 atgaataatg ttccgcatca tttgattgat gtgatcgaac ctactcaacg ttactcagcc
     2401 gctgattttc agcattcagg tcgacaattg attaccgaga ttgcgaaccg agggcaccta
     2461 cctatcatag ctggtggtac gggtctctat atccaatccc ttctatatga ttatcagtta
     2521 ggagcgacag aagaagcgaa tcctgagatt agaaaaaagt atgaaacgtt agctgaaaca
     2581 attggttccc aagcattatg gaatcagttg aaagaaaaag acccggcagc tgctgagaaa
     2641 atccactgga acaatcaacg caaagtgata agagctctgg aagttttaga gacgacagga
     2701 catagtatca cggcacctaa agaagagcca aagcaattgt atgattacct catgattgga
     2761 ttagatacga agcgtagtat tctttatgaa cgaatcaatc aaagagtcga tttgatgcta
     2821 gcagaaggtt tagtagcaga agcgcgtaag gtgtatgagc ttggagaagt ccaggccagt
     2881 caaggtattg gatataaaga atttttccct tattttgaag ggaaagaaac attggaagaa
     2941 gcaacagaac aagtcaaact acattcgaga agatacgcga aacgtcagtt gacgtggttt
     3001 cgtaaccgtt tggatgcaca gtggtttgat cttataagtg agccaaatca aaaaagtcag
     3061 attgagcaaa caatcaaaaa atggctggag gcggaataaa atggaagaaa aagtgatcat
     3121 cgtaggggta gaaaccgaag caaaccagcg ttacttttca gagtcaatgg aagagctagt
     3181 tcagctgacg aataccgcat caggagaagt cgtattcaca attacacaaa agagaccaca
     3241 agtggatcgg caaacgatta tcggtaaagg gaaattacaa gaattagttc aacaagcgga
     3301 tgcgcatgaa gcggatttga tcatctttaa tcatgagcta actccgagac agagccaact
     3361 gattaccgat gctgttggtc ttccggtaat tgatcgcgtg cagcttatct tagatatttt
     3421 tgcgatgcgt gctcgttcga aagaagggaa gctgcaggtt gaacttgctc agttagaata
     3481 cctgctgcct cgtttagtag ggcaagggaa aacattatct cgtcttggcg gtgggatcgg
     3541 aacaagaggg ccaggtgaaa caaagttaga aacagaccgc cgacatatac ggaataaaat
     3601 ttttgctgtt aagaaagaat tgaaagaagt agaagctcat cgagagcgca atcgccaaaa
     3661 aagaaaaaat agcgagattt ttcagattgg acttattggc tacacgaatg ccgggaagtc
     3721 aactatcttg aatctattga cacaagcgga tacttattcg aaagatcaat tgtttgcaac
     3781 gttagatcca ttgactaaga gatggcgttt tgctgaagga tttgagatta ctgtaacgga
     3841 taccgtcggg ttcatccaag acctgcctac gcaactgatc gatgctttcc attctacatt
     3901 agaagaaagt caaaatatgg atcttttgct acatgtagtc gatgctagtt ctccagatcg
     3961 tattttacag gagcagactg tcttaaaatt gatggatgaa ctgaatatga aagaaatgcc
     4021 gatactgact gtttacaata aggcagacca gatcgaccca gcgatgttca cgcctacgct
     4081 attcccaaat gtattgattt ctactcagac gaaagaaggt aagatggcgt tgatcgaagc
     4141 aatcaaacgt cagctgatgg aactaatgac cccttataca ctttctgtgc caagcaacga
     4201 aggacagaaa ttaagcgagt tgagaagaca aacaatggtt ttggacgaag cgtacatgga
     4261 agaaacaaac gaatatgaag ttaaagggtt tgcaaaaacc aattcaaaat ggttaagaaa
     4321 aaaccaataa aattttattt ttttaa
//
LOCUS       DACCWV010000178         4109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCWV010000178 DACCWV010000000
VERSION     DACCWV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.183"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            56..1357
                     /locus_tag="IVW84_002683"
     CDS             56..1357
                     /locus_tag="IVW84_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7918842.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1384..1644
                     /locus_tag="IVW84_002684"
     CDS             1384..1644
                     /locus_tag="IVW84_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7918843.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2643..3878)
                     /locus_tag="IVW84_002685"
     CDS             complement(2643..3878)
                     /locus_tag="IVW84_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7918844.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1167 a          666 c          862 g         1414 t
ORIGIN      
        1 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
       61 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      121 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      181 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      241 ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc
      301 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      361 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      421 agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      481 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      541 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      601 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      661 aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      721 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      781 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      841 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      901 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
      961 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1021 cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt
     1081 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1141 agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg
     1201 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1261 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1321 acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa
     1381 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1441 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1501 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1561 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1621 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct
     1681 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1741 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1801 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1861 tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta
     1921 gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc
     1981 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt
     2041 caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac
     2101 gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat
     2161 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2221 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2281 tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac
     2341 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2401 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2461 tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2521 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2581 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2641 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2701 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2761 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2821 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2881 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     2941 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3001 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3061 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3121 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3181 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3241 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3301 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3361 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3421 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3481 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3541 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3601 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3661 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3721 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3781 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3841 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3901 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     3961 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4021 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt
     4081 tgtgccagat aaatacgaga agttgtgtc
//
LOCUS       DACCWV010000179         4066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCWV010000179 DACCWV010000000
VERSION     DACCWV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.184"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            85..1434
                     /locus_tag="IVW84_002686"
     CDS             85..1434
                     /locus_tag="IVW84_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7918845.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="IVW84_002687"
     CDS             complement(1562..2152)
                     /locus_tag="IVW84_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7918846.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="IVW84_002688"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="IVW84_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7918847.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACCWV010000180         4061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCWV010000180 DACCWV010000000
VERSION     DACCWV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.185"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            27..1451
                     /locus_tag="IVW84_002689"
     CDS             27..1451
                     /locus_tag="IVW84_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7918848.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1632..2405)
                     /locus_tag="IVW84_002690"
     CDS             complement(1632..2405)
                     /locus_tag="IVW84_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7918849.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2402..3307)
                     /locus_tag="IVW84_002691"
     CDS             complement(2402..3307)
                     /locus_tag="IVW84_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7918850.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3461..3781)
                     /locus_tag="IVW84_002692"
     CDS             complement(3461..3781)
                     /locus_tag="IVW84_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7918851.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1220 a          752 c          815 g         1274 t
ORIGIN      
        1 gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt
       61 taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta
      121 tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa
      181 cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa
      241 aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg
      301 gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga
      361 gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga
      421 tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta
      481 gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag
      541 tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat
      601 tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga
      661 tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac
      721 aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct
      781 tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc
      841 agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca
      901 acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg
      961 aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca
     1021 aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac
     1081 tttcattatt attcactgac agtgcttata actttgctta cacacttggt acagcagcta
     1141 ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag
     1201 aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag
     1261 tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt
     1321 acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt
     1381 tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc
     1441 ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa
     1501 aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa
     1561 cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt
     1621 ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa
     1681 acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc
     1741 atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg
     1801 atccactccc atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa
     1861 taaaagacga ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc
     1921 aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag
     1981 ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga
     2041 atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata
     2101 actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga
     2161 tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg
     2221 ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg
     2281 atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca
     2341 gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat
     2401 gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat
     2461 cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca
     2521 cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag
     2581 tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga
     2641 tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt
     2701 gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga
     2761 tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg
     2821 cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta
     2881 aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt
     2941 gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg
     3001 tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt
     3061 cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac
     3121 caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg
     3181 tttttccagc gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac
     3241 tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac
     3301 ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg atgagtggag
     3361 aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata
     3421 aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct
     3481 tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct
     3541 tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga
     3601 taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag
     3661 caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt
     3721 tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca
     3781 tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa
     3841 agacctaatg gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc
     3901 tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca
     3961 agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa
     4021 gaagttgtta tttattaatc atttaaaaaa aacagtaact a
//
LOCUS       DACCWV010000181         4044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCWV010000181 DACCWV010000000
VERSION     DACCWV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.186"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(158..1663)
                     /locus_tag="IVW84_002693"
     CDS             complement(158..1663)
                     /locus_tag="IVW84_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7918852.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1660..2346)
                     /locus_tag="IVW84_002694"
     CDS             complement(1660..2346)
                     /locus_tag="IVW84_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7918853.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2539..3960)
                     /gene="gndA"
                     /locus_tag="IVW84_002695"
     CDS             complement(2539..3960)
                     /gene="gndA"
                     /locus_tag="IVW84_002695"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7918854.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1114 a          857 c          692 g         1381 t
ORIGIN      
        1 tcgttcatct aaaagatact tacatttaat taacggatgt tattctattt aattgttcgc
       61 tagttttttt ataatgacat ccctgaattt ctcacttgct tgtattttgt accttattct
      121 ttgttggata tataaaaaaa caagtaaagc acatgcttta cttgtcttct gcctttgcga
      181 taggaataaa gattctaaaa attgtccctt gccctactac actttctgct aagatacgtc
      241 ctttatagtt ttcaaccagt tgtttagcga tagacaaccc cagaccattt ccacccttcg
      301 ttctagctct tgctttatcc actcgataaa aacgattgaa gatcttatta aggtcttctt
      361 tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa
      421 tagagatatg gacttctttt cgtgtagttg aatactttac tgcgttatcc aatatgataa
      481 tgattaattg ttcaaagtga ttacgataaa tttgcagggt cacttctttt gtcaaatcat
      541 catctaaagt gatcacatag tctggatata atatcttgaa attattataa acttgatagg
      601 tgacttcttt tcctttagtt gtttcattcg cataatgaac atctacttgt tctgctcttg
      661 ataagtctag catctcttga actaagcttt tcatacggct gatttcttgc atgctggcgc
      721 ttaatgattc ttctaatatc tcaggatcat ctttccccca acgatttagc agactaagat
      781 gcccttcaat aattgcaaca ggtgtcctta actcatgaga tacgtcttcc acgaattgct
      841 cttgttgttc aatatacaaa cgcatacggt caagcatctc attgaatatt tcagctaaat
      901 ctgccaattc atcatttgtg tcgatttcag gcatgtggat atctgattgc ggatctttac
      961 gtatagtatc cattgtatct ctgagtacct tgagtggttt taagaaataa gaagataaaa
     1021 agaaacctaa aatactactt aagatcaaag aaacaacttc taaaacaata agagtcaata
     1081 atagcttatt tcgaatatca tagaaagaag ataattcata gaagctttgc gcatagccga
     1141 tcttttctct tgtaccttta gaatatatag gctggataga taaaaagcca gtttttcctt
     1201 ctagtgtaac gatcgttgga actttacgtg tagtctgaat caggtttcga ctgttttcat
     1261 gggttttaaa aatcaagttc tcgtccaaat cataaacata aagatccatt gatggctgcc
     1321 ctagctcagc tataaaggaa tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat
     1381 ctacgacctc atctccgtca tttgtcgctt tcgtcagatt acgatacgta tttactaatg
     1441 tcagttcttc atccgagtta gccaaacgag aagctacttc tgaaatcgta cgctctacat
     1501 tgtttctttc ttttgccaca atcagattga tagatgattt ataggtaatg acagcgaaaa
     1561 ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg
     1621 gccctttcag ttctcttttt tccgattttt tcggctttat cacgagcgca tcacataacc
     1681 tgttccgcgt acagtttgga tatagctttc ctccccggga acgtcaattt tgttgcgaag
     1741 ataacgaata tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc cccacacttt
     1801 atttagtaaa acgtcacgtg ctaataccac gttcacgttt tccattaaag taagcagcaa
     1861 ctcgtattct ctttttgtta gttcaattac ttcatttccg cgacgtacga cacggttttc
     1921 tttttcgata gtcaaatcgc gataagtaat agtagtttgt ttagccacat ttttatcgcc
     1981 ttcaatatcg attcgacgca acaatgcacg aagacgtgct aataattctt cgatagcaaa
     2041 tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca gatacacgat cgatcacaga
     2101 atctcttgca gtcatcataa tgattggtgt atttttgact tggcgtacac gtctgcagac
     2161 ttctaaaccg ttcaattctg ggagcatcag atccaacaat atggcatccc actcattatt
     2221 taatgccgca tctaaaccag ttcggccgtt gtagtggact tctgcgttat acccttcatg
     2281 cttcaactct agctcgacaa atcgtgccaa gttcttttca tcttcaatga ttagaatatt
     2341 gctcatttat tattttatcc tttctattct actggctttt taaaaactct atcattatac
     2401 cgataatatg cttaaaaatc tatagtcatt ttatcatgga tagcaatttt aaaaaagagc
     2461 tgaagtctac tcataacaat cgacttcagc tctttcgtcc tctttttatt ttctcatttc
     2521 ctaacagccg aaagactatt attcttcatg gtaccatgag taatggaaga ttccttcttt
     2581 gtcgacacgc tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc
     2641 aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac
     2701 aggtacgcca gcttggacag ccaaggcaac gacgtctctg actgattgct gatatttttt
     2761 cgtgatttca acgaagtaat catctagcaa taaattttcg atctcaggat ttttgtcata
     2821 ggcatccgta attttttgca agaatcgagc tcggatgata catccagcgc gccagatttt
     2881 tgcgatttca ccaaatggca agtcccaatc aaattctttt gaagcgacac gtaattgagc
     2941 aaatccttgg gcataactca tgattttact gaaatacaac gcttctcgga ttttttcgac
     3001 caattctttt ttgtctcccg tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc
     3061 ttgtacgcgt tcttctttat aagcagaaat atagcgggca aatacagatt cagtgatcaa
     3121 tggaagtggc acacctaagt caagcgcgct ttggcttgtc catttacctg ttcctttatt
     3181 cccagctgca tcaaggatca catctactac tggtttacct gttccttcat cgtctttacg
     3241 tgtcagaatg tcagcagtga tttctatcaa atagctgtct aattcgcctt gattccattc
     3301 tttaaagata tcagccattt cttctactga aaggtttaaa atattcttca ttaagtcata
     3361 agattcagca atcagttgca tatctccata ctcaataccg ttgtgtacca ttttcacata
     3421 atgtccggct ccatttgggc caatatatgt gacacatggt gctccgtctt ctgcttttgc
     3481 agaaatttgc tctaagattg gtgcaactag cgcataggct tctttttgtc caccaggcat
     3541 gattgaaggt ccttttaaag ctccttcttc accaccggaa acgcctgtac cgataaagtt
     3601 gatgccagag ttggctaact cttcattacg acgcatcgta tctttaaaga atgtatttcc
     3661 tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagt aactcttgga ttgttgcatc
     3721 tgttgccggt cctgctttga ccattagcaa gatccgacgt ggtttctcga tagattctac
     3781 aaattcttcg attgtatatg ttgctttgaa atttttatct gggtgttctt ctactacagc
     3841 ggttgtttta gcccctgtac gattgaataa agccactgaa tagccgcggc tttctatgtt
     3901 caaggctaga tttttaccca taacggccat accgacaacg ccgatttgtt gttttgacat
     3961 atgaactcct cctacatacg ggatttctcc ctaatctctt acattatagc ttaacacggc
     4021 tacattgcaa actaaagaac aaca
//
LOCUS       DACCWV010000182         4034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCWV010000182 DACCWV010000000
VERSION     DACCWV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.187"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..627
                     /locus_tag="IVW84_002696"
     CDS             <1..627
                     /locus_tag="IVW84_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7918855.1"
                     /translation="GDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTS
                     ITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLIS
                     SVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIML
                     KSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            772..1077
                     /locus_tag="IVW84_002697"
     CDS             772..1077
                     /locus_tag="IVW84_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918856.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1694..>4034
                     /gene="esp"
                     /locus_tag="IVW84_002698"
     CDS             1694..>4034
                     /gene="esp"
                     /locus_tag="IVW84_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7918857.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTS"
BASE COUNT         1473 a          559 c          774 g         1228 t
ORIGIN      
        1 ggagatattc gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt
       61 gttagtggag atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta
      121 gcacaaacgt ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct
      181 ttgatacaag aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct
      241 gggattgttt attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta
      301 atttcttctg tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa
      361 atagctaatt atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg
      421 catattacga tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg
      481 aaatcaaacc atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac
      541 ttttcgagag tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta
      601 gtggataatt tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt
      661 acgtagtttg gaggagttga ggaaattgga ttttaacaaa aaattttttg ggaaatttct
      721 gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag tgtgttaaat
      781 gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc tagagaatct
      841 atcagtcata ttaatggtag caattttaag caaacattat ttaatctgat taagctagat
      901 gctaaaatat caagttattt attcttttta atacatgatg aatttatgga ttatcaaaaa
      961 ttggatcagt taattgaaag agaaagctgg gaagattatt acgtgggtct aactttttca
     1021 gaaaagttaa acaactacaa gttaatagat tataagtatt tgagtgaaag caattaatta
     1081 ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga aaaagaatca
     1141 ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa
     1201 tgttgataac tccaatcatt cgtttaagat tctaggaatc attcaggaat gctgtgaaac
     1261 cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac
     1321 ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt attccgctat
     1381 ttgaatactc tgaagtttaa tctaagaaag agtaatattt tgaaaacatg cttcatcgca
     1441 tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt tttcagctgc
     1501 tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact ttttttgttg
     1561 taatgtgcat gaaattcttt aagtaaacta gttaacataa gtttagtaag attcctattc
     1621 tgtgaataat acgtatcaga tttatctttt attcttacta agaaaataac tattaaggga
     1681 gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa aaacaaagaa
     1741 acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg ttttggttgg
     1801 tgttggtctt gtcttgggaa caactgaact agttcaagca caagatgaaa tttctccaag
     1861 caccccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag cttcggggaa
     1921 tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt tacaatttag
     1981 tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg aagtttacgg
     2041 aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg
     2101 attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg gatcttctag
     2161 acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt ggaaggtcaa
     2221 ttatattcgt gcaagtgatg gattatttgc tggggctgaa acaactgata cacaaactgc
     2281 tcctaatggg gtaattacgt ttgaaaagag cttagatgaa atttttaaag agataggcat
     2341 agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc atcaagacga
     2401 tgataaaatt gtaccaggta ttgatagtac gggctacttt ttaacagatt cagatgattt
     2461 ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg gatcagtaag
     2521 tgcaaaatat gaggaaccta atactcaaac aaaagatggt tcaggatcca caggtgctaa
     2581 tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat attcagcttc
     2641 cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag ttccatatgt
     2701 agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg tttcttttga
     2761 atcagggaaa aaagtggctg atttagcaat tgaacggcgc aaagatcatg aaaattatgg
     2821 tgtaggttca attactgata atgatcttac caaacttatt gattttgcta atgcaagtcc
     2881 acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg aaatcttaga
     2941 agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag atttcatctt
     3001 tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg gaacaggtta
     3061 ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag agagcggaga
     3121 cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg acacaacagt
     3181 taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc aacttgttga
     3241 taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag atggggctcc
     3301 aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa
     3361 agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag aaggttcaac
     3421 cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg gatttaaacc
     3481 agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag agtattcatg
     3541 gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg taactgttaa
     3601 gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt ctcaagctat
     3661 ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag ttcaaagtgg
     3721 aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt caattttaga
     3781 tgtctataat aaggacttcg gtaatgcaag tgttgatgca aatactggta ttgttacatt
     3841 cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc ctattaaaat
     3901 tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa gtaaagatat
     3961 ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtgacct tcaccgcagg
     4021 ggaaggaaca agta
//
LOCUS       DACCWV010000183         3969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCWV010000183 DACCWV010000000
VERSION     DACCWV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.188"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(121..579)
                     /locus_tag="IVW84_002699"
     CDS             complement(121..579)
                     /locus_tag="IVW84_002699"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7918858.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(750..1895)
                     /locus_tag="IVW84_002700"
     CDS             complement(750..1895)
                     /locus_tag="IVW84_002700"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7918859.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1908..2825)
                     /locus_tag="IVW84_002701"
     CDS             complement(1908..2825)
                     /locus_tag="IVW84_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7918860.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3253..3816
                     /gene="efp"
                     /locus_tag="IVW84_002702"
     CDS             3253..3816
                     /gene="efp"
                     /locus_tag="IVW84_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7918861.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1228 a          751 c          760 g         1230 t
ORIGIN      
        1 gctaaactga aaactagcta tttgttttcc cgtttagaac tcttttcaga caataaatat
       61 ttaataaaat acgaagataa gatagctata ttgcttctca tctttacgtt cttttagaaa
      121 ctatgcttct acttcagacc attcatcgcg ttttgctaaa aattcacgag ccaaagcttg
      181 tgcaccttct agtgtatggc tggcagccca gccacattgt gtttcattgc ttgcaggtac
      241 ttcagtagct tcaagaacat ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta
      301 gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg catcccattg gagaccagtc
      361 tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag
      421 acttggcatg tccatgtgtt ctttatttgg ttgtttaaag cggacgtcgt atttaatgat
      481 gacatcgcca tgcgcacccg tttttcgatc agctacacgt acataaggtg ctttgacttt
      541 tgtatggtca agattaaaac tttcgacatt cattctcatt tgtaagactc ctatctgttt
      601 gttcattcgt tttgatgctt ttcactaaag aattatttag tgaaatttac aaaaacgtct
      661 ttctacttga ttctaacact tatcgtttcc aaggtgtagc aatctgaaat attttttaga
      721 tttttctgct aagtcgttta ttgggacttc tagttcttga ttctgtcaaa tgcctgagct
      781 aaatcgttta tgagatcctg accatactca attcctacag aaagacggat caagccgtcc
      841 tttatgccag cctcttctcg tttttctttt ggaatagacg catgagtcat gacagaagga
      901 atttccacta aactttctac gccacctaaa ctttctgcta aagtaaatag ttggagtgat
      961 tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa aagaaatcat tccgctaaag
     1021 ccgctcatct gctgcttagc aactccatgg agttcatgat cgggtaatcc tggatagtag
     1081 accttttcta ctgctggatg agaaaataga aaatcagcta ccacaaaagc atttttttga
     1141 tgttcctcca tacgaacact tagtgtcttg attccgcgct gaagtaacca gctatcttgg
     1201 ggaccaagta cggctccgat agcattttgg taaaatccga tctgatcggc tagatcttta
     1261 tggttagaag tcactaaacc agctactaca tcgctgtgtc cgcctagata tttggtaccg
     1321 ctatggacaa cgatatcaga accgagatct aaaggtcttt gaaaataagg agtagcaaat
     1381 gtattgtcag caataactat caatccatgt tgatgagcca gagtggcact tttttctaaa
     1441 tcagtaatct ttagaagcgg attgctaggt gtttcaagat aaagtgcttt tgtattgggt
     1501 ttgatggatt gttctatttt atcaagatta ctcgtatcta tgatcgtgta ctcgagacca
     1561 tttttagtaa gaactttgtc aaatagacgg aatgttcctc catagacatc atctcctaat
     1621 aagatatgat cgccagcttg aaaaagagaa aaaacggcat gaatcccaga aagaccagaa
     1681 gaaaaagcga atcctcgaac accgccttcc aaatcagcaa tcagttcttc caatgcaaaa
     1741 cgagtaggat taccagatct tgaatattca tattgtttag gctggccgac gccatcttgc
     1801 cgataagtgg atgtttgata aataggtaca ctgacagctc ctgttgtggg atcttcactg
     1861 atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc tttcctccta taagtaaatg
     1921 ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt
     1981 ggtaaacgac gaatttctct taatgctgct gcaaaagccg caccactcga acttccaact
     2041 aatagtcctt gttctcgtgc taactgccta gtatagttaa acccttctac atcagagatt
     2101 gtctcgatct gattgattga aaggggagaa agaaacggcg gaatgaattc tacaccgata
     2161 ccctcaattt cgtgaggggc tggatcacca ccatttaaca cagagccctc tggttctaca
     2221 ccgattattc gaatgtcggg atatttttct ttcaaaaagg tagaagttcc agcaaaggtt
     2281 ccaccactcc caattcctgc tacaaaacta tggatatttt ctttgatttc atgaaagatt
     2341 tctggcccta acgtatgata gtaagcagca ggattatctt tattttcaaa ttgaagaggc
     2401 agatagctgt ttgatatctc ctctgcaagt tttttacttt tgctgatggc accgatgatt
     2461 ccttgctcac taggagaatg gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt
     2521 tctaagctaa acttctctgg aacaacaaaa accgttctta atttgtatgt gagtgcggcc
     2581 agagcgatcc caattcctgt atttccggca gttggttcga tgatcgtcgt tttttcattg
     2641 atttttcctt gggcaaatcc ttctttgatc aaataactgc ccaatctatc tttgatactt
     2701 cctccaggat ttaaatattc tagttttgca taaatagcag aacctttagg catagaatag
     2761 tgctgattgt caaagcgata aagcggtgtg gaaccaatag cttctgtaat tgtcgagata
     2821 atcatgtggg cctcctaaaa aaagataata aaaaaaggag ctgactatta aatcagctcc
     2881 ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa gttacctggt actcattcta
     2941 tgaagggcaa ttaatagaca tgacaaatag tatgctgctt ttatgcacca tacaacagca
     3001 acgattcaat tgtttttggt tgctgactgt catgtgtatc cctccaagta aaatttttat
     3061 taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat atttcgatag
     3121 catcgtttac ccgtgtttat ttttctgaca aataggcatg agaccttaaa agatagagaa
     3181 tactctagtt ttctggccgg ttgtctgata caataaagtt gattatgata aagaaaagaa
     3241 ggagcaaaaa taatgatctc agcaagtgat ttaagagccg gtatgacttt cgtacaagac
     3301 ggcaaactga ttaaagttct ggatgcaagc caccataaac ctggtaaagg aaatacagtt
     3361 atgcgtatga aactgaaaga tgtccgttca ggtgcaacat acgacacaac gttcagacca
     3421 gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg tacaatattt gtacacgatg
     3481 gatgatacag ctttcttcat ggatttggaa acatatgagc aatatgaaat tcctgtagaa
     3541 acagtagaag aagaaatgaa atatatccta gaaaacatgg aagtaaaaat ccaatttttc
     3601 ggtagcgaag tcatcggcgt tcaattacca acaactgtcg tattacgtgt tgtggaaaca
     3661 caaccttcca tcaaaggtgc aacagtcact ggttcaggta agccagcgac aatggaaact
     3721 ggcttagtag tcaatgttcc tgacttcatc gaaacagatg aattgttgga aatcaataca
     3781 gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg ctaacggatt gataagaatt
     3841 tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga tctgcctttc aagaggtttt
     3901 ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata ttttgaaaga
     3961 tgaatggca
//
LOCUS       DACCWV010000184         3870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCWV010000184 DACCWV010000000
VERSION     DACCWV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.189"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(143..1069)
                     /gene="glsA"
                     /locus_tag="IVW84_002703"
     CDS             complement(143..1069)
                     /gene="glsA"
                     /locus_tag="IVW84_002703"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7918862.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1092..2519)
                     /locus_tag="IVW84_002704"
     CDS             complement(1092..2519)
                     /locus_tag="IVW84_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7918863.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2550..3764)
                     /locus_tag="IVW84_002705"
     CDS             complement(2550..3764)
                     /locus_tag="IVW84_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7918864.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1221 a          841 c          703 g         1105 t
ORIGIN      
        1 tttccttttt atttagaaaa gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa
       61 gtcaaacaag ctcacaattt cagcctcatt gtaggtagct ttgagccgtt tggactttta
      121 ccccagcctc tttcaagaat ggttattcaa agatgtcagc gtctagttct ttgactacat
      181 ctttcaacaa ttggatacca gctgcgctgt taccaaatgg atctagtgcc gggctgaaga
      241 cacctatgcc gtaacgattt ggcacagctg ccatcaaccc gccgcctaca ccgctttttg
      301 caggtacacc aacatggacg gaaaactctc ctgattcatc atacaaacca gcagttgtca
      361 taattgattt gacgattgta gcactttctt cagtaatgat ttgtttgcca ttccaaggag
      421 caattccttt atttgccagc acagcagcta attttgctat gcctgtagcg gtgacattga
      481 gggagcattg tctgaaataa gcatcgagta tctccggtac gtctccttcg atcatttgat
      541 tacctttcat atagtaagca agagagcgat tgatatcacc tgttcttgat tcagaatgat
      601 agatttcttc attcaatgtg atattaggat catcacagat ctctttcata aatgacaaga
      661 ttcgctcaaa tttttcatct gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc
      721 ctgcattaac aaaaggattc attggatgtt tgcgatgatt gatttccata ttcatgatag
      781 aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg aaagactttt tctattccgt
      841 tgtctttgat tgctaataac aatacgggaa cttttgacat gctttcgatg gcaaagcgca
      901 catcggcgtc tcctccctca atcagttgtt ttgtatccaa atcataaatc gccataccta
      961 attgtttggg atccacttca gctaatgctg gaatgtaatc cgccacttgt cctgtttgat
     1021 aatagtggaa attttttcca atcaattgtt ctaattcttt tacttccatt tgaataacca
     1081 gctttcattt tttatttttc tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat
     1141 ggaataacta agacgataag ataagaaaca atcagaatcg tttcataaat cccatgctgt
     1201 ccagctgcga tactagcagg tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa
     1261 agacctaaac aagcaacgat tacttttaca acttttccgc ctggaacctg ataggctctt
     1321 tcaagactgt catctttaaa aaccagcttc aaatagcccg cgaaaaacat aaagtacaac
     1381 atcaggtaga ttacaacagt caatgacatc gctgcaagaa atgaaagatt attccccccg
     1441 ccgcttaatg taaagacggc tgcccagatt gttgcaacga tcccatttgc gatcatcaaa
     1501 tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa tccttcttct
     1561 gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc tccgatgaca
     1621 cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg atcaatgtgt
     1681 cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc atgttgaggt
     1741 actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag aataaccaat
     1801 acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc atgactggca
     1861 gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat
     1921 ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg agcgcctgaa
     1981 gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat accagctaca
     2041 aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga gagtaaaata
     2101 gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg tatagcacca
     2161 atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa gtagccaact
     2221 gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc caatgtgcga
     2281 ctgacccaag tatagacacc gcctttttcc catcctttca ccgttgccat ctctgctgcg
     2341 cacaatgcca cagggataaa ccatagcaaa ccgcctaata acaagaaaaa tactaagctg
     2401 aagcctgagg tcgcgaatgt gggatattca tataatgaaa cgaccattga gatggtcata
     2461 gccaaaaagc caaagaacgt taattttttt gcaggaattt tcgttttgct ttgttgcatg
     2521 aatattcctc ttttctattt gaatttgttt caattgagtt tgttgccaaa gaagaaaaag
     2581 tccaatcctt cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat
     2641 tttaaaatca ctgttgtcag cacataggca tacacggcag taatgatgac accaatcact
     2701 tgaatcccta agaagtacca gctgcttgga ttcgttagta aatggctgtt agatgcaaag
     2761 atccctatca gaatcgtacc tgtgaatccg cccatgccat gaacacccca cacatcaagt
     2821 gcatcatccc attgtttctt tttacgaaaa tcaacagcag catgacagac gatacccgca
     2881 atgatcccaa tcagcatggc actttttgct tctacataac cggcacaagg cgtaatggct
     2941 gccaatccag caacggatcc tgttagtacg tcgacaaaat caaagccatt tccttttact
     3001 tttgcaatga tcagccaagt aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat
     3061 gcattgactg cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca
     3121 aaccaaagca gtcctgtacc gatagcagct gccataagac tgctatgctg acttctttct
     3181 cctaaaggaa tctttctttg accaagtgtg aggatacatg ctaaagagcc gaatccagct
     3241 gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc ccatctgatc caaaaatccg
     3301 ccaccccata tccagtggca aacagggaaa taaatcagca gattccaaaa aataacaagt
     3361 ataatatagc cttttaaatt caatctccct gcaaaagctc ctgtcatcaa aggaacggta
     3421 atcacgataa acatcagttg gtaaagaaag aacattaaaa atggaatcgt tgcaccatga
     3481 aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg gtttccaata
     3541 acaccgccga tatcccgtcc aaaggccaat ccgaatccac caaataccca cataatcgtt
     3601 gttatcccaa cggagagaaa agattgagac atgatcgtta gtatattatt cttccgtgct
     3661 aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc tgtacataaa
     3721 atcatgaatg ctgtatctcc tgtattgata ggtaatgtat tcattttttt cgcctccttt
     3781 ctacgattac gagtataaaa gaaggtgtag ttgttttttt cttctgtttt tttgaattaa
     3841 tacttgcatt tataaaagac agacaattaa
//
LOCUS       DACCWV010000185         3868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCWV010000185 DACCWV010000000
VERSION     DACCWV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.190"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            113..1195
                     /locus_tag="IVW84_002706"
     CDS             113..1195
                     /locus_tag="IVW84_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7918865.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1195..1998
                     /locus_tag="IVW84_002707"
     CDS             1195..1998
                     /locus_tag="IVW84_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7918866.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2068..2808
                     /locus_tag="IVW84_002708"
     CDS             2068..2808
                     /locus_tag="IVW84_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7918867.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2819..3823
                     /locus_tag="IVW84_002709"
     CDS             2819..3823
                     /locus_tag="IVW84_002709"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7918868.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         1255 a          630 c          811 g         1172 t
ORIGIN      
        1 tttctttatt tttgaatatt ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa
       61 atcataagca atggtataat cacaatatga atatagaaat gggggaaaac atttggatac
      121 aaaagaagaa cagctactta ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga
      181 agaggtaaga gaagtattca gagaatatgc caaaccattc gctgatgata ttttttatga
      241 cggtttaggc agtgtgattg ccaaacatgg tgctggcgga ggccccaagg tctttatttc
      301 tggacatatg gacgaagttg gttttatggt aacaaaaata accgaaaaag gattcttgga
      361 attccaaact cttggaggtt ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa
      421 aacacgtgaa gggaaaatcg tccacggtgt gattggttcc aaaccaccac atgttttaac
      481 acctcaagta agaaataaac cttatgaaat aaaggatatg ttcattgata ttggcgcatc
      541 aagccaagaa gaagccaaag agtggggcat acgtcctgga gatatggtca ctccatatat
      601 tgaatacaaa cgaatgaatg gcagtaaata tttactggca aaagcatggg ataatcgtat
      661 cgggacagct gtttctctgc gagtattaga gaatctatct aaagaagcac acccaaatgt
      721 tttattcgca ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac
      781 acaccttgta aatcccgata ttgcttttgc tttagatact ggtacagcag gagacactcc
      841 aggaatgaca ccaaaagaag cagattcgat tttaggaaaa ggcccacaga ttttgatttt
      901 tgatgcatct atgatcccgc ataaaaaatt attgaatttt gttattgatg tagcagagga
      961 aatggatatt ccattccaat atacagtgat ttctggcgga ggaactgatg caggacaaat
     1021 gcatttgaca agagatggtg ttccgtctgt agccattacc gttcctgttc gttatctgca
     1081 ttcccataca tcagtgattc atgaagatga ttatttgaat acagtgaaac tagtcacaga
     1141 attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt cggagttatg aataatgaat
     1201 aatagaatac ctatttctga atatcggaaa aaagcacgag tttctttgga aaatcaatgg
     1261 ggaatcaata cttggcttgt tttcttatct ataatattta cgagcgtcat ccagaacttt
     1321 tttgacggtt tcagtccttt tccggaagaa agcatgcagc agcacttatt gagttttcta
     1381 gtcgataatt tacttttatt cgcattcaac tatgcattgt tttacattgc gctagtagtt
     1441 gtacggggtg gcagaccaaa aagtaacctt ttattttcag ttttccaaag aaagtatttt
     1501 ggttcgatac ttggcatcaa tttgatcaat tcagtcatca actggctact caacagttta
     1561 atctttctgc ccatgttttt aatcagcgga gtgagtactt atatgcagct gcttttcagt
     1621 tcaaatgcgg tacctacggg tgtcatgcca gaagattttc ttgatatcag ctttgtttta
     1681 tcgatgatag ggatgatttt tgtttcagca ctcatcatga gtattgttgg cggtttattc
     1741 caatttgctg catggacaaa gttcgactat cctgatttgt cggttattca atgtttgaaa
     1801 tatggctggt ttttattgaa agatagaatt ggtacgtata ttttgttgca gctgtctttt
     1861 atcggctggt atttgctagg agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt
     1921 tatgtgaatg tgtcagttgc agaattttat gaacaagcac ggattgaaaa aggaggtcca
     1981 gacgcctatt ttacttagca tgtaagcagt ttattcatca aaaaaataac ttttgtggta
     2041 ggataaatct ttgaggggga atgtacgatg aaattaactg ttttaggatg cttaggagcg
     2101 tatccttata aaaatcaagg gacaacaggt tatttgcttc aatctgacgg gttcaatttg
     2161 ttgatcgatg caggtagtgc tacgctgatc aaattacaag aacaccttga tccacttgat
     2221 ctagatgctg tgatccttag ccattatcat catgatcata ttgctgacct tggtgtatta
     2281 cagtattact ggcaacttca tcctaagaga aatccggaga cattgctttc aatttatgga
     2341 cacacaaaag attccattca ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat
     2401 ccttattttg aagctgaaga attaaaaatt ggtccttttc tgattacttt tatggaaaca
     2461 atccatccag tagtatgtta cgccatgcgt atcgtagaag aaacaacggg gaaagtattc
     2521 gtatttacag gtgattcagg ttatttggaa agtttcgctg attttgcgaa agatgctgat
     2581 ttgtttctag cagataccta tctttttgaa ggaaatgaac atcatcatgc ccatttcact
     2641 tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa agaaacttgt acttacacac
     2701 ctacctcagt ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa
     2761 gggattgata tagaacttgc tgcagttgac aaagtgttta acatttagga ggaagaacat
     2821 gatttttgta ccaaatgaaa accacgaccc aagaatcaat ttagcaattg aacaatattt
     2881 gctgcaagaa atgcctttag atgaacctat tttgctcttt tatatcaatg aaccttcaat
     2941 catcatcggc agaaaccaaa atacgcttga agaaatcaat cgagattatg tggaagaaaa
     3001 tggcattcat gttgtccgtc gcttaagtgg aggcggagct gtctaccatg attttggaaa
     3061 cctgaacttt agttttatca tgcctgatga tggggattca ttccgtgact ttgcgaaagt
     3121 aacacagcca atcatccaag cacttcatga attgggtgtt gctggggcgg aattgaaagg
     3181 ccgaaatgat ctagtcattg atggcatgaa attttctggc aacgcgatgt atgctaccaa
     3241 cggtcggatg tttgctcatg gaacaattat gttcgactct gatatcaatg aagtagtcaa
     3301 tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga tccgttctcg
     3361 tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt
     3421 tcgtcaagaa atcttgttga aaattttcgg cgtagagtca gtagaagaag taaaaacgta
     3481 cgaattgact gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa
     3541 ttgggattgg aattacggga agtctccaga tttcaatttc tctcgccaaa aacgtttttc
     3601 tatcggttcg attgaagtcc atttgaatgt ctcagaagga atgatcaaag aagcaaaaat
     3661 ctttggcgat ttcttcggac taggagatat ctctgacgtg gaaaaaaaat tggttggaca
     3721 gaaatatgat aaagcatctc tatcaaaaat agtcagccaa attgatgtga agaaatattt
     3781 tggtgcgata gaaccagaag aattgcttac cttactttat tgaaaataaa aaatagcgga
     3841 atccacatat atggattccg ctattttt
//
LOCUS       DACCWV010000186         3865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCWV010000186 DACCWV010000000
VERSION     DACCWV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.191"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(247..468)
                     /locus_tag="IVW84_002710"
     CDS             complement(247..468)
                     /locus_tag="IVW84_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918869.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="IVW84_002711"
     CDS             complement(566..811)
                     /locus_tag="IVW84_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918870.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="IVW84_002712"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="IVW84_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2035..3036)
                     /locus_tag="IVW84_002713"
     CDS             complement(2035..3036)
                     /locus_tag="IVW84_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7918871.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3195..3461)
                     /locus_tag="IVW84_002714"
     CDS             complement(3195..3461)
                     /locus_tag="IVW84_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918872.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3451..3807)
                     /locus_tag="IVW84_002715"
     CDS             complement(3451..3807)
                     /locus_tag="IVW84_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7918873.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1433 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     1981 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2041 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2101 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2161 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2221 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2281 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     2341 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
     2401 aaattctatt ggatcaaaaa tatctaagtt gtcatcaaaa tctttatcag ttccggtaac
     2461 ttttttgtta attactgaag gtgtcttgta agaaatagaa tctaaatcaa agcgttttga
     2521 ttctaaatta ttttgtgctt tctcaattaa ataattcact ttttttgtga gaaaccttct
     2581 gatattgtct ttgctatatg ttccataaaa acccttatct tctaaagagt ccctgtactt
     2641 cttaacttct atatactctt cagccaaaac ttgtggaact ggttcatcta agaaaaaaac
     2701 ccctacctcg cctttgctat ttttataaaa gtaatcaatt tctgctatag ttcccgatct
     2761 ataattatca actggagaac ccaacttatg gccaaaaatc gctaataata tatcactatt
     2821 ttctacaatt tgtttattaa taacactttg cccagagcta ttatttatat aagtaggaat
     2881 cgcattcgtt tcccatcgaa ttggcatcaa aattactttt gtattatctg tattttctat
     2941 attccatcta tatatggttt cttctacttc attcctcttt gctgctatat cacctggtga
     3001 tgctataaat acttttatta gatttatggt tttcatttat tgtacctttc tctttatgtc
     3061 tctttaaagt aatttttgta taaaatttat tgacttttac actataggac aacttttatc
     3121 tatttagtac aaaaaggccc actaaactaa ttgattagtt aggagcctat tacaggtata
     3181 ttttcaactc gcttttaagc aaattctact accagctttt tgcctaatgc cctagcgata
     3241 tcatcaagtt tatcgaaaga tacgttatcc cctctctcaa ttcgagcaat agtagattga
     3301 gttgtatgtg ctttttgtgc aagctccgct tgagttaggc ctgcttcctc ccgtgcatga
     3361 taaagagcaa ccgcagtttc gagttttctt ccttcacggt catatgcttc tgcaaaatct
     3421 ttgtcttttt gcttttcttc aatcaatttt ttaatcatca tttgtgaacc actcctctct
     3481 aagttctttt gcatgtttaa gttcacttat tggtgttttc tccgtttttt tagtaaatcc
     3541 atgggtaatg acataccttc cattgaccac atggaaatag atagctcttt ggatgtttga
     3601 tcctacctta cttcttaatt cgtacaaatt agtgtctaat tttttcaccc atttcaaccg
     3661 ttgagctact agcatcccat tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact
     3721 atcttttttt ggtaatgttt ccaaccattc taaaaattca tcatgaccat taggtctttt
     3781 aaatgtttca aaagatattt tttccatacc caattatatc atatattgat tttatattat
     3841 agtatataac ataaaaattc gaatt
//
LOCUS       DACCWV010000187         3860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCWV010000187 DACCWV010000000
VERSION     DACCWV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.192"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            89..1024
                     /locus_tag="IVW84_002716"
     CDS             89..1024
                     /locus_tag="IVW84_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7918874.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1109..2119)
                     /gene="gap"
                     /locus_tag="IVW84_002717"
     CDS             complement(1109..2119)
                     /gene="gap"
                     /locus_tag="IVW84_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7918875.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2407..3720
                     /gene="obgE"
                     /locus_tag="IVW84_002718"
     CDS             2407..3720
                     /gene="obgE"
                     /locus_tag="IVW84_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7918876.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         1213 a          685 c          877 g         1085 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa gaaagaagcc tttgactgga tccagtcaaa
     1081 ggcttctttt atcgattgat ctatcaaatt acaagttagc gaatttttct agtaagcgaa
     1141 tcatttggca agtaaatcca tactcattgt cataccatgc aacagtttta acaagttgga
     1201 aatcaccagc agttgttact tctgtttgcg ttgggtcaaa gatcgaacct tgagttgttc
     1261 caatgacatc gctagaaacg atttgacggt catcataccc gaatgaagga ttgtcaactg
     1321 tgtgtttttt gattgcttcg tttacttgat cagcagttac ttttgttttc aatactgaaa
     1381 ccaattcagt taatgaacca tcaacgactg gaacacgttg tgcgtgtcct tgtaattttc
     1441 cttgtagctc agggataact aagccgattg ctttcgcagc accagtagaa tgtgggatag
     1501 tattgtcagc agctgaacga gcagcacgca aattaccgcc ttttacaggt ccatctaaga
     1561 gcatttgtgt ggaagtgtag gcatgaactg ttgtcattgt accaacttcg atcccaaatt
     1621 cattatttaa gaaataagcc ataggtgcta aacagtttgt tgtacatgaa cctgctgaga
     1681 tgattttgtc gttttcatct aatgtatcat cattgacgtt atagacgatg gttttcatag
     1741 caccagctgg ggcagaaata actactcgtt tgacaccagc atctaaatgc gcttttgctt
     1801 tttcttcaga tgtataaaaa ccagtacatt ctagtacgat atctacaccg ttttcttttg
     1861 cccaagggat tttgcttgcg tcgggttctg cataaacacg agttgcttcg ccatccacta
     1921 cgattgagtc ttctgttgca gtaactgtgc caggataagt accatgagta gagtcaaatt
     1981 gcaataattg tgctaacata gttggacttg ttaagtcgtt gattgctact acttcaatat
     2041 catctgagac ttctttgatg cggcggaaag ccagacggcc aatacgccca aatccgttaa
     2101 tacctacttt tactgtcata tcaaaagttc ctcctttatt aggggtgctg tgaaacggca
     2161 tcctcaatta atacaattaa tacactgtta ttctgctacg caaagaatat tttgtcaaaa
     2221 aattatactt ggtagcagaa aaaaggttgg aaaccttacc aaatgataaa aaaagaagaa
     2281 ggtaaacaat ctttttgctg aaatgataga caaaatcaga tttcaccgat aaaataagta
     2341 agacttttgt ttattgtgga aattcaggca gtcaaataaa taaatgaata ataggaggac
     2401 ttaattatgt ccatgttttt agatcaagta acgatcgatg tgaaagctgg caagggcgga
     2461 gacggaatgg ttgcatttcg tcgcgaaaaa tacgttcctg acggcggacc ggctggcgga
     2521 gacggtggtc gtggcggcga tgtcatcttg atcgtcgatg aagggttacg tacattgatg
     2581 gattttcggt tcaatcgcca tttcaaagcg cagccaggtg aaaatgggat gagtaaaggg
     2641 atgcacggtc gtggttcaga agatacctat gttaaagtac cacaaggaac cactgtccga
     2701 gatgcggaga ctggtgctct tttaggagat ttaattgaaa atggtcaaac actagttgtg
     2761 gcaaaaggtg gacgcggtgg acgagggaat atccgttttg cctctcctcg gaatcctgca
     2821 ccggaaattg ctgaaaacgg tgaaccagga caagagcgaa aaatcgaact agaattgaaa
     2881 gtattggcag acgttggact tgtaggtttt ccatctgtcg ggaaatcgac tttactatcc
     2941 gttatttcat ctgctcgtcc aaaaatcggc gcttatcatt tcacgacgct cgtacctaat
     3001 ctaggaatgg ttactacaag tgatggcaga agttttgccg cagcagactt gccaggcttg
     3061 attgaaggag cttcgcaagg tgttgggctg ggtacacaat tccttcgtca tatcgaaaga
     3121 acacgagtaa ttttacatgt gatcgatatg agtgggatgg aaggacgaga tccttatgaa
     3181 gactatcttg ccatcaacaa agaattatct acttataact tacgcttgct agagcgtccg
     3241 caaatcatcg tggcaaacaa aatggatatg ccggatgcac cggaaaatct agtgaaattc
     3301 aaagaacagc tgaacaagga aaaagaagat gaatttgctg atgacatccc agtatttcca
     3361 atttctggag tgactcgaca aggattggac gcgttattga atgctacagc agatttgtta
     3421 gaagtaacgc cagaattccc attgtacgaa gaagaactgg aagaagaaac tgtacactat
     3481 ggattcaatc cagaaggtcc tgagttccaa atcgatcgtg attcagatgc aacgtggatc
     3541 ttatctggtg agaagatcga aaaattattc caaatgacga atttcgatca tgatgaaaca
     3601 gtgatgcgtt ttgctcgtca attacgtggt atgggtgtgg atgaagctct tcgggcacgc
     3661 ggtgcgaaag atggcgacct tgtacgtatc ggtgagtttg aatttgaatt tgtcgaatag
     3721 gcaagcaaat aaaaacaaat accaaaaaat atagattcag atttaaggga ggttgtgaca
     3781 gaagtgatca gcttcaagaa cgccgtttat tcggaaaaag gagctgtgat acgacttttg
     3841 tcacagccct attttattgg
//
LOCUS       DACCWV010000188         3747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCWV010000188 DACCWV010000000
VERSION     DACCWV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.193"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            82..360
                     /locus_tag="IVW84_002719"
                     /pseudo
     CDS             82..360
                     /locus_tag="IVW84_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            503..724
                     /locus_tag="IVW84_002720"
     CDS             503..724
                     /locus_tag="IVW84_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918877.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            740..1582
                     /locus_tag="IVW84_002721"
     CDS             740..1582
                     /locus_tag="IVW84_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918878.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1645..3447
                     /locus_tag="IVW84_002722"
     CDS             1645..3447
                     /locus_tag="IVW84_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP7918879.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3506..>3747)
                     /locus_tag="IVW84_002723"
     CDS             complement(3506..>3747)
                     /locus_tag="IVW84_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7918880.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1330 a          537 c          754 g         1126 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaagaactc atcaagctgg ctaataaggg tcaagattat
      121 gaacatctag cagatgagat tgatgagctg agagacaagc gacagctcct tttagtagaa
      181 gatgcctccc tcagtggcga gaacgagcga atcaatgagc tgattgaatt tatccgcaag
      241 aacaaattcc gtaccttaga gtacgatgat aagcttgtaa ggaagataat ccagagcgtt
      301 acagtctatg aagaacactt cgtcatatcc ttcaaatctg gcatcgaaat ggaaatatga
      361 aaaccagaag taaatagccc atgattctac agtagagtcg tgggttttct tttgcttgct
      421 gattataata atattttaat aactattgtt attataaacc aaatgattta taatattctt
      481 agcgtaaact tggaggtgct gtatgactac ggcagaaatg attaaagaac tgtgtgagca
      541 aatgaatata agtgtttccg aacttgctag acgtattgga cagactccac agaatttcaa
      601 taagaaatta caacgagaaa cggtaacctt agatgagttg aaagcgattg ctgatgtgct
      661 aggtgtcaag tttgtgcagg cttttatatt gcctgatggc aatgaaataa agataggtaa
      721 ttaggaggtg taatttgtat tgaaaatcat aacaataaat gatgttgagt atgctgtatt
      781 tgctgctaat gaaggtactt ccaaaccaca acctcatatc atagaaacga aatctggtac
      841 tattccagaa ggaaaacaac tgagtttatt aaaagaatat cttaagcaga atgatatttc
      901 cccaataaaa ggagcaacaa catattggtg cattgacaag gttcttaagc ttgattcaag
      961 taaagaaaaa actataagtg aaacaatcca taaacaaaaa tacttatctt taactgaaga
     1021 aaatattgag aaacagcata agtttgtagg tgcaagctct aattatggga aagaaggtct
     1081 aataatacat gatgttttga atgcatttcc tcttcataat gatttaaata ctattgcgat
     1141 gaagattgcg gtaattgatg taacaaattc aacacatctt tctcaatata agagcaggtt
     1201 gtctctatat gatttggcta aagtaattct ggagattcca aattttgatg atcggttggc
     1261 aaaaggagat cctcagttga taaatattat cgcaaggaac attggtgcag ttaatatgtt
     1321 ctcatttgca tccaagtact gtacatatca taatgtggaa gtgtgtggga gagatgatta
     1381 ctccatcttt gatggtatcg ttaaaaatac tcttccacac tatatccaag gactaactac
     1441 gaataagatt gatacgtggc gaagaagttt tgattacgag gcatttaacg aatgtgttgg
     1501 aaaactactt gatgaaaata atatacatat tccttttaga agaagaaagt tagatcactt
     1561 cttgtggtat gcgaataggt agaatataag aaaaaggcat cgtaaaagtg attctggata
     1621 aaattaatgg gggaggaaat aatagtggga ttatttgatt ttctaaagaa aaaacaagga
     1681 aaggttagta aacacgttga ttatcaactg gcttctagtc tatttcgtac tggtaaagtt
     1741 tttgctgaaa aattggaagt ggacatatca aatgtagatg agtttcgtaa aacttttatc
     1801 gcttttgatg tggaaacaac tggcctaaat tcttcttcag atagaattgt agagattggt
     1861 gcggtgctat ttaaagaagg agttccaatt caatcattcg gaactcttgt caatccaaaa
     1921 gttagaattg cagcctcagc ttcggcaata aataatataa ctaatgaaat gctaaactct
     1981 gctccaaatg agcaggaagt ttacccccag ttggtagaat ttttaggaga tgcactagat
     2041 ggaaaaatca ctatgtgtgc acacaatgca agtttcgatt ttggattttt aagtaacaca
     2101 ttttctagac ttgggtatga tgctaaaatt aagtatgttg atactctgag tctctctaga
     2161 aaacatataa aaggcattgt aaatcataag caagagactg tagcggaata tttcggatta
     2221 ataaatgaca ctgcccatag agcggaatca gatgcagata tatgcggaaa gattttttgg
     2281 ggtgttttag atattattga ggagtcgttg gaagaacaaa aaaagcaaat agaaaagatg
     2341 actccagaca taaaagaact tgaagcttgt gcttttattc aagatattat tgagaaaaaa
     2401 ggatgcgaca ccagttggat caggtatcga aaaaacagta ataattatgt cgatgtaact
     2461 tgtctatata cattcttaaa gtttaagttt gctaagaagg gaaattacat cattattaga
     2521 aaagatgcta caaatggaat tgatttacct atagaacctt gtactgtaag tgaaggcggt
     2581 acaacaaatg tgcgagtcta ttttcgtagc ccctttgact taacgcctct ttcaaattat
     2641 attttcagtg cttatagcga ttgttataat tctatgaatg aatatatgag tgttagtaat
     2701 tacactaaaa aagaagcaga gcaaagcctt agtatgctga aatctatttc aaaatcagcg
     2761 atgaaagagt tattattcga tgcaaaaaat cgtggatatg atgagactga agttaaccta
     2821 aatattgaaa aagtgatatc tagagaagat gtaattatta acgggttgca taatcgagtt
     2881 tcattaaaca agatacgaaa tatagggaat tgggagaaag gatttgatgc aggttatcca
     2941 tattgggaac gtggagaaac tgctaggaaa aatggcaaag tagatgaagc tattgcactt
     3001 tttgataaag ctcgatataa cggctatgat gctcctgcgc tatatgattc ttatgctaaa
     3061 gcttatcgaa aaattaagga ttacgataat gaaattctca ttcttgatga aggaattatg
     3121 cgtaaaacac ggcacgatgt tggtacttta gaggctcgta gggataaggc aattaagcta
     3181 ttatttgcta aacaagaggc agagcggata gctaaggaaa aatctgattt tctaaaagcg
     3241 aacaaaaaag aggatgcgat tagtgagcct agcaagaagc gtggaagaca gattattcaa
     3301 atgactgatg atggaacaat aataaaagaa ttcgatacta tagcttctgc tgtaaaagaa
     3361 gttggcgtta actcaaaaag tattagggat gctgcaaatg gagttcaaaa acatgctgga
     3421 ggatactact ggaaatacaa agattagcag ataatctaag ataacctagg atcttcttaa
     3481 tagatacccg tggtgctagt taattttaat atcctaaaga atatctcaaa gttccttcaa
     3541 aacgtcgttg tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac
     3601 tctctagccc tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag
     3661 aaaaaacagt cccaactgtt tttctttgtt ttccaagtaa gctgtggtct agtttttctg
     3721 atttatccat gtttttacgt ggcggaa
//
LOCUS       DACCWV010000189         3673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCWV010000189 DACCWV010000000
VERSION     DACCWV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.194"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..327)
                     /gene="rpmA"
                     /locus_tag="IVW84_002724"
     CDS             complement(34..327)
                     /gene="rpmA"
                     /locus_tag="IVW84_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7918881.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(340..684)
                     /locus_tag="IVW84_002725"
     CDS             complement(340..684)
                     /locus_tag="IVW84_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7918882.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(711..1019)
                     /gene="rplU"
                     /locus_tag="IVW84_002726"
     CDS             complement(711..1019)
                     /gene="rplU"
                     /locus_tag="IVW84_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7918883.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1301..1942
                     /locus_tag="IVW84_002727"
     CDS             1301..1942
                     /locus_tag="IVW84_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7918884.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2004..3635)
                     /locus_tag="IVW84_002728"
     CDS             complement(2004..3635)
                     /locus_tag="IVW84_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7918885.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1118 a          820 c          652 g         1083 t
ORIGIN      
        1 tcttgtcaca acctctttaa tcataagatt caattaagct tcttgagcta ctggataaac
       61 agacacttgt tttttgttac ggcctttacg ttcgaaacgt actacgccat caacttttgc
      121 aaataaagtg tcgtctccac cgatacccac gtttacacct ggatagattt ttgtaccgcg
      181 ttgacggtaa agaattgatc cacctgtaac tgtttgtcca tcagcacgtt tagcacctag
      241 acgttttgat tctgagtcac ggccgttaga tgtagaaccg ccaccttttt tgtgggcaaa
      301 taattgtaga ttcatagata acaacatggt ctgcacctcc tatttatcat taattgtttt
      361 aatttggata tattctgagt tttcagcttc tatcgattgt aatcctaata aaagattttc
      421 taatagaatc tcagcaatat tgttttgttc ttgtgtcatt cctgaagtga cttcggtata
      481 aagataaccg ccttcttctt cattcatttc aacaatcggc tcaaaaccgg ctaatgaggc
      541 aatcccgttg acagtgctga tcgctaacgc agaaacaccc gcacaaacga tatcacttcc
      601 ataaggacca gcatcagcat gcccggtcaa agtgaatgaa acgatctgac ctgcgtcatt
      661 tcgcttaaat gttcctttga tcatatatag gccttctttc tcaaacgcta ttaagcgttg
      721 atcgcgtcga taaccacttt tgtatatggt tgacgatgac cttgtttacg gtgtgaatgt
      781 tttttaggtt tgtatttgaa agtaacgact ttcttttgtt tgccgtgttt ttcaacagta
      841 ccttcaacag ttgcacctgc aacagttgga gcgcctactt ttgtagattc tccgcctact
      901 aagataactt cgtcaaaaac aactttttct cctgcttcaa cgtcaagttt ttcaacatag
      961 attgcttgtc ccacttcaac ttttacttgt ttaccaccag ttttaataat tgcgtacatg
     1021 ctataaagca cctccttctg atatacttag actcgccatc cgaagtgaca gcatatgctg
     1081 tgcttattac ttgttctgag cggttgtagc tgtggagtcc acaattacaa cagtactagc
     1141 ttaccagaac agcaaccgtt agtcaactaa atttaaaatc aaaaagtaaa acaaaaattc
     1201 aagcaatact tctgcttttc tatcctcaca gttctaccaa ataaaatagt cgcattgtgt
     1261 ttcttttatt cttataataa aagaaaaagg aggagcataa atggaatacc caaaagaact
     1321 gatacaagaa gcttctttaa gaatgaacgg ccgtcctgta gaaggattta tcgctgggca
     1381 aggacctctt catcctaaac tgatgctcgt gggagaagcc ccagggaaaa cagagattga
     1441 tacgcatgtt ccttttagcg gtcaagctgg aaaagaattg atgcaggcac tttcttcaac
     1501 tggcttaaca cgagaagagg tgtatatcac cagtgctgta aggagccgcc cgtatcgcgt
     1561 gactcatcgg atcaataaac ggacacaaca gtcagaaact gtttatccaa atcggacacc
     1621 tactcgttcc gaagtatttg cgcatgcacc aatattggat tacgaactga tgaaggtcca
     1681 gccaaaatta attgcgacac ttggaaacat tggtcttcaa cgtttacttg gcaaagaata
     1741 tacggttacc aaaaatcatg gtcaactcta tacagggcct gtcttacaat taacagaaca
     1801 aaaggatgcc tacgaatggt cagaaaaaaa ctatcgaatt cttccacttt ttcatccagc
     1861 agctattttc tacaatcgct cccttgtacc tgttatccat caagactggc aagtactagc
     1921 ttcactttta cagcaatatt aaaaagaaca gacaagacca taaaatcttc agtttacagt
     1981 cttgtctgtt ctttttcatt gttttaatgg ctaattgcag tatatttcgc atgtaaagct
     2041 gtcaacgcat gagcaactgt ttgatgttga tgcgtgaatg tatgttccca caagtgagaa
     2101 agttgttttt ctacttctgg ctgcaaggaa gtatagataa tcgtttgtcc tctttgctct
     2161 gctttttctt gaaattcatc aaaaactgca cgtgccgtca aatcaatact tggtacgcct
     2221 cgcatggaga aaatgacacc atcgtgatga tttagttctt ccaatttatt tttgagtttt
     2281 tctgaagaca taaaaaacag tggaccacta atatacacta ctgtccagtt attcagcttt
     2341 tccgtttctg gcagccccat tctttgccaa tcgatcgttt ctaatgaaat cgtgatagca
     2401 gcgcttttga caacaaaaaa tacacagccg ccgatcaccc cgatcacgat agcaatactt
     2461 aaatcaaaga taactgtaca aatcatcgta aggataaaca atagaactgc tgaccaatat
     2521 ttattagtaa atagctcctt gatcgtttcc cattcattca tacgccaagc cgtcacaatc
     2581 aaaacacctg caagtgctgg cattggaatc tgtgacatca ctggcgcaaa aacaagcatg
     2641 gagagcagta agacaactgc atgtatcata ccagctaatc tagtttgtgc accagatttg
     2701 atggccacgc ttgtacgggc gatggccgca gtagcaggaa tcccgccaaa gaaaggaagc
     2761 aacagatttc caattccttg cgccattaat tcttgattgc tatctaaggg cttatttgcc
     2821 attcggccag ccgaagcacc acatagcaag ctttcaatca ttcctaataa agcaatgcta
     2881 atagctggaa ctgccacttg ttgcaaggca gaaaagctaa aatctcccat attcaaacga
     2941 ttactactga tcaatgtttg tggaatctta cccacagttg ctactggtaa atggaacaat
     3001 agcataactg ctgttgccaa aatgatagcc agcagcgaac taggcattcg tttcgcccat
     3061 ttttttggat agataaacat ccctaaaatg actagccctc ccatgatcaa agaggctgtg
     3121 tttatcggaa atcctatcgt ttgataacta cttagttttt caataacgtt tgtaccttcc
     3181 gagtgaacac caaataaatt atcgatctga cctaatgcaa taatgatagc aatacctgaa
     3241 gtaaaccccg taattacggg tgctgggata aaagaagtca gtgttcctaa tcggaaaagt
     3301 cctgctgcaa gtaaaaatag acccgctaaa aaagtagcaa gcaatacacc ttgcattccc
     3361 tttgttgccg caatagacat caagatagct gccatcgcac ctgtcggacc tgatatttga
     3421 taaaaaccgc ctgataaacc gccaataacg ataccggcaa tgatagccgt aatcaaacct
     3481 gcggccgcat cagctccgct tgatacccca aaagccaagg ctaaaggcaa tgctacagca
     3541 gctactgtga tcccagccaa aagatctttc tgaaatcttg aaagatcata acctgaaaat
     3601 tcttttttta gtgaggaaac ataatttttg aacacctatc taactccttt ttcatgaaat
     3661 tttctaaaat acc
//
LOCUS       DACCWV010000190         3650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCWV010000190 DACCWV010000000
VERSION     DACCWV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.195"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            466..774
                     /locus_tag="IVW84_002729"
     CDS             466..774
                     /locus_tag="IVW84_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7918886.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            801..1100
                     /locus_tag="IVW84_002730"
     CDS             801..1100
                     /locus_tag="IVW84_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP7918887.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            1274..1825
                     /locus_tag="IVW84_002731"
     CDS             1274..1825
                     /locus_tag="IVW84_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP7918888.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1971..2264
                     /locus_tag="IVW84_002732"
     CDS             1971..2264
                     /locus_tag="IVW84_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918889.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            2257..2553
                     /locus_tag="IVW84_002733"
     CDS             2257..2553
                     /locus_tag="IVW84_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918890.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2628..3626
                     /locus_tag="IVW84_002734"
     CDS             2628..3626
                     /locus_tag="IVW84_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7918891.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1377 a          498 c          698 g         1077 t
ORIGIN      
        1 aagaatcaga tgggttttta ggaataatag atgagccact taacttctgt ggaagtactt
       61 tcgctaattt attaagatgt tccaaatcaa gagaatgaaa aacatcttcc ataaaactta
      121 tagttaaagc tttcaatttt gacttccttt cttgtattat ttataataac aatagtagta
      181 taacattaat aggatagttc attaagcaaa attaagtatc ttagttacta ataactatat
      241 aaattttaaa cttgtagtcg caatttagta tagaggtact aaatatgaaa actccatact
      301 tttagaaaag catttcttat atttagttgt gatgtctatt cagttaattg aaataaataa
      361 tatagcaatg aaatagagat aaattcaaat atatattata tccatgaagt ccaatcttct
      421 ttatagaaga ttgggctatt ttttacgaca aaggagggaa aagaaatgtt tcaaaataaa
      481 ggacaacgtt atgtcacaaa gggagtaatg gacagtttac tggtggaatt acaaacttta
      541 tgttggaatc taatcgatca aaacgttcaa aagaagcttc ctttagacta tcttcaaatc
      601 tttgaatttt cgatagaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccggaa
      661 gcaagaaaag agtatctgat ttcacctaag cttcgtttgg aatcagtgtc tcaaaaaata
      721 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      781 aaagaaagga gcgaaatgac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      841 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      901 ttgaaatggg tacgcttcct aaaaatgttc atctaccacg tttagtgatg acacatcttt
      961 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
     1021 ccagccagcg agaatttgtt tgtgggttac tggtagaagg acgattggtt tggagtcaga
     1081 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
     1141 atccttaccg cgatttatcg aattctgaag aaaaaaggag aaagaatatg aaatattttc
     1201 aactagtatt gacacttata acggcaatgg taattgaatt gcttgaacaa aaattagcat
     1261 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
     1321 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcaatcaaag gaaaagaaga
     1381 gaaagaaaaa ttagcagtag tcattaccat tcgtccaaca gatacagaac catttacgaa
     1441 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttgggattta ctggttttat
     1501 tgccgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttt ctcgtggcaa
     1561 tgatgaacaa tctttggaaa tattaacgac catttttaca gaaaagaaaa tcaacactat
     1621 tatttttgct tgtggttcga ttacggcaac gagtcaagtg gcttatgaac aagcaaagac
     1681 catttacgat caattgagtg cgaaacaaaa gaagatgacc aaagtattga taaatgcaga
     1741 aggcaagtta tctcacccat taagtattca tgttcgtaat gaatggcaat tggcagatag
     1801 tcaattatta tttcaagaaa aataacattt tattttctca tgttacttat aagtagtttt
     1861 acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat tatcaatgta
     1921 cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag atggagattg
     1981 atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag gaaatggaac
     2041 tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag atttcttttc
     2101 caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg atagaacagg
     2161 aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca agatggattc
     2221 aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat tgtattaggt
     2281 ggtttaattt acggtggata ttggttatat caatatatgc ttgcgcatcc gaatatagat
     2341 aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc tctaactaag
     2401 aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg atgtggaagg
     2461 gaaatttcta ctagttttaa tccatctgag agtcctcaag gaagagaccg ctgccatacg
     2521 ggctatcgac attattggga aagaatcgat taaaagatta ccatttagat caattaattg
     2581 tctgatagaa taagtacgtg agggagaaat tgaaaggggg aaatataatg aaaaaatggc
     2641 tgttattact aataggaatg ggaattttat tcactggttg tacatctgag aatacgagga
     2701 ctaaagcgac tatagaaact gatacaacaa ttgagactaa aaaaagtaaa acgcaatcaa
     2761 ctgaacaaac tacaacagaa atgaagcaat ctgtggaaac aagtgcaagt tcaatcactg
     2821 aatcttcttc tgaagaggtt aagaatactt tgtgggatac gaataaagct tccaaattag
     2881 aaacttttgt cacacaatgg ggaaagacat taggtcaaga atataaaagc tacaatcttc
     2941 agaataatgt aagtctgtat ggcactcctt taccacaagc cgtgattaat ggcgattgga
     3001 aaatggcaat caatgaagca ccagttaccg tacaatggtc agaagatgga actggtcatg
     3061 cagatttcaa tttagtagca gtttattctg atgtagaaac tggagaatat ttagggcagc
     3121 atgtttactt tttcggtttt caaaatggac aacctaaagt ttatttaacg caacaaaatc
     3181 aaggtaatga aaataattat ctgtacttta acgaaactca aaatcaacag ttaaaacaag
     3241 gtttcagtga tattgtgaat ggacaaactc ctcaaacacc agtagtagaa gaatctactc
     3301 aacaagcaac aactacaacg acacaatcta caagtagttc aggaatttca agttgggaag
     3361 ctgctaaaga atctgtgcta aataataaag ctttatggtt gtatgattct cctgataaaa
     3421 atgtatcaaa tactaattta attagcacaa atgaatatat gaaagatcta atgcaagatg
     3481 aaaatggaag ctactattca attaatattg taacggatga tgatggaaac acgaccatgg
     3541 ctggtggaaa acaatttcgt gtttatacag atggaagaat ttctcaacgt attggaatga
     3601 ctaacaattt tcaaatgatt aaataattga aaatattatt tatcaaagaa
//
LOCUS       DACCWV010000191         3649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCWV010000191 DACCWV010000000
VERSION     DACCWV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.196"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            930..1934
                     /locus_tag="IVW84_002735"
     CDS             930..1934
                     /locus_tag="IVW84_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7918892.1"
                     /translation="MLGDSLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFW
                     EDDSDLLGYADTVNSVYRPGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQF
                     LSVLEMEPNTRKIKLYNEDLENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQFSDK
                     VLDRSNIISLELVPFHEISEVNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKE
                     MFWKLHLAINSKDKNIGIGWRILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVLSKIR
                     GSDEQLSELVGKWNENDLKQVGEFEQIINEYPESSDFKYTKKILLQKARELNLHGFTL
                     "
     gene            1918..>3649
                     /locus_tag="IVW84_002736"
     CDS             1918..>3649
                     /locus_tag="IVW84_002736"
                     /inference="COORDINATES: protein motif:HMM:NF016309.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918893.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKRGLLYTPSRIIKYDIIENQWIKFILSYFKNFSLLADRYLEKILQGI
                     IEEKKREDKFTSAYSQSVQNYNTNSYNRKIENLKNNQQKIKHFYAYILEFLDTPVMKN
                     ISSRRPINVPKSLVMNPKYNTIYKMYLKSRNNRNTLNFSNRYQYYWKSTDVLYEIWCF
                     IKIIRSLSLQGYTPISGWIFENDILSQELPFLHEDTTVVLSKNDIRINLVYNNSMEKE
                     YFSNTLDIPVKTSSKRNKPDIRLDIILQDKYYIGSLIFEVKYKKLDNILDNDKGRQRQ
                     QLMAYKQNTMSSILAFPEILTRSLQAVSAVFALYPSNEGRKKTAPKYYEKEGIFFHLL
                     NPSSDEKELSV"
BASE COUNT         1454 a          506 c          539 g         1150 t
ORIGIN      
        1 ataaaattaa aataaactcg gatgatgtaa tgtacttttt tatgactcct ctttcagatt
       61 tccccagtag tgtgattgat aatgtagagg gtatacaaat atatagtaat acgcttacta
      121 aatttgggtt tattgatgat ataaacgaat ctcatagtac tcgtgcccaa tcacttaaag
      181 aatttttaga tccttatatt atcgtttgta caccaaaaat taaaagaaat catgaacaag
      241 caagaaatta ctatcaaggt gatgatttag aattaatccc caaaccaaag tctgaagttt
      301 ttaaaaattt aataaaagag aatgaatcat tagtacctat accggtcttt tctataaata
      361 acagcgattc tgtaatcaaa gacgagacaa gttttgaaaa ccaattacta aacggcaaac
      421 tactcggaaa aattataaat ttctcaaaag acaatgaaga ttacccttta aatgtttttt
      481 gggagaacac tgatggatca agagttttat ttggtgaaat aatcggacaa aacaattcaa
      541 tgtacggtgt agcatatagg atagaaaaag ataattttta caaaaagttt attgatgaag
      601 gtagtaaaaa ctatgtaggt tacgatgatt ttataaatca aacaaaagat ataatttttg
      661 tgccctatct attacttcaa aaaattatag aaactaaaaa agatattaac gaaggcatag
      721 aaaatatctc tgattccaaa atatctccag taagttcaat tattactgaa caaacgcaaa
      781 tagatatatc tgagttagaa gataaacacg ctattcaaca aaatgatcac ttctctgaac
      841 ataatgaatt tatagaaaga tttaatcagc tcacaattcg tagtaaatta cattactcaa
      901 aacgagactt atggaatttt catactgcta tgttgggtga tagtttggtg gtattagctg
      961 gactaagtgg taccggtaaa tcacaattag taacatccta tgcaaaagca cttcaattat
     1021 cagatacaca aataaagttt atttctgtga gacccttttg ggaggatgat tctgatttat
     1081 tgggctatgc tgatactgta aattctgtct atcgtccagg agatagcggt ctaattgatg
     1141 cactattaga agcatcaaga cataaagaaa atatttatat ggtttgtttt gatgagatga
     1201 atttagctcg ggttgaacat tatttctccc aatttctttc agttttagaa atggagccaa
     1261 atactaggaa aatcaaactg tataacgaag atctagaaaa taaattatat aactcttcaa
     1321 cctatccttc tacaattgaa gtaggacaaa acatattatt tgtaggaaca ataaatacag
     1381 atgaatctac tcatcaattc tcagataaag ttttggatag atcaaacatt atttcacttg
     1441 agttagtacc attccatgaa attagtgaag taaatgacta tattcaatca gatcaaaaga
     1501 aacgtaatca acttaaatat gatgatttca aaaattttaa aaactctaac cctactaatg
     1561 gtttgaccca gaaagaaaaa gaaatgtttt ggaaactcca tttagcaatt aattctaaag
     1621 acaaaaatat cggtatcggt tggagaatat taaaacaaat tgatgaatat ttaaaaaatc
     1681 ttccagtgca aaatgactta tcaagaaaag aagctattga tattcagctt gtacagagag
     1741 tattaagtaa aatcagaggt tctgatgaac aattatcaga attagttggt aaatggaatg
     1801 agaatgattt aaaacaagtg ggtgaatttg aacaaataat taatgaatat cctgagtctt
     1861 cagattttaa atatactaaa aaaattcttt tgcaaaaagc tagggagttg aatttacatg
     1921 gatttaccct ttagtctgaa attaaacttt ggttataaaa gtgaagaaca agaatatgaa
     1981 cttttatttt ttcaagatga ggcatcgtta ttagagtata caccggttaa ggtaaaagaa
     2041 ttttctaatc taagctttac atttaacagt atagacaaac acgcaaaact atatatacca
     2101 gaattagatg attatttgat tgaaaatcaa gtaaaagagg aaaatggaga tatttgctat
     2161 attccaagtg atgataatat aatcatttat aatagggatc aaggtaaaac agtacctctt
     2221 attccaggtt attacactct ttacgttaaa ataggtgaaa ctatctttta ttctcgattt
     2281 gaaataatac ttaaagattt aggtacacca gaatgggaaa aaatgaaaat cgaaattgaa
     2341 aaaatggttg gcggtcttgc tagaactttt attcctaaaa aaacttctcg tcgtgaaatt
     2401 actacttcaa attatctaat gaacgatcaa ctttttcaac gaatattaga gtttgagaac
     2461 aatatacctc aagcaatagt ttctcttgaa agtttaaaaa aagaagcaaa atatagaatt
     2521 gacaaacgat ataactggaa accaattgga agcaaaaatt tagtggatca aaaaactatt
     2581 tcacagttgc aaatccatcc agaaaaacgt gggctactat atacacctag ccgcataata
     2641 aaatatgata ttattgaaaa tcaatggatc aaatttatct tatcttattt taaaaatttt
     2701 tcacttctag ctgatagata tttagagaaa atcctacaag gtattattga agagaaaaaa
     2761 agagaagata agtttactag cgcgtattct caaagtgtac aaaactacaa tacaaatagc
     2821 tataatagaa agattgaaaa tttgaaaaac aatcagcaaa agataaaaca tttttatgct
     2881 tatatccttg aatttttaga cacaccagta atgaaaaaca tttcatcaag aagacctata
     2941 aatgttccaa aatcacttgt tatgaaccct aaatataaca ctatatataa aatgtattta
     3001 aaaagtagaa acaatcgtaa tactttaaat ttttcaaata gatatcagta ttattggaaa
     3061 agtacagatg tactctatga aatttggtgc tttataaaaa ttattagatc tctatccctg
     3121 caaggctaca ctccaatttc tggatggatt tttgagaatg atattctttc acaagaattg
     3181 ccgtttttac atgaagacac taccgtagta ttgagtaaaa atgatatacg tattaatctt
     3241 gtatataata attccatgga aaaagaatat ttctccaata cactagatat cccagtaaaa
     3301 acctcttcaa aacgaaataa acctgatatt agattagaca ttatccttca agataaatac
     3361 tatatcggaa gcttaatatt tgaagtaaaa tataaaaaac tagacaatat acttgataat
     3421 gataaaggta ggcaacgtca acaattaatg gcttataaac aaaatactat gtcaagtatt
     3481 ctagcatttc ctgaaattct tacacgttct ttgcaagctg tctctgctgt ctttgcttta
     3541 tatccttcca atgaaggaag aaaaaaaact gcacctaaat attatgaaaa agaaggtatt
     3601 ttttttcact tattaaatcc ttcatcagat gaaaaagagc tttcagtaa
//
LOCUS       DACCWV010000192         3613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCWV010000192 DACCWV010000000
VERSION     DACCWV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.197"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            194..1960
                     /locus_tag="IVW84_002737"
     CDS             194..1960
                     /locus_tag="IVW84_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7918894.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1975..2412
                     /locus_tag="IVW84_002738"
     CDS             1975..2412
                     /locus_tag="IVW84_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7918895.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2425..3576
                     /locus_tag="IVW84_002739"
     CDS             2425..3576
                     /locus_tag="IVW84_002739"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7918896.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1214 a          637 c          775 g          987 t
ORIGIN      
        1 gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt
       61 ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca aagtttcgtg
      121 ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc aaaaggagag
      181 agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa aagcgagggt
      241 acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag cactgattgc
      301 ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg aggctttggc
      361 taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt atactggcgg
      421 aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga tgggtgtgat
      481 tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc ttggtggata
      541 tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt ttgaaatgct
      601 agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag gtttttctgc
      661 gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag agatcatttt
      721 atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa tcttattttt
      781 gaataatgca atcaatcatg ggattttgac accattggga actgaacaag tagcagaggc
      841 tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg gcgtattact
      901 tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag ccatgatcat
      961 tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga aaccgttatt
     1021 attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat tgatggatgc
     1081 tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga tgacaccgtt
     1141 caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt cgtttggtat
     1201 ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg aaaaatctgt
     1261 tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc ctcaaggagc
     1321 tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc
     1381 gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc aaacggtgac
     1441 caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta ctcaggtcga
     1501 actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt
     1561 tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc agaaaacatc
     1621 agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca tcaaacaaat
     1681 caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg ggatgaccgt
     1741 gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag tcaatatcca
     1801 agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag cggaaaaagt
     1861 acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca atccgcaaaa
     1921 atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa
     1981 agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga agaagcgatc
     2041 cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata tatcgatgcc
     2101 atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc gattcctcat
     2161 ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca
     2221 gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt gttcggtatt
     2281 gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat
     2341 atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta
     2401 gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt
     2461 cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt cgtagatgtg
     2521 aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga attagcagat
     2581 gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca
     2641 gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat tggcccgaat
     2701 atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga aaaggaagga
     2761 agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc agcatttcta
     2821 gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata tatcggcttt
     2881 ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc tttattcgtc
     2941 caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc
     3001 caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg caaactattt
     3061 agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg ttaccagacc
     3121 attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa
     3181 acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca tgcgtatatt
     3241 gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct
     3301 cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt gagagaacta
     3361 caagaacata acttgacttg cccacatttg acggcaacaa tggggattat ttttaattac
     3421 tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg
     3481 gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg gaatatcaag
     3541 caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa aaggggaatg
     3601 tgacgaaatt ttt
//
LOCUS       DACCWV010000193         3607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCWV010000193 DACCWV010000000
VERSION     DACCWV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.198"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            58..510
                     /locus_tag="IVW84_002740"
     CDS             58..510
                     /locus_tag="IVW84_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918897.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="IVW84_002741"
     CDS             524..1213
                     /locus_tag="IVW84_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918898.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..>3607
                     /locus_tag="IVW84_002742"
     CDS             1338..>3607
                     /locus_tag="IVW84_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7918899.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLD"
BASE COUNT         1221 a          474 c          753 g         1159 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattt
//
LOCUS       DACCWV010000194         3589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCWV010000194 DACCWV010000000
VERSION     DACCWV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.199"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(19..1362)
                     /locus_tag="IVW84_002743"
     CDS             complement(19..1362)
                     /locus_tag="IVW84_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7918900.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(1496..1915)
                     /locus_tag="IVW84_002744"
                     /pseudo
     CDS             complement(1496..1915)
                     /locus_tag="IVW84_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2092..3233)
                     /locus_tag="IVW84_002745"
     CDS             complement(join(2092..3005,3005..3233))
                     /locus_tag="IVW84_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7918901.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            complement(3299..>3589)
                     /locus_tag="IVW84_002746"
     CDS             complement(3299..>3589)
                     /locus_tag="IVW84_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918902.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVT"
BASE COUNT         1002 a          681 c          566 g         1340 t
ORIGIN      
        1 gatttcattt agtaacgatt atgtttcaat gtttaatcct gttactgcct ttaaaataaa
       61 attatagggt tggctaaaac taggatgaaa taaataatct aacattttca atttgtctaa
      121 tgtaatttgt tcttctatag caagagaaaa cattgataat agtccagata aatcttcttg
      181 actacaaagt tgtacaccta ctattctctt tgtatttttt tcaaagctaa ttcttaattt
      241 tacacgacta ttttttctca aaaaaacagg tctaatgaaa tcttctattt ctatataatc
      301 tatagactca cttaataatc gtgctgtttt taaattcaaa cctgctgcgt aaaaattgta
      361 attaaatata gaaaagccat ttgtaaattg ggaaccagaa ggtgtaaaat cttgattgtc
      421 aatatgttga gttgcaatat aagctgttct tagagcatta gaaatagaaa aatcaacact
      481 acttctatta atggacgtaa aaaaagttgt agcacaatcc ccaatagcat atatatcttt
      541 tatatttgtt tgctggtatt cattagttag atatgctcca ctaacatctc tcaagagttc
      601 ctttttgcca attggactat tagatagaaa tcctatagct tgtacgacca tatccataga
      661 gtattgggta ccacttgtag tgactatttt ttcaatcttt tttcttccta aatatttttt
      721 aggactttct gatagacaaa gatgtattcc attttttgat agtatttctt caactttatc
      781 tgaaaaacta cggtcgtaat aagtcgacaa cactctatct gcaacatcga aaagatatac
      841 ttctttccct ctttttctta tggcttcagc aatttcaact ccaatatatc ctgctccaat
      901 aattcctact tttttaatag tattatcatc taaagcttta aaaatctctt tagcatttgg
      961 aaaattcttt atcgaaaaaa tattagctaa attattcccc ggtacatcta acataatttg
     1021 ctttgaacct gtagctagta taagtttgtc atattcttgg gaaaatcttg tgccatcttt
     1081 ttttacacaa ataatctttt tattttgaat atcaattttg tcaactgctg tctccaaaaa
     1141 caactttttt atcttagaat ttagttctct tctattttga taaaaaagat cttctaaatt
     1201 gctaacttct ctttttatga acaacgatgt cccacattct aagtaactaa tttctgattt
     1261 tttatctatc agaataactt cataatttga atcacataag tttagaaatc gagctgcagc
     1321 tagccccgaa tggtttgatc caataattac tatttttttc acttaatcac attcttttcc
     1381 cttctatgaa aagtatcttc atttttaact aacaacctat ctcccaaaat aaaaaacaaa
     1441 aagaggaaga aaaagattct tcctcttttc agagaagatt taagaaattg attttctatt
     1501 cattctcttt ttttcgattt ttatacccta ataaccctaa agttcctaac aggattaaac
     1561 caccactaat agtaaagata ttactatcaa tctctcccgt cttaggataa gcttttgagg
     1621 actgcttttg aggactgctt ttgcctctat ttttgttttg cttgctacct tgattgtcag
     1681 tcttattatc tttactaacg gctttttcac tgtctttttc gtttgaagaa acaactacat
     1741 tagtaggttc actagtatta ccagattcat cagttgcagt tactatcact tttgttcctg
     1801 cctctaattt aggaacttta actgtaaaat taccattttt atcagctgtc ccatggtatt
     1861 gactcccgtt tggtaatgta actgtcacct ctgcatttgg ttcagtttta ccagttggat
     1921 ttgagtcaat acctcggaca cgatttgtga gaattttttc gagattccag cgggtgagta
     1981 tctcatcgtt tcgaccagcg gtcaaaccaa aaccgtttga ccgctgatcg aagcgatgaa
     2041 gttgaaaccc tgtggaaatc tcgccaaaaa aatgtaagtg gtagctagga actaagcttg
     2101 caatgccaac tcctccatct gatttggtgt taaatagttg atactactat ggattcgctt
     2161 gcgattgtaa aatccttcaa tgtattgaaa taacgctaaa taagcagctt caaagttttc
     2221 atacgctttt gatggataga cttcttcttt tttgagtgag gcgtgaaacg attcgatacc
     2281 ggcattatcg tagggacagc ccttacgact gaaggagtgc cgaatgttca attcattcag
     2341 ctttgcctca taggcttcgc tagtgtactg agaacccaaa tctgagtgga tgatcaagtt
     2401 ctcggagggt gtttggttta agagcgcatt gtcaagggtt tcgataacca attctttcgt
     2461 catctgcttg ccaaacttcc aaccgatgac tttctttgtg tggaggtctt gaatagtgga
     2521 gaggtaggtc cagccatctt ttagggtatg aatataggta atatctgtca cccatttttg
     2581 attgatcgac gtagtggtaa aatcttggtt taacaggttt ttacgtcctt cacttttaac
     2641 tttcgcttgt ttgccaggct tgtacttctt gagggtgact gaacgcaagc ctaattttct
     2701 catcagtatt tgtactttct ttaaggacaa ggtctcgcca cggccaacaa gaatcttatg
     2761 aattttaggc gcaccataga tacccttgtt ttcaaagtag attcctgaaa tcaagcggtt
     2821 taagcgtttg ttttctgatt ggcgcttgga aggtttcttt ttcttccagt catagtagct
     2881 ggatttcggt gcttccaaaa cggtacacat ttgttttacg tcatgatcat gaacatttga
     2941 ttgaataaat tcgaagacat cactcatttt tacttttgag cgaatatggt taaagccttt
     3001 tttaggatag tgttttcctc ctgcatgcgg gccatttcct ttttcatcgc taggatttct
     3061 gcctcagtca tgccggttgc tacattcttt gtatgaagct tcttccaacg ataaatcgtt
     3121 tgtgtagcga taccatattg cgctgccagt tcttcgaccg attggccaga ctggtttaaa
     3181 tctaagatca tcttcttaaa gtcttcatca aattttttct tggcttttgc catcgtggac
     3241 acgtcctttt cttagtagtt agattctacc aaatcgtgtc catgaaatca tcctaacatc
     3301 aagtaacaat ttggtcaccc tctgtaatag gatcaacttg aggtttattc ggtgcttttt
     3361 tatcttctac agaaatagta acctctactt cttctttaga tccatctgga tatgttacta
     3421 ctacaatccc ttttttgttt ccagctgtac taacatctac tttctctttc caagtataag
     3481 tagttccttt tggtagatct tctttgttct taattccctc agatgcgtct ggctctttat
     3541 ttaagtctgt agtcactttt tgtccttcag gattgtattt atcggcatc
//
LOCUS       DACCWV010000195         3480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCWV010000195 DACCWV010000000
VERSION     DACCWV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.200"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1211..1537
                     /locus_tag="IVW84_002747"
     CDS             1211..1537
                     /locus_tag="IVW84_002747"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7918903.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1545..2453
                     /locus_tag="IVW84_002748"
     CDS             1545..2453
                     /locus_tag="IVW84_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7918904.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3082..3333
                     /locus_tag="IVW84_002749"
     CDS             3082..3333
                     /locus_tag="IVW84_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP7918905.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         1092 a          690 c          599 g         1099 t
ORIGIN      
        1 tttacaacga tttatagaaa aacaacctct ttaaatataa aaggtacggg ttactgatta
       61 cttgtaaatc tttacgttcc agttttattc tttctaaaaa atatactcac ttcttttctt
      121 agacttcttt ttttgattct cctctttatg gtcttgtcct tttttgcctt caaaatattt
      181 aaataaaccc ctttcaaaag catttctagg ttttggacaa taaggaatga cttttttctt
      241 catataaata cctcatttaa ctatttgata tcatcacatt atcaaaaaaa ataggttttt
      301 tattaaaact atcttgacct ttcgggagat ttcactgtga tttattttta ttcccatact
      361 tttttcgttg gtcactttcg tatacctaat ctaagcgatc atcggcgacg gtgcttttac
      421 aacgaatggc aggacaaaac aaggggcgga aaaacttttt tgcctatttc ataaaaaact
      481 ttttcctaca gcaatccctt gttttgtcct accacacgtt gtgaacccaa agcacgccga
      541 cgaacgctta gctatacgaa aatgacctcc aaaaacaaaa aagtgaggtg aatagaaaca
      601 aaaagaaatt gaaatctccc aaaatgtcgt ccgcctcgga gacaacttgc tgctgaaaat
      661 ggttggttaa ttcatttaag tattaaataa gataaatcat caaattaatc ggaaaaatag
      721 catgattggc cttatcaaaa ataaactgta aaaacttata tttctatgag tggtggaaca
      781 atgctaatac caaattaatt tatcgattac ccacctgcac aaaccaaaga tagcattatt
      841 aggaaacgaa tcaatcagcg aatccagcat tataatgagg atactaaaaa taaagttttc
      901 ataattataa atccgccttc ctacgaaagg caccaatgag gtgatgaaaa ccttagagcg
      961 ctttctttct ttttttattc cgtgttaccc tttccttgca ggtccctggt acactacaga
     1021 acccgaggag gtgagtggcg gggctgtata gcggcaaccc cgcataatta tatggtgtcg
     1081 gtcatccgtt aaggcatcaa tgaatggcgc aaacccgtag cccgtaaact tatctcttcc
     1141 gaagttgact cgatttcgcc ggatgaccag tccctaccaa acctgtacta tttttttaca
     1201 ggaggttgct atgttcaaaa tctttcgtaa aaattgttgt ggcttggatg tccacaaaac
     1261 gtggatctat gcgtgtatcg gaatcacaga tgcgaatggt cgtacagtgt ataagcaagc
     1321 tcgcttctct tcctttacaa aaggtctcaa tgaattagct gaatggcttg ctaaatacaa
     1381 ctgtaaggat gtgtgtttgg aatcttccgg taaattttgg attcctgttt ttaatattct
     1441 tgaaaaaact tgttttgtca cactggctca tccgaagtac accaaacccc agaaaggaaa
     1501 taaaacagat cgcaaagatg caaaaatgga tttgtgatct ctttatgtgc gacatgatca
     1561 aacccagttt tattcctggt cttgaaatcc gccagcttcg tgatctaatg cgttatcgga
     1621 cgaaactgac caacatgaaa acaagtgaaa agaatcgtgc tttaaactgc ttaaccgttt
     1681 ctaatctgaa actagatgat gtttttagtg atgtgttcgg aaaatcatcc acttctatca
     1741 tccaatactt gttagaacat cccggtgaat catttgatgt tcgaccattt atccatgccc
     1801 gttgtaaaac tcccgttgaa gaaatccaag cggcagttga gggggcgatt tctgtacctc
     1861 aagcgattaa gcttcgccaa tgcctcaacc atattaacga gattgagaag catcgagaag
     1921 agatcaatct agagattcaa gctcttgcag aacccttctc ccctgttttg gaactacttc
     1981 gtaccatacc gggcttagac actcagccaa taacagcgat tgcgattctt tcagaaatag
     2041 gaccggatat gtccgtgttt ccaacttcga aacactttat ttcttgggct ggatgctgtc
     2101 caagaaacga tcgcagtgcc cgacgaacaa aatcaacacg tatctctcgt gcagggcagt
     2161 cgttgaaacc gctacttgta cagattgcca atgctcttct cagatccaaa gaacatccag
     2221 aatttaaaaa tcgctatcgt agaatcaaag cgaatcgtgg acacaagaaa gctatcattg
     2281 ctatttgtaa gatgctcctg accgctatct ggaatgtctt atcaaaggtg gaaccctatt
     2341 ctgctaaagg atatctggag ccaaaacctg cgaaagaaga gaaagttctg acgcaatccc
     2401 aagctctgga ccttttaaga aaacgcggat ataccattat agatagtgct taaaccaaag
     2461 ttacgttgat tcttcctaaa tagactcgtt gtcttccatt tcaaaaattt tgaaaacggt
     2521 aggcttattt agtgtaccgt tcttcaggta tcttcaaata gatttatttt caaacttatt
     2581 tcctttcttc ttttactctc ctatagctta gcatttaagt gggcactcgt gtcaccttgc
     2641 tagatagcta tgaatgccgt aataagcctc ctagtatata ggcagactgc caattataac
     2701 tgattcaatc cacattatga taaaatccac cttttcgttt catatgtgtt gaaatatcac
     2761 atatgtcaaa agtgcacgag tcttttcatt cagtgctttg taaatactca aaattattcc
     2821 ttttcacttt aaaaaaaaga taattagtac aaaatcagaa atgaggaaag tattaggatg
     2881 gactagaaag gagttgttga atttgttgca acagattggt catttatcaa ttaaaaatta
     2941 ggtgaagttt tattcatcac tgcagctgct gtttcccaga gaataaaacg tctagaagaa
     3001 actggtcagt tactgtatta tcaagcagca attgactacg aacaagcttg attactaacc
     3061 aaagcgttta tccacctgtt gattaaatca aatcaaaaat caggttttta tacgttcata
     3121 aaagcaatct ccagtgtact cgaatgtgac tgtatcatag gtccttattc gatgttaatc
     3181 aaaatggggt ttccttctac cgaatcattg gacagtttta tcaatgattt atcaaagttt
     3241 ggacgaacca acacccaaat cgttttctcg actcttgttt ccttaatggt ttatcagttc
     3301 tggaatgttt atcatgcgct tttattaaaa tagaaaagag cttttttcat gcgacaaagc
     3361 agtgatcatt tattggcaca tgcaatcttt gtggatccat tttttgaaaa tacgatattt
     3421 agttttgacc caaaaatctt taaatacgta tttgataatt tatcttaaca accagataat
//
LOCUS       DACCWV010000196         3479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCWV010000196 DACCWV010000000
VERSION     DACCWV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.201"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            28..909
                     /locus_tag="IVW84_002750"
     CDS             28..909
                     /locus_tag="IVW84_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7918906.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="IVW84_002751"
     CDS             922..2532
                     /locus_tag="IVW84_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7918907.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="IVW84_002752"
     CDS             complement(2609..3268)
                     /locus_tag="IVW84_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7918908.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT          953 a          677 c          697 g         1152 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaa
//
LOCUS       DACCWV010000197         3478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCWV010000197 DACCWV010000000
VERSION     DACCWV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.202"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            148..948
                     /locus_tag="IVW84_002753"
     CDS             148..948
                     /locus_tag="IVW84_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP7918909.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            964..1842
                     /locus_tag="IVW84_002754"
     CDS             964..1842
                     /locus_tag="IVW84_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7918910.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1855..2835
                     /locus_tag="IVW84_002755"
     CDS             1855..2835
                     /locus_tag="IVW84_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7918911.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT         1197 a          563 c          720 g          998 t
ORIGIN      
        1 tcatattata atgtttcttt ttaataaaaa gattgttttt aagctaatta ttatatgtgt
       61 gttattgaac taactcttga cagataatca aaaagaattt atagttgtta ccgtagtagc
      121 tactaaaaat aaagtgagga ttaatcaatg gaaacgatta ttcaaatcat gaaaaaatat
      181 gatttttcag agatggagac aagagtgtat accacactat tagaaaaagg taatcttaca
      241 ggatatgaag taagtaaaat ctcaggtgtg ccacgctcga aagtatataa tattttggaa
      301 aaactattaa aaaagaattt gattgtcgtt aataaatcag aacctaaact ttatcatgcc
      361 atctcggcaa atgagttttt agagaaatta gaaaaaagtg ttaaaaatga tctttctttt
      421 cttaccaaga atctagggat gatcaaagaa aaagatgaag aagaaatgct gtggaaagtt
      481 gatggtatcg agtatgttct ggataaagta gagcatttgg tgaaaaacgc aaaagaaagt
      541 ttgctcattc aagtttggca tgagaatttc acggattcac tactaaaggc attgcagcaa
      601 gctgaaaaaa gagttgataa gtttgtactg attttattca gttctaccca tgaatatgat
      661 cttcccttag aaaagtatta tattcatggt tttgaaacag acaaacttgc tgactttgga
      721 gcaagatgga tcaacatcgt cgccgatgag caagaagtcg tgtttggtac cattaacgaa
      781 gaactgcaat caaccgatgt cacttggaca aaaaaccacg ccatggtcaa tctagcaaaa
      841 gaatatgtca aacatgatgc atatacacta aaagtgattg cagaatctcc tgaagaactt
      901 aaagcaaaat atggtgaggg ctttgaggga atcagaaaga tttactagta ggaggaattt
      961 tttatgattc ggaatatttt gctgacacta attgtcgcaa tcaatctata ttttattatt
     1021 tactttctaa gagacctttt caaaaacaaa caaaatttca aagaagaacc aggggatatg
     1081 cgtctgttgc ccttcacgtc atttatcatc ttctttcttt caacatttgg cgtttccgac
     1141 tttgctatcg gaaccgtcct ttatccaaag ttgaaatggg tcagcatgaa aaaactaccc
     1201 ggaacgttga atacccaatg tgtagtaccg gtagccgtca tggcattgtc ttacatcact
     1261 gccatcaacg tagggatcaa gacactagct gtttgtatta tctgccaaat tatcggtgca
     1321 tatttaggac caagatttgt cgtgaagtta ccagagaaaa cgatcaaact gtttgtcggc
     1381 ataggattgg tcattgctgc attgttgatt tttgcaggac aaatgaactg gattccatca
     1441 aacggaacag ccagtgaatt gtatggcgga aaattaatct tggcaggatt tttactcttt
     1501 gtctatggtg cattgaacaa tatcggcatt ggttcttatg cgttaacgat ggtaactgtc
     1561 tacttgctag gcttgaatcc agttgcagcc tttcctatta tgatgggggc ttgtaccttc
     1621 tcagtaccga tcggcagtgt tcaattcatt aaatttaatg aatacagcag aaaaatcaca
     1681 ctttttactt caaccttcgg tgtattgggg gtattggtcg ctgtattcct agtcaaatct
     1741 ttagatacct atatcctgaa atggatcgtg attttggtct tactatacag tgcatatacg
     1801 atgttgggca gtcaattaaa aaaagttaca gcagctaatt aaggagcgat tcaaatgtta
     1861 tttcttaaaa aagaagatat tatcaaaagt ttttcaatga gagaagccat cgatgcggac
     1921 aaaaaagcac tgtcattgta ttcagcagga aaggcaagtg tgcctcttcg caccaatatc
     1981 gatgtgccaa aaagcaacgg acaaagctta tatatgcctg cttatgtaga aggtggcgaa
     2041 ggcgcgctag gtgtcaaaat cgtttccgtg tatcctgaaa acatcaagaa aaacttacca
     2101 agtgtaccag caacgatgat cgtattggat ccagaaacgg gtatggtatc cgcttgtttg
     2161 gatggtactt atttgacaca attaagaaca ggcgctgttc aaggagcagc aacagaactt
     2221 cttgcaagag aagatgctaa aattggcgcg ctgattggaa ccggcggaca agcacagtca
     2281 caattagaag caatgttgac cgttcgtaaa ttagaagaag ttcggatttt tgatattgat
     2341 tttgagcgtg ccagccaatt tgctgaaaaa atgatgcaac aattttctgt aacgatgcgc
     2401 ccaacaaaaa ccagtcaaga atgcgttgaa ggagcagaca tcattacaag tgtgaccact
     2461 tcaaaacgtg cgacattctc tgctgagtgg gtcaaaaaag gtgcgcacat caatggagtc
     2521 ggtgcttaca caccggaaat gtgtgaaatt ccacgagaaa taatcaaagc cgcagatgtt
     2581 gttatttttg ataccatgga cggtgttcta aaagaagctg gggacttcat ttcaccgctg
     2641 caagatgggt atattcaaag agacagctat catggggaac taggccaact gatcaatgaa
     2701 gaattgatcg gtcggacaag tggagaacaa atcacgatct tcaaaacagt tggttcggct
     2761 gtattagatg tggtagttgc gacagaaatc gttaagaaag ctaaagaaaa taatttggga
     2821 aaattgattt tctaattaaa aaggctgaaa ttaaaacctt tattattgcg tttaaaaaaa
     2881 gaaaacaaag aagtttgtaa tcaaatggaa actgacccca taatttaaat aaaataaaaa
     2941 acatctcttg ttgaattcta gtaatgaagc gaccctaaaa cttagaccaa aaaatctaac
     3001 ttttgggtgg agttgtcaag aaaaaccggg cacttagtta aaaaataagt tggacgggtt
     3061 atactaaaat tgacaccaaa ttaagagaaa gcgaagatct ggattatgcc aagacgttca
     3121 tttgatgaaa tatttaaaat taccgctgta aaactggtaa cggaagaagg gttttctgtt
     3181 aaagaagtta gtctatagct agaagctcgc tctaatagcg tgtattgatg agttcaagaa
     3241 gttgaaaaat atggataacg tacttttctc ggtagaggaa gtcatgaaat ccattcgcag
     3301 aaatgaaatt acacgcttag aaaaagagaa taaacggtta aaagaatagg tagaactcct
     3361 aaaaattacc gaagtcttcc tgaaagaaaa ggctcttcgt caaattgtgt tggcgaagac
     3421 aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat atttttta
//
LOCUS       DACCWV010000198         3414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCWV010000198 DACCWV010000000
VERSION     DACCWV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.203"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2232)
                     /locus_tag="IVW84_002756"
     CDS             complement(<1..2232)
                     /locus_tag="IVW84_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918912.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNSGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IVW84_002757"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IVW84_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          946 a          757 c          545 g         1166 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctga
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttt
//
LOCUS       DACCWV010000199         3327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCWV010000199 DACCWV010000000
VERSION     DACCWV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.204"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..272)
                     /locus_tag="IVW84_002758"
     CDS             complement(<1..272)
                     /locus_tag="IVW84_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7918913.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="IVW84_002759"
     CDS             complement(244..1443)
                     /locus_tag="IVW84_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7918914.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..2949)
                     /locus_tag="IVW84_002760"
     CDS             complement(1573..2949)
                     /locus_tag="IVW84_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7918915.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2949..>3327)
                     /locus_tag="IVW84_002761"
     CDS             complement(2949..>3327)
                     /locus_tag="IVW84_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7918916.1"
                     /translation="YLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQR
                     VAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADS
                     ADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         1022 a          635 c          479 g         1191 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacgctcct ttttttgtga cttttctgaa gctggagtgg acactttcaa ccgcattggt
      421 agtgtacata atttttcgaa tggcactacc ataatcaaat agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2881 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3301 ctaatatttt caattggtga taaataa
//
LOCUS       DACCWV010000200         3309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCWV010000200 DACCWV010000000
VERSION     DACCWV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.205"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..707
                     /locus_tag="IVW84_002762"
     CDS             <1..707
                     /locus_tag="IVW84_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7918917.1"
                     /translation="DIKKNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHP
                     TSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLE
                     NMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKI
                     IAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSV
                     ELREKVLRSIIDKSIE"
     gene            937..1167
                     /locus_tag="IVW84_002763"
     CDS             937..1167
                     /locus_tag="IVW84_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7918918.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1390..1488)
                     /locus_tag="IVW84_002764"
                     /pseudo
     CDS             complement(1390..1488)
                     /locus_tag="IVW84_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1753..2208)
                     /gene="tnpA"
                     /locus_tag="IVW84_002765"
     CDS             complement(1753..2208)
                     /gene="tnpA"
                     /locus_tag="IVW84_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7918919.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2451..3309
                     /locus_tag="IVW84_002766"
                     /pseudo
     CDS             2451..3309
                     /locus_tag="IVW84_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1091 a          576 c          578 g         1064 t
ORIGIN      
        1 gtgatattaa gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa
       61 ttggtttttc tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa
      121 aaaaagaaca tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag
      181 ctctctattc cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga
      241 tcactcaatc aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg
      301 atttagaaaa tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt
      361 ttcaagccca agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat
      421 ctgggaaaac gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa
      481 ttattgctat aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac
      541 aaacagccat tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt
      601 atatttgtga tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat
      661 taagagaaaa agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt
      721 agctaaacct aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt
      781 gatagcttat tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct
      841 acggttcttg attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag
      901 aaaaacgtgt agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct
      961 ctcttaaata aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag
     1021 gaacctactg aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg
     1081 ttttctataa agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt
     1141 gtttataaga acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact
     1201 tcaatgttat gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta
     1261 aaagtttatg atccttttga tttttatatt aatttatctg tatttaatac agattttatt
     1321 tattagttca tctgcctatt gtattttatt ttgttgcctt accatctgaa gtaaatatac
     1381 tcttaattgt caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc
     1441 caaaggtgtt cgataatcca gtgattttct aggaagatta tttctgcgcc agcattttct
     1501 aatgtttgaa tttgtgtttt gtaatcttgg ttgcttgtgc taattcttga atagccgtaa
     1561 attgccaaaa tttcatcacc ctttctggac ataagtttct gacaccagtt aaaatcaagt
     1621 ctatcagtaa cccatacacc cgtgaggacg ggtgtatttt taatccccct agaaggggtc
     1681 gacactcgct gccccttaaa aggggatttt taaaagaccg ccaactgctc atcttcactc
     1741 acttacttct ctttattttt taaatggttc tacgtactct cttttactta taatatatcg
     1801 aatgcgatca tcagcttctt gttcacggat gtatttttca tttgtctttt gattcaatcc
     1861 gactgtactc acatagtatc tcttcgacca aaaggatttg tttccgtatt tatatttgag
     1921 atttgcgtgc tgctcgtgga ttagtaccgc actacgacct ttcagatacc ccataaaact
     1981 ggatatacat atttttggtg gtactcgaac aagcatgtga atatgatcta gcattgcatg
     2041 tgcttctatg atttccacat ctttcatttc gcacaacttc ctaagtattt ttccaagatc
     2101 ttttcgtaat tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa taatatgata
     2161 cttacaattc caacgagtat atgctaaact tttatcgtca tttgacatga aaaaactcct
     2221 ttcgatctga tgagcggttg gtagccactc actagtatat cgaaaggagt ttttatcgct
     2281 aaagatactt cttccacgcg tagaaacgcg cggttttctt gtttcgcacg ctttacgcac
     2341 aaaactgact gaagtcatta agaaaataaa agggtctaaa acttttaagt tttgtaccga
     2401 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggctaagt
     2461 atacttttga acttaaatta aagatcgttc atgattactt ggatggtaaa ggcggaagtg
     2521 attatctggc aaagaaatat tccattaagg ctccatcaca agtgaagcgc tggatcaatg
     2581 cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa aaatattctg
     2641 ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc tatcgtgaag
     2701 ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg cgtcaattcc
     2761 gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga
     2821 aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac
     2881 gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag
     2941 aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa tcgcacgaat
     3001 catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaactt taaagttccc
     3061 taaggcaatc tttatgtact ggcaaaaacg attggaccgt aaaaatccag atcaagagat
     3121 tgaagcggaa atgcttaaag ttcgggagaa gcacaaggat tatggctgtt tacggatgac
     3181 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcctgatcaa
     3241 gaagctagga ataaaagtaa cgacatacac acgtaaatca cggcgctaca attcttatcg
     3301 aggacaagt
//
LOCUS       DACCWV010000201         3234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCWV010000201 DACCWV010000000
VERSION     DACCWV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.206"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(552..2165)
                     /locus_tag="IVW84_002767"
     CDS             complement(552..2165)
                     /locus_tag="IVW84_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7918920.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="IVW84_002768"
     CDS             complement(2306..3214)
                     /locus_tag="IVW84_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918921.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1040 a          556 c          571 g         1067 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atat
//
LOCUS       DACCWV010000202         3213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCWV010000202 DACCWV010000000
VERSION     DACCWV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.207"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            152..841
                     /locus_tag="IVW84_002769"
     CDS             152..841
                     /locus_tag="IVW84_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7918922.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            855..2357
                     /locus_tag="IVW84_002770"
     CDS             855..2357
                     /locus_tag="IVW84_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7918923.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2370..2846
                     /locus_tag="IVW84_002771"
     CDS             2370..2846
                     /locus_tag="IVW84_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7918924.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2943..3053)
                     /locus_tag="IVW84_002772"
                     /pseudo
     CDS             complement(2943..3053)
                     /locus_tag="IVW84_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1063 a          435 c          617 g         1098 t
ORIGIN      
        1 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
       61 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttattatcca
      121 aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt tagatcttat
      181 ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt acaaagagga
      241 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
      301 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
      361 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
      421 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
      481 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
      541 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
      601 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
      661 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
      721 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
      781 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
      841 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
      901 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
      961 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     1021 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     1081 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     1141 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     1201 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     1261 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     1321 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     1381 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     1441 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     1501 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     1561 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     1621 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     1681 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     1741 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     1801 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     1861 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     1921 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     1981 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     2041 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     2101 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     2161 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     2221 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     2281 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     2341 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     2401 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     2461 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     2521 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     2581 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     2641 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     2701 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     2761 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     2821 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     2881 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     2941 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     3001 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     3061 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     3121 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     3181 tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACCWV010000203         3041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCWV010000203 DACCWV010000000
VERSION     DACCWV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.208"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..233)
                     /locus_tag="IVW84_002773"
                     /pseudo
     CDS             complement(1..233)
                     /locus_tag="IVW84_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
     gene            927..1313
                     /locus_tag="IVW84_002774"
     CDS             927..1313
                     /locus_tag="IVW84_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7918925.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1411..1602
                     /locus_tag="IVW84_002775"
     CDS             1411..1602
                     /locus_tag="IVW84_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7918926.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2116..2448
                     /locus_tag="IVW84_002776"
     CDS             2116..2448
                     /locus_tag="IVW84_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7918927.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2358..2561
                     /locus_tag="IVW84_002777"
     CDS             2358..2561
                     /locus_tag="IVW84_002777"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7918928.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2745..2900
                     /locus_tag="IVW84_002778"
     CDS             2745..2900
                     /locus_tag="IVW84_002778"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7918929.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         1119 a          495 c          464 g          963 t
ORIGIN      
        1 tctggtttta aacatgtata gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa
       61 atctttagtt tttttaatat tatcagttag taacttttta taatcataaa aaaataagta
      121 gttctatctg agcgatactc ttttataata ctgaattcgg aattaaaaac cacgataggt
      181 aactttaaac aaacatgaag agactttaat aagacttctt tatctgaaaa cacttttgtt
      241 tctctctttc ctttctacat cattaaatag tattttatac gttatctaaa atactattcc
      301 tctgtcaaaa taagctaact tacttcatta taaaatatcc acttatcgct ttcatgtata
      361 ttccaccgta aaaagtacat tttatcaccg agaactcatc atttttcatc atgatgaata
      421 ctatttacat atatctagtt caaagacaat aattattatg atccgttatt atctaagcac
      481 tatacagata ataggaattt ttcgtttcta aatactccaa tttgggaatc caataaaaat
      541 tttcgttcaa atacgtgtct attcgaactc ttttctagtt gttaattatg aagggggaag
      601 aaataaaaaa tacaatctat acacgaataa gaatattatc caaaaggagg ttttagaaaa
      661 gatttattat acgtaaaaag ctcaagacat tttcttagcc ataaggacat gtttgatatt
      721 taagaagata tatgaatttt cttttctaag tataaagata actatccatt atcaactaaa
      781 aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct tatagaagtt
      841 attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc ttttcccttt
      901 aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta aatgcccagt
      961 tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat aaaaaacaag
     1021 aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt actaaagata
     1081 ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt gaagatataa
     1141 aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt atcgaaaaat
     1201 ttatctttaa taaaactgat aaagaaattg gaaaggttct gggtattaca agacagggtg
     1261 cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt taatatctgc
     1321 ataataacta attttgtatg cttctttgct cgtcctcctc cttccccaaa aaataaatag
     1381 attataagtt catgggaaga aggtaaattt atgaatactt taaaagaaga tttcatttta
     1441 gcaaagaatg gaaatgaaga agcagttgtg gccattctca aaagatttag cgctttggtc
     1501 cataagcaat cctggagaac tggaaaatat gatcaagatt gttatcaaga atgtatgctt
     1561 gcaatatatc tagctatctc gaaattcgaa attaaagaat aactcaacta agggagtctg
     1621 tgacataagt aaaaagatag tttcaaattt cgaacaataa atttgaagcc atcttcttta
     1681 taattataaa gtatcgattt tacagcgttc tataatgatt catagaatct aaaacgaatg
     1741 attttaatta ttaacgtttt ttaattcgtt tttagcttaa aaataacttt tgtcacagtc
     1801 tcactcctaa aattacttta tttacgtacg aagtgggcag gaggtcgaat ccagaactgg
     1861 gccatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga tttaccttcc
     1921 gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga tttcaaaaat
     1981 agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag tttagttgac
     2041 aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt acgttaccgg
     2101 aaggaatgaa ctctattgaa aaaaacatat gatcgtaaat ttaaactaaa gatttcacag
     2161 gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa tatctctcgt
     2221 ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga
     2281 caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga aaataaacga
     2341 ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct ccagggatca
     2401 ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc
     2461 gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat ggtagagaaa
     2521 aaacaatcaa agaaatggta aaaaaaatgt tgaatcgata atttgaacag aaccaaattg
     2581 atgctgtttg ggtgacggat attacttaca ttccatgtaa tgacggtcgc ctctatttgt
     2641 ccacctatat tgacttagca actcgtattc tacgttacta tatggtggat tcacacatga
     2701 aaaaagagat tgtcgtccgt ccattacaaa tttattaagg agaattgcct acggtaattc
     2761 attccgatag aggtagccag catcggtctt ttatttatca agagctgttg accgaccacc
     2821 acatcacaca ttcaatgagc caaactggga ctcctgttga caatgcagta attgaagcgt
     2881 ttcatcgatc aatcaaatga gaattaatcg actccaacaa atataaaagt aaaatagaga
     2941 tgaaggtgtt aatccaagat tatttagaag actactaccc aaacaaaaga atccatacaa
     3001 aatttatgat gactccttga caatttgaag cgaagaaaat a
//
LOCUS       DACCWV010000204         3040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCWV010000204 DACCWV010000000
VERSION     DACCWV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.209"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="IVW84_002779"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="IVW84_002779"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7918930.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="IVW84_002780"
     CDS             complement(1036..1956)
                     /locus_tag="IVW84_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7918931.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="IVW84_002781"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="IVW84_002781"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7918932.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1046 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat
//
LOCUS       DACCWV010000205         3015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCWV010000205 DACCWV010000000
VERSION     DACCWV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.210"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(193..1503)
                     /locus_tag="IVW84_002782"
     CDS             complement(193..1503)
                     /locus_tag="IVW84_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7918933.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1515..2933)
                     /locus_tag="IVW84_002783"
     CDS             complement(1515..2933)
                     /locus_tag="IVW84_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7918934.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          940 a          624 c          471 g          980 t
ORIGIN      
        1 cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt atcacgtcta
       61 aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa gcgcgacaac
      121 gttgtttaat atatcattaa aaaaaataaa ggtcaagcta aaatattatt taagcttgac
      181 cttattataa tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc cagtttctat
      241 agataaatca cagcttttga aacataagtt ttgtactgct tcgattcgcc cgaattcttt
      301 tgcagttatt tggcaatgag aaaagcagac atcttctata ggcttttcag gaaatgctac
      361 taaatcaaaa gctcgggaag gaagtgagta acctggagaa aggcatgcct gtacgttttt
      421 gatgtgtatg tctttgactt tcgtcatttg ctttgattca ggtatttctt cagccactgc
      481 ttgccaactt tctggcattt gagcaaggtc attgctattt tttctattgt attcattatg
      541 ccagtccatt atccaagaaa aaggaaattg tacatcaatc atattcagat tttctaccct
      601 gatattttcg atcactcccc ctctttcttt tgaagatttc attcgaatac cacagtctga
      661 ctgaaaaaaa tcgatatcgt gaatatatac gtcactaact ccagcactaa cttcgctacc
      721 aatagttaca ccgtatcctg aacggatttt acatctagca acctcgattc ttgatgattt
      781 tcgattgact cttgcaccat tgccatctct gccagactta atagcaatac aatcatcacc
      841 acaggccaaa tcacattcat agacacgcac atttgttgaa gaatcaatat caatcccatc
      901 tgtacttggt ccatcattgt tgcggatgat tactttttct actaatacat cgttagaata
      961 agtgatttgc aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa caacaacatg
     1021 ttctgcatga tacagtaaac aagcgcgtgg acgtttaata gcataatccg caatccaacg
     1081 aagtcccttt tgatcgtatt cagctcttgt cccgcttttt tgatcttttc cccaatacag
     1141 ctcccaccaa tggtctcctt gaccgtcgat tattcctttt ccttctataa gaatatcttt
     1201 cgccgagaga acattcaaaa tcgctgctgg ccatttcatt tctacgccgg ctactcgtgt
     1261 atcgatttct ggaaatgctt cgatatctgt tctccccttg aggactgcac cttcttcaaa
     1321 gataagatga ctcttatctt ttaaaaaaag ggctccgact aaataaatgc cttttggaat
     1381 aaatacaggt aagtgttctt tcgcaccttg atcgattgct ttttgcagta ttattgtatc
     1441 atcttgttga gtgtcgtttg ttttaaagtt caaagcattg ataatccctt tttttgtccg
     1501 caatacgttt ctccctatct ttcattgatt tttttcaatt cttcaccaat aaatacagtt
     1561 tttgaacgat tcagtgggta acctactagc aaaatgattg cggatacaaa taagcagaat
     1621 gctggtaaca agtttgccat cgtataaatc ctttcttgta ctaaaactga ttggatggct
     1681 ccacctgttg tagacgattg gtaaccaatg agtgccagca taaatccgcc taccgctcct
     1741 gcacatgctt gtcctacttt tctcgcaaaa gaatttactc catatactgt tccatcttct
     1801 cttaagcctg tcttatattg atgataatca atcacgtcag taatgaatgc ccagaccatc
     1861 aaattgaata aaccagatcc taaagtagcg ataaataaaa gaatcagata tacccatgca
     1921 tttgttatat gaaggaaata catcagcaag tacatacatg aagaagcaag taacgcaaat
     1981 acgcttgctt cctttttacc aaataatttt gtcagctttg tagcaaatgg tgctaaaact
     2041 actactgttc cgtaagtaaa taaaagagca acagacattg cttgtttatt atggaagtaa
     2101 tcattgaata aataagtcat attcgtccca gctaaaatct gattgatcac aatgaaaatg
     2161 tcaacaatga ctaaaataat cagtgctcga ttagctaaca aaccttttac caactgagaa
     2221 gcagatactt tttcgttttt gacaactctt actctttcag tagtcaattt acaagttaac
     2281 atatatgcga caaatgctat tgcagcacat ccgattgaaa tccaaaagaa atgattccct
     2341 gaaacgactt gttgtccact cttgttatgg gtatacatta aaatcggaat gaagaatcct
     2401 gtagttgatc cgccaatcgc agaaccaatg cttcggaacg tggacaacga ggtgcggtca
     2461 ttaggatttg agctaattgc tgctgccatt gctccatacg ggatattgat tgttgagaga
     2521 aaaatcccat aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt cattggaaga
     2581 tgttggacga atggcaaaaa caaaatgaca gtcactaagc aaaatggata cttcattttt
     2641 ctgatccaag gaataaatcg gccagtttct gttaactctg agacatcaca taatcttcca
     2701 accatcacat cagcaaacgc atccaaaatt ctcgaaatca agaataacgt acctacgatt
     2761 gcccctgaaa cgccaagaac attcgtataa taaatcatca agaaactatt gactaaacct
     2821 aaaataaaac agttacctag atcaccgaac atgtagccta tcttatcgga aatcccaaat
     2881 tccttttttt ctttttttat tgtatttgct gtaacagcat tttgtgaaag catttgaatt
     2941 cctcctacct cttctagtaa acgttttcaa aattataact ctttttttct tgtttttata
     3001 ttcacaatgt tggct
//
LOCUS       DACCWV010000206         2984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCWV010000206 DACCWV010000000
VERSION     DACCWV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.211"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1768
                     /locus_tag="IVW84_002784"
     CDS             <1..1768
                     /locus_tag="IVW84_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7918935.1"
                     /translation="YPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAW
                     GRERTIKSRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNF
                     LIQAEVDTWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLF
                     RKRLARGKWREKDRPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGER
                     ENDCAGLGDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWI
                     ISTPNRKPNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDS
                     HLSADQQGEVFHRYVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWAS
                     DDSDAIERLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYEL
                     DLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAI
                     VAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQIND
                     QNRRPETFDFDSKIWVITQEEK"
     gene            1771..2946
                     /locus_tag="IVW84_002785"
     CDS             1771..2946
                     /locus_tag="IVW84_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7918936.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1010 a          438 c          565 g          971 t
ORIGIN      
        1 ttatcctgtt attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat
       61 cgagaatatt agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt
      121 atctggtgct tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca
      181 atcaatcgaa tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa
      241 aagaaaaaat actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg
      301 aaacttctta attcaagcag aagtagatac atgggatgtt actagactac aagttggcat
      361 caatccattt ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc
      421 gattctggtt tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt
      481 tagaaaacgc ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa
      541 ttgggaagca acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc
      601 atttgatgtt ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa
      661 cgattgtgca ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg
      721 cagcctttca gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga
      781 aatggtgaat aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc
      841 taatcgaaaa cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt
      901 tgttgaaaat attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat
      961 taagtgggat atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca
     1021 acaaggagaa gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat
     1081 aacaaaatat cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc
     1141 tggaatgctt cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag
     1201 gttaaaaatt caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt
     1261 atcaattgtt ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt
     1321 agcttttttt ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt
     1381 gaggattgta aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg
     1441 tactttttat agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt
     1501 tagtcatgat aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc
     1561 tccttaccgc cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca
     1621 aaatagtttg cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc
     1681 tagtggacaa ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat
     1741 ttgggttata acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa
     1801 ggagcaatct taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt
     1861 ccacaaatga aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca
     1921 ccaagtataa ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga
     1981 atgaaaaaga caataatttt aggtttatta ttagctggta taggaggaat tattccagta
     2041 tttacagcta ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga
     2101 gtatataatt cattggctgt gacatatatc agctctttat actctggcaa tactagagca
     2161 acgctattag gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt
     2221 gctggagttt tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt
     2281 cctgtagctc tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata
     2341 cctgaaaatc atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca
     2401 atcttactcg taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa
     2461 attaaagggg taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata
     2521 ctagcaggat ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg
     2581 ggaattatat tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg
     2641 ttcctgctaa cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc
     2701 tttagtaact tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt
     2761 tttattggct gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt
     2821 ctaacaagaa gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg
     2881 atcctatttg taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct
     2941 agttaatttt aatatcctaa agaatatctc aaagttcctt caaa
//
LOCUS       DACCWV010000207         2941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCWV010000207 DACCWV010000000
VERSION     DACCWV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.212"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(22..564)
                     /locus_tag="IVW84_002786"
     CDS             complement(22..564)
                     /locus_tag="IVW84_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7918937.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            699..1088
                     /locus_tag="IVW84_002787"
     CDS             699..1088
                     /locus_tag="IVW84_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918938.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1092..1856
                     /locus_tag="IVW84_002788"
     CDS             1092..1856
                     /locus_tag="IVW84_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7918939.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1985..2932
                     /locus_tag="IVW84_002789"
     CDS             1985..2932
                     /locus_tag="IVW84_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7918940.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
BASE COUNT          935 a          498 c          612 g          896 t
ORIGIN      
        1 gacagttttt aagtttgtga gttattttcc ttgtcgtttt ctcacttttt tagtccagaa
       61 accacctgaa atatattttg gctcataaga aatgataaaa gcacgctctt cgatttcttt
      121 gatcaggcga tacagttcac gttcgctttt tctaggagaa agtatttcga gaactagccg
      181 ctcaccttcc agccccattg cagaagtctg tgtcacacca taaccgtgtt ctctcagtat
      241 acccggcaaa cttttatctt ctgcgctatt tgctggcaaa atcgttgtga ccatgatgta
      301 tcctaatgcc aatttgtctt caatcaaaat tccgacactg atacctactg cgtaacctaa
      361 cgcatagaca aataaattca gtggattatc taaacgatct aaaacaaggg ataagccgac
      421 gatataaatc gtgatctcga ccatactgac aagtggagca atcagtcgat agcctttcat
      481 tgtcaacatg aaacgtaacg tgttcagagt gatataagca aaattaatga ggaagatcat
      541 ccccaacatt tttggatcaa tcatttgtaa aactcctttc atttatataa caacaatatc
      601 aaaaaaaatc gtaagtgaag aaaaattgaa actttataca tacaaaaaaa tgataaaatg
      661 attataatag aattataaag ataggttagg tgattaaaat gaaaacggtc aatcgtgtgt
      721 tttgtgccag ccacgtgcta agtctccttc tcagtatata cttaggtgtc tattcgattc
      781 aacagccaat tgaggtagag aacattgttt ttcagatgtc gctgacagat ggtttaattt
      841 taagcgtttt tttctttttg atactgttta ttggtaatat tttcggtagc gttgccagtt
      901 ttttgaattt ttctatttat ccactccttt cgctagcatt aggagtggtg ggactttgtt
      961 ctctgtgctt atttcccgag ccattcagtc ctgcatttat attatttggt acactgaacc
     1021 tgttccagac aactgttgga gcatggttgc tatggcgatc aggaaattta atgaaaatag
     1081 gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt ccagtgttag
     1141 aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat tatcacggat
     1201 ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag cagggcattc
     1261 gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt tcttctattc
     1321 catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac acacttatca
     1381 agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg gtttcgatgg
     1441 ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg ggcgtgtgtt
     1501 tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc gcatcggaat
     1561 ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag agctatgatt
     1621 tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat ggacaagctg
     1681 acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg cataagtctt
     1741 acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta caaataccac
     1801 taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat aaataattga
     1861 aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg ctataataaa
     1921 ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta gaaaggaaga
     1981 gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa tgacggtctc
     2041 tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag ttttctcagc
     2101 aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca cgaatcggtc
     2161 gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct attacatgaa
     2221 tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc aattagttac
     2281 aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg tcagaaaaaa
     2341 agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg aaaattctga
     2401 aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa cgcaatatgc
     2461 cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg agccttttga
     2521 aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc ctagtaaaat
     2581 cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa actgggcaga
     2641 ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag gagcagtaag
     2701 agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg gatttgatgg
     2761 ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag atattatacg
     2821 tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag gggcgagtca
     2881 aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt aagtcttatt
     2941 a
//
LOCUS       DACCWV010000208         2925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCWV010000208 DACCWV010000000
VERSION     DACCWV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.213"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..763
                     /locus_tag="IVW84_002790"
     CDS             <1..763
                     /locus_tag="IVW84_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7918941.1"
                     /translation="EKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRIQPEYELFK
                     RLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTA
                     TAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVD
                     QFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQ
                     TIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            779..1819
                     /locus_tag="IVW84_002791"
     CDS             779..1819
                     /locus_tag="IVW84_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7918942.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1838..2923
                     /locus_tag="IVW84_002792"
     CDS             1838..2923
                     /locus_tag="IVW84_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7918943.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         1070 a          423 c          551 g          881 t
ORIGIN      
        1 tgaaaaaaaa ttgtttttga atacaacatt tgaaaacttg acattgatca atccaaatat
       61 tatgaatttc gaagatgaaa gtattattga agcatctgga tttagaatcc aacctgaata
      121 tgaactattc aaacgtttgt ttgatatcat cgtttcactt gttttattag ttattgcttc
      181 accatttatg ttattcacag caattttagt caaagtgaca tcaccgggac caatcatcta
      241 taaacaagtg cggatcacaa aaaatcaaaa ggaattttca atctataaat tccgctcgat
      301 gaccgccaca gccgaagcaa aatctggacc agttttagct aagagcaatg atgcacgagt
      361 aacgcctgta ggtaaattta ttcgagctgt ccgctttgat gaattaccac aaatcttcaa
      421 tgtgctgaaa ggcgatatgt cgatcgtagg accacgtcct gaacgtcctt tcttcgtcga
      481 tcagttcaat gcagaaaacc cttattacta cttacggcat aatgttcgtg ctggtatcac
      541 tgggtatgca caagtctatg gaaaatatgt ctctgattac aacagtaaat tacgttttga
      601 tttactttat atcaaaaaat attcattgat gcttgattta aagatcttgc tgcaaaccat
      661 caagatccta tttgataaaa tgtcctctca aggacttgaa gaagacgaaa atgtgatctc
      721 attggcagaa attgcttttc cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat
      781 gaacaatccg aaattcagca taatcgtacc agtatacaat gcagaagcaa ctattggtcg
      841 aacgattgat attctaaaag aattggatta tgaagagtac gaagtcgttt tagtaaatga
      901 tggatcaaca gatcaaacac aggaaatcat tcaagaactg attgaaaatg atgaacgttt
      961 tgaattgatt actacgacca atcaagggcc cggattagca agaaacgaag gaataagaca
     1021 gtcaaaagga gaatatctct tattcttcga tgcagatgac acgccagtaa aaacgatctt
     1081 gtcagattat gatcatttgc tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt
     1141 tgtttttcgt acaatggacg agggaagaat cgtttccgaa aaagaaaact tagttacaga
     1201 atgtcactat ctaactcacg aagagtttat aaaagacatg tatcagttaa tgaatgaaca
     1261 gttgatgtat gttgtatgga acaaatgcta tcgtagcgat attctgaaaa ataaccaaat
     1321 tctatttaaa acttataata gttgtgagga tcgaatattt aacttacatt attatgaata
     1381 ctgtcatcaa gtgatcatga acccaaaagt agaatatatt tatgaatttg aaggtgggaa
     1441 ggggatcact aatcaatacc gacctaacaa gttcgaaaca tttaaagaat tctattcatt
     1501 agcaaatact gtaacagacg atattaataa agacggtatg gcggcgcttc ttttaaaagg
     1561 gacaacttca gttatttttt ctatttatag tacctctgca agaacttcaa aagagaagaa
     1621 agacgaagca aagcagatat tgagtgatcc agtgatccga gaagccaagc aaattgctaa
     1681 gacggattct tcagcaaaaa aaataacaaa gttgctttac aatatgccag ctccactatt
     1741 tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa atgccagggt taatggctgc
     1801 tttgaagcga gtatattagt taatggagga aaatataatg aaaagaattt tgcattttca
     1861 gggacgaatg ggtttaggtg gagcagagtc tttcatgatg aatttatatc gtaaaattga
     1921 tcgtacaaac tatcaatttg attttttgat ttatgaagat tatgcagatg ttcaagacta
     1981 tcattcagag attgaaagat taggaggccg tatttttgtt gtacctaatc ctaagaaaaa
     2041 tatttttaaa tatttaatag aagtgaataa gttactaaaa aaagaatctt tttctatagt
     2101 tcacaatcag gtttattttg gcgggggaat taatttatgg ttagccaaaa agaatggaat
     2161 cagacagaga attgctcata gccatgcaac tgaagatgga aaatctcaaa atattgtaat
     2221 gaatgtattg cgtaaatttt taaccaaatt gttgttacaa aatgcaacag actaccttgc
     2281 cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccac ccctttgaga tagttcataa
     2341 tggtattgat ttagagcttt atagtaaaaa ttcagaagct aaggttaata agagaaaaga
     2401 attggatatc tcaatgagta cttttgttgt gggaaacatt ggacgattag agaaacaaaa
     2461 aaatcaatct tatttgttag agattttttt agaaatacta aaaatgaatt ctgattctta
     2521 cttgatttta gtaggtgaag ggtcacttag aaataaactg aaagccaaag cagtagaatt
     2581 ggatatagag aaaagagtaa aatttttagg agaaagaaac gatattcccg agttattatc
     2641 tgtgatggat gtttttgtaa tgccttctct ttatgaaggt ctgccaatgg ttggattaga
     2701 agcacaagca gcaaaattaa aattagtctt agcagatact atttccaaag atacaaagtt
     2761 aacaaataat gtggattttg tatcattaga tgaaacacca caagaatggg caaaaaaagt
     2821 aataaaagag ccgtatgcta ataaaataac acaagaacta aagagttatg atgtttcgta
     2881 cactactgaa caaatggaga gtatatatag tagagatcat taaaa
//
LOCUS       DACCWV010000209         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCWV010000209 DACCWV010000000
VERSION     DACCWV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.214"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(119..481)
                     /locus_tag="IVW84_002793"
     CDS             complement(119..481)
                     /locus_tag="IVW84_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918944.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(555..935)
                     /locus_tag="IVW84_002794"
     CDS             complement(555..935)
                     /locus_tag="IVW84_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7918945.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1017..2192
                     /locus_tag="IVW84_002795"
     CDS             1017..2192
                     /locus_tag="IVW84_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7918946.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2269..2853)
                     /locus_tag="IVW84_002796"
     CDS             complement(2269..2853)
                     /locus_tag="IVW84_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7918947.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          889 a          554 c          482 g          932 t
ORIGIN      
        1 aaataaaatg cactaaaaaa ctatctttag cagaacattt cattgtattt tttggaccaa
       61 acaataaaaa aagaagtttg aaaaaaattc aaacttcttt gttcgcttaa tctgtatttt
      121 agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt tgacctggtt
      181 cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca gtttcaaaga
      241 caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc aaactaatct
      301 tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct aaaccagtct
      361 ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa tggaccaaaa
      421 attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt ttgcttgcca
      481 aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc acattttcag
      541 aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa tgcttcttct
      601 atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt tttattcgtg
      661 aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt tcttaaagat
      721 aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac tggctgtccg
      781 acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga aacatgaata
      841 agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat ccctgtcact
      901 tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt cgcatttatc
      961 attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt cttaagatga
     1021 tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg gacgcaattg
     1081 ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg gacttcttgt
     1141 ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc tttggttatg
     1201 ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa caccatgctg
     1261 tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt ttagctgcag
     1321 cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca gtttatcctc
     1381 cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct ttgctagaaa
     1441 aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa gaaaacaatg
     1501 taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg agcaaagaag
     1561 aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt agtgacgaga
     1621 tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg attgatccta
     1681 atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat ctagctgcaa
     1741 tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt gaaaaacatt
     1801 tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc caagcggctt
     1861 atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa aatgttcaaa
     1921 cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac cctgaaggaa
     1981 cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa ttggagaaca
     2041 ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt ccttccggtc
     2101 atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa ggacttaatc
     2161 gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa aaggacgaga
     2221 ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact attctgaaat
     2281 ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac gttgtacatt
     2341 tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg tatgtcggaa
     2401 atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg ggaatccagc
     2461 accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa taaagaattg
     2521 gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa ggttgaacag
     2581 ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac ccattcctgt
     2641 tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa aaatcacgcc
     2701 atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg gtgctagttc
     2761 agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt ttggtccttt
     2821 ttcacttttt aaatctagtt gtgggaatgc catattg
//
LOCUS       DACCWV010000210         2718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCWV010000210 DACCWV010000000
VERSION     DACCWV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.217"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(87..1034)
                     /locus_tag="IVW84_002797"
     CDS             complement(87..1034)
                     /locus_tag="IVW84_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7918948.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1039..1791)
                     /locus_tag="IVW84_002798"
     CDS             complement(1039..1791)
                     /locus_tag="IVW84_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7918949.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1792..2700)
                     /locus_tag="IVW84_002799"
     CDS             complement(1792..2700)
                     /locus_tag="IVW84_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7918950.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
BASE COUNT          886 a          516 c          340 g          976 t
ORIGIN      
        1 tctatcccag atttgatttt acaaaactca tcagtcctta ttgacaaaga gcctgaattt
       61 accctctaat cttatcttag tatgttttaa ttgatttcag ttttcaaata attagtcatt
      121 ccacttaaaa taccatattt cggttgatag ccaattgact ggatcttagt aatatctgct
      181 agagagtcat ggatatcacc cgaacgctct tctttgtatt caagtgtcaa ttctacaccg
      241 agaatttcat tgattgcatg aatcaattcc aaaagtgtgg ttgctttacc cgttccaaca
      301 ttgaattgtt tccccagtgc agcttcctca ttcgcaacta aaagtaatgc ttggattaca
      361 tcatctatat aaacaaagtc tctagactgg cttccatcac caaataatgt aaatgaagtt
      421 gcttctccta cgatttgttt cttatagcga tctacgagaa tagaaatcac accactgtat
      481 ggagaattcg gattttgatt tggaccatag acattgaaaa aacgaactgc gctaccgggt
      541 acatcatata aatgacaata atctagcaca tattgttctg cagcgaattt atcaaccgca
      601 taaggcgtca atgggcgaat cacggactct tctttttttg gtaaagtcgg ttcatcccca
      661 taaacagccg ctgaagaact aaacacgatc cgttttaaat caggttgata cttgcggatc
      721 aattctaaaa tcattaatac actttcaaaa ttcacacgat gtgtttctac tggtcgggca
      781 actgaatctg ccacactagc aactgctgct aaatgaaaga tataatcaaa tttattagct
      841 ttcatgatct cttccatcaa ggatttctca gcgacatctc cctcaataaa agtgatattt
      901 ttcgtcatat caagattttc tacttttccc attgataagt catcgacgac gattaccaca
      961 tgttcttctc ctaaatgatt ggctaaggta gaaccaatga agccagcccc accggtgata
     1021 agataacagc tcatcctttt acctccgata taaataatta tagatatctt gaagctccga
     1081 agaatcttct aaaaatttag gaaacaaaaa ttctaaccat ttaaaatctt cttcactatc
     1141 aatatctaaa attagataat ctctcatttt tattatccca cattgcccat caaaaatata
     1201 attacttttt tttagaaatt caggtttaaa agaatacatt gaggcattca tttcaaaaac
     1261 ttcaggtgtc tgttgcctag atgtgtatct tgatttatta actatagtaa cttcattttc
     1321 aattcttttt accatattaa aatagggatt acgtcttgca gaaacaactg aaaagtagac
     1381 gtctttatct aaatttttta gatgttcatc tattaattct tcaagattag aaacttcacg
     1441 taaaggtgaa gtcaaatcta aatctataat taaatcaaat atattatctt ctctttctag
     1501 ttgtaagaat gaatcacgta tcacttcaat tttccctaca gtatctgtag ctaattcagg
     1561 tttacgttct acaattttca cagacggatt taacttagtc gtcaattcta ctaactcatt
     1621 actgtccgta ttcaaacaag tcgtgcaatt atgttcagga tgtttttgtt gatagatatc
     1681 tatcattgct aaagcgaaat ggactagggg ctttcctgcc aaatatttca aatttttatt
     1741 tttaaacccc ttagacccag ctctaccaca aatagtaaat aaaatttcca tttatttctc
     1801 ctcagctatt ttcaatactt cgtttgcatg atataaatca ttccagtttt ctttgcctgt
     1861 tactacgttt tcaaagaaat atttcaactc cttctcatac atatcattag cagattcagg
     1921 catttcttta acaatgtttt cttcaaaact tattttatta tttaataagt caacattgat
     1981 acattcatct aaagtaaaca attgaaattt tcttgtagga actcttccaa aataatctaa
     2041 atgaatttct ataattttat catcgtatct caatatatat gttgctaaat cattactgtt
     2101 aatttcaaga ttcgaatatt taccaaactg tttttttact gatttaggga aaccaaatag
     2161 gtttataata taatccaatt catgtataca gtctaattct actcctcccc ccatagattt
     2221 aaaggcagaa taattatttc tataatcagt accttttctc caatcaggaa gataagtaga
     2281 acaaatagct cttacagaat atactttttt actttgaata ctattacgaa caacttccat
     2341 gatattttta tacctcagtg gagcagcaac ataaatattt ttgtaatcat aattatattg
     2401 agtaagatca atactctcag aaaaagcagg tttttctaaa aatatgtaat caactttttc
     2461 aacaattaca tttagtgttt ccagatgctt aaaagtcgga tttgtaataa aagctatatc
     2521 ataatgtgta tccaaatcgt ctctattttg aatatacgtt atttcttcaa actctttttt
     2581 tccactgcta ctataagctg ttatatcaaa attaatagaa ttcttttttc catagcttat
     2641 tagatttttt agatgtctct ttccaattga ccctaaccca aaaaaaatta ctttcatcat
     2701 taatttacct caatgtcg
//
LOCUS       DACCWV010000211         2624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCWV010000211 DACCWV010000000
VERSION     DACCWV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.218"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..189
                     /locus_tag="IVW84_002800"
     CDS             <1..189
                     /locus_tag="IVW84_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918951.1"
                     /translation="ADLYLKDTGVLIGTNSKVYQEVKEKIEAKLNPSNRNRPKNHQKE
                     TSQTVTSRNFDPTNFLKR"
     gene            complement(326..1045)
                     /locus_tag="IVW84_002801"
     CDS             complement(326..1045)
                     /locus_tag="IVW84_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7918952.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            1720..1815
                     /locus_tag="IVW84_002802"
     CDS             1720..1815
                     /locus_tag="IVW84_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7918953.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2117..2362)
                     /locus_tag="IVW84_002803"
     CDS             complement(2117..2362)
                     /locus_tag="IVW84_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918954.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          839 a          497 c          413 g          875 t
ORIGIN      
        1 gctgatttat atctaaaaga tacaggcgtt cttattggca caaattctaa agtatatcaa
       61 gaagtgaaag aaaaaatcga agctaaacta aatccatcaa atcgaaatcg accaaagaat
      121 catcaaaaag aaaccagtca aacagtcact tctagaaatt tcgacccaac aaattttcta
      181 aaacgataaa aaacagatag gagttattcc tatctgtttt ttatttagaa gccccataca
      241 atatgctaga atgtactagc aatcttagtc ggttgtaagg gacttgtcta cttgcttaat
      301 gtaagggggg cgagttcttt tttatttatt cttctaacca gttacgcaga gaaatctttg
      361 gcaaatctga cgttaggctt gaaaaagtaa attctaattt ggcgatttta ttgccctttt
      421 ttgcttttat ttttgtgact ttaagatcat tgaagtattt ttttagttct cgtaatgcag
      481 gatttaatac tttttgatca atgttaccca tttgatagta ttctgggata tccattaagc
      541 tcctaaactc ttcaatacga actacataat aaccagtaga acggtactgc ataagcagtc
      601 ggaatagagt ttttgcatac gtagaacgca acgacgtgaa agcagataat tcaaatctac
      661 taaattcagc tgttaaacca ttaataatat attctaggtc cggattaaca ctgacttcaa
      721 caaacttttt tgttttattg atttcaaaac cagtaaacaa aacaaagtag cgaatagaat
      781 cctcatcttc agtacggtaa gtaagattaa gcatatcttt atagagcttt tctaaatcac
      841 cgacaaaggc ttttgtagca gtcatcttat aatcacttaa ctcttttaat tcctcgaaag
      901 aaaatctaac ttttttcaaa ccacgatctt tcatacgagc gcataatgca aaaaataaat
      961 ccatctgctt agcattgaaa tttttcagcg gtactaaatt caattcattt tgatatttga
     1021 ctgttaattc cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct
     1081 ttaacaacca actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga
     1141 taaaaagttg ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta
     1201 ctcagataaa aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt
     1261 gggagagtaa ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat
     1321 aaaataaacg cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat
     1381 aaacaggttc gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag
     1441 gagtgatcgc tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa
     1501 aaataattca ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga
     1561 caagattcga taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag
     1621 ccctaacaac cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat
     1681 tgattgtcgc ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac
     1741 tttatttgct cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac
     1801 cgaaacaaga agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat
     1861 ccgacctctc tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca
     1921 gaaataaata tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta
     1981 tatgcaacat ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt
     2041 tatttagttt ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag
     2101 agccatttaa acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg
     2161 cgtctgaaat tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc
     2221 tttcgggtgt catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg
     2281 cttgaattgc ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca
     2341 ttttttcttt ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat
     2401 actctcaaaa aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat
     2461 ttggttatcg attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt
     2521 gtacccactt tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa
     2581 aatttgatat cactttttcg ccatcgtggg tagcttatgt atag
//
LOCUS       DACCWV010000212         2607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCWV010000212 DACCWV010000000
VERSION     DACCWV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.219"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(54..788)
                     /locus_tag="IVW84_002804"
     CDS             complement(54..788)
                     /locus_tag="IVW84_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7918955.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IVW84_002805"
     CDS             1115..1525
                     /locus_tag="IVW84_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7918956.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..1718
                     /locus_tag="IVW84_002806"
     CDS             1518..1718
                     /locus_tag="IVW84_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918957.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIELNPKS"
     gene            complement(1720..>2607)
                     /locus_tag="IVW84_002807"
     CDS             complement(1720..>2607)
                     /locus_tag="IVW84_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7918958.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          796 a          444 c          445 g          922 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatattgaa ctgaacccca aaagttgaac tatttaatgg actgtttccg
     1741 atattctact ggagatagtc catttaactt tagttttatc cttttgttat tataccacct
     1801 aatatactca tttaatttac tttgaaatat ctcaattgat cggaattttt ctcgataaaa
     1861 aaactctgat ttaagcacac caaaaaaatt ttctattaca gaattatcta agcaatttcc
     1921 ttttctagac atgctttgaa taatgttatt ctcttttaat tttttttgat attgtggcat
     1981 ctgatactgc catccttgat ctgaatgtag aatcagtgaa ccctcagttc cctttttttt
     2041 aattgcttgt tgaagcattt cttcaatcag tttatatgtt ggactagttg atatactata
     2101 actaattatt tctccgttaa ataaatcaag tataggagat agatagattt ttcttccttt
     2161 tatcttgaat tcagtgacgt ctgtcaccca ttttttattg ggtgtatcta ctgaaaaatt
     2221 tcgttttaac acattcttgg caatttttcc tactgttcct ttataagatt tatatcgctt
     2281 tattcggatt tgacaggtaa gtcccatttg ggacattaat tttctaactg ttttatgatt
     2341 gattgtatat cctttcatct ttaacgctaa agtgactcta cgataaccat aagagtttct
     2401 tgattctttt acaatcgctg taatttcttg ctttatcttg ctatatttat ctggcttatc
     2461 taattttttt acccagtaat aataagttga cttcgctaat tgtgcaattg aaagtaatag
     2521 attcaattta aattcttttt tgagctgaag gattgtttta accttgattt cttcttgctc
     2581 aaatcttgtt cttgaatcaa ggtttct
//
LOCUS       DACCWV010000213         2604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCWV010000213 DACCWV010000000
VERSION     DACCWV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.220"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            100..2541
                     /locus_tag="IVW84_002808"
     CDS             100..2541
                     /locus_tag="IVW84_002808"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7918959.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          872 a          460 c          614 g          658 t
ORIGIN      
        1 taaggattgt taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt
       61 acagcaaaga atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata
      121 gatgctgcaa gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt
      181 gaattaggca cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa
      241 atcgatcaag taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga
      301 cagattgctt tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt
      361 tgcggatctg gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca
      421 gaacttgtga tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat
      481 cagtcagaaa caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact
      541 gacgcatttt caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct
      601 gtgagcagag aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct
      661 gtcgaagccg gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg
      721 ttatctgagg atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt
      781 acagtattct cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt
      841 gcctctgtgg tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta
      901 gcaaccatca aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca
      961 atcaaagcca ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt
     1021 tttgagatca atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta
     1081 gatgaagaaa aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca
     1141 agcggtgcac gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac
     1201 ggtattgctt cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat
     1261 atggagcaaa cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa
     1321 cggcgcagtc aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag
     1381 gaacaaacac tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg
     1441 gaaatcccaa tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg
     1501 atggcaacag aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga
     1561 aatatctgcg cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa
     1621 tcggagtatc aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt
     1681 gcaaatgaaa gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact
     1741 cgggaattca tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa
     1801 gatgcaatgg gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg
     1861 gagtggttcc ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca
     1921 ttggcttctg cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt
     1981 gaacaaattg ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt
     2041 gcagcaacgc acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga
     2101 aatgatacac gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac
     2161 caaggattaa cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct
     2221 ttggctgttg ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta
     2281 gctatgttag atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta
     2341 gctcaaaacc tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg
     2401 ggacttcaag ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa
     2461 gttgcgaaaa aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg
     2521 gaaaaaatcc gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag
     2581 ttattttatt tactgcagat cttg
//
LOCUS       DACCWV010000214         2593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCWV010000214 DACCWV010000000
VERSION     DACCWV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.221"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            31..>2593
                     /locus_tag="IVW84_002809"
     CDS             31..>2593
                     /locus_tag="IVW84_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918960.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT          832 a          470 c          625 g          666 t
ORIGIN      
        1 ttgtcaaaac gtcagagagg atggtggaga atgaatcaat atgtaaaaaa gctgattgcg
       61 tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa
      121 acaagcgaat caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa
      181 tcaattactt ctgaaactac agaatcccaa agtgaggcgc ctacttctaa tacaaggcaa
      241 gaagaaaata agacaagtga gacctcagaa gtagctgaac aaacgtctga tagcagtgaa
      301 gaggtacagg ttagagttgt ttcagcggat gggtacagcg aagctgccac aacagaagaa
      361 ctgacgcagc ttctcgcaga cgaatcagta gctaagattc gtttaactca gcctttgact
      421 ttagacaggg aattggagat caagcgagat atcgttatcg attttggcgg acttgctcat
      481 aatttcggca cacatcacat ctatatcaat gagacacctc agtcgatcga gtttcagaat
      541 tttaaaggaa cggcagctca tccaggtggg ttgataccaa atatcgatgg aaatgccatt
      601 atcattgctt atatgtccga tttttgggga aacagatatc attttacggg agaaataaaa
      661 tttaccggga cacttgactt atatgatggt tctaaattag gcctgattta cgcccctcgt
      721 gcaactgtca cattagatgg tgtttccggt gtattggatg tccaaccagt gaaagaaggg
      781 atgaatgccg ctccgggtaa agcttatttt tgtagaagct atcagctaaa tgtaatcaat
      841 ggtagtcagc tttatggacc atatctagga aaattctatg gttttctaga ttcaggtgat
      901 gagacgggca gtggaaaaac agcaccagga ctccatatct tgagtggttc aaaggtctct
      961 cttgactatg atcgggcaga tggcgtcagc gatgaaggag aggctgtcga tacgctaccc
     1021 gggaatgtca catttaaagt cagtggctca ggaagtgaat tttcggtaaa tacaaagatc
     1081 aatattacca acgacaacaa tcgcggaatt atccaaatga gaggctcagg atcaagagtc
     1141 gctgtgtcga atgatgggaa aatcacaatc aatacagcga ctactggcgg atttcgcctg
     1201 caaggagatt ctagtcaaat ccatgtatca agtggtggac aaatctcggt caaaatggct
     1261 ggagatggcg accaacctgg aaacaatggg atgcgttttg tagggaaagg actttctttg
     1321 atcgtagaag gagccggatc agtcatcgat attgataagc aaagtggtcg ttctagcgca
     1381 gttcgttttg agaacggtac gcaaaaacta acagtaacta atggaggctc attgaaggta
     1441 agaaatgcag gggatggcaa atcttatgtg aacagactca atcaaggaaa ccaagcgatc
     1501 caattttatg acgcttctgg ttttctagaa agtccagggt cagctgattt tacggttact
     1561 ggagaaaaat caaatatcga tatacgcgca gattccggag ctaccgtaga tacaactgga
     1621 gatattgatt tgaactttat ggctggcgaa aaaacttatt tagtcatggt cgggaaaact
     1681 ggggaagata agactggcat catcagtggg gatgtgttga atgtagactt agaatctcct
     1741 acctatttcg atttccaaaa caaacggatc ggaagcgaga ctaatggcat aggtggctgg
     1801 gtatttcaag gaaatggcga ttcttctttg aagatcgctc attcagaaat tgctttatgg
     1861 cgaaaggcag gagtaacgtt cgataacttt gaaaaagatc cggattattt ttctgatatg
     1921 acagacttat cgatcaatgg aaccgactta ggtaattttg tatcaagccc tagtgcaggt
     1981 ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa attataatcg aatcagtgcc
     2041 aataaccagc ttccgatgat tgaatcctta agagtaccaa cgaatgcaga taaaaaaatc
     2101 tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc caagagatgc ttggacaggc
     2161 gaagtagaag tgactttacg tattacttat gcagatacga cgaaatcacc tgtagaaatt
     2221 actgctctca cacaaggaca aacagatgaa aatgaaggga caggatataa tccgtgggga
     2281 gaaggcgaac aaggcggttt gtttgaggtt caagtaccca acgaagaaag actaacaacg
     2341 ggggatcaag tgcaaatagt tgctgctaaa aaagtaagag gcggccaaga agtgacagaa
     2401 ctggactctg ccaaaacgac agtcgatgtg attcctcctg agcctgctaa attaagtacc
     2461 acattgatca atgcagcttc tcgctcaatc acaggtacag gcgaagcagg agctttggtt
     2521 tcattgaaaa aaaatcaagc tgttttgtcc gaaacaacta ttgatgatac aggtaaattt
     2581 gttctttcta tcc
//
LOCUS       DACCWV010000215         2580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCWV010000215 DACCWV010000000
VERSION     DACCWV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.222"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..200)
                     /locus_tag="IVW84_002810"
     CDS             complement(<1..200)
                     /locus_tag="IVW84_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918961.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAK"
     gene            complement(212..412)
                     /locus_tag="IVW84_002811"
     CDS             complement(212..412)
                     /locus_tag="IVW84_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7918962.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            487..1137
                     /locus_tag="IVW84_002812"
     CDS             487..1137
                     /locus_tag="IVW84_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7918963.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1192..2358
                     /locus_tag="IVW84_002813"
     CDS             1192..2358
                     /locus_tag="IVW84_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7918964.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          922 a          430 c          390 g          838 t
ORIGIN      
        1 tatttagcca tctcttttcc ttttagaaat ggtctttcta atccggtatc aattcttgct
       61 tgatcaatag cctcttttgt catttcaaaa acataatttt tcaattgatt cgtttgttgt
      121 ttatctaaca ttactgtaat tcctgcatcc attatttttc tcctctcatt ttctatttct
      181 tcaatcagcc aactcgccaa gttgtattta tttagatttt caaatcatta agtaaccaat
      241 ttaccaactt ttgataagta gatgctctaa tactttcttt gctcccattt tcgattttga
      301 catatgtttt ccgatttata cctatcaatt ttgctgcatc gtttttagta agtataagat
      361 cggctctatg tcttcttaag cttctaatga attgatcatt aagagttatc attcagcgcc
      421 tcctttcaaa ttattcactt tgtggttttt atcttaataa gcacatttta aaccacaaaa
      481 cgagtaatat caattaaaat attactcaat ttgtggtttt catgctataa tagtttggaa
      541 tttactatag ggggcgttac tttgaataga ataaaatttt atattgattc cagtaaaaag
      601 accattaaac aaattagtac agaaactggt attaactata caactcttac gaactattac
      661 cgtggtgagc gaaatcctag aaatactgaa acatggaata tccttgctga ttatttccat
      721 gttcctgtaa gttatttgat gggattagac gaaacaaatt ccatagaaca acctaatctt
      781 gatagaataa aaactgctcg tttagaaaaa aaggacaaaa tagtcaagaa tattgccagt
      841 cttgactcag attattttac tgaattatct aatctatact tttccatgac ttctttactt
      901 gaagtcaaac ttcaagaaga tgactccgac tttctttttt taaaaagtat tctagaggac
      961 tcctttgaat acacgattct atttgccaaa caaaagaagt ttattaataa agctttaaac
     1021 ttaaatatcg atgaaagaaa agagttagaa tctgagttat ttggcaactt aacccaagta
     1081 caggttaaca atctaaaaag agagataaca gctcgattaa tatcaaaact tcaataaaac
     1141 taattattca atcagccaac tcgccaagtt atcagattgg agagattagt tatgtcaata
     1201 caaaagtaca caacaaaaag cggtgcacga tatagtgtcc gcttgtatgt tcgtaaagat
     1261 gaaaatggaa agcaagaata ctatgtaaaa aaagggttta aaactgaaaa agaagccaag
     1321 ttacacgaag caagaaaaaa ggtcgagatt gaagataccg gctatcataa gccttctaat
     1381 gacttgttta taacagtgta tgaagaatgg ttgccttctt ataggaatcg tgtacaagaa
     1441 acgacatatc aacgtacaaa agaccttttc cgcttgcata tactaccaaa attcgggaat
     1501 aagtccattt ctaagattac tcctgttcac tgtcagaatg cagtcaatga atgggcttta
     1561 aaattcaaga atatcaaaca gcttaagtct tacagctctc aaatatttga gtatgccatt
     1621 tttgcagagc tattacaccg aaatcctatg gcaaatacaa atctaccaaa gcgggaaaag
     1681 aaagaaaatg aaaattactt ttcattagaa gaactaaaag ctttttggga tattctcaat
     1741 aaagaagagc ctttaaaaca tattttaatc ttccagcttt tgattactac tggtatacgt
     1801 aaaggagagc tagctgctct tcattggtct gacatttatt ttgaagaaca gctaatgtat
     1861 gttagaaaga gctatgcaac tattcgaaat gaagagaaga atgcaagaag aaaaactgtc
     1921 aggattcaaa aaaatacaaa aaatatatct tccgaacgaa tattaccaat tgatgcacaa
     1981 actattgaaa tgttaaaaat ctggaaaaaa gaacaagctc gagagctttt gcagtttggc
     2041 ataaatacaa atacaaaaaa tcaacttatc tttacctatg taaatgcaga cggtgacatt
     2101 aatcaacctt tgcatgcgga ctattcaaat aatatattga aacgtttaga gaagaaatat
     2161 aaatttaagc acgttactat tcatggattc cggcacactc acgctactct tctactagaa
     2221 ggtggagcaa gtatcaaaga aacacaagat cgattgggtc ataaaaatgc tgaaactacc
     2281 cttaacactt attctcatgt tactgaaaaa gctcaacgaa atgcgttaga aaaattcatt
     2341 gcttacactg gtttataata ttgtcattac ctttctcatt accagaacga aaaaaagaca
     2401 ccttctcgca atgagaaagt gtctgaaatc gttggtataa caacgattaa cgttttgaaa
     2461 actgtgaagc tttacgtgct ttcttaagac ctggtttttt cttcgtatca ttgaataagt
     2521 ttgaaaagcc ttgatttata aaggtttttc cgtaaatata ttttgttata tttgattaaa
//
LOCUS       DACCWV010000216         2460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCWV010000216 DACCWV010000000
VERSION     DACCWV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.223"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..420
                     /locus_tag="IVW84_002814"
     CDS             <1..420
                     /locus_tag="IVW84_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7918965.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTET
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(744..827)
                     /locus_tag="IVW84_002815"
     tRNA            complement(744..827)
                     /locus_tag="IVW84_002815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(791..793),aa:Leu,seq:cag)
     gene            1355..1855
                     /locus_tag="IVW84_002816"
     CDS             1355..1855
                     /locus_tag="IVW84_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918966.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2045..2389)
                     /locus_tag="IVW84_002817"
     CDS             complement(2045..2389)
                     /locus_tag="IVW84_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7918967.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          767 a          471 c          407 g          815 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagttcaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa cacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag tacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggcc gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattat ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt
      481 ctttcgcatg acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc
      541 agtatttctt gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa
      601 ctgtcccacc ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa
      661 tttttcctat attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct
      721 tctctttata aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc
      781 tcactagcgc ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta
      841 gtttaggatt acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac
      901 gccctccctt atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg
      961 gttggtgaaa aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg
     1021 ggattagtaa ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc
     1081 tatcttcttt catttggata ttgattattt cttctgttga ttactatcct tctggaattc
     1141 tagacgctga agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga
     1201 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
     1261 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
     1321 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
     1381 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
     1441 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
     1501 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
     1561 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
     1621 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
     1681 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
     1741 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
     1801 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
     1861 aaggattcca aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat
     1921 taacttataa tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc
     1981 tagaaaggat aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag
     2041 atttctatcg tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg
     2101 ctggatacca gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt
     2161 catggataga ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct
     2221 taatgactgt ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga
     2281 cgatgtcgat tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt
     2341 tgattagttc tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact
     2401 ccttaacacg atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag
//
LOCUS       DACCWV010000217         2430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCWV010000217 DACCWV010000000
VERSION     DACCWV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.224"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..189
                     /locus_tag="IVW84_002818"
     CDS             <1..189
                     /locus_tag="IVW84_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918968.1"
                     /translation="ADLYLKDTGVLIGTNSKVYQEVKEKIEAKLNPSNRNQPKNHQKG
                     TNKTVTSSNFDPTKFLKR"
     gene            complement(277..999)
                     /locus_tag="IVW84_002819"
     CDS             complement(277..999)
                     /locus_tag="IVW84_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HAP7918969.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMD
                     EKTNKKDWTSSDVIRNGKHYVKPHYHVLYIARNPVTIESVRKKIKRKLGNNSVAHLEI
                     IDHIKGSYEYLTHESKDAKEKNKHVYDKKDILHINDFDIDRYVTLDESQKKELRNQLF
                     DLVNHCGIVNMKELAAFLDWRGQEYGITNQRDVVDVIASNSNLFRMMFDANYQNGFRP
                     QYAKRIDSETGEMIEGVNKHVE"
     gene            complement(1080..1229)
                     /locus_tag="IVW84_002820"
     CDS             complement(1080..1229)
                     /locus_tag="IVW84_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918970.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKK
                     SEKGN"
     gene            1689..1787
                     /locus_tag="IVW84_002821"
     CDS             1689..1787
                     /locus_tag="IVW84_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7918971.1"
                     /translation="MLEKLITLVIAPLFVGLVIELISHWLDEKDDN"
     gene            complement(1801..1977)
                     /locus_tag="IVW84_002822"
     CDS             complement(1801..1977)
                     /locus_tag="IVW84_002822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918972.1"
                     /translation="MGNDIPQGLVSLIFQLLLVIIAVWKRDEFLQEQENTKSQPMSVG
                     WDFFCACGGNKRVD"
BASE COUNT          715 a          536 c          364 g          815 t
ORIGIN      
        1 gctgatttat atctaaaaga tacaggcgtt cttatcggca caaattctaa agtatatcaa
       61 gaagtgaaag aaaaaatcga agctaaacta aatccatcaa atcgaaatca accaaagaat
      121 catcaaaaag gaacaaacaa aacagtcact tctagcaatt tcgacccaac taaatttcta
      181 aaacgataaa aaacagatag gagttattcc tatctgtttt ttatttccca atattctatt
      241 ttttcattcc aagcagttaa cggaagtttt cgatatttat tcgacgtgtt tattcacgcc
      301 ttcaatcatt tctccagttt cagaatctat cctttttgca tattgcggcc gaaatccgtt
      361 ctggtaatta gcatcaaaca tcatacggaa aagattgctg tttgaagcta tgacatcaac
      421 aacatctctt tgattagtga ttccatactc ttgacctctc cagtcaagaa atgcagctaa
      481 ctctttcata ttgactattc cacaatgatt aacaagatca aacaattggt ttctcaactc
      541 tttcttttga ctctcgtcca aagttacata acgatcaata tcaaaatcat taatatgcaa
      601 gatatctttt ttatcgtata catgtttatt cttttctttt gcatcttttg attcatgcgt
      661 aagatactca tacgagcctt tgatatggtc aattatttct aagtgagcaa ccgaattatt
      721 tcccaatttt ctcttgattt tctttcttac gctctcgatt gttacaggat tgcgagcaat
      781 ataaaggaca tgataatgtg gctttacata atgcttaccg tttctgataa catcactact
      841 agtccaatcc tttttatttg ttttttcgtc catatcgtgt aacggactaa ccgccattga
      901 tactcctaag ctctcaagct gttctctcca attctctgga atagattcgg gatatagtaa
      961 aaaaccaaaa tgtctagctt tcgttatatt cttattcatg atatacttac ctcaattgaa
     1021 cactatcagt taagcggaaa cggtgcgcca acacatatgt ttctgctctt tttttgcgct
     1081 caatttcctt tctctgattt tttcaagtct tctgttacta gcaatttaac atatgtcgac
     1141 atactcaaac ttttgtcttt tgctttttcc tcaagttttc gcttaatttc actcggtaaa
     1201 attactacca gtctttctga ttccgccatg cctttcagcc cctttagtta attgttaata
     1261 tcatggtaaa cttttgacaa ctgtattgtc aatatttttt tctatttttc aaaacggcgg
     1321 tcaaaacggc ggtcaaatgg cggtcgaaaa aaaaaggctc tatccattgg tacagtaagg
     1381 ataaagcact ttttggcaca aaaattggca ctcggcacac tatggggggg gtcgtagtac
     1441 ggaagcaaaa ttcgcttcct cgccccccag ttgtcccaaa ttccaaattt ttttcaaaaa
     1501 ttttccagcg cttccgctcg gcaaaatcgc aagcgatttt aacactgcct ttttaagggg
     1561 gtgttcaaat tttgaacatt taaccgcctg ttagaaatac ggttaagttt ttcccactca
     1621 ttctcaaaga aaaaaagaga tatgctataa tcacacatgg caacttgccc cgaaaggggg
     1681 tgttctcttt gctagaaaaa ctcatcacgt tagttatcgc accacttttt gtagggttag
     1741 tcatcgaact aatttctcac tggttagacg agaaagacga taactaaagc aagttgcttt
     1801 ctaatccaca cgtttgtttc caccacaagc gcaaaaaaaa tcccaaccta cggacatagg
     1861 ttgggatttt gtgttctctt gctcttgcaa aaactcatca cgtttccaca ctgctattat
     1921 aacaagcaat aactgaaaaa tcaaagacac aagaccttgg ggaatgtcat tccccaaacc
     1981 ccttgatcct cctcctgccg aaatatttct acgctctcgc tcctcatttc attcagttct
     2041 cggctccaca atctaacacg ccgtttcgct accagtcgtc tttcagactc cattctcgct
     2101 cctctcacgt gttttgacga ttgcgccgat aactaaacga aataaacgct aaaacgtctt
     2161 agaacgcaaa atatgagctt ctaagactat tccatttcag ttacatactc tttataatcg
     2221 ttccatttga tttctctttc gtgccattca ttatcgcttt tcgcaaacag aacaacacga
     2281 atccatgtca ctattttttt gttttttaaa gagcataagg aaacgtggta tcactttctt
     2341 acgaaaggtg ctagataggc tgtgcctatc acgtttcttt gaaaaaaatt tgatatcact
     2401 ttttcgccat cgtggatagc ttatgtatag
//
LOCUS       DACCWV010000218         2405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCWV010000218 DACCWV010000000
VERSION     DACCWV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.225"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..103)
                     /locus_tag="IVW84_002823"
     CDS             complement(<1..103)
                     /locus_tag="IVW84_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7918973.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="IVW84_002824"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="IVW84_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..2302
                     /locus_tag="IVW84_002825"
                     /pseudo
     CDS             920..2302
                     /locus_tag="IVW84_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DACCWV010000219         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCWV010000219 DACCWV010000000
VERSION     DACCWV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.226"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVW84_002826"
     CDS             207..2069
                     /locus_tag="IVW84_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918974.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IVW84_002827"
     CDS             2069..>2398
                     /locus_tag="IVW84_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7918975.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACCWV010000220         2392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCWV010000220 DACCWV010000000
VERSION     DACCWV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.227"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            49..444
                     /locus_tag="IVW84_002828"
     CDS             49..444
                     /locus_tag="IVW84_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918976.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            769..1386
                     /locus_tag="IVW84_002829"
     CDS             769..1386
                     /locus_tag="IVW84_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7918977.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1427..1726
                     /locus_tag="IVW84_002830"
     CDS             1427..1726
                     /locus_tag="IVW84_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918978.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1760..1927
                     /locus_tag="IVW84_002831"
     CDS             1760..1927
                     /locus_tag="IVW84_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7918979.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1972..>2392)
                     /locus_tag="IVW84_002832"
     CDS             complement(1972..>2392)
                     /locus_tag="IVW84_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7918980.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          845 a          353 c          453 g          741 t
ORIGIN      
        1 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
       61 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
      121 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
      181 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
      241 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
      301 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
      361 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
      421 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
      481 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
      541 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
      601 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
      661 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
      721 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
      781 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
      841 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
      901 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
      961 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     1021 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     1081 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     1141 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     1201 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     1261 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     1321 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     1381 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     1441 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     1501 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     1561 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     1621 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     1681 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     1741 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     1801 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     1861 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     1921 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
     1981 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
     2041 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     2101 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     2161 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     2221 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     2281 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     2341 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg at
//
LOCUS       DACCWV010000221         2346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCWV010000221 DACCWV010000000
VERSION     DACCWV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.228"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(106..786)
                     /locus_tag="IVW84_002833"
     CDS             complement(106..786)
                     /locus_tag="IVW84_002833"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7918981.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            912..1781
                     /locus_tag="IVW84_002834"
     CDS             912..1781
                     /locus_tag="IVW84_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7918982.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            1807..2322
                     /locus_tag="IVW84_002835"
     CDS             1807..2322
                     /locus_tag="IVW84_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918983.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
BASE COUNT          728 a          438 c          482 g          698 t
ORIGIN      
        1 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
       61 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
      121 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
      181 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
      241 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
      301 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
      361 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
      421 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
      481 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
      541 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
      601 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
      661 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
      721 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
      781 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
      841 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
      901 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
      961 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     1021 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     1081 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     1141 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     1201 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     1261 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     1321 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     1381 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     1441 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     1501 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     1561 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
     1621 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
     1681 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
     1741 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
     1801 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
     1861 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
     1921 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
     1981 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
     2041 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
     2101 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
     2161 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
     2221 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
     2281 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
     2341 cggaaa
//
LOCUS       DACCWV010000222         2295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCWV010000222 DACCWV010000000
VERSION     DACCWV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.229"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(153..224)
                     /locus_tag="IVW84_002836"
     tRNA            complement(153..224)
                     /locus_tag="IVW84_002836"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Glu,seq:ttc)
     gene            complement(236..309)
                     /locus_tag="IVW84_002837"
     tRNA            complement(236..309)
                     /locus_tag="IVW84_002837"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Ile,seq:gat)
     gene            complement(322..392)
                     /locus_tag="IVW84_002838"
     tRNA            complement(322..392)
                     /locus_tag="IVW84_002838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Gly,seq:tcc)
     gene            complement(397..469)
                     /locus_tag="IVW84_002839"
     tRNA            complement(397..469)
                     /locus_tag="IVW84_002839"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Phe,seq:gaa)
     gene            complement(473..545)
                     /locus_tag="IVW84_002840"
     tRNA            complement(473..545)
                     /locus_tag="IVW84_002840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(510..512),aa:Asp,seq:gtc)
     gene            complement(564..637)
                     /locus_tag="IVW84_002841"
     tRNA            complement(564..637)
                     /locus_tag="IVW84_002841"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Met,seq:cat)
     gene            complement(650..739)
                     /locus_tag="IVW84_002842"
     tRNA            complement(650..739)
                     /locus_tag="IVW84_002842"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(701..703),aa:Ser,seq:tga)
     gene            complement(762..833)
                     /locus_tag="IVW84_002843"
     tRNA            complement(762..833)
                     /locus_tag="IVW84_002843"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:Glu,seq:ttc)
     gene            complement(855..942)
                     /locus_tag="IVW84_002844"
     tRNA            complement(855..942)
                     /locus_tag="IVW84_002844"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Ser,seq:gct)
     gene            complement(967..1040)
                     /locus_tag="IVW84_002845"
     tRNA            complement(967..1040)
                     /locus_tag="IVW84_002845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1004..1006),aa:Ile,seq:gat)
     gene            complement(1054..1124)
                     /locus_tag="IVW84_002846"
     tRNA            complement(1054..1124)
                     /locus_tag="IVW84_002846"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1090..1092),aa:Gly,seq:tcc)
     gene            complement(1126..1201)
                     /locus_tag="IVW84_002847"
     tRNA            complement(1126..1201)
                     /locus_tag="IVW84_002847"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1166..1168),aa:Asp,seq:gtc)
     gene            complement(1220..1293)
                     /locus_tag="IVW84_002848"
     tRNA            complement(1220..1293)
                     /locus_tag="IVW84_002848"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1257..1259),aa:Met,seq:cat)
     gene            complement(1306..1395)
                     /locus_tag="IVW84_002849"
     tRNA            complement(1306..1395)
                     /locus_tag="IVW84_002849"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1357..1359),aa:Ser,seq:tga)
     gene            complement(1419..1492)
                     /locus_tag="IVW84_002850"
     tRNA            complement(1419..1492)
                     /locus_tag="IVW84_002850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ile,seq:cat)
     gene            complement(1506..1579)
                     /locus_tag="IVW84_002851"
     tRNA            complement(1506..1579)
                     /locus_tag="IVW84_002851"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1543..1545),aa:Met,seq:cat)
     gene            complement(1607..1679)
                     /locus_tag="IVW84_002852"
     tRNA            complement(1607..1679)
                     /locus_tag="IVW84_002852"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1644..1646),aa:Ala,seq:tgc)
     gene            complement(1699..1772)
                     /locus_tag="IVW84_002853"
     tRNA            complement(1699..1772)
                     /locus_tag="IVW84_002853"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Pro,seq:tgg)
     gene            complement(1782..1855)
                     /locus_tag="IVW84_002854"
     tRNA            complement(1782..1855)
                     /locus_tag="IVW84_002854"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1819..1821),aa:Arg,seq:acg)
     gene            complement(1866..1951)
                     /locus_tag="IVW84_002855"
     tRNA            complement(1866..1951)
                     /locus_tag="IVW84_002855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1915..1917),aa:Leu,seq:taa)
     gene            complement(1968..2039)
                     /locus_tag="IVW84_002856"
     tRNA            complement(1968..2039)
                     /locus_tag="IVW84_002856"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2005..2007),aa:Gly,seq:gcc)
     gene            complement(2051..2123)
                     /locus_tag="IVW84_002857"
     tRNA            complement(2051..2123)
                     /locus_tag="IVW84_002857"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2088..2090),aa:Thr,seq:tgt)
     gene            complement(2141..2222)
                     /locus_tag="IVW84_002858"
     tRNA            complement(2141..2222)
                     /locus_tag="IVW84_002858"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2186..2188),aa:Leu,seq:tag)
     gene            complement(2233..2294)
                     /locus_tag="IVW84_002859"
     tRNA            complement(2233..2294)
                     /locus_tag="IVW84_002859"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2267..2269),aa:Lys,seq:ttt)
BASE COUNT          638 a          622 c          524 g          511 t
ORIGIN      
        1 ttttcatttt tttgcttaaa aaatataaaa atattttaca ttagaactgt ccaaaaaagc
       61 ttagcacaat catccataaa gataactctg ctaagctttt taaaatcatt ctttcagata
      121 aaaagaaaaa gatactcaaa aagtatcttc catgacccgt acgggattcg aacccgtgtt
      181 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaataaa ataaatgggc
      241 ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc
      301 tataggccca taaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga
      361 aggctgtggt tttaccacta aactacaccc gcataatggc gcgggacaga atcgaactgc
      421 cgacacatgg agcttcaatc cattgctcta ccaactgagc taccgagcca aacggttccg
      481 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
      541 gaaccaatgt ttttagtttt taattgcggg agcaggattt gaacctacga ccttcgggtt
      601 atgagcccga cgagctacca gactgctcca tcccgcgata atatgaaaaa ggaggataag
      661 ggattcgaac ccttgcacgg ttttacccgc ctgacggttt tcaagaccgt tcccttcagc
      721 cggacttggg taatcctcca tgtaaaacta tattaaatta atgacccgta cgggattcga
      781 acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa
      841 acaaaatatt gaaacggaga aggagggatt tgaaccctcg cgccggtttc ccgacctaca
      901 cccttagcag gggcgcctct tcagccactt gagtacttcc ccaaaaccaa taagattttg
      961 tactaatggg cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta
     1021 accagctgag ctataggccc ataaaaaact aaaagcgggt gacgagaatc gaactcgcga
     1081 caacagcttg gaaggctgtg gttttaccac taaactacac ccgcatggcg gttccgacgg
     1141 gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac
     1201 cagtgttttt agtttttaat tgcgggagca ggatttgaac ctacgacctt cgggttatga
     1261 gcccgacgag ctaccagact gctccatccc gcgataatat gaaaaaggag gataagggat
     1321 tcgaaccctt gcacggtttt acccgcctga cggttttcaa gaccgttccc ttcagccgga
     1381 cttgggtaat cctccatatg aaacttacaa cgccacaatg gaccttgtag gactcgaacc
     1441 tacgaccgga cggttatgag ccgtctgctc taaccaactg agctaaaggt ccaggctctt
     1501 aaaaatcgcg gcggagggga tcgaaccccc gacctcccgg gtatgaaccg gacgctctag
     1561 ccagctgagc tacaccgcga aaaagatatt cattatttaa atgaaatgga gcctagcggg
     1621 atcgaaccgc tgacctcctg cgtgcaaagc aggcgctctc ccagctgagc taaggccccg
     1681 tgtttaaatt tataattatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa
     1741 ccaagtgctc taccaagctg agctacttcc cgtttaatta acgcgcccaa gaggagtcga
     1801 acccctaacc ttttgatccg tagtcaaaca ctctatccag ttgagctatg ggcgcatatt
     1861 tgaaatgccg aggaccggaa tcgaaccggt acggtgatca ctcaccgcag gattttaagt
     1921 cctgtgcgtc tgccagttcc gccaccccgg cgcgattgct ttggcaaagc ggaaaacggg
     1981 gttcgaaccc gcgaccccca ccttggcaag gtggtgctct accactgagc tatttccgcg
     2041 tgtttaaaga tgccggctaa aggacttgaa ccctcgaccc tctgattaca aatcagatgc
     2101 tctaccaact gagctaagcc ggcatgtaaa acttaaatta tgcgggtgaa gggacttgaa
     2161 cccccacgcc gtaaggcgct agatcctaaa tctagtgcgt ctgccaattc cgccacaccc
     2221 gcaaaaatat gagccgtaca gggctcgaac ctgtgaccct ctgattaaaa gtcagatgct
     2281 ctaccaactg agcta
//
LOCUS       DACCWV010000223         2280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCWV010000223 DACCWV010000000
VERSION     DACCWV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.230"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..94
                     /locus_tag="IVW84_002860"
     CDS             <1..94
                     /locus_tag="IVW84_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7918984.1"
                     /translation="KYGITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            142..612
                     /locus_tag="IVW84_002861"
     CDS             142..612
                     /locus_tag="IVW84_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7918985.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            697..1305
                     /locus_tag="IVW84_002862"
     CDS             697..1305
                     /locus_tag="IVW84_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7918986.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1439..1639
                     /locus_tag="IVW84_002863"
     CDS             1439..1639
                     /locus_tag="IVW84_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7918987.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT          775 a          321 c          457 g          727 t
ORIGIN      
        1 aaaatatggt attacacatg tgacgatcca agcagagaca aaggcaaagg atcaaaaaaa
       61 attaattttt ttaaataaag atgaaagtta ttgaaatata gagtatgata aataagaatt
      121 tttaataagg taggttggct catgaagcag tatcgacagt taaaaggata tttagaagca
      181 aaaggatatt gggagatact agtctatctc ttttttggtg gtttggctac gattgtaaat
      241 tttgttagtt ttgctctcgc acgccaatat tttgatttaa gtatggcctt atccaattct
      301 atttcgtggt tttgttctgt attgtttgct tttgtaacga acaaactttg ggtattccat
      361 tcaaaatcac cgaattttac acatgcactt attgaatgcg gtaagttctt cttctaccgt
      421 atcctttctt acggattgga catgggggca atggtattgt tgatcaatgt tatgaacagc
      481 aatgagtatg tggcgaaaat catcactcaa attattgtta tccttgcgaa ctatattttc
      541 agcaagttgt ttatttttaa agaaactgag gtttttgaag aagaagtagg tcaaagtgaa
      601 aaagacgact aggcataaca attgaagaat ttccttctct ttgttactct ttgtaatgta
      661 gacattatta agaaaacatt gaggaggaat ttttttatga cttacacatt accagatcta
      721 ccttatgcat atgacgcatt ggaaccttat attgacgaag aaacgatgca tctgcatcat
      781 gataagcatc acaatactta tgtgacgaat ttaaattcag caattgagaa atacccagaa
      841 ttaggcgaaa aaacaataga agaattatta tctgatatgg acgctattcc aacagatatc
      901 aagacagctg tacgtaacaa tggtggcgga catgctaacc attcattttt ctgggaaatc
      961 atggcaccaa atgctggtgg cgaacctaca ggagaaataa aagaagcgat taatgaagct
     1021 tttggtgatt tttcttcttt taaagaagaa ttcaaaaaag cagccgctgg acgatttggt
     1081 tctggatggg cttggcttgt aatggaaaat ggaaaattag ctattacctc tactgcaaat
     1141 caagattctc cattgatgga aggaaagaca ccaattctag gtttggacgt ttgggaacat
     1201 gcttattatt tgaaatataa aaatgtccga ccagattata ttgcagcatt ctggaatgtt
     1261 attaattggg atgaagtaaa caaacgtttc gaagcagcaa aataaccatt tcctccgtca
     1321 tatcagtctt aaaaataatt tggaaaataa aagagcgaaa gcttgctagc gttttctaaa
     1381 ttgggtataa tgttcttgca aggttacttg caagaacttt tttggaggat atacatttat
     1441 ggaacacgga acggttaaat ggttcaacaa tgaaaaagga tttgggttta ttacagtaga
     1501 cggcggagat gacgtatttg ttcatttctc agcaattcaa ggagacggct tcaagagcct
     1561 agaagaaggt caagaagtag aattcacgat tgttgaaggt gcacgcggac ctcaagctgc
     1621 tgaagtgact aaattataat aatttgttga ataaagacac gacttcttag agagtcgtgt
     1681 ctttttgtct atttatgaag tttttagatt ttttggctaa gtaaatgcat ttgttcactt
     1741 gacatgtcct aactaattat caagcaagga acattttctt cgttagtaat ggattttaaa
     1801 gaaatttcag tttttggatg ttgaataaaa gaccatttgt gaaaccacta gtatacatac
     1861 tcattaccat gtcaattcta ttactttaaa agaaaatcgt tttactttga agaggagcat
     1921 atctatccat taaaagatga tggtaatgca atcactagtt cgagtcattt taagagccat
     1981 gcagtgagct ggaaataaaa cgaatagaag atgggtttaa caaaaggacc agtcatagga
     2041 agaaattgtc gtgaaatcga atgataatct gaacggtagg caaagactgt taaaaagatg
     2101 ggaaaggtat gtgcgatcat tttactgcac gattatttgg aaaatacctt gttgttcgct
     2161 ttttctggaa aaaatacata aaaaaagata aaacgattgc gaactgcgtg tattcggtct
     2221 ttcgatcttt ttcacggtat ggtatgatag ttaagaattt tgagaaattt taaaaaataa
//
LOCUS       DACCWV010000224         2270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCWV010000224 DACCWV010000000
VERSION     DACCWV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.231"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            191..445
                     /locus_tag="IVW84_002864"
     CDS             191..445
                     /locus_tag="IVW84_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7918988.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IVW84_002865"
     CDS             446..763
                     /locus_tag="IVW84_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7918989.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="IVW84_002866"
     CDS             1322..2257
                     /locus_tag="IVW84_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918990.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DACCWV010000225         2257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCWV010000225 DACCWV010000000
VERSION     DACCWV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.232"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(151..2148)
                     /locus_tag="IVW84_002867"
     CDS             complement(151..2148)
                     /locus_tag="IVW84_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7918991.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          504 c          411 g          768 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttt
//
LOCUS       DACCWV010000226         2227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCWV010000226 DACCWV010000000
VERSION     DACCWV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.233"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(114..638)
                     /locus_tag="IVW84_002868"
     CDS             complement(114..638)
                     /locus_tag="IVW84_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7918992.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(631..1104)
                     /gene="tsaE"
                     /locus_tag="IVW84_002869"
     CDS             complement(631..1104)
                     /gene="tsaE"
                     /locus_tag="IVW84_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7918993.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1227..2207)
                     /gene="pta"
                     /locus_tag="IVW84_002870"
     CDS             complement(1227..2207)
                     /gene="pta"
                     /locus_tag="IVW84_002870"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7918994.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
BASE COUNT          570 a          540 c          366 g          751 t
ORIGIN      
        1 aacaccgttt attcaaaaaa aatggactgc gacacgactt ttgtcacagt cccttcgtgt
       61 gtttttctgc cttctcaaaa ggatgacagt ttctcactct tttcctattt cttctattca
      121 atcaaataac tcattttata tacatctaaa aattcaccta gatctgttct tgcccctcta
      181 ggcatgaccg cttcgatttg gaagcccatt tttcggtaaa ggtgtacggc tctttcatta
      241 cgaacttgaa cgtcaagctc caatcgaaaa agtacaccca tttccttcgc ccaactgatg
      301 atttcttcta gcataagagt gcctagtccc attccccaat attcctgcag aatactgata
      361 ccgacttctc ccacgtgaga caaacgaaat tgactgtctg ccttgacaga tgctgttcca
      421 ataatcgtcc cttcatagat tgcaagcagc aataaattat tattgctttc gtatatataa
      481 tccaactctt cttctaaggt ttctggtggt aacaggagtt ctgcttcatc caaaactaaa
      541 aattctgtct ccttattaac ttgtcccatc atcaaaagaa tgtcttttgc atctcctggc
      601 actgcttccc gcaagatgat cccaacttct tcactcatga ttttcagccc atttttctag
      661 aattcgctgt ttgaaggttt cactttgctc cccgtatgag attagtttca cataccgctt
      721 ttctaaatct gtatcacttt tttctaaaat cagttccaaa taatcctccg gcagtgcttc
      781 ttctaataga tttccccatt caacaacgga aagcccatca ccttcaaaat attcatctaa
      841 ccctaaatct gctccgctag ctgctacccg ataaatatcc atatgataca acggaatccg
      901 accttgacta tattctctaa taatcgtgta tgtcggactt ttgatcattt gcgttatccc
      961 taatccttgg gcaattcctt tcgtcaacgt tgttttaccc gcacctagat caccagtcaa
     1021 tatcagattg tcacctggca ttgcggaatg accaattgcc tctgcgaatt gttctgtcat
     1081 tgttaatcct gatagttcga tcatttttca tcttctcccg ctcttctttc tttttgccag
     1141 tatatcgcgt aatgaagttt ttttaaagaa aaaatgaact agtcaagaac agcagctgct
     1201 gtttttggct agttcgcgtg tatttgttaa tcaatcaatg tttgtgctgc tgtgataatc
     1261 gataatttat atacatcttc ttcatttgct ccacgagaca aatcagaaac cggtttgttc
     1321 aagccttgta gaattggtcc gattgcttca aagttaccga aacgctgagc aattttatag
     1381 ccgatatttc ctgactgcaa ttctggaaat acaaagactg ttgcttgacc agctacttct
     1441 gaatctggcg ctttcagttg tgcaactgaa ggaacataag aagcatcaaa ttgcaattcg
     1501 ccgtcgatca tgtattgtgg cgctagtttt ttagcgattt tagttgcttc aactactttg
     1561 tctacttctg gcgatttcgc tgagcctttt gtcgagaagc tcatcattgc tacttttggt
     1621 tcgatatcga acaattctgc tgtttttgca gaatctacgg cgatttcagc caattcttgc
     1681 gcagttggat tcacgttgat cgcacaatcc gaaaagacat atttttcttg atctcttcca
     1741 cgtaccatga tcatcgctcc gcttgtccga ctgatgccag gctttgtttt gatgatctga
     1801 agtgctgggc gaacagtgtc gccagtcgaa tggatcgctc cagagaccat tccgtctgca
     1861 atccccatat acgtaagcat cgtaccaaag taattcacat cttttaaaat cttttctgct
     1921 tgttctttgg atgcctttcc atttctacgt tcaacaaaag tatccaccat ttcatcccac
     1981 ttatcgtaat tatcagggct gatgatcgta aagctagatg ttttgattcc tctggcatgc
     2041 gctgcatttc ttatttcttc ttcgttccct actaaaatgg gttccacaag ttcttctgct
     2101 ttcaaccggg cagctgcgcc taaaatacga gcatcagtag cttccgggaa aacgattttg
     2161 atcccacggc gaacaatttt aaatcttaaa ctatcaaaca attccacaag atgacctcca
     2221 gacgttt
//
LOCUS       DACCWV010000227         2222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCWV010000227 DACCWV010000000
VERSION     DACCWV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.234"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1009
                     /locus_tag="IVW84_002871"
     CDS             <1..1009
                     /locus_tag="IVW84_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7918995.1"
                     /translation="NFYRESKGDLFIYSEFENARRYSERIKELGLFNDVVFVNHRKIG
                     SSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTKILFSNVYKKNPDVKLNYIEDG
                     LGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKID
                     ENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETL
                     IKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTIN
                     SIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGI
                     I"
     gene            996..>2222
                     /locus_tag="IVW84_002872"
     CDS             996..>2222
                     /locus_tag="IVW84_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7918996.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKK"
BASE COUNT          760 a          278 c          346 g          838 t
ORIGIN      
        1 aaatttttac cgagaatcaa aaggagattt atttatttat tctgaatttg aaaacgctag
       61 aagatatagt gagcgtataa aagaattagg gctatttaat gatgttgttt ttgtgaatca
      121 ccggaaaatc ggttcttcct ttaaacgtaa aattaacttg ttaactaacc ataatcaatt
      181 tttctcggat gatttatccg tttatgatga aatttttata cagggggaaa attatttcac
      241 aaaaatactt ttctccaatg tttacaaaaa aaatccagat gtaaaactaa attatataga
      301 agatggtttg ggtacttatt tgaagaagag tatattttca acaagtaaaa acgttcaaag
      361 agtttttaaa atattaaata aaaatagcat cttcaaaaaa aaaattgacg attattttgt
      421 ctattgtccg gaattgttac tttatgatgt aaaaaatata aaaaaaattc ccaaaataga
      481 tgaaaacagt aaattgatga aaaacataca atcaatcttt gggatagaag aagtttttgt
      541 cggagataaa tgtatctttt ttgatcaacc tttattatct gatggttatg gcatagatga
      601 aacagatatt ttcgctaaag taaatagtat attttctgga aaaaaagaaa ctttaattaa
      661 gcttcatcct agagcaagga caaatcgtta tggtgaaaaa aacatattga attccagttt
      721 accgtttgaa gtcttgctac ttgaaaatga cttctcttca acacttttga ttagtcctat
      781 atctacaatt tctttttcac catctatgat gtttgaaaag acgatcaact ctattatgtt
      841 ggctaaatta attttaaatg aatatacagc attatctgaa gataaaaaag aaacattgac
      901 aaatattgtt cttttctgtg aacattataa ccgtctatca gaagaaaaaa ttttactccc
      961 agaaaactgg gcagaattaa gaaagttggt agagtatggg attatttaac acactttcca
     1021 agtcaaaatt agttaagtcc agtttctggt atactatagg gaattttttt attaagggaa
     1081 tttcattcat aactattcct atttttgtaa atattactac tcaagcagaa tatggtttga
     1141 ttaataattt cacttcattt gtatcaattt tttcgattct tgttgggcta agcctaaatt
     1201 cggcaattaa taatgcaagt tttgactata aagatgacat aaatgaattt atgtcatcgg
     1261 tactttctct agctacactt tcgttaattg tgttcttatt aatagggaac atatattttt
     1321 tgttttcaaa tgcttttttt gatatgaacc aacttgtatt taatttgatg ttattacaaa
     1381 gttttgctag ttttgtaatt agttatatcc agtcttattt tacgattaac tttaaccatt
     1441 ttaaatattt gattttatct tttatatcta cttttttgaa tattgcaatc tctgtagctt
     1501 tgatgcagac attatatttt gatgataaat atgttggcag agtagtcggc ggaacactgt
     1561 catttgtgtt gataggttta gtaatttatt taagaataat gataaagggt gctcattttt
     1621 ttgataaaaa acagtggaga tacgctttaa aaatctcttt gccgctaatc ccacattcct
     1681 tagcaaatat agttttagca caattcgata gaattatgat taattcttat ttaggtcctg
     1741 ctgcgggggg aatatattcc tacattagta acataggtat tattctaagc gtaatatgga
     1801 tgtctactaa taacgcctgg gttccatggt tctatggtga gatgagtaaa aataatgaaa
     1861 aaaaagtgaa gaaagttagt aactattatt tagtagtctt tactttgatt acgatgattg
     1921 taatggtagt ttcgattgat ttagcaaaat ttatggctcc gaaagactat tattctgggt
     1981 tatctttagt ggttccgata accttaggct atttctttca gtttttatat agtttaccag
     2041 ttaatgctga attttatgag aaaaaaacat cgtttattgc gattggtaca gtagcaagtg
     2101 ctatagttaa tatactatta aacatgattt tcatacctag gttaggaata attgctgctg
     2161 gatacacaac cgtatttacg tattttttac tttttatttt tcattataat atcgctaaaa
     2221 aa
//
LOCUS       DACCWV010000228         2219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCWV010000228 DACCWV010000000
VERSION     DACCWV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.235"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            36..476
                     /locus_tag="IVW84_002873"
     CDS             36..476
                     /locus_tag="IVW84_002873"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7918997.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            604..1287
                     /locus_tag="IVW84_002874"
     CDS             604..1287
                     /locus_tag="IVW84_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7918998.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1596..1669
                     /locus_tag="IVW84_002875"
     tRNA            1596..1669
                     /locus_tag="IVW84_002875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1630..1632,aa:Arg,seq:tct)
     gene            1828..2187
                     /locus_tag="IVW84_002876"
     CDS             1828..2187
                     /locus_tag="IVW84_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7918999.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          737 a          345 c          451 g          686 t
ORIGIN      
        1 tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga
       61 ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat
      121 atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa
      181 attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt
      241 tttatacaaa agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata
      301 ttatcggtag tcccatcttt cagattccat atattggact agtcctttca aaaacggcat
      361 ttgcatggat tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat
      421 ggaatacctt tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat
      481 gttaaaaaca taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa
      541 aacactcatg cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt
      601 agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg
      661 cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta
      721 gcagaagtac cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag
      781 cttcagcctg aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg
      841 gccacgacaa tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc
      901 gagaaagcta aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa
      961 ggtcaagcag cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat
     1021 agtgcaaaat caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt
     1081 ggcgttttga tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag
     1141 attccccgtg gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg
     1201 gatcatcccg atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca
     1261 aaggaatcag aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt
     1321 gaatcgaggg ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag
     1381 gatacaggaa atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat
     1441 tgataacctt agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg
     1501 ggccaaaata attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt
     1561 aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag
     1621 catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg
     1681 ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc
     1741 aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata
     1801 aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta
     1861 gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg
     1921 acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg
     1981 tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat
     2041 ctttcttccg catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca
     2101 ttatatggca gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg
     2161 ttggcattgc tttctatttt cttataaata tagaaaaaat aagaatttat ctaacagga
//
LOCUS       DACCWV010000229         2135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCWV010000229 DACCWV010000000
VERSION     DACCWV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.236"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..624
                     /locus_tag="IVW84_002877"
     CDS             <1..624
                     /locus_tag="IVW84_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7919000.1"
                     /translation="LYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKYKAFRD
                     FERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAY
                     LKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRL
                     SKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            659..1426
                     /locus_tag="IVW84_002878"
     CDS             659..1426
                     /locus_tag="IVW84_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7919001.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            1616..2014
                     /locus_tag="IVW84_002879"
     CDS             1616..2014
                     /locus_tag="IVW84_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7919002.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          804 a          290 c          384 g          657 t
ORIGIN      
        1 ctgtatgaag caaatagaga taacttaaca tggagtacaa aatcacttga tagattaaaa
       61 aatcctgttt tctccattgc agatttacga aagaaattta gtattgagaa aaaatataaa
      121 gcttttcgag actttgaaag acgtgtaatt aaagaaccga ttgcccaact gaataaatac
      181 actgatttta aaattgacta tgagcttatc aagaagggtc atacttatac aaacataaag
      241 ttcttgctta cacgtaccat gtcagttccg gtaccttata agcaagaaca aagagaccct
      301 gcttatttaa aaggcgcaga agataaaaca gagaaaatga atcaatcgta cttgaaagct
      361 atgaatagca aatacactaa attactaatc aaaaactttt tgatcagttc tgccgactta
      421 tgcgatctta aacttatgag ccgacttcag aataaagttt atccgcttta tgatcgttta
      481 tccaaagata gaggatttga atcagttgtc aatcacctat cttacgttgc tactcataag
      541 gaggattaca caataaaaaa tactgttaaa tatctcgaaa aatcaattac gagttatttg
      601 ccttatgtga acctttatga ttaacaactg aaaaaatgcg tgaaggaagg aaaaactttt
      661 gaaaaaagat gaactgacaa agtacaatga ggagttctta gcaacaattg atatgaaaat
      721 ggatttaaag tcgtatgaac tttatcaatt gctgttcagt tatatctcac ccacaaagct
      781 aaagaaaaca ggcgtagtag atttacctgt taaagcgatt attaattaca tggatgttga
      841 tcaaaatctt gaaacggaac taaaaaccat agttaacact ttgcaaagac aagcagttta
      901 tgaaatatcg aatctagaca agaaatataa gacgtatact actgtatctg ctattgatcg
      961 gattcattgg gatttagaag agcatagtgt atctttcagt ttttcagaag atgtaattcc
     1021 cttaattgtc tcaatcaaaa agaagtatcc gttctttgtt tttaaagact tagcatcttt
     1081 tcaaaaaaaa tatagcatcg tgttatacaa atatttggcg atgtattata aaaaatatct
     1141 tatcgccaaa aataaaggag attttgagaa agctgaaata tataagaatc ccctgattga
     1201 aatagttaaa attcgtgaga ttacgaaaac agaaaatgag tataaaacaa tatcgaattt
     1261 tgatagacgt gttttaaggg aatcagttga agaaattaat gaaaaatcaa tgctgaagat
     1321 tgattttgct aaagttagaa aaggcagaaa ttacaatttt gctgtccagt ttaatatttc
     1381 tagtgagttt gatgaaaaac cttatagaga aaaaaaatta ggttaatgca aaatatatct
     1441 tactcgtttg aagatgcttc agacgtatgt taagatatgt agtgccttgg gagcaaggta
     1501 ttaatctcct ttagaggtta attagcttgt gatcgcctat caattttttg gtaggcgatt
     1561 ctttttttga aaccaaaaag caaaaattat tttttaatta gagaggtgca atgcgatgaa
     1621 atactttttt tgtgcagtat tcgaatggaa aggaaatgaa tatgcagtta aattcccaga
     1681 ttttccagac ttatatacgc atggtgaaac tttagaagat gcttttgaga tggcgcaaga
     1741 agtattggaa ggatacttgg tgcttttaga gttgcaagaa aaagaaattc catcgcctac
     1801 gcctgttcaa gagttaaatg caagaatcaa gcgatctgag aatgttaatt tatttgtcgt
     1861 aaaagctaat acgaaagttg ctaaagctcg aaacaagcaa aattatgatc gaaaatcagt
     1921 gactgtggaa cactatttaa atgttttagc ggctgaagag aatattaatc tgtcgaaagc
     1981 tttgaatgat ttgttgagag aaaagttgat ttgatgtctc tcagatgatc atattaagca
     2041 aaaacagctt gttttaggac gtatggattt aagaaagaca aattatcaaa gaatatatta
     2101 aaagctctca gaagcgttct gagagctttt aaatt
//
LOCUS       DACCWV010000230         2069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCWV010000230 DACCWV010000000
VERSION     DACCWV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.237"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(555..920)
                     /locus_tag="IVW84_002880"
     CDS             complement(555..920)
                     /locus_tag="IVW84_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919003.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(996..1241)
                     /locus_tag="IVW84_002881"
     CDS             complement(996..1241)
                     /locus_tag="IVW84_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7919004.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1311..1565)
                     /locus_tag="IVW84_002882"
     CDS             complement(1311..1565)
                     /locus_tag="IVW84_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919005.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1729..2052)
                     /locus_tag="IVW84_002883"
     CDS             complement(1729..2052)
                     /locus_tag="IVW84_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919006.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          769 a          357 c          369 g          574 t
ORIGIN      
        1 gtctgaaaac aaaaaaagaa agcgttatta ttttagtcta ttttcagggg gggaacctta
       61 tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact
      121 ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat
      181 gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca
      241 gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa
      301 tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg
      361 cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac
      421 caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg
      481 acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg
      541 cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct
      601 gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa
      661 tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg
      721 ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa
      781 ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt
      841 aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat
      901 ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg
      961 ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct
     1021 gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca
     1081 atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt
     1141 aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga
     1201 caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata
     1261 gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt
     1321 ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat
     1381 tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag
     1441 tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga
     1501 taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta
     1561 atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat
     1621 cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga
     1681 acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa
     1741 aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac
     1801 taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt
     1861 ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt
     1921 aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg
     1981 gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat
     2041 cactcctaac attgagaata aataaaaga
//
LOCUS       DACCWV010000231         2056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCWV010000231 DACCWV010000000
VERSION     DACCWV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.238"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            493..714
                     /locus_tag="IVW84_002884"
     CDS             493..714
                     /locus_tag="IVW84_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919007.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IVW84_002885"
     CDS             715..972
                     /locus_tag="IVW84_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919008.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IVW84_002886"
     CDS             complement(1108..1710)
                     /locus_tag="IVW84_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7919009.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IVW84_002887"
     CDS             complement(1777..1932)
                     /locus_tag="IVW84_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7919010.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACCWV010000232         2054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCWV010000232 DACCWV010000000
VERSION     DACCWV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.239"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..590)
                     /locus_tag="IVW84_002888"
     CDS             complement(27..590)
                     /locus_tag="IVW84_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7919011.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(645..824)
                     /locus_tag="IVW84_002889"
     CDS             complement(645..824)
                     /locus_tag="IVW84_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919012.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(901..1410)
                     /locus_tag="IVW84_002890"
     CDS             complement(901..1410)
                     /locus_tag="IVW84_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919013.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(1506..1886)
                     /locus_tag="IVW84_002891"
     CDS             complement(1506..1886)
                     /locus_tag="IVW84_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7919014.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT          568 a          423 c          326 g          737 t
ORIGIN      
        1 gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc ataaagatga
       61 ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa ttttttagcc
      121 tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt cttcgccaat
      181 ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc ttctgataca
      241 ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa caaacgtgta
      301 gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat gattgattct
      361 tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc ttctacgata
      421 cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa aatctctaaa
      481 gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag aatcactaag
      541 atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat agtttcctct
      601 ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt taaaaaatct
      661 tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc agttcggcaa
      721 taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt gacttcttcg
      781 tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc tcaacttcct
      841 ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa tatccttctt
      901 ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga ctttataata
      961 aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt tcccgtagta
     1021 gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt tttctatatc
     1081 aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc cagtggatgg
     1141 attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg cattcgtagg
     1201 attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt cttttgtttc
     1261 gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt cactaatatc
     1321 atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta ttacgatgag
     1381 ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt ttttttaaag
     1441 aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg acaattttgt
     1501 tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa cgtcattttt
     1561 tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga ctgcaattct
     1621 gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc acaagcttct
     1681 ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc agcacttcct
     1741 agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct gattacttca
     1801 aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac aatgatggct
     1861 atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta agctcggatc
     1921 tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc tgtttttcct
     1981 ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac tcttcctgtt
     2041 cccaatataa taaa
//
LOCUS       DACCWV010000233         1997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCWV010000233 DACCWV010000000
VERSION     DACCWV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.240"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..372
                     /locus_tag="IVW84_002892"
     CDS             <1..372
                     /locus_tag="IVW84_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7919015.1"
                     /translation="VSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRF
                     PEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDW
                     QKVMAAEVIATYLDGDLVYKK"
     gene            420..1820
                     /gene="yfcC"
                     /locus_tag="IVW84_002893"
     CDS             420..1820
                     /gene="yfcC"
                     /locus_tag="IVW84_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7919016.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1853..>1997
                     /locus_tag="IVW84_002894"
     CDS             1853..>1997
                     /locus_tag="IVW84_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7919017.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          550 a          339 c          432 g          676 t
ORIGIN      
        1 gtttccacag aggacttctt taattattgt cgattagagg aaagtggtgt aacagtcact
       61 agcggtacag atttaccgct gtttattact gatattcctg aatcgattat aagagcagta
      121 ttccgtcgtt ttcctgaagg agaagaagtc tgggagaaag aaacaagctt cacgacaacc
      181 gagctgctga aaagttttac aagcaacagc ttcaaagtaa atggtttatc ggagtacgga
      241 gaaataaaag tagggaaaag cgccagctta gctgttttca gcgaggacct ctgtactgat
      301 gactggcaaa aagtgatggc tgcggaagtt atcgctacct atcttgatgg tgacttagtt
      361 tacaagaaat aatgcttttc gatataacgt tataaaacga attaaagaga ggttaaaaaa
      421 tggaaacaat gaaaagaaaa ttcaccttcc cacatagtta tacattgatt tttatcttaa
      481 ttattttggc tgcaatttta acttggataa ttcctagtgg tcagtttgag acagttgttg
      541 aaaacgtcgc aggtatggaa aaaacaacgg ttgttccggg aacctatcac caaatcgata
      601 aggcagatta tggacaaggt ttttcctcta tactaatggc accagctgaa gggattattt
      661 cagctgttga ggttgttgct tttgttctca tcgttggtgg tgccttcggg attatcctaa
      721 aaacgggagc catcgatcga gggctgttta ccttagcaaa agttctggga gacaagggga
      781 tcttagttat tccgatgtcg atgattctat tcagcttagg aggatcaacc tttggcatga
      841 gcgaggaagt cattccctta tttgcggtgt ttatttctct gatgttttct cttggctatg
      901 attccatgac tgcgatcttg attctatttt taagtacaca agcgggatat gttgggtcga
      961 cgattaatcc cttcaacgtt ttaatcgcac agggagtagc tggcgtttac ggaaatcccc
     1021 tgctttggtt tcggatgcta tcctggattg tcattactgg gatttcaatt gttttcacca
     1081 tgctttatgc tagcagagtc aaaaagaatc cactgtcctc tattgtttat gagagtgatt
     1141 taaaggttaa gcataagttt caaaattttg acactgaaaa agagttttca acacaagata
     1201 aactaattat cgccggtttt atcgtaggtt taggaatcat gatttggggt atcatttcca
     1261 agggatggta tatggttgaa atttctgcac tattcttagg attaggtctt tttgctgggg
     1321 gcatcgctag attttctcaa aaggaaattg ctgaagcttt tgtcgaaggc tgttcagatt
     1381 tcgcctatgc tgctgtaatc attgggctag ctagaggagt cttggtaatc ttggaaggtg
     1441 gaatgattat tgacactatt ctaaacagct tggctaacct tttaggtggt cttccaaaat
     1501 tcctgttcac tacgattcta ctcatttctc aagtaattgt ttcctttttt gttccatcct
     1561 cctcaggagc tgcggcatta acgatgccgg ttatggcacc attgggtgat ctaatgaatg
     1621 taagtcgtga tgttgttgtt ctaagtaacc agtttggaaa tggtttaatg aatattatct
     1681 ccccaactag tggtgtatta ctggcaggat tagccatcgc agagattaat ttctctaaat
     1741 ggtttaaggt tggcttcaaa atatttgcca tcttaaccct tgtttctgct gttttgttat
     1801 acattgctac actcttttag tcttaatcat ccattaatag gaggcaatag ttatgattaa
     1861 aataaatcaa ttagcaggaa tgaatatcca ctatttattt tattctctca aagaattttt
     1921 cgcttatcaa cagaaaattg gtatttcttc tgttgaactt tggggaggtg ctccgcattt
     1981 tttcatagat gctttta
//
LOCUS       DACCWV010000234         1985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCWV010000234 DACCWV010000000
VERSION     DACCWV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.241"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..708
                     /locus_tag="IVW84_002895"
     CDS             <1..708
                     /locus_tag="IVW84_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7919018.1"
                     /translation="FDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHD
                     LFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQ
                     VRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYLPG
                     DVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESAND
                     LKIELDTWLLGLDVTEK"
     gene            908..1111
                     /locus_tag="IVW84_002896"
     CDS             908..1111
                     /locus_tag="IVW84_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919019.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1671..>1985
                     /locus_tag="IVW84_002897"
     CDS             1671..>1985
                     /locus_tag="IVW84_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7919020.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFD"
BASE COUNT          658 a          306 c          462 g          559 t
ORIGIN      
        1 tttgaccaat atggagaaga tatcgcctat atcgaaatgt taaaatttca ccctgacaaa
       61 ggtcgtattg attttaatcc aaataaaatt ggcgagtttt taaaggggtc gatgaaacaa
      121 ttcattcatg atttgtttct ggatgcgcat ttctcacgtg cagatattgc ttgcgatatt
      181 ctcggtgttc ctgatgattt tattacccag tatcgaatcg ttgaccctgt gtcttttaag
      241 ccaatttatg gccgttctgg caaattagaa acagcgtact ggggtagtcg tgccagcgag
      301 cgacaagtgc ggatgtacaa caaaaagctt gaacaggaac gaaaaaaaca gattgtacca
      361 aaagaaatcg aaacatggtg gcgtatagaa atgcagctaa gacgtggcaa agcaacagat
      421 tggcatgcga tggttcggga aagtttagat agctttgcaa gtcctcatta cttgcctgga
      481 gatgtaaaac ctgttgatag aattatgata aaagggctga atcaagatca ttcagagtgg
      541 gcgtatatta gcagaaattt gaaatataga ttgcgcaaat tgttgaaaga agaaagccaa
      601 aacgatgagt tgacgaatca tttacgagag acgtttaagg aatctgcaaa cgacttgaaa
      661 atagaattag acacgtggct tttgggtttg gacgtgacag agaagtgata ccgaataaga
      721 aaagtcagat gagtgagagt aaatttacgt caatgtttaa tgacgtgagt ttacgataag
      781 cgattatttt gcgtgaagca aaataaagcg taaaaggagt tgagagcgtg acacgctttc
      841 ggctcctttt ttcgttgtca cttgtgacag cgaataccct cggagagcat ttggaattgt
      901 gacagccatg tcacaattag gaaaatgtta ctctttgtca aagagtaaca gaaaggttta
      961 cactcttttt attagcgaca gcgcgaagga ggggtttaac ttggggttta gtatttcgtt
     1021 caaaaaggga acaaagaata caagcatttc tcataataat cgtgagttaa ctgatagcca
     1081 aaaaatcaat ggtttgtctg gtagaaaata attagcagat ttacaagtgg gaaataaaaa
     1141 gctgaaagaa gaaaattgca tttcaaacta cattttctac aatagctcta aaatggcata
     1201 ttttgcgttc tgagcggttt tagcgtttat ttcgtttagt tatcggcaca atcgttaaaa
     1261 cacgttagag gagcgaaaat ggagcctgta aggcgactgg tagcggaacg atcgtgttag
     1321 attgtgaaaa ccgatagctg aatgaaataa acagcgggtg cgccctttcc atttcggcag
     1381 gaggaggctc aaggggttcg gggaatgaaa ttccctgagg tctttttatt tttttaggag
     1441 gagtgctata atcaaagaaa acgagaaaaa agagcaaaat aaaagccgag cagtgcgcta
     1501 acactctcga ctatttacgg aaactacttc acctagctcc gtaactcagg ttcattatac
     1561 gaatattttt aggtttcttc aagctagaga tttttggata tgaattttta gggttacgtg
     1621 aactctggaa attctttcca aaagtctctt ttttcgtctg gaggacgaaa atggcaaatg
     1681 aaaatcagtt gagtgaaatt caatcaaaaa ttgaaggtcg aggggcagaa aaaagaacaa
     1741 cgataaatca gctcgaaaaa cttaaccgta tttctaacag gcggttaagt gttgacgata
     1801 aggtacaccc cctaaaatta agttggtcaa tcgatcgaat tacaatcgtc ggaaaactga
     1861 atgataccat agcttattgg acgcaaaatg gagttgtgtt tgtcgatttt gagcaattaa
     1921 tgcgtttgaa tgaaaatcaa gggtatttga agtcggttgg tacaaatggt tggcaattat
     1981 ttgac
//
LOCUS       DACCWV010000235         1966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCWV010000235 DACCWV010000000
VERSION     DACCWV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.242"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            270..836
                     /gene="amaP"
                     /locus_tag="IVW84_002898"
     CDS             270..836
                     /gene="amaP"
                     /locus_tag="IVW84_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7919021.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            849..1037
                     /locus_tag="IVW84_002899"
     CDS             849..1037
                     /locus_tag="IVW84_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7919022.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1050..1607
                     /locus_tag="IVW84_002900"
     CDS             1050..1607
                     /locus_tag="IVW84_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7919023.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1639..1881
                     /locus_tag="IVW84_002901"
     CDS             1639..1881
                     /locus_tag="IVW84_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7919024.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          305 c          404 g          541 t
ORIGIN      
        1 aaatagaatc ttgtaataat aactgagcat aatctttgca atttttaatc ttgaataata
       61 tcctttaagt atagccagca atcaaaagta aatagagaat aagtaagcaa ttgttatttc
      121 agtatcagac tggcaataaa acgccgttat gatccagcaa tgtttggtag cgctttattt
      181 actgatgcta tgctaactgt tcgctatcta tactgtgatt gtcggtagag ttatttacag
      241 ggtaaaaata agtttatagg aggtggaaaa tgaataaggg gttaaaaata atcggtgtta
      301 tcatgagttt actgctactt tcagtatttc tagcgattgc gttgatcaat gcgccgtata
      361 ttatgcctga acaattggaa cgatttcgct tctttacttt aacgaattac tacatgcaac
      421 aatatatttt ctgggcagct gtagtatttg ccgttcttgc tattatcatc ttgctagtcg
      481 ttcttttcta tccaaagtca agaggaactt tcgttatgaa acgggaagat ggcaaactaa
      541 caatcgacaa aaaagcgatc gaagggttgg tacgttccca tttacacgaa gaagagttca
      601 ttcattcacc taaagtacgg atacgttcaa cgaaaaatcg aatccatatc catgtcaacg
      661 gagacttgaa acgaacttct tcattagtag ggaaaacagg tgtattgatg caagatatcg
      721 aagaagaagt aactaaagta ctcggaacaa aagaaacggt caaagtggca gtcacataca
      781 gcggttatga agaacaagaa gatacaagag actacaagca ctctcgagtg gaataggagg
      841 gccaaataat gaaagaacta ttttcagcat acaagctccc tattatcttt gggggtcttg
      901 gactactgct agctgtttta tggttaacga ttggattttt caagacattg ttgatcataa
      961 tcatgacagc aattggcgca ggaatcggtt tttatttgaa acagtcagga atagttgatc
     1021 aattttttaa atcataggag gaaacaaaga tggaaaacaa aaacatgaat acaacaaatg
     1081 gatcagtttt ggcaaatgaa aaaggagaaa accgcatgat gaatggtgaa ttgacttatg
     1141 aagacaaagt catccaaaaa atcatcggtt atgcattgaa agatgtagat ggtctattga
     1201 cattagatgg cggatttttc tcaaatatgg ctgaaaaatt agtcaacaca gataacgtaa
     1261 cagcaggtat caatacagaa gtaggtaaga aacaagtagc tgtggatcta gatgtgatcg
     1321 tagaatacgg taaagacatc gaagatattt acaaccaaat caaagaattg atcagtactg
     1381 aagtaaacaa tatgacacat ttagatgtta tcgaagtgaa tgtgaatgtt gcagacatca
     1441 aaacacaaga agaataccaa aaagatcaag aaacagtaca agacaaagta acagaagcag
     1501 caaaatccac aggacaattt gcttcacgcc aaacagataa agcaaaatct gctgtaggca
     1561 aaggtgcaca aaaagtcaaa gaaaataacg aaccacgtgt acaataagaa gtaagaagtc
     1621 agttaggagg aatttaaaat gggatttatt tgggcattaa tcgtcggcgg tatcattgga
     1681 gcaattgcag gagcaatcac gaataaagga tcatcaatgg ggatcatcgc aaacatcatt
     1741 gctggtttag taggttcagc aattggacaa gctttactag gaacttgggg accaacaatt
     1801 gcaggaatgg ctatcattcc atcattgatc ggtgcaatca ttttagtatt agtcgtttca
     1861 ttctttgcac gtaaattata agcaaattga agaaaaactt atgttgaaat gaagaacaaa
     1921 tttgagaacc gtagcgacac tcgctgcggt tcttttttat atattt
//
LOCUS       DACCWV010000236         1928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCWV010000236 DACCWV010000000
VERSION     DACCWV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.243"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(42..1841)
                     /locus_tag="IVW84_002902"
     CDS             complement(42..1841)
                     /locus_tag="IVW84_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7919025.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          664 a          365 c          348 g          551 t
ORIGIN      
        1 ttttttgttg tataaagact tttttcatta acgatttttg attagccgac tcccaaaaca
       61 aaatttagca gtttatctga ataagtgatt tcttcatctg tttgtttcac actttcattt
      121 agtagtccca tctcatcggt tatgatacca tccacaccat aaaacatcat gcgactcatg
      181 gcatctgaat cgttgattgt ccatacatat acttcttttc cgtcttggtg ggcagaatta
      241 atgaagttac ggttgatcgt actatattcc attgtcagaa aatcagcagg cgtcacaggg
      301 ggcccaacaa tattgaaagg taagatgtag cccacataaa agtttggttc tgcttctttt
      361 aaatctgaaa cggtttggta cgtcaaactt tgcaaaatat gatggttctc taagataact
      421 ttgcggtatt tatggacaaa tcgttcgaca aggtcatccg aatctttttt agaggtcttg
      481 atctcaataa gtaatttttg gtttagctta tgtgcttttt ccaaataaga atcaaaagaa
      541 caaacagggg cttgcactcc gttttcagta actgtaagat ttgtcaattc tttcaatgta
      601 agatcctgtg gtgttccatt tacccctgtt aagtgacgaa gatttgcatc atgcatcact
      661 acgaaatgac catcttttgt ttcctgaaca tccatctcta cataatcagg atgatagtct
      721 ctgcttgttt tttcaagtgc ttctaaagta ttttgcgccc cattttgggt actcacacct
      781 ctgtgagaga cagtcactgg ttttgtctga acagctgaag tcagataatc catattgtaa
      841 agacagacgc caataccgaa aaaaactgca aaaagagtca aaccagtgtt tttcagcgca
      901 gagaaacgca agttgggggc ttgtgggata aaccacttag ggatctcagg caagaagcct
      961 tcgtcatcca tgaaatcaac aatgatataa aaaatcccta cggtagacat cacgatattg
     1021 aataataaaa tcccttgtaa aagcgtcata gcaaacacag cgctgatcaa tgcatagtct
     1081 gggaaaaatt gttcaaccat actttgtgcc aaaataacaa cgatatatcc taaagaagaa
     1141 aggagtaaaa tcgtaccgcc aatcacaata aattggcccg tgatggccaa aagacgcgat
     1201 ttggtcaatg accagctttc ccgaatggct gctcgaaaag aacgatctcg tagaatcatt
     1261 tccggtaaag caaaaatcag tcgaatccca atatagccaa ggaaaatata aacgagtaaa
     1321 aaggaactga cgatgatcca acgattagca aaaataaaat ccataatgaa agcggggatt
     1381 tttatttttg acagtagatc agaattaaaa cttaatccgc tgatcggtag aatcaataaa
     1441 aaataagcaa ggaaaaacaa aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc
     1501 aagtgtaaga gttgcttcaa agagattggt tctttctttt taataaaaaa aacactcatc
     1561 aaaagaaaag taaattcgaa ataaactaac agtaaaatcg tcatcaaaac tacgattaac
     1621 gagagtagaa ccccaggatg ttgagaaaaa attgaaggaa tattgtcata agacaaatag
     1681 tcaattgccc ctcggtgtaa aataaaacgg gtcgtactgc tcaataaggg taaacaaata
     1741 aataaaataa agccatgcat cagcaagaca tcacggaaat aagcttgtgt tccttttaaa
     1801 aagtcccaag cattacctaa gctacgtttc aaataattca tcctatcacc tgctcttcac
     1861 attgtagcgc aaatctaatg aaaaaggaaa gggtgcagtg tgttttgcag accctttccc
     1921 agatttaa
//
LOCUS       DACCWV010000237         1905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCWV010000237 DACCWV010000000
VERSION     DACCWV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.244"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(599..889)
                     /locus_tag="IVW84_002903"
     CDS             complement(599..889)
                     /locus_tag="IVW84_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919026.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1097..1788)
                     /locus_tag="IVW84_002904"
                     /pseudo
     CDS             complement(1097..1788)
                     /locus_tag="IVW84_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          522 a          366 c          291 g          726 t
ORIGIN      
        1 aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta
       61 gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac
      121 aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa
      181 atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt
      241 tacttttacg actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt
      301 ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc
      361 tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc
      421 aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt
      481 agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt
      541 ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct
      601 agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta
      661 ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat
      721 tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac
      781 tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac
      841 taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata accttcacct
      901 caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt
      961 gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac
     1021 atgtactaaa aaaaagaggc agcagtatag attactgttg cttctttact aatttaggtt
     1081 tacttgttaa gaaagattat ttacgcaaat gttcttcaac aatttgctga gatttatcag
     1141 ctgtatcttc cacttcatct tttgcttttt ctaacttttg aatatattgt ttcaagcttg
     1201 agttttcagc agttgcttcg tctaatttct tttgtgtatc tgttaactga tttctacatt
     1261 tgttttttct tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa
     1321 ttctgaaagt tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt
     1381 gatttcatca tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct
     1441 ttgtagttgt gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga
     1501 ttgatttttt tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt
     1561 tgaatcttgc aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg
     1621 ctctttatct gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc
     1681 tgtctctcca ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag
     1741 acatagaaat gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc
     1801 ttaatccaat acttttacat gctaaaagta agtgatctca aacacaatgt caagcgtttt
     1861 taaattacat atgtaatttt aatataaata ctatcaaata ctcat
//
LOCUS       DACCWV010000238         1862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCWV010000238 DACCWV010000000
VERSION     DACCWV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.245"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..735)
                     /locus_tag="IVW84_002905"
     CDS             complement(34..735)
                     /locus_tag="IVW84_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7919027.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="IVW84_002906"
     CDS             complement(751..1191)
                     /locus_tag="IVW84_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7919028.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="IVW84_002907"
     CDS             complement(1191..1766)
                     /locus_tag="IVW84_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7919029.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLISWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          522 a          400 c          289 g          651 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaataa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     1861 gg
//
LOCUS       DACCWV010000239         1854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCWV010000239 DACCWV010000000
VERSION     DACCWV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.246"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVW84_002908"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVW84_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7919030.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IVW84_002909"
     CDS             complement(1305..1709)
                     /locus_tag="IVW84_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7919031.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DACCWV010000240         1789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCWV010000240 DACCWV010000000
VERSION     DACCWV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.247"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            26..508
                     /locus_tag="IVW84_002910"
     CDS             26..508
                     /locus_tag="IVW84_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919032.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            705..1595
                     /gene="pstS"
                     /locus_tag="IVW84_002911"
     CDS             705..1595
                     /gene="pstS"
                     /locus_tag="IVW84_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7919033.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          592 a          308 c          373 g          516 t
ORIGIN      
        1 gaaaaagtag tttgggggaa cgttaatgag aagaaaagca gtaatcgatc ggctttattt
       61 atttttttgg ttttttccta tcgttttgct accttttaca tgggtaaatg ctgttgtaca
      121 aggtgcggag atattaggcc tgatccaagg aagcggattt gtggtttcta ggaattttcc
      181 agctttgatt gttttctatt tagtgataag tgtaggtctc ttattaccaa aatctctgat
      241 caatcactac aattggcagc tgctttactt ttcttgctta ttagtattta catctatgtt
      301 ttctttgctg cctcgcttga ttatcggccc taatggtttg agcgagagtg gattgaagga
      361 tttagcttat tcctccatgt atttcttgac gatcaaacca tggttctatt tagtaattgc
      421 ctcatttatt cttgcaacat ttttttactt ctggacagaa atcaggataa aaagtgaacg
      481 atctgtatct ataaaaaata actattgatg gtggatcatc agtaaaaaaa catatgagct
      541 gcgtaaaatg catgtttctc atatgctttt tttattatta tgtaaagaat gcgaaaaacg
      601 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
      661 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
      721 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
      781 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
      841 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
      901 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
      961 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
     1021 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcacca atcgtcaata
     1081 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
     1141 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
     1201 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
     1261 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
     1321 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
     1381 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
     1441 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
     1501 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
     1561 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     1621 tcgttactat taaggaaaaa tcaactatgt ttccccacat atacattttt cctttaattg
     1681 tgaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
     1741 aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtaact
//
LOCUS       DACCWV010000241         1786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCWV010000241 DACCWV010000000
VERSION     DACCWV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.248"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            207..803
                     /locus_tag="IVW84_002912"
     CDS             207..803
                     /locus_tag="IVW84_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7919034.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(861..1343)
                     /locus_tag="IVW84_002913"
     CDS             complement(861..1343)
                     /locus_tag="IVW84_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919035.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1449..1646)
                     /locus_tag="IVW84_002914"
     CDS             complement(1449..1646)
                     /locus_tag="IVW84_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919036.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          604 a          362 c          271 g          549 t
ORIGIN      
        1 caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa aatccttaca
       61 taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt gagcatatct
      121 gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa aaaatgcaaa
      181 acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg aaaaaccgcc
      241 gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta acaactttga
      301 tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca cgcgcagtta
      361 tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca cttcgcccat
      421 tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc aaaaatggtt
      481 atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa gaacaatttg
      541 aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc gcaacagcta
      601 atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat tacaacccag
      661 ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa ctacgttcgc
      721 aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg aacgatgccg
      781 atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt gtgacaaaca
      841 tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc tgcctcatcg
      901 tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa tttcagcatc
      961 ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc ttttggctga
     1021 tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa gctattcaaa
     1081 acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa aatcattgct
     1141 gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt ataccaaatg
     1201 actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac agtccgataa
     1261 ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa taaaaacaat
     1321 agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc agaaaatagg
     1381 aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg cacgtccaat
     1441 tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt ttcatctaat
     1501 aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt tgtcattttt
     1561 gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc atattgttct
     1621 cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa cttttctcca
     1681 cattaccact gctatttctt tggagattta actcattgat ttttatttac agttaaaaat
     1741 caaccttaca ggcattatag cgtactgttt tcgttttcac aattaa
//
LOCUS       DACCWV010000242         1777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCWV010000242 DACCWV010000000
VERSION     DACCWV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.249"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            12..1775
                     /locus_tag="IVW84_002915"
     CDS             12..1775
                     /locus_tag="IVW84_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919037.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT          575 a          334 c          300 g          568 t
ORIGIN      
        1 ttatattatc attgttattt ttaacaggaa ttgcttattt cttttatttt attttttctc
       61 ctgcggacag caatcaaatg aaagaaataa aagattcgtg ggttttctat acaaaagatg
      121 atcctgattt taagtttgac agcaagtaca ctcgtacgat tcctaccgtg gataaagacg
      181 aaacattcat tatggaaacg acacttgaaa aaccgttgga cgaagcaaat ttactaatca
      241 aaggaaacca tcaatggatt actgtatatc taggcgaaaa gattttatac caacggaaag
      301 attatcagga agaaagcaat cccggactct cattggctgt gatagattta ccagcaaatt
      361 atatcggaaa aaaattgacc cttacagtct catctcctta tcagaactat gctggactgc
      421 caccgaaagt ttatctagga acaactggac cattgattag ttttatttat tcacaatcag
      481 tgccgcaagt attgacgatg ataatcgctg tttttatcgc tatagggacc tttttctata
      541 cgatctactt gatgtataaa caaaaacgtc ttgaatactc acttattctc ttaagttgct
      601 ttgctttggc attaggattt gaagctgtat cagaagatat tttaagtggt attctttttg
      661 aaccattcgt tcattctttt ttatctcatt tatttattat cttaacgtca gctttcatca
      721 tctgctacta ttggagccga atgttttact ataaaaaatg gtacggaatt ttttgtacgt
      781 tccaactttt gatttttatc ggcgttctgc tttatgcaac ttttactcct gcagaacttc
      841 ctgaattaat gccgattgct aatattgcta gtgttataag cactttagtc acttcacttg
      901 catgcattgg cgaagcatat aagaataacc gtttttatgt agcttgtaca ccttggatcg
      961 tcttggtagc cattatccat tgcttgattt acatccaaac ggcattaggt atctatcaaa
     1021 caacgatcaa ttggtctacg attttatata ttatcattct aatcgttatt atcagctaca
     1081 atctaatcga tcacatctta aataccgaca aagatcggag acaagtagat ttcttaaaaa
     1141 tcaaaaatga tttgttggag caacattatg agcagcttcg acagcatatc caagaagtaa
     1201 atacacttcg cctggaattt gtccaagaga tggagaatct gcaagattta atgcatacgg
     1261 accaaacaaa agctaaaaac tatgtggaaa agatattaga agaagcaaaa aatttcgaaa
     1321 tgctgtgttc tttttgcaac caccaggtga caaatcttat cttagcgcgt taccagaagt
     1381 tagctgccaa acggaatatt cagataacct tccaagcaga tcttcccgaa gaacttccta
     1441 tcaacgatga cgatctggct caacttttga tccacgcttt agagcattcc tttagagaaa
     1501 ctcatgccat cgaaaatcca ctgtatcgaa aaatttattt gtcgatacac gaacaagagg
     1561 aacatttcgt catttgttgc gaacatagtg cacattacga tacaaaccta tttagtcgag
     1621 gcatcacaga agaattagat gatcaggaac gttttgactt aatgatgatg aaagacgtag
     1681 cggatcgtta cacaggaaca ctaacgcagg aaaaagatac tttcattgat cggatcactg
     1741 ttcaactctc tcgaaattac ccgaatcctg tataaaa
//
LOCUS       DACCWV010000243         1640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCWV010000243 DACCWV010000000
VERSION     DACCWV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.250"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            416..>1640
                     /gene="ltrA"
                     /locus_tag="IVW84_002916"
     CDS             416..>1640
                     /gene="ltrA"
                     /locus_tag="IVW84_002916"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7919038.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          586 a          299 c          388 g          367 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag
//
LOCUS       DACCWV010000244         1629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCWV010000244 DACCWV010000000
VERSION     DACCWV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.251"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..440
                     /locus_tag="IVW84_002917"
     CDS             <1..440
                     /locus_tag="IVW84_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7919039.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
     gene            complement(761..1510)
                     /locus_tag="IVW84_002918"
     CDS             complement(761..1510)
                     /locus_tag="IVW84_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7919040.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          518 a          322 c          308 g          481 t
ORIGIN      
        1 tcaaaaaagc cgaaggctct ccttgctttt tgttgtacca aacgttccct gggattggcg
       61 cactcacagc cgctttattg ctcggtgaat taggagatat cacacgattt aaaacccata
      121 aacaattgaa tgcctttgtc ggaatcgaca tccgacggta tcattcaggt aagtatactg
      181 gccaagaccg catcaataag cgtggtaatc ccaaagcacg gaaaattatt ttcttcacca
      241 ttcgaaatat gattcgccag caacgcgcag ctcctaatca tattgtcgat tattactaca
      301 aattaaaaaa gcaacctatc cctaagaaag ataaggttgc cacagttgct tgtatgaaca
      361 agcttctgaa atgtatgcac gctatggtta gggcacatac agagtacgac tatgcgtacg
      421 cggtctcagt ggaccattaa ctaaaccact tacagtatac cacttctttg cccagatcga
      481 aaatttttta cgaacggggc tttatttggt gtgctttgat tttgtcgctt tcgtttacga
      541 gctatttttc ttgcaccttt tacttgacta atcgtaggaa atagaggctg ggacagaagc
      601 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
      661 tcagcttatt tccgaaggag ttgcttctga ttccgccgtt tatacgtgtt tagagcgtga
      721 gacaaaagtg ttctttactt ttgtcccacg ctcagttttt ttatgcgtgt tgttgtgcgt
      781 tgtagtattc ttcttccatt ttttgcatac gttcacgttc tttttggtta taccaaggtg
      841 aaacggtaaa tgcaaggaca tcattcagca agtaaacaga gctgttcacg aacatcgcca
      901 aacttgcaga tccatgcgta aatgaaatat accaaaggac catttgagca agccctgaag
      961 ctaaccacca gtaatattgg ttgttgtaac gcaagaaact gatgatacca gcagtcaagc
     1021 taatagaaaa agcaatggca tctacccacg gccgaggatc atctgtataa cgaccaatca
     1081 ggaatcctga aacaagatag acaaggaaag tactgatgat tgcgataaac cagctttttc
     1141 cagtgaattt tcggattttt gaagccatat tatgattcca ttctttactt aacaatacgg
     1201 ggatatccaa tgtgatcata taagcaatct gttcaccgat acttaaatag ttctttgctg
     1261 aaaacccaac gataataaaa caaatcgcag acaaaatacc taaaataccg ttgactgatt
     1321 ttgccgcatt gatggataaa atacacaata caccaagcgt ggtaccgata aatgtaataa
     1381 cggacagcca agtgatggga ctattgacaa gagtcatgac ctggcagcca agactgaaaa
     1441 agaaaagata ataattttgt tgcggccagc cttttagctg atgagctaaa aactgaaaat
     1501 atgacttcat gtatgaggac tccttaagac actaaatgta gtgtgttctg tatttactaa
     1561 cacaatctat tgtatgcttt tcttgaaaaa aaactagtct ttcaattata aataatttgt
     1621 gttatgata
//
LOCUS       DACCWV010000245         1620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCWV010000245 DACCWV010000000
VERSION     DACCWV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.252"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            22..282
                     /locus_tag="IVW84_002919"
     CDS             22..282
                     /locus_tag="IVW84_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919041.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(644..883)
                     /locus_tag="IVW84_002920"
     CDS             complement(644..883)
                     /locus_tag="IVW84_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919042.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1169..1483
                     /locus_tag="IVW84_002921"
     CDS             1169..1483
                     /locus_tag="IVW84_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919043.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          559 a          220 c          285 g          556 t
ORIGIN      
        1 aacattaagg atggatgaaa tatgaataac gaaactatca ttttaggaaa aaaatatatt
       61 tgccaaccta ttggcttgaa acaaaaagtg attggagaag taatcagtaa attagaaaat
      121 tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc gcaatgaaat attagaaaaa
      181 tgcggaaaag tagtggcaaa atatactcac gtttatgagt tggaaacaac agaatatcta
      241 tataactata gcaacaaata tgaacatact ttagtttcat aaagatttaa ataaagtgcc
      301 ttcaaaagcg agaagaccaa gcttttgaag gcactttatt tagttggcag tgattgttca
      361 tgatttttga tttattcaaa aggttcgcct agagaatctg tctctgtaac gaggcctggt
      421 ggagaaactc gataaaaacc tatactgcca gaactgtcct attttcggat agacaaacta
      481 gtagttttaa aaagatcatc cgatccaatc atttcaaaaa atgcaaaact gatatcagga
      541 tcatttagct caggcttgtt atcgtaaagc atttgtaaag gttgcattta gttaggagta
      601 aacgcgaaac tatcatcatt atttgctgaa gaagatgaag aagctaattc agagtttgtt
      661 tgaaataaag gagacgtgct gacagtttct ttaccctgtt ttttgcagag gtagaaactt
      721 ttatatcgga agtttctacc ggagaatttt tctcatttgt agaaattgtt gaatcgatac
      781 tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc agaagtccca caagcagtaa
      841 gtaacaataa atttgataaa ctgataaaca gaattctttt cattatgtac acactcctta
      901 tagtatcgtt tagcatacac ctatgctcag tattaggcaa aatcaatgca aaactatcaa
      961 gtcttttata gtatctatta gtcttagtaa tacatatatg gagagggtga tatcccagtt
     1021 caaaccagat acattggtta ccatctgtat taacgattat gcctctaaac gcttttttta
     1081 tgaaaaaatt tataagtgaa aacgtaggta tgatattatc tagaggtaat ttttttgggt
     1141 atttaaatag ttgctaggag gatagctttt ggatttttac agtaatttta tcttaataat
     1201 agcaatatta ttacttctaa atatttggtt ttttgataag agtagaaatg cagggatagg
     1261 ttttagaacc aaacgaagta cgtcttctga aaaaaagtgg gtatattcac agactatttt
     1321 ttacggaggg gttatttcaa tcagtttact ttcttcaaca ctttattctt ttaatgttat
     1381 agacgtttcc atgtcaaatt ttatatctat tattggaatc cttatttctg caattattac
     1441 acaactactt cttgtgtttg aagagaagtc aaaaaataat taaagcaatt atgcctttta
     1501 gtacaggaat tattacttct tacaaatgta tagcgaatgt gaagcctgct gttttaattg
     1561 ttctagaaaa acttttgagt gaacaattat tttatgttag gatgaattaa tttctctaga
//
LOCUS       DACCWV010000246         1620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCWV010000246 DACCWV010000000
VERSION     DACCWV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.253"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            522..>1620
                     /locus_tag="IVW84_002922"
     CDS             522..>1620
                     /locus_tag="IVW84_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919044.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGEC"
BASE COUNT          546 a          159 c          288 g          627 t
ORIGIN      
        1 tcaccttcca acagtatata aaatgagcgt aaaaacggct atttttgcct taacaacgat
       61 ttttttgtat acaaatacga tatgctcatt aattattgga atttgtagtg ctgtttaaat
      121 taatgaaggt gtaattatat gagtgatcat tttgaaaaag tttgttgaga agatgtgctt
      181 ctagtcatgt gatgggagaa aataatttaa ctgttagagt gaatagttaa attattctgg
      241 ttatatgaaa aaagtagttt ctaaaataat cttgcaggga ttaacttaaa gaaattgaga
      301 gaatagtttc tttgaaattt ctatatacaa tgagaagctg aaatttactt ttatttgatg
      361 actggaaata gaaattaaat atattgtatt gttacattaa agaaaacttg tatggaaaaa
      421 tcagtacaat accattattt aatgttcttc acttaaagcg caaaaaatat cagtttatta
      481 aactcgtata tagaacaaat atatgtagac ggggcgaaac gatgagtgta aaaaaaatgc
      541 tatttatggt aagtacaata attgtattaa catcccgatt ttggggaatt aatactagtt
      601 tatctatgcg atattttatt atggcaatat gggatttata ttttatgtta agctattttt
      661 cttatcgtga gaaaacatat aaaaaatacg ttatcaagaa taacttttgg ttgactataa
      721 tgccgtttgt gatattttct atctatactc taataatatg gggaattggt tcaaatgcag
      781 aatttgaaaa ttttataaag ttatgttcca cgttgcttta tttaattctt gcatatggtt
      841 acgcatgtac agcattttac ttatttaagg cagaaggaat tgattttatt ttttggtcag
      901 gtgttatttc atatatgagt ggttcagttt tttatttaat tgtatcttat ggtttaaatg
      961 gtttaatttc ttatataaaa agtcttataa caggtgaaac aaatactgca aattttgcta
     1021 tggaggtaca tgatttaact tttgcgatgg gggttttttt cctttattac gtattttttg
     1081 aaaataaaaa tacaaaaaaa cataagataa aaattatcgt atctttaatt ttaattttca
     1141 tgggattgaa gagaattgaa ctgttagctt taagtggtgc aattctagtg tattatgttt
     1201 tattaaagtg gggaaaaaag attcaaatcc gtgcattagt gtgtactatt tgtgcaactg
     1261 ttatttcgtt agtgtttatt tacattatag atacaggaat tttggcattg cttatgtcaa
     1321 atttgggcat tacagatgac ggtgcaaggc tttcatttta tctatattca tctaaatttt
     1381 atgaacttgg tttaggatat gtaggaacag gatggacatg gttttcaaaa tactttttta
     1441 atctttattt atcgcacttt aggttaaatg gtggaagaat tgcagcaagc ctacacagtg
     1501 atatattaac tatttatata gaagttggat gtatagtttt tattatatgg atgttttggt
     1561 tattttttgt gaaaccgata agactaacta aaaaatttgg attgaaaaca ggggagtgtg
//
LOCUS       DACCWV010000247         1604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCWV010000247 DACCWV010000000
VERSION     DACCWV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.254"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            42..464
                     /locus_tag="IVW84_002923"
     CDS             42..464
                     /locus_tag="IVW84_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919045.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            474..677
                     /locus_tag="IVW84_002924"
     CDS             474..677
                     /locus_tag="IVW84_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919046.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            888..1472
                     /locus_tag="IVW84_002925"
     CDS             888..1472
                     /locus_tag="IVW84_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7919047.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          566 a          227 c          296 g          515 t
ORIGIN      
        1 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
       61 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
      121 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
      181 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
      241 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
      301 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
      361 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
      421 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
      481 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
      541 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
      601 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
      661 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
      721 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
      781 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
      841 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
      901 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
      961 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
     1021 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
     1081 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
     1141 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
     1201 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
     1261 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
     1321 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
     1381 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
     1441 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
     1501 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
     1561 ttattctcaa atgttgtaag gattttattc atttgttaat atta
//
LOCUS       DACCWV010000248         1600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCWV010000248 DACCWV010000000
VERSION     DACCWV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.255"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            76..1395
                     /locus_tag="IVW84_002926"
     CDS             76..1395
                     /locus_tag="IVW84_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP7919048.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            1367..1564
                     /locus_tag="IVW84_002927"
     CDS             1367..1564
                     /locus_tag="IVW84_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919049.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          573 a          245 c          360 g          422 t
ORIGIN      
        1 gttaagggct aaaaaggctg cactaccagt cttttagccc ttttcatttt gtttttgaac
       61 aagtaaaata ttactatgaa agcgaatatt ctcgaagcaa tcttttttga cgaacaccat
      121 aattgggata actttgttga gaaatacggg cgaaagattc ggctagtcgt caaacgggaa
      181 gtaaacaagt ttagatattg tggagatatt caaaaaggat accgactgtt tgtctgcgag
      241 ggatgtcatc aaacaaagtt tgttcccttg aaatgtcgtg gaaagttctg tccaacgtgt
      301 gcaacaggtg aaagccaaag gtggtcagcg gttgtggcga atgatatgtt taaagtgaca
      361 catcggcacg taatctttac gatcgatgag gggttacggg aaatctttat gatggaaaac
      421 tatcgagatt ccttactgaa aggactaatg gatgaagccg caaaaattat tcttgcctat
      481 tttgagaaaa gaaaggtgaa ggcaggtata gtagctacaa tccacacctt tggatcaaaa
      541 ctagaattta atccgcatgt tcatatggtt gttactatgg gtggcttaac aagagatgga
      601 agttgggaag aatacgatta tttacctttt tctatgttaa gaaaatactg gaaaaatgct
      661 gtattgaaac taattagacg aacgttaggt gagtgggaca agcaaagagt tcaatcaaga
      721 ttacaagctg cgtacaagaa caatggagaa gggttttacg taaatgctcc taaaaggagc
      781 aggacaaatc taaagtcact actccaatac atcagtcgtt atatgaagcg tggcccgatc
      841 gcattaagcc gaatcttaat gtatgatggc gacactgtca tgttcaacta catggacaaa
      901 cgaacgaata ctaaagagac gatgaccatg agtatagacg aattcatagc tgcactgatt
      961 cgccatattc aaaataagaa tttcaaaacg attcgtcgct atggaatcta tagtcgtaga
     1021 atcaaaacat taatgaaaaa ggtcatgaag gagtatcaga aacgagtaaa aaagctgttt
     1081 atcaatctga atgaggcctt aagaccaaaa tcctgggaag aaagaataac cgaagaattt
     1141 ggtgaaaatc ccttagaatg ttcttgttgc ggtgagtact atgaattcgt tgggatgagt
     1201 gtgcgcaaaa atggacggtt agttattaaa tatgcgaaag ataaccatgc aaagcggttt
     1261 atgcgagagg agaatcgaaa aattgaccaa aaagaattca ggcaacagta tgaagaagaa
     1321 aaagaaaaaa cttttaaagc aattcgctgg gattgggaac gccaacgtga aatttatatg
     1381 tctaagttgt ggtgaaaaag aatcaatccc aatggatgta gtaaaatttt tagatgatgc
     1441 agatattgca aatgatccga acaatcctcc ccaatttacc tgtgaaaaat gtgggaagga
     1501 gatgtatcca gaatattata gaaacgccct tggaatagag tttaagatca gtgatgttca
     1561 ataagaaggt taatcataca aaaagtccac cggagaattc
//
LOCUS       DACCWV010000249         1583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCWV010000249 DACCWV010000000
VERSION     DACCWV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.256"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            40..1533
                     /gene="guaA"
                     /locus_tag="IVW84_002928"
     CDS             40..1533
                     /gene="guaA"
                     /locus_tag="IVW84_002928"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7919050.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          482 a          325 c          356 g          420 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taagacgtaa atataatatg gatggggaat
     1561 gccgataaat caacgttttt caa
//
LOCUS       DACCWV010000250         1566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCWV010000250 DACCWV010000000
VERSION     DACCWV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.257"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            101..1327
                     /locus_tag="IVW84_002929"
     CDS             101..1327
                     /locus_tag="IVW84_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919051.1"
                     /translation="MIQPTFIRSFTQQADPYDLRKLQEKIMLASVRDESWLVVGNDEN
                     ECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTK
                     QISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYY
                     HQIIPKPIFLDIQQVEKVKELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHL
                     AWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKE
                     RRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEK
                     YQEYLLSLRIYVPSESLSELPLKEIKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQ
                     FLDYRELEQLITLQKI"
     gene            1338..1547
                     /locus_tag="IVW84_002930"
     CDS             1338..1547
                     /locus_tag="IVW84_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919052.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT          536 a          264 c          299 g          467 t
ORIGIN      
        1 gattggcgaa gataaattat tgatgactat ccagctcaca gacttaaatg aagaagaaaa
       61 aaaacaggaa ataagcgatt gggctatttt gacacgtctg ttgatccagc caacatttat
      121 ccgctctttc acacaacaag cggatccgta tgatcttcga aagcttcaag aaaaaataat
      181 gctagcatcg gtaagagacg aaagttggtt agttgtagga aatgacgaga acgaatgtag
      241 ttttcgcttg gaagataatc aattattgat aaaaaatgta ttgtctatct ctgttttcaa
      301 agaaaagcaa tttttgattc gagacttcat tcagaaaaaa atgattgctc atggggtgtt
      361 cgcctatatg cgagcttata gcgagtttat ctatcacaat acaaaacaga tctctcaacg
      421 gctattgttt gagaaaaagg atgaaatcga acatttacct aaaatgaaac aacaaaacgg
      481 ggaagtagta gtcgattgca accaatttcc aggatacgat cttttttacc aaggtttatg
      541 tttcacttcg tgttgggaaa tgtattatag tcgctattat catcaaatca tccctaaacc
      601 gatttttctc gacatccaac aagtagaaaa agtgaaggaa ttagaaaacg aagtcatatg
      661 tatccagctt tatcgagacc cctttaactg gcaaaagcca aacaatcaaa tgtttcaatc
      721 ctattttagg gaccagcttg ggtttgatca tcttgcttgg gacaatggtg tcggtttatt
      781 gaaaccgcca ttcgttgaat acatttacac tgaccatacg atccaatctg tccagtatca
      841 gaatgcacaa atgcaacctg ttcctaaaaa gaatgcttca ttttttgtaa caaaaagtta
      901 tgatattcaa caaggagatt ataaagaaag gcgtgttcgc ggaacactaa atgctcaggc
      961 gtattttcct tgggttgacg agaatcgctc gcgcatgatg tgctataaag ttatcgatcc
     1021 gactgttgct ttggataatg gaatcgaagc ttattgttat tatattaggg aatatctaga
     1081 agtagaggta acagatgaaa aatatcaaga gtatctttta tccttaagaa tctatgttcc
     1141 ttcagaatcc ctgtcagaat taccgttaaa ggaaatcaaa catagactct ctgatgttac
     1201 cttcaaacga ataaagaaaa agcggcgaag tatccaattc gatgtcaaga aaggtgagca
     1261 gcatctgcgt gtgcaatttt tagattaccg agaattagaa caattgatta ctttacaaaa
     1321 aatatgaggt gaagaaaatg gataaacaac tctattatcg aataatggtt ccggcatcat
     1381 tattatccgt ctgtctcttt tttgttccta attttcctat tcgatcagtc atcggttttt
     1441 tggtcatgtt tggcgggatg ggactttatt attatcttct tcataaggaa agaaaacctg
     1501 cttctgttta tatgaaaaaa acaagaagcg aaaataaaaa acgataaaac ggcttgatga
     1561 tagaca
//
LOCUS       DACCWV010000251         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCWV010000251 DACCWV010000000
VERSION     DACCWV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.258"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            22..1488
                     /locus_tag="IVW84_002931"
     CDS             22..1488
                     /locus_tag="IVW84_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7919053.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          326 g          497 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ctgt
//
LOCUS       DACCWV010000252         1532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCWV010000252 DACCWV010000000
VERSION     DACCWV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.259"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(502..1116)
                     /locus_tag="IVW84_002932"
     CDS             complement(502..1116)
                     /locus_tag="IVW84_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7919054.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          505 a          288 c          246 g          493 t
ORIGIN      
        1 attgaatttc attgcaaaaa aaataataaa cacttataaa tatccattat ctatccttca
       61 atttttgtgt attttatttc tattagaaaa aggtatcctg ttggacaatt ttgttatatc
      121 aggtaaactt cattgcaata atgataacaa atgtactata attactttgt aaagagtttt
      181 ggggaagact ctttcatcaa cgtgtattaa gagacaagaa agaatccata tggattcttt
      241 cttgtctctt tttccactac tttttatttt tctgacacta catattctta taactcattt
      301 ctcataactg tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc
      361 aaagatgatc catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta
      421 ataatcgttt tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga
      481 actttgtcaa catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt
      541 aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc
      601 tggatctaga tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac
      661 caaatcagca aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga
      721 caaatcaaat ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag
      781 ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt
      841 gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc
      901 ggctggattt tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa
      961 tgctacacca cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg
     1021 tttcgcgatc caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc
     1081 caattggtga taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact
     1141 tgacatgaat agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac
     1201 agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca
     1261 ataccttgac atagcaaata aaacaacgtg cagaccatgg aaaatatcta cataggggta
     1321 gaattttcac cctagattag gtacaataaa ttaggtcgat gcactgcatt gactatggca
     1381 aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc ctgattctta gcgccaacta
     1441 agggtcaggg agcctttttt tattcagctg tttctatctg aatttattgt aagtaatctc
     1501 ttatccaagt gcaagcaaga aaatacaaaa aa
//
LOCUS       DACCWV010000253         1531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCWV010000253 DACCWV010000000
VERSION     DACCWV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.260"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            289..696
                     /locus_tag="IVW84_002933"
     CDS             289..696
                     /locus_tag="IVW84_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919055.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="IVW84_002934"
     CDS             710..1111
                     /locus_tag="IVW84_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919056.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="IVW84_002935"
     CDS             1113..1484
                     /locus_tag="IVW84_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919057.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          579 a          186 c          265 g          501 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt a
//
LOCUS       DACCWV010000254         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCWV010000254 DACCWV010000000
VERSION     DACCWV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.261"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVW84_002936"
     CDS             131..505
                     /locus_tag="IVW84_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7919058.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVW84_002937"
     CDS             complement(619..1248)
                     /locus_tag="IVW84_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7919059.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVW84_002938"
     tRNA            complement(1382..1454)
                     /locus_tag="IVW84_002938"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          513 a          228 c          264 g          504 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaa
//
LOCUS       DACCWV010000255         1500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCWV010000255 DACCWV010000000
VERSION     DACCWV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.262"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1233)
                     /locus_tag="IVW84_002939"
     rRNA            complement(<1..1233)
                     /locus_tag="IVW84_002939"
                     /product="23S ribosomal RNA"
BASE COUNT          344 a          411 c          284 g          461 t
ORIGIN      
        1 accaatacac ctaaaggtgt actccacagc ttcggtaata tgtttagccc cggtacattt
       61 tcggcgcagg gtcactcgac tagtgagcta ttacgcactc tttaaatggt ggctgcttct
      121 aagccaacat cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg
      181 ggaccttagc tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt
      241 ctgactcccg gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat
      301 gggcccctag tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa
      361 agctatttcg gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca
      421 cctcatcccc gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc
      481 ttcaacctgg acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc
      541 ctattcagac tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt
      601 aactcgccgg ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt
      661 gtaggcacac ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc
      721 tcacggtact ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc
      781 cggattccga cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca
      841 catttcgtct acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct
      901 gaaagaacta ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt
      961 ttcccgtttc gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact
     1021 tagatgtttc agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca
     1081 tcctattaaa gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag
     1141 ctccccaaag catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc
     1201 accgtgcgcc cttattcact taaccttatc aaccttacgg ttgggttttg atccttcttc
     1261 tagcgataga agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag
     1321 ctcacaaatc agcctgtacg gcaaaaagat cgatcgtctc ataaaatcaa agattttatg
     1381 gccgtccgcc tattttgctt caggctaaac aatttgttca gctttcaaat tcaacgcggt
     1441 gttctcggtt tgtattactt acatttactt caaatatcca gttttcaatg aacaaaagta
//
LOCUS       DACCWV010000256         1488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCWV010000256 DACCWV010000000
VERSION     DACCWV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.263"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..1463)
                     /locus_tag="IVW84_002940"
     CDS             complement(36..1463)
                     /locus_tag="IVW84_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7919060.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          588 a          252 c          173 g          475 t
ORIGIN      
        1 gtaaaggagt ccaagcaatg aaagctgtct tcatattaat tctccttttt taaatacaag
       61 taagaaatgc ttcccaaata ataccagaaa acaattgaaa tgacatcgcg cttattgaaa
      121 agtacatgag cctccaccat ttcatctata aaaatagata gtataaaaat taaaacaata
      181 ccaagaaacg cataattcac tttacttcta aaaaatgttt tgagtaaact aaagatattt
      241 aacacataga aagcatagat acatataaaa gcaattatac cacttgatac aagtacttga
      301 agaaatccat tatgcatgtt tccatgtgct ctttttagtt cattcacatc tgtagtatca
      361 agatctactt ttttcaaaaa ttttgcatcg ctattcccat agaaatcggt ctctccataa
      421 ccaaataaca aagttggtct tataacttta agcgcagctt tccaaatcgt aaaacgacca
      481 gctgaaattt cggagttatc actagaatgc tgtaatatta cttctttttc tggaagtgga
      541 gttttatctg attcatttga ctctataact tctacttttc tatcaatcga tacaggaatt
      601 gttgctaaaa aatccttagc ataattagta ccaaaagaaa aatataccaa tagaactaat
      661 aatatgctta ccatccattt gatttttaca ggtagtactt ctctaatgct ctttctaaat
      721 tttgaattgg caaacaacaa aagaaataat actaaaaaag aacaaattac tacctgggca
      781 cctctagacg aagaaagtgt aatataaaat atttgtacga taaaattcat catataaaaa
      841 acacgaatcg tttttgttat ttttgaaatt aatacactaa ttaaacttaa aataatagaa
      901 gtatatccaa acatagctcc aacatttgga cttgtataga caccaaataa ccgatgttca
      961 ataaaacctt gtcttgcaag taatcctggc gttcctgttg ggacatgata agaaatcaaa
     1021 aaaataaatt gatagataga tattagcgca gctaaaaaaa ttatgataat gaaaacatta
     1081 ttaaaataca ccattttttt ctttttttcc tcttcacttt gtgaaaagtc tgcaggaaaa
     1141 attactatca aagtaatcag caaatatcca aaattataaa tagatggaat taaaaaattt
     1201 tctctgttaa tgattatagt taagataaaa ctaatcattg ctccaatcag tagccaagaa
     1261 aaacgcattt taaaaaaatt tttctttata aaaacctcat acaaaactat aattccaccc
     1321 caagcaaaag ctattttcat cgggatatta gttacattaa aagtactagg cataaaataa
     1381 agcagacaaa tgattaaatt tattactcga aacgcatctc gacttaataa actcttctct
     1441 aaaacaggta ctattctatt catctatatt tccaactttc aaaatttc
//
LOCUS       DACCWV010000257         1482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCWV010000257 DACCWV010000000
VERSION     DACCWV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.264"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          553 a          169 c          303 g          457 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aa
//
LOCUS       DACCWV010000258         1466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCWV010000258 DACCWV010000000
VERSION     DACCWV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.265"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            58..912
                     /locus_tag="IVW84_002941"
     CDS             58..912
                     /locus_tag="IVW84_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7919061.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1009..>1466
                     /locus_tag="IVW84_002942"
     CDS             1009..>1466
                     /locus_tag="IVW84_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7919062.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQV"
BASE COUNT          555 a          197 c          246 g          468 t
ORIGIN      
        1 taataatata aacataataa aacaaaaatt atccaaatct aacataggag ggtggatatg
       61 gaaaattcta tatatttagt aacagaacct aacactcagc tgaaagattg ttactttaat
      121 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
      181 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      241 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      301 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      361 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      421 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      481 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      541 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      601 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      661 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      721 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      781 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      841 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      901 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      961 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
     1021 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
     1081 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1141 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1201 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1261 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1321 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1381 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1441 gattcagtta caaatgttca agtaga
//
LOCUS       DACCWV010000259         1465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCWV010000259 DACCWV010000000
VERSION     DACCWV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.266"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..753
                     /gene="aph(2'')-I"
                     /locus_tag="IVW84_002943"
                     /pseudo
     CDS             1..753
                     /gene="aph(2'')-I"
                     /locus_tag="IVW84_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            737..1129
                     /locus_tag="IVW84_002944"
     CDS             737..1129
                     /locus_tag="IVW84_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7919063.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1130..>1465
                     /locus_tag="IVW84_002945"
     CDS             1130..>1465
                     /locus_tag="IVW84_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7919064.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          601 a          106 c          250 g          508 t
ORIGIN      
        1 attgaaataa tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt
       61 tttaaaacaa aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat
      121 aattttttaa atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat
      181 attagtgatg aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca
      241 gaaatttatt ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt
      301 tttttaagac aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat
      361 aaacaaaatg tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact
      421 gatatagaaa aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt
      481 gagggtaaaa agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc
      541 aataatagat taactggaat aattgatttt ggagattctg gaattataga tgaatattgt
      601 gattttatat acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat
      661 atattaagaa tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt
      721 gaagaatatt atcctattga aactattgtt tattgatgat aatatggttt ttgatattga
      781 taatttaaaa ggatttctta atgataccag ttcatttggg tttatagcta aagaaaataa
      841 taaaattata ggatttgcat attgctatac acttttaaga cctgatggaa aaacaatgtt
      901 ttatttacac tcaataggaa tgttacctaa ctatcaagac aaaggttatg gttcaaaatt
      961 attatctttt attaaggaat attctaaaga gattggttgt tctgaaatgt ttttaataac
     1021 tgataaaggt aatcctagag cttgccatgt atatgaaaaa ttaggtggta aaaatgatta
     1081 taaagatgaa atagtatatg tatatgatta tgaaaaaggt gataaataaa tgaatatagt
     1141 tgaaaatgaa atatgtataa gaactttaat agatgatgat tttcctttga tgttaaaatg
     1201 gttaactgat gaaagagtat tagaatttta tgatggtaga gataaaaaat atacattaga
     1261 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
     1321 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
     1381 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
     1441 gccaaattat tggagtaaag gaatt
//
LOCUS       DACCWV010000260         1443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCWV010000260 DACCWV010000000
VERSION     DACCWV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.267"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            90..1430
                     /locus_tag="IVW84_002946"
     CDS             90..1430
                     /locus_tag="IVW84_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7919065.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          457 a          318 c          282 g          386 t
ORIGIN      
        1 actttttctt ttttctaata aacgcaaaca gacaccttga aatcctttag aataaagtcg
       61 acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc aaagagttac
      121 tagatattct agacctaaat cttacctttc atgaagatgc ctttcgcaaa gagcgcatta
      181 atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccagag gaatgcttcc
      241 attgccacta tcaaaataag caaacgatca ttaaatgggg ctggaaaaaa gtatccatct
      301 tattgaatga tgtcagtaac tacaagacca ttgtgcggat taacaaacag cgcttcaaat
      361 gtaaacactg tggaaagact tttttagcag aagattcagt tagcgatcgt cgctgctcca
      421 ttgcccgcag agtcaaacaa gccattcttg agctattaag tgagcctatt tctatgtctt
      481 tgattgcacg tatgaagcac atttctccga ccactgtcat tcgaatactt cgcagccttc
      541 ggcctaaaac cgtttctttg aaccagccgt taccagaagt ggtgtgtttc gatgaattca
      601 aatcggtgaa aaacgtctcg ggagccatga gctttgtcat gatggacggc aagacccatc
      661 aactgattga tattgtggaa aatcggcaac tcaatccttt acgcgactat ttcttgcgct
      721 accctagaaa agtccgtgaa caagtacgtc tagtggtttc agatttctat acaccctatc
      781 gcacattggt gaaagagtgt tttccgaatg cccgaatcgt cgccgatcgc tttcatatta
      841 gccaacacat cggtcgggcc tttaccaacc accgaattca agtaatgaag acctttaaaa
      901 aaggggatcg tcggtcgaag cacttaaaga aatactggag actacttcaa aagaatgcgt
      961 gggaattgaa tggtcagcat cgctactggc gtccgtcatt tcgagatcat ctgacagaag
     1021 cggagatagt cgatcggcta ttatattatg atgactcctt aaagcgaggc tacgaagtct
     1081 accaggtttt tctttccgct atcagaagac aagatgttcc tgaattcgtc gcgctcctaa
     1141 aagaagacta caaggagttg ccagagcact atcaaccagt gttcacgacc ttcaagaaat
     1201 atcgaacaga aatcaaacga gcgttgcgag ttccctattc caacggccct atagaatgtt
     1261 tgaacaacca tatcaaagta ctaaaacgaa ttgcctatgg ctttagaaac tttcaaaact
     1321 accgagaacg aatcttttta tatcgaggaa aatatttcaa gaaaactaag aacactaccc
     1381 aactgactaa agccagaaca acaacacggc tagacaagat agctgtctag ccgtgtgagt
     1441 atc
//
LOCUS       DACCWV010000261         1427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCWV010000261 DACCWV010000000
VERSION     DACCWV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.268"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(150..1337)
                     /locus_tag="IVW84_002947"
     CDS             complement(150..1337)
                     /locus_tag="IVW84_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7919066.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          415 a          311 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacac
//
LOCUS       DACCWV010000262         1420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCWV010000262 DACCWV010000000
VERSION     DACCWV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.269"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            35..445
                     /locus_tag="IVW84_002948"
     CDS             35..445
                     /locus_tag="IVW84_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7919067.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            459..815
                     /locus_tag="IVW84_002949"
     CDS             459..815
                     /locus_tag="IVW84_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7919068.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          523 a          208 c          266 g          423 t
ORIGIN      
        1 tagaattgga ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag
       61 ctgtacttcc tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga
      121 gaagaatatt tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac
      181 tgaaggagga gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat
      241 agattttaac gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt
      301 attgagacga cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga
      361 gatccggtca tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca
      421 aaacaataac atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat
      481 cagcgcactg tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt
      541 atcaatttgt tgacttagaa gaacgcgatt taagtacaga agaagcagaa gaattatgca
      601 gttttgaaga agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca
      661 ttgcgattga cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata
      721 ccaatctcct cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg
      781 agcagttgat ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac
      841 tgagcatttc atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt
      901 tttttgactt tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat
      961 ctaggtgggg atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca
     1021 tttctgttgt tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt
     1081 tgttgacatg ttagaaatga catgataaga taactgagtt gttaaaaaag aaccacttgt
     1141 gaagcttgat tctatcgaaa aaaacttcaa aaaagttctt gacaaataac aaatgataag
     1201 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1261 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg
     1321 aatcggggaa cactcctgat gaagtagacc tttgaaaact gaacaaagta agacaaacca
     1381 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca
//
LOCUS       DACCWV010000263         1411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCWV010000263 DACCWV010000000
VERSION     DACCWV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.270"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            52..1347
                     /locus_tag="IVW84_002950"
     CDS             52..1347
                     /locus_tag="IVW84_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7919069.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          438 a          281 c          265 g          427 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt
     1321 catggcaaaa catttttaac caaataaaca gaacagaaag aggttttctt ctttctgttc
     1381 tgtccaaatt attctaagat ttctctttac c
//
LOCUS       DACCWV010000264         1377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCWV010000264 DACCWV010000000
VERSION     DACCWV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.271"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(680..753)
                     /locus_tag="IVW84_002951"
     tRNA            complement(680..753)
                     /locus_tag="IVW84_002951"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Ile,seq:cat)
     gene            complement(943..>1377)
                     /locus_tag="IVW84_002952"
     CDS             complement(943..>1377)
                     /locus_tag="IVW84_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7919070.1"
                     /translation="TDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENA
                     NLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFN
                     MRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          426 a          251 c          241 g          459 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      721 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      781 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      841 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaataa tttttatagt
      901 tgtcaatata gttatgtact gctcctcaac gaggagctat ttttatcgtt taggaatatt
      961 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1021 attcgttgcc cgcatcatta catagacttt cttactagga aaattacgca tattgaaaga
     1081 tacatgataa ccaacatttc cagaagtatt ataagcttgg tttacatctg gtctataaat
     1141 tccatcagct cttactcgag ctaattcttt cccagtattg tagtccataa tgaaaatata
     1201 ctcgtattta tagttagcaa tgtgccatcc agccacatgc aagtttgcgt tttcgatttc
     1261 cccaaactga tcaatgtggg cgtgatttgt tccatctgtc agcgtaggat tagctgcacc
     1321 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggt
//
LOCUS       DACCWV010000265         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCWV010000265 DACCWV010000000
VERSION     DACCWV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.272"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="IVW84_002953"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="IVW84_002953"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7919071.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..>1368
                     /locus_tag="IVW84_002954"
     CDS             1131..>1368
                     /locus_tag="IVW84_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919072.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEF"
BASE COUNT          421 a          274 c          207 g          466 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttc
//
LOCUS       DACCWV010000266         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCWV010000266 DACCWV010000000
VERSION     DACCWV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.273"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            82..>1364
                     /locus_tag="IVW84_002955"
     CDS             82..>1364
                     /locus_tag="IVW84_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7919073.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACCWV010000267         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCWV010000267 DACCWV010000000
VERSION     DACCWV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.274"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1337
                     /locus_tag="IVW84_002956"
     rRNA            <1..>1337
                     /locus_tag="IVW84_002956"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACCWV010000268         1330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCWV010000268 DACCWV010000000
VERSION     DACCWV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.275"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(18..347)
                     /locus_tag="IVW84_002957"
     CDS             complement(18..347)
                     /locus_tag="IVW84_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7919074.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(424..696)
                     /locus_tag="IVW84_002958"
     CDS             complement(424..696)
                     /locus_tag="IVW84_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7919075.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(696..959)
                     /locus_tag="IVW84_002959"
     CDS             complement(696..959)
                     /locus_tag="IVW84_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7919076.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1193..>1330
                     /locus_tag="IVW84_002960"
     CDS             1193..>1330
                     /locus_tag="IVW84_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7919077.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          424 a          256 c          172 g          478 t
ORIGIN      
        1 gttttgaagg aacgatttca taagccaatt tctgttgcca actcctcttt agtcattact
       61 ttagaattag taagtgtatc ttcagcaata tctctaaagg aagaatgcat tttgtttttt
      121 ctattcttaa attctacaag aatctcatct ttatcgtagc cttctgcaat tatatcagac
      181 aaaatatctt cgctaaaatc ataaaattct ttttgtggtt caacaaattc ataaagaata
      241 ccattctcta ccaactttgc ttcaacttcg actccattag gaacattaaa atctttaggt
      301 actgtcaaca tgattgaatt gccttgtttt cttgtttttg taatcattta aatccctcat
      361 ttctaacagt atgtaattac ttagtaaata cattatacca cgagataatg tatttacaag
      421 taattactgt gactcgtaat gcgtccttgc tgaataaatt tcaaccacgt gttcatcttc
      481 atctacctta taaacaaccc ggtgttgtct attcaatctt ctagaatacc taccttcatg
      541 tgtaggtttt aacttctcaa atgattgggt agggagatag ggatcttcct ttaaagtctg
      601 aataaccttc tcaaattgag gtttcaaatt agactgtttg attttcttta aatcagcttt
      661 agccgagttt ttaatcatca cagaatagtt attcattata agttatccca atcaatttca
      721 tcaacattag taaatccact atcatctttt tctcgcttac gaacaacacc caaagtgcct
      781 atttgctcaa gtaatagcgt ttctttgatt gctttccaat catccaaacc aataagcacc
      841 gcattatttt cactgcgctc actaatgatt tcaatttccg tatgattctc atttacatct
      901 ttcaacaact tataaaaatt tttccgtgct gagctaggat ttaagactgt ttttgccata
      961 tctactcccc ctctaattta tatctccatt ctaacgtact taattaagta cgtaaattcg
     1021 attgttttgt ttaaattgga gtaccaaaac agcattgtaa agtgttatcc gtttccttta
     1081 tacccgtggt gctagttaat tttttcctag aggaaaatgt ctaaacatgt tggtaaatca
     1141 acaaaaaaac gcatagagta gtctcaaaac tcccaagaaa tgaggactac atatgcgttc
     1201 atcaaaatat actgaacatt atacagatac ttttataaca ttcaaggaaa ttatgagaac
     1261 tgtttctaac atataccaca attgtgtacc agataagatt aaaaaccgaa gaaatactga
     1321 tcaactgaag
//
LOCUS       DACCWV010000269         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCWV010000269 DACCWV010000000
VERSION     DACCWV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.276"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            59..1066
                     /locus_tag="IVW84_002961"
     CDS             59..1066
                     /locus_tag="IVW84_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7919078.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1078..>1322
                     /locus_tag="IVW84_002962"
     CDS             1078..>1322
                     /locus_tag="IVW84_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7919079.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSD"
BASE COUNT          523 a          167 c          247 g          385 t
ORIGIN      
        1 ttttttaaag attaagaatc gaattataga tgactataat tcataggagg agatataagt
       61 gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt
      121 tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa
      181 acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga
      241 tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc
      301 tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa
      361 tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt
      421 aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt
      481 gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc
      541 ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa
      601 acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat
      661 gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga
      721 aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa
      781 aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag
      841 gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga
      901 aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga
      961 ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca
     1021 ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg
     1081 aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta
     1141 gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca
     1201 tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat
     1261 cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat
     1321 tt
//
LOCUS       DACCWV010000270         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCWV010000270 DACCWV010000000
VERSION     DACCWV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.277"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..752)
                     /locus_tag="IVW84_002963"
     CDS             complement(<1..752)
                     /locus_tag="IVW84_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7919080.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIV"
     gene            923..1291
                     /locus_tag="IVW84_002964"
     CDS             923..1291
                     /locus_tag="IVW84_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7919081.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
BASE COUNT          381 a          195 c          254 g          488 t
ORIGIN      
        1 acgattgctg aaaaagtctg attcatttgt tctactagtt ttaattgaat gcgagaagta
       61 gtcattgcct gctctgtttc caccaataca tcacgtaaat gatttttcaa agcatgattt
      121 tcttggaaaa gtttattttg cgctagttct ttcaatgcat ctaaattagc agaagtcgct
      181 tctctgaaat aaacgaggct tttttggata tccatgattt gataaagttg tttgttttca
      241 gtggatactt gaagtttggc ttccaaagta tcgcattgag attgaagcgt agctaaataa
      301 ctgttgtaag agagtgtgac ctgccaaatg aattgaaaga agatattgag attaggcgaa
      361 atatcacttt ttggaaaggt ctggctaaag actttcttta aaaacagtgg ttcatgattc
      421 gtaattgtga taagcttctt tttgggcgtc aaaataatag agagcgggta tgtatccact
      481 tgaaaataac cgctaggact tgttttagca aaaggatatt gtaacaataa taacgctggc
      541 tttgtcaact ttgattgatt gaattcttct gtacgggaat tttctgcatc gtccaatatg
      601 gaagtgatgt aatctgacgg aatttcaaat tgatgtgtca gctgatgaat ctcttcttga
      661 ttaggacgtt ctacacaata ccagatcgtt tggtcagttt cctttccggt attacgcagt
      721 ttctcattat ctaatgtaaa atatttgatc atgattttcc tcctgttgcc tgtttctttt
      781 attataacgg atagggaatt ggaatacatg gaatgtgttt gtaagatata gaatataact
      841 gcataaattt aaataaaatg aaacagttgt gtaaagtata tacagtatac ttgaagcaac
      901 gataaaaaag gagtttacgt tcatgggcac attcacacca cttatcttgt tttggtatat
      961 ttacccggta atcgttttgt ttggttgcca atttcttgta aaattatttt cgttaaatcg
     1021 tcgttttaaa ataaaagccc ctgatttggc gattcctttt ttgtggggcg gtatgcatgc
     1081 attgtctaga aatacaggga caatttctat cttaccgttt ttctttattt ctgttttgct
     1141 aatgggaatt tgtattgctg ttttccaagc atattattat gaagaaatta tttatccgcg
     1201 ttatttcaag atgacttggc gtttggtttt tctgttgaca atcgtcttgt acgctgtctt
     1261 gatcattgtg aacatacttt cttacctatg aaaaaaatag aaaagctgta aaaagtct
//
LOCUS       DACCWV010000271         1306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCWV010000271 DACCWV010000000
VERSION     DACCWV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.278"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(42..1168)
                     /locus_tag="IVW84_002965"
     CDS             complement(join(42..940,940..1168))
                     /locus_tag="IVW84_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7919082.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          376 a          223 c          206 g          501 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac acttctaggg
      841 atttctaata accgacataa caaagaaaca gtgtaatcct tacaccattt ttcaataaat
      901 tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt aagatttcaa
      961 gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt ccagtcagtc
     1021 ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct tcagataagc
     1081 catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca acaatcattt
     1141 gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat aagtttaatt
     1201 tattcttgtc tctacgaaaa gctgtctaag atttgatact aacaccaagt tatctttaga
     1261 accatttcag atacatatga aacaattaca tatgattttt tcataa
//
LOCUS       DACCWV010000272         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCWV010000272 DACCWV010000000
VERSION     DACCWV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.279"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            89..1129
                     /locus_tag="IVW84_002966"
     CDS             89..1129
                     /locus_tag="IVW84_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7919083.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          527 a          185 c          206 g          374 t
ORIGIN      
        1 cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat
       61 aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta aggcggatag
      121 agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg acgaatacaa
      181 aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat tagagatcgc
      241 aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata cgacaaatga
      301 actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga
      361 atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag ggaaaaataa
      421 tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct acttactcga
      481 aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga aaatcatacc
      541 caccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta tgaaaatcat
      601 accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac ttaataataa
      661 tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac aagatcaatt
      721 gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt ttgtccctga
      781 acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc aaaaaaccgt
      841 ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtgatattt cgatagtttt
      901 tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga cactgttaaa
      961 agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta
     1021 tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt
     1081 gaattatgca agctccccaa gcactattac gaaagactgg ttagagtgaa actttactga
     1141 caaagtgatt gttgcttcat ggagtaacat taaaattaaa aattttttat tattctaatt
     1201 atattacgaa tattacaaaa taatagctat tttaatagaa tttataagtt aaaatattat
     1261 gataaaaatc acaaacgtag ccttttacgt ca
//
LOCUS       DACCWV010000273         1278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCWV010000273 DACCWV010000000
VERSION     DACCWV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.280"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            825..1037
                     /locus_tag="IVW84_002967"
     CDS             825..1037
                     /locus_tag="IVW84_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919084.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
BASE COUNT          470 a          194 c          214 g          400 t
ORIGIN      
        1 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
       61 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
      121 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
      181 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
      241 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
      301 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      361 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      421 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      481 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      541 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      601 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      661 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      721 aataaaaaat aaaaaaatga ataaaaacgt tttcaaatac aaggtaattt gttattataa
      781 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      841 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      901 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
      961 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcaccta
     1021 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
     1081 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
     1141 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
     1201 atcaagagaa agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt
     1261 ggcaggttat gcgttttt
//
LOCUS       DACCWV010000274         1268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCWV010000274 DACCWV010000000
VERSION     DACCWV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.281"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(55..>1268)
                     /gene="cadA"
                     /locus_tag="IVW84_002968"
     CDS             complement(55..>1268)
                     /gene="cadA"
                     /locus_tag="IVW84_002968"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7919085.1"
                     /translation="ELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT          364 a          291 c          214 g          399 t
ORIGIN      
        1 tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctcttca ttgatcaacg
       61 tttatttttt acatgcaagc ttcgcatggc attcaaaaca gctaatactg tcaccccaac
      121 atctgcaaat actgcatctc ccattgatgc aaaaccaaaa gcacctaagg caagtaccaa
      181 aatttttatc ccaatagcaa agacaatgtt ctgtttgacg attttcaatg ttttctttgc
      241 taaatggatt gcttctgcaa tcttcgatgg ttgatcattc atgatgacta catctgctgc
      301 ttcaattgca gcatcgcttc ccaatccacc cattgcgatt cctacatctg ctcttgctag
      361 caccggtgca tcgttgattc catctccgac aaatgcggtc tttttattgt ttctttgtaa
      421 gatttcctct aaccgctgca ctttatcttc tggaagcaat tcgctatata tcttgtccac
      481 gcctacttgc ttcccgatat gatcagcaat ttttttcgaa tcgcccgtca acatgactgt
      541 gttcttcaca ccagcctttt ttaattgaac caatgcgtct acggcatctt ctttcaaggt
      601 atctgcaata acaagataac cactgtaact ttgatcgatc gctagataaa gcaatgtacc
      661 gatttcttga cttgaaggaa aagagatttc aaatctttcc atcaatttgt gattacctgc
      721 taatacttgt ttgccatcaa cttcagctaa gatcccttca cctgcaaatt cttggatatt
      781 agctgttggt aaaagcggac ctgcgtagcc ttctaaaaca gattgagcaa tcggatgtgt
      841 agaaaactgt tctgcactcg caacatattg catgaatatc tttggatcga tttttgtatc
      901 gatcgtctgg atcgaaaagt cacctttcgt taatgtgccg gttttatcga ataccactgt
      961 ctcagtttgt gcaagcaact ccaagtaatt tgaacctttt actaatacac cgattttact
     1021 tgctcctccg ataccgccaa agaaactaag gggtacggag atcactaatg cacatggaca
     1081 tgagatcact aaaaatgtca atgctcgata gatccattca gaaaacgccg ctccagaaac
     1141 aacaagtgga ggtaaaacag ctaataaaac ggccaatcct acaacgactg gtgtgtaata
     1201 gcgcgcgaat ttagtaatga aattttcagc tggtgctttt ttactgctgg cattttctac
     1261 taattcta
//
LOCUS       DACCWV010000275         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCWV010000275 DACCWV010000000
VERSION     DACCWV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.282"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..864
                     /locus_tag="IVW84_002969"
     CDS             <1..864
                     /locus_tag="IVW84_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP7919086.1"
                     /translation="REKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAE
                     QYRRSLELSKD"
     gene            936..>1264
                     /locus_tag="IVW84_002970"
     CDS             936..>1264
                     /locus_tag="IVW84_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919087.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVN"
BASE COUNT          559 a          146 c          207 g          352 t
ORIGIN      
        1 cgagagaaaa ataatttaaa gaaaaaaatg ctcattccat ttgattcaga agtgaaagga
       61 ttactcaata aactaactat ttctaatata gactcgacta gggaaaaaat cataaaaata
      121 tttgataaaa ttaataattt attcaagaat gacaatgctg aattaaggaa gggtctttta
      181 gagtttaaac ttcaagaact agaacttcat tactcttatt tagtgaaaat taccgaagaa
      241 aaagaacaac aaaaagcaat aaaagaacag atgatagaag aggaaaaagt tcgtagagaa
      301 attgatcgtg aaaagaaacg tattgataaa gaacaacgtc aattcaattc tgaaatttcc
      361 aaattaatcg catatatgca aaaagctaat gcagatgtgg aaaaagaatt atatgcaaat
      421 aaaattcaag aactggaaga aaaattaaaa gaattagaag ttatacgtga aaatgtatta
      481 caaagagaat taaatactag agccggttat gtgtatgtaa ttagtaatat tgggtctttt
      541 ggtgaagaca tatataaaat tggtatgact agaagattag aacctatgga tagaataaaa
      601 gaattgagta gtgcatcggt tccatttgaa tttgacgttc atgcgatgat tttttcagag
      661 gacgctccgt ctttggaaac taaattgcat aatcatttta gaaaacaaga agttaataaa
      721 attaatcaaa gaaaagaatt tttcaaagtt tcactagacg aaatagagaa agttgtttta
      781 gaaaattata atggaacagt aacatttaca aaattagcta aagctgaaca atatagacga
      841 tctttagaat tgtcaaaaga ttaaatatat aaaaagcctt cgggcttttt cttttaacaa
      901 ttaataggaa cgtatattcg aaaggaatag aaataatgtg gttagaaaaa ataaatgaca
      961 aaaaatttaa gtacaccgaa cgttatacgg atcccttaac tgaaaaaaag agaaaagtgt
     1021 ccgttacttt atctagcaat tcacgccaag cttggaatca agctaactta cttttgaatg
     1081 aaaaaatcgc agaaaaaata aaacgaaatg aagaattacc actaactttt ggagaattaa
     1141 aaactaaatg ggatgaaaaa tacaagccaa ctgtaaaaga aagttcctat cgaacaactc
     1201 aggtttatct atcattaatt tcaaaatata taaaagatga tgtacttgta aaaaacgtta
     1261 atag
//
LOCUS       DACCWV010000276         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCWV010000276 DACCWV010000000
VERSION     DACCWV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.283"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1021
                     /locus_tag="IVW84_002971"
     CDS             <1..1021
                     /locus_tag="IVW84_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919088.1"
                     /translation="IFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESKSFDD
                     NIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNN
                     KNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGR
                     ENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCK
                     IADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSG
                     SVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAES
                     LDDIL"
     gene            1018..>1246
                     /locus_tag="IVW84_002972"
     CDS             1018..>1246
                     /locus_tag="IVW84_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7919089.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKN"
BASE COUNT          478 a          145 c          210 g          413 t
ORIGIN      
        1 aattttttcc gatgagtatg ttgaaattat tatttctgat cattctaaaa acactaaaga
       61 aattgcgaaa aaaatagaag aaaaaaaagt gtttgatggc tgtcattatt tagaaagcaa
      121 atcttttgat gataatattg ggtattttca aaagattaag gactgtcttc agatttcttg
      181 tggtaaatca aatcgatatg cctattattt gaaaaactgt aaaagtgaat attttgatga
      241 atttatttgt ttttcaatgg gagaggtttg gttggaagga atatatgctt gcttatatcg
      301 aaataataaa aatgtacagg tatcaatttt agaagaaggt attttaagct atggagttga
      361 cattattcca acagcaagaa caaaagctat cgcagtactc aattacttaa aaaaaaggac
      421 tccactatat aacaaaagct ataattttta ttgttttttt cctaatatat atacaggaag
      481 agagaataca atacaaattc cattagttac agctgggtca agaaccgcat tgcaactttc
      541 ggatattttt aaccctatga tagatattta taaagaaaaa aaatatatat tttttacgtc
      601 tgtttatgat tttgagggcg gagaacctgt cggagaatat gaattagtat gcaaaatagc
      661 agacttagta ggaaaagata atctaattgt taagacgcat cctagagatg taagaacaat
      721 ttataaagat aatggattta tcgaggataa aaattcatca ataccttggg aggcgattca
      781 attgtcaggc gattttaacg ataaaatatt tatgacaatt aattcaggaa gtgttatttc
      841 aggtagtaca atgtctgcaa aacctgtacg cacgtattat atgtataaat tgtgtaacat
      901 agaaggaaat ccgtcttgca aaaagaatgc gtatgatatt gagcgattgc ttaagcaaaa
      961 aacgatgaaa aacattttaa agaatgtgaa aatagcagaa agcttagatg atatactatg
     1021 agcaaaacta aatatttaat taaaaatatg ggaatattaa caataagtaa tttttcttca
     1081 aaaattttag tttttttgtt agtcccttta tatacaagtg tattatccac aacagaatat
     1141 ggcacatatg atttgacaat ttcaacagcc acattattgt ttccaatttt aaccttaaac
     1201 attgtagatg ctgtgatgcg ctttttaatg gataaagata aaaata
//
LOCUS       DACCWV010000277         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCWV010000277 DACCWV010000000
VERSION     DACCWV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.284"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVW84_002973"
     CDS             200..742
                     /locus_tag="IVW84_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919090.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVW84_002974"
     CDS             832..>1246
                     /locus_tag="IVW84_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7919091.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCWV010000278         1242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCWV010000278 DACCWV010000000
VERSION     DACCWV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.285"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            18..491
                     /locus_tag="IVW84_002975"
     CDS             18..491
                     /locus_tag="IVW84_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7919092.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="IVW84_002976"
     CDS             500..727
                     /locus_tag="IVW84_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7919093.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          392 a          196 c          208 g          446 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1201 acagtgatgg acaattcttt tttattttta acattatttt ga
//
LOCUS       DACCWV010000279         1221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCWV010000279 DACCWV010000000
VERSION     DACCWV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.287"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..1008
                     /locus_tag="IVW84_002977"
     CDS             <1..1008
                     /locus_tag="IVW84_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7919094.1"
                     /translation="KLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSG
                     VFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIG
                     IFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLA
                     LNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILIL
                     WLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFIN
                     SEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKI
                     S"
     gene            1018..>1221
                     /locus_tag="IVW84_002978"
     CDS             1018..>1221
                     /locus_tag="IVW84_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7919095.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNED"
BASE COUNT          498 a          112 c          206 g          405 t
ORIGIN      
        1 aaactaaaag atgctaaagt agttagcgga gaacaaagaa taaatagaga agatttatct
       61 gacgaattta agaatgttgt ttcactcgaa gctacaaata atactaaaag aaatatttta
      121 tttagtagtg gagtatttac aattaaagaa ggtaaaaata tagaagaaaa tgataagaat
      181 tcaattattg ttcatgaaga atttgctaaa caaaacaatc taaaattagg tgatgaagtt
      241 gatcttgaat tactagatat tgaaaaaagt ggaaaaataa aaagtcataa atttaaaatt
      301 atagggatct tttctggtaa aaaacaggaa acatatacag gattatcttc tgattttagt
      361 gaaaatatgg tgtttgtaga ttattcaaca agccaagaga tattaaataa ttcagaaaat
      421 aatgaaattg caaataaaat tttaatgtat tctggtagtg cagaatctac agacttagcc
      481 ttaaacaaat tgaaagagct taaaattgat gagtcaaagt attttgttga aaaagattct
      541 aatgcgtttg aagagtcttt agagtcagtg agtggaataa aatatataat aaaagtaatg
      601 acttattcta ttatgttagg tgggatggtt gttctttcat taatcttgat tctatggtta
      661 agggaaagaa tttatgaaat aggtatattc ttatctattg gaacatctaa gatacaaatt
      721 ataatgcaat ttatattcga gttaatattc atatcgatac caagtataat atcttcctta
      781 tttttaggaa atgtattact aaaagtaatt gtagatggat tcattaactc agaggattca
      841 atgatttccg gtggaagttt aataaataat agtagtttta tgtcaaatat aacaacactt
      901 ggacaaagtt atttaatatt aataagtatt attgttttat cagttgtatt tgcttcttca
      961 ttaatgttaa tcaagaagcc taaagaaatc ttatcaaaaa taagttagga gaaaataatg
     1021 gatatattag aaataaagaa tgtagcatat agttatgcaa attctaaaga aaaagttttg
     1081 tcaggagtaa atcaaaaatt tgaacttggg aagttttatg cgatagtagg aaagtcagga
     1141 acaggaaaat ctacacttct ttccttactt gctggacttg ataaacctca aacaggaaaa
     1201 atattgttta agaatgaaga t
//
LOCUS       DACCWV010000280         1184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCWV010000280 DACCWV010000000
VERSION     DACCWV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.288"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..1155)
                     /locus_tag="IVW84_002979"
     CDS             complement(1..1155)
                     /locus_tag="IVW84_002979"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7919096.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          329 a          257 c          220 g          378 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagccgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaa
//
LOCUS       DACCWV010000281         1183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCWV010000281 DACCWV010000000
VERSION     DACCWV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.289"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..563)
                     /locus_tag="IVW84_002980"
     CDS             complement(<1..563)
                     /locus_tag="IVW84_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7919097.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
     gene            complement(574..771)
                     /locus_tag="IVW84_002981"
     CDS             complement(574..771)
                     /locus_tag="IVW84_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7919098.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(794..1087)
                     /locus_tag="IVW84_002982"
     CDS             complement(794..1087)
                     /locus_tag="IVW84_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919099.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
BASE COUNT          326 a          238 c          191 g          428 t
ORIGIN      
        1 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
       61 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      121 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      181 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      241 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      301 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      361 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaatg
      421 tactgattat ttgctacttg tgagctacct tcgtttgggc ctaaattaaa ttcattgtta
      481 atcgtatagg caaacccatt aattggcaat aaaaaaagag ccgttaatag gctcatcgca
      541 gtaatagtaa ttttcttttt catttgtttc ctcctatttt ttcaaattat aagccgacac
      601 gccagtgata acgcctaaaa atgttgctac tgcattgata gtgagtactg tcatatctgt
      661 tccattccat ccatacgctt tgcctagtgt tgcaactaat acagatgcag ctggcaatac
      721 tgttaaaact gtccatttaa tgacttgata atacttatcg ggtaaaatca tttcttctta
      781 actcctttct tttttaccga gatctttctc taaataaagt tttgatttgt tgtgtgtgtt
      841 ctaccaattt ttctgtatgt gtatctaatc tttcatcgtg tttctttagt tcttcatgaa
      901 tcatcaatcg atctgatttg ctcgattcta aatctttagt cagcaaatct aaattgtgac
      961 ttacttttga aagagtttca gtaatcttcg agaaagatgc agtgactggt cttattacta
     1021 ataaaatcaa agaaacaatc gcagtgattg atcctgcgat tgttccccat tctcctaaat
     1081 taatcatgtg acaactcctt taccttaaat aaaaacgcat caattaagat gcgctcttat
     1141 ctttattaat gattttatct gcttcttcgt ctgtaatgca taa
//
LOCUS       DACCWV010000282         1177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCWV010000282 DACCWV010000000
VERSION     DACCWV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.290"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            98..391
                     /locus_tag="IVW84_002983"
     CDS             98..391
                     /locus_tag="IVW84_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919100.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            388..612
                     /locus_tag="IVW84_002984"
     CDS             388..612
                     /locus_tag="IVW84_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7919101.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            609..>1177
                     /locus_tag="IVW84_002985"
     CDS             609..>1177
                     /locus_tag="IVW84_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7919102.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          406 a          180 c          248 g          343 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
      421 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
      481 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
      541 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
      601 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
      661 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
      721 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
      781 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
      841 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
      901 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
      961 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
     1021 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
     1081 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
     1141 acttatcgca aatgggagtt agcaaagaaa agcttgc
//
LOCUS       DACCWV010000283         1164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCWV010000283 DACCWV010000000
VERSION     DACCWV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.291"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..1143)
                     /gene="dnaN"
                     /locus_tag="IVW84_002986"
     CDS             complement(13..1143)
                     /gene="dnaN"
                     /locus_tag="IVW84_002986"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7919103.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT          363 a          217 c          215 g          369 t
ORIGIN      
        1 tgaaaatcat tgttaatttg tacgtacagg agtaatcagc tgaataaatt gaacgccatc
       61 ttccgttggt tccaatgtaa atggacgaat agcagagata aatttcactt tgatgctcat
      121 atctccaaat gcacgcaacg ctgctttcat ataatccgga ttgaaagaaa tatctaatgg
      181 atcgccactg cttgctgtat aactcaagct ttcttctact ttcccgattt ctggcgaatt
      241 tccgtataaa acaaccgcat ctggacgaat agataaacga acgatgttgt tacggccttc
      301 atgagaaagt aaagaagccc gttcgatcgc cgctaaaaag ctaggaacag aaaattcaac
      361 ctctgtatta aaacttgaag gaatcaaacg attggtatca ggatagtttc cttctagcaa
      421 acgggaatag aaatacatcg tttctgtttt gaacaacact tggttttcca taatgctgat
      481 ttcgacgatt tcttcttcat ttgttaatga gcgagataat tcgatcaaac tttttccagg
      541 aataacaata tcaaaatgat cagccgcttg ttctactgga atcacgcgtt gacttagacg
      601 gtgagaatca gtagctacag ccaataaaga attatcagat aaaatgaaat gcacacctgt
      661 caagattgga cgactctcat gttgtgagac agcaaaaact gtttcgttga ttagtttagt
      721 caatacgtgt acaggtaatt tcatctggtt atggctttcg acaacaggaa gatgaggata
      781 gttatctgca tctaatccat ttacgataaa attcgctttt ccagaagtaa tcgctacttg
      841 tttattttct aaaacttcta aagtaaatgt ttcttcagga agtctccgaa tgatttcgct
      901 aaagaaacgt gcttgtaaaa caatggaacc agtagattcg atttgcatat ttgctttttc
      961 gttttcaaca gacaaaaaag tttcaattga tatatcagcg ttgctccccg tcaaagtcaa
     1021 accttcttgt gtcagtgtga tttttacacc tgtcaaaata gggatcgtgg ttttgcttga
     1081 aatagctcgt tgaacagttt gcaattcctg cataaagcta gctcggttta aagtaacttt
     1141 catgatgtat ctcctttgaa tgtt
//
LOCUS       DACCWV010000284         1104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCWV010000284 DACCWV010000000
VERSION     DACCWV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.292"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..147)
                     /locus_tag="IVW84_002987"
     CDS             complement(<1..147)
                     /locus_tag="IVW84_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7919104.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMA"
     gene            complement(144..797)
                     /locus_tag="IVW84_002988"
     CDS             complement(144..797)
                     /locus_tag="IVW84_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7919105.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          358 a          196 c          111 g          439 t
ORIGIN      
        1 tgccatatta tgaatctttt cttttctcgt ctccaaatat gttttaggaa agataaaaaa
       61 aattaatgaa tgaactaata taattattat tccaagaaca gaaaatattt gtatgaacat
      121 ttttggaaat atctttaatt ttttcatttt ctctccaatt tatatcctac atttcttata
      181 gtagttatac agtctaattg taattttttt ctaagttctt ttatatatac atctattact
      241 cgatcataag gagtttcttc gctatctttc catacataat caataatttg cattcttgtt
      301 aacacttgtc cattattatc caataaacat tttaatactt ctagttcttt tgcatttaca
      361 tctatttctt caccatttat tttagctgta tagctattaa aattaaccga tagatcctta
      421 tattcaaact tctctaaatg tccataattc ttcttaatta aagaatctat tctagctttt
      481 aagaccggca atgaaaatgg cttttctaca tatccgtcta ctaaattagt aaatgcatca
      541 attttatatt cttcatcact aaatgcagtc aagattagaa ttggtaaatt gcttttcttt
      601 ctaatttctt tcaacacttc taaaccattt ataaaaggta tctgaatgtc taagataacc
      661 aaatttatat cgctattaaa ttttgacaag gcttctcttc catctttagc ttgaataaca
      721 gtatatccaa attctgaaag atattcactt attccctctc ttatcatatt atcatcttca
      781 acagttaata ttttcatata tacctccact taagcctata cactagatat tatacagcat
      841 attatggctc tataatatct agtgttggtg acaccttctg ttgccaatac tttttctttt
      901 ttctaataaa tgcaaacaga caccttgaaa tcctttagaa taaagggttc tataatattt
      961 tgtgttgttg gaaaatcgaa ggcgattttc tactaacact ttttcttcgt ttcaactcaa
     1021 aaagagactc acttttggta aaatttagtc gaccaaaaca tcaccctacc aaaggagtcg
     1081 ctctatggat aatcatacta gaaa
//
LOCUS       DACCWV010000285         1099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCWV010000285 DACCWV010000000
VERSION     DACCWV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.293"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..954
                     /locus_tag="IVW84_002989"
     CDS             1..954
                     /locus_tag="IVW84_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7919106.1"
                     /translation="MKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIF
                     IFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVI
                     FQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISL
                     ICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALF
                     ALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLD
                     FLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          277 a          218 c          189 g          415 t
ORIGIN      
        1 ttgaaaatta ttccgatccc gatctgcggg ttgatccttg ggttagcttc ccttggaaat
       61 ctgtttaaag actatcacca tgttgcttta ggcaatctaa ctggcggtat ctctatgatt
      121 cttatgatat tcatttttgt taaattgttt atcttattcg aacacacgaa acaaacgttg
      181 catgatccaa tcatcgcctc cgtttcacca acttttacga tgtcactcat ggttatttgt
      241 acttattttg tttcatatga aagtattgca ccaattgtca aattcatttg gctgtgcgct
      301 gttatcttcc aagtgatcct tgtcttttat ttcaattatc accatgttat caaagctgat
      361 ctttcaattg aagcaattta tccaagctgg tttatcattt acgttggatt tggggtcatc
      421 actgtaactg ccgggaactt cttcccactt attggaaaaa ttttcttttg gatttcattg
      481 atctgctatg cgttcttatt gccgatcatc atcaaacgtg tctttttcgt aagaaatatg
      541 gctcatccga cacgtccgct gattacgatc gttgcagcac ctggttctct ttgcctaaca
      601 ggttatctta aaaactttgc tgaacctaat ttttatcttg tcgtagcatt gttcgcttta
      661 tctcaagtat tgtatttcgt tgtgctgtct atgcttccac gtttgttcaa gcttcgtttt
      721 tatccaagct atgcagcctt tacattcccg cttgttatct ctgcaacagc tatatttact
      781 actgttcatt acttcaacag tttaggtatc tatagtagcg tattggattt cttaaaagtt
      841 gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc ttgctcatta cgtacgtttc
      901 ttaattaaag aacatagcaa acatggaaaa tttgtttcta aaaaaatcgc ttaataatcg
      961 attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacggcc aaacaaaaac
     1021 agctgttcat gtttttgtac tggttgggct tattaccgca gatgcaagct tttgaacacc
     1081 gtttaccaag aggtcgtaa
//
LOCUS       DACCWV010000286         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCWV010000286 DACCWV010000000
VERSION     DACCWV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.294"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            117..>1068
                     /locus_tag="IVW84_002990"
     CDS             117..>1068
                     /locus_tag="IVW84_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7919107.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAK"
BASE COUNT          338 a          201 c          242 g          287 t
ORIGIN      
        1 aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa ataagcgcct
       61 ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg ggttatgtga
      121 aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa
      181 gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat ttaagtgaac
      241 tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa
      301 cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa
      361 cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa gctgcgattc
      421 tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca gggaagtcga
      481 tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct gcagatgcgc
      541 catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc tttggacaca
      601 tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc ggtctaatag
      661 ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg ccggaagggc
      721 aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga gaaagtgcac
      781 aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca gaaagtttct
      841 tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt gcttcacgtt
      901 cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg aatcgtttga
      961 atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat gatgcggaag
     1021 gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaat
//
LOCUS       DACCWV010000287         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCWV010000287 DACCWV010000000
VERSION     DACCWV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.295"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..993)
                     /locus_tag="IVW84_002991"
     CDS             complement(37..993)
                     /locus_tag="IVW84_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919108.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACCWV010000288         1059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCWV010000288 DACCWV010000000
VERSION     DACCWV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.296"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            52..>1059
                     /locus_tag="IVW84_002992"
     CDS             52..>1059
                     /locus_tag="IVW84_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7919109.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT          440 a          170 c          186 g          263 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaa
//
LOCUS       DACCWV010000289         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCWV010000289 DACCWV010000000
VERSION     DACCWV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.297"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(60..278)
                     /locus_tag="IVW84_002993"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="IVW84_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="IVW84_002994"
     CDS             complement(544..738)
                     /locus_tag="IVW84_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919110.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          369 a          182 c          154 g          353 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatc
//
LOCUS       DACCWV010000290         1046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCWV010000290 DACCWV010000000
VERSION     DACCWV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.298"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..845)
                     /locus_tag="IVW84_002995"
     CDS             complement(36..845)
                     /locus_tag="IVW84_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7919111.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          318 a          201 c          183 g          344 t
ORIGIN      
        1 gtcacaacct cttttttgtg gaatctaatt ctctattata gtttaagtag tttttccaga
       61 taaacagcta aaccgtcttc ctgattgctc aaaggagtga catcatttgc aatgcctttg
      121 agttggtcag taccgttagc catagctact ccccaacctg cataatccaa caattccaaa
      181 tcattatgct cgtcaccaaa ggcaatgacg tctttttgat cgatagacag gtagttagca
      241 atctcttgca cgccttttgc tttttggatt ccttttgaaa caatctctaa gatggcagta
      301 ggtccacccc atgtccgaac atcgacataa ctgccaaatt gacgtgttaa ttcagcagat
      361 acagctcctg caaaacgttt gtctgttcgg acaagcaaag atgtaggatt tgtcgtcaaa
      421 tttttaggag aaagcaaatt cttctctcct ggacgagaag aagcaaaaat tttttcatca
      481 aagaaatcaa aactgtcaat aaaaaaagta tcacgatttt cagcagcgat aaaatctaaa
      541 tttagctgct ttttttgcga taagatttca aaagcgattt cacgattgat caacgtttct
      601 ctttcaccat cccagtgctg attagggatg tgtactaatg cgccgttaaa attaaccatc
      661 ggtgttgtta attctaattg ttggtaaaac tgatggctca ttcggaatgg gcgtcctgta
      721 gcaatactga catgatgacc ggcattgatt gcttttttta gagtctcttt tgttttagat
      781 gtaatcatcg aatcttgatt gagggtagtt ccgtccagat cgatggcaat taatttttta
      841 ttcaataagg gtccttcctt cctcaactag catttgtttt agtctagcat attttaggta
      901 attaacaaga gcaatcagct attcctaaca aaaactacag ttatggctat ataaatagct
      961 aaacataagc taaacacgaa cgttaagtat tattttcgct ttttttgtta aaaaaacgtt
     1021 gaaaagcgaa tgaaacgtct atataa
//
LOCUS       DACCWV010000291         1022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCWV010000291 DACCWV010000000
VERSION     DACCWV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.299"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            27..>1022
                     /locus_tag="IVW84_002996"
     CDS             27..>1022
                     /locus_tag="IVW84_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7919112.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLL"
BASE COUNT          407 a          101 c          206 g          308 t
ORIGIN      
        1 tataaatatt agaggtataa aaagttatga aacacaaggt aagtgtgata gtaccaattt
       61 acaatgttga aaaatattta agaaaatgtg tggatagttt attgagtcag acattaaaag
      121 agatagaaat cgtattagtt gatgatggtt cgccagatgc ttcgggggaa atagcagatg
      181 agtatcaaaa aaaatattct aatgtaaaaa caatacatag agaaaatggg gggttaggac
      241 cggcaagaaa tacaggaatt gagaatgcga caggagaata tgtggctttt cttgattcag
      301 atgattgggt agaaagtgat atgtatgaaa aattatactt agttgcgtcc aaagaaaatg
      361 ctgatatagt agttagtgga cattgtgatt ttgcgaatga caaagctatg gtgaaaaagc
      421 aacatccttt gagtgggaat gtatatgata cgcctaatag tattaaaaat gtacgaaaaa
      481 atttatttgg acatagtcca aaagatcaag aagtagaggc cttcccgatg tcagtatgta
      541 tgtctttgta taataaaaat ctgctagata aatatagact aagatttgaa aaaattttat
      601 ctgaagatac aatttttaac ttgaatgcat atgatgtcgc aaataaaatt gcttttacag
      661 aatatacaga ttattgttat agaaaagaag aacaagagtc tattacaaaa agtttatctc
      721 caaataagca gacggaattt aaagaatttt taataactct aaaagctata gcatcgaaag
      781 aagatttaga atgtcaaatc agagcaaaac gtatggcaat tgattattgt aggctttatg
      841 tgggcttagt tgatgatgca aatttgggaa ttaaagagaa aagaaagtat ataaaaaaat
      901 ttattgaaga tgagggatta attcaatgtt ggaagggata tcctacaaat acgctaccat
      961 ttcaacaaaa attttttcat aaaatgcttg aatgtaagtg ttatacaata ttgttgttgt
     1021 ta
//
LOCUS       DACCWV010000292         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.300, whole genome shotgun sequence.
ACCESSION   DACCWV010000292 DACCWV010000000
VERSION     DACCWV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.300"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            369..1002
                     /locus_tag="IVW84_002997"
                     /pseudo
     CDS             369..1002
                     /locus_tag="IVW84_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACCWV010000293          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCWV010000293 DACCWV010000000
VERSION     DACCWV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.301"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..511)
                     /locus_tag="IVW84_002998"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IVW84_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IVW84_002999"
     CDS             complement(570..>959)
                     /locus_tag="IVW84_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7919113.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACCWV010000294          954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCWV010000294 DACCWV010000000
VERSION     DACCWV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.302"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..332)
                     /locus_tag="IVW84_003000"
     CDS             complement(<1..332)
                     /locus_tag="IVW84_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7919114.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLP"
     gene            complement(434..787)
                     /gene="tnpB"
                     /locus_tag="IVW84_003001"
     CDS             complement(434..787)
                     /gene="tnpB"
                     /locus_tag="IVW84_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7919115.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          267 a          191 c          172 g          324 t
ORIGIN      
        1 ggaaggagat gttctttctc tgttataggt aagtgtttga ttttgtctgc tttcgttcct
       61 acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg aacacttaca
      121 gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa gagagtctct
      181 ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa tagttggatt
      241 tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg ggcttcttga
      301 agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga atcgaactga
      361 ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac attttttgtg
      421 ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga ggttgatcga
      481 tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga acttcgtctt
      541 tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga agaaaaccat
      601 caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat aaaaaaacgg
      661 catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga ccatcaattc
      721 cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga agtaaatcat
      781 aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt aataccgtta
      841 tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt ggcagccgcg
      901 ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac aatc
//
LOCUS       DACCWV010000295          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCWV010000295 DACCWV010000000
VERSION     DACCWV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.303"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..222)
                     /locus_tag="IVW84_003002"
                     /pseudo
     CDS             complement(1..222)
                     /locus_tag="IVW84_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322179.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(471..671)
                     /locus_tag="IVW84_003003"
     CDS             complement(471..671)
                     /locus_tag="IVW84_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7919116.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          341 a          150 c          153 g          303 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctagtttt cgcagcagga
       61 attttgaaat agttccttat tagaatagta gagattgatt aataagctat cttcgattgc
      121 taaaaagaaa acttctgaat tccatacttt ataaaaatct ttgataaatc tattcgaagg
      181 gtcaaattta aaccataatt gtgtttttcc ttccatcagc ataaaagttc acctcaaaaa
      241 gagtatacga acgaatgttc tttttgtgaa ggcggaattt aatatactga ataatagaaa
      301 acaagtactt gtgccaaatt atgtgccaaa aaaattcgaa ttcaacaaaa tgtaaacaaa
      361 tagaaaacct tctatattaa gatttcctgc aataacaaac acaacagaag acgtgtaata
      421 gttagtcagg aacgtacaaa taacccctgt atcctttgcg gtacaggggg ttattttgta
      481 taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac gctaagtata
      541 tttactcctt gataaagtaa aatagatgca tgcgtatgtc ttagtccatc aataaacagt
      601 cgaagagaaa atagggaaaa attgattact aaagaaaaac ttactaggtc gacggtgtag
      661 ttgattttca tacttcaata gattcgaatt aaacgatttc gattggctta taccagtcct
      721 atccaacgaa agatatatcg ttcttaactc tagcggtata acttgaagct ttacgtaaag
      781 ccaatgggaa tgaactggag atgacttttt attattttta gtaatagctg atgggttaat
      841 acttacaact ttccttttca aagtaccata taaaaaaacg gtggggttgt ttagcgaaat
      901 aaaggatcta agccaatagc ttagatcctt tattctatgc tttatat
//
LOCUS       DACCWV010000296          919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCWV010000296 DACCWV010000000
VERSION     DACCWV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.304"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..869
                     /locus_tag="IVW84_003004"
     CDS             <1..869
                     /locus_tag="IVW84_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7919117.1"
                     /translation="VQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQF
                     CTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHV
                     RRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIA
                     FYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRK
                     NWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLP
                     WNKDVQQHFSRN"
BASE COUNT          296 a          167 c          201 g          255 t
ORIGIN      
        1 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
       61 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      121 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      181 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      241 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      301 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      361 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
      421 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
      481 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
      541 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
      601 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
      661 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
      721 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
      781 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
      841 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
      901 ggtcgggtta tttgacgta
//
LOCUS       DACCWV010000297          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCWV010000297 DACCWV010000000
VERSION     DACCWV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.305"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..>910
                     /locus_tag="IVW84_003005"
     CDS             6..>910
                     /locus_tag="IVW84_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7919118.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNK"
BASE COUNT          313 a          121 c          165 g          311 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga
//
LOCUS       DACCWV010000298          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCWV010000298 DACCWV010000000
VERSION     DACCWV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.306"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..355
                     /locus_tag="IVW84_003006"
     CDS             <1..355
                     /locus_tag="IVW84_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919119.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IVW84_003007"
                     /pseudo
     CDS             615..910
                     /locus_tag="IVW84_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACCWV010000299          907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCWV010000299 DACCWV010000000
VERSION     DACCWV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.307"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>907
                     /locus_tag="IVW84_003008"
     CDS             <1..>907
                     /locus_tag="IVW84_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7919120.1"
                     /translation="NQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLI
                     GFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINS
                     TADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYG
                     NTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGP
                     ILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVG
                     KDIFIKHYHIVVESWGLLFVQAIIEI"
BASE COUNT          287 a          115 c          177 g          328 t
ORIGIN      
        1 gaatcaattt ctaatacaat ttgctaaagg gttagaaaaa gttaaagcta tgggcatagc
       61 aggtattatc agcacaattg taacaataat tgccaatatt ttcttcttgc ttattctgaa
      121 atggggatta attggttttt tcattgcaaa tattttatca cagattattt cagctactta
      181 tctgtcagta ttagtgagaa tatgggaata tatcgatttt gaggctaaag atcctcagct
      241 aaagagagaa atgctaaatt actcgattcc attaattgca actgttgttg gatggtggat
      301 taatagtaca gctgataggt atgtggtttc ctttatagta ggtgtctcgg cgaatggctt
      361 attgtctgtg gcatataaaa tacctcaaat aattaatact ttgcagggaa tatttataca
      421 ggcctggcaa atttctgcaa ttaaagaata tggagataat gagactgcaa aattttatgg
      481 aaatacattt gttatgatta atttgttaat gtgcataacc tgttcaagtc ttatcatatt
      541 aactagaccg ttttcacata ttctttatgc gaaagacttt tatgaagcgt ggaaatatgt
      601 tccattttta ttaatagcta atgttttaaa ttgtgcatca ggtctgttag gacctatctt
      661 atcagcaaaa aaagattcta aatccatgat gtggtctgca atcatagggg cattagttaa
      721 tgtgattatg agtgtagtcc ttgtttataa gataggggtt caaggtgcca caatagccac
      781 tgttatatgt tcatatgtaa tatatgttgt tcgtaaacga gctgtgggaa aagatatttt
      841 tataaagcat tatcatattg ttgtggaatc ttggggatta ttgtttgttc aggcaatcat
      901 agaaata
//
LOCUS       DACCWV010000300          895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCWV010000300 DACCWV010000000
VERSION     DACCWV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.308"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(229..777)
                     /gene="aac(6')"
                     /locus_tag="IVW84_003009"
     CDS             complement(229..777)
                     /gene="aac(6')"
                     /locus_tag="IVW84_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7919121.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          242 a          198 c          134 g          321 t
ORIGIN      
        1 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
       61 tttcttacta ctcgaaacta agcgactttt tcacaacctc tggtacacgg tgttccaaaa
      121 gtagcttctt cggaaataag ttggaatttc acaaaaattc aagaagtaat tttcgtaaat
      181 tccttcttat ttcttgaagc tgattacttc tgtcacaacc tctttttatt attgagaatt
      241 tggtcgagga ataatcgttt ttgccatcca aatatccggt ttgtcccagc catttgcatt
      301 tggtaagaca cctacgattt tataacctaa tttttcatag aattcatacg gatgttcacg
      361 aaggttctgg atggaagcca ctttatcaaa tgtatgctca tacaggtccg tttgacttaa
      421 cgttgttcca tggtctaaat catccgtacc taaataaatc gtgattcctc ctctggaagc
      481 tacttctttt tctaagtaat tgactaatcg agtacctatt tggttctttc gtcgggagct
      541 ttctacaact aatggatgca attcccaacc tgtgataccg tattgaggga ttgcaccaat
      601 aaatcctact aactcatctt ggtctaccgc tgctaccgcg attcgttctg gattcatcat
      661 ttcttctact tcttctgccg agctgtctcc atattcttcc ggccaagtca gtctcagtaa
      721 atcagaaagc tgatctttca atactggatt attacggtca aattcactga ttatcatacg
      781 tcttccccct tgttcttgct tattctaaca gtccttttga aaaaacgaaa tgttctcgct
      841 caaaaatctt tagctaccaa tcagtatctc tattctttca ttatttatct tactt
//
LOCUS       DACCWV010000301          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCWV010000301 DACCWV010000000
VERSION     DACCWV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.309"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            65..598
                     /locus_tag="IVW84_003010"
     CDS             65..598
                     /locus_tag="IVW84_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7919122.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          274 a          163 c          165 g          264 t
ORIGIN      
        1 tgttaaattt atttgctttt ttcaaaagta tgttacagta atttcccaag gaggttttac
       61 agaaatgaat tttgttgcca tggattttga aactgccaat catcagcctt atagtgcttg
      121 ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt gatgaattct atacattgat
      181 acagcctgag actccttttt tctggcgaaa tgtccaaatt cacggaatcc atcaagaaga
      241 cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact atccaaccat attttcaaaa
      301 aaatcgttta gtcgttgcac ataatgctgc ttttgataca aaggtcttag cgggatgctt
      361 agattattat catcttgaac agcctaatta tctttcttta tgtacagtga aaagtagccg
      421 tcgcctgttt cctgaatttc caaatcatcg attgaacact gtgtgcgaaa atttgaacat
      481 ccaattagat catcaccatg atgcattgga agatagtcgt gcatgtgcgg aaatcctgct
      541 ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa cttgtgacta taaagtaaaa
      601 gaatacttat gaaacacaca aagaggtcgt gagcctgccg tttcactccg agtcacaagg
      661 agcaatttca aaaaatagct tctcatgttt attaaatatt gtgactggtg accgaggagc
      721 tggcaggaga acaccgttta ttggagaggt tgtgaaaaag ctgttctgca tcaagtaata
      781 agaacagcca aacaaaaata gcttctcata tttttcattt ggttggactt attaccgcag
      841 atgcaagctt ttggacaccg tttacc
//
LOCUS       DACCWV010000302          842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCWV010000302 DACCWV010000000
VERSION     DACCWV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.310"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..648)
                     /locus_tag="IVW84_003011"
     CDS             complement(<1..648)
                     /locus_tag="IVW84_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7919123.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAF"
     gene            624..797
                     /locus_tag="IVW84_003012"
     CDS             624..797
                     /locus_tag="IVW84_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7919124.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
BASE COUNT          260 a          142 c          178 g          262 t
ORIGIN      
        1 aaaagccagc tttagaatat gataatcgtc atagtttcta ggggtataac cagtgagaga
       61 atggaacttg ttcaatcgat attgcaaggt gtttttatga ataaataatt cttctgagca
      121 atttttgatg cttccgttat ttttactgaa gcatgccaaa gtcttcttga acaaatgtac
      181 ttcttcttct gtcaggcttt tcaaaatacg ttttacatat aatgttcgtt gatctgacgg
      241 gattgctggt aaaacgatcc cgaggtctaa atcttcaaaa aagcggatct cgttcacaga
      301 ttgatggaaa gctatccatt cgcaaacttg tttggcatga aggaaagatt gcggtgtctc
      361 ttgctctgta taacaaagcg acccaattcc aaagtttagc tgtaactgat acattttatt
      421 aaactttgtc tggattttat caagcaatga tagcaatgag gctctggaaa tccgattgac
      481 taggataatg atttgttgtc tatgtacagt gagcaaagcc tgtccgtaat aagagaagac
      541 cctttcgagc aactccaata aaggattgat ccgttcttga cacaattgct gattgtcaga
      601 aagttgtccc acaactacat aatatggaga agaaaaatca agctcaatta agtgagggtg
      661 gtatcgacta tgtacttgat atcatcaatg aaccgatggt actttcttat gcgatggaac
      721 aggtagagcg attgcttgtt tgtgcaggaa aacaagcgat gcggctgata cgattaggtg
      781 agaaaataag aagataaaaa tacacggtcc cttcatggcg accgtgcatt tttggttttt
      841 at
//
LOCUS       DACCWV010000303          829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCWV010000303 DACCWV010000000
VERSION     DACCWV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.311"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>829
                     /locus_tag="IVW84_003013"
     rRNA            <1..>829
                     /locus_tag="IVW84_003013"
                     /product="16S ribosomal RNA"
BASE COUNT          210 a          182 c          254 g          183 t
ORIGIN      
        1 agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag gatgagagta
       61 actgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt gccagcagcc
      121 gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc
      181 ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat tggaaactgg
      241 gagacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata
      301 tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc tgaggctcga
      361 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc
      421 taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc actccgcctg
      481 gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg
      541 agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atcctttgac
      601 cactctagag atagagcttc cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt
      661 cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tattgttagt
      721 tgccatcatt cagttgggca ctctagcaag actgccggtg acaaaccgga ggaaggtggg
      781 gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgct
//
LOCUS       DACCWV010000304          822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCWV010000304 DACCWV010000000
VERSION     DACCWV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.312"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          276 a          134 c          104 g          308 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tc
//
LOCUS       DACCWV010000305          821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCWV010000305 DACCWV010000000
VERSION     DACCWV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.313"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            250..>821
                     /locus_tag="IVW84_003014"
     CDS             250..>821
                     /locus_tag="IVW84_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7919125.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSK"
BASE COUNT          357 a           90 c          124 g          250 t
ORIGIN      
        1 atttaaaaga atgggattgc cagttatttt attagaaaaa atggagttgt tttcaaaaga
       61 agaatgcaaa aaatggatat tgagtcatca aatttaaaaa acacctcgtt ttttaaattt
      121 gatgaagtag aaaaaattaa aaagagtgtt cccgtcagtt tgcagatcgg gggaacactc
      181 aataataaag ttctgagaaa actttatttg aatctattat agcaaataaa atcagaagca
      241 ggtgtgacaa tgaatgaaaa agaagttgaa agcttagttg aaaagcaagg ctattctaat
      301 tctgatatat atgcagaatt atactatcaa tatcctattt ttttaaacgt aaatgaaaaa
      361 tataagaaaa tgaaactaag ttctaagtta gtttatatgt tactaaaaga tagaaataga
      421 ctttcattga aaaataactt gtatgatgag aatcaaaaaa tttttttgat atttgaaatc
      481 aaaaatctcg tagaaatttc gggaatgtca aaaaatacaa tcttatcagc actagatgaa
      541 cttcaaaaag ttaaacttct tgtgaaaaga aaaaatggct tcaataaaag taaaatgcaa
      601 atgttcccaa atttttatta tttgttaaag cctgttctgt gcaaagatga tgtttataaa
      661 attaataaga taacccatca agaaaataga caaaaaaact tgatagttca aaatttgaac
      721 tatggaacaa atcaatattc tgttgatagt tcaaaatttg aactatgcga cacatcaatg
      781 aaaacaagag acaaaaacaa ttttgctgat agttcaaaaa a
//
LOCUS       DACCWV010000306          819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.314, whole genome shotgun sequence.
ACCESSION   DACCWV010000306 DACCWV010000000
VERSION     DACCWV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.314"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            8..81
                     /locus_tag="IVW84_003015"
     tRNA            8..81
                     /locus_tag="IVW84_003015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="IVW84_003016"
     tRNA            121..210
                     /locus_tag="IVW84_003016"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="IVW84_003017"
     tRNA            225..296
                     /locus_tag="IVW84_003017"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="IVW84_003018"
     tRNA            312..384
                     /locus_tag="IVW84_003018"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="IVW84_003019"
     tRNA            394..466
                     /locus_tag="IVW84_003019"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="IVW84_003020"
     tRNA            502..577
                     /locus_tag="IVW84_003020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="IVW84_003021"
     tRNA            579..659
                     /locus_tag="IVW84_003021"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="IVW84_003022"
     tRNA            666..739
                     /locus_tag="IVW84_003022"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
     gene            742..814
                     /locus_tag="IVW84_003023"
     tRNA            742..814
                     /locus_tag="IVW84_003023"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:His,seq:gtg)
BASE COUNT          178 a          188 c          224 g          229 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cgccctttt
//
LOCUS       DACCWV010000307          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.315, whole genome shotgun sequence.
ACCESSION   DACCWV010000307 DACCWV010000000
VERSION     DACCWV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.315"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            22..714
                     /locus_tag="IVW84_003024"
     CDS             22..714
                     /locus_tag="IVW84_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7919126.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          158 g          198 t
ORIGIN      
        1 aaaaaaagga ttttagtaac aattggtcta gcttttttgt tagctggttg taccaacggg
       61 aatgaatcga aaaaagaatc aacaaaagaa acagcgaaag caacagttgc aactactaca
      121 acaactagca cagagagcaa gtcaactgca gcaagtacag aaacagcaga tacctctact
      181 tcgtcagagt cttccaaaac gactgaaaca actcaaacat ctacttcaag ttcttctgta
      241 gaggcttcat cttcctcttc agaaagcagc gaacaagttg aacaaacact gtggaatgca
      301 gacaaatccg cacaattaga ggcatttatg accaaatggg gccaaacaat gggacaacaa
      361 tacaaaagtt atacgaatca aatgagtgtg gacttatatg gtcttaaagt ccctcaagct
      421 attttgaacg gggagtggaa gatggctatt ggtggagtgc ctgtttctgc agaatggtca
      481 gaaagtggga caggtcaagc agactatcaa atcacagccg tctattccga tgcagagaca
      541 gaaccatatc tgaaaaagca cgtctatcta ttcgggtttc agcaaaatca gccaaaagta
      601 ttggtgacac aacaaaatca ggggaatccc gataattatc tttacttcaa cgagacagcg
      661 aataatgaat tgaaaaatgg ctttaatcaa atcgtttatg gacaagcaaa ttaatgagat
      721 aaacaagggg ttgtgacatg tcttttatca caaccccttg ctcagtttat ttgttagtct
      781 tttattttt
//
LOCUS       DACCWV010000308          711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.317, whole genome shotgun sequence.
ACCESSION   DACCWV010000308 DACCWV010000000
VERSION     DACCWV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.317"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..602
                     /locus_tag="IVW84_003025"
     CDS             <1..602
                     /locus_tag="IVW84_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7919127.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          181 g          190 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaacgct
      301 tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct
      361 atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg
      421 gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat
      481 tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag
      541 aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat
      601 aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta
      661 gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa g
//
LOCUS       DACCWV010000309          693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.318, whole genome shotgun sequence.
ACCESSION   DACCWV010000309 DACCWV010000000
VERSION     DACCWV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.318"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          243 a          119 c           82 g          249 t
ORIGIN      
        1 attgacgctc cctccaaatt acttcatcaa aacattgata taatttttat tatatcagta
       61 tttattcttg atacataaat gagaaaataa atactttaaa tctattgact acaagtagtc
      121 attaagtctt cataagatgt acaatatgac attaaaaaaa taaaagctta cgaccatccg
      181 ttaaatctga cactaaataa tggactgctt tcttttgtat tagcaggtta cgttgactat
      241 gaatattcac cagattattt ggagaaacct cttcaacacg agaacaatga tattaatttt
      301 agatttgtct acgagtcaaa aagtaaaaca tggttagttg atcaattaag cacgataaac
      361 acaagcttcc atgaattacc taaacctgaa aatgaagttg agatggtcat taaaatcttc
      421 taaaaaagaa gcgttgaagg ggaaatattc ctttcaacgc tcttttttat atgcttagaa
      481 acataaataa attgtatact ttctttttag cccctattac ttttcacgaa aaatatcttt
      541 ttttcgacgc tagcgtttct ctacttctaa tactgtacac agtttttgta ttattgaaaa
      601 attactcatt atttagcatt aatccacatc gttttatcaa tcgaccttca tactaaacta
      661 ctttaaagtc atcttccttc tcgtttttca tta
//
LOCUS       DACCWV010000310          633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.319, whole genome shotgun sequence.
ACCESSION   DACCWV010000310 DACCWV010000000
VERSION     DACCWV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.319"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            145..576
                     /locus_tag="IVW84_003026"
     CDS             145..576
                     /locus_tag="IVW84_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919128.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          247 a           93 c          128 g          165 t
ORIGIN      
        1 tggttgggtc aatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg
       61 gagccattaa atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt
      121 attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga
      181 ttcgagcaaa tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa
      241 ccaccagttc ctaactggag accaagattt gattttgata aaaaacagtg ggttgagtta
      301 gctactgaag aagagaaaaa agggaatgct gttgatagcg tagatgaatt ggctaatatt
      361 aaggcattat acgaaacgct gaaggcagaa aatgatgaat tgaaacagct aaattctaaa
      421 gcaatgctaa acaatgtagc aatcaagcaa gaaaatgtct tattgaaaga aaagtcagaa
      481 agtttagcgc agttgaattc aaaaacgatg cttgcttctg tacaaaatac caaggaaatc
      541 gaagaaatca aaaaacaatt acaaggtggg aagtaacatg tattcatatg aagatatcaa
      601 actaatgtat gactggggct gtttcactaa cga
//
LOCUS       DACCWV010000311          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.320, whole genome shotgun sequence.
ACCESSION   DACCWV010000311 DACCWV010000000
VERSION     DACCWV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.320"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            46..>630
                     /locus_tag="IVW84_003027"
     CDS             46..>630
                     /locus_tag="IVW84_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919129.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMV"
BASE COUNT          199 a           86 c           89 g          256 t
ORIGIN      
        1 aaataaatga tactatatgt aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa
       61 attttttctg taaaaatatt tatagtttat gcatttgtta gtttattata tttatttcca
      121 gcatttaaaa acatggaaat atttggcttt tatgatacgg catttcacat taatcgtgcg
      181 ttaagtttag aatctatttt tagctcccct attaattttg aaacgtttag atcatatggc
      241 atgcaagtaa ataattttta cccatggtta actctatatc ctttattttt attgatcaag
      301 tttactaatt tagcaatagg ctataacctt tttctttata ttgttacgct cattactcta
      361 tttatatgtc attatgtaat gtatgaaatt accaaaaaac atgttactag ctcctttttc
      421 gctattatat atacaacatc ttcttttagg agtgtagaaa tatttcttcg aggggcaatg
      481 ggggaattac tcgcaatgtc aatacttcct cttattttac tcgggtttat aaagctttac
      541 gactcaaaaa aagaatcttg ggttatgttg gcgattagca tgactttatt aatttacact
      601 catgttcttt ctgtagctat gactatggta
//
LOCUS       DACCWV010000312          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.321, whole genome shotgun sequence.
ACCESSION   DACCWV010000312 DACCWV010000000
VERSION     DACCWV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.321"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..625
                     /locus_tag="IVW84_003028"
                     /pseudo
     CDS             1..625
                     /locus_tag="IVW84_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          214 a          110 c          118 g          183 t
ORIGIN      
        1 atgactaaag tgctgactga agaagtaaat gcatttaaag ttagaactct aaatgataag
       61 tatgtcgcta ttttcatgga tgctacttat attcctttaa aacggcaaac cgtctccaaa
      121 gaagcaatct atattgccat tggtatacga gaagacggca ctaaagaagt actaagttat
      181 gcgattgctc cgactgaatc gacctacgtt tggaatgagt tgttacagga catttactcc
      241 agaggggttc atgatgtctt actgtttatt accgatggct taaaaggcat gaaagatacg
      301 attcaccaga tttatcctaa agctaaatac caacattgct gtgttcacgt ttcccgtaat
      361 attgctcata aagtacgtgt caaagatcga aaagaaatct gtgatgattt taaggctgtt
      421 tatcaagcta gctcaaagga agaggcaaat acctttttag ggagtatgat tgagaagtag
      481 caaaaaactt atcctaaagt gacgcagtca ctgataaaaa atcaagattt attgactttt
      541 tatgagtttc cgcctggtat ccgccgaagc atttactcaa ctaatttaat cgaatctttc
      601 aagaaacaaa tcaagaaata cagcc
//
LOCUS       DACCWV010000313          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.322, whole genome shotgun sequence.
ACCESSION   DACCWV010000313 DACCWV010000000
VERSION     DACCWV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.322"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(180..545)
                     /locus_tag="IVW84_003029"
     CDS             complement(180..545)
                     /locus_tag="IVW84_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919130.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          232 a           78 c          113 g          175 t
ORIGIN      
        1 attcaatatc tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt
       61 ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga
      121 aaaacatata tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt
      181 tatggatgcc gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt
      241 ggaactaagt aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag
      301 aagtaacgcc attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata
      361 ttggcaatga ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga
      421 gataaaaata tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag
      481 gcgaaatgca cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt
      541 atcatctttc atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattg
//
LOCUS       DACCWV010000314          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.323, whole genome shotgun sequence.
ACCESSION   DACCWV010000314 DACCWV010000000
VERSION     DACCWV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.323"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          257 a           69 c           62 g          203 t
ORIGIN      
        1 taagacaagg ctttaggcta gaaaacaata gattatatac tactaaaaaa tacaataagt
       61 actaccaatg tcagatatat ataataatta tgtactacca ttaaaattaa cttatttaat
      121 aagatactac tgtgttaatc tgaaaaaaat tactttaaag tagttacata ctacctgtta
      181 atttagatac taccattaat acttaaatta gatactacct atatttatat taagtactac
      241 cgttctaaga ataagataat aaaaaaatac taccaaattg caattaaata ctacttaaaa
      301 tgtaaaaaat aagaactacc caaattaggt agttcttaat aagagaatat cagataaatt
      361 tgtacaagtc ataaaaaaat taatcttaaa tttttaaata tatatacttg ctaattataa
      421 actactttat ttaggtagta cataattaac agataaaact acatgcttgt agtttgtcta
      481 gctaatgagt tgcatatgat ttatataaag gtgtgcagca taacttagtt gaagttaaga
      541 gaatagaata aatatttttt gttttgctaa taattaaaca ctacctaaat a
//
LOCUS       DACCWV010000315          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.324, whole genome shotgun sequence.
ACCESSION   DACCWV010000315 DACCWV010000000
VERSION     DACCWV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.324"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>587)
                     /locus_tag="IVW84_003030"
     CDS             complement(<1..>587)
                     /locus_tag="IVW84_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7919131.1"
                     /translation="QIINQSKSPYSNCIWVCWWQGLDQSPEIVKACVKSIKRNAGKHT
                     VIILTEDNYRQYVDIPKWVEEKKNKGIITRTNYSDLLRLSLLAKHGGLWLDATFYCTK
                     PIPDEYFDIDIWSIRRPDYAHASVASGYFAGYSLACNQESRWMFAIIRDFFLNYWKNN
                     DMMVDYLMIDYMIVLAQRINHEIAQTFDKIPSNNP"
BASE COUNT          188 a          111 c           72 g          216 t
ORIGIN      
        1 aatggattgt tactaggtat tttatcaaat gtctgtgcta tttcatgatt tattctctgt
       61 gctaaaacaa tcatataatc tatcatcaaa taatccacca tcatatcatt atttttccaa
      121 taatttaaaa aaaagtctct aattatagca aacatccatc tagactcttg attacatgct
      181 aatgaataac ccgcaaaata tcctgatgct acggatgcgt gagcataatc aggcctccta
      241 atagaccaaa tatcgatatc aaaatactca tctggtattg gttttgtaca ataaaatgtg
      301 gcgtctaacc acaaacctcc atgttttgct agcaaagata atcttaataa atcagaataa
      361 tttgttctcg taatgattcc tttgtttttc ttttcttcca cccattttgg tatatcaaca
      421 tattgtctat aattatcttc tgttaaaatt ataacagtat gtttccctgc atttcgtttt
      481 attgatttta cacaagcttt tacaatttcc ggcgattgat ccaatccctg ccaccaacaa
      541 acccaaatgc agttagaata tggtgatttt gattgattaa ttatttg
//
LOCUS       DACCWV010000316          582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.326, whole genome shotgun sequence.
ACCESSION   DACCWV010000316 DACCWV010000000
VERSION     DACCWV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.326"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            18..221
                     /locus_tag="IVW84_003031"
     CDS             18..221
                     /locus_tag="IVW84_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7919132.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            221..571
                     /locus_tag="IVW84_003032"
     CDS             221..571
                     /locus_tag="IVW84_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7919133.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          233 a           62 c          105 g          182 t
ORIGIN      
        1 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
       61 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      121 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      181 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      241 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      301 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      361 aaaatatcct gaaattcttg taaataaatt agttaattat atttacatcg tgggttttga
      421 taataaaata cgctttattg ataatgatga aaaattatta atagaattag gggatatttc
      481 taaaaaagct ggaattaata gtaaatacaa agcaaacttt acagataaat cgcaatttta
      541 tagttattca gaaacaatgc ctcaacgata aaatatagta ta
//
LOCUS       DACCWV010000317          568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.327, whole genome shotgun sequence.
ACCESSION   DACCWV010000317 DACCWV010000000
VERSION     DACCWV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.327"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            115..345
                     /locus_tag="IVW84_003033"
     CDS             115..345
                     /locus_tag="IVW84_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919134.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          208 a           74 c           92 g          194 t
ORIGIN      
        1 attcatatat caatagctaa cttaatatca gtcaaattat gccccttttt tgcccctcaa
       61 atatcgcttt acaacaagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
      121 gaagaattta ttagaaaaaa tatcagtgat gaatatgcag acttttatga tcaaagcagt
      181 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
      241 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
      301 tatatattag aggtagagac taagtttaaa aatagatcgg agtaatggtt atgcttttta
      361 atgagacgca attatggttt aaatttgatc cttcgaatag atttgtcaaa gatttttata
      421 aggtgtggga ttcagaagtt ttctttttag caatcgaaga tagcttatta attaatctct
      481 actattctaa taagaactac tttaaaatcc ctgctgcgaa aactagaatg aagaaagacg
      541 tatatttttt gtttgatatc gtgactga
//
LOCUS       DACCWV010000318          565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.328, whole genome shotgun sequence.
ACCESSION   DACCWV010000318 DACCWV010000000
VERSION     DACCWV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.328"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            97..468
                     /locus_tag="IVW84_003034"
     CDS             97..468
                     /locus_tag="IVW84_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919135.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          172 a           97 c           87 g          209 t
ORIGIN      
        1 tacattagat aacatttgtt acgacacgaa aaagcttcac aaacaaatat cgttatttta
       61 aaatgagggc gaactcacac aattaggagg aatagtatgc catttattgt ttactttttt
      121 ctttcattac tcgtgatcta tattccatta ccaacatttt tcctgctcgt caatcgtcta
      181 gcagagcaac atgatacgat tgctgctctt acgaaacttt gtttacttct atttgtttgt
      241 ctagattact ttacgagcta ttattggcta aaacagacaa gagtaaaaaa gagatattat
      301 ttcttccttg tctttttcaa tctctttgag tttctgatcc attgcttttt acttcatcag
      361 tatcatacga tccatttgtg gatcatctgg gttttcaaag tggtcgtatt attttgtatg
      421 ttcacctttc ctatctcaag aaaatttagg gaggcattgt tttattaaag aagcttctct
      481 gtatcaataa agacacattt aaggaaaaac ttttaaaaat ctaattggga atttgcccgc
      541 ataaataaga ggctgtgaca aaagt
//
LOCUS       DACCWV010000319          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.329, whole genome shotgun sequence.
ACCESSION   DACCWV010000319 DACCWV010000000
VERSION     DACCWV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.329"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(156..455)
                     /locus_tag="IVW84_003035"
     CDS             complement(156..455)
                     /locus_tag="IVW84_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7919136.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          178 a          101 c           67 g          209 t
ORIGIN      
        1 gtttttgtgt taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag
       61 aataaaaaaa tacagactct gttcaaataa atgaacctag cctgcattat gagattgact
      121 atacttttta attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt
      181 gccgaagcca ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata
      241 taaaacggtg atactggttt agacaattct cccctgattg ctaacggacg taaactacgt
      301 tccaaattat ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct
      361 atcaaaagta tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt
      421 agtaccatgt tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt
      481 tcttcccact acattctata aacaaagtat acgctacatc tagatttttt taaatcatta
      541 ctggattgtt aaaac
//
LOCUS       DACCWV010000320          551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.330, whole genome shotgun sequence.
ACCESSION   DACCWV010000320 DACCWV010000000
VERSION     DACCWV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.330"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            127..525
                     /gene="spxA"
                     /locus_tag="IVW84_003036"
     CDS             127..525
                     /gene="spxA"
                     /locus_tag="IVW84_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7919137.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           86 c          110 g          159 t
ORIGIN      
        1 ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag
       61 atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg
      121 tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg
      181 tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac
      241 agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg
      301 acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa
      361 ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat
      421 aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta
      481 cgacaattag aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa
      541 cttggaaaag a
//
LOCUS       DACCWV010000321          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.331, whole genome shotgun sequence.
ACCESSION   DACCWV010000321 DACCWV010000000
VERSION     DACCWV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.331"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          185 a           79 c           78 g          202 t
ORIGIN      
        1 cttattgctt ttgcaaaatt tatttacctg ttttctggat caacgatagt gtacattatg
       61 ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa cttaatccat
      121 agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc accccaaacc
      181 acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac taagaagcat
      241 accatatatt tgaatttact aagaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttataatt aggatttaga gtaattaatt agatgtttat tttataggac tgatactata
      361 gattacaaat agtatcgtat ttcgcaatct taaaattatc ttcttactgt gaataagata
      421 ataaaatttg tatttgtcct ttttaggtaa cttattttaa tctatatacc tttctggatc
      481 aacgaaagta tactttatat agtcataacg ccgatggtaa ctatgcactg taacgataat
      541 gctg
//
LOCUS       DACCWV010000322          539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.332, whole genome shotgun sequence.
ACCESSION   DACCWV010000322 DACCWV010000000
VERSION     DACCWV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.332"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            47..331
                     /locus_tag="IVW84_003037"
     CDS             47..331
                     /locus_tag="IVW84_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919138.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            334..>539
                     /locus_tag="IVW84_003038"
     CDS             334..>539
                     /locus_tag="IVW84_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919139.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISI"
BASE COUNT          211 a           93 c           82 g          153 t
ORIGIN      
        1 tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg acattttagt
       61 tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa
      121 taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt
      181 tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat
      241 tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat
      301 cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat
      361 gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat
      421 atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac
      481 acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcat
//
LOCUS       DACCWV010000323          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.333, whole genome shotgun sequence.
ACCESSION   DACCWV010000323 DACCWV010000000
VERSION     DACCWV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.333"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            68..>528
                     /locus_tag="IVW84_003039"
     CDS             68..>528
                     /locus_tag="IVW84_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7919140.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          198 a           73 c          106 g          151 t
ORIGIN      
        1 agtaactaaa aataaatatg ttataatagt atcaaatgaa tattgctaaa agaaaagagg
       61 taggaacgtg gtagcgaaga aatatgtaat cggtaatttt aaaggtggcg taggtaaaag
      121 tacgtgcgcg caaatgttag ggtttgaatc agcagttaat gaaggaaaaa gaaccttgat
      181 aattgacttg gatatgcaag gaaatacttc tgatgtgatg aacttaacgc atatgaactt
      241 cactaacgaa gaaggaggcg gagaaggtga accgattgtt tacgaaaata ctgtgactga
      301 cgtgttgata aacaatcttc cacctaaaga ggctgtctat aaagtaatca ataacctcta
      361 tatacttcca gcagacatgt cctttgaaat gtatgatgat tggatcaaag accaatataa
      421 tgattcatta tcccaattca aatatatgaa agaaaagcta gaaccattat ttgatgagtt
      481 tgacgtcatc tatttagatg tcccaccttc tatttctgta tatagtaa
//
LOCUS       DACCWV010000324          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.334, whole genome shotgun sequence.
ACCESSION   DACCWV010000324 DACCWV010000000
VERSION     DACCWV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.334"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..354
                     /locus_tag="IVW84_003040"
     CDS             <1..354
                     /locus_tag="IVW84_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7919141.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DACCWV010000325          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.335, whole genome shotgun sequence.
ACCESSION   DACCWV010000325 DACCWV010000000
VERSION     DACCWV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.335"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            32..505
                     /locus_tag="IVW84_003041"
     CDS             32..505
                     /locus_tag="IVW84_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7919142.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT          178 a           75 c          117 g          143 t
ORIGIN      
        1 gtaattatta taaattgttg gggttgagta aatggtcatg aatgctattt cggcgatgaa
       61 aaaaacaaaa cagcagctac atgaagcggg gtttaagctg acaccgcaaa gagaagctac
      121 tctgcttgtg cttttggaaa atgagaaaga gcatttatct gcagaagaaa tcttcttttt
      181 agtgaaaaaa aagagtccgg aaattggact tgctactgtt tatcgaaccc ttgaaatctt
      241 gacagaattg aaaattgtcg ataaagtaag ttttaatgat ggattagccc gttatgattt
      301 gagaaaagaa ggcgccaaac atttccatca tcatttacta tgcttgaact gcggcacgat
      361 cgaggaagta gaagaggatc ttttacttga agtggagcaa gtagtagaag aacgttatca
      421 ttttttagta aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat gctatgaaaa
      481 agaaaaaaga aaccagtccg tctgagtgtc tag
//
LOCUS       DACCWV010000326          499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.336, whole genome shotgun sequence.
ACCESSION   DACCWV010000326 DACCWV010000000
VERSION     DACCWV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.336"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..273
                     /locus_tag="IVW84_003042"
                     /pseudo
     CDS             1..273
                     /locus_tag="IVW84_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          186 a           73 c           88 g          152 t
ORIGIN      
        1 gttaaatcta tattttgata tacaaattac agcttcagat actaagttgc ataaaccttt
       61 tccggacccc atacaaaagg caattgatga attgggcata aaaaataaga aacgatttat
      121 attggtgatt cattatatga catgcaaagt gcgaaagctt gtgggatagc atttggttta
      181 gctaaatggg gagccaaaaa tatagaaaat tttcaaagtg tagatataat tattgattct
      241 cctcaagaaa tacttgattt gttagaaaaa tagacaaaga gcgtgagata aggatagggt
      301 ctcacgctct ttgtttttcg ccatttaaag attgaaattt ttgccatcgc cgattgtaaa
      361 attaagctag acaactaaaa aagtcatttg tgctacactc aaaatagttc cgaccaaaga
      421 attataagga gtgagtacaa atgacctata cccatcttac aacggatgaa cttgtaataa
      481 tagagtctta tttcaaaat
//
LOCUS       DACCWV010000327          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.337, whole genome shotgun sequence.
ACCESSION   DACCWV010000327 DACCWV010000000
VERSION     DACCWV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.337"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..413)
                     /locus_tag="IVW84_003043"
     CDS             complement(<1..413)
                     /locus_tag="IVW84_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919143.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
       61 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
      121 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
      181 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
      241 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
      301 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
      361 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
      421 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
      481 aaaacata
//
LOCUS       DACCWV010000328          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.338, whole genome shotgun sequence.
ACCESSION   DACCWV010000328 DACCWV010000000
VERSION     DACCWV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.338"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          162 a           96 c           57 g          173 t
ORIGIN      
        1 agaatgctaa atcactctac aataacggcg aaagcacaaa aaataaaaaa attattacgt
       61 tattaaaaaa acagatgaaa gatcaggttt tattagatct cattctttca tcacttgaag
      121 ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt ccttcaaaca
      181 gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa aatttagatg
      241 tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac agaccaatcg
      301 aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt ttcaagacat
      361 ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg tattttcctc
      421 taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca gcccattcaa
      481 cgaccaat
//
LOCUS       DACCWV010000329          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.339, whole genome shotgun sequence.
ACCESSION   DACCWV010000329 DACCWV010000000
VERSION     DACCWV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.339"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..476
                     /locus_tag="IVW84_003044"
     CDS             6..476
                     /locus_tag="IVW84_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7919144.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          174 a           93 c           73 g          147 t
ORIGIN      
        1 actttatgtc tatttataat tttgaaacta ctttagaaga cggcactgtc tattcattga
       61 aaaaatatgc aggcaaaccg ataatcattg ttaacactgc aacaaaatgc ggtctagcac
      121 ctcaatttaa acaattagaa gaactttacc aagcttatca gacacaaggt ctggttatct
      181 taggctttcc ttccaatcaa tttaaacaag agctagattc agcgactgaa gcagcagaag
      241 cttgtaaaac cacttatggc gttaccttcc ctatgcatca attgattgac gtaaatggag
      301 aaaatacaga tcctcttttt acttatctta aaaatgaagc tcctggtact gtcactaaag
      361 caataaaatg gaattttact aaatttttga ttgatagaaa tggcaaagta atcgaacgat
      421 ttgcaccaca aacttctcca ttgaaaatga ccgctgcaat tgaatccgtt ctttaactta
      481 ataaaaa
//
LOCUS       DACCWV010000330          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.340, whole genome shotgun sequence.
ACCESSION   DACCWV010000330 DACCWV010000000
VERSION     DACCWV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.340"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            122..>485
                     /locus_tag="IVW84_003045"
     CDS             122..>485
                     /locus_tag="IVW84_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP7919145.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKI"
BASE COUNT          188 a           57 c           82 g          158 t
ORIGIN      
        1 tgtcctttcc tattcttaaa atgatgtttg tacttactta aattaggtat aattagtata
       61 tgttttgtga gaaatttaga cttacaataa gcagtgaatt tataatcaac gtataggaga
      121 catggatgaa atgaaactaa ttggtataga tttagacggt actcttttga attctgacca
      181 gagcattagt caaaaaaatg caagaacaat gaaacattta ccggaagact gttttccatt
      241 catttgttct ggaagagaag tagaagacat taataatatt ctcaaaaaat ctggtttaag
      301 gataccagca gtagggttaa atggttctgt tggatttgat ggagaaaaaa agatatttga
      361 gtcttccttc gataaaaatg atgtaaaaaa aattgattct gtcctatcga aatttccaac
      421 caaaatatat acaaatctcg gaagttatga atctcagaat tataaaaata aaatgaaaaa
      481 aattt
//
LOCUS       DACCWV010000331          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.341, whole genome shotgun sequence.
ACCESSION   DACCWV010000331 DACCWV010000000
VERSION     DACCWV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.341"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>473
                     /locus_tag="IVW84_003046"
     CDS             <1..>473
                     /locus_tag="IVW84_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7919146.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DACCWV010000332          471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.342, whole genome shotgun sequence.
ACCESSION   DACCWV010000332 DACCWV010000000
VERSION     DACCWV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.342"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>471)
                     /locus_tag="IVW84_003047"
     CDS             complement(<1..>471)
                     /locus_tag="IVW84_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7919147.1"
                     /translation="HDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGP
                     DLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSD"
BASE COUNT          151 a           69 c           64 g          187 t
ORIGIN      
        1 atccgattta acttttctta caaataattt ttcactttct tttatttgat ctatatccct
       61 ttcatcaaac catttgttca tggaaggatc aataatatga atatttgcta atttcgaagt
      121 ttgtaaagta gtttcttcaa tatttttcaa ttcaagtggt aaatctggtc ctccaataac
      181 atttttattt ttgaacgagg aattaaaaaa aagtgtatga aaaaattttt catctggggc
      241 aaacatacct attttaaaat tcaggtagtc ttgttgattt tgttcataca tttgtattaa
      301 ttctgttagg cattctttcg acaaagccca ccattgagaa ccatgataca aatcaaattt
      361 atggtgtcta atttttgggg gaagtttaat catcctcaga caacgattaa ttatagctct
      421 aaaaatttta aatgcagttg tgttattcct attaattcta ggataatcat g
//
LOCUS       DACCWV010000333          469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.343, whole genome shotgun sequence.
ACCESSION   DACCWV010000333 DACCWV010000000
VERSION     DACCWV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.343"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>469
                     /locus_tag="IVW84_003048"
     CDS             <1..>469
                     /locus_tag="IVW84_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919148.1"
                     /translation="IFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEP"
BASE COUNT          188 a           73 c          109 g           99 t
ORIGIN      
        1 atcttcaccg caggggaagg aacaagtatt gaaagtggaa caacggtctt tgcagtgaaa
       61 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      121 gatgcgaaat ggccagaaga agcaactcaa ccaattacag cagatgatac agaatttgta
      181 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      241 aaagtgacta cagaattgaa taaggaacca gacgcatctg agggaattaa gaacaaagaa
      301 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      361 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      421 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaa
//
LOCUS       DACCWV010000334          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.344, whole genome shotgun sequence.
ACCESSION   DACCWV010000334 DACCWV010000000
VERSION     DACCWV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.344"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>468
                     /locus_tag="IVW84_003049"
     CDS             <1..>468
                     /locus_tag="IVW84_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7919149.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACCWV010000335          463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.345, whole genome shotgun sequence.
ACCESSION   DACCWV010000335 DACCWV010000000
VERSION     DACCWV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.345"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            126..>463
                     /locus_tag="IVW84_003050"
     CDS             126..>463
                     /locus_tag="IVW84_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7919150.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFS"
BASE COUNT          174 a           79 c           83 g          127 t
ORIGIN      
        1 cccgcactat gccattacat ttatatctac gcgaattgta aaattaagtt agaaaaataa
       61 aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac
      121 cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa tcatattatc
      181 ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata caaactattt
      241 ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg caccagtata
      301 aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat gaagtagatt
      361 acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg cgaaaagaaa
      421 ggtctgtttc atgcggtatg agaacacttt atcgtttatt ttc
//
LOCUS       DACCWV010000336          434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.346, whole genome shotgun sequence.
ACCESSION   DACCWV010000336 DACCWV010000000
VERSION     DACCWV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.346"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            33..227
                     /locus_tag="IVW84_003051"
     CDS             33..227
                     /locus_tag="IVW84_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919151.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          186 a           58 c           80 g          110 t
ORIGIN      
        1 taactaaaaa cagcaagaaa gaagaggatc aaatgtatta tgtagaagta aaaacaaaag
       61 gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga atatccttta cttggttcat
      121 ggaaagaagc tgctcctttt tccaaaccat gtgccataaa aataaagaac gagctagaaa
      181 aagaactgac ttgtggtaaa gctgttgtag atatcatcga aaagtaaatc caatgtatag
      241 aaaggaagat cacacatgaa aagcgacgta caaattgcta agattataga attagaagaa
      301 gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg tataccatat gattatttca
      361 atatgtatcc tggaattgga gatctcgtag agctatatca agatgaatca aatatacttg
      421 tcattcatca tgaa
//
LOCUS       DACCWV010000337          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.347, whole genome shotgun sequence.
ACCESSION   DACCWV010000337 DACCWV010000000
VERSION     DACCWV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.347"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..171)
                     /locus_tag="IVW84_003052"
     CDS             complement(<1..171)
                     /locus_tag="IVW84_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7919152.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISI"
     gene            complement(190..345)
                     /locus_tag="IVW84_003053"
                     /pseudo
     CDS             complement(190..345)
                     /locus_tag="IVW84_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT          143 a           76 c           46 g          158 t
ORIGIN      
        1 tattgatatt gaagaattag aactttcata taaagccttt tctatttcat tgcttgattt
       61 cattattgtt aaacaagaat acaagcaaga aagaactata gttaatatga taaaaattat
      121 taaagttcta tttttctttc ttgtaatata tgctatagca ttttttatca ctttcctaac
      181 tactcctctc tattttctta ctccataact aattgcatcc actggacaag catttttaca
      241 tcgtccacat ctgatacact ccagatgatt gcaattctca actgaatctt tattcatttg
      301 gcaaactttt ttacattttc cacaattaat acacttatcc ttatcctgaa tcggctacac
      361 tagactagac agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc
      421 atg
//
LOCUS       DACCWV010000338          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.348, whole genome shotgun sequence.
ACCESSION   DACCWV010000338 DACCWV010000000
VERSION     DACCWV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.348"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          133 a           52 c           59 g          179 t
ORIGIN      
        1 ttatttagac caacagggta taccctgttg gtctttttgt gcgttcatga tatacttggt
       61 gtcattttta tatttaaatg gcacaaatat ttctaattat ttgtcattta acgctgaaaa
      121 tttttgctat ttatctaaaa aagtgacagg atactcattt ggccttcata gaattttcta
      181 aatatgaatc atcaatgaat ttaattaaaa aaataagtag ggcaaaatat aaatgttttt
      241 gccttacttg tttccatcag tttagtttta aattgataag ttatatagtt atggttgaat
      301 gtgtgtctcg catattagta tttttaccat ctgctagttt tattattaat ctataacaat
      361 agataattta taattcgcat attcattaag ctgttccaaa aagctgttca ttgatttttt
      421 tga
//
LOCUS       DACCWV010000339          416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.349, whole genome shotgun sequence.
ACCESSION   DACCWV010000339 DACCWV010000000
VERSION     DACCWV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.349"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVW84_003054"
     CDS             complement(<1..132)
                     /locus_tag="IVW84_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7919153.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          142 a           82 c           81 g          111 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctctcc atacatccat tgtact
//
LOCUS       DACCWV010000340          407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.350, whole genome shotgun sequence.
ACCESSION   DACCWV010000340 DACCWV010000000
VERSION     DACCWV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.350"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            34..>407
                     /locus_tag="IVW84_003055"
     CDS             34..>407
                     /locus_tag="IVW84_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7919154.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTE"
BASE COUNT          167 a           52 c           58 g          130 t
ORIGIN      
        1 aatgatttta ataaaatgat tggagagaaa atgatggatc aaaaagcgtt tatcgaatct
       61 attgattcta caatcgatag attaaaaaaa gaaattcgat tttataatag agtaattggt
      121 atcggaaata ttataaaaat agtcttatca gctagtatcc caatattaat tgatcaggct
      181 tccgaacaca ggtctttact attgattgtt tcaattgctt cagcaataat tacaattata
      241 caaagtggta tgtctgcatt taattatcaa gataaagtac agacttcgac agagttatta
      301 atgaaaattg aaaaagaaaa gttgttatat ataacgaaaa catccccata taataaaaca
      361 gatgaagaaa atttccattt aatcgttaca acccttcaaa ctgaatt
//
LOCUS       DACCWV010000341          398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.351, whole genome shotgun sequence.
ACCESSION   DACCWV010000341 DACCWV010000000
VERSION     DACCWV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.351"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          136 a           59 c           48 g          155 t
ORIGIN      
        1 taaaaaatga taaacctctc tcgaacggtt ttttatcaaa aatcgtcatt tttccatcaa
       61 aatctgctta tgcaaaatta taaaaaaatt ttgcagtttt tcttactttt tttgtcgttt
      121 aattgaatga taaaaaccag tttctttatt cagttaatca aatatgaacg aattagatat
      181 gaatcattgt atttgccgtt gcggaaggat tttttaatta accacagata aacattgata
      241 acgattcgct tattgtaatc aacagattaa atttcatatg ttcatatgta cggatacatt
      301 ttcggaccga ttaattttaa ccattctatt tatttgctta cctattagat atcaataaaa
      361 aactgcttaa cagctatttc agctgttaag cagtttta
//
LOCUS       DACCWV010000342          395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.352, whole genome shotgun sequence.
ACCESSION   DACCWV010000342 DACCWV010000000
VERSION     DACCWV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.352"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..395
                     /locus_tag="IVW84_003056"
                     /pseudo
     CDS             1..395
                     /locus_tag="IVW84_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          123 a           83 c           67 g          122 t
ORIGIN      
        1 ccatacttaa tgcgacgaca atggatttat tcttagcaca atcatcactc ctagaaatat
       61 tcatgacagt caagtcgcct ttcagctcat caaacaatcg aagagacttt ttcctgaaat
      121 caattgtgtt gtcgctgatg ctgagtataa gacacctaag tttgttcact ttttgactca
      181 tttaaacctt cgaccttgtt tgccttattc aaggcccaaa gggaaaaagg ggctattatc
      241 aaaaaatgag tttctctatg acgagtattt tgattgttat atttgtccgc aagatcaaat
      301 gctcgctttt tcaacggtta ctcgagaaag gtaccgagaa tacaaatcca atccaaaaga
      361 atgtgtgaac tgtcctctgc taaaccaatg tacca
//
LOCUS       DACCWV010000343          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.353, whole genome shotgun sequence.
ACCESSION   DACCWV010000343 DACCWV010000000
VERSION     DACCWV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.353"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          141 a           40 c           67 g          139 t
ORIGIN      
        1 tatttaaaag ataactttta agttatcttt aagtaatgct taaagctatg ttatactcta
       61 ttaggaaata taagaaggag tataaaaatg aacaaacata atagaaaaat aacgaataac
      121 aaagaactga tcttgttact aatagggatt tggggtatct ttttactctt taatatgtat
      181 atgccgacta tgagagcgga tgacgtagtt tatgcaagta gattagatga acttggctat
      241 ttgggtgctt cgatagaaca ttataagaca tggagttcaa gaattattat agaactattc
      301 ttaatgttct tttctaaaca ctttatgcta tggaaattat taaattctac gattatgtta
      361 ggaattgtag tattactttg caagtat
//
LOCUS       DACCWV010000344          349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.354, whole genome shotgun sequence.
ACCESSION   DACCWV010000344 DACCWV010000000
VERSION     DACCWV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.354"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..>349)
                     /locus_tag="IVW84_003057"
     CDS             complement(44..>349)
                     /locus_tag="IVW84_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7919155.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DACCWV010000345          348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.355, whole genome shotgun sequence.
ACCESSION   DACCWV010000345 DACCWV010000000
VERSION     DACCWV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.355"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            143..>348
                     /locus_tag="IVW84_003058"
     CDS             143..>348
                     /locus_tag="IVW84_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919156.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKY"
BASE COUNT          153 a           45 c           49 g          101 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatga
//
LOCUS       DACCWV010000346          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.356, whole genome shotgun sequence.
ACCESSION   DACCWV010000346 DACCWV010000000
VERSION     DACCWV010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.356"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>321)
                     /locus_tag="IVW84_003059"
     CDS             complement(<1..>321)
                     /locus_tag="IVW84_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7919157.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACCWV010000347          312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.357, whole genome shotgun sequence.
ACCESSION   DACCWV010000347 DACCWV010000000
VERSION     DACCWV010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.357"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>312
                     /locus_tag="IVW84_003060"
     CDS             <1..>312
                     /locus_tag="IVW84_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP7919158.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     A"
BASE COUNT          108 a           49 c           63 g           92 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc ta
//
LOCUS       DACCWV010000348          292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.358, whole genome shotgun sequence.
ACCESSION   DACCWV010000348 DACCWV010000000
VERSION     DACCWV010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.358"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..185
                     /locus_tag="IVW84_003061"
     CDS             <1..185
                     /locus_tag="IVW84_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7919159.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DACCWV010000349          278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.359, whole genome shotgun sequence.
ACCESSION   DACCWV010000349 DACCWV010000000
VERSION     DACCWV010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.359"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(22..267)
                     /locus_tag="IVW84_003062"
     CDS             complement(22..267)
                     /locus_tag="IVW84_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919160.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT           77 a           60 c           52 g           89 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacct
//
LOCUS       DACCWV010000350          276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.360, whole genome shotgun sequence.
ACCESSION   DACCWV010000350 DACCWV010000000
VERSION     DACCWV010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.360"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          114 a           30 c           50 g           82 t
ORIGIN      
        1 gattccttta aggtgtcact aaaaattata aaaaatagtg ttgacgcact tttgatgtca
       61 tgttataata acgaagtcgt ttggatgaga tagctaataa agcttgattt tatcgaaaaa
      121 aacttcgaaa aagttattga caaataacaa taaagatggt atgataatca agtcgctgct
      181 aatgagtggc aaagacgttt agaaaaaata aacaaagaag ttgttgacat tctcttctga
      241 atctgataag attaaagagt tgttaaaaca aaaaga
//
LOCUS       DACCWV010000351          271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.361, whole genome shotgun sequence.
ACCESSION   DACCWV010000351 DACCWV010000000
VERSION     DACCWV010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.361"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>271
                     /locus_tag="IVW84_003063"
     CDS             <1..>271
                     /locus_tag="IVW84_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7919161.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD"
BASE COUNT          116 a           27 c           45 g           83 t
ORIGIN      
        1 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
       61 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      121 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      181 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      241 gagcattact ttgataattt caaagtagat a
//
LOCUS       DACCWV010000352          270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.362, whole genome shotgun sequence.
ACCESSION   DACCWV010000352 DACCWV010000000
VERSION     DACCWV010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.362"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>270
                     /locus_tag="IVW84_003064"
     CDS             <1..>270
                     /locus_tag="IVW84_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7919162.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DACCWV010000353          266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.363, whole genome shotgun sequence.
ACCESSION   DACCWV010000353 DACCWV010000000
VERSION     DACCWV010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.363"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(34..>266)
                     /locus_tag="IVW84_003065"
     CDS             complement(34..>266)
                     /locus_tag="IVW84_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7919163.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DACCWV010000354          265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.364, whole genome shotgun sequence.
ACCESSION   DACCWV010000354 DACCWV010000000
VERSION     DACCWV010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.364"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            16..222
                     /locus_tag="IVW84_003066"
     CDS             16..222
                     /locus_tag="IVW84_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7919164.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT           90 a           28 c           57 g           90 t
ORIGIN      
        1 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
       61 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      121 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      181 acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt tgtcgttctt
      241 aagaaggacg gcttcttttt tatcg
//
LOCUS       DACCWV010000355          264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.365, whole genome shotgun sequence.
ACCESSION   DACCWV010000355 DACCWV010000000
VERSION     DACCWV010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.365"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>264
                     /locus_tag="IVW84_003067"
     CDS             <1..>264
                     /locus_tag="IVW84_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP7919165.1"
                     /translation="AVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDEL
                     EDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKG"
BASE COUNT          105 a           43 c           60 g           56 t
ORIGIN      
        1 agcagttaaa gcaggttttg taacagagga taatttgtat ctggattcta cccacatcaa
       61 agctaacgcc aataaacata agtttacgaa agaaatgacc catgatgaag cgaaagccta
      121 tcaggatgaa ttagaagatg agatcaatcg tcaacgaata gaggctggaa aacgcccttt
      181 tactttggac ttagaaaagg aagtgaagct gaaagaaaga aaaataagta aagctgatcc
      241 ggaaagcggc tactacgtca aagg
//
LOCUS       DACCWV010000356          261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.366, whole genome shotgun sequence.
ACCESSION   DACCWV010000356 DACCWV010000000
VERSION     DACCWV010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.366"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>261
                     /locus_tag="IVW84_003068"
     CDS             <1..>261
                     /locus_tag="IVW84_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7919166.1"
                     /translation="DIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKA
                     IVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHG"
BASE COUNT           85 a           48 c           55 g           73 t
ORIGIN      
        1 gatatcggac ggacaaaagt acgagaagaa attcgtttcc atcaggctat gttggattgt
       61 ctcacccatt atcaacatac ctattgttgt aaaagctgcg aaaaagaagg actttcttct
      121 tttaaaaaag cgatcgttcc taaacctttg atttctaata gcttaggttc caatagttta
      181 gtggcggaaa cgatccggat gaaatttggt cagaaagttc ctgcctatcg gcaagagaac
      241 tattggaaac aaacacatgg g
//
LOCUS       DACCWV010000357          260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.367, whole genome shotgun sequence.
ACCESSION   DACCWV010000357 DACCWV010000000
VERSION     DACCWV010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.367"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT          108 a           34 c           34 g           84 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt
//
LOCUS       DACCWV010000358          242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.370, whole genome shotgun sequence.
ACCESSION   DACCWV010000358 DACCWV010000000
VERSION     DACCWV010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.370"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT           79 a           49 c           41 g           73 t
ORIGIN      
        1 aaatttatgc aaagttgtga ggaaccattt tcaaaaaaat ttcttacgac tcgaaagtag
       61 gcgacttttt cacaacttct ccactaaacg gtgttcaatc agctgaccct cggtattagc
      121 tcaaaaaata gcaaaatatg aggaactatt ttgccatttg tttctctaat actcaggtca
      181 ggacgctgat ttcacaactt ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata
      241 ag
//
LOCUS       DACCWV010000359          229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.372, whole genome shotgun sequence.
ACCESSION   DACCWV010000359 DACCWV010000000
VERSION     DACCWV010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.372"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>229
                     /locus_tag="IVW84_003069"
     CDS             <1..>229
                     /locus_tag="IVW84_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7919167.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRKD"
BASE COUNT           82 a           42 c           41 g           64 t
ORIGIN      
        1 cttgaaacca gaaggccgta aggcagttga tattgaaaat tcgattaatg aatggcttca
       61 atccgtaccc aaaaatcttt tcaaatcaat tacctttgat tgtggaaaag aattctctaa
      121 ttggaaaagt attagtaata cgaatgatat tgatatttat ttcgcagacc caggaacgcc
      181 ttcccaacgg ggattaaatg aacattcaaa cggactcctc cgaaaagat
//
LOCUS       DACCWV010000360          228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.373, whole genome shotgun sequence.
ACCESSION   DACCWV010000360 DACCWV010000000
VERSION     DACCWV010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.373"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
BASE COUNT           75 a           35 c           46 g           72 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaatt
//
LOCUS       DACCWV010000361          227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.374, whole genome shotgun sequence.
ACCESSION   DACCWV010000361 DACCWV010000000
VERSION     DACCWV010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.374"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            <1..>227
                     /locus_tag="IVW84_003070"
     CDS             <1..>227
                     /locus_tag="IVW84_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7919168.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWN"
BASE COUNT           91 a           38 c           42 g           56 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaat
//
LOCUS       DACCWV010000362          227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.375, whole genome shotgun sequence.
ACCESSION   DACCWV010000362 DACCWV010000000
VERSION     DACCWV010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.375"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            1..227
                     /locus_tag="IVW84_003071"
                     /pseudo
     CDS             1..227
                     /locus_tag="IVW84_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DACCWV010000363          224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.denovo.376, whole genome shotgun sequence.
ACCESSION   DACCWV010000363 DACCWV010000000
VERSION     DACCWV010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.denovo.376"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..197)
                     /locus_tag="IVW84_003072"
     CDS             complement(<1..197)
                     /locus_tag="IVW84_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919169.1"
                     /translation="MLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTL"
BASE COUNT           86 a           29 c           39 g           70 t
ORIGIN      
        1 taaagagtaa gccatggata aaaaatattc atcattgttc catgatgatc gaagttattg
       61 aagtttactg gagaactcca tacattatct agtcctatga ttcgagataa atgaaagtaa
      121 gtatcctgat tgacactatg atagattccc atagacatga atagagggat gacgtaagca
      181 aaacttagaa ataacataaa aatgatatct attatttggt tttt
//
LOCUS       DACCWV010000364        12125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.087, whole genome shotgun sequence.
ACCESSION   DACCWV010000364 DACCWV010000000
VERSION     DACCWV010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.087"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..717)
                     /locus_tag="IVW84_003073"
     CDS             complement(<1..717)
                     /locus_tag="IVW84_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7919170.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERI"
     gene            complement(678..1007)
                     /locus_tag="IVW84_003074"
     CDS             complement(678..1007)
                     /locus_tag="IVW84_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7919171.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(1265..1636)
                     /locus_tag="IVW84_003075"
     CDS             complement(1265..1636)
                     /locus_tag="IVW84_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7919172.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            1781..1966
                     /locus_tag="IVW84_003076"
     CDS             1781..1966
                     /locus_tag="IVW84_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7919173.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2909..3568
                     /locus_tag="IVW84_003077"
     CDS             2909..3568
                     /locus_tag="IVW84_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7919174.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            3571..4914
                     /gene="vanS"
                     /locus_tag="IVW84_003078"
     CDS             3571..4914
                     /gene="vanS"
                     /locus_tag="IVW84_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7919175.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            5085..5891
                     /gene="vanY"
                     /locus_tag="IVW84_003079"
     CDS             5085..5891
                     /gene="vanY"
                     /locus_tag="IVW84_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7919176.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            5909..6736
                     /gene="vanW"
                     /locus_tag="IVW84_003080"
     CDS             5909..6736
                     /gene="vanW"
                     /locus_tag="IVW84_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7919177.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            6733..7704
                     /locus_tag="IVW84_003081"
     CDS             6733..7704
                     /locus_tag="IVW84_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7919178.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            7697..8725
                     /gene="vanB"
                     /locus_tag="IVW84_003082"
     CDS             7697..8725
                     /gene="vanB"
                     /locus_tag="IVW84_003082"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7919179.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            8731..9339
                     /locus_tag="IVW84_003083"
     CDS             8731..9339
                     /locus_tag="IVW84_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7919180.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            9927..10358
                     /locus_tag="IVW84_003084"
     CDS             9927..10358
                     /locus_tag="IVW84_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7919181.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            10365..10598
                     /locus_tag="IVW84_003085"
     CDS             10365..10598
                     /locus_tag="IVW84_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7919182.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(10520..10762)
                     /locus_tag="IVW84_003086"
     CDS             complement(10520..10762)
                     /locus_tag="IVW84_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7919183.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            11019..11222
                     /locus_tag="IVW84_003087"
     CDS             11019..11222
                     /locus_tag="IVW84_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7919184.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            11306..>12125
                     /locus_tag="IVW84_003088"
     CDS             11306..>12125
                     /locus_tag="IVW84_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7919185.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIP"
BASE COUNT         3300 a         2687 c         3162 g         2976 t
ORIGIN      
        1 gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc agcgggtaaa
       61 gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct catagccttg
      121 cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag cggcgtcaat
      181 ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc actcggcata
      241 gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct tgcacagcct
      301 gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact tgcgctgatt
      361 taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat caatatgggt
      421 agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct tcccgatctc
      481 atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt ggtggcccag
      541 attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt ccgctgtttc
      601 ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg ggttctcgat
      661 gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa ctgccatatc
      721 tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc cataccgttc
      781 agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc ggtctgcgcc
      841 cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt ccgggcgtag
      901 gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc ccgttcctcc
      961 ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct tcaccgtcct
     1021 ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc cggcagggac
     1081 ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt tactgtttct
     1141 ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc tggaaagaaa
     1201 aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa acagaggatg
     1261 ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg gcgctgtcaa
     1321 tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg tggctgaccc
     1381 tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt tccacaggaa
     1441 gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc ccgtcgtttt
     1501 cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct ttgcgcgaaa
     1561 cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag ccgggacaat
     1621 cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac tctgcccgtg
     1681 gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt cttttattct
     1741 tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca tgacgttgaa
     1801 agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt gtgctgcggg
     1861 acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa ctgcggaaaa
     1921 gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata atgaaaccgg
     1981 cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac ggaaaaacta
     2041 taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc tgaaacagaa
     2101 agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca gaaatggaaa
     2161 atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt agaattgaaa
     2221 aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact gctggtctaa
     2281 tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct ttttgaggaa
     2341 aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa ggacacggat
     2401 gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg gtattaggac
     2461 tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca ggccctgcct
     2521 gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat tttgctgagg
     2581 cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg gtggatcaga
     2641 atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg ttttgacaac
     2701 cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat cttgatttaa
     2761 ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta tcccaatgac
     2821 ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac agactgttat
     2881 gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg tcgaggatga
     2941 tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc
     3001 ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat
     3061 tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa
     3121 aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc
     3181 gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa
     3241 gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag
     3301 gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac
     3361 acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca
     3421 tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca
     3481 cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg
     3541 tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt
     3601 ttttcctata cgatcattgc cctgctactg cttgtcggtg taacagcaac actgtttgca
     3661 cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag
     3721 ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg
     3781 tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc
     3841 tatgccacac caaatgccaa tacatcaaat agttttagac ccgactttct ttatgtggta
     3901 catagagatg ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat
     3961 caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta
     4021 tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg
     4081 aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc
     4141 gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg
     4201 gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca
     4261 gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt
     4321 gaaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg
     4381 gataggcagg gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga
     4441 atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat
     4501 tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa
     4561 attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca
     4621 gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa
     4681 tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg
     4741 ttcaccccat tctatcgcat tgatcaggcg caaagcagaa aaagtgggcg aagcggttta
     4801 ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac
     4861 acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt
     4921 aaaacttaaa tgattttgac cgacaggtat aaccctgccg gtcttttttt gctgctacag
     4981 gaaaactaca gactggctac agggaaagta cagatgcgct tgccataata acaatcgtac
     5041 cagccacaaa tcgtagtttt attgcaaagg aggcattcga tcaaatggaa aaaagcaact
     5101 atcattccaa tgcggatcat cacaaacggc atatgaaaca atccgtggaa aagcgggctt
     5161 ttctatgtgc gttcattatt tcgttcacag tctgcacgct gtttttgggg cggagactgg
     5221 cttccgtatt ggaggcaaca cagataccgc ccatccctgc aactcataca ggcagcagca
     5281 ctgacgtagt ggagaatttg gaggaaaacg ctcttgccac cgccaaagaa cagggagatg
     5341 aacaggaatg gagcctgatt ttagtgaaca ggcagaaccc catccccgca cagtacgatg
     5401 tggagcttga gcaactatca aatggtgagc ggatagatat tcggatttct ccctatcttc
     5461 aagatttgtt tgatgccgca agaactgatg gagtttaccc gattgtcgca tccggatacc
     5521 gaacaacaga aaaacagcaa gaaattatgg atgaaaaaat tgccgaatat aaggcgaaag
     5581 gctacacctc tgcacaggct aaagcggaag cagaaacttg ggtggccgtg ccgggaacga
     5641 gcgagcatca gcttggtctt gctgtggata tcaatgcgga cggaattcat tcaacaggca
     5701 acgaggttta tagatggctg gatgaaaaca gctatcgttt tggttttatt cgtcgctacc
     5761 caccagacaa gacagagata accggtgtga gcaacgagcc gtggcattac cgatatgttg
     5821 gcatcgaagc tgccacagag atgtacaacc aaggggtttg ccttgaggaa tatttaaaac
     5881 cagaaaaatg agaaaaggat gttatgttat ggacagaaaa agattgacac agcgtttccc
     5941 gttcctgctt ccaatgagac gggcgcagag aaaaatgtgc ttttatgcgg gaatgagatt
     6001 tgacggctgt cgctatgcgc agacgatagg ggaaaaatcg ctttcccatt tgctctttga
     6061 aacggattgc gcattataca accacaatac cggatttgac atgatatacc aggaaaacaa
     6121 ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc ttattgataa gaccggggga
     6181 acccttttct ttctggtggc tggtacgcca tgcggacaaa gataccccct ataaagacgg
     6241 ccttacggtg accaatggta aactcaccac catgtcgggc ggcggtatgt gccagatgag
     6301 caatttacta ttttggatgt tcctgcatac gccattgaca attatccagc gcagaggtca
     6361 cgaagtaaag gagtttccag agccaaacag tgacgagatt aaaggggtgg atgcaaccat
     6421 ttcagagggc tggattgatt taaaagtgcg aaacgatacc gactgcacct accaaatatg
     6481 ggtgacctta gatgatgaga aaatcatcgg tcaggtgtcc gccgacaaag agccccaagc
     6541 attatacaaa attacaaatg gcagtatcca gtatgtccgt gaaagtggcg ggatttatga
     6601 atatgcccag gttaaacgga tgcaagttgc cttaggtacc ggggaaataa tagattgcaa
     6661 gctgctttat acaaacaaat gcaaaatctg ttatcccctc ccggaaagtg tggatattca
     6721 ggaggagaac caatgagaaa aagtgtgggc attactgttt ttggatgcga acaggatgag
     6781 gcaaatgctt tccgcgcttt atcgccggat tttcatatta tccctacgct gattagcgat
     6841 gcgatatcgg cagacaacgc aaaattggcc gctggcaatc aatgcgttag cgtaggccat
     6901 aagtccgagg tttccgaggc gacaattctt gcgctgagaa aggtcggggt aaaatacatt
     6961 tctacccgca gcatcggctg cgatcacatt gatacgactg ccgccgagag aatgggaatc
     7021 tcggttggca cggttgcgta ttcgccggac agcgttgcgg attatgcttt gatgctgatg
     7081 ctgatggcca tacggggtgc aaaacccacc atgcacgccg tggcgcaaca agatttcaga
     7141 ttggatcgta tccgggggaa agaactgggg gatatgactg tgggagttat tggaaccggc
     7201 catatcgggc aagcggtcgt caaaaggctg cggggatttg gatgccatgt gctggcctat
     7261 gataacagcc gaaaaatgga tgcagattat gtccagcttg atgagcttct aaaaaacagc
     7321 gatattgtta cgctccatgt gccgctttgt gcggataccc gccatctgat cggtcagaag
     7381 caaattggag agatgaagca aggcgcattt ttaatcaaca ccgggcgcgg agcgcttgtc
     7441 gataccgggg cgctggtgga ggcgttggaa agcggaaagc tgggtggtgc ggcactggat
     7501 gtgttggagg gcgaggatca gtttgtttat accgattgct cgcagaaagt gcttgaccat
     7561 ccctttttgt cgcagctttt aaggatgccg aatgtgatca tcacacccca tacggcgtac
     7621 tacactgagc gtgtgctgca ggatactaca gaaaaaacaa tcaggaattg tctcaatttt
     7681 gaaaggagtt tacagcatga atagaataaa agtcgcaatc atcttcggcg gttgctcgga
     7741 ggaacatgat gtgtcggtaa aatccgcaat agaaattgct gcgaacattg atacggaaaa
     7801 attcgatccg cactacatcg gaattacaaa aaacggtgta tggaagctat gcaagaagcc
     7861 atgtacggaa tgggaagccg acagtctccc cgccatactc tccccggata ggaaaacgca
     7921 tgggctgctt gtcatgaaag aaagcgaata cgaaacacgg cgtattgatg tggctttccc
     7981 ggttttgcat ggcaaatgcg gggaggatgg tgcgatacag gggctgtttg tattgtctgg
     8041 tatcccctat gtgggctgtg atattcaaag ctccgcagct tgcatggaca aatcactggc
     8101 ctacattctt acaaaaaatg cgggcatcgc cgttcccgaa tttcaaatga ttgataaagg
     8161 tgacaagccg gaggcgggtg cgcttaccta ccctgtcttt gtgaagccgg cacggtcagg
     8221 ttcgtccttt ggcgtaacca aagtaaacgg tacggaagaa cttaacgctg cgatagaagc
     8281 ggcaggacaa tatgatggaa aaatcttaat tgagcaagcg atttcgggct gtgaggtcgg
     8341 gtgtgcggtc atggggaacg aggatgattt gattgtcggc gaagtggatc aaatccggct
     8401 gagccacggt accttccgca tccatcagga aaacgagccg gaaaaaggct cagaaaatgc
     8461 gatgattaca gttcccgcag acattccggt cgaggaacga aatcgggtgc aggaaacggc
     8521 aaagaaagta tatcgggtgc ttggatgcag agggcttgcc cgtgttgatc tttttttgca
     8581 ggaggatggc ggcatcgttc taaatgaggt caataccctg cccggtttta catcgtacag
     8641 ccgctaccca cgtatgatgg ccgccgcagg aatcacgctt cctgcactga ttgacagcct
     8701 gattacattg gcgttaaaga ggtgataagt atggaaaatg gttttttgtt tttagatgaa
     8761 atgttgcatg gcgtccgttg ggatgccaag tacgccacat gggataactt cacaggaaaa
     8821 ccggtggatg ggtatgaggt gaatcgcatc atcggcacaa aggccgtggc gtttgctctg
     8881 cgcgaggcac aaatccatgc ggctgcgctt ggctatggct tgcttttatg ggatggatat
     8941 cggccaagaa ctgcggtgga ctgcttcctg cgttgggcag cgcaaccgga ggacaatctc
     9001 acaaaagaaa aattttaccc caatatagag cgagccgagt tgattacaaa gggttatgtg
     9061 gcctcacaat ccagccatag ccgtggaagc gcaattgatc ttacgctcta ccacctggat
     9121 acaggggaac ttgtttcaat gggaagtaac ttcgatttta tggacgaacg gtcgcatcat
     9181 acagcaaaag ggatagggga tgcagaggca caaaatcgaa gatgcttgcg taaaatcatg
     9241 gaaagcagcg gatttcagtc ttatcgcttt gaatggtggc actataagtt gattgatgag
     9301 ccataccccg atacctattt taattttgcc gtttcataat gaaagtattg gttttgtatc
     9361 agattttgga tttgataaaa tctatgctgg tcatggacaa tcttttactg gaaagtggtt
     9421 aaagcatctt ttgtaataca ttgcaaatta tagtattcaa tctgcccagc ggcagaaaag
     9481 aaaaaaaccg ctgttgggca gacctttccc acgcccattt tgtattttgc tggcggcggt
     9541 tttgagaaat caaggccgcc gttcttttct ggcagcaaaa ggtgtggcgg cccataggag
     9601 gatgccgcta tgctgttttg ctattgccat gctctaatcc acttccacca ctcaaaaaaa
     9661 gccttgcccc tccacagggc tattccggtt atgagtatgt actataccat gtaaatcagc
     9721 agcatagcaa agcattttct cgcccggtag gtttgcgacc tgccgggcga tttttgtcgt
     9781 cttttgcggc ccggctgtcc ccaaaaaaat tttttgagaa attttcaaaa aggaggtatt
     9841 tagcgggtag ctggcggcct tgtcaaagtc ttgttagcgg aaagggagag aaccacctaa
     9901 gtcccccaca gaaagggggt gagaagatgg atgctatccc ccgcgactat gaagcgcggt
     9961 gcatgttcga tgctttttgc aagacggtac tgcgtaacga ggccaaaagc tacttggctg
    10021 aaatgaagcg ccgccgtgac cgtgaagtgt cactaagctc tttgtcacag gcagatttgg
    10081 acaagctctg tacagtagat cactacccca gcgacagctt tacgttttcg tcgcatggct
    10141 atgacctaca tatcaacaat gagcttgttg ccgaagcctt tgccgcgctg cccagcatgg
    10201 agcagagtat tttaatcctg cactgtgttc tggatatggc agatggtgag atcggcggcc
    10261 ttgtgggaat gtcccggagt gccgttcagc ggcacaggac aaatacctta tcggagctgc
    10321 ggaaacagct aaaggcgttg atgccgaagg gaggttagac gcggatgaag aacccgaact
    10381 acaagggcat tgagcttttg cccctttcgg tgatcgaggc cgcccgcgct ggggatgccg
    10441 gagccgtaga gcgcgttttg cagtattaca gcggttacat gaacaagctg tgtacccgga
    10501 cgctctatga cgaaaacggc tatccgcatg tgtgtctgga cgaggccatg aagcgccgtc
    10561 tggaaatcaa gctcatccac gctatcgtca cgaactgacg aaccggcccc ggcagcatga
    10621 aacggcttac cctttccccg tttcggtgtt ttcccggtgg cctgatcgca ccttgataaa
    10681 tatgcccgca agataacggc ggcgcatcat agggcatacg gacacattct gtctgtgccg
    10741 agccggacgg ctggtgcgcc atgatacttt tccctacggg aaaagcggag cgaggaacac
    10801 cacgccgcaa tgtgagcgtg gcggcttgcg ggcgacgacg tacaggcagg actatgatac
    10861 tcccttatag ccacagtccg agcgttaaaa gcgtcgcagg caatgggtag ggccgcgtga
    10921 gaaccacgcg ggggtgaaag tcccgtggag ctgtgccagc agccgtccgt ctatgcccct
    10981 aaatcacaca aacctttgaa tttgtgaaag ggggctgcat tatgaaccat gatgtgccta
    11041 tttgggaaaa atacacgctg acgattgagg aagcgtctaa atatttccgt atcggagaaa
    11101 acaaattgcg taagctggcc gaggaaaatc cggcctctgg ctgggtcatt atgaatggca
    11161 accgcatcca gatcaaacgc aagcagtttg agaaaataat tgacactttg gacacaatct
    11221 aatagtgaaa aagggccgca gatatgttat aataatttat aagcatatat gcggctcttt
    11281 ccgtcatgga aaggagcatg acgagatgtc agaaaaaaga cgagacagca aaaatcgcgt
    11341 tttgcggtct ggagagagcc agagaaaaga cggacgatat gcttacaaat atgttgatac
    11401 ctttggaaaa ccgcagtttg tttactcatg gaagttagtg cctacggata agaccccggc
    11461 aggtaaacgg gaggatattt cactccggga aaaggagaaa gaaatccaga aagaccttga
    11521 cgacgggata gacaccatcg gcaagaaaat gaccgtctgc cagctctacg ccaagcagat
    11581 caggcaccgg ggcaatgtga agcacaacac caaactgggc cgggaacggc tgatgcgtat
    11641 tctggaacaa gatcggctgg ggagctgccc gattgacagt gtgaagctgt ccgacgcaaa
    11701 ggaatgggcg cttcggatga aagaaaaggg cctttcttat aagaccatca ataacgacaa
    11761 gcgttccctg aaagcggctt tttacaccgc catacaggac gattgcatcc gaaaaaaccc
    11821 gtttgacttc cagcttagtg acgttctgga cgacgatacg gaaccaaagg tgcctcttac
    11881 accagcacag gaagaaagtt ttctgtcctt tattcaaggt gataaggttt accaaaagca
    11941 ttatgacgca atcgttattc tgctggggac agggcttcgc atttcggaac tgtgcggact
    12001 gacggacaaa gatttggatt ttgaaaaccg ggtaatcatc gtgagccacc agcttttgag
    12061 aaataccggc gtcggttact atattgacga acccaagacc caaagcggcg tcagaaaaat
    12121 cccca
//
LOCUS       DACCWV010000365         9973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.102, whole genome shotgun sequence.
ACCESSION   DACCWV010000365 DACCWV010000000
VERSION     DACCWV010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.102"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            complement(133..1005)
                     /locus_tag="IVW84_003089"
     CDS             complement(133..1005)
                     /locus_tag="IVW84_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7919186.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1133..1702
                     /locus_tag="IVW84_003090"
     CDS             1133..1702
                     /locus_tag="IVW84_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7919187.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1769..2506
                     /locus_tag="IVW84_003091"
     CDS             1769..2506
                     /locus_tag="IVW84_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7919188.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2737..4005
                     /locus_tag="IVW84_003092"
     CDS             2737..4005
                     /locus_tag="IVW84_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7919189.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4492..4956
                     /locus_tag="IVW84_003093"
     CDS             4492..4956
                     /locus_tag="IVW84_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7919190.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4940..5569
                     /locus_tag="IVW84_003094"
     CDS             4940..5569
                     /locus_tag="IVW84_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7919191.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            5923..7401
                     /gene="msr"
                     /locus_tag="IVW84_003095"
     CDS             5923..7401
                     /gene="msr"
                     /locus_tag="IVW84_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7919192.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7448..7894
                     /locus_tag="IVW84_003096"
     CDS             7448..7894
                     /locus_tag="IVW84_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7919193.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8093..8608)
                     /locus_tag="IVW84_003097"
     CDS             complement(8093..8608)
                     /locus_tag="IVW84_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7919194.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8725..9927
                     /locus_tag="IVW84_003098"
     CDS             8725..9927
                     /locus_tag="IVW84_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7919195.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3178 a         1747 c         2076 g         2972 t
ORIGIN      
        1 tatagtaatt tactggaatt atcagaaata aaaggaagaa ataaattaag aaacttatgt
       61 tatttaatcg ttggatcttt tgcttatctt attcgttgtt aaaaagctga aagcgaagga
      121 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
      181 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
      241 tgggatgaat attttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
      301 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
      361 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
      421 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgacgg tttgctttgc
      481 taaaggatga tttcgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
      541 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
      601 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
      661 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
      721 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
      781 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
      841 tatctctcgg gagccccgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
      901 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
      961 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     1021 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     1081 ttagacatga taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     1141 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     1201 gatccatcag atccaagctg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     1261 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     1321 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     1381 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     1441 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     1501 acgaagaggc ttgttagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     1561 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     1621 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     1681 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     1741 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     1801 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     1861 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     1921 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     1981 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     2041 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     2101 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     2161 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     2221 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     2281 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     2341 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     2401 attgacctcc gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     2461 gtctgtcagt aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     2521 tatccaaaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     2581 tggagaagaa tattcttcat ttacgtcttt ttatgtagaa aaagcaaatt tatcaaaaag
     2641 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     2701 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     2761 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     2821 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     2881 gagagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     2941 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     3001 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     3061 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     3121 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     3181 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     3241 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     3301 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     3361 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     3421 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     3481 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     3541 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     3601 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     3661 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     3721 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     3781 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     3841 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     3901 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     3961 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     4021 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     4081 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     4141 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     4201 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattttct
     4261 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     4321 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     4381 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     4441 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     4501 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     4561 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     4621 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     4681 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     4741 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat cgagcattgt
     4801 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     4861 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     4921 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     4981 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     5041 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg caaccatggc gctttgtggt
     5101 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     5161 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     5221 cgaagaaatc tataaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     5281 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     5341 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     5401 ctatgaaaca aatccaattg gtggagtcga agcagatcag ttggcagagg catttggttt
     5461 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     5521 ttacgagtct gttcgtttag caccagaaaa gatcactacg ttcgaataaa aggttttcag
     5581 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     5641 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     5701 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     5761 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     5821 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     5881 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     5941 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     6001 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     6061 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     6121 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     6181 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     6241 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     6301 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     6361 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     6421 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     6481 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     6541 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     6601 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac cggatcggtt gtcttcctct
     6661 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     6721 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
     6781 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
     6841 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
     6901 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
     6961 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
     7021 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
     7081 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
     7141 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
     7201 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
     7261 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
     7321 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
     7381 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
     7441 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
     7501 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
     7561 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
     7621 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
     7681 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
     7741 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
     7801 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
     7861 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
     7921 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
     7981 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
     8041 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
     8101 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
     8161 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
     8221 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
     8281 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
     8341 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
     8401 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
     8461 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
     8521 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
     8581 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
     8641 tatatcacag taaagtttcc acgttttaca tgtgcatatt tacatgtaaa ataaatcatg
     8701 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
     8761 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
     8821 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
     8881 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
     8941 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
     9001 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
     9061 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
     9121 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
     9181 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
     9241 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
     9301 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
     9361 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
     9421 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
     9481 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
     9541 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
     9601 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
     9661 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
     9721 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
     9781 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
     9841 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
     9901 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
     9961 caagtcataa gaa
//
LOCUS       DACCWV010000366         5734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.156, whole genome shotgun sequence.
ACCESSION   DACCWV010000366 DACCWV010000000
VERSION     DACCWV010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.156"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            225..1121
                     /locus_tag="IVW84_003099"
     CDS             225..1121
                     /locus_tag="IVW84_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7919196.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1271..2212)
                     /locus_tag="IVW84_003100"
     CDS             complement(1271..2212)
                     /locus_tag="IVW84_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7919197.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2338..3459
                     /locus_tag="IVW84_003101"
     CDS             2338..3459
                     /locus_tag="IVW84_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7919198.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3717..5219
                     /locus_tag="IVW84_003102"
     CDS             3717..5219
                     /locus_tag="IVW84_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7919199.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5286..>5734
                     /locus_tag="IVW84_003103"
     CDS             5286..>5734
                     /locus_tag="IVW84_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7919200.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVI"
BASE COUNT         1900 a         1016 c         1202 g         1616 t
ORIGIN      
        1 gaataaatag caatatttaa caatgactaa tgataaaaaa ttttcagaca attacaacta
       61 ttcagaaaac ttttaggcga attttaatag aagaatgatt tatttcatca tattttcata
      121 caaatgttaa attttcatac aaatatagga aacgttttac tggcttttta ctatttaaac
      181 aggctatcat aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg
      241 tactgctagt tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg
      301 tttggtccgg aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg
      361 caaattatca gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg
      421 tgcgcccatt taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac
      481 acgaggcgat gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg
      541 attttttgga agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata
      601 tgaatttttc ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga
      661 gaaaagaacg agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact
      721 atttcattct tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc
      781 tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag
      841 tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag
      901 cgattgctca ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt
      961 tagatgaatt accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg
     1021 aattttatga tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg
     1081 agcaatacga atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga
     1141 gattatgtac tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt
     1201 tttgatcttt tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc
     1261 agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa
     1321 aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga
     1381 tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat
     1441 gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat
     1501 ctagtatgat tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca
     1561 tgctctctgc cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata
     1621 aaccattcaa tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc
     1681 tatagatcgg atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt
     1741 cgccttctgg ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc
     1801 cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca
     1861 catatttttc cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat
     1921 tgtcagtaat aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta
     1981 atgctacgat caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg
     2041 gcagttttgc gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg
     2101 cttcaatgtg agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca
     2161 tcaatccgat tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc
     2221 tctcctttat tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt
     2281 tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg
     2341 gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag
     2401 gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat
     2461 ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt
     2521 gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa
     2581 ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa
     2641 ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat
     2701 gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat
     2761 ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat
     2821 cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta
     2881 aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg
     2941 caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca
     3001 ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga
     3061 agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct
     3121 ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc
     3181 atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt
     3241 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
     3301 caagagctag aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca
     3361 ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga
     3421 cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta
     3481 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     3541 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
     3601 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
     3661 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
     3721 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
     3781 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     3841 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
     3901 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
     3961 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
     4021 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
     4081 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
     4141 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
     4201 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
     4261 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
     4321 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
     4381 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
     4441 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
     4501 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     4561 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     4621 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     4681 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     4741 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     4801 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     4861 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     4921 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     4981 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     5041 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     5101 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     5161 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     5221 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     5281 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     5341 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     5401 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     5461 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     5521 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     5581 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     5641 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     5701 tagtttgcca taatgccttg ataagagtga tcaa
//
LOCUS       DACCWV010000367         5287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.162, whole genome shotgun sequence.
ACCESSION   DACCWV010000367 DACCWV010000000
VERSION     DACCWV010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.162"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            3..188
                     /locus_tag="IVW84_003104"
     CDS             3..188
                     /locus_tag="IVW84_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7919201.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="IVW84_003105"
     CDS             203..1072
                     /locus_tag="IVW84_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7919202.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="IVW84_003106"
     CDS             1053..1787
                     /locus_tag="IVW84_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7919203.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="IVW84_003107"
     CDS             1820..2728
                     /locus_tag="IVW84_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7919204.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3267
                     /gene="sat4"
                     /locus_tag="IVW84_003108"
     CDS             2725..3267
                     /gene="sat4"
                     /locus_tag="IVW84_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7919205.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3360..4154
                     /locus_tag="IVW84_003109"
                     /note="aph(3')-IIIa"
     CDS             3360..4154
                     /locus_tag="IVW84_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7919206.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(4262..4450)
                     /locus_tag="IVW84_003110"
                     /pseudo
     CDS             complement(4262..4450)
                     /locus_tag="IVW84_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4430..5002
                     /locus_tag="IVW84_003111"
     CDS             4430..5002
                     /locus_tag="IVW84_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7919207.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         1821 a          795 c         1186 g         1485 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     3001 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     3061 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     3121 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     3181 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3241 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3301 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     3601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     3661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     3721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     3781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     3841 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     3901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     3961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4141 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     4201 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     4261 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     4321 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     4381 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     4441 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     4501 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     4561 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     4621 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     4681 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     4741 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     4801 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     4861 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     4921 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     4981 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     5041 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     5101 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     5161 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
     5221 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
     5281 ttgtgat
//
LOCUS       DACCWV010000368         5205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.164, whole genome shotgun sequence.
ACCESSION   DACCWV010000368 DACCWV010000000
VERSION     DACCWV010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.164"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            132..2018
                     /locus_tag="IVW84_003112"
     CDS             132..2018
                     /locus_tag="IVW84_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7919208.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2030..2977
                     /locus_tag="IVW84_003113"
     CDS             2030..2977
                     /locus_tag="IVW84_003113"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7919209.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2997..3524
                     /locus_tag="IVW84_003114"
     CDS             2997..3524
                     /locus_tag="IVW84_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7919210.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3587..4240)
                     /locus_tag="IVW84_003115"
     CDS             complement(3587..4240)
                     /locus_tag="IVW84_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7919211.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4372..>5205
                     /locus_tag="IVW84_003116"
     CDS             4372..>5205
                     /locus_tag="IVW84_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7919212.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YY"
BASE COUNT         1868 a          909 c         1100 g         1328 t
ORIGIN      
        1 aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa
       61 gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat
      121 aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa
      181 aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg
      241 ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg
      301 acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag
      361 cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct
      421 tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag
      481 accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt
      541 ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata
      601 aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga
      661 ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga
      721 tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg
      781 atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat
      841 acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag
      901 aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg
      961 ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa
     1021 aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac
     1081 gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac
     1141 tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa
     1201 atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg
     1261 gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa
     1321 tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag
     1381 cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta
     1441 tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga
     1501 aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca
     1561 tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg
     1621 tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa
     1681 acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga
     1741 cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga
     1801 agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac
     1861 ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc
     1921 gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg
     1981 cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc
     2041 gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg
     2101 cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc
     2161 tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt
     2221 aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg
     2281 ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg
     2341 ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt
     2401 ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat
     2461 ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa
     2521 acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg
     2581 gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta
     2641 tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg
     2701 cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg
     2761 tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat
     2821 cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa
     2881 tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga
     2941 cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt
     3001 tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt
     3061 ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga
     3121 tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg
     3181 ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag
     3241 acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg
     3301 tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat
     3361 tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta
     3421 gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta
     3481 atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga
     3541 ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata
     3601 aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca
     3661 tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg
     3721 ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga
     3781 cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt
     3841 tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa
     3901 ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg
     3961 caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa
     4021 aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga
     4081 tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca
     4141 ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat
     4201 ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag
     4261 gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat
     4321 gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat
     4381 gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc
     4441 attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa
     4501 ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc
     4561 caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa
     4621 gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc
     4681 agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta
     4741 tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc
     4801 cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta
     4861 gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg
     4921 aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga
     4981 ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca
     5041 aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa
     5101 gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt
     5161 atcgctacgc atactggtaa agacgcgttt gcaatcatgt attat
//
LOCUS       DACCWV010000369         2771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.215, whole genome shotgun sequence.
ACCESSION   DACCWV010000369 DACCWV010000000
VERSION     DACCWV010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.215"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            110..193
                     /locus_tag="IVW84_003117"
     CDS             110..193
                     /locus_tag="IVW84_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7919213.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="IVW84_003118"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="IVW84_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7919214.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="IVW84_003119"
     CDS             complement(1318..2181)
                     /locus_tag="IVW84_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7919215.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="IVW84_003120"
     CDS             complement(2183..2455)
                     /locus_tag="IVW84_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7919216.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="IVW84_003121"
     CDS             complement(2472..2681)
                     /locus_tag="IVW84_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7919217.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DACCWV010000370         2766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.216, whole genome shotgun sequence.
ACCESSION   DACCWV010000370 DACCWV010000000
VERSION     DACCWV010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.216"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            3..188
                     /locus_tag="IVW84_003122"
     CDS             3..188
                     /locus_tag="IVW84_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7919218.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="IVW84_003123"
     CDS             203..1072
                     /locus_tag="IVW84_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7919219.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="IVW84_003124"
     CDS             1053..1787
                     /locus_tag="IVW84_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7919220.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2740
                     /gene="ant(6)"
                     /locus_tag="IVW84_003125"
     CDS             1820..2740
                     /gene="ant(6)"
                     /locus_tag="IVW84_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAP7919221.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQGLQK"
BASE COUNT          961 a          389 c          616 g          800 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagggatt acagaaatga aagcagggca cctgaaagat
     2761 atcgat
//
LOCUS       DACCWV010000371         1238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.286, whole genome shotgun sequence.
ACCESSION   DACCWV010000371 DACCWV010000000
VERSION     DACCWV010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.286"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            6..794
                     /gene="aph(2'')-I"
                     /locus_tag="IVW84_003126"
                     /pseudo
     CDS             6..794
                     /gene="aph(2'')-I"
                     /locus_tag="IVW84_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            778..1170
                     /locus_tag="IVW84_003127"
     CDS             778..1170
                     /locus_tag="IVW84_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7919222.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          506 a           89 c          205 g          438 t
ORIGIN      
        1 atttaattga gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg
       61 gttatgatag tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta
      121 ctaataagaa aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt
      181 tagaaactaa tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta
      241 tactaggtta taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt
      301 cagaagaaga acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg
      361 gtttagatta tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag
      421 agtatatatt gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata
      481 tagaaagttt tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat
      541 gccataatga ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa
      601 taattgattt tggagattct ggaattatag atgaatattg tgattttata tacttacttg
      661 aagatagtga agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa
      721 atatagatat tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg
      781 aaactattgt ttattgatga taatatggtt tttgatattg ataatttaaa aggatttctt
      841 aatgatacca gttcatttgg gtttatagct aaagaaaata ataaaattat aggatttgca
      901 tattgctata cacttttaag acctgatgga aaaacaatgt tttatttaca ctcaatagga
      961 atgttaccta actatcaaga caaaggttat ggttcaaaat tattatcttt tattaaggaa
     1021 tattctaaag agattggttg ttctgaaatg tttttaataa ctgataaagg taatcctaga
     1081 gcttgccatg tatatgaaaa attaggtggt aaaaatgatt ataaagatga aatagtatat
     1141 gtatatgatt atgaaaaagg tgataaataa atgaatatag ttgaaaatga aatatgtata
     1201 agaactttaa tagatgatga ttttcctttg atgttaaa
//
LOCUS       DACCWV010000372          745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.316, whole genome shotgun sequence.
ACCESSION   DACCWV010000372 DACCWV010000000
VERSION     DACCWV010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.316"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            132..680
                     /gene="aac(6')"
                     /locus_tag="IVW84_003128"
     CDS             132..680
                     /gene="aac(6')"
                     /locus_tag="IVW84_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7919223.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          272 a          110 c          166 g          197 t
ORIGIN      
        1 ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa
       61 gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag
      121 ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc
      181 tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag
      241 tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag
      301 taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag
      361 ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa
      421 aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg
      481 gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc
      541 agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg
      601 tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc
      661 ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat
      721 aagaaggaat ttacgaaaat tactt
//
LOCUS       DACCWV010000373          583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW16158
            SAMN14604032-rid9902913.guided.325, whole genome shotgun sequence.
ACCESSION   DACCWV010000373 DACCWV010000000
VERSION     DACCWV010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14604032
            Sequence Read Archive: SRR11551397
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000744133.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000744133.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:59:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,129
            CDSs (total)                      :: 3,070
            Genes (coding)                    :: 2,987
            CDSs (with protein)               :: 2,987
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 36 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 6 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14604032-rid9902913.guided.325"
                     /isolate="UW16158"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Lower Saxony"
                     /collection_date="2017"
                     /collected_by="Robert Koch Institut"
     gene            12..506
                     /gene="aac(6')-I"
                     /locus_tag="IVW84_003129"
                     /pseudo
     CDS             12..506
                     /gene="aac(6')-I"
                     /locus_tag="IVW84_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
BASE COUNT          240 a           50 c          105 g          188 t
ORIGIN      
        1 taagaacttt aatagatgat gattttcctt tgatgttaaa atggttaact gatgaaagag
       61 tattagaatt ttatgatggt agagataaaa aatatacatt agaatcatta aaaaaacatt
      121 atacagagcc ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta
      181 ttggatatgg acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa
      241 aaactgatga gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta
      301 aaggaattgg tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg
      361 ctaatgcagt tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa
      421 aatctggttt tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag
      481 aagattgtta tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga
      541 aatatttaat tgagcattac tttgataatt tcaaagtaga tag
//
